Base de dados : MEDLINE
Pesquisa : D02.092.146.215 [Categoria DeCS]
Referências encontradas : 158 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 16 ir para página                         

  1 / 158 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28411775
[Au] Autor:Elston DM; Liranzo MO; Scollard DM
[Ad] Endereço:Department of Dermatology and Dermatologic Surgery, The Medical University of South Carolina, Charleston, South Carolina. Electronic address: elstond@musc.edu.
[Ti] Título:Comparing the sensitivity of auramine-rhodamine fluorescence to polymerase chain reaction in the detection of Mycobacterium leprae in Fite-negative tissue sections.
[So] Source:J Am Acad Dermatol;76(5):992-993, 2017 05.
[Is] ISSN:1097-6787
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Benzofenoneídio
Mycobacterium leprae/isolamento & purificação
Reação em Cadeia da Polimerase
Rodaminas
[Mh] Termos MeSH secundário: Fluorescência
Seres Humanos
[Pt] Tipo de publicação:COMPARATIVE STUDY; LETTER
[Nm] Nome de substância:
0 (Rhodamines); 2465-27-2 (Benzophenoneidum)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171002
[Lr] Data última revisão:
171002
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170417
[St] Status:MEDLINE


  2 / 158 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28208061
[Au] Autor:Murata M; Uchida Y; Takami T; Ito T; Anzai R; Sonotaki S; Murakami Y
[Ad] Endereço:Department of Organic and Polymer Materials Chemistry, Faculty of Engineering, Tokyo University of Agriculture and Technology, Japan.
[Ti] Título:Dual drug release from hydrogels covalently containing polymeric micelles that possess different drug release properties.
[So] Source:Colloids Surf B Biointerfaces;153:19-26, 2017 May 01.
[Is] ISSN:1873-4367
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:In the present study, we designed hydrogels for dual drug release: the hydrogels that covalently contained the polymeric micelles that possess different drug release properties. The hydrogels that were formed from polymeric micelles possessing a tightly packed (i.e., well-entangled) inner core exhibited a higher storage modulus than the hydrogels that were formed from the polymeric micelles possessing a loosely packed structure. Furthermore, we conducted release experiments and fluorescent observations to evaluate the profiles depicting the release of two compounds, rhodamine B and auramine O, from either polymeric micelles or hydrogels. According to our results, (1) hydrogels that covalently contains polymeric micelles that possess different drug release properties successfully exhibit the independent release behaviors of the two compounds and (2) fluorescence microscopy can greatly facilitate efforts to evaluate drug release properties of materials.
[Mh] Termos MeSH primário: Benzofenoneídio/química
Liberação Controlada de Fármacos
Hidrogéis/química
Micelas
Poliésteres/química
Polietilenoglicóis/química
Rodaminas/química
[Mh] Termos MeSH secundário: Hidrogéis/síntese química
Microscopia de Fluorescência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Hydrogels); 0 (Micelles); 0 (Polyesters); 0 (Rhodamines); 2465-27-2 (Benzophenoneidum); 30IQX730WE (Polyethylene Glycols); 459TN2L5F5 (poly(lactide)); K7G5SCF8IL (rhodamine B)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170417
[Lr] Data última revisão:
170417
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170217
[St] Status:MEDLINE


