Base de dados : MEDLINE
Pesquisa : D02.078.766 [Categoria DeCS]
Referências encontradas : 1479 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 148 ir para página                         

  1 / 1479 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28366470
[Au] Autor:Saito H; Katagiri A; Okada S; Mikuzuki L; Kubo A; Suzuki T; Ohara K; Lee J; Gionhaku N; Iinuma T; Bereiter DA; Iwata K
[Ad] Endereço:Department of Complete Denture Prosthodontics, Nihon University School of Dentistry, 1-8-13 Kandasurugadai, Chiyoda-ku, Tokyo 101-8310, Japan; Department of Physiology, Nihon University School of Dentistry, 1-8-13 Kandasurugadai, Chiyoda-ku, Tokyo 101-8310, Japan. Electronic address: dehi14017@g.nih
[Ti] Título:Ascending projections of nociceptive neurons from trigeminal subnucleus caudalis: A population approach.
[So] Source:Exp Neurol;293:124-136, 2017 Jul.
[Is] ISSN:1090-2430
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Second-order neurons in trigeminal subnucleus caudalis (Vc) and upper cervical spinal cord (C1) are critical for craniofacial pain processing and project rostrally to terminate in: ventral posteromedial thalamic nucleus (VPM), medial thalamic nuclei (MTN) and parabrachial nuclei (PBN). The contribution of each region to trigeminal nociception was assessed by the number of phosphorylated extracellular signal-regulated kinase-immunoreactive (pERK-IR) neurons co-labeled with fluorogold (FG). The phenotype of pERK-IR neurons was further defined by the expression of neurokinin 1 receptor (NK1). The retrograde tracer FG was injected into VPM, MTN or PBN of the right hemisphere and after seven days, capsaicin was injected into the left upper lip in male rats. Nearly all pERK-IR neurons were found in superficial laminae of Vc-C1 ipsilateral to the capsaicin injection. Nearly all VPM and MTN FG-labeled neurons in Vc-C1 were found contralateral to the injection site, whereas FG-labeled neurons were found bilaterally after PBN injection. The percentage of FG-pERK-NK1-IR neurons was significantly greater (>10%) for PBN projection neurons than for VPM and MTN projection neurons (<3%). pERK-NK1-IR VPM projection neurons were found mainly in the middle-Vc, while pERK-NK1-immunoreactive MTN or PBN projection neurons were found in the middle-Vc and caudal Vc-C1. These results suggest that a significant percentage of capsaicin-responsive neurons in superficial laminae of Vc-C1 project directly to PBN, while neurons that project to VPM and MTN are subject to greater modulation by pERK-IR local interneurons. Furthermore, the rostrocaudal distribution differences of FG-pERK-NK1-IR neurons in Vc-C1 may reflect functional differences between these projection areas regarding craniofacial pain.
[Mh] Termos MeSH primário: Dor Facial/patologia
Nociceptores/patologia
Núcleos do Trigêmeo/patologia
[Mh] Termos MeSH secundário: Animais
Capsaicina/toxicidade
Modelos Animais de Doenças
MAP Quinases Reguladas por Sinal Extracelular/metabolismo
Dor Facial/induzido quimicamente
Masculino
Núcleo Mediodorsal do Tálamo/patologia
Vias Neurais/patologia
Vias Neurais/fisiologia
Nociceptores/metabolismo
Núcleos Parabraquiais/patologia
Ratos
Ratos Sprague-Dawley
Receptores da Neurocinina-1/metabolismo
Fármacos do Sistema Sensorial/toxicidade
Estatísticas não Paramétricas
Estilbamidinas/metabolismo
Núcleos Ventrais do Tálamo/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (2-hydroxy-4,4'-diamidinostilbene, methanesulfonate salt); 0 (Receptors, Neurokinin-1); 0 (Sensory System Agents); 0 (Stilbamidines); EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases); S07O44R1ZM (Capsaicin)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170404
[St] Status:MEDLINE


  2 / 1479 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28007991
[Au] Autor:Valencia Garcia S; Libourel PA; Lazarus M; Grassi D; Luppi PH; Fort P
[Ad] Endereço:Neuroscience Research Center of Lyon (CRNL), CNRS UMR 5292, INSERM U1028, SLEEP Team, Lyon, France.
[Ti] Título:Genetic inactivation of glutamate neurons in the rat sublaterodorsal tegmental nucleus recapitulates REM sleep behaviour disorder.
[So] Source:Brain;140(2):414-428, 2017 02.
