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[PMID]:28945359
[Au] Autor:Mahmoud MM; Schechter A; Alnajjar KS; Huang J; Towle-Weicksel J; Eckenroth BE; Doublié S; Sweasy JB
[Ad] Endereço:Department of Therapeutic Radiology, Yale University School of Medicine , New Haven, Connecticut 06520, United States.
[Ti] Título:Defective Nucleotide Release by DNA Polymerase ß Mutator Variant E288K Is the Basis of Its Low Fidelity.
[So] Source:Biochemistry;56(41):5550-5559, 2017 Oct 17.
[Is] ISSN:1520-4995
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:DNA polymerases synthesize new DNA during DNA replication and repair, and their ability to do so faithfully is essential to maintaining genomic integrity. DNA polymerase ß (Pol ß) functions in base excision repair to fill in single-nucleotide gaps, and variants of Pol ß have been associated with cancer. Specifically, the E288K Pol ß variant has been found in colon tumors and has been shown to display sequence-specific mutator activity. To probe the mechanism that may underlie E288K's loss of fidelity, a fluorescence resonance energy transfer system that utilizes a fluorophore on the fingers domain of Pol ß and a quencher on the DNA substrate was employed. Our results show that E288K utilizes an overall mechanism similar to that of wild type (WT) Pol ß when incorporating correct dNTP. However, when inserting the correct dNTP, E288K exhibits a faster rate of closing of the fingers domain combined with a slower rate of nucleotide release compared to those of WT Pol ß. We also detect enzyme closure upon mixing with the incorrect dNTP for E288K but not WT Pol ß. Taken together, our results suggest that E288K Pol ß incorporates all dNTPs more readily than WT because of an inherent defect that results in rapid isomerization of dNTPs within its active site. Structural modeling implies that this inherent defect is due to interaction of E288K with DNA, resulting in a stable closed enzyme structure.
[Mh] Termos MeSH primário: Neoplasias do Colo/enzimologia
DNA Polimerase beta/metabolismo
Reparo do DNA
Replicação do DNA
DNA/metabolismo
Modelos Moleculares
Mutação
[Mh] Termos MeSH secundário: Substituição de Aminoácidos
Biocatálise
Neoplasias do Colo/genética
DNA/química
DNA Polimerase beta/química
DNA Polimerase beta/genética
Estabilidade Enzimática
Corantes Fluorescentes/química
Seres Humanos
Cinética
Mutagênese Sítio-Dirigida
Naftalenossulfonatos/química
Proteínas de Neoplasias/química
Proteínas de Neoplasias/genética
Proteínas de Neoplasias/metabolismo
Conformação Proteica
Domínios e Motivos de Interação entre Proteínas
Redobramento de Proteína
Proteínas Recombinantes/química
Proteínas Recombinantes/metabolismo
Especificidade por Substrato
p-Dimetilaminoazobenzeno/análogos & derivados
p-Dimetilaminoazobenzeno/química
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fluorescent Dyes); 0 (Naphthalenesulfonates); 0 (Neoplasm Proteins); 0 (Recombinant Proteins); 6268-49-1 (4-(4-dimethylaminophenylazo)benzoic acid); 9007-49-2 (DNA); A49L8E13FD (p-Dimethylaminoazobenzene); EC 2.7.7.- (DNA Polymerase beta)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171026
[Lr] Data última revisão:
171026
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170926
[St] Status:MEDLINE
[do] DOI:10.1021/acs.biochem.7b00869


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[PMID]:28430776
[Au] Autor:Lin LY; Peng CC; Chen Y; Huang BC; Chang CC; Peng RY
[Ad] Endereço:Department of Food And Applied Technology, Hungkuang University, Shalu County, Taichung City,Taiwan.
[Ti] Título:Aminoazo dye-protein-adduct enhances inhibitory effect on digestibility and damages to Gastro-Duodenal-Hepatic axis.
