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[PMID]:27795309
[Au] Autor:Projahn M; Daehre K; Roesler U; Friese A
[Ad] Endereço:Institute for Animal Hygiene and Environmental Health, Freie Universität Berlin, Berlin, Germany michaela.projahn@fu-berlin.de.
[Ti] Título:Extended-Spectrum-Beta-Lactamase- and Plasmid-Encoded Cephamycinase-Producing Enterobacteria in the Broiler Hatchery as a Potential Mode of Pseudo-Vertical Transmission.
[So] Source:Appl Environ Microbiol;83(1), 2017 Jan 01.
[Is] ISSN:1098-5336
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Antimicrobial resistance through extended-spectrum beta-lactamases (ESBLs) and transferable (plasmid-encoded) cephamycinases (pAmpCs) represents an increasing problem in human and veterinary medicine. The presence of ESBL-/pAmpC-producing commensal enterobacteria in farm animals, such as broiler chickens, is considered one possible source of food contamination and could therefore also be relevant for human colonization. Studies on transmission routes along the broiler production chain showed that 1-day-old hatchlings are already affected. In this study, ESBL-/pAmpC-positive broiler parent flocks and their corresponding eggs, as well as various environmental and air samples from the hatchery, were analyzed. The eggs were investigated concerning ESBL-/pAmpC-producing enterobacteria on the outer eggshell surface (before/after disinfection), the inner eggshell surface, and the egg content. Isolates were analyzed concerning their species, their phylogroup in the case of Escherichia coli strains, the respective resistance genes, and the phenotypical antibiotic resistance. Of the tested eggs, 0.9% (n = 560) were contaminated on their outer shell surface. Further analyses using pulsed-field gel electrophoresis showed a relationship of these strains to those isolated from the corresponding parent flocks, which demonstrates a pseudo-vertical transfer of ESBL-/pAmpC-producing enterobacteria into the hatchery. Resistant enterobacteria were also found in environmental samples from the hatchery, such as dust or surfaces which could pose as a possible contamination source for the hatchlings. All 1-day-old chicks tested negative directly after hatching. The results show a possible entry of ESBL-/pAmpC-producing enterobacteria from the parent flocks into the hatchery; however, the impact of the hatchery on colonization of the hatchlings seems to be low. IMPORTANCE: ESBL-/pAmpC-producing enterobacteria occur frequently in broiler-fattening farms. Recent studies investigated the prevalence and possible transmission route of these bacteria in the broiler production chain. It seemed very likely that the hatcheries play an important role in transmission and/or contamination events. There are only few data on transmission investigations from a grandparent or parent flock to their offspring. However, reliable data on direct or indirect vertical transmission events in the hatchery are not available. Therefore, we conducted our study and intensively investigated the broiler hatching eggs from ESBL-/pAmpC-positive broiler parent flocks as well as the hatchlings and the environment of the hatchery.
[Mh] Termos MeSH primário: Cefamicinas/metabolismo
Infecções por Enterobacteriaceae/veterinária
Escherichia coli/genética
Escherichia/genética
Transmissão Vertical de Doença Infecciosa/veterinária
Doenças das Aves Domésticas/transmissão
beta-Lactamases/genética
[Mh] Termos MeSH secundário: Animais
Animais Domésticos
Galinhas/microbiologia
Farmacorresistência Bacteriana Múltipla/genética
Ovos/microbiologia
Infecções por Enterobacteriaceae/microbiologia
Infecções por Enterobacteriaceae/transmissão
Escherichia/efeitos dos fármacos
Escherichia/enzimologia
Escherichia/isolamento & purificação
Escherichia coli/efeitos dos fármacos
Escherichia coli/enzimologia
Escherichia coli/isolamento & purificação
Infecções por Escherichia coli/microbiologia
Infecções por Escherichia coli/transmissão
Infecções por Escherichia coli/veterinária
Fazendas
Seres Humanos
Plasmídeos
Doenças das Aves Domésticas/microbiologia
beta-Lactamases/biossíntese
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cephamycins); EC 3.5.2.6 (beta-Lactamases)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171102
[Lr] Data última revisão:
171102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161101
[St] Status:MEDLINE


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[PMID]:27394175
[Au] Autor:Liu D; Geng T; Wang Y; Ding L
[Ad] Endereço:Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing 210009, China; Nanjing Clinical Tech. Laboratories Inc., Nanjing 211100, China.
