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[PMID]:25815670
[Au] Autor:Nasr T; Bondock S; Eid S
[Ad] Endereço:a Department of Pharmaceutical Chemistry, Faculty of Pharmacy , Helwan University , Helwan , Cairo , Egypt .
[Ti] Título:Design, synthesis, antimicrobial evaluation and molecular docking studies of some new 2,3-dihydrothiazoles and 4-thiazolidinones containing sulfisoxazole.
[So] Source:J Enzyme Inhib Med Chem;31(2):236-46, 2016.
[Is] ISSN:1475-6374
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Microbial resistance to the available drugs poses a serious threat in modern medicine. We report the design, synthesis and in vitro antimicrobial evaluation of new functionalized 2,3-dihydrothiazoles and 4-thiazolidinones tagged with sulfisoxazole moiety. Compound 8d was most active against Bacillis subtilis (MIC, 0.007 µg/mL). Moreover, compounds 7c-d and 8c displayed significant activities against B. subtilis and Streptococcus pneumoniae (MIC, 0.03-0.06 µg/mL and 0.06-0.12 µg/mL versus ampicillin 0.24 µg/mL and 0.12 µg/mL; respectively). Compounds 7a and 7c-d were highly potent against Escherichia coli (MIC, 0.49-0.98 µg/mL versus gentamycin 1.95 µg/mL). On the other hand, compounds 7e and 9c were fourfolds more active than amphotericin B against Syncephalastrum racemosum. Molecular docking studies showed that the synthesized compounds could act as inhibitors for the dihydropteroate synthase enzyme (DHPS). This study is a platform for the future design of more potent antimicrobial agents.
[Mh] Termos MeSH primário: Anti-Infecciosos/química
Anti-Infecciosos/farmacologia
Relação Estrutura-Atividade
Tiazóis/química
[Mh] Termos MeSH secundário: Anti-Infecciosos/síntese química
Sítios de Ligação
Técnicas de Química Sintética
Di-Hidropteroato Sintase/antagonistas & inibidores
Di-Hidropteroato Sintase/química
Di-Hidropteroato Sintase/metabolismo
Desenho de Drogas
Avaliação Pré-Clínica de Medicamentos/métodos
Inibidores Enzimáticos/química
Inibidores Enzimáticos/farmacologia
Testes de Sensibilidade Microbiana
Simulação de Acoplamento Molecular
Sulfisoxazol/química
Tiazóis/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Infective Agents); 0 (Enzyme Inhibitors); 0 (Thiazoles); 6569-17-1 (2,3-dihydrothiazole); 740T4C525W (Sulfisoxazole); EC 2.5.1.15 (Dihydropteroate Synthase)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150328
[St] Status:MEDLINE
[do] DOI:10.3109/14756366.2015.1016514


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[PMID]:28189110
[Au] Autor:Karimi-Maleh H; Amini F; Akbari A; Shojaei M
[Ad] Endereço:Department of Chemistry, Graduate University of Advanced Technology, Kerman, Iran. Electronic address: h.karimi.maleh@gmail.com.
[Ti] Título:Amplified electrochemical sensor employing CuO/SWCNTs and 1-butyl-3-methylimidazolium hexafluorophosphate for selective analysis of sulfisoxazole in the presence of folic acid.
[So] Source:J Colloid Interface Sci;495:61-67, 2017 06 01.
[Is] ISSN:1095-7103
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In the present work, CuO nanoparticle decorated on single wall carbon nanotubes (CuO/SWCNTs) nanocomposite was successfully synthesized by chemical precipitation method and used for modification of carbon paste electrode (CPE) in the presence of 1-butyl-3-methylimidazolium hexafluorophosphate (1-B-3-MIHFP) liquid as binder. The novel voltammetric sensor was used as first electrochemical sensor for determination of sulfisoxazole (SFX). CuO/SWCNTs nanocomposite characterized by scanning electron microscopy (SEM) and X-ray powder diffraction (XRD) methods. Voltammetric methods such as cyclic voltammetry, square wave voltammetry (SWV), electrochemical impedance spectroscopy (EIS) and chronoamperometry were performed to assess the electrochemical performance of CuO/SWCNTs/1-B-3-MIHFP/CPE towards SFX in aqueous solution. The voltammetric obtained data confirm the significant enhancement of oxidation current and reduction overvoltage for electro-oxidation of SFX at a surface of CuO/SWCNTs/1-B-3-MIHFP/CPE. The square wave voltammetric response shows the linear increment of oxidation signals with an increase in the concentration of SFX in the range of 0.08-650µM with limit of detection 0.04µM. Using CuO/SWCNTs/1-B-3-MIHFP/CPE the SFX and folic acid peaks are separated ca. 0.72 and 0.895V, respectively; hence SFX can be detected in the presence of folic acid. Finally, the CuO/SWCNTs/1-B-3-MIHFP/CPE was used as high sensitive tools for analysis of SFX and folic acid in real samples.
[Mh] Termos MeSH primário: Antibacterianos/análise
Técnicas Biossensoriais
Cobre/química
Ácido Fólico/análise
Imidazóis/química
Nanocompostos/química
Nanotubos de Carbono/química
Sulfisoxazol/análise
[Mh] Termos MeSH secundário: Espectroscopia Dielétrica
Técnicas Eletroquímicas
Eletrodos
Limite de Detecção
Oxirredução
Difração de Raios X
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (1-butyl-3-methylimidazolium hexafluorophosphate); 0 (Anti-Bacterial Agents); 0 (Imidazoles); 0 (Nanotubes, Carbon); 740T4C525W (Sulfisoxazole); 789U1901C5 (Copper); 935E97BOY8 (Folic Acid); V1XJQ704R4 (cupric oxide)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170921
[Lr] Data última revisão:
170921
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170212
[St] Status:MEDLINE


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[PMID]:27746217
[Au] Autor:Amini N; Vousooghi N; Soleimani M; Samadikuchaksaraei A; Akbari M; Safakheil H; Atafimanesh P; Shahbazi A; Brouki Milan P; Ramezani S; Mozafari M; Joghataei MT
[Ad] Endereço:Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran; Department of Neuroscience, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
[Ti] Título:A new rat model of neonatal bilirubin encephalopathy (kernicterus).
[So] Source:J Pharmacol Toxicol Methods;84:44-50, 2017 Mar - Apr.
[Is] ISSN:1873-488X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:INTRODUCTION: Hemolytic kernicterus, an indirect bilirubin-induced brain dysfunction, is associated with hyper-bilirubinemia in mammalian neonates. In this study, a new model of kernicterus has been developed using intra-peritoneal injections of phenyl hydrazine and subcutaneous injections of sulfisoxazole. These drugs can potentially induce kernicterus in neonatal through changes in hemolysis and hypo-albumin. METHODS: For this purpose, 7-day-old male Wistar rats (n=72; mean weight 11±1g) were used. The animals have been divided into six different groups which received the drugs alone and their combination, and the drugs' solvents and their combination. Biochemical parameters, brain iron and bilirubin, behavioural performance, auditory function and apoptosis were measured using auto-analyser instruments; atomic absorption spectroscopy, Sawasaki, footprint, auditory brainstem response (ABR) and TUNEL test, respectively. RESULT: The drug-injected groups showed a significant reduction in serum haematocrit and an increase in the concentration of brain bilirubin, total and indirect bilirubin as well as TUNEL positive cells in basal ganglia. In addition, the obtained results showed that there was a significant increase in behavioural disturbance and auditory dysfunction in the group injected with the combination of two drugs. CONCLUSION: This kernicterus-induced rat model could perfectly mimic the common conditions of the hyperbilirubinemia in human neonates. This study offers an easy technique to develop more stable models for follow-up studies.
[Mh] Termos MeSH primário: Bilirrubina/metabolismo
Modelos Animais de Doenças
Kernicterus/induzido quimicamente
Kernicterus/metabolismo
[Mh] Termos MeSH secundário: Animais
Animais Recém-Nascidos
Potenciais Evocados Auditivos do Tronco Encefálico/efeitos dos fármacos
Potenciais Evocados Auditivos do Tronco Encefálico/fisiologia
Kernicterus/patologia
Masculino
Fenil-Hidrazinas/toxicidade
Distribuição Aleatória
Ratos
Ratos Wistar
Sulfisoxazol/toxicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Phenylhydrazines); 064F424C9K (phenylhydrazine); 740T4C525W (Sulfisoxazole); RFM9X3LJ49 (Bilirubin)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170707
[Lr] Data última revisão:
170707
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161018
[St] Status:MEDLINE


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[PMID]:27755598
[Au] Autor:Pornsukarom S; Thakur S
[Ad] Endereço:Department of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina, 27607, United States of America.
[Ti] Título:Assessing the Impact of Manure Application in Commercial Swine Farms on the Transmission of Antimicrobial Resistant Salmonella in the Environment.
[So] Source:PLoS One;11(10):e0164621, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Land application of swine manure in commercial hog farms is an integral part of their waste management system which recycles the nutrients back to the soil. However, manure application can lead to the dissemination of bacterial pathogens in the environment and pose a serious public health threat. The aim of this study was to determine the dissemination of antimicrobial resistant Salmonella in the environment due to manure application in commercial swine farms in North Carolina (n = 6) and Iowa (n = 7), two leading pork producing states in the US. We collected manure and soil samples twice on day 0 (before and after manure application) from four distinct plots of lands (5 soil samples/plot) located at 20 feet away from each other in the field. Subsequent soil samples were collected again on days 7, 14, 21 from the same plots. A total of 1,300 soil samples (NC = 600; IA = 700) and 130 manure samples (NC = 60; IA = 70) were collected and analyzed in this study. The overall Salmonella prevalence was 13.22% (189/1,430), represented by 10.69% and 38.46% prevalence in soil and manure, respectively. The prevalence in NC (25.45%) was significantly higher than in IA (2.73%) (P<0.001) and a consistent decrease in Salmonella prevalence was detected from Day 0-Day 21 in all the farms that tested positive. Salmonella serotypes detected in NC were not detected in IA, thereby highlighting serotype association based on manure storage and soil application method used in the two regions. Antimicrobial susceptibility testing was done by the broth microdilution method to a panel of 15 antimicrobial drugs. A high frequency of isolates (58.73%) were multidrug resistant (resistance to three or more class of antimicrobials) and the most frequent resistance was detected against streptomycin (88.36%), sulfisoxazole (67.2%), and tetracycline (57.67%). Genotypic characterization by pulse field gel electrophoresis revealed clonally related Salmonella in both manure and soil at multiple time points in the positive farms. Our study highlights the potential role of swine manure application in the dissemination and persistence of antimicrobial resistant Salmonella in the environment.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Farmacorresistência Bacteriana Múltipla/efeitos dos fármacos
Esterco/microbiologia
Salmonelose Animal/transmissão
Salmonella/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Eletroforese em Gel de Campo Pulsado
Meio Ambiente
Testes de Sensibilidade Microbiana
Filogenia
Salmonella/classificação
Salmonella/isolamento & purificação
Salmonelose Animal/microbiologia
Salmonelose Animal/patologia
Sorogrupo
Estreptomicina/farmacologia
Sulfisoxazol/farmacologia
Suínos
Doenças dos Suínos/microbiologia
Doenças dos Suínos/patologia
Tetraciclina/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Manure); 740T4C525W (Sulfisoxazole); F8VB5M810T (Tetracycline); Y45QSO73OB (Streptomycin)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170531
[Lr] Data última revisão:
170531
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161019
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0164621


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[PMID]:27454084
[Au] Autor:Oh K; Baek MC; Kang W
[Ad] Endereço:College of Pharmacy, Chung-Ang University, Seoul, South Korea.
[Ti] Título:Quantitative determination of sulfisoxazole and its three N-acetylated metabolites using HPLC-MS/MS, and the saturable pharmacokinetics of sulfisoxazole in mice.
[So] Source:J Pharm Biomed Anal;129:332-338, 2016 Sep 10.
[Is] ISSN:1873-264X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Sulfisoxazole (SFX) is still used in combination with trimethoprim in cattle despite adverse drug reactions (e.g., urolithiasis). Recently, SFX is known to be a promising repositioned drug candidate for pulmonary hypertension and cancer. We developed a simultaneous determination method of SFX and its N-acetylated metabolites (N(1)-acetyl SFX, N1AS; N(4)-acetyl SFX, N4AS; diacetyl SFX, DAS) using HPLC-MS/MS for the first time, and examined the pharmacokinetics of SFX in mice. N1AS and DAS were converted rapidly to SFX and N4AS, respectively, in mouse plasma. The time courses of plasma SFX and N4AS concentrations were well-characterised following the oral administration of SFX to mice. The absorption, metabolism, and/or excretion of SFX given at >700mg/kg may be saturable, and in contrast to humans and rats, the extent of systemic exposure of mice to N4AS was much greater than that of SFX. Interestingly, the acetyl groups at both N1- and N4-positions were degraded during the ionisation required to generate precursor ions. In additional experiments the carboxyl group of N-acetyl-5-aminosalicylic acid (NA5AS) was lost instead of the acetyl group during the ionisation, and acetaminophen (AAP) appeared. As the acetyl and carboxyl groups of some substances can be degraded during ionisation in the mass spectrometer, caution is appropriate when it is sought to simultaneously quantify similar structures containing these moieties; chromatographic separation is essential.
[Mh] Termos MeSH primário: Sulfisoxazol/química
Sulfisoxazol/farmacocinética
[Mh] Termos MeSH secundário: Acetaminofen/química
Acetaminofen/farmacocinética
Administração Oral
Ácido Aminossalicílico/química
Ácido Aminossalicílico/farmacocinética
Animais
Cromatografia Líquida de Alta Pressão/métodos
Seres Humanos
Camundongos
Ratos
Espectrometria de Massas em Tandem/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
362O9ITL9D (Acetaminophen); 5B2658E0N2 (Aminosalicylic Acid); 740T4C525W (Sulfisoxazole)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170808
[Lr] Data última revisão:
170808
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160726
[St] Status:MEDLINE


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[PMID]:27423008
[Au] Autor:Kim E; Kang W
[Ad] Endereço:College of Pharmacy, Chung-Ang University, Seoul 06974, South Korea.
[Ti] Título:Simultaneous determination of N(1)-acetyl sulfisoxazole and its metabolites, and relative bioavailability compare to sulfisoxazole in rats.
[So] Source:J Pharm Biomed Anal;129:117-120, 2016 Sep 10.
[Is] ISSN:1873-264X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:N(1)-acetyl sulfisoxazole (N1AS), a dihydropteroate synthase inhibitor is known to be biotransformed primarily to sulfisoxazole, partly to N(4)-acetyl sulfisoxazole (N4AS), and likely also to diacetyl sulfisoxazole (DAS) and other compounds. Although its clinical use has been limited due to urolithiasis, some countries still use the drug in combination with trimethoprim in cattle. A liquid chromatographic method using ultraviolet detection was developed for the simultaneous determination of four substances for the first time. Four analytes and sulfamethoxazole (IS) were separated on a reversed-phase column with gradient elution of a mobile phase. Because DAS and N1AS in plasma were changed very quickly into N4AS and sulfisoxazole, respectively, and esterase inhibitors (sodium fluoride and eserine) could not prevent the transformation, sulfisoxazole and N4AS were monitored in rat plasma following a single oral administration of N1AS and sulfisoxazole in five rats. The relative bioavailability of N1AS to sulfisoxazole was about two, indicating that a half-dose of N1AS would be equivalent to a dose of sulfisoxazole to achieve the same systemic exposure to sulfisoxazole.
[Mh] Termos MeSH primário: Sulfisoxazol/análogos & derivados
Sulfisoxazol/análise
Sulfisoxazol/metabolismo
[Mh] Termos MeSH secundário: Administração Oral
Animais
Disponibilidade Biológica
Cromatografia Líquida de Alta Pressão/métodos
Ratos
Ratos Sprague-Dawley
Sulfisoxazol/administração & dosagem
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
740T4C525W (Sulfisoxazole); WBT5QH3KED (acetyl sulfisoxazole)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170808
[Lr] Data última revisão:
170808
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160717
[St] Status:MEDLINE


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[PMID]:27155409
[Au] Autor:Wang Y; Liu S; Li R; Huang Y; Chen C
[Ad] Endereço:Collaborative Innovation Center for Geo-Hazards and Eco-Environment in Three Gorges Area, Yichang 443002, China.
[Ti] Título:Electro-catalytic degradation of sulfisoxazole by using graphene anode.
[So] Source:J Environ Sci (China);43:54-60, 2016 May.
[Is] ISSN:1001-0742
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Graphite and graphene electrodes were prepared by using pure graphite as precursor. The electrode materials were characterized by a scanning electron microscope (SEM), X-ray diffraction (XRD) and cyclic voltammetry (CV) measurements. The electro-catalytic activity for degradation of sulfisoxazole (SIZ) was investigated by using prepared graphene or graphite anode. The results showed that the degradation of SIZ was much more rapid on the graphene than that on the graphite electrode. Moreover, the graphene electrode exhibited good stability and recyclability. The analysis on the intermediate products and the measurement of active species during the SIZ degradation demonstrated that indirect oxidation is the dominant mechanism, involving the electro-catalytic generation of OH and O2(-) as the main active oxygen species. This study implies that graphene is a promising potential electrode material for long-term application to electro-catalytic degradation of organic pollutants.
[Mh] Termos MeSH primário: Eletrodos
Poluentes Ambientais/química
Grafite/química
Sulfisoxazol/química
[Mh] Termos MeSH secundário: Técnicas Eletroquímicas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Environmental Pollutants); 740T4C525W (Sulfisoxazole); 7782-42-5 (Graphite)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170805
[Lr] Data última revisão:
170805
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160508
[St] Status:MEDLINE


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[PMID]:26818600
[Au] Autor:Amini N; Vousooghi N; Hadjighassem M; Bakhtiyari M; Mousavi N; Safakheil H; Jafari L; Sarveazad A; Yari A; Ramezani S; Faghihi F; Joghataei MT
[Ad] Endereço:Department of Neuroscience, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
[Ti] Título:Efficacy of Human Adipose Tissue-Derived Stem Cells on Neonatal Bilirubin Encephalopathy in Rats.
[So] Source:Neurotox Res;29(4):514-24, 2016 May.
[Is] ISSN:1476-3524
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Kernicterus is a neurological syndrome associated with indirect bilirubin accumulation and damages to the basal ganglia, cerebellum and brain stem nuclei particularly the cochlear nucleus. To mimic haemolysis in a rat model such that it was similar to what is observed in a preterm human, we injected phenylhydrazine in 7-day-old rats to induce haemolysis and then infused sulfisoxazole into the same rats at day 9 to block bilirubin binding sites in the albumin. We have investigated the effectiveness of human adiposity-derived stem cells as a therapeutic paradigm for perinatal neuronal repair in a kernicterus animal model. The level of total bilirubin, indirect bilirubin, brain bilirubin and brain iron was significantly increased in the modelling group. There was a significant decreased in all severity levels of the auditory brainstem response test in the two modelling group. Akinesia, bradykinesia and slip were significantly declined in the experience group. Apoptosis in basal ganglia and cerebellum were significantly decreased in the stem cell-treated group in comparison to the vehicle group. All severity levels of the auditory brainstem response tests were significantly decreased in 2-month-old rats. Transplantation results in the substantial alleviation of walking impairment, apoptosis and auditory dysfunction. This study provides important information for the development of therapeutic strategies using human adiposity-derived stem cells in prenatal brain damage to reduce potential sensori motor deficit.
[Mh] Termos MeSH primário: Tecido Adiposo/citologia
Kernicterus/cirurgia
Transplante de Células-Tronco/métodos
Células-Tronco/fisiologia
[Mh] Termos MeSH secundário: Animais
Animais Recém-Nascidos
Anti-Infecciosos/toxicidade
Antígenos CD/metabolismo
Encéfalo/citologia
Encéfalo/metabolismo
Modelos Animais de Doenças
Citometria de Fluxo
Seres Humanos
Ferro/metabolismo
Kernicterus/induzido quimicamente
Kernicterus/complicações
Masculino
Oxidantes/toxicidade
Fenil-Hidrazinas/toxicidade
Ratos
Ratos Wistar
Filtro Sensorial/efeitos dos fármacos
Sulfisoxazol/toxicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Infective Agents); 0 (Antigens, CD); 0 (Oxidants); 0 (Phenylhydrazines); 064F424C9K (phenylhydrazine); 740T4C525W (Sulfisoxazole); E1UOL152H7 (Iron)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:171110
[Lr] Data última revisão:
171110
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160129
[St] Status:MEDLINE
[do] DOI:10.1007/s12640-016-9599-3


  9 / 710 MEDLINE  
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[PMID]:25957382
[Au] Autor:Bossé JT; Li Y; Walker S; Atherton T; Fernandez Crespo R; Williamson SM; Rogers J; Chaudhuri RR; Weinert LA; Oshota O; Holden MT; Maskell DJ; Tucker AW; Wren BW; Rycroft AN; Langford PR; BRaDP1T Consortium
[Ad] Endereço:Section of Paediatrics, Department of Medicine, Imperial College London, St Mary's Campus, London W2 1PG, UK j.bosse@imperial.ac.uk.
[Ti] Título:Identification of dfrA14 in two distinct plasmids conferring trimethoprim resistance in Actinobacillus pleuropneumoniae.
[So] Source:J Antimicrob Chemother;70(8):2217-22, 2015 Aug.
[Is] ISSN:1460-2091
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:OBJECTIVES: The objective of this study was to determine the distribution and genetic basis of trimethoprim resistance in Actinobacillus pleuropneumoniae isolates from pigs in England. METHODS: Clinical isolates collected between 1998 and 2011 were tested for resistance to trimethoprim and sulphonamide. The genetic basis of trimethoprim resistance was determined by shotgun WGS analysis and the subsequent isolation and sequencing of plasmids. RESULTS: A total of 16 (out of 106) A. pleuropneumoniae isolates were resistant to both trimethoprim (MIC >32 mg/L) and sulfisoxazole (MIC ≥256 mg/L), and a further 32 were resistant only to sulfisoxazole (MIC ≥256 mg/L). Genome sequence data for the trimethoprim-resistant isolates revealed the presence of the dfrA14 dihydrofolate reductase gene. The distribution of plasmid sequences in multiple contigs suggested the presence of two distinct dfrA14-containing plasmids in different isolates, which was confirmed by plasmid isolation and sequencing. Both plasmids encoded mobilization genes, the sulphonamide resistance gene sul2, as well as dfrA14 inserted into strA, a streptomycin-resistance-associated gene, although the gene order differed between the two plasmids. One of the plasmids further encoded the strB streptomycin-resistance-associated gene. CONCLUSIONS: This is the first description of mobilizable plasmids conferring trimethoprim resistance in A. pleuropneumoniae and, to our knowledge, the first report of dfrA14 in any member of the Pasteurellaceae. The identification of dfrA14 conferring trimethoprim resistance in A. pleuropneumoniae isolates will facilitate PCR screens for resistance to this important antimicrobial.
[Mh] Termos MeSH primário: Infecções por Actinobacillus/veterinária
Actinobacillus pleuropneumoniae/efeitos dos fármacos
Plasmídeos
Doenças dos Suínos/microbiologia
Tetra-Hidrofolato Desidrogenase/genética
Resistência a Trimetoprima
[Mh] Termos MeSH secundário: Infecções por Actinobacillus/microbiologia
Actinobacillus pleuropneumoniae/enzimologia
Actinobacillus pleuropneumoniae/genética
Actinobacillus pleuropneumoniae/isolamento & purificação
Animais
Anti-Infecciosos/farmacologia
Inglaterra
Genoma Bacteriano
Testes de Sensibilidade Microbiana
Dados de Sequência Molecular
Análise de Sequência de DNA
Sulfisoxazol/farmacologia
Suínos
Trimetoprima/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Infective Agents); 740T4C525W (Sulfisoxazole); AN164J8Y0X (Trimethoprim); EC 1.5.1.3 (Tetrahydrofolate Dehydrogenase)
[Em] Mês de entrada:1604
[Cu] Atualização por classe:161019
[Lr] Data última revisão:
161019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150510
[St] Status:MEDLINE
[do] DOI:10.1093/jac/dkv121


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[PMID]:25893618
[Au] Autor:Maszkowska J; Bialk-Bielinska A; Mioduszewska K; Wagil M; Kumirska J; Stepnowski P
[Ad] Endereço:Department of Environmental Analysis, Faculty of Chemistry, University of Gdansk, ul. Wita Stwosza 63, 80-308, Gdansk, Poland.
[Ti] Título:Sorption of sulfisoxazole onto soil--an insight into different influencing factors.
[So] Source:Environ Sci Pollut Res Int;22(16):12182-9, 2015 Aug.
[Is] ISSN:1614-7499
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Although sulfonamides (SAs) are among the most commonly used veterinary drugs and their presence in the environment is well documented, knowledge of their fate and behavior in the soil environment is still limited, especially for sulfisoxazole (SSX) which is characterized by the lowest (among other SAs) pK a value associated with acid-base equilibrium of sulfonamide group. Thus, this work was focused on determining the sorption potential of SSX onto natural soils differing in physicochemical properties. All the results were modeled using linear, Freundlich, Langmuir, Dubinin-Radushkevich, and Temkin sorption isotherms. The established sorption coefficients (K(d)) for SSX were quite low (from 0.27 to 0.95 L kg(-1)), which indicated that this substance is highly mobile and has the potential to run off into surface waters and/or infiltrate ground water. The sorption data of SSX is well fitted to the Freundlich isotherm model (R(2) > 0.968). Moreover, we assessed the sorption mechanism of these compounds in the edaphic environment with respect to organic matter (OM) content, pH, and ionic strength. To clarify the current state of knowledge, these factors were examined much more thoroughly than in previous investigations concerning other SAs. The wide range of ionic strength examined showed positive correlation of this factor and sorption of SAs. The results also yielded new insight into dependency of sorption of SAs on organic matter content in soil.
[Mh] Termos MeSH primário: Poluentes do Solo/química
Solo/química
Sulfisoxazol/química
Drogas Veterinárias/química
[Mh] Termos MeSH secundário: Equilíbrio Ácido-Base
Adsorção
Modelos Químicos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Soil); 0 (Soil Pollutants); 0 (Veterinary Drugs); 740T4C525W (Sulfisoxazole)
[Em] Mês de entrada:1603
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150421
[St] Status:MEDLINE
[do] DOI:10.1007/s11356-015-4445-3



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