[So] Source:PLoS One;12(4):e0170555, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:4-Dimethylaminoazobenzene (DAB, methyl yellow, or butter yellow), a human carcinogen, has been banned for use in foods since 1988. In 2014, DAB adulteration in Tofu occurred in Taiwan. We hypothesize that DAB can form [DAB•SBP]adduct adduct with soybean protein (SBP) which could damage Gastro-Duodenal-Hepatic axis. Sprague-Dawley rats gavage fed [DAB•SBP]adduct adduct revealed severely reduced body weight and damaged duodenum, liver, hepatic mitochondria, and spleen. Hepatic levels of glutathione and ATP were severely reduced. Serum GOT and GPT were substantially elevated. Analysis by the adsorption isotherm clearly revealed DAB formed very stable [DAB•SBP]adduct adduct at 1:1 molar ration (Phase A). The equilibrium constant of this colloidal adduct [DAB•SBP]adduct was KeqA = ∝, behaving as the most stable and toxic species. At higher protein concentration (Phase C) it formed conjugate [DAB×SBPgross]conjugate, with KeqC = 3.23×10-2 mg/mL, implicating a moderately strong adsorption. The in vitro pepsin digestibility test showed apparently reduced digestibility by 27% (by Ninhydrin assay) or 8% (by Bradford assay). Conclusively, this is the first report indicating that [DAB•SBP]adduct potentially is capable to damage the Gastro-Duodenal-Hepatic axis.
[Mh] Termos MeSH primário: Digestão/efeitos dos fármacos
Duodeno/efeitos dos fármacos
Fígado/efeitos dos fármacos
Estômago/efeitos dos fármacos
p-Dimetilaminoazobenzeno/farmacologia
[Mh] Termos MeSH secundário: Trifosfato de Adenosina/metabolismo
Animais
Peso Corporal/efeitos dos fármacos
Coloides
Duodeno/metabolismo
Duodeno/fisiologia
Glutationa/metabolismo
Fígado/metabolismo
Fígado/fisiologia
Masculino
Tamanho do Órgão/efeitos dos fármacos
Ratos
Ratos Sprague-Dawley
Estômago/metabolismo
Estômago/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Colloids); 8L70Q75FXE (Adenosine Triphosphate); A49L8E13FD (p-Dimethylaminoazobenzene); GAN16C9B8O (Glutathione)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170907
[Lr] Data última revisão:
170907
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170422
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0170555


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[PMID]:28063486
[Au] Autor:Cho W; Hu Y; Baek K; Kim H
[Ad] Endereço:University of Illinois at Chicago, Chicago, IL, United States; Kyung Hee University, Yongin, South Korea. Electronic address: wcho@uic.edu.
[Ti] Título:A High-Throughput Fluorometric Assay for Lipid-Protein Binding.
[So] Source:Methods Enzymol;583:1-18, 2017.
[Is] ISSN:1557-7988
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:An increasing number of intracellular and extracellular proteins are shown to interact with membrane lipids under physiological conditions. For rapid and robust quantitative measurement of lipid-protein interaction, we developed a sensitive fluorescence quenching-based assay that is universally applicable to all proteins and lipids. The assay employs fluorescence protein (FP)-tagged proteins whose fluorescence emission intensity is decreased when they bind vesicles containing quenching lipids. This simple assay can be performed with a fluorescence plate reader or a spectrofluorometer and optimized for different proteins with various combinations of FPs and quenching lipids. The assay allows a rapid, sensitive, and accurate determination of lipid specificity and affinity for various lipid-binding proteins, and high-throughput screening of molecules that modulate their membrane binding.
[Mh] Termos MeSH primário: Proteínas de Transporte/metabolismo
Ensaios de Triagem em Larga Escala
Lipídeos de Membrana/metabolismo
Fosfatos de Fosfatidilinositol/metabolismo
Proteínas Recombinantes de Fusão/metabolismo
Espectrometria de Fluorescência/métodos
[Mh] Termos MeSH secundário: Proteínas de Transporte/genética
Membrana Celular/química
Membrana Celular/metabolismo
Clonagem Molecular
Escherichia coli/genética
Escherichia coli/metabolismo
Corantes Fluorescentes/química
Expressão Gênica
Glutationa Transferase/genética
Glutationa Transferase/metabolismo
Proteínas de Fluorescência Verde/genética
Proteínas de Fluorescência Verde/metabolismo
Lipídeos de Membrana/química
Fosfatidilcolinas/química
Fosfatidilcolinas/metabolismo
Fosfatos de Fosfatidilinositol/química
Ligação Proteica
Proteínas Proto-Oncogênicas c-akt/genética
Proteínas Proto-Oncogênicas c-akt/metabolismo
Proteínas Recombinantes de Fusão/genética
Lipossomas Unilamelares/química
Lipossomas Unilamelares/metabolismo
p-Dimetilaminoazobenzeno/análogos & derivados
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Carrier Proteins); 0 (Fluorescent Dyes); 0 (Membrane Lipids); 0 (Phosphatidylcholines); 0 (Phosphatidylinositol Phosphates); 0 (Recombinant Fusion Proteins); 0 (Unilamellar Liposomes); 0 (enhanced green fluorescent protein); 0 (phosphatidylinositol 3,4,5-triphosphate); 147336-22-9 (Green Fluorescent Proteins); A49L8E13FD (p-Dimethylaminoazobenzene); EC 2.5.1.18 (Glutathione Transferase); EC 2.7.11.1 (AKT1 protein, human); EC 2.7.11.1 (Proto-Oncogene Proteins c-akt); TE895536Y5 (1-palmitoyl-2-oleoylphosphatidylcholine)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170811
[Lr] Data última revisão:
170811
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170109
[St] Status:MEDLINE


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[PMID]:26806100
[Au] Autor:Pan ML; Mukherjee MT; Patel HH; Patel B; Constantinescu CC; Mirbolooki MR; Liang C; Mukherjee J
[Ad] Endereço:Preclinical Imaging, Department of Radiological Sciences, University of California, Irvine, California, 92697.
[Ti] Título:Evaluation of [11C]TAZA for amyloid ß plaque imaging in postmortem human Alzheimer's disease brain region and whole body distribution in rodent PET/CT.
[So] Source:Synapse;70(4):163-76, 2016 Apr.
[Is] ISSN:1098-2396
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: Alzheimer's disease (AD) is a neurodegenerative disease characterized by Aß plaques in the brain. The aim of this study was to evaluate the effectiveness of a novel radiotracer, 4-[(11) C]methylamino-4'-N,N-dimethylaminoazobenzene ([(11)C]TAZA), for binding to Aß plaques in postmortem human brain (AD and normal control (NC)). METHODS: Radiosyntheses of [(11)C]TAZA, related [(11)C]Dalene ((11)C-methylamino-4'-dimethylaminostyrylbenzene), and reference [(11)C]PIB were carried out using [(11)C]methyltriflate prepared from [(11) C]CO(2) and purified using HPLC. In vitro binding affinities were carried out in human AD brain homogenate with Aß plaques labeled with [(3) H]PIB. In vitro autoradiography studies with the three radiotracers were performed on hippocampus of AD and NC brains. PET/CT studies were carried out in normal rats to study brain and whole body distribution. RESULTS: The three radiotracers were produced in high radiochemical yields (>40%) and had specific activities >37 GBq/µmol. TAZA had an affinity, K(i) = 0.84 nM and was five times more potent than PIB. [(11)C]TAZA bound specifically to Aß plaques present in AD brains with gray matter to white matter ratios >20. [(11)C]TAZA was displaced by PIB (>90%), suggesting similar binding site for [(11)C]TAZA and [(11)C]PIB. [(11)C]TAZA exhibited slow kinetics of uptake in the rat brain and whole body images showed uptake in interscapular brown adipose tissue (IBAT). Binding in brain and IBAT were affected by preinjection of atomoxetine, a norepinephrine transporter blocker. CONCLUSION: [(11)C]TAZA exhibited high binding to Aß plaques in human AD hippocampus. Rat brain kinetics was slow and peripheral binding to IBAT needs to be further evaluated.
[Mh] Termos MeSH primário: Doença de Alzheimer/diagnóstico por imagem
Placa Amiloide/diagnóstico por imagem
Tomografia por Emissão de Pósitrons
Compostos Radiofarmacêuticos/farmacocinética
p-Dimetilaminoazobenzeno/análogos & derivados
[Mh] Termos MeSH secundário: Animais
Benzotiazóis/farmacocinética
Hipocampo/diagnóstico por imagem
Seres Humanos
Masculino
Imagem Multimodal
Ligação Proteica
Compostos Radiofarmacêuticos/síntese química
Ratos
Ratos Sprague-Dawley
Especificidade da Espécie
Distribuição Tecidual
Tomografia Computadorizada por Raios X
Imagem Corporal Total
p-Dimetilaminoazobenzeno/síntese química
p-Dimetilaminoazobenzeno/farmacocinética
[Pt] Tipo de publicação:COMPARATIVE STUDY; EVALUATION STUDIES; JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (4-methylamino-4'-N,N-dimethylaminoazobenzene); 0 (Benzothiazoles); 0 (N-methyl-2-(4'-methylaminophenyl)-6-hydroxybenzothiazole); 0 (Radiopharmaceuticals); A49L8E13FD (p-Dimethylaminoazobenzene)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:170321
[Lr] Data última revisão:
170321
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160126
[St] Status:MEDLINE
[do] DOI:10.1002/syn.21893


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[PMID]:26287560
[Au] Autor:Zhuang Y; Zhang M; Chen B; Duan R; Min X; Zhang Z; Zheng F; Liang H; Zhao Z; Lou X; Xia F
[Ad] Endereço:Key Laboratory for Large-Format Battery Materials and System, Ministry of Education, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology , Wuhan 430074, China.
[Ti] Título:Quencher group induced high specificity detection of telomerase in clear and bloody urines by AIEgens.
[So] Source:Anal Chem;87(18):9487-93, 2015 Sep 15.
[Is] ISSN:1520-6882
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Telomerase is a widely used tumor biomarker for early cancer diagnosis. On the basis of the combined use of aggregation-induced emission (AIE) fluorogens and quencher, a quencher group induced high specificity strategy for detection of telomerase activity from cell extracts and cancer patients' urine specimens was creatively developed. In the absence of telomerase, fluorescence background is extremely low due to the short distance between quencher and AIE dye. In the addition of telomerase, fluorescence enhances significantly. The telomerase activity in the E-J, MCF-7, and HeLa extracts equivalent to 5-10 000 cells can be detected by this method in ∼1 h. Furthermore, the distinguishing of telomerase extracted from 38 cancer and 15 normal urine specimens confirms the reliability and practicality of this protocol. In contrast to our previous results (Anal. Chem. 2015, 87, 6822-6827), these advanced experiments obtain more remarkable specificity.
[Mh] Termos MeSH primário: Hematúria/urina
Limite de Detecção
Telomerase/urina
Urinálise/métodos
[Mh] Termos MeSH secundário: Linhagem Celular Tumoral
Corantes Fluorescentes/química
Hematúria/complicações
Hematúria/enzimologia
Seres Humanos
Espectrometria de Fluorescência
Neoplasias da Bexiga Urinária/complicações
Neoplasias da Bexiga Urinária/diagnóstico
Neoplasias da Bexiga Urinária/enzimologia
Neoplasias da Bexiga Urinária/urina
p-Dimetilaminoazobenzeno/análogos & derivados
p-Dimetilaminoazobenzeno/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Fluorescent Dyes); 6268-49-1 (4-(4-dimethylaminophenylazo)benzoic acid); A49L8E13FD (p-Dimethylaminoazobenzene); EC 2.7.7.49 (Telomerase)
[Em] Mês de entrada:1603
[Cu] Atualização por classe:150915
[Lr] Data última revisão:
150915
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150820
[St] Status:MEDLINE
[do] DOI:10.1021/acs.analchem.5b02699


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[PMID]:25892623
[Au] Autor:Shimada Y; Sui H; Wako Y; Kawasako K
[Ad] Endereço:Central Research Laboratories (Lab. 1), Hokko Chemical Industry Co. Ltd., 2165 Toda, Atsugi-shi, Kanagawa 243-0023, Japan. Electronic address: shimada-y@hokkochem.co.jp.
[Ti] Título:Evaluation of the repeated-dose liver micronucleus assay with p-dimethylaminoazobenzene.
[So] Source:Mutat Res Genet Toxicol Environ Mutagen;780-781:56-9, 2015 Mar.
[Is] ISSN:1879-3592
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The micronucleus induction by p-dimethylaminoazobenzene (DAB), a genotoxic rat liver carcinogen, was assessed in the liver and bone marrow of young adult rats after the repeated administration of DAB for 14 (Lab. 1) and 28 (Lab. 2) days. Three dose levels, 25, 50 and 100mg/kg/day, were used for the investigations in both labs. The frequency of micronucleated hepatocytes was significantly increased in a dose-dependent manner after the repeated administration of DAB at 50mg/kg/day or more for 14 and 28 days. Similarly, the frequency of micronucleated immature erythrocytes in the bone marrow was increased after the repeated administration of DAB at 100mg/kg/day for 14 and 28 days. These results indicate that the repeated-dose liver micronucleus assay allowed for the detection of micronucleus induction by DAB, and that the lowest detectable dose for micronucleus induction in the liver was lower than in the bone marrow. Thus, the repeated-dose liver micronucleus assay using young adult rats is considered suitable for the detection of micronucleus induction by liver carcinogens, such as DAB.
[Mh] Termos MeSH primário: Carcinógenos/toxicidade
Hepatócitos/efeitos dos fármacos
Fígado/efeitos dos fármacos
Testes para Micronúcleos
p-Dimetilaminoazobenzeno/toxicidade
[Mh] Termos MeSH secundário: Administração Oral
Animais
Peso Corporal/efeitos dos fármacos
Medula Óssea/efeitos dos fármacos
Aberrações Cromossômicas/efeitos dos fármacos
Comportamento Cooperativo
Relação Dose-Resposta a Droga
Esquema de Medicação
Hepatócitos/patologia
Seres Humanos
Japão
Fígado/patologia
Masculino
Especificidade de Órgãos
Ratos
Ratos Sprague-Dawley
Reticulócitos/efeitos dos fármacos
Sociedades Farmacêuticas
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Carcinogens); A49L8E13FD (p-Dimethylaminoazobenzene)
[Em] Mês de entrada:1506
[Cu] Atualização por classe:150420
[Lr] Data última revisão:
150420
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150421
[St] Status:MEDLINE


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[PMID]:25850198
[Au] Autor:Hubicka U; Zuromska-Witek B; Piotrowska J; Krzek J
[Ti] Título:Determination of neomycin in the form of neomycin derivative with dabsyl chloride by thin layer chromatography and densitometry.
[So] Source:Acta Pol Pharm;72(1):31-7, 2015 Jan-Feb.
[Is] ISSN:0001-6837
[Cp] País de publicação:Poland
[La] Idioma:eng
[Ab] Resumo:A thin layer chromatographic-densitometric method has been developed for identification and quantitative determination of neomycin derivative with dabsyl chloride. The analysis of antibiotic was achieved on the silica gel TLC plates with fluorescent indicator with n-butanol--2-butanone--25% ammonia--water (10 : 6 : 2 : 2, v/v/v/v) as the mobile phase. The densitometric measurements were made at 460 nm. Under these conditions good separation of chosen aminoglycoside antibiotic from reagent used to make a complex was obtained. The method is characterized by high sensitivity, LOD from 0.1953 µg per band and LOQ from 0.5918 µg per band, wide linearity range from 0.5918 to 2.1960 µg per band for neomycin. The precision of the method was good; RSD varied from 1.17 to 2.05%. Satisfactory results of validation of the method were also confirmed by determination of selected antibiotic in pharmaceutical commercial preparation. The results obtained by TLC-densitometric method were compared with those obtained by spectrophotometric method.
[Mh] Termos MeSH primário: Neomicina/química
p-Dimetilaminoazobenzeno/análogos & derivados
[Mh] Termos MeSH secundário: Antibacterianos/química
Cromatografia em Camada Delgada/métodos
Densitometria/métodos
Indicadores e Reagentes/química
Espectrofotometria/métodos
p-Dimetilaminoazobenzeno/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Indicators and Reagents); 1404-04-2 (Neomycin); 56512-49-3 (4-N,N-dimethylaminoazobenzene-4'-sulfonyl chloride); A49L8E13FD (p-Dimethylaminoazobenzene)
[Em] Mês de entrada:1504
[Cu] Atualização por