  3 / 158 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28049087
[Au] Autor:Sukriti; Sharma J; Chadha AS; Pruthi V; Anand P; Bhatia J; Kaith BS
[Ad] Endereço:Research and Development Laboratory, Department of Chemistry, Dr B.R. Ambedkar National Institute of Technology, Jalandhar, Punjab, India. Electronic address: sukriti.chemistry@gmail.com.
[Ti] Título:Sequestration of dyes from artificially prepared textile effluent using RSM-CCD optimized hybrid backbone based adsorbent-kinetic and equilibrium studies.
[So] Source:J Environ Manage;190:176-187, 2017 Apr 01.
[Is] ISSN:1095-8630
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Present work reports the synthesis of semi-Interpenetrating Network Polymer (semi-IPN) using Gelatin-Gum xanthan hybrid backbone and polyvinyl alcohol in presence of l-tartaric acid and ammonium persulphate as the crosslinker-initiator system. Reaction parameters were optimized with Response Surface Methodology (RSM) in order to maximize the percent gel fraction of the synthesized sample. Polyvinyl alcohol, l-Tartaric acid, ammonium persulphate, reaction temperature, time and pH of the reaction medium were found to make an impact on the percentage gel fraction obtained. Incorporation of polyvinyl alcohol chains onto hybrid backbone and crosslinking between the different polymer chains were confirmed through techniques like FTIR, SEM-EDX and XRD. Semi-IPN was found to be very efficient in the removal of cationic dyes rhodamine-B (70%) and auramine-O (63%) from a mixture with an adsorbent dose of 700 mg, initial concentration of rhodamine-B 6 mgL and auramine-O 26 mgL , at an time interval of 22-25 h and 30 °C temp. Further to determine the nature of adsorption Langmuir and Freundlich adsorption isotherm models were studied and it was found that Langmuir adsorption isotherm was the best fit model for the removal of mixture of dyes. Kinetic studies for the sorption of dyes favored the reaction mechanism to occur via a pseudo second order pathway with R value about 0.99.
[Mh] Termos MeSH primário: Corantes/isolamento & purificação
Polímeros/química
Indústria Têxtil
Eliminação de Resíduos Líquidos/métodos
[Mh] Termos MeSH secundário: Adsorção
Benzofenoneídio/química
Benzofenoneídio/isolamento & purificação
Corantes/química
Gelatina/química
Concentração de Íons de Hidrogênio
Cinética
Polímeros/síntese química
Polissacarídeos Bacterianos/química
Álcool de Polivinil/química
Rodaminas/química
Rodaminas/isolamento & purificação
Espectroscopia de Infravermelho com Transformada de Fourier
Tartaratos/química
Temperatura Ambiente
Poluentes Químicos da Água/química
Poluentes Químicos da Água/isolamento & purificação
Difração de Raios X
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Coloring Agents); 0 (Polymers); 0 (Polysaccharides, Bacterial); 0 (Rhodamines); 0 (Tartrates); 0 (Water Pollutants, Chemical); 2465-27-2 (Benzophenoneidum); 9000-70-8 (Gelatin); 9002-89-5 (Polyvinyl Alcohol); K7G5SCF8IL (rhodamine B); TTV12P4NEE (xanthan gum); W4888I119H (tartaric acid)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170104
[St] Status:MEDLINE


  4 / 158 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27291283
[Au] Autor:Allassia S; Aranibar M; Boutonnet M; Caserío V; Etchart AA; Fajardo S; García M; Gomez N; Gunia AM; Gustincic MV; Izquierdo V; Jara AA; Kozicky G; Matteo M; Pellegrini C; Pellegrino S; Pérez Catalán S; Poggi S; Sacramone C; Santiso GM; Souto A; Togneri AM; Wolff L; Vilche S; Eletti D; Imaz MS
[Ad] Endereço:Dirección de Bioquímica de la Municipalidad de Rosario, Rosario, Santa Fe, Argentina.
[Ti] Título:LED fluorescence microscopy in the diagnosis of tuberculosis: Fading and restaining of smears for external quality assessment.
[So] Source:Rev Argent Microbiol;48(2):122-7, 2016 Apr-Jun.
[Is] ISSN:0325-7541
[Cp] País de publicação:Argentina
[La] Idioma:eng
[Ab] Resumo:Blinded rechecking is a method proposed for external quality assurance (EQA) of auramine-stained acid-fast bacilli (AFB) smears using fluorescence microscopy (FM), however, this procedure is not well developed and slides fading over time could compromise its implementation. Since bleaching of fluorescent molecules involves temperature-dependent chemical reactions, it is likely that low temperatures could slow down this process. We stored auramine-stained slides under different environmental conditions, including -20°C, and examined them over time. The slides stored in all the environments faded. At -20°C, fading was not reduced in relation to room temperature. Restaining and re-examining smears after five months showed that the slides containing saliva and storage at -20°C were associated with failure in AFB reappearance. In conclusion, the practice of freezing slides until they are viewed should be discouraged as it has a negative effect on blinded rechecking by reducing reading concordance after restaining. Specimen quality should be considered when interpreting FM-EQA results.
[Mh] Termos MeSH primário: Benzofenoneídio/efeitos da radiação
Corantes Fluorescentes/efeitos da radiação
Microscopia de Fluorescência/métodos
Fotodegradação
Garantia da Qualidade dos Cuidados de Saúde/métodos
Escarro/microbiologia
Coloração e Rotulagem/métodos
Tuberculose/diagnóstico
[Mh] Termos MeSH secundário: Argentina
Benzofenoneídio/análise
Criopreservação
Estudos de Viabilidade
Corantes Fluorescentes/análise
Seres Humanos
Iluminação
Microscopia de Fluorescência/instrumentação
Variações Dependentes do Observador
Valor Preditivo dos Testes
Preservação Biológica/métodos
Reprodutibilidade dos Testes
Método Simples-Cego
Temperatura Ambiente
[Pt] Tipo de publicação:COMPARATIVE STUDY; EVALUATION STUDIES; JOURNAL ARTICLE; MULTICENTER STUDY
[Nm] Nome de substância:
0 (Fluorescent Dyes); 2465-27-2 (Benzophenoneidum)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170403
[Lr] Data última revisão:
170403
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160614
[St] Status:MEDLINE


