Base de dados : MEDLINE
Pesquisa : D02.078.100.260 [Categoria DeCS]
Referências encontradas : 608 [refinar]
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[PMID]:28470520
[Au] Autor:Suganuma K; Molefe NI; Inoue N
[Ad] Endereço:National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada, Obihiro, Hokkaido, 080-8555, Japan. k.suganuma@obihiro.ac.jp.
[Ti] Título:An ATP-Based Luciferase Viability Assay for Animal African Trypanosomes Using a 96-Well Plate.
[So] Source:Methods Mol Biol;1601:89-95, 2017.
[Is] ISSN:1940-6029
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Cell viability assays using multi-well cell culture plates are frequently used for in vitro drug screening. We herein describe an ATP-based luciferase viability assay for animal African trypanosomes using a 96-well plate. This assay could be further applied to the screening of novel compounds for the treatment of animal African trypanosomiasis.
[Mh] Termos MeSH primário: Sobrevivência Celular/efeitos dos fármacos
Tripanossomicidas/farmacologia
Trypanosoma brucei brucei/efeitos dos fármacos
Trypanosoma congolense/efeitos dos fármacos
Tripanossomíase Africana/veterinária
[Mh] Termos MeSH secundário: Trifosfato de Adenosina/análise
Trifosfato de Adenosina/metabolismo
Animais
Diminazena/análogos & derivados
Diminazena/farmacologia
Avaliação Pré-Clínica de Medicamentos/instrumentação
Avaliação Pré-Clínica de Medicamentos/métodos
Seres Humanos
Concentração Inibidora 50
Luciferases/metabolismo
Pentamidina/farmacologia
Tripanossomíase Africana/tratamento farmacológico
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Trypanocidal Agents); 673LC5J4LQ (Pentamidine); 8L70Q75FXE (Adenosine Triphosphate); EC 1.13.12.- (Luciferases); JI8SAD85NO (diminazene aceturate); Y5G36EEA5Z (Diminazene)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180219
[Lr] Data última revisão:
180219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1007/978-1-4939-6960-9_8


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[PMID]:29309784
[Au] Autor:Bassi PB; de Araújo FF; Garcia GC; Vinícius da Silva M; Oliveira CJF; Bittar ER; de Souza Gomes M; Rodrigues do Amaral L; Costa E Silva MF; Nascentes GAN; Rodrigues Junior V; Martins-Filho OA; Araújo MSS; Bittar JFF
[Ad] Endereço:Universidade de Uberaba (UNIUBE), Medicina Veterinária, Mestrado em Sanidade e Produção Animal nos Trópicos - Avenida Nenê Sabino 1697/1698, 38055-500, Uberaba, MG, Brazil. Electronic address: vet.bassi@gmail.com.
[Ti] Título:Parasitological and immunological evaluation of cattle experimentally infected with Trypanosoma vivax.
[So] Source:Exp Parasitol;185:98-106, 2018 Feb.
[Is] ISSN:1090-2449
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Trypanosoma vivax infection causes relevant economical impact due to high morbidity and mortality leading to negative impact on local livestock. Despite parasitological and serological methods are used for the diagnosis of T. vivax infection, gaps regarding sensitivity and specificity of these methods still represent a challenge. The present study aimed to compare the kinetics of parasitological and serological parameters in cattle experimentally infected with T. vivax along with immunophenotypic analysis of whole blood leukocytes. Based on the parasitemia profile the analysis were performed in three distinct periods, referred as pre-patent, patent and post-treatment. Distinct kinetics of anti-T. vivax IgM and IgG were observed during the pre-patent, patent and post-treatment periods. Increased levels of WC1 γδ T-cells were observed throughout the infection with strong correlations with other biomarkers observed during post-treatment period. Our findings demonstrated that there is a important participation of Monocytes:CD14 ; NK-cells:CD335 and WC1 γδ T-cells that coincide with the peak of parasitemia and also with the adaptive immunity, specially CD4 T-cells in T. vivax infection. The knowledge of the immune response is important not only for understanding the biology of the parasite in the host, but for the design of new treatment strategies for trypanosome infections.
[Mh] Termos MeSH primário: Doenças dos Bovinos/imunologia
Parasitemia/veterinária
Trypanosoma vivax/imunologia
Tripanossomíase Africana/veterinária
[Mh] Termos MeSH secundário: Imunidade Adaptativa
Animais
Anticorpos Antiprotozoários/sangue
Biomarcadores/análise
Bovinos
Doenças dos Bovinos/tratamento farmacológico
Doenças dos Bovinos/parasitologia
Diminazena/uso terapêutico
Técnica Indireta de Fluorescência para Anticorpo/veterinária
Imunidade Inata
Imunoglobulina G/sangue
Imunoglobulina M/sangue
Imunofenotipagem/veterinária
Leucócitos/classificação
Leucócitos/imunologia
Masculino
Parasitemia/tratamento farmacológico
Parasitemia/imunologia
Parasitemia/parasitologia
Distribuição Aleatória
Tripanossomicidas/uso terapêutico
Tripanossomíase Africana/tratamento farmacológico
Tripanossomíase Africana/imunologia
Tripanossomíase Africana/parasitologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Protozoan); 0 (Biomarkers); 0 (Immunoglobulin G); 0 (Immunoglobulin M); 0 (Trypanocidal Agents); Y5G36EEA5Z (Diminazene)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180214
[Lr] Data última revisão:
180214
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180109
[St] Status:MEDLINE


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[PMID]:28655583
[Au] Autor:Rizk MA; El-Sayed SAE; AbouLaila M; Yokoyama N; Igarashi I
[Ad] Endereço:National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-Cho, Obihiro, Hokkaido, Japan; Department of Internal Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt.
[Ti] Título:Evaluation of the inhibitory effect of N-acetyl-L-cysteine on Babesia and Theileria parasites.
[So] Source:Exp Parasitol;179:43-48, 2017 Aug.
[Is] ISSN:1090-2449
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:N-acetyl-L-cysteine is known to have antibacterial, antiviral, antimalarial, and antioxidant activities. Therefore, the in vitro inhibitory effect of this hit was evaluated in the present study on the growth of Babesia and Theileria parasites. The in vitro growth of Babesia bovis, Babesia bigemina, Babesia divergens, Theileria equi, and Babesia caballi that were tested was significantly inhibited (P < 0.05) by micromolar concentrations of N-acetyl-L-cysteine. The inhibitory effect of N-acetyl-L-cysteine was synergistically potentiated when used in combination with diminazene aceturate on B. bovis and B. caballi cultures. These results indicate that N-acetyl-L-cysteine might be used as a drug for the treatment of babesiosis, especially when used in combination with diminazene aceturate.
[Mh] Termos MeSH primário: Acetilcisteína/farmacologia
Antiprotozoários/farmacologia
Babesia/efeitos dos fármacos
Diminazena/análogos & derivados
Theileria/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Babesia/crescimento & desenvolvimento
Babesia bovis/efeitos dos fármacos
Babesia bovis/crescimento & desenvolvimento
Bovinos
Diminazena/farmacologia
Sinergismo Farmacológico
Eritrócitos/parasitologia
Cavalos
Concentração Inibidora 50
Espectrometria de Fluorescência
Theileria/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antiprotozoal Agents); JI8SAD85NO (diminazene aceturate); WYQ7N0BPYC (Acetylcysteine); Y5G36EEA5Z (Diminazene)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170726
[Lr] Data última revisão:
170726
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170629
[St] Status:MEDLINE


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[PMID]:28364836
[Au] Autor:Jaimes-Dueñez J; Triana-Chávez O; Valencia-Hernández A; Sánchez-Arévalo D; Poche-Ceballos A; Ortíz-Álvarez J; Mejía-Jaramillo AM
[Ad] Endereço:Grupo BCEI, Universidad de Antioquia UdeA, Calle 70 No. 52-21, Medellín, Colombia. Electronic address: jeiczon05@gmail.com.
[Ti] Título:Molecular diagnosis and phylogeographic analysis of Trypanosoma evansi in dogs (Canis lupus familiaris) suggest an epidemiological importance of this species in Colombia.
[So] Source:Prev Vet Med;139(Pt A):82-89, 2017 Apr 01.
[Is] ISSN:1873-1716
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Surra disease is a zoonosis caused by Trypanosoma (Trypanozoon) evansi, a salivary trypanosome, originally from Africa, which affects a wide range of mammalian worldwide. Dogs are highly susceptible to T. evansi infection and they often exhibit strong clinical signs than can lead to death, even within weeks in untreated acute cases. The present survey is the first report through clinical, parasitological and molecular approaches, of two fatal cases of T. evansi in Colombian dogs. After analysing two presumptive cases of infection with Trypanosoma spp., in dogs by parasitological methods, we confirmed by molecular techniques the presence of T. evansi, finding clinical signs such as anaemia, thrombocytopenia and hepatosplenomegaly, with fatal outcomes within a week even after the treatment. A phylogenetic and phylogeographic analysis of both isolates from T. evansi, suggest a complex evolutionary relationship with species of Trypanozoon subgenus. Moreover, the haplotype H2 was observed for the first time in Colombia, in common areas where human cases of T. evansi infection has been reported. These findings imply a relevant problem for animal health in the country, and highlight the importance of this infection in domestic animals and the possibility of human cases.
[Mh] Termos MeSH primário: Doenças do Cão/diagnóstico
Doenças do Cão/parasitologia
Trypanosoma/genética
Tripanossomíase/veterinária
[Mh] Termos MeSH secundário: Animais
Colômbia/epidemiologia
Diminazena/uso terapêutico
Doenças do Cão/tratamento farmacológico
Doenças do Cão/transmissão
Cães
Evolução Fatal
Geografia
Haplótipos
Masculino
Filogenia
Reação em Cadeia da Polimerase/veterinária
Tripanossomicidas/uso terapêutico
Trypanosoma/isolamento & purificação
Tripanossomíase/diagnóstico
Tripanossomíase/tratamento farmacológico
Tripanossomíase/transmissão
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Trypanocidal Agents); Y5G36EEA5Z (Diminazene)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170821
[Lr] Data última revisão:
170821
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170403
[St] Status:MEDLINE


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[PMID]:28288771
[Au] Autor:Tchamdja E; Kulo AE; Vitouley HS; Batawui K; Bankolé AA; Adomefa K; Cecchi G; Hoppenheit A; Clausen PH; De Deken R; Van Den Abbeele J; Marcotty T; Delespaux V
[Ad] Endereço:Direction de l'Elevage, BP 4041, Lomé, Togo.
[Ti] Título:Cattle breeding, trypanosomosis prevalence and drug resistance in Northern Togo.
[So] Source:Vet Parasitol;236:86-92, 2017 Mar 15.
[Is] ISSN:1873-2550
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:African Animal Trypanosomosis (AAT) is a major disease of cattle in Togo and its control is essentially based on chemotherapy. However, because of excessive use of trypanocides during the past decades, chemo-resistance in the parasites has developed. In order to assess the current situation of AAT and resistance to trypanocidal drugs in Northern Togo, a study was conducted on cattle from December 2012 to August 2013 in the regions of Kara and Savanes. An initial cross-sectional survey was carried out in 40 villages using the Haematocrit Centrifugation Technique (HCT). Out of these, 5 villages with a trypanosome prevalence of >10% were selected for a block treatment study (BT) with diminazene diaceturate (DA: 3.5mg/kg for a 14-day follow-up) and isometamidium chloride (ISM: 0.5mg/kg for a 28-day follow-up). Positive blood samples collected during the parasitological surveys and an equivalent number of negatives were further analyzed by PCR-RFLP for trypanosome species confirmation and molecular diagnosis of resistance to DA in Trypanosoma congolense. The results from 1883 bovine blood samples confirmed a high overall trypanosome prevalence of 10.8% in Northern Togo. PCR-RFLP revealed that T. congolense is the dominant pathogenic trypanosome species (50.5%) followed by T. vivax (27.3%), and T. brucei (16.2%). The BT showed varying levels of treatment failures ranging from 0 to 30% and from 0 to 50% for DA and for ISM respectively, suggesting the existence of resistant trypanosome populations in the study area. Our results show that AAT still represents a major obstacle to the development of cattle husbandry in Northern Togo. In areas of high AAT risk, a community-based integrated strategy combining vector control, rational use of trypanocidal drugs and improving the general condition of the animals is recommended to decision makers.
[Mh] Termos MeSH primário: Doenças dos Bovinos/prevenção & controle
Resistência a Medicamentos
Tripanossomicidas/farmacologia
Trypanosoma congolense/efeitos dos fármacos
Tripanossomíase Africana/veterinária
Tripanossomíase Bovina/parasitologia
[Mh] Termos MeSH secundário: Animais
Cruzamento
Bovinos
Doenças dos Bovinos/epidemiologia
Doenças dos Bovinos/parasitologia
Controle de Doenças Transmissíveis
Estudos Transversais
Diminazena/análogos & derivados
Diminazena/farmacologia
Fenantridinas/farmacologia
Prevalência
Togo/epidemiologia
Falha de Tratamento
Trypanosoma/efeitos dos fármacos
Tripanossomíase/epidemiologia
Tripanossomíase/parasitologia
Tripanossomíase/prevenção & controle
Tripanossomíase/veterinária
Tripanossomíase Africana/epidemiologia
Tripanossomíase Africana/parasitologia
Tripanossomíase Africana/prevenção & controle
Tripanossomíase Bovina/epidemiologia
Tripanossomíase Bovina/prevenção & controle
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Phenanthridines); 0 (Trypanocidal Agents); 7NH28I651F (isometamidium chloride); JI8SAD85NO (diminazene aceturate); Y5G36EEA5Z (Diminazene)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170908
[Lr] Data última revisão:
170908
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170315
[St] Status:MEDLINE


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[PMID]:28286324
[Au] Autor:Kamyingkird K; Cao S; Tuvshintulga B; Salama A; Mousa AA; Efstratiou A; Nishikawa Y; Yokoyama N; Igarashi I; Xuan X
[Ad] Endereço:National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, 080-8555, Japan; Department of Parasitology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok, 10900, Thailand.
[Ti] Título:Effects of dihydroorotate dehydrogenase (DHODH) inhibitors on the growth of Theileria equi and Babesia caballi in vitro.
[So] Source:Exp Parasitol;176:59-65, 2017 May.
[Is] ISSN:1090-2449
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Theileria equi and Babesia caballi are the causative agents of equine piroplasmosis (EP), which affects equine production in various parts of the world. However, a safe and effective drug is not currently available for treatment of EP. Dihydroorotate dehydrogenase (DHODH) is the fourth enzyme in the de novo pyrimidine synthesis pathway and has been known as a novel drug target for several apicomplexan protozoan parasites. In this study, we evaluated four DHODH inhibitors; atovaquone (ATV), leflunomide (LFN), brequinar (Breq), and 7-hydroxy-5-[1,2,4] triazolo [1,5,a] pyrimidine (TAZ) on the growth of T. equi and B. caballi in vitro and compared them to diminacene aceturate (Di) as the control drug. The growth of T. equi and B. caballi was significantly hindered by all inhibitors except TAZ. The half maximal inhibitory concentration (IC ) of ATV, LFN, Breq and Di against T. equi was approximately 0.028, 109, 11 and 40 µM, respectively, whereas the IC of ATV, LFN, Breq and Di against B. caballi was approximately 0.128, 193, 5.2 and 16.2 µM, respectively. Using bioinformatics and Western blot analysis, we showed that TeDHODH was similar to other Babesia parasite DHODHs, and confirmed that targeting DHODHs could be useful for the development of novel chemotherapeutics for treatment of EP.
[Mh] Termos MeSH primário: Babesia/efeitos dos fármacos
Inibidores Enzimáticos/farmacologia
Oxirredutases atuantes sobre Doadores de Grupo CH-CH/antagonistas & inibidores
Theileria/efeitos dos fármacos
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Antiprotozoários/farmacologia
Atovaquona/farmacologia
Babesia/classificação
Babesia/crescimento & desenvolvimento
Babesiose/tratamento farmacológico
Babesiose/parasitologia
Compostos de Bifenilo/farmacologia
Biologia Computacional
Diminazena/análogos & derivados
Diminazena/farmacologia
Inibidores Enzimáticos/uso terapêutico
Doenças dos Cavalos/tratamento farmacológico
Doenças dos Cavalos/parasitologia
Cavalos
Concentração Inibidora 50
Isoxazóis/farmacologia
Camundongos
Peso Molecular
Oxirredutases atuantes sobre Doadores de Grupo CH-CH/química
Filogenia
Plasmodium berghei/efeitos dos fármacos
Plasmodium berghei/crescimento & desenvolvimento
Pirimidinas/farmacologia
Pirimidinas/uso terapêutico
Theileria/classificação
Theileria/crescimento & desenvolvimento
Theileriose/tratamento farmacológico
Theileriose/parasitologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antiprotozoal Agents); 0 (Biphenyl Compounds); 0 (Enzyme Inhibitors); 0 (Isoxazoles); 0 (Pyrimidines); 5XL19F49H6 (brequinar); EC 1.3.- (Oxidoreductases Acting on CH-CH Group Donors); EC 1.3.5.2 (dihydroorotate dehydrogenase); G162GK9U4W (leflunomide); JI8SAD85NO (diminazene aceturate); K8CXK5Q32L (pyrimidine); Y5G36EEA5Z (Diminazene); Y883P1Z2LT (Atovaquone)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170411
[Lr] Data última revisão:
170411
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170314
[St] Status:MEDLINE


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[PMID]:28194625
[Au] Autor:Cossic BGA; Adjahoutonon B; Gloaguen P; Dibanganga GL; Maganga G; Leroy P; MacLeod ET; Picozzi K
[Ad] Endereço:Division of Infection and Pathway Medicine, Edinburgh Medical School: Biomedical Sciences, University of Edinburgh, Edinburgh, UK.
[Ti] Título:Trypanosomiasis challenge estimation using the diminazene aceturate (Berenil) index in Zebu in Gabon.
[So] Source:Trop Anim Health Prod;49(3):619-624, 2017 Mar.
[Is] ISSN:1573-7438
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A longitudinal study was conducted within a cattle ranch in Gabon to determine the diminazene aceturate (Berenil) index (DAI) in a group of Zebu, raised under low tsetse density; this measure providing an assessment of trypanosomiasis risk. The objective was to evaluate the trypanosomiasis pressure thus informing trypanosomiasis control methods and cattle management. Twenty female adult Zebu were monitored for 24 weeks during the dry season. Blood samples were collected on a weekly basis and subjected to parasitological and haematological analysis (n = 480), using the buffy-coat method and the packed cell volume value (PCV), respectively, infected animals were treated with a single intramuscular injection of diminazene aceturate (8 mg/kg). Twenty-nine single infectious events were recorded and a DAI of 1.45 was calculated. Two trypanosome species were identified: Trypanosoma congolense (96.2%) and Trypanosoma vivax (3.8%). The mean PCV value of the infected animals was lower (26.6) compared to non-infected animals (32.0). This study shows that DAI may be a useful tool to assess trypanosomiasis. However, this is a time-consuming method that may be improved by using randomly selected sentinel animals to adapt the chemoprophylactic schemes, hence decreasing the costs and the drug resistance risk.
[Mh] Termos MeSH primário: Diminazena/análogos & derivados
Resistência a Medicamentos
Tripanossomicidas/uso terapêutico
Tripanossomíase Bovina/epidemiologia
[Mh] Termos MeSH secundário: Animais
Bovinos
Diminazena/uso terapêutico
Feminino
Gabão/epidemiologia
Estudos Longitudinais
Chuvas
Estações do Ano
Clima Tropical
Tripanossomíase Africana/epidemiologia
Tripanossomíase Africana/veterinária
Tripanossomíase Bovina/tratamento farmacológico
Tripanossomíase Bovina/parasitologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Trypanocidal Agents); JI8SAD85NO (diminazene aceturate); Y5G36EEA5Z (Diminazene)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171019
[Lr] Data última revisão:
171019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170215
[St] Status:MEDLINE
[do] DOI:10.1007/s11250-017-1239-2


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[PMID]:28099874
[Au] Autor:Waema MW; Maina NW; Ngotho M; Karanja SM; Gachie BM; Maranga DN; Kagira JM
[Ad] Endereço:Jomo Kenyatta University of Agriculture and Technology, College of Health Sciences, Biochemistry Department, P.O. Box 62000-00200, Nairobi, Kenya.
[Ti] Título:IgM, lgG and IL-6 profiles in the Trypanosoma brucei brucei monkey model of human African trypanosomiasis.
[So] Source:Acta Trop;168:45-49, 2017 Apr.
[Is] ISSN:1873-6254
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Human African trypanosomiasis (HAT) patients manifest immunological profiles, whose variations over time can be used to indicate disease progression. However, monitoring of these biomarkers in human patients is beset by several limitations which can be offset by using chronic animal models. A recent improved monkey model of HAT using a Trypanosoma brucei brucei isolate has been developed but the immunological profile has not been elucidated. The objectives of the current study was to determine the IgM, IgG and IL-6 profiles in blood and cerebrospinal fluid (CSF) in vervet monkeys infected with T. b. brucei. Three vervet monkeys were infected intravenously with 10 T. b. brucei, monitored for disease development and subsequently treated 28days post infection (dpi) sub-curatively using diminazene aceturate (DA) to induce late stage disease and curatively treated with melarsoprol (Mel B) at 119 dpi, respectively. Matched serum and cerebrospinal fluid (CSF) samples were obtained at regular intervals and immunospecific IgM, immunoglobulin G (IgG) were quantified by ELISA while IL-6 was assayed using a cytometric bead array (CBA) kit. Results showed that following infection, CSF IgM, IgG, IL-6 and serum IL-6 were significantly (p<0.05) elevated with peak levels coinciding with relapse parasitaemia. The IgG levels increased to reach OD peak levels of 0.442±0.5 at 126 dpi. After curative treatment with MelB, the serum IgM and Ig G levels fell rapidly to attain pre-infection levels within 35 and 49days, respectively. This shows that the profile of these immunoglobulins can be used as an indicator of curative treatment. CSF IL-6 concentrations of infected vervet monkeys showed no significant change (P>0.05) between infection and 35 dpi but levels increased significantly (P<0.05) with the highest level of 55.53pg/ml recorded at112 dpi. IL-6 elevation from 35 dpi may be indicative of parasite neuroinvasion hence can be used as possible candidate marker for late stage disease in the monkey model. Further, the marker can also be used in conjunction with IgG and IgM as markers for development of test of cure for HAT.
[Mh] Termos MeSH primário: Imunoglobulina G/análise
Imunoglobulina M/análise
Interleucina-6/sangue
Tripanossomíase Africana/imunologia
Tripanossomíase Africana/parasitologia
[Mh] Termos MeSH secundário: Animais
Antígenos de Protozoários/imunologia
Cercopithecus aethiops
Diminazena/análogos & derivados
Modelos Animais de Doenças
Progressão da Doença
Ensaio de Imunoadsorção Enzimática
Imunoglobulina G/imunologia
Imunoglobulina M/imunologia
Interleucina-6/imunologia
Trypanosoma brucei brucei/imunologia
Tripanossomíase Africana/sangue
Tripanossomíase Africana/líquido cefalorraquidiano
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, Protozoan); 0 (Immunoglobulin G); 0 (Immunoglobulin M); 0 (Interleukin-6); JI8SAD85NO (diminazene aceturate); Y5G36EEA5Z (Diminazene)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170707
[Lr] Data última revisão:
170707
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170119
[St] Status:MEDLINE


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[PMID]:27650882
[Au] Autor:Ademola IO; Odeniran PO
[Ad] Endereço:a Department of Veterinary Microbiology and Parasitology, Faculty of Veterinary Medicine , University of Ibadan , Ibadan , Oyo , Nigeria.
[Ti] Título:Novel trypanocide from an extract of Pleurotus sajor-caju against Trypanosoma congolense.
[So] Source:Pharm Biol;55(1):132-138, 2017 Dec.
[Is] ISSN:1744-5116
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:CONTEXT: Control of African trypanosomiasis relies on chemotherapy, but the development of resistance and the problem of drug residues require research for alternatives. Triterpenes and phenolics, the major constituents of Pleurotus sajor-caju (Fr.) Singer (Pleurotaceae), are reported to be effective against trypanosomiasis. OBJECTIVE: Trypanocidal effect of whole Pleurotus sajor-caju aqueous extract was investigated in vivo against Trypanosoma congolense. MATERIALS AND METHODS: Mice (25-32 g) were divided into seven groups of six animals. Mice in groups A-F received 2.5 × 10 trypanosomes, while group G was uninfected. Extracts (100-250 mg/kg) were administered intraperitoneally for 5 days to groups A-D while diminazine aceturate (group E) and normal saline (group F) served as positive and negative controls, respectively. Parasitemia, survival time, body weight and haematological parameters were monitored for 60 days post-treatment. RESULTS: Parasitemia decreased significantly (p < 0.01) post-treatment with 200 and 250 mg/kg of the extract and became undetectable by day 16 and 12 post-infection, respectively; the ED was 221.5 mg/kg. The packed cell volume (PCV) and the weight of mice treated with 250 mg/kg extract were 46.20 ± 2.6% and 32.05 ± 3.63 g, respectively, which is higher than the group treated with diminazine aceturate. The mean survival time of animals in groups D and E was >60 days, while that of group F was <4 days. Differential leucocyte count on day 68 post-infection in groups C, D and E were not significantly different. CONCLUSION: Pleurotus sajor-caju therefore could be a potential source of new trypanocidal drugs.