[Ti] Título:Pharmacokinetic profile of cefbuperazone in healthy Chinese volunteers after single and multiple drip intravenous infusion by HPLC-MS/MS.
[So] Source:J Pharm Biomed Anal;129:28-33, 2016 Sep 10.
[Is] ISSN:1873-264X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A selective and reproducible HPLC-MS/MS method was developed and fully validated for the determination of cefbuperazone in human plasma and urine. Samples were prepared using protein precipitation and separated on a Zorbax Eclipse Plus C18 column (2.1×50mm, 3.5µm). The API-4000 mass spectrometer was operated under multiple reaction monitoring mode (MRM) using the electrospray ionization technique. Linearity was achieved from 0.250 to 250µg/mL in plasma and 20.0-5000µg/mL in urine. The method was successfully applied to a pharmacokinetic study of cefbuperazone in healthy Chinese volunteers after drip intravenous infusion of 0.5, 1.0, 2.0g cefbuperazone sodium injection. Cefbuperazone reached a maximum concentration (Cmax) of 44.7±8.1µg/mL, 86.7±12.7µg/mL and 168±14µg/mL in 0.5, 1.0 and 2.0g dose groups respectively, at 60min after the start of infusion. The half-life (t1/2) was between 1.8-1.9h, and the elimination constant (kel) was between 0.36-0.39h(-1). The results proved that cefbuperazone showed linear pharmacokinetic profile in the dose range of 0.5-2.0g without gender difference. Drug accumulation was not observed. Cefbuperazone reached the maximum excretion rate in urine 2h after the start of infusion. About 60.0% of the administered drug was excreted via urine as unchanged form within 12h. The cumulative excretion of cefbuperazone after single drip intravenous infusion was proportional to the administered dose within the range from 0.5g to 2.0g.
[Mh] Termos MeSH primário: Grupo com Ancestrais do Continente Asiático
Cefamicinas/administração & dosagem
Cefamicinas/farmacocinética
Espectrometria de Massas em Tandem/métodos
[Mh] Termos MeSH secundário: Adulto
Cromatografia Líquida de Alta Pressão/métodos
Relação Dose-Resposta a Droga
Feminino
Seres Humanos
Infusões Intravenosas
Masculino
Distribuição Aleatória
Voluntários
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cephamycins); T0785J3X40 (cefbuperazone)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170808
[Lr] Data última revisão:
170808
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160711
[St] Status:MEDLINE


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[PMID]:26915052
[Au] Autor:Chon JW; Kim YJ; Kim HS; Kim DH; Kim H; Song KY; Sung K; Seo KH
[Ad] Endereço:Division of Microbiology, National Center for Toxicological Research, US Food and Drug Administration, Jefferson, AR 72079, USA.
[Ti] Título:Evaluation of cephamycins as supplements to selective agar for detecting Campylobacter spp. in chicken carcass rinses.
[So] Source:Int J Food Microbiol;223:75-8, 2016 Apr 16.