  5 / 158 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:26318558
[Au] Autor:Kayigire XA; Friedrich SO; van der Merwe L; Donald PR; Diacon AH
[Ad] Endereço:Division of Medical Physiology, MRC Centre for Tuberculosis Research, DST/NRF Centre of Excellence for Biomedical Tuberculosis Research, Faculty of Medicine and Health Sciences, Stellenbosch University, Tygerberg, South Africa; Task Applied Science, Bellville, Cape Town, South Africa.
[Ti] Título:Simultaneous staining of sputum smears for acid-fast and lipid-containing Myobacterium tuberculosis can enhance the clinical evaluation of antituberculosis treatments.
[So] Source:Tuberculosis (Edinb);95(6):770-779, 2015 Dec.
[Is] ISSN:1873-281X
[Cp] País de publicação:Scotland
[La] Idioma:eng
[Ab] Resumo:Dormant, slow-growing, antibiotic-tolerant Mycobacterium tuberculosis undermine the shortening of tuberculosis treatment to less than 6 months and are thought to be characterised by intracellular lipid bodies. Antibiotic effects on such persisting bacilli escape evaluation as they cannot be readily cultured. We identified cells containing lipid bodies in sputum smears from 86 newly diagnosed pulmonary tuberculosis patients and monitored these cells daily in 42 patients over the first 14 days of treatment with rifampicin, the experimental compound SQ-109, or both agents combined. Counts of Nile-Red-positive lipid-body containing cells were correlated with those of Auramine-O-positive cells and colony forming units of viable Mycobacterium tuberculosis on agar plates. Rifampicin but not SQ-109 significantly reduced colony forming units but all treatments distinctively and significantly changed the proportions of lipid body-containing bacilli and viable Mycobacterium tuberculosis. Monitoring lipid-body containing bacilli in sputum during treatment with experimental antituberculosis regimens may identify putative treatment-shortening regimens.
[Mh] Termos MeSH primário: Adamantano/análogos & derivados
Antituberculosos/uso terapêutico
Monitoramento de Medicamentos/métodos
Etilenodiaminas/uso terapêutico
Gotículas Lipídicas/metabolismo
Microscopia de Fluorescência
Mycobacterium tuberculosis/efeitos dos fármacos
Rifampina/uso terapêutico
Tuberculose Pulmonar/tratamento farmacológico
[Mh] Termos MeSH secundário: Adamantano/uso terapêutico
Carga Bacteriana
Benzofenoneídio
Contagem de Colônia Microbiana
Quimioterapia Combinada
Corantes Fluorescentes
Seres Humanos
Mycobacterium tuberculosis/crescimento & desenvolvimento
Mycobacterium tuberculosis/metabolismo
Oxazinas
Valor Preditivo dos Testes
África do Sul
Escarro/microbiologia
Fatores de Tempo
Resultado do Tratamento
Tuberculose Pulmonar/diagnóstico
Tuberculose Pulmonar/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; MULTICENTER STUDY; RANDOMIZED CONTROLLED TRIAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antitubercular Agents); 0 (Ethylenediamines); 0 (Fluorescent Dyes); 0 (N-geranyl-N'-(2-adamantyl)ethane-1,2-diamine); 0 (Oxazines); 2465-27-2 (Benzophenoneidum); P476F1L81G (nile red); PJY633525U (Adamantane); VJT6J7R4TR (Rifampin)
[Em] Mês de entrada:1609
[Cu] Atualização por classe:170917
[Lr] Data última revisão:
170917
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150831
[St] Status:MEDLINE


