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[PMID]: | 27619487 |
[Au] Autor: | Boglino A; Ponce M; Cousin X; Gisbert E; Manchado M |
[Ad] Endereço: | Centro de Investigación en Alimentación y Desarrollo, Unidad Mazatlán (CIAD), PO Box 711, 82010 Mazatlán, Sinaloa, Mexico. |
[Ti] Título: | Transcriptional regulation of genes involved in retinoic acid metabolism in Senegalese sole larvae. |
[So] Source: | Comp Biochem Physiol B Biochem Mol Biol;203:35-46, 2017 Jan. | [Is] ISSN: | 1879-1107 |
[Cp] País de publicação: | England |
[La] Idioma: | eng |
[Ab] Resumo: | The aim of this study was the characterization of transcriptional regulatory pathways mediated by retinoic acid (RA) in Senegalese sole larvae. For this purpose, pre-metamorphic larvae were treated with a low concentration of DEAB, an inhibitor of RALDH enzyme, until the end of metamorphosis. No differences in growth, eye migration or survival were observed. Nevertheless, gene expression analysis revealed a total of 20 transcripts differentially expressed during larval development and only six related with DEAB treatments directly involved in RA metabolism and actions (rdh10a, aldh1a2, crbp1, igf2r, rarg and cyp26a1) to adapt to a low-RA environment. In a second experiment, post-metamorphic larvae were exposed to the all-trans RA (atRA) observing an opposite regulation for those genes involved in RA synthesis and degradation (rdh10a, aldh1a2, crbp1 and cyp26a1) as well as other related with thyroid- (dio2) and IGF-axes (igfbp1, igf2r and igfbp5) to balance RA levels. In a third experiment, DEAB-pretreated post-metamorphic larvae were exposed to atRA and TTNPB (a specific RAR agonist). Both drugs down-regulated rdh10a and aldh1a2 and up-regulated cyp26a1 expression demonstrating their important role in RA homeostasis. Moreover, five retinoic receptors that mediate RA actions, the thyroid receptor thrb, and five IGF binding proteins changed differentially their expression. Overall, this study demonstrates that exogenous RA modulates the expression of some genes involved in the RA synthesis, degradation and cellular transport through RAR-mediated regulatory pathways establishing a negative feedback regulatory mechanism necessary to balance endogenous RA levels and gradients. |
[Mh] Termos MeSH primário: |
Linguados/genética Linguados/metabolismo Regulação da Expressão Gênica Larva/genética Larva/metabolismo Tretinoína/metabolismo
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[Mh] Termos MeSH secundário: |
Animais Benzoatos/farmacologia Regulação da Expressão Gênica/efeitos dos fármacos Larva/crescimento & desenvolvimento Metamorfose Biológica/efeitos dos fármacos Metamorfose Biológica/genética Receptores do Ácido Retinoico/agonistas Receptores do Ácido Retinoico/genética Receptores do Ácido Retinoico/metabolismo Retinoides/farmacologia p-Aminoazobenzeno/análogos & derivados p-Aminoazobenzeno/farmacologia
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[Pt] Tipo de publicação: | JOURNAL ARTICLE |
[Nm] Nome de substância:
| 0 (Benzoates); 0 (Receptors, Retinoic Acid); 0 (Retinoids); 2481-94-9 (C.I. Solvent Yellow 56); 5688UTC01R (Tretinoin); 57X2AH42T1 (p-Aminoazobenzene); 71441-28-6 (4-(2-(5,6,7,8-tetrahydro-5,5,8,8-tetramethyl-2-naphthalenyl)-1-propenyl)benzoic acid) |
[Em] Mês de entrada: | 1702 |
[Cu] Atualização por classe: | 170206 |
[Lr] Data última revisão:
| 170206 |
[Sb] Subgrupo de revista: | IM |
[Da] Data de entrada para processamento: | 161106 |
[St] Status: | MEDLINE |
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