[Is] ISSN:1879-3460
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Although cefoperazone is the most commonly used antibiotic in Campylobacter-selective media, the distribution of cefoperazone-resistant bacteria such as extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli is increasing. Here we evaluated the potential of cephamycins for use as supplements to improve modified charcoal-cefoperazone-deoxycholate agar (mCCDA) by replacing cefoperazone with the same concentrations (32 mg/L) of cefotetan (modified charcoal-cefotetan-deoxycholate agar, mCCtDA) and cefoxitin (modified charcoal-cefoxitin-deoxycholate agar, mCCxDA). In chicken carcass rinse samples, the number of mCCDA plates detecting for Campylobacter (18/70, 26%) was significantly lower than that of mCCtDA (42/70, 60%) or mCCxDA plates (40/70, 57%). The number of mCCDA plates (70/70, 100%) that were contaminated with non-Campylobacter species was significantly higher than that of mCCtDA (20/70, 29%) or mCCxDA plates (21/70, 30%). The most common competing species identified using mCCDA was ESBL-producing E. coli, while Pseudomonas species frequently appeared on mCCtDA and mCCxDA.
[Mh] Termos MeSH primário: Ágar/química
Técnicas Bacteriológicas/métodos
Campylobacter/efeitos dos fármacos
Cefamicinas/farmacologia
Galinhas/microbiologia
Meios de Cultura/química
[Mh] Termos MeSH secundário: Animais
Antibacterianos/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Cephamycins); 0 (Culture Media); 9002-18-0 (Agar)
[Em] Mês de entrada:1608
[Cu] Atualização por classe:160304
[Lr] Data última revisão:
160304
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160226
[St] Status:MEDLINE


  4 / 1254 MEDLINE  
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[PMID]:26564965
[Au] Autor:Leite CA; Cavallieri AP; Baptista AS; Araujo ML
[Ad] Endereço:Department of Biochemistry and Technological Chemistry, Institute of Chemistry, UNESP-São Paulo State University, 14800-900 Araraquara, SP, Brazil carlaandrealeite@iq.unesp.br.
[Ti] Título:Dissociation of cephamycin C and clavulanic acid biosynthesis by 1,3-diaminopropane in Streptomyces clavuligerus.
[So] Source:FEMS Microbiol Lett;363(1):fnv215, 2016 Jan.
[Is] ISSN:1574-6968
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Streptomyces clavuligerus produces simultaneously cephamycin C (CephC) and clavulanic acid (CA). Adding 1,3-diaminopropane to culture medium stimulates production of beta-lactam antibiotics. However, there are no studies on the influence of this diamine on coordinated production of CephC and CA. This study indicates that 1,3-diaminopropane can dissociate CephC and CA productions. Results indicated that low diamine concentrations (below 1.25 g l(-1)) in culture medium increased CA production by 200%, but not that of CephC. Conversely, CephC production increased by 300% when 10 g l(-1) 1,3-diaminopropane was added to culture medium. Addition of just L-lysine (18.3 g l(-1)) to culture medium increased both biocompounds. On the other hand, while L-lysine plus 7.5 g l(-1) 1,3-diaminopropane increased volumetric production of CephC by 1100%, its impact on CA production was insignificant. The combined results suggest that extracellular concentration of 1,3-diaminopropane may trigger the dissociation of CephC and CA biosynthesis in S. clavuligerus.
[Mh] Termos MeSH primário: Vias Biossintéticas/efeitos dos fármacos
Cefamicinas/biossíntese
Ácido Clavulânico/biossíntese
Diaminas/metabolismo
Streptomyces/efeitos dos fármacos
Streptomyces/metabolismo
[Mh] Termos MeSH secundário: Meios de Cultura/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Cephamycins); 0 (Culture Media); 0 (Diamines); 23521W1S24 (Clavulanic Acid); CB3ISL56KG (trimethylenediamine); XA69811MWE (cephamycin C)
[Em] Mês de entrada:1609
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151114
[St] Status:MEDLINE


  5 / 1254 MEDLINE  
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[PMID]:26591539
[Au] Autor:Yin XP; Xie YM; Zhi YJ; Yang W; Wang ZF; Huo J
[Ti] Título:[Correlation analysis on combined medication with of Xiyanping injection in treatment of lung infection in real world].
[So] Source:Zhongguo Zhong Yao Za Zhi;40(12):2440-4, 2015 Jun.
[Is] ISSN:1001-5302
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:To analyze the regularity in combined medication with Xiyanping injection (Xiyanping for short) in the real world by as- sociation rules. Totally 5 822 patients using Xiyanping injection was collected from the 18 Class III Grade I hospitals nationwide to study the combined medication information of the patient with lung infection and make the analysis by using association rules and Apriori. According to the results, major drugs combined with Xiyanping in treatment of lung infection included compound amino acid, inosine, coenzyme A, cytidine triphosphate, vitamin C. Common drugs combined with Xiyanping can be divided into 5 categories: nutrition support therapy (vitamin C, compound amino acid) , coenzymes (coenzyme A, cytidine triphosphate, inosine), expectorants and antiasthmatics (ambroxol, salbutamol, doxofylline), hormones (dexamethasone, budesonide), antibiotics (mainly cefminox). The main combined medicines mostly conformed to the regularity for drugs treating lung infection. In addition, there were two most common medical combination models: the model for Xiyanping combined a single medicine is Xiyanping + nutrition support therapy, while the model for Xiyanping combined two or more than two medicines is Xiyanping + nutrition support therapy + coenzyme. Pharmacologically, Xiyanping is mostly combined with western medicines with similar pharmacological effects to substitute or supplement the antibiotic effect in treating lung infection. However, further studies shall be conducted for the safety and rationality of the combined medication based on clinical practices, in order to provide reference for clinical medication.
[Mh] Termos MeSH primário: Quimioterapia Combinada
Medicamentos de Ervas Chinesas/administração & dosagem
Pneumopatias/tratamento farmacológico
[Mh] Termos MeSH secundário: Adulto
Antibacterianos/administração & dosagem
Ácido Ascórbico/administração & dosagem
Cefamicinas/administração & dosagem
Feminino
Sistemas de Informação Hospitalar
Seres Humanos
Masculino
Meia-Idade
Adulto Jovem
[Pt] Tipo de publicação:ENGLISH ABSTRACT; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Cephamycins); 0 (Drugs, Chinese Herbal); PQ6CK8PD0R (Ascorbic Acid); PW08Y13465 (cefminox)
[Em] Mês de entrada:1512
[Cu] Atualização por classe:151120
[Lr] Data última revisão:
151120
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151124
[St] Status:MEDLINE


  6 / 1254 MEDLINE  
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[PMID]:26100709
[Au] Autor:Rodríguez-Baño J
[Ad] Endereço:Unidad Clínica de Enfermedades Infecciosas, Microbiología y Medicina Preventiva, Hospitales Universitarios Virgen Macarena and Virgen del Rocío, Seville, Spain Departamento de Medicina, Universidad de Sevilla, Seville, Spain jesusrb@us.es.
[Ti] Título:The times they are a-changin': carbapenems for extended-spectrum-ß-lactamase-producing bacteria.
[So] Source:Antimicrob Agents Chemother;59(9):5095-6, 2015 Sep.
[Is] ISSN:1098-6596
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Several antimicrobial agents are being investigated as alternatives to carbapenems in the treatment of infections caused by ESBL-producing Enterobacteriaceae, which may be useful in avoiding overuse of carbapenems in the context of recent global spread of carbapenem-resistant Enterobacteriaceae. The most promising candidates for invasive infections so far are ß-lactam/ß-lactamase inhibitor combinations and cephamycins.
[Mh] Termos MeSH primário: Carbapenêmicos/farmacologia
Enterobacteriaceae/efeitos dos fármacos
beta-Lactamases/metabolismo
[Mh] Termos MeSH secundário: Antibacterianos/farmacologia
Cefamicinas/farmacologia
Enterobacteriaceae/enzimologia
Testes de Sensibilidade Microbiana
Inibidores de beta-Lactamases/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Carbapenems); 0 (Cephamycins); 0 (beta-Lactamase Inhibitors); EC 3.5.2.6 (beta-Lactamases)
[Em] Mês de entrada:1605
[Cu] Atualização por classe:160301
[Lr] Data última revisão:
160301
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150624
[St] Status:MEDLINE
[do] DOI:10.1128/AAC.01333-15


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[PMID]:25068396
[Au] Autor:Rajenderan S; Balaji V; Anandan S; Sahni RD; Tansarli GS; Falagas ME
[Ad] Endereço:Christian Medical College, Vellore, Tamil Nadu, South India.
[Ti] Título:Determination of MIC distribution of arbekacin, cefminox, fosfomycin, biapenem and other antibiotics against gram-negative clinical isolates in South India: a prospective study.
[So] Source:PLoS One;9(7):e103253, 2014.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVES: To determine the in vitro activity of antibiotics, including arbekacin, cefminox, fosfomycin and biapenem which are all still unavailable in India, against Gram-negative clinical isolates. METHODS: We prospectively collected and tested all consecutive isolates of Escherichia coli, Klebsiella spp., Pseudomonas aeruginosa and Acinetobacter spp. from blood, urine and sputum samples between March and November 2012. The minimum inhibition concentration (MIC) of 16 antibiotics was determined by the broth micro-dilution method. RESULTS: Overall 925 isolates were included; 211 E. coli, 207 Klebsiella spp., 153 P. aeruginosa, and 354 Acinetobacter spp. The MIC50 and MIC90 were high for cefminox, biapenem and arbekacin for all pathogens but interpretative criteria were not available. The MIC50 was categorized as susceptible for a couple of antibiotics, including piperacillin/tazobactam, carbapenems and amikacin, for E. coli, Klebsiella spp. and P. aeruginosa. However, for Acinetobacter spp., the MIC50 was categorized as susceptible only for colistin. On the other hand, fosfomycin was the only antibiotic that inhibited 90% of E. coli and Klebsiella spp. isolates, while 90% of P. aeruginosa isolates were inhibited only by colistin. Finally, 90% of Acinetobacter spp. isolates were not inhibited by any antibiotic tested. CONCLUSION: Fosfomycin and colistin might be promising antibiotics for the treatment of infections due to E. coli or Klebsiella spp. and P. aeruginosa, respectively, in India; however, clinical trials should first corroborate the in vitro findings. The activity of tigecycline should be evaluated, as this is commonly used as last-resort option for the treatment of multidrug-resistant Acinetobacter infections.
[Mh] Termos MeSH primário: Cefamicinas/farmacologia
Dibecacina/análogos & derivados
Fosfomicina/farmacologia
Bactérias Gram-Negativas/efeitos dos fármacos
Tienamicinas/farmacologia
[Mh] Termos MeSH secundário: Acinetobacter/efeitos dos fármacos
Acinetobacter/fisiologia
Antibacterianos/farmacologia
Dibecacina/farmacologia
Farmacorresistência Bacteriana Múltipla
Escherichia coli/efeitos dos fármacos
Escherichia coli/fisiologia
Bactérias Gram-Negativas/fisiologia
Infecções por Bactérias Gram-Negativas/microbiologia
Seres Humanos
Índia
Klebsiella/efeitos dos fármacos
Klebsiella/fisiologia
Testes de Sensibilidade Microbiana/métodos
Estudos Prospectivos
Pseudomonas aeruginosa/efeitos dos fármacos
Pseudomonas aeruginosa/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Cephamycins); 0 (Thienamycins); 2N81MY12TE (Fosfomycin); 45ZFO9E525 (Dibekacin); G7V6SLI20L (arbekacin); PW08Y13465 (cefminox); YR5U3L9ZH1 (biapenem)
[Em] Mês de entrada:1512
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140729
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0103253


  8 / 1254 MEDLINE  
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[PMID]:24975573
[Au] Autor:Martínez-Burgo Y; Álvarez-Álvarez R; Pérez-Redondo R; Liras P
[Ad] Endereço:Microbiology Section, Department of Molecular Biology, Faculty of Biology and Environmental Sciences, Vegazana Campus, University of León, León 24071, Spain.
[Ti] Título:Heterologous expression of Streptomyces clavuligerus ATCC 27064 cephamycin C gene cluster.
[So] Source:J Biotechnol;186:21-9, 2014 Sep 30.
[Is] ISSN:1873-4863
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The Streptomyces clavuligerus cephamycin C gene cluster has been subcloned in a SuperCos-derived cosmid and introduced in Streptomyces flavogriseus ATCC 33331, Streptomyces coelicolor M1146 and Streptomyces albus J1074. The exconjugant strains were supplemented with an additional copy of the S. clavuligerus cephamycin regulatory activator gene, ccaRC, expressed from the constitutive Pfur promoter. Only S. flavogriseus-derived exconjugants produced a compound active against Escherichia coli ESS22-31 that was characterized by HPLC-MS as cephamycin C. The presence of an additional ccaR copy resulted in about 40-fold increase in cephamycin C production. Optimal heterologous cephamycin C production was in the order of 9% in relation to that of S. clavuligerus ATCC 27064. RT-qPCR studies indicated that ccaRC expression in S. flavogriseus::[SCos-CF] was 7% of that in S. clavuligerus and increased to 47% when supplemented with a copy of Pfur-ccaR. The effect on cephamycin biosynthesis gene expression was thus improved but not in an uniform manner for every gene. In heterologous strains, integration of the cephamycin cluster results in a ccaR-independent increased resistance to penicillin, cephalosporin and cefoxitin, what corresponds well to the strong expression of the pcbR and pbpA genes in S. flavogriseus-derived strains.
[Mh] Termos MeSH primário: Antibacterianos/metabolismo
Cefamicinas/metabolismo
Família Multigênica/genética
Streptomyces/genética
Streptomyces/metabolismo
[Mh] Termos MeSH secundário: Antibacterianos/farmacologia
Bactérias/efeitos dos fármacos
Cefamicinas/farmacologia
Clonagem Molecular
DNA Recombinante
Técnicas de Transferência de Genes
Testes de Sensibilidade Microbiana
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Cephamycins); 0 (DNA, Recombinant); XA69811MWE (cephamycin C)
[Em] Mês de entrada:1506
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140701
[St] Status:MEDLINE


  9 / 1254 MEDLINE  
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[PMID]:24691683
[Au] Autor:Pilmis B; Parize P; Zahar JR; Lortholary O
[Ad] Endereço:Service des Maladies Infectieuses et Tropicales, Institut Imagine, APHP, Centre d'Infectiologie Necker-Pasteur, Université Paris-Descartes, Hôpital Necker-Enfants Malades, 149 rue de Sèvres, 75015, Paris, France, bpilmis@gmail.com.
[Ti] Título:Alternatives to carbapenems for infections caused by ESBL-producing Enterobacteriaceae.
[So] Source:Eur J Clin Microbiol Infect Dis;33(8):1263-5, 2014 Aug.
[Is] ISSN:1435-4373
[Cp] País de publicação:Germany
[La] Idioma:eng
[Mh] Termos MeSH primário: Antibacterianos/uso terapêutico
Carbapenêmicos/uso terapêutico
Cefamicinas/uso terapêutico
Infecções por Enterobacteriaceae/tratamento farmacológico
Enterobacteriaceae/enzimologia
Sepse/tratamento farmacológico
Infecções Urinárias/tratamento farmacológico
[Mh] Termos MeSH secundário: Adulto
Antibacterianos/farmacologia
Proteínas de Bactérias/biossíntese
Carbapenêmicos/farmacologia
Cefamicinas/farmacologia
Criança
Pré-Escolar
Infecções por Enterobacteriaceae/microbiologia
Feminino
Seres Humanos
Masculino
Estudos Retrospectivos
Sepse/microbiologia
Infecções Urinárias/microbiologia
Resistência beta-Lactâmica
beta-Lactamases/biossíntese
[Pt] Tipo de publicação:LETTER
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Bacterial Proteins); 0 (Carbapenems); 0 (Cephamycins); EC 3.5.2.6 (beta-Lactamases)
[Em] Mês de entrada:1502
[Cu] Atualização por classe:171013
[Lr] Data última revisão:
171013
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140403
[St] Status:MEDLINE
[do] DOI:10.1007/s10096-014-2094-y


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[PMID]:24450885
[Au] Autor:Alvarez-Álvarez R; Rodríguez-García A; Santamarta I; Pérez-Redondo R; Prieto-Domínguez A; Martínez-Burgo Y; Liras P
[Ad] Endereço:Área de Microbiología, Departamento de Biología Molecular, Facultad de CC, Biológicas y Ambientales, Universidad de León, León, Spain; Instituto de Biotecnología de Léon (INBIOTEC), Parque Científico de León, León, Spain.
[Ti] Título:Transcriptomic analysis of Streptomyces clavuligerus ΔccaR::tsr: effects of the cephamycin C-clavulanic acid cluster regulator CcaR on global regulation.
[So] Source:Microb Biotechnol;7(3):221-31, 2014 May.
[Is] ISSN:1751-7915
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Streptomyces clavuligerus ATCC 27064 and S. clavuligerus ΔccaR::tsr cultures were grown in asparagine-starch medium, and samples were taken in the exponential and stationary growth phases. Transcriptomic analysis showed that the expression of 186 genes was altered in the ccaR-deleted mutant. These genes belong to the cephamycin C gene cluster, clavulanic acid gene cluster, clavams, holomycin, differentiation, carbon, nitrogen, amino acids or phosphate metabolism and energy production. All the clavulanic acid biosynthesis genes showed Mc values in the order of -4.23. The blip gene-encoding a ß-lactamase inhibitory protein was also controlled by the cephamycin C-clavulanic acid cluster regulator (Mc -2.54). The expression of the cephamycin C biosynthesis genes was greatly reduced in the mutant (Mc values up to -7.1), while the genes involved in putative ß-lactam resistance were less affected (Mc average -0.88). Genes for holomycin biosynthesis were upregulated. In addition, the lack of clavulanic acid and cephamycin production negatively affected the expression of genes for the clavulanic acid precursor arginine and of miscellaneous genes involved in nitrogen metabolism (amtB, glnB, glnA3, glnA2, glnA1). The transcriptomic results were validated by quantative reverse transcription polymerase chain reaction and luciferase assay of luxAB-coupled promoters. Transcriptomic analysis of the homologous genes of S. coelicolor validated the results obtained for S. clavuligerus primary metabolism genes.
[Mh] Termos MeSH primário: Vias Biossintéticas/genética
Cefamicinas/metabolismo
Ácido Clavulânico/metabolismo
Regulação Bacteriana da Expressão Gênica
Família Multigênica
Streptomyces/metabolismo
Fatores de Transcrição/genética
[Mh] Termos MeSH secundário: Meios de Cultura/química
Deleção de Genes
Perfilação da Expressão Gênica
Genes Reporter
Luciferases/análise
Luciferases/genética
Reação em Cadeia da Polimerase em Tempo Real
Streptomyces/genética
Streptomyces/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Cephamycins); 0 (Culture Media); 0 (Transcription Factors); 23521W1S24 (Clavulanic Acid); EC 1.13.12.- (Luciferases); XA69811MWE (cephamycin C)
[Em] Mês de entrada:1411
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140124
[St] Status:MEDLINE
[do] DOI:10.1111/1751-7915.12109



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