  6 / 158 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:25898317
[Au] Autor:Xu H; Geng F; Wang Y; Xu M; Lai X; Qu P; Zhang Y; Liu B
[Ad] Endereço:Department of Chemistry, State Key Lab of Molecular Engineering of Polymers and Institutes of Biomedical Sciences, Fudan University, Shanghai 200433, China. bhliu@fudan.edu.cn.
[Ti] Título:A label-free fluorescent molecular switch for a DNA hybridization assay utilizing a G-quadruplex-selective auramine O.
[So] Source:Chem Commun (Camb);51(41):8622-5, 2015 May 21.
[Is] ISSN:1364-548X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A sensitive and selective assay of DNA is developed by utilizing a signal transduction strategy with the rational redesign of the hairpin structured G-quadruplex molecular switch (G4-MS) assembled using auramine O (AO). By monitoring the changes of the fluorescent signal, we could identify and further quantitatively determine the target DNA in the samples.
[Mh] Termos MeSH primário: Benzofenoneídio/química
DNA/análise
Corantes Fluorescentes/química
Quadruplex G
Hibridização de Ácido Nucleico
[Mh] Termos MeSH secundário: Seres Humanos
Estrutura Molecular
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Fluorescent Dyes); 2465-27-2 (Benzophenoneidum); 9007-49-2 (DNA)
[Em] Mês de entrada:1602
[Cu] Atualização por classe:150507
[Lr] Data última revisão:
150507
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150422
[St] Status:MEDLINE
[do] DOI:10.1039/c5cc02624e


  7 / 158 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:25782178
[Au] Autor:Asfaram A; Ghaedi M; Hajati S; Goudarzi A; Bazrafshan AA
[Ad] Endereço:Chemistry Department, Yasouj University, Yasouj 75918-74831, Iran.
[Ti] Título:Simultaneous ultrasound-assisted ternary adsorption of dyes onto copper-doped zinc sulfide nanoparticles loaded on activated carbon: optimization by response surface methodology.
[So] Source:Spectrochim Acta A Mol Biomol Spectrosc;145:203-12, 2015 Jun 15.
[Is] ISSN:1873-3557
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The simultaneous and competitive ultrasound-assisted removal of Auramine-O (AO), Erythrosine (Er) and Methylene Blue (MB) from aqueous solutions were rapidly performed onto copper-doped zinc sulfide nanoparticles loaded on activated carbon (ZnS:Cu-NP-AC). ZnS:Cu nanoparticles were studied by FESEM, XRD and TEM. First, the effect of pH was optimized in a one-at-a-time procedure. Then the dependency of dyes removal percentage in their ternary solution on the level and magnitude of variables such as sonication time, initial dyes concentrations and adsorbent dosage was fully investigated and optimized by central composite design (CCD) under response surface methodology (RSM) as well as by regarding desirability function (DF) as a good and general criterion. The good agreement found between experimental and predicted values supports and confirms the suitability of the present model to predict adsorption state. The applied ultrasound strongly enhanced mass transfer process and subsequently performance. Hence, a small amount of the adsorbent (0.04 g) was capable to remove high percentage of dyes, i.e. 100%, 99.6% and 100% for MB, AO and Er, respectively, in very short time (2.5 min). The experimental equilibrium data fitting to Langmuir, Freundlich, Temkin and Dubinin-Radushkevich models showed that the Langmuir model applies well for the evaluation and description of the actual behavior of adsorption. The small amount of proposed adsorbent (0.015 g) was applicable for successful removal of dyes (RE>99.0%) in short time (2.5 min) with high adsorption capacity in single component system (123.5 mg g(-1) for MB, 123 mg g(-1) for AO and 84.5 mg g(-1) for Er). Kinetics evaluation of experiments at various time intervals reveals that adsorption processes can be well predicated and fitted by pseudo-second-order and Elovich models.
[Mh] Termos MeSH primário: Carvão Vegetal/química
Corantes/química
Cobre/química
Nanopartículas/química
Sulfetos/química
Ultrassom/métodos
Compostos de Zinco/química
[Mh] Termos MeSH secundário: Adsorção
Análise de Variância
Benzofenoneídio/química
Eritrosina/química
Concentração de Íons de Hidrogênio
Cinética
Azul de Metileno/química
Nanopartículas/ultraestrutura
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Coloring Agents); 0 (Sulfides); 0 (Zinc Compounds); 16291-96-6 (Charcoal); 2465-27-2 (Benzophenoneidum); 789U1901C5 (Copper); KPS085631O (zinc sulfide); PN2ZH5LOQY (Erythrosine); T42P99266K (Methylene Blue)
[Em] Mês de entrada:1601
[Cu] Atualização por classe:150410
[Lr] Data última revisão:
150410
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150318
[St] Status:MEDLINE


