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[PMID]:29187950
[Au] Autor:Ibrahim OMA; Bilal NE; Osman OF; Magzoub MA
[Ad] Endereço:Department of Microbiology, Faculty of Medicine and Health Sciences, University of El-imam El-mahdi P.O Box 209, Kosti City, Sudan.
[Ti] Título:Assessment of methicillin resistant Aureus detection methods: analytical comparative study.
[So] Source:Pan Afr Med J;27:281, 2017.
[Is] ISSN:1937-8688
[Cp] País de publicação:Uganda
[La] Idioma:eng
[Ab] Resumo:Introduction: The heterogeneous expression of methicillin resistance in (MRSA) affects the efficiency of tests available to detect it. The objective of this study was to assess four phenotypic tests used to detect MRSA. Methods: This is an analytical comparative study conducted among sudanese patients during period from May 2012 to July 2014, strains were isolated and identified by conventional methods, and then confirmed by PCR detection of coagulase gene. PCR detection of mecA gene was used as a gold standard to assess oxacillin resistance screen agar base (ORSAB), oxacillin disc, cefoxitin disc (at different temperatures and incubation periods) and MRSA-latex agglutination test. ATCC 25923 was used as control. Sensitivity and specificity were calculated. Results: MRSA- latex agglutination was the most accurate test; it showed 100% of both sensitivity and specificity, followed by cefoxitin disc with sensitivity of 98.48% and specificity of 100%. However, both of oxacillin disc and oxacillin resistance screen agar base showed less accurate results, and were affected by incubation periods. Oxacillin disc after 24 h incubation both at 30°C and 35°C showed sensitivity and specificity values of 87.88% and 96.23%, respectively. However, after 48h incubation the test at 30°C showed sensitivity and specificity values of 89.39%, and 94.34%, respectively. At 35°C (48h) it showed values of 89.39%, 92.45% respectively. Specificity of ORSAB was more than oxacillin disc at 35°C after 24h incubation 98.11% and 96.23%, respectively. Conclusion: MRSA- latex agglutination and cefoxitin disc diffusion tests are recommended for routine detection of MRSA.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Proteínas de Bactérias/genética
Staphylococcus aureus Resistente à Meticilina/isolamento & purificação
Proteínas de Ligação às Penicilinas/genética
Infecções Estafilocócicas/diagnóstico
[Mh] Termos MeSH secundário: Cefoxitina/farmacologia
Farmacorresistência Bacteriana
Seres Humanos
Testes de Fixação do Látex
Staphylococcus aureus Resistente à Meticilina/efeitos dos fármacos
Testes de Sensibilidade Microbiana
Oxacilina/farmacologia
Reação em Cadeia da Polimerase/métodos
Sensibilidade e Especificidade
Infecções Estafilocócicas/dietoterapia
Infecções Estafilocócicas/microbiologia
Temperatura Ambiente
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Bacterial Proteins); 0 (Penicillin-Binding Proteins); 0 (mecA protein, Staphylococcus aureus); 6OEV9DX57Y (Cefoxitin); UH95VD7V76 (Oxacillin)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171219
[Lr] Data última revisão:
171219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171201
[St] Status:MEDLINE
[do] DOI:10.11604/pamj.2017.27.281.9016


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[PMID]:28820112
[Au] Autor:Abdalhamid B; Albunayan S; Shaikh A; Elhadi N; Aljindan R
[Ad] Endereço:1​Department of Pathology and Laboratory Medicine, King Fahad Specialist Hospital, PO Box 15215, Dammam, Saudi Arabia.
[Ti] Título:Prevalence study of plasmid-mediated AmpC ß-lactamases in Enterobacteriaceae lacking inducible ampC from Saudi hospitals.
[So] Source:J Med Microbiol;66(9):1286-1290, 2017 Sep.
[Is] ISSN:1473-5644
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:PURPOSE: Enterobacteriaceae encoding plasmid-mediated AmpC (pAmpC) ß-lactamases confer resistance to the third generation cephalosporins. pAmpC association with extended spectrum ß-lactamases (ESBLs), plasmid-mediated quinolone resistance (PMQR) and aminoglycoside modifying enzymes (AMEs) is well documented. There are limited data regarding the epidemiology and clinical significance of pAmpC in Saudi Arabia. This study aimed to determine the prevalence of pAmpC and its coexistence with ESBLs, PMQR and AMEs in Escherichia coli, Klebsiella pneumoniae and Proteus mirabilis isolates in Saudi hospitals from January to December 2015. METHODOLOGY: The VITEK 2 system was used for organism identification and susceptibility testing. PCR and sequencing were used to detect pAmpC, ESBL, AME and PMQR genes. RESULTS: Out of 3625 isolates of E. coli, K. pneumoniae and P. mirabilis, 200 cefoxitin-resistant isolates were identified, making the prevalence of cefoxitin resistance 5.5 % (200/3625). CMY-2 and DHA were detected in 24 and 12 isolates, respectively. The prevalence of pAmpC was 1 % (36/3625). In several isolates, pAmpC ß-lactamases were associated with PMQR genes including aac(6')-Ib-cr and qnrB and/or with AMEs including aacA4, aacC2, aadA1, aphA6, armA and rmtB genes. No ESBLs were detected in pAmpC ß-lactamase-harbouring isolates. CONCLUSIONS: To our knowledge, this is the first study determining the prevalence of pAmpC ß-lactamases and their association with PMQR and/or AME genes in Saudi Arabia and the Gulf States. CMY-2 is the most prevalent pAmpC ß-lactamase in this study. These data emphasize the importance of surveillance studies and implementation of antimicrobial stewardship programmes to reduce infections caused by such resistant organisms.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Proteínas de Bactérias/genética
Cefoxitina/farmacologia
Escherichia coli/genética
Klebsiella pneumoniae/genética
Proteus mirabilis/genética
beta-Lactamases/genética
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
DNA Bacteriano/genética
Escherichia coli/efeitos dos fármacos
Escherichia coli/isolamento & purificação
Feminino
Seres Humanos
Klebsiella pneumoniae/efeitos dos fármacos
Klebsiella pneumoniae/isolamento & purificação
Masculino
Testes de Sensibilidade Microbiana
Plasmídeos/genética
Prevalência
Proteus mirabilis/efeitos dos fármacos
Proteus mirabilis/isolamento & purificação
Arábia Saudita
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Bacterial Proteins); 0 (DNA, Bacterial); 6OEV9DX57Y (Cefoxitin); EC 3.5.2.6 (AmpC beta-lactamases); EC 3.5.2.6 (beta-Lactamases)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170819
[St] Status:MEDLINE
[do] DOI:10.1099/jmm.0.000504


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[PMID]:28530888
[Au] Autor:Månsson E; Hellmark B; Stegger M; Skytt Andersen P; Sundqvist M; Söderquist B
[Ad] Endereço:2​Centre for Clinical Research, Hospital of Västmanland Västerås, SE-721 89 Västerås, Sweden 1​School of Medical Sciences, Faculty of Medicine and Health, Örebro University, SE-701 82 Örebro, Sweden.
[Ti] Título:Genomic relatedness of Staphylococcus pettenkoferi isolates of different origins.
[So] Source:J Med Microbiol;66(5):601-608, 2017 May.
[Is] ISSN:1473-5644
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:PURPOSE: The aim of the study was to characterize clinical and environmental Staphylococcus pettenkoferi isolates with regard to genomic diversity and antibiotic susceptibility pattern. Repetitive-sequence-based PCR and core genome phylogenetic analysis of whole-genome sequencing (WGS) data verified the presence of distinct clades comprising closely related S. pettenkoferi isolates from different geographical locations and origins. METHODOLOGY: Phylogenetic relationships between 25 S. pettenkoferi isolates collected from blood cultures and intra-operative air sampling were determined by repetitive-sequence-based PCR typing and analysis of ~157 000 SNPs identified in the core genome after WGS. Antibiotic susceptibility testing and tests for biofilm production (microtitre plate assay) were performed. RESULTS: Repetitive-sequence-based PCR as well as WGS data demonstrated the close relatedness of clinically significant blood culture isolates to probable contaminants, as well as to environmental isolates. Antibiotic-susceptibility testing demonstrated a low level of antimicrobial resistance. The mecA gene was present in two cefoxitin-resistant isolates. No isolates were found to produce biofilm. CONCLUSION: Close genomic relatedness of S. pettenkoferi isolates from different geographical locations and origins were found within clades, but with substantial genomic difference between the two major clades. The ecological niche of S. pettenkoferi remains unconfirmed, but the presence of S. pettenkoferi in the air of the operating field favours the suggestion of a role in skin flora. Identification of S. pettenkoferi in clinical samples should, in a majority of cases, most likely be regarded as a probable contamination, and its role as a possible pathogen in immunocompromised hosts remains to be clarified.
[Mh] Termos MeSH primário: Infecções Estafilocócicas/microbiologia
Staphylococcus/classificação
Staphylococcus/genética
[Mh] Termos MeSH secundário: Antibacterianos/farmacologia
Proteínas de Bactérias/genética
Biofilmes/crescimento & desenvolvimento
Cefoxitina/farmacologia
Farmacorresistência Bacteriana/genética
Genoma Bacteriano
Seres Humanos
Testes de Sensibilidade Microbiana
Filogenia
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Infecções Estafilocócicas/sangue
Staphylococcus/efeitos dos fármacos
Staphylococcus/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Bacterial Proteins); 0 (RNA, Ribosomal, 16S); 6OEV9DX57Y (Cefoxitin)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170614
[Lr] Data última revisão:
170614
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170523
[St] Status:MEDLINE
[do] DOI:10.1099/jmm.0.000472


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[PMID]:28334649
[Au] Autor:Chen X; Li Y; Zhang Y; Yang J; Bian L
[Ad] Endereço:College of Life Science, Northwest University, Xi'an 710069, PR China.
[Ti] Título:Binding of TEM-1 beta-lactamase to beta-lactam antibiotics by frontal affinity chromatography.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;1051:75-83, 2017 Apr 15.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:TEM-1 beta-lactamases can accurately catalyze the hydrolysis of the beta-lactam rings in beta-lactam antibiotics, which make beta-lactam antibiotics lose its activity, and the prerequisite for the hydrolysis procedure in the binding interaction of TEM-1 beta-lactamases with beta-lactam antibiotics is the beta-lactam rings in beta-lactam antibiotics. Therefore, the binding of TEM-1 beta-lactamase to three beta-lactam antibiotics including penicillin G, cefalexin as well as cefoxitin was explored here by frontal affinity chromatography in combination with fluorescence spectra, adsorption and thermodynamic data in the temperature range of 278-288K under simulated physiological conditions. The results showed that all the binding of TEM-1 beta-lactamase to the three antibiotics were spontaneously exothermic processes with the binding constants of 8.718×10 , 6.624×10 and 2.244×10 (mol/L), respectively at 288K. All the TEM-1 beta-lactamases were immobilized on the surface of the stationary phase in the mode of monolayer and there existed only one type of binding sites on them. Each TEM-1 beta-lactamase bound with only one beta-lactam antibiotic and hydrogen bond interaction and Van der Waals force were the main forces between them. This work provided an insight into the binding interactions between TEM-1 beta-lactamases and beta-lactam antibiotics, which may be beneficial for the designing and developing of new substrates resistant to TEM-1 beta-lactamases.
[Mh] Termos MeSH primário: Antibacterianos/metabolismo
Cromatografia de Afinidade/métodos
Bactérias Gram-Negativas/enzimologia
beta-Lactamases/metabolismo
beta-Lactamas/metabolismo
[Mh] Termos MeSH secundário: Cefoxitina/metabolismo
Cefalexina/metabolismo
Enzimas Imobilizadas/metabolismo
Bactérias Gram-Negativas/metabolismo
Penicilina G/metabolismo
Ligação Proteica
Termodinâmica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Enzymes, Immobilized); 0 (beta-Lactams); 6OEV9DX57Y (Cefoxitin); EC 3.5.2.6 (beta-Lactamases); EC 3.5.2.6 (beta-lactamase TEM-1); OBN7UDS42Y (Cephalexin); Q42T66VG0C (Penicillin G)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170601
[Lr] Data última revisão:
170601
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170324
[St] Status:MEDLINE


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[PMID]:28137797
[Au] Autor:Edirmanasinghe R; Finley R; Parmley EJ; Avery BP; Carson C; Bekal S; Golding G; Mulvey MR
[Ad] Endereço:Department of Medical Microbiology, University of Manitoba, Winnipeg, MB, Canada.
[Ti] Título:A Whole-Genome Sequencing Approach To Study Cefoxitin-Resistant Salmonella enterica Serovar Heidelberg Isolates from Various Sources.
[So] Source:Antimicrob Agents Chemother;61(4), 2017 Apr.
[Is] ISSN:1098-6596
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:This study characterized cefoxitin-resistant and -susceptible serovar Heidelberg strains from humans, abattoir poultry, and retail poultry to assess the molecular relationships of isolates from these sources in Québec in 2012. Isolates were collected as part of the Canadian Integrated Program for Antimicrobial Resistance Surveillance (CIPARS). All isolates were subjected to antimicrobial susceptibility testing, PCR for CMY-2, pulsed-field gel electrophoresis (PFGE), and whole-genome sequencing (WGS). A total of 113 Heidelberg isolates from humans ( = 51), abattoir poultry ( = 18), and retail poultry ( = 44) were studied. All cefoxitin-resistant isolates ( = 65) were also resistant to amoxicillin-clavulanic acid, ampicillin, ceftiofur, and ceftriaxone, and all contained the CMY-2 gene. PFGE analysis showed that 111/113 (98.2%) isolates clustered together with ≥90% similarity. Core genome analysis using WGS identified 13 small clusters of isolates with 0 to 4 single nucleotide variations (SNVs), consisting of cefoxitin-resistant and -susceptible human, abattoir poultry, and retail poultry isolates. CMY-2 plasmids from cefoxitin-resistant isolates all belonged to incompatibility group I1. Analysis of IncI1 plasmid sequences revealed high identity (95 to 99%) to a previously described plasmid (pCVM29188_101) found in Kentucky. When compared to pCVM29188_101, all sequenced cefoxitin-resistant isolates were found to carry 1 of 10 possible variant plasmids. Transmission of Heidelberg may be occurring between human, abattoir poultry, and retail poultry sources, and transmission of a common CMY-2 plasmid may be occurring among Heidelberg strains with variable genetic backgrounds.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Cefoxitina/farmacologia
Farmacorresistência Bacteriana Múltipla/genética
Genoma Bacteriano
Carne/microbiologia
Salmonella enterica/efeitos dos fármacos
beta-Lactamases/genética
[Mh] Termos MeSH secundário: Combinação Amoxicilina e Clavulanato de Potássio/farmacologia
Ampicilina/farmacologia
Animais
Canadá/epidemiologia
Ceftriaxona/farmacologia
Cefalosporinas/farmacologia
Galinhas
Eletroforese em Gel de Campo Pulsado
Monitoramento Epidemiológico
Expressão Gênica
Sequenciamento de Nucleotídeos em Larga Escala
Seres Humanos
Testes de Sensibilidade Microbiana
Filogenia
Polimorfismo de Nucleotídeo Único
Infecções por Salmonella/epidemiologia
Infecções por Salmonella/microbiologia
Infecções por Salmonella/transmissão
Salmonella enterica/classificação
Salmonella enterica/genética
Salmonella enterica/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Cephalosporins); 6OEV9DX57Y (Cefoxitin); 74469-00-4 (Amoxicillin-Potassium Clavulanate Combination); 75J73V1629 (Ceftriaxone); 7C782967RD (Ampicillin); 83JL932I1C (ceftiofur); EC 3.5.2.- (beta-lactamase CMY-2); EC 3.5.2.6 (beta-Lactamases)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170919
[Lr] Data última revisão:
170919
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170201
[St] Status:MEDLINE


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[PMID]:28137277
[Au] Autor:Fowoyo PT; Ogunbanwo ST
[Ad] Endereço:Biosciences Department, Salem University, P.M.B. 1060, Lokoja, Kogi State, Nigeria. seunpt@yahoo.com.
[Ti] Título:Antimicrobial resistance in coagulase-negative staphylococci from Nigerian traditional fermented foods.
[So] Source:Ann Clin Microbiol Antimicrob;16(1):4, 2017 Jan 31.
[Is] ISSN:1476-0711
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Coagulase-negative staphylococci have become increasingly recognized as the etiological agent of some infections. A significant characteristic of coagulase-negative staphylococci especially strains isolated from animals and clinical samples is their resistance to routinely used antibiotics although, resistant strains isolated from fermented foods have not been fully reported. METHODS: A total of two hundred and fifty-five CoNS isolates were subjected to antimicrobial susceptibility test using the disc diffusion technique. The minimum inhibitory concentration of the isolates to the tested antibiotics was determined using the microbroth dilution method. Methicillin resistant strains were confirmed by detection of methicillin resistant genes (mecA) and also employing cefoxitin screening test. RESULTS: The isolates were confirmed to be methicillin resistant by the detection of mecA genes and the cefoxitin screening test. The isolates demonstrated appreciable resistance to ampicillin (86.7%), sulfomethoxazole-trimethoprim (74.9%), amoxicillin-clavulanic acid (52.5%) and oxacillin (35.7%). Methicillin resistance was exhibited by 13 out of the 255 isolates although no mecA gene was detected. It was also observed that the methicillin resistant isolates were prevalent in these traditional foods; iru, kindirmo, nono and wara. CONCLUSION: This study has ameliorated the incidence of multiple antibiotic resistant coagulase-negative staphylococci in Nigerian fermented foods and if not tackled adequately might lead to horizontal transfer of antibiotic resistance from food to man.
[Mh] Termos MeSH primário: Adenosina/análogos & derivados
Anti-Infecciosos/farmacologia
Microbiologia de Alimentos
Resistência a Meticilina/genética
Infecções Estafilocócicas/microbiologia
Staphylococcus/efeitos dos fármacos
[Mh] Termos MeSH secundário: Adenosina/genética
Ampicilina/farmacologia
Animais
Proteínas de Bactérias/genética
Cefoxitina/farmacologia
Coagulase/genética
Fermentação
Seres Humanos
Meticilina/farmacologia
Testes de Sensibilidade Microbiana
Nigéria
Oxacilina/farmacologia
Fenótipo
Análise de Sequência de DNA
Staphylococcus/genética
Staphylococcus/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Infective Agents); 0 (Bacterial Proteins); 0 (Coagulase); 35788-27-3 (5'-N-methylcarboxamideadenosine); 6OEV9DX57Y (Cefoxitin); 7C782967RD (Ampicillin); K72T3FS567 (Adenosine); Q91FH1328A (Methicillin); UH95VD7V76 (Oxacillin)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170201
[St] Status:MEDLINE
[do] DOI:10.1186/s12941-017-0181-5


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[PMID]:27903603
[Au] Autor:Miller SA; Karichu J; Kohner P; Cole N; Hindler JA; Patel R; Richter S; Humphries RM
[Ad] Endereço:Department of Pathology and Laboratory Medicine, University of California-Los Angeles, Los Angeles, California, USA.
[Ti] Título:Multicenter Evaluation of a Modified Cefoxitin Disk Diffusion Method and PBP2a Testing To Predict mecA-Mediated Oxacillin Resistance in Atypical Staphylococcus aureus.
[So] Source:J Clin Microbiol;55(2):485-494, 2017 Feb.
[Is] ISSN:1098-660X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Phenotypic variants of Staphylococcus aureus that display small colonies, reduced pigmentation, and decreased hemolysis and/or coagulase activity are periodically isolated by the clinical laboratory. Antimicrobial susceptibility testing (AST) of these isolates is complicated, because many do not grow on routine AST media, including Mueller-Hinton agar (MHA) and cation-adjusted Mueller-Hinton broth. This multicenter study evaluated cefoxitin disk diffusion for 37 atypical S. aureus isolates (156 readings) with MHA supplemented with 5% sheep's blood (BMHA), using mecA PCR as the reference standard. The correlation of two commercial PBP2a assays with mecA PCR was also assessed. Ten isolates were negative and 27 positive for mecA No major errors for cefoxitin were observed, but 19.5% very major errors (VMEs) were observed at 24 h of incubation, and 17.2% VMEs were observed at 48 h. The proportions of VMEs ranged from 14.7 to 23.0% at 24 h, and from 13.3 to 17.6% at 48 h, across three testing laboratories. PBP2a tests were performed from growth on BMHA and blood agar plates (BAP), with and without cefoxitin disk induction. The Alere PBP2a SA culture colony test sensitivities for mecA were 90.0% with uninduced growth and 97.4% with induced growth from BMHA. On BAP, sensitivity was 96.0% with induced growth. The sensitivities of the Oxoid PBP2' latex agglutination test were 85.7% with uninduced growth and 93.9% with induced growth from BMHA and 95.9% with induced growth on BAP. On the basis of these data, we recommend that laboratories perform only mecA PCR and/or PBP2a tests when requested to perform AST on atypical isolates of S. aureus.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Proteínas de Bactérias/análise
Cefoxitina/farmacologia
Testes de Sensibilidade a Antimicrobianos por Disco-Difusão/métodos
Oxacilina/farmacologia
Proteínas de Ligação às Penicilinas/análise
Staphylococcus aureus/efeitos dos fármacos
Resistência beta-Lactâmica
[Mh] Termos MeSH secundário: Meios de Cultura/química
Sensibilidade e Especificidade
Staphylococcus aureus/química
Staphylococcus aureus/crescimento & desenvolvimento
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE; MULTICENTER STUDY
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Bacterial Proteins); 0 (Culture Media); 0 (Penicillin-Binding Proteins); 0 (mecA protein, Staphylococcus aureus); 6OEV9DX57Y (Cefoxitin); UH95VD7V76 (Oxacillin)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170801
[Lr] Data última revisão:
170801
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161202
[St] Status:MEDLINE
[do] DOI:10.1128/JCM.02211-16


  8 / 1805 MEDLINE  
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[PMID]:27799212
[Au] Autor:Mougari F; Bouziane F; Crockett F; Nessar R; Chau F; Veziris N; Sapriel G; Raskine L; Cambau E
[Ad] Endereço:APHP, Hôpitaux universitaires Lariboisière-Saint Louis, Service de Bactériologie, Paris, France.
[Ti] Título:Selection of Resistance to Clarithromycin in Mycobacterium abscessus Subspecies.
[So] Source:Antimicrob Agents Chemother;61(1), 2017 Jan.
[Is] ISSN:1098-6596
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Mycobacterium abscessus is an emerging pathogen against which clarithromycin is the main drug used. Clinical failures are commonly observed and were first attributed to acquired mutations in rrl encoding 23S rRNA but were then attributed to the intrinsic production of the erm(41) 23S RNA methylase. Since strains of M. abscessus were recently distributed into subspecies and erm(41) sequevars, we investigated acquired clarithromycin resistance mechanisms in mutants selected in vitro from four representative strains. Mutants were sequenced for rrl, erm(41), whiB, rpIV, and rplD and studied for seven antibiotic MICs. For mutants obtained from strain M. abscessus subsp. abscessus erm(41) T28 sequevar and strain M. abscessus subsp. bolletii, which are both known to produce effective methylase, rrl was mutated in only 19% (4/21) and 32.5% (13/40) of mutants, respectively, at position 2058 (A2058C, A2058G) or position 2059 (A2059C, A2059G). No mutations were observed in any of the other genes studied, and resistance to other antibiotics (amikacin, cefoxitin, imipenem, tigecycline, linezolid, and ciprofloxacin) was mainly unchanged. For M. abscessus subsp. abscessus erm(41) C28 sequevar and M. abscessus subsp. massiliense, not producing effective methylase, 100% (26/26) and 97.5% (39/40) of mutants had rrl mutations at position 2058 (A2058C, A2058G, A2058T) or position 2059 (A2059C, A2059G). The remaining M. abscessus subsp. massiliense mutant showed an 18-bp repeat insertion in rpIV, encoding the L22 protein. Our results showed that acquisition of clarithromycin resistance is 100% mediated by structural 50S ribosomal subunit mutations for M. abscessus subsp. abscessus erm(41) C28 and M. abscessus subsp. massiliense, whereas it is less common for M. abscessus subsp. abscessus erm(41) T28 sequevar and M. abscessus subsp. bolletii, where other mechanisms may be responsible for failure.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Claritromicina/farmacologia
Mycobacterium/efeitos dos fármacos
[Mh] Termos MeSH secundário: Amicacina/farmacologia
Cefoxitina/farmacologia
Ciprofloxacino/farmacologia
Imipenem/farmacologia
Linezolida/farmacologia
Testes de Sensibilidade Microbiana
Minociclina/análogos & derivados
Minociclina/farmacologia
Mutação/genética
Mycobacterium/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 5E8K9I0O4U (Ciprofloxacin); 6OEV9DX57Y (Cefoxitin); 70JE2N95KR (tigecycline); 71OTZ9ZE0A (Imipenem); 84319SGC3C (Amikacin); FYY3R43WGO (Minocycline); H1250JIK0A (Clarithromycin); ISQ9I6J12J (Linezolid)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170919
[Lr] Data última revisão:
170919
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161102
[St] Status:MEDLINE


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[PMID]:27716106
[Au] Autor:Aguilera Rossi CG; Gómez-Puertas P; Ayala Serrano JA
[Ad] Endereço:Departamento de Ciencias Preclínicas, Facultad de Medicina, Universidad de La Frontera, Temuco, Chile.
[Ti] Título:In vivo functional and molecular characterization of the Penicillin-Binding Protein 4 (DacB) of Pseudomonas aeruginosa.
[So] Source:BMC Microbiol;16(1):234, 2016 Oct 06.
[Is] ISSN:1471-2180
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Community and nosocomial infections by Pseudomonas aeruginosa still create a major therapeutic challenge. The resistance of this opportunist pathogen to ß-lactam antibiotics is determined mainly by production of the inactivating enzyme AmpC, a class C cephalosporinase with a regulation system more complex than those found in members of the Enterobacteriaceae family. This regulatory system also participates directly in peptidoglycan turnover and recycling. One of the regulatory mechanisms for AmpC expression, recently identified in clinical isolates, is the inactivation of LMM-PBP4 (Low-Molecular-Mass Penicillin-Binding Protein 4), a protein whose catalytic activity on natural substrates has remained uncharacterized until now. RESULTS: We carried out in vivo activity trials for LMM-PBP4 of Pseudomonas aeruginosa on macromolecular peptidoglycan of Escherichia coli and Pseudomonas aeruginosa. The results showed a decrease in the relative quantity of dimeric, trimeric and anhydrous units, and a smaller reduction in monomer disaccharide pentapeptide (M5) levels, validating the occurrence of D,D-carboxypeptidase and D,D-endopeptidase activities. Under conditions of induction for this protein and cefoxitin treatment, the reduction in M5 is not fully efficient, implying that LMM-PBP4 of Pseudomonas aeruginosa presents better behaviour as a D,D-endopeptidase. Kinetic evaluation of the direct D,D-peptidase activity of this protein on natural muropeptides M5 and D45 confirmed this bifunctionality and the greater affinity of LMM-PBP4 for its dimeric substrate. A three-dimensional model for the monomeric unit of LMM-PBP4 provided structural information which supports its catalytic performance. CONCLUSIONS: LMM-PBP4 of Pseudomonas aeruginosa is a bifunctional enzyme presenting both D,D-carboxypeptidase and D,D-endopeptidase activities; the D,D-endopeptidase function is predominant. Our study provides unprecedented functional and structural information which supports the proposal of this protein as a potential hydrolase-autolysin associated with peptidoglycan maturation and recycling. The fact that mutant PBP4 induces AmpC, may indicate that a putative muropeptide-subunit product of the DD-EPase activity of PBP4 could be a negative regulator of the pathway. This data contributes to understanding of the regulatory aspects of resistance to ß-lactam antibiotics in this bacterial model.
[Mh] Termos MeSH primário: Proteínas de Ligação às Penicilinas/fisiologia
Pseudomonas aeruginosa/metabolismo
[Mh] Termos MeSH secundário: Antibacterianos/farmacologia
Proteínas de Bactérias/genética
Proteínas de Bactérias/metabolismo
Carboxipeptidases/metabolismo
Cefoxitina/farmacologia
Infecção Hospitalar
DNA Bacteriano/genética
Endopeptidases/metabolismo
Ativação Enzimática
Escherichia coli/genética
Escherichia coli/metabolismo
Proteínas de Escherichia coli/química
Proteínas de Escherichia coli/genética
Modelos Moleculares
Proteínas de Ligação às Penicilinas/genética
Proteínas de Ligação às Penicilinas/isolamento & purificação
Proteínas de Ligação às Penicilinas/metabolismo
Peptidoglicano/metabolismo
Pseudomonas aeruginosa/enzimologia
Pseudomonas aeruginosa/genética
Proteínas Recombinantes
Resistência beta-Lactâmica/genética
beta-Lactamas/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Bacterial Proteins); 0 (DNA, Bacterial); 0 (Escherichia coli Proteins); 0 (Penicillin-Binding Proteins); 0 (Peptidoglycan); 0 (Recombinant Proteins); 0 (beta-Lactams); 6OEV9DX57Y (Cefoxitin); EC 3.4.- (Carboxypeptidases); EC 3.4.- (Endopeptidases)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170626
[Lr] Data última revisão:
170626
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161008
[St] Status:MEDLINE


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[PMID]:27650515
[Au] Autor:Ho CM; Lin CY; Ho MW; Lin HC; Chen CJ; Lin LC; Lu JJ
[Ad] Endereço:Department of Laboratory Medicine, China Medical University Hospital, Taichung, Taiwan; Department of Internal Medicine, China Medical University Hospital, Taichung, Taiwan; Department of Nursing, Hungkuang University, Taichung, Taiwan; Graduate Institute of Clinical Medical Science, China Medical U
[Ti] Título:Methicillin-resistant Staphylococcus aureus isolates with SCCmec type V and spa types t437 or t1081 associated to discordant susceptibility results between oxacillin and cefoxitin, Central Taiwan.
[So] Source:Diagn Microbiol Infect Dis;86(4):405-411, 2016 Dec.
[Is] ISSN:1879-0070
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Staphylococcus aureus isolates with discordant susceptibility results between oxacillin and cefoxitin obtained using automated microbiology systems are infrequently observed. From April 2013 to December 2014, 1956 methicillin-resistant S. aureus (MRSA) and 1761 methicillin-susceptible S. aureus isolates were obtained from different patients. Forty isolates (1.1% and 2% in case of S. aureus and MRSA, respectively) with discordant susceptibility results (oxacillin susceptible and cefoxitin resistant) and carrying mecA gene were obtained. Except 2 SCCmec type IV isolates, 38 MRSA isolates were all SCCmec type V (V or non-V ), which were further divided into V (n=22) and non-V (n=16). The most common spa type in V and non-V isolates were t437 (n=19) and t1081 (n=13), respectively. Only 55% of patients received effective antimicrobial agents; 2 mortalities were not attributable to MRSA infection. Using standard agar dilution, 17 MRSA isolates (0.46% and 0.87% in case of S. aureus and MRSA, respectively) had oxacillin MIC in the susceptible ranges (oxacillin-susceptible MRSA [OS-MRSA]); all carried SCCmec type V (V , n=8; non-V , n=9). The most common spa-MLST types of OS-MRSA in V and non-V were t437-ST59 (n=4) and t1081-ST45 (n=7), respectively. Concomitant testing by both cefoxitin- and oxacillin-based methods is a practical strategy for OS-MRSA detection in the clinical laboratories. Continuous monitoring of OS-MRSA isolates is necessary to elucidate their impact in clinical infectious diseases.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Cefoxitina/farmacologia
Genótipo
Staphylococcus aureus Resistente à Meticilina/classificação
Staphylococcus aureus Resistente à Meticilina/efeitos dos fármacos
Oxacilina/farmacologia
Infecções Estafilocócicas/microbiologia
[Mh] Termos MeSH secundário: Adolescente
Adulto
Idoso
Idoso de 80 Anos ou mais
Criança
Pré-Escolar
Feminino
Seres Humanos
Lactente
Recém-Nascido
Masculino
Staphylococcus aureus Resistente à Meticilina/genética
Staphylococcus aureus Resistente à Meticilina/isolamento & purificação
Testes de Sensibilidade Microbiana
Meia-Idade
Taiwan
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 6OEV9DX57Y (Cefoxitin); UH95VD7V76 (Oxacillin)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170208
[Lr] Data última revisão:
170208
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160922
[St] Status:MEDLINE



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