[Mh] Termos MeSH primário: Pleurotus/química
Tripanossomicidas/farmacologia
Trypanosoma congolense/efeitos dos fármacos
Tripanossomíase Africana/tratamento farmacológico
[Mh] Termos MeSH secundário: Animais
Biomarcadores/sangue
Peso Corporal/efeitos dos fármacos
Diminazena/análogos & derivados
Diminazena/farmacologia
Modelos Animais de Doenças
Relação Dose-Resposta a Droga
Camundongos Endogâmicos BALB C
Parasitemia/tratamento farmacológico
Parasitemia/parasitologia
Fatores de Tempo
Tripanossomicidas/isolamento & purificação
Tripanossomíase Africana/sangue
Tripanossomíase Africana/parasitologia
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 0 (Trypanocidal Agents); JI8SAD85NO (diminazene aceturate); Y5G36EEA5Z (Diminazene)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170309
[Lr] Data última revisão:
170309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160922
[St] Status:MEDLINE


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Caliari, Marcelo V
[PMID]:27550926
[Au] Autor:Prata LO; Rodrigues CR; Martins JM; Vasconcelos PC; Oliveira FM; Ferreira AJ; Rodrigues-Machado MD; Caliari MV
[Ad] Endereço:Departamento de Patologia Geral, Instituto de Ciências Biológicas da Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, CEP 31 270-901, Brasil.
[Ti] Título:Original Research: ACE2 activator associated with physical exercise potentiates the reduction of pulmonary fibrosis.
[So] Source:Exp Biol Med (Maywood);242(1):8-21, 2017 Jan.
[Is] ISSN:1535-3699
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The interstitial lung diseases are poorly understood and there are currently no studies evaluating the association of physical exercise with an ACE2 activator (DIZE) as a possible treatment for this group of diseases. We evaluate the effects of pharmacological treatment with an angiotensin-converting enzyme 2 activator drug, associated with exercise, on the pulmonary lesions induced by bleomycin. From the 96 male Balb/c mice used in the experiment, only 49 received 8 U/kg of bleomycin (BLM, intratracheally). The mice were divided into control (C) and bleomycin (BLM) groups, sedentary and trained (C-SED, C-EXE, BLM-SED, BLM-EXE), control and bleomycin and also sedentary and trained treated with diminazene (C-SED/E, C-EXE/E, BLM-SED/E, BLM-EXE/E). The animals were trained five days/week, 1 h/day with 60% of the maximum load obtained in a functional capacity test, for four weeks. Diminazene groups were treated (1 mg/kg, by gavage) daily until the end of the experiment. The lungs were collected 48 h after the training program, set in buffered formalin and investigated by Gomori's trichrome, immunohistochemistry of collagen type I, TGF-ß , beta-prolyl-4-hydroxylase, MMP-1 and -2. The BLM-EXE/E group obtained a significant increase in functional capacity, reduced amount of fibrosis and type I collagen, decreased expression of TGF-ß and beta-prolyl-4-hydroxylase and an increase of metalloproteinase -1, -2 when compared with the other groups. The present research shows, for the first time, that exercise training associated with the activation of ACE2 potentially reduces pulmonary fibrosis.
[Mh] Termos MeSH primário: Diminazena/farmacologia
Peptidil Dipeptidase A/metabolismo
Condicionamento Físico Animal/fisiologia
Fibrose Pulmonar/terapia
[Mh] Termos MeSH secundário: Animais
Colágeno Tipo I/metabolismo
Modelos Animais de Doenças
Pulmão/efeitos dos fármacos
Pulmão/metabolismo
Pulmão/patologia
Masculino
Metaloproteinase 1 da Matriz/metabolismo
Metaloproteinase 2 da Matriz/metabolismo
Camundongos Endogâmicos BALB C
Resistência Física/efeitos dos fármacos
Fibrose Pulmonar/tratamento farmacológico
Fibrose Pulmonar/fisiopatologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Collagen Type I); EC 3.4.15.1 (Peptidyl-Dipeptidase A); EC 3.4.17.- (angiotensin converting enzyme 2); EC 3.4.24.24 (Matrix Metalloproteinase 2); EC 3.4.24.7 (Matrix Metalloproteinase 1); Y5G36EEA5Z (Diminazene)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170810
[Lr] Data última revisão:
170810
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160824
[St] Status:MEDLINE



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