  8 / 158 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:25578262
[Au] Autor:Rueda ZV; López L; Marín D; Vélez LA; Arbeláez MP
[Ad] Endereço:Grupo Investigador de Problemas en Enfermedades Infecciosas, Facultad de Medicina, Calle 62 # 52-59, lab 630, Sede de Investigación Universitaria, Universidad de Antioquia, Medellín, Colombia. Electronic address: zulmaruedav@gmail.com.
[Ti] Título:Sputum induction is a safe procedure to use in prisoners and MGIT is the best culture method to diagnose tuberculosis in prisons: a cohort study.
[So] Source:Int J Infect Dis;33:82-8, 2015 Apr.
[Is] ISSN:1878-3511
[Cp] País de publicação:Canada
[La] Idioma:eng
[Ab] Resumo:OBJECTIVES: To evaluate the concordance and safety of induced sputum (IS) and spontaneous sputum (SS), and estimate concordance and time to detection of M. tuberculosis between Lowenstein-Jensen (LJ), thin-layer agar (TLA), and the Mycobacteria Growth Indicator Tube system (MGIT). METHODS: This was a cohort study. Prisoners with pulmonary tuberculosis (PTB) were followed for 2 years. At baseline and every follow-up visit, three sputum samples were taken on consecutive days (one IS and two SS) and adverse events occurring before, during, and 30 min after IS were registered. All sputum samples were stained with auramine and cultured in LJ, TLA (to test resistance), and MGIT. RESULTS: Five hundred eighty-six IS and 532 SS were performed on 64 PTB patients. Breathlessness (1.6%), cough (1.2%), hemoptysis (0.3%), and cyanosis (0.2%) were the only complications. Concordance between IS and SS was 0.78 (95% confidence interval 0.69-0.87); 11 positive cultures from IS samples were negative in SS, and 11 positive cultures from SS samples were negative in IS. One hundred seventy-eight cultures were positive by any technique: MGIT 95%, LJ 73%, and TLA 57%. Time to detection of M. tuberculosis in LJ, TLA, and MGIT was 31, 18, and 11 days, respectively. CONCLUSIONS: The IS procedure is safe in prisons. The MGIT system is better and faster than LJ and TLA in the diagnosis of M. tuberculosis.
[Mh] Termos MeSH primário: Mycobacterium tuberculosis/isolamento & purificação
Prisioneiros
Escarro/microbiologia
Tuberculose Pulmonar/diagnóstico
[Mh] Termos MeSH secundário: Ágar
Técnicas Bacteriológicas
Benzofenoneídio
Estudos de Coortes
Corantes
Meios de Cultura
Técnicas de Diagnóstico do Sistema Respiratório/efeitos adversos
Feminino
Seres Humanos
Masculino
Estudos Prospectivos
Fatores de Tempo
[Pt] Tipo de publicação:CLINICAL TRIAL; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Coloring Agents); 0 (Culture Media); 2465-27-2 (Benzophenoneidum); 9002-18-0 (Agar)
[Em] Mês de entrada:1602
[Cu] Atualização por classe:150413
[Lr] Data última revisão:
150413
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150113
[St] Status:MEDLINE