[Is] ISSN:1460-2156
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:SEE SCHENCK AND MAHOWALD DOI101093/AWW329 FOR A SCIENTIFIC COMMENTARY ON THIS ARTICLE: Idiopathic REM sleep behaviour disorder is characterized by the enactment of violent dreams during paradoxical (REM) sleep in the absence of normal muscle atonia. Accumulating clinical and experimental data suggest that REM sleep behaviour disorder might be due to the neurodegeneration of glutamate neurons involved in paradoxical sleep and located within the pontine sublaterodorsal tegmental nucleus. The purpose of the present work was thus to functionally determine first, the role of glutamate sublaterodorsal tegmental nucleus neurons in paradoxical sleep and second, whether their genetic inactivation is sufficient for recapitulating REM sleep behaviour disorder in rats. For this goal, we first injected two retrograde tracers in the intralaminar thalamus and ventral medulla to disentangle neuronal circuits in which sublaterodorsal tegmental nucleus is involved; second we infused bilaterally in sublaterodorsal tegmental nucleus adeno-associated viruses carrying short hairpin RNAs targeting Slc17a6 mRNA [which encodes vesicular glutamate transporter 2 (vGluT2)] to chronically impair glutamate synaptic transmission in sublaterodorsal tegmental nucleus neurons. At the neuroanatomical level, sublaterodorsal tegmental nucleus neurons specifically activated during paradoxical sleep hypersomnia send descending efferents to glycine/GABA neurons within the ventral medulla, but not ascending projections to the intralaminar thalamus. These data suggest a crucial role of sublaterodorsal tegmental nucleus neurons rather in muscle atonia than in paradoxical sleep generation. In line with this hypothesis, 30 days after adeno-associated virus injections into sublaterodorsal tegmental nucleus rats display a decrease of 30% of paradoxical sleep daily quantities, and a significant increase of muscle tone during paradoxical sleep concomitant to a tremendous increase of abnormal motor dream-enacting behaviours. These animals display symptoms and behaviours during paradoxical sleep that closely mimic human REM sleep behaviour disorder. Altogether, our data demonstrate that glutamate sublaterodorsal tegmental nucleus neurons generate muscle atonia during paradoxical sleep likely through descending projections to glycine/GABA premotor neurons in the ventral medulla. Although playing a role in paradoxical sleep regulation, they are, however, not necessary for inducing the state itself. The present work further validates a potent new preclinical REM sleep behaviour disorder model that opens avenues for studying and treating this disabling sleep disorder, and advances potential regions implicated in prodromal stages of synucleinopathies such as Parkinson's disease.
[Mh] Termos MeSH primário: Ácido Glutâmico/metabolismo
Neurônios/fisiologia
Área Pré-Tetal/patologia
Transtorno do Comportamento do Sono REM/patologia
[Mh] Termos MeSH secundário: Animais
Contagem de Células
Toxina da Cólera/farmacocinética
Dependovirus/genética
Modelos Animais de Doenças
Transportador 5 de Aminoácido Excitatório/genética
Transportador 5 de Aminoácido Excitatório/metabolismo
Regulação da Expressão Gênica/genética
Proteínas da Membrana Plasmática de Transporte de Glicina/genética
Proteínas da Membrana Plasmática de Transporte de Glicina/metabolismo
Masculino
Área Pré-Tetal/metabolismo
Proteínas Proto-Oncogênicas c-fos/metabolismo
Transtorno do Comportamento do Sono REM/etiologia
RNA Mensageiro/metabolismo
RNA Interferente Pequeno/genética
RNA Interferente Pequeno/metabolismo
Ratos
Ratos Sprague-Dawley
Privação do Sono/complicações
Análise Espectral
Estilbamidinas/farmacocinética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (2-hydroxy-4,4'-diamidinostilbene, methanesulfonate salt); 0 (Excitatory Amino Acid Transporter 5); 0 (Glycine Plasma Membrane Transport Proteins); 0 (Proto-Oncogene Proteins c-fos); 0 (RNA, Messenger); 0 (RNA, Small Interfering); 0 (Slc6a5 protein, rat); 0 (Stilbamidines); 3KX376GY7L (Glutamic Acid); 9012-63-9 (Cholera Toxin)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:161224
[St] Status:MEDLINE
[do] DOI:10.1093/brain/aww310


  3 / 1479 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27539453
[Au] Autor:Fillinger C; Yalcin I; Barrot M; Veinante P
[Ad] Endereço:Centre National de la Recherche Scientifique, Institut des Neurosciences Cellulaires et Intégratives, 5 rue Blaise Pascal, 67084, Strasbourg, France.
[Ti] Título:Afferents to anterior cingulate areas 24a and 24b and midcingulate areas 24a' and 24b' in the mouse.
[So] Source:Brain Struct Funct;222(3):1509-1532, 2017 Apr.
[Is] ISSN:1863-2661
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Areas 24a and 24b of the anterior cingulate cortex (ACC) play a major role in cognition, emotion and pain. While their connectivity has been studied in primate and in rat, a complete mapping was still missing in the mouse. Here, we analyzed the afferents to the mouse ACC by injecting retrograde tracers in the ventral and dorsal areas of the ACC (areas 24a/b) and of the midcingulate cortex (MCC; areas 24a'/b'). Our results reveal inputs from five principal groups of structures: (1) cortical areas, mainly the orbital, medial prefrontal, retrosplenial, parietal associative, primary and secondary sensory areas and the hippocampus, (2) basal forebrain, mainly the basolateral amygdaloid nucleus, the claustrum and the horizontal limb of the diagonal band of Broca, (3) the thalamus, mainly the anteromedial, lateral mediodorsal, ventromedial, centrolateral, central medial and reuniens/rhomboid nuclei, (4) the hypothalamus, mainly the lateral and retromammillary areas, and (5) the brainstem, mainly the monoaminergic centers. The neurochemical nature of inputs from the diagonal band of Broca and brainstem centers was also investigated by double-labeling, showing that only a part of these afferents were cholinergic or monoaminergic. Comparisons between the areas indicate that areas 24a and 24b receive qualitatively similar inputs, but with different densities. These differences are more pronounced when comparing the inputs to ACC's areas 24a/24b to the inputs to MCC's areas 24a'/24b'. These results provide a complete analysis of the afferents to the mouse areas 24a/24b and 24a'/24b', which shows important similarity with the connectivity of homologous areas in rats, and brings the anatomical basis necessary to address the roles of cingulate areas in mice.
[Mh] Termos MeSH primário: Mapeamento Encefálico
Giro do Cíngulo/anatomia & histologia
Rede Nervosa/fisiologia
Vias Neurais/fisiologia
[Mh] Termos MeSH secundário: Animais
Mapeamento Encefálico/métodos
Córtex Cerebral/citologia
Córtex Cerebral/fisiologia
Toxina da Cólera/metabolismo
Colina O-Acetiltransferase/metabolismo
Masculino
Camundongos
Camundongos Endogâmicos C57BL
Serotonina/metabolismo
Estilbamidinas/metabolismo
Tirosina 3-Mono-Oxigenase
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (2-hydroxy-4,4'-diamidinostilbene, methanesulfonate salt); 0 (Stilbamidines); 333DO1RDJY (Serotonin); 9012-63-9 (Cholera Toxin); EC 1.14.16.2 (Tyrosine 3-Monooxygenase); EC 2.3.1.6 (Choline O-Acetyltransferase)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160820
[St] Status:MEDLINE
[do] DOI:10.1007/s00429-016-1290-1


  4 / 1479 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27539452
[Au] Autor:Billwiller F; Renouard L; Clement O; Fort P; Luppi PH
[Ad] Endereço:UMR 5292 CNRS/U1028 INSERM, Faculté de Médecine RTH Laennec, Centre de Recherche en Neurosciences de Lyon (CRNL), SLEEP Team, Université Claude Bernard Lyon I, 7 Rue Guillaume Paradin, 69372, Lyon Cedex 08, France.
[Ti] Título:Differential origin of the activation of dorsal and ventral dentate gyrus granule cells during paradoxical (REM) sleep in the rat.
[So] Source:Brain Struct Funct;222(3):1495-1507, 2017 Apr.
[Is] ISSN:1863-2661
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:We recently demonstrated that granule cells located in the dorsal dentate gyrus (dDG) are activated by neurons located in the lateral supramammillary nucleus (SumL) during paradoxical sleep (PS) hypersomnia. To determine whether these neurons are glutamatergic and/or GABAergic, we combined FOS immunostaining with in situ hybridization of vesicular glutamate transporter 2 (vGLUT2, a marker of glutamatergic neurons) or that of the vesicular GABA transporter (vGAT, a marker of GABAergic neurons) mRNA in rats displaying PS hypersomnia (PSR). We found that 84 and 76 % of the FOS+ SumL neurons in PSR rats expressed vGLUT2 and vGAT mRNA, respectively. Then, we examined vGLUT2 and FOS immunostaining in the dorsal and ventral DG of PSR rats with a neurochemical lesion of the Sum. In PSR-lesioned animals but not in sham animals, nearly all vGLUT2+ fibers and FOS+ neurons disappeared in the dDG, but not in the ventral DG (vDG). To identify the pathway (s) responsible (s) for the activation of the vDG during PS hypersomnia, we combined Fluorogold (FG) injection in the vDG of PSR rats with FOS staining. We found a large number of neurons FOS-FG+, specifically in the medial entorhinal cortex (ENTm). Altogether, our results suggest that SumL neurons with a unique dual glutamatergic and GABAergic phenotype are responsible for the activation of the dDG during PS hypersomnia, while vDG granule neurons are activated by ENTm cortical neurons. These results suggest differential mechanisms and functions for the activation of the dDG and the vDG granule cells during PS.
[Mh] Termos MeSH primário: Giro Denteado/citologia
Neurônios/fisiologia
Sono REM/fisiologia
[Mh] Termos MeSH secundário: Animais
Contagem de Células
Giro Denteado/lesões
Eletroencefalografia
Eletromiografia
Hipotálamo Posterior/citologia
Masculino
Proteínas Oncogênicas v-fos/genética
Proteínas Oncogênicas v-fos/metabolismo
Fosfopiruvato Hidratase/metabolismo
Ratos
Ratos Sprague-Dawley
Privação do Sono
Estatísticas não Paramétricas
Estilbamidinas/metabolismo
Proteína Vesicular 2 de Transporte de Glutamato/genética
Proteína Vesicular 2 de Transporte de Glutamato/metabolismo
Vigília
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (2-hydroxy-4,4'-diamidinostilbene, methanesulfonate salt); 0 (Oncogene Proteins v-fos); 0 (Slc17a6 protein, rat); 0 (Stilbamidines); 0 (Vesicular Glutamate Transport Protein 2); EC 4.2.1.11 (Phosphopyruvate Hydratase)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160820
[St] Status:MEDLINE
[do] DOI:10.1007/s00429-016-1289-7


  5 / 1479 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27396693
[Au] Autor:Gupta RG; Schafer C; Ramaroson Y; Sciullo MG; Horn CC
[Ad] Endereço:University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.
[Ti] Título:Role of the abdominal vagus and hindbrain in inhalational anesthesia-induced vomiting.
[So] Source:Auton Neurosci;202:114-121, 2017 Jan.
[Is] ISSN:1872-7484
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The incidence of postoperative nausea and vomiting (PONV) can be as high as 80% in patients with risk factors (e.g., females, history of motion sickness). PONV delays postoperative recovery and costs several hundred million dollars annually. Cell-based assays show that halogenated ethers (e.g., isoflurane) activate 5-HT receptors, which are found on gastrointestinal vagal afferents and in the hindbrain - key pathways for producing nausea and vomiting. This project evaluated the role of the vagus and activation of the hindbrain in isoflurane-induced emesis in musk shrews, a small animal model with a vomiting reflex, which is lacking in rats and mice. Sham-operated and abdominal vagotomized shrews were exposed to 1 to 3% isoflurane to determine effects on emesis; vagotomy was confirmed by lack of vagal transport of the neuronal tracer Fluoro-Gold. In an additional study, shrews were exposed to isoflurane and hindbrain c-Fos was measured at 90min after exposure using immunohistochemistry. There were no statistically significant effects of vagotomy on isoflurane-induced emesis compared to sham-operated controls. Isoflurane exposure produced a significant increase in c-Fos-positive cells in the nucleus of the solitary tract and vestibular nuclei but not in the area postrema or dorsal motor nucleus. These results indicate that the abdominal vagus plays no role in isoflurane-induced emesis and suggest that isoflurane activates emesis by action on the hindbrain, as shown by c-Fos labeling. Ultimately, knowledge of the mechanisms of inhalational anesthesia-induced PONV could lead to more targeted therapies to control PONV.
[Mh] Termos MeSH primário: Anestésicos Inalatórios/efeitos adversos
Rombencéfalo/efeitos dos fármacos
Nervo Vago/efeitos dos fármacos
Vômito/induzido quimicamente
[Mh] Termos MeSH secundário: Anestesia por Inalação/efeitos adversos
Anestésicos Inalatórios/farmacologia
Animais
Relação Dose-Resposta a Droga
Eméticos/farmacologia
Feminino
Imuno-Histoquímica
Isoflurano/efeitos adversos
Isoflurano/farmacologia
Modelos Animais
Vias Neurais/efeitos dos fármacos
Vias Neurais/patologia
Vias Neurais/fisiopatologia
Proteínas Proto-Oncogênicas c-fos/metabolismo
Distribuição Aleatória
Rombencéfalo/patologia
Rombencéfalo/fisiopatologia
Musaranhos
Estilbamidinas
Vagotomia
Nervo Vago/patologia
Nervo Vago/fisiopatologia
Vômito/patologia
Vômito/fisiopatologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (2-hydroxy-4,4'-diamidinostilbene, methanesulfonate salt); 0 (Anesthetics, Inhalation); 0 (Emetics); 0 (Proto-Oncogene Proteins c-fos); 0 (Stilbamidines); CYS9AKD70P (Isoflurane)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170821
[Lr] Data última revisão:
170821
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160712
[St] Status:MEDLINE


  6 / 1479 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
[PMID]:27685774
[Au] Autor:Ono H; Imai H; Miyawaki S; Nakatomi H; Saito N
[Ad] Endereço:Department of Neurosurgery, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
[Ti] Título:Rat white matter injury model induced by endothelin-1 injection: technical modification and pathological evaluation.
[So] Source:Acta Neurobiol Exp (Wars);76(3):212-24, 2016.
[Is] ISSN:1689-0035
[Cp] País de publicação:Poland
[La] Idioma:eng
[Ab] Resumo:White matter injury is an important cause of functional disability of the brain. We comprehensively analyzed a modified endothelin-1 (ET­1) injection-induced white matter injury model in the rat which is very valuable for investigating the underlying mechanisms of subcortical ischemic stroke. ET-1 was stereotactically injected into the internal capsule of the rat. To avoid complications with leakage of ET-1 into the lateral ventricle, the safest trajectory angle to the target was established. Rats with white matter injury were extensively evaluated for structural changes and functional sequelae, using motor function tests, magnetic resonance (MR) imaging, histopathology evolution, volume estimation of the lesion, and neuroanatomical identification of affected neurons using the retrograde tracer hydroxystilbamidine. Optimization of the trajectory of the ET-1 injection needle provided excellent survival rate. MR imaging visualized the white matter injury 2 days after surgery. Motor function deficit appeared temporarily after the operation. Histological studies confirmed damage of axons and myelin sheaths followed by inflammatory reaction and gliosis similar to lacunar infarction, with lesion volume of less than 1% of the whole brain. Hydroxystilbamidine injected into the lesion revealed wide spatial distribution of the affected neuronal population. Compared with prior ET-1 injection models, this method induced standardized amount of white matter damage and temporary motor function deficit in a reproducible and safe manner. The present model is valuable for studying the pathophysiology of not only ischemia, but a broader set of white matter damage conditions in the lissencephalic brain.
[Mh] Termos MeSH primário: Modelos Animais de Doenças
Endotelina-1/toxicidade
Leucoencefalopatias/induzido quimicamente
Leucoencefalopatias/patologia
[Mh] Termos MeSH secundário: Precursor de Proteína beta-Amiloide/metabolismo
Animais
Ectodisplasinas/metabolismo
Lateralidade Funcional/efeitos dos fármacos
Proteína Glial Fibrilar Ácida/metabolismo
Cápsula Interna/efeitos dos fármacos
Leucoencefalopatias/diagnóstico por imagem
Leucoencefalopatias/fisiopatologia
Locomoção/efeitos dos fármacos
Locomoção/fisiologia
Imagem por Ressonância Magnética
Exame Neurológico
Desempenho Psicomotor/efeitos dos fármacos
Desempenho Psicomotor/fisiologia
Ratos
Ratos Sprague-Dawley
Estilbamidinas/farmacocinética
Natação/psicologia
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (2-hydroxy-4,4'-diamidinostilbene, methanesulfonate salt); 0 (Amyloid beta-Protein Precursor); 0 (Ectodysplasins); 0 (Endothelin-1); 0 (Glial Fibrillary Acidic Protein); 0 (Stilbamidines)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170209
[Lr] Data última revisão:
170209
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160930
[St] Status:MEDLINE


  7 / 1479 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27655966
[Au] Autor:Fischl MJ; Burger RM; Schmidt-Pauly M; Alexandrova O; Sinclair JL; Grothe B; Forsythe ID; Kopp-Scheinpflug C
[Ad] Endereço:Division of Neurobiology, Department of Biology II, Ludwig Maximilian University Munich, Planegg-Martinsried, Germany.
[Ti] Título:Physiology and anatomy of neurons in the medial superior olive of the mouse.
[So] Source:J Neurophysiol;116(6):2676-2688, 2016 Dec 01.
[Is] ISSN:1522-1598
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In mammals with good low-frequency hearing, the medial superior olive (MSO) computes sound location by comparing differences in the arrival time of a sound at each ear, called interaural time disparities (ITDs). Low-frequency sounds are not reflected by the head, and therefore level differences and spectral cues are minimal or absent, leaving ITDs as the only cue for sound localization. Although mammals with high-frequency hearing and small heads (e.g., bats, mice) barely experience ITDs, the MSO is still present in these animals. Yet, aside from studies in specialized bats, in which the MSO appears to serve functions other than ITD processing, it has not been studied in small mammals that do not hear low frequencies. Here we describe neurons in the mouse brain stem that share prominent anatomical, morphological, and physiological properties with the MSO in species known to use ITDs for sound localization. However, these neurons also deviate in some important aspects from the typical MSO, including a less refined arrangement of cell bodies, dendrites, and synaptic inputs. In vitro, the vast majority of neurons exhibited a single, onset action potential in response to suprathreshold depolarization. This spiking pattern is typical of MSO neurons in other species and is generated from a complement of K 1, K 3, and I currents. In vivo, mouse MSO neurons show bilateral excitatory and inhibitory tuning as well as an improvement in temporal acuity of spiking during bilateral acoustic stimulation. The combination of classical MSO features like those observed in gerbils with more unique features similar to those observed in bats and opossums make the mouse MSO an interesting model for exploiting genetic tools to test hypotheses about the molecular mechanisms and evolution of ITD processing.
[Mh] Termos MeSH primário: Potenciais de Ação/fisiologia
Neurônios/fisiologia
Complexo Olivar Superior/citologia
Complexo Olivar Superior/metabolismo
[Mh] Termos MeSH secundário: Estimulação Acústica
Animais
Animais Recém-Nascidos
Vias Auditivas/fisiologia
Colina O-Acetiltransferase/metabolismo
Estimulação Elétrica
Proteínas da Membrana Plasmática de Transporte de Glicina/metabolismo
Técnicas In Vitro
Camundongos
Camundongos Endogâmicos C57BL
Proteínas Associadas aos Microtúbulos/metabolismo
Modelos Neurológicos
Neurônios/metabolismo
Técnicas de Patch-Clamp
Fosfopiruvato Hidratase/metabolismo
Psicoacústica
Estilbamidinas/farmacocinética
Proteína Vesicular 1 de Transporte de Glutamato/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (2-hydroxy-4,4'-diamidinostilbene, methanesulfonate salt); 0 (Glycine Plasma Membrane Transport Proteins); 0 (Microtubule-Associated Proteins); 0 (Slc6a5 protein, mouse); 0 (Stilbamidines); 0 (Vesicular Glutamate Transport Protein 1); EC 2.3.1.6 (Choline O-Acetyltransferase); EC 4.2.1.11 (Phosphopyruvate Hydratase)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160923
[St] Status:MEDLINE
[do] DOI:10.1152/jn.00523.2016


  8 / 1479 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27617735
[Au] Autor:Fox ME; Bucher ES; Johnson JA; Wightman RM
[Ad] Endereço:Department of Chemistry, Neuroscience Center, University of North Carolina at Chapel Hill , Chapel Hill, North Carolina 27599-3290, United States.
[Ti] Título:Medullary Norepinephrine Projections Release Norepinephrine into the Contralateral Bed Nucleus of the Stria Terminalis.
[So] Source:ACS Chem Neurosci;7(12):1681-1689, 2016 Dec 21.
[Is] ISSN:1948-7193
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Central norepinephrine signaling influences a wide range of behavioral and physiological processes, and the ventral bed nucleus of the stria terminalis (vBNST) receives some of the densest norepinephrine innervation in the brain. Previous work describes norepinephrine neurons as projecting primarily unilaterally; however, recent evidence for cross-hemispheric catecholamine signaling challenges this idea. Here, we use fast-scan cyclic voltammetry and retrograde tracing to characterize cross-hemispheric norepinephrine signaling in the vBNST. We delivered stimulations to noradrenergic pathways originating in the A1/A2 and locus coeruleus and found hemispherically equivalent norepinephrine release in the vBNST regardless of stimulated hemisphere. Unilateral retrograde tracing revealed that medullary, but not locus coeruleus norepinephrine neurons send cross-hemispheric projections to the vBNST. Further characterization with pharmacological lesions revealed that stimulations of the locus coeruleus and its axon bundles likely elicit vBNST norepinephrine release through indirect activation. These experiments are the first to demonstrate contralateral norepinephrine release and establish that medullary, but not coerulean neurons are responsible for norepinephrine release in the vBNST.
[Mh] Termos MeSH primário: Lateralidade Funcional
Bulbo/metabolismo
Neurônios/metabolismo
Norepinefrina/metabolismo
Núcleos Septais/metabolismo
[Mh] Termos MeSH secundário: Animais
Estimulação Elétrica
Lateralidade Funcional/fisiologia
Ácido Ibotênico
Locus Cerúleo/citologia
Locus Cerúleo/lesões
Locus Cerúleo/metabolismo
Masculino
Bulbo/citologia
Vias Neurais/citologia
Vias Neurais/metabolismo
Técnicas de Rastreamento Neuroanatômico
Marcadores do Trato Nervoso
Neurônios/citologia
Oxidopamina
Ratos Sprague-Dawley
Núcleos Septais/citologia
Estilbamidinas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (2-hydroxy-4,4'-diamidinostilbene, methanesulfonate salt); 0 (Neuronal Tract-Tracers); 0 (Stilbamidines); 2552-55-8 (Ibotenic Acid); 8HW4YBZ748 (Oxidopamine); X4W3ENH1CV (Norepinephrine)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171020
[Lr] Data última revisão:
171020
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160913
[St] Status:MEDLINE
[do] DOI:10.1021/acschemneuro.6b00210


  9 / 1479 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27616276
[Au] Autor:Matsumoto K; Takagi K; Kato A; Ishibashi T; Mori Y; Tashima K; Mitsumoto A; Kato S; Horie S
[Ad] Endereço:Laboratory of Pharmacology, Faculty of Pharmaceutical Sciences, Josai International University, Togane, Japan; Department of Pharmacology and Experimental Therapeutics, Kyoto Pharmaceutical University, Misasagi, Japan. Electronic address: kenjiro@mb.kyoto-phu.ac.jp.
[Ti] Título:Role of transient receptor potential melastatin 2 (TRPM2) channels in visceral nociception and hypersensitivity.
[So] Source:Exp Neurol;285(Pt A):41-50, 2016 Nov.
[Is] ISSN:1090-2430
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Transient receptor potential melastatin 2 (TRPM2) is a thermosensitive, Ca -permeable cation channel. TRPM2 contributes to the pathogenesis of inflammatory bowel disease, and inflammatory and neuropathic pain. We hypothesized that TRPM2 is important for visceral nociception and the development of visceral hypersensitivity. Therefore, we investigated the expression of TRPM2 channels and their involvement in visceral nociception in normal physiology and under pathological conditions that cause visceral hypersensitivity in rats. TRPM2 immunoreactivities were detected in the mucosa and muscle layer of the rat gastrointestinal tract. TRPM2 immunopositive cell bodies were almost completely co-localized with calretinin- and NeuN-positive cells in the myenteric plexus. We found that the majority of the TRPM2-immunoreactive cells were double-labeled with the retrograde marker fluorogold in lumbar 6/sacral 1 dorsal root ganglia (DRG), indicating that TRPM2 is expressed in spinal primary afferents innervating the distal colon. Subtypes of TRPM2-immunopositive DRG neurons were labeled by the A-fiber marker NF200, the C-fiber marker IB , substance P, calcitonin gene-related peptide, or P2X3 receptor. We found that oral administration of the TRPM2 inhibitor econazole (30mg/kg) reduced the visceromotor response (VMR) to noxious colorectal distention (CRD) at 80mmHg in control rats. Expression of TRPM2 in the mucosa of the distal colon was increased in a trinitrobenzene sulfonic acid-induced colitis model. The VMR to CRD significantly increased in colitis model rats compared with control rats at 40, 60, and 80mmHg. Econazole restored visceral hypersensitivity to the control level. Furthermore, TRPM2-deficient mice showed significantly attenuated trinitrobenzene sulfonic acid induced visceral hypersensitivity compared with wild-type mice. In conclusion, TRPM2 channels contribute to visceral nociception in response to noxious stimuli under normal conditions and visceral hypersensitivity in pathological conditions.
[Mh] Termos MeSH primário: Hipersensibilidade/metabolismo
Canais de Cátion TRPM/metabolismo
Dor Visceral/metabolismo
[Mh] Termos MeSH secundário: Animais
Antígenos CD/metabolismo
Calbindina 2/metabolismo
Proteínas de Ligação ao Cálcio
Colite/induzido quimicamente
Colite/complicações
Colo/inervação
Dextranos/farmacocinética
Modelos Animais de Doenças
Eletromiografia
Potencial Evocado Motor/efeitos dos fármacos
Potencial Evocado Motor/fisiologia
Fluoresceína-5-Isotiocianato/análogos & derivados
Fluoresceína-5-Isotiocianato/farmacocinética
Gânglios Espinais/citologia
Trato Gastrointestinal/inervação
Trato Gastrointestinal/metabolismo
Hipersensibilidade/genética
Cadeias alfa de Integrinas/metabolismo
Masculino
Camundongos
Camundongos Endogâmicos C57BL
Camundongos Transgênicos
Proteínas dos Microfilamentos
Proteínas do Tecido Nervoso/metabolismo
Ratos
Ratos Sprague-Dawley
Receptores Purinérgicos P2X/metabolismo
Estilbamidinas/farmacocinética
Substância P/metabolismo
Canais de Cátion TRPM/genética
Ácido Trinitrobenzenossulfônico/toxicidade
Dor Visceral/etiologia
Dor Visceral/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (2-hydroxy-4,4'-diamidinostilbene, methanesulfonate salt); 0 (Aif1 protein, mouse); 0 (Antigens, CD); 0 (Calbindin 2); 0 (Calcium-Binding Proteins); 0 (Dextrans); 0 (Integrin alpha Chains); 0 (Microfilament Proteins); 0 (Nerve Tissue Proteins); 0 (Receptors, Purinergic P2X); 0 (Stilbamidines); 0 (TRPM Cation Channels); 0 (TRPM2 protein, mouse); 0 (alpha E integrins); 0 (fluorescein isothiocyanate dextran); 33507-63-0 (Substance P); 8T3HQG2ZC4 (Trinitrobenzenesulfonic Acid); I223NX31W9 (Fluorescein-5-isothiocyanate)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170916
[Lr] Data última revisão:
170916
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160913
[St] Status:MEDLINE


  10 / 1479 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27436534
[Au] Autor:Ni RJ; Luo PH; Shu YM; Chen JT; Zhou JN
[Ad] Endereço:Chinese Academy of Science Key Laboratory of Brain Function and Diseases, School of Life Sciences, University of Science and Technology of China, Hefei 230027, Anhui, PR China.
[Ti] Título:Whole-brain mapping of afferent projections to the bed nucleus of the stria terminalis in tree shrews.
[So] Source:Neuroscience;333:162-80, 2016 Oct 01.
[Is] ISSN:1873-7544
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The bed nucleus of the stria terminalis (BST) plays an important role in integrating and relaying input information to other brain regions in response to stress. The cytoarchitecture of the BST in tree shrews (Tupaia belangeri chinensis) has been comprehensively described in our previous publications. However, the inputs to the BST have not been described in previous reports. The aim of the present study was to investigate the sources of afferent projections to the BST throughout the brain of tree shrews using the retrograde tracer Fluoro-Gold (FG). The present results provide the first detailed whole-brain mapping of BST-projecting neurons in the tree shrew brain. The BST was densely innervated by the prefrontal cortex, entorhinal cortex, ventral subiculum, amygdala, ventral tegmental area, and parabrachial nucleus. Moreover, moderate projections to the BST originated from the medial preoptic area, supramammillary nucleus, paraventricular thalamic nucleus, pedunculopontine tegmental nucleus, dorsal raphe nucleus, locus coeruleus, and nucleus of the solitary tract. Afferent projections to the BST are identified in the ventral pallidum, nucleus of the diagonal band, ventral posteromedial thalamic nucleus, posterior complex of the thalamus, interfascicular nucleus, retrorubral field, rhabdoid nucleus, intermediate reticular nucleus, and parvicellular reticular nucleus. In addition, the different densities of BST-projecting neurons in various regions were analyzed in the tree shrew brains. In summary, whole-brain mapping of direct inputs to the BST is delineated in tree shrews. These brain circuits are implicated in the regulation of numerous physiological and behavioral processes including stress, reward, food intake, and arousal.
[Mh] Termos MeSH primário: Núcleos Septais/anatomia & histologia
Tupaiidae/anatomia & histologia
[Mh] Termos MeSH secundário: Vias Aferentes/anatomia & histologia
Animais
Imuno-Histoquímica
Masculino
Técnicas de Rastreamento Neuroanatômico
Marcadores do Trato Nervoso
Fotomicrografia
Estilbamidinas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (2-hydroxy-4,4'-diamidinostilbene, methanesulfonate salt); 0 (Neuronal Tract-Tracers); 0 (Stilbamidines)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160721
[St] Status:MEDLINE



página 1 de 148 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde