Base de dados : MEDLINE
Pesquisa : D02.065.327 [Categoria DeCS]
Referências encontradas : 462 [refinar]
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[PMID]:29176835
[Au] Autor:Zhang J; Burgess JG
[Ad] Endereço:Institute of Biomedical and Clinical Sciences, University of Exeter Medical School, Hatherly Laboratory, Exeter, United Kingdom.
[Ti] Título:Enhanced eicosapentaenoic acid production by a new deep-sea marine bacterium Shewanella electrodiphila MAR441T.
[So] Source:PLoS One;12(11):e0188081, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Omega-3 fatty acids are products of secondary metabolism, essential for growth and important for human health. Although there are numerous reports of bacterial production of omega-3 fatty acids, less information is available on the biotechnological production of these compounds from bacteria. The production of eicosapentaenoic acid (EPA, 20:5ω3) by a new species of marine bacteria Shewanella electrodiphila MAR441T was investigated under different fermentation conditions. This strain produced a high percentage (up to 26%) of total fatty acids and high yields (mg / g of biomass) of EPA at or below the optimal growth temperature. At higher growth temperatures these values decreased greatly. The amount of EPA produced was affected by the carbon source, which also influenced fatty acid composition. This strain required Na+ for growth and EPA synthesis and cells harvested at late exponential or early stationary phase had a higher EPA content. Both the highest amounts (20 mg g-1) and highest percent EPA content (18%) occurred with growth on L-proline and (NH4)2SO4. The addition of cerulenin further enhanced EPA production to 30 mg g-1. Chemical mutagenesis using NTG allowed the isolation of mutants with improved levels of EPA content (from 9.7 to 15.8 mg g-1) when grown at 15°C. Thus, the yields of EPA could be substantially enhanced without the need for recombinant DNA technology, often a commercial requirement for food supplement manufacture.
[Mh] Termos MeSH primário: Organismos Aquáticos/metabolismo
Ácido Eicosapentaenoico/biossíntese
Oceanos e Mares
Shewanella/metabolismo
[Mh] Termos MeSH secundário: Organismos Aquáticos/efeitos dos fármacos
Organismos Aquáticos/crescimento & desenvolvimento
Biomassa
Carbono/farmacologia
Cerulenina/farmacologia
Mutação/genética
Nitrogênio/farmacologia
Fosfolipídeos/metabolismo
Shewanella/efeitos dos fármacos
Shewanella/crescimento & desenvolvimento
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Phospholipids); 17397-89-6 (Cerulenin); 7440-44-0 (Carbon); AAN7QOV9EA (Eicosapentaenoic Acid); N762921K75 (Nitrogen)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171219
[Lr] Data última revisão:
171219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171128
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0188081


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[PMID]:28542493
[Au] Autor:Nickels JD; Chatterjee S; Stanley CB; Qian S; Cheng X; Myles DAA; Standaert RF; Elkins JG; Katsaras J
[Ad] Endereço:Shull Wollan Center-A Joint Institute for Neutron Sciences, Oak Ridge National Laboratory, Oak Ridge, Tennessee, United States of America.
[Ti] Título:The in vivo structure of biological membranes and evidence for lipid domains.
[So] Source:PLoS Biol;15(5):e2002214, 2017 May.
[Is] ISSN:1545-7885
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Examining the fundamental structure and processes of living cells at the nanoscale poses a unique analytical challenge, as cells are dynamic, chemically diverse, and fragile. A case in point is the cell membrane, which is too small to be seen directly with optical microscopy and provides little observational contrast for other methods. As a consequence, nanoscale characterization of the membrane has been performed ex vivo or in the presence of exogenous labels used to enhance contrast and impart specificity. Here, we introduce an isotopic labeling strategy in the gram-positive bacterium Bacillus subtilis to investigate the nanoscale structure and organization of its plasma membrane in vivo. Through genetic and chemical manipulation of the organism, we labeled the cell and its membrane independently with specific amounts of hydrogen (H) and deuterium (D). These isotopes have different neutron scattering properties without altering the chemical composition of the cells. From neutron scattering spectra, we confirmed that the B. subtilis cell membrane is lamellar and determined that its average hydrophobic thickness is 24.3 ± 0.9 Ångstroms (Å). Furthermore, by creating neutron contrast within the plane of the membrane using a mixture of H- and D-fatty acids, we detected lateral features smaller than 40 nm that are consistent with the notion of lipid rafts. These experiments-performed under biologically relevant conditions-answer long-standing questions in membrane biology and illustrate a fundamentally new approach for systematic in vivo investigations of cell membrane structure.
[Mh] Termos MeSH primário: Bacillus subtilis/metabolismo
Membrana Celular/metabolismo
Ácidos Graxos/metabolismo
Bicamadas Lipídicas/metabolismo
Microdomínios da Membrana/metabolismo
Modelos Biológicos
[Mh] Termos MeSH secundário: Algoritmos
Bacillus subtilis/química
Bacillus subtilis/efeitos dos fármacos
Bacillus subtilis/crescimento & desenvolvimento
Proteínas de Bactérias/genética
Proteínas de Bactérias/metabolismo
Membrana Celular/química
Membrana Celular/efeitos dos fármacos
Cerulenina/farmacologia
Deutério
Enoil-CoA Hidratase/genética
Enoil-CoA Hidratase/metabolismo
Inibidores da Síntese de Ácidos Graxos/farmacologia
Ácidos Graxos/química
Deleção de Genes
Interações Hidrofóbicas e Hidrofílicas
Bicamadas Lipídicas/química
Microdomínios da Membrana/química
Microdomínios da Membrana/efeitos dos fármacos
Viabilidade Microbiana/efeitos dos fármacos
Difração de Nêutrons
Ácidos Palmíticos/química
Ácidos Palmíticos/metabolismo
Espalhamento a Baixo Ângulo
Estereoisomerismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Fatty Acid Synthesis Inhibitors); 0 (Fatty Acids); 0 (Lipid Bilayers); 0 (Palmitic Acids); 17397-89-6 (Cerulenin); 5502-94-3 (aseanostatin P5); AR09D82C7G (Deuterium); EC 4.2.1.17 (Enoyl-CoA Hydratase)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170526
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pbio.2002214


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[PMID]:27918556
[Au] Autor:Bastos DC; Paupert J; Maillard C; Seguin F; Carvalho MA; Agostini M; Coletta RD; Noël A; Graner E
[Ad] Endereço:Department of Oral Diagnosis, School of Dentistry of Piracicaba, State University of Campinas (UNICAMP), Piracicaba, Brazil.
[Ti] Título:Effects of fatty acid synthase inhibitors on lymphatic vessels: an in vitro and in vivo study in a melanoma model.
[So] Source:Lab Invest;97(2):194-206, 2017 Feb.
[Is] ISSN:1530-0307
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Fatty acid synthase (FASN) is responsible for the endogenous production of fatty acids from acetyl-CoA and malonyl-CoA. Its overexpression is associated with poor prognosis in human cancers including melanomas. Our group has previously shown that the inhibition of FASN with orlistat reduces spontaneous lymphatic metastasis in experimental B16-F10 melanomas, which is a consequence, at least in part, of the reduction of proliferation and induction of apoptosis. Here, we sought to investigate the effects of pharmacological FASN inhibition on lymphatic vessels by using cell culture and mouse models. The effects of FASN inhibitors cerulenin and orlistat on the proliferation, apoptosis, and migration of human lymphatic endothelial cells (HDLEC) were evaluated with in vitro models. The lymphatic outgrowth was evaluated by using a murine ex vivo assay. B16-F10 melanomas and surgical wounds were produced in the ears of C57Bl/6 and Balb-C mice, respectively, and their peripheral lymphatic vessels evaluated by fluorescent microlymphangiography. The secretion of vascular endothelial growth factor C and D (VEGF-C and -D) by melanoma cells was evaluated by ELISA and conditioned media used to study in vitro lymphangiogenesis. Here, we show that cerulenin and orlistat decrease the viability, proliferation, and migration of HDLEC cells. The volume of lymph node metastases from B16-F10 experimental melanomas was reduced by 39% in orlistat-treated animals as well as the expression of VEGF-C in these tissues. In addition, lymphatic vessels from orlistat-treated mice drained more efficiently the injected FITC-dextran. Orlistat and cerulenin reduced VEGF-C secretion and, increase production of VEGF-D by B16-F10 and SK-Mel-25 melanoma cells. Finally, reduced lymphatic cell extensions, were observed following the treatment with conditioned medium from cerulenin- and orlistat-treated B16-F10 cells. Altogether, our results show that FASN inhibitors have anti-metastatic effects by acting on lymphatic endothelium and melanoma cells regardless the increase of lymphatic permeability promoted by orlistat.
[Mh] Termos MeSH primário: Cerulenina/farmacologia
Ácido Graxo Sintases/antagonistas & inibidores
Lactonas/farmacologia
Vasos Linfáticos/efeitos dos fármacos
Melanoma Experimental/prevenção & controle
[Mh] Termos MeSH secundário: Animais
Apoptose/efeitos dos fármacos
Western Blotting
Linhagem Celular
Linhagem Celular Tumoral
Movimento Celular/efeitos dos fármacos
Proliferação Celular/efeitos dos fármacos
Sobrevivência Celular/efeitos dos fármacos
Ácido Graxo Sintases/genética
Ácido Graxo Sintases/metabolismo
Inibidores da Síntese de Ácidos Graxos/farmacologia
Seres Humanos
Linfangiogênese/efeitos dos fármacos
Metástase Linfática
Vasos Linfáticos/metabolismo
Melanoma/genética
Melanoma/metabolismo
Melanoma/patologia
Melanoma Experimental/genética
Melanoma Experimental/metabolismo
Camundongos Endogâmicos BALB C
Camundongos Endogâmicos C57BL
Interferência de RNA
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Fator C de Crescimento do Endotélio Vascular/metabolismo
Fator D de Crescimento do Endotélio Vascular/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fatty Acid Synthesis Inhibitors); 0 (Lactones); 0 (Vascular Endothelial Growth Factor C); 0 (Vascular Endothelial Growth Factor D); 17397-89-6 (Cerulenin); 95M8R751W8 (orlistat); EC 2.3.1.85 (Fatty Acid Synthases)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170621
[Lr] Data última revisão:
170621
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161206
[St] Status:MEDLINE
[do] DOI:10.1038/labinvest.2016.125


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[PMID]:27875634
[Au] Autor:Pulschen AA; Sastre DE; Machinandiarena F; Crotta Asis A; Albanesi D; de Mendoza D; Gueiros-Filho FJ
[Ad] Endereço:Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, Brazil.
[Ti] Título:The stringent response plays a key role in Bacillus subtilis survival of fatty acid starvation.
[So] Source:Mol Microbiol;103(4):698-712, 2017 Feb.
[Is] ISSN:1365-2958
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The stringent response is a universal adaptive mechanism to protect bacteria from nutritional and environmental stresses. The role of the stringent response during lipid starvation has been studied only in Gram-negative bacteria. Here, we report that the stringent response also plays a crucial role in the adaptation of the model Gram-positive Bacillus subtilis to fatty acid starvation. B. subtilis lacking all three (p)ppGpp-synthetases (Rel , RelP and RelQ) or bearing a Rel variant that no longer synthesizes (p)ppGpp suffer extreme loss of viability on lipid starvation. Loss of viability is paralleled by perturbation of membrane integrity and function, with collapse of membrane potential as the likely cause of death. Although no increment of (p)ppGpp could be detected in lipid starved B. subtilis, we observed a substantial increase in the GTP/ATP ratio of strains incapable of synthesizing (p)ppGpp. Artificially lowering GTP with decoyinine rescued viability of such strains, confirming observations that low intracellular GTP is important for survival of nutritional stresses. Altogether, our results show that activation of the stringent response by lipid starvation is a broadly conserved response of bacteria and that a key role of (p)ppGpp is to couple biosynthetic processes that become detrimental if uncoordinated.
[Mh] Termos MeSH primário: Trifosfato de Adenosina/metabolismo
Bacillus subtilis/crescimento & desenvolvimento
Bacillus subtilis/metabolismo
Ácidos Graxos/metabolismo
Guanosina Trifosfato/metabolismo
Ligases/genética
Potenciais da Membrana/fisiologia
Inanição/metabolismo
[Mh] Termos MeSH secundário: Cerulenina/farmacologia
Inibidores da Síntese de Ácidos Graxos/farmacologia
Ácidos Graxos/biossíntese
Estresse Fisiológico
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fatty Acid Synthesis Inhibitors); 0 (Fatty Acids); 17397-89-6 (Cerulenin); 86-01-1 (Guanosine Triphosphate); 8L70Q75FXE (Adenosine Triphosphate); EC 6.- (Ligases); EC 6.- (guanosine 3',5'-polyphosphate synthetases)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170816
[Lr] Data última revisão:
170816
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161123
[St] Status:MEDLINE
[do] DOI:10.1111/mmi.13582


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[PMID]:27649078
[Au] Autor:Peng YF; Chen WC; Xiao K; Xu L; Wang L; Wan X
[Ad] Endereço:Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan, China.
[Ti] Título:DHA Production in Escherichia coli by Expressing Reconstituted Key Genes of Polyketide Synthase Pathway from Marine Bacteria.
[So] Source:PLoS One;11(9):e0162861, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The gene encoding phosphopantetheinyl transferase (PPTase), pfaE, a component of the polyketide synthase (PKS) pathway, is crucial for the production of docosahexaenoic acid (DHA, 22:6ω3), along with the other pfa cluster members pfaA, pfaB, pfaC and pfaD. DHA was produced in Escherichia coli by co-expressing pfaABCD from DHA-producing Colwellia psychrerythraea 34H with one of four pfaE genes from bacteria producing arachidonic acid (ARA, 20:4ω6), eicosapentaenoic acid (EPA, 20:5ω3) or DHA, respectively. Substitution of the pfaE gene from different strain source in E. coli did not influence the function of the PKS pathway producing DHA, although they led to different DHA yields and fatty acid profiles. This result suggested that the pfaE gene could be switchable between these strains for the production of DHA. The DHA production by expressing the reconstituted PKS pathway was also investigated in different E. coli strains, at different temperatures, or with the treatment of cerulenin. The highest DHA production, 2.2 mg of DHA per gram of dry cell weight or 4.1% of total fatty acids, was obtained by co-expressing pfaE(EPA) from the EPA-producing strain Shewanella baltica with pfaABCD in DH5α. Incubation at low temperature (10-15°C) resulted in higher accumulation of DHA compared to higher temperatures. The addition of cerulenin to the medium increased the proportion of DHA and saturated fatty acids, including C12:0, C14:0 and C16:0, at the expense of monounsaturated fatty acids, including C16:1 and C18:1. Supplementation with 1 mg/L cerulenin resulted in the highest DHA yield of 2.4 mg/L upon co-expression of pfaE(DHA) from C. psychrerythraea.
[Mh] Termos MeSH primário: Alteromonadaceae/enzimologia
Proteínas de Bactérias/metabolismo
Ácidos Docosa-Hexaenoicos/biossíntese
Escherichia coli/metabolismo
Policetídeo Sintases/metabolismo
Transferases (Outros Grupos de Fosfato Substituídos)/metabolismo
[Mh] Termos MeSH secundário: Alteromonadaceae/genética
Sequência de Aminoácidos
Proteínas de Bactérias/genética
Vias Biossintéticas/genética
Cerulenina/farmacologia
Escherichia coli/efeitos dos fármacos
Escherichia coli/genética
Inibidores da Síntese de Ácidos Graxos/farmacologia
Regulação Bacteriana da Expressão Gênica
Engenharia Metabólica/métodos
Família Multigênica
Policetídeo Sintases/genética
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Água do Mar/microbiologia
Homologia de Sequência de Aminoácidos
Transferases (Outros Grupos de Fosfato Substituídos)/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Fatty Acid Synthesis Inhibitors); 0 (phosphopantetheinyl transferase); 17397-89-6 (Cerulenin); 25167-62-8 (Docosahexaenoic Acids); 79956-01-7 (Polyketide Synthases); EC 2.7.8.- (Transferases (Other Substituted Phosphate Groups))
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170816
[Lr] Data última revisão:
170816
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160921
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0162861


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[PMID]:27630308
[Au] Autor:Nishi K; Suzuki K; Sawamoto J; Tokizawa Y; Iwase Y; Yumita N; Ikeda T
[Ad] Endereço:Laboratory of Drug Metabolism and Pharmacotherapeutics, Department of Clinical Pharmacy, Yokohama University of Pharmacy, Yokohama, Japan k.nishi@hamayaku.ac.jp.
[Ti] Título:Inhibition of Fatty Acid Synthesis Induces Apoptosis of Human Pancreatic Cancer Cells.
[So] Source:Anticancer Res;36(9):4655-60, 2016 09.
[Is] ISSN:1791-7530
[Cp] País de publicação:Greece
[La] Idioma:eng
[Ab] Resumo:Cancer cells tend to have a high requirement for lipids, including fatty acids, cholesterol and triglyceride, because of their rapid proliferative rate compared to normal cells. In this study, we investigated the effects of inhibition of lipid synthesis on the proliferation and viability of human pancreatic cancer cells. Of the inhibitors of lipid synthesis that were tested, 5-(tetradecyloxy)-2-furoic acid (TOFA), which is an inhibitor of acetyl-CoA carboxylase, and the fatty acid synthase (FAS) inhibitors cerulenin and irgasan, significantly suppressed the proliferation of MiaPaCa-2 and AsPC-1 cells. Treatment of MiaPaCa-2 cells with these inhibitors significantly increased the number of apoptotic cells. In addition, TOFA increased caspase-3 activity and induced cleavage of poly (ADP-ribose) polymerase in MiaPaCa-2 cells. Moreover, addition of palmitate to MiaPaCa-2 cells treated with TOFA rescued cells from apoptotic cell death. These results suggest that TOFA induces apoptosis via depletion of fatty acids and that, among the various aspects of lipid metabolism, inhibition of fatty acid synthesis may be a notable target for the treatment of human pancreatic cancer cells.
[Mh] Termos MeSH primário: Apoptose
Ácido Graxo Sintases/antagonistas & inibidores
Ácidos Graxos/biossíntese
Neoplasias Pancreáticas/metabolismo
Neoplasias Pancreáticas/patologia
[Mh] Termos MeSH secundário: Anexina A5/química
Carbanilidas/química
Caspase 3/metabolismo
Linhagem Celular Tumoral
Proliferação Celular
Sobrevivência Celular
Cerulenina/química
Relação Dose-Resposta a Droga
Seres Humanos
Metabolismo dos Lipídeos
Lipídeos/química
Ácido Palmítico/química
Poli(ADP-Ribose) Polimerases/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Annexin A5); 0 (Carbanilides); 0 (Fatty Acids); 0 (Lipids); 17397-89-6 (Cerulenin); 2V16EO95H1 (Palmitic Acid); EC 2.3.1.85 (Fatty Acid Synthases); EC 2.4.2.30 (Poly(ADP-ribose) Polymerases); EC 3.4.22.- (CASP3 protein, human); EC 3.4.22.- (Caspase 3); I5ZZY3DC5G (cloflucarban)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170208
[Lr] Data última revisão:
170208
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160916
[St] Status:MEDLINE


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[PMID]:27595277
[Au] Autor:Noga MJ; Cerri M; Imholz N; Tulinski P; Sahin E; Bokinsky G
[Ad] Endereço:Department of Bionanoscience, Delft University of Technology, Kavli Institute of Nanoscience Delft , Lorentzweg 1, 2628CJ Delft, The Netherlands.
[Ti] Título:Mass-Spectrometry-Based Quantification of Protein-Bound Fatty Acid Synthesis Intermediates from Escherichia coli.
[So] Source:J Proteome Res;15(10):3617-3623, 2016 Oct 07.
[Is] ISSN:1535-3907
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The production of fatty acids from simple nutrients occurs via a complex biosynthetic pathway with dozens of intermediate compounds and multiple branch points. Despite its importance for microbial physiology and biotechnology, critical aspects of fatty acid biosynthesis, especially dynamics of in vivo regulation, remain poorly characterized. We have developed a liquid chromatography/mass spectroscopy (LC-MS) method for relative quantification of fatty acid synthesis intermediates in Escherichia coli, a model organism for studies of fatty acid metabolism. The acyl carrier protein, a vehicle for the substrates and intermediates of fatty acid synthesis, is extracted from E. coli, proteolytically digested, resolved using reverse-phase LC, and detected using electrospray ionization coupled with a tandem MS. Our method reliably resolves 21 intermediates of fatty acid synthesis, with an average relative standard deviation in ratios of individual acyl-ACP species to total ACP concentrations of 20%. We demonstrate that fast sampling and quenching of cells is essential to accurately characterize intracellular concentrations of ACP species. We apply our method to examine the rapid response of fatty acid metabolism to the antibiotic cerulenin. We anticipate that our method will enable the characterization of in vivo regulation and kinetics of microbial fatty acid synthesis at unprecedented detail and will improve integration of fatty acid synthesis into models of microbial metabolism.
[Mh] Termos MeSH primário: Proteínas de Bactérias/metabolismo
Escherichia coli/química
Ácidos Graxos/metabolismo
[Mh] Termos MeSH secundário: Proteína de Transporte de Acila/metabolismo
Vias Biossintéticas/efeitos dos fármacos
Proteínas de Transporte/metabolismo
Cerulenina/farmacologia
Ácidos Graxos/biossíntese
Espectrometria de Massas
Ligação Proteica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acyl Carrier Protein); 0 (Bacterial Proteins); 0 (Carrier Proteins); 0 (Fatty Acids); 17397-89-6 (Cerulenin)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170925
[Lr] Data última revisão:
170925
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160906
[St] Status:MEDLINE


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[PMID]:27314021
[Au] Autor:Lin WR; Lim SN; Yen TH; Alison MR
[Ad] Endereço:Department of Gastroenterology and Hepatology, Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Taoyuan 333, Taiwan.
[Ti] Título:The Influence of Bone Marrow-Secreted IL-10 in a Mouse Model of Cerulein-Induced Pancreatic Fibrosis.
[So] Source:Biomed Res Int;2016:4601532, 2016.
[Is] ISSN:2314-6141
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:This study aimed to understand the role of IL-10 secreted from bone marrow (BM) in a mouse model of pancreatic fibrosis. The severity of cerulein-induced inflammation, fibrosis, and the frequency of BM-derived myofibroblasts were evaluated in the pancreas of mice receiving either a wild-type (WT) BM or an IL-10 knockout (KO) BM transplantation. The area of collagen deposition increased significantly in the 3 weeks after cerulein cessation in mice with an IL-10 KO BM transplant (13.7 ± 0.6% and 18.4 ± 1.1%, p < 0.05), but no further increase was seen in WT BM recipients over this time. The percentage of BM-derived myofibroblasts also increased in the pancreas of the IL-10 KO BM recipients after cessation of cerulein (6.7 ± 1.1% and 11.9 ± 1.3%, p < 0.05), while this figure fell in WT BM recipients after cerulein withdrawal. Furthermore, macrophages were more numerous in the IL-10 KO BM recipients than the WT BM recipients after cerulein cessation (23.2 ± 2.3 versus 15.3 ± 1.7 per HPF, p < 0.05). In conclusion, the degree of fibrosis, inflammatory cell infiltration, and the number of BM-derived myofibroblasts were significantly different between IL-10 KO BM and WT BM transplanted mice, highlighting a likely role of IL-10 in pancreatitis.
[Mh] Termos MeSH primário: Fibrose/genética
Inflamação/genética
Interleucina-10/genética
Pâncreas/metabolismo
[Mh] Termos MeSH secundário: Animais
Transplante de Medula Óssea
Cerulenina/toxicidade
Colágeno/metabolismo
Fibrose/induzido quimicamente
Fibrose/patologia
Seres Humanos
Inflamação/induzido quimicamente
Inflamação/patologia
Camundongos
Camundongos Knockout
Miofibroblastos/efeitos dos fármacos
Miofibroblastos/patologia
Pâncreas/efeitos dos fármacos
Pâncreas/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (IL10 protein, mouse); 130068-27-8 (Interleukin-10); 17397-89-6 (Cerulenin); 9007-34-5 (Collagen)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160618
[St] Status:MEDLINE
[do] DOI:10.1155/2016/4601532


  9 / 462 MEDLINE  
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[PMID]:27305283
[Au] Autor:Sato YG; Suarez T; Saito T
[Ad] Endereço:a Graduate School of Science and Technology , Sophia University , Tokyo Japan.
[Ti] Título:Stalk cell differentiation without polyketides in the cellular slime mold.
[So] Source:Biosci Biotechnol Biochem;80(7):1368-74, 2016 Jul.
[Is] ISSN:1347-6947
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Polyketides induce prestalk cell differentiation in Dictyostelium. In the double-knockout mutant of the SteelyA and B polyketide synthases, most of the pstA cells-the major part of the prestalk cells-are lost, and we show by whole mount in situ hybridization that expression of prestalk genes is also reduced. Treatment of the double-knockout mutant with the PKS inhibitor cerulenin gave a further reduction, but some pstA cells still remained in the tip region, suggesting the existence of a polyketide-independent subtype of pstA cells. The double-knockout mutant and cerulenin-treated parental Ax2 cells form fruiting bodies with fragile, single-cell layered stalks after cerulenin treatment. Our results indicate that most pstA cells are induced by polyketides, but the pstA cells at the very tip of the slug are induced in some other way. In addition, a fruiting body with a single-cell layered, vacuolated stalk can form without polyketides.
[Mh] Termos MeSH primário: Diferenciação Celular/genética
Dictyostelium/genética
Policetídeo Sintases/genética
Policetídeos/metabolismo
Proteínas de Protozoários/genética
[Mh] Termos MeSH secundário: Cerulenina/farmacologia
Dictyostelium/efeitos dos fármacos
Dictyostelium/metabolismo
Dictyostelium/ultraestrutura
Inibidores Enzimáticos/farmacologia
Expressão Gênica
Técnicas de Inativação de Genes
Hibridização In Situ
Isoenzimas/antagonistas & inibidores
Isoenzimas/genética
Isoenzimas/metabolismo
Mutação
Policetídeo Sintases/antagonistas & inibidores
Policetídeo Sintases/metabolismo
Policetídeos/farmacologia
Proteínas de Protozoários/antagonistas & inibidores
Proteínas de Protozoários/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Enzyme Inhibitors); 0 (Isoenzymes); 0 (Polyketides); 0 (Protozoan Proteins); 17397-89-6 (Cerulenin); 79956-01-7 (Polyketide Synthases)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170118
[Lr] Data última revisão:
170118
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160616
[St] Status:MEDLINE
[do] DOI:10.1080/09168451.2016.1162087


  10 / 462 MEDLINE  
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[PMID]:27152772
[Au] Autor:Liu J; Mao X; Zhou W; Guarnieri MT
[Ad] Endereço:Institute for Food and Bioresource Engineering and Department of Energy and Resources Engineering, College of Engineering, Peking University, Beijing 100871, China. Electronic address: gjinliu@pku.edu.cn.
[Ti] Título:Simultaneous production of triacylglycerol and high-value carotenoids by the astaxanthin-producing oleaginous green microalga Chlorella zofingiensis.
[So] Source:Bioresour Technol;214:319-27, 2016 Aug.
[Is] ISSN:1873-2976
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The production of lipids and astaxanthin, a high-value carotenoid, by Chlorella zofingiensis was investigated under different culture conditions. Comparative analysis revealed a good correlation between triacylglycerol (TAG) and astaxanthin accumulation in C. zofingiensis. Stress conditions promoted cell size and weight and induced the accumulation of neutral lipids, especially TAG and astaxanthin, with a concomitant decrease in membrane lipids. The highest contents of TAG and astaxanthin achieved were 387 and 4.89mgg(-1) dry weight, respectively. A semi-continuous culture strategy was developed to optimize the TAG and astaxanthin productivities, which reached 297 and 3.3mgL(-1)day(-1), respectively. Additionally, astaxanthin accumulation was enhanced by inhibiting de novo fatty acid biosynthesis. In summary, our study represents a pioneering work of utilizing Chlorella for the integrated production of lipids and high-value products and C. zofingiensis has great potential to be a promising production strain and serve as an emerging oleaginous model alga.
[Mh] Termos MeSH primário: Biotecnologia/métodos
Carotenoides/metabolismo
Chlorella/metabolismo
Lipídeos/química
Microalgas/metabolismo
Triglicerídeos/metabolismo
[Mh] Termos MeSH secundário: Processos Autotróficos
Biomassa
Cerulenina/metabolismo
Chlorella/crescimento & desenvolvimento
Ácidos Graxos/biossíntese
Microalgas/crescimento & desenvolvimento
Processos Fototróficos
Estresse Fisiológico
Xantofilas/biossíntese
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fatty Acids); 0 (Lipids); 0 (Triglycerides); 0 (Xanthophylls); 17397-89-6 (Cerulenin); 36-88-4 (Carotenoids); 8XPW32PR7I (astaxanthine)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170130
[Lr] Data última revisão:
170130
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160507
[St] Status:MEDLINE



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