  9 / 158 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:25459504
[Au] Autor:Ghaedi M; Khodadoust S; Sadeghi H; Khodadoust MA; Armand R; Fatehi A
[Ad] Endereço:Chemistry Department, Yasouj University, Yasouj, Iran.
[Ti] Título:Application of ultrasonic radiation for simultaneous removal of auramine O and safranine O by copper sulfide nanoparticles: experimental design.
[So] Source:Spectrochim Acta A Mol Biomol Spectrosc;136 Pt B:1069-75, 2015 Feb 05.
[Is] ISSN:1873-3557
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In this study, copper sulfide nanoparticles loaded on activated carbon (CuS-NP-AC) were synthesized by novel, low cost and green approach and characterized using SEM and XRD. The application of this material for the simulations removal of auramine O (AO) and safranine O (SO) from aqueous solutions was investigated. The dependency of removal percentages to variables such as pH, initial dyes concentration, adsorbent dosage, sonication time and sonication temperature were studied with response surface methodology (RSM) by considering the desirability function (DF). The quadratic model between the dependent and the independent variables was built. The proposed method showed good agreement between the experimental data and predictive value, and it has been successfully employed to removal of AO and SO in aqueous media. The studied adsorbent (0.06 g of CuS-NP-AC) was capable of high percentage removal (99.8% and 99.5%) of 18 mg mL(-1) AO and SO in short time (7.0 min).
[Mh] Termos MeSH primário: Benzofenoneídio/isolamento & purificação
Cobre/química
Nanopartículas/química
Fenazinas/isolamento & purificação
Ultrassom
[Mh] Termos MeSH secundário: Adsorção
Análise de Variância
Nanopartículas/ultraestrutura
Difração de Raios X
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Phenazines); 2465-27-2 (Benzophenoneidum); 789U1901C5 (Copper); KL4YU612X7 (cupric sulfide); XTX0YXU2HV (safranine T)
[Em] Mês de entrada:1508
[Cu] Atualização por classe:151119
[Lr] Data última revisão:
151119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141203
[St] Status:MEDLINE


  10 / 158 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:25199013
[Au] Autor:Van Deun A; Aung KJ; Khan MH; de Jong BC; Gumusboga M; Hossain MA
[Ad] Endereço:International Union Against Tuberculosis and Lung Disease, Paris, France.
[Ti] Título:An operational study comparing microscopes and staining variations for tuberculosis LED FM.
[So] Source:Int J Tuberc Lung Dis;18(8):964-71, 2014 Aug.
[Is] ISSN:1815-7920
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:SETTING: Tuberculosis control projects, Damien Foundation Bangladesh. OBJECTIVES: To compare transmitted fluorescence (Olympus CX21™/FRAEN FluoLED™) with epi-fluorescence (Zeiss Primostar iLED™) light-emitting diode microscopes (LED-FM) and various auramine staining and destaining/counterstaining techniques for the detection of acid-fast bacilli. DESIGN: Multicentre blinded reading of routine smears on both types of microscopes using different staining techniques in multiple phases. LED-FM rechecking of discordant series with and without restaining to calculate operating characteristics. RESULTS: Among 64 874 smears, both instruments detected 9.6% positives. Compared to the standard technique, the stronger auramine-O formulation did not perform better. Thiazine red counterstaining tended to yield more false-positive as well as false-negative errors. Combined destaining/counterstaining (sensitivity 93%, positive predictive value [PPV] 98%) proved significantly less effective. Both destaining with 1% hydrochloric acid (HCl) and 10% alcohol and the standard 0.5% HCl and 70-95% alcohol were equally accurate (sensitivity 95-96%, PPV 99%). The sturdiness of the microscopes in field conditions was sub-optimal: only 5/16 instruments did not break down. CONCLUSIONS: Both microscopes performed equally well. The standard staining technique is as good as the more complicated and expensive variations. A destaining solution containing only 10% alcohol works perfectly well. The inferior quality of LED-FM microscope components is an obstacle to FM expansion.
[Mh] Termos MeSH primário: Microscopia de Fluorescência/métodos
Escarro/microbiologia
Tuberculose/diagnóstico
[Mh] Termos MeSH secundário: Benzofenoneídio/química
Reações Falso-Negativas
Reações Falso-Positivas
Compostos Orgânicos/química
Valor Preditivo dos Testes
Reprodutibilidade dos Testes
Sensibilidade e Especificidade
Coloração e Rotulagem
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; MULTICENTER STUDY; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Organic Chemicals); 0 (thiazin red); 2465-27-2 (Benzophenoneidum)
[Em] Mês de entrada:1505
[Cu] Atualização por classe:140909
[Lr] Data última revisão:
140909
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140909
[St] Status:MEDLINE
[do] DOI:10.5588/ijtld.14.0077



página 1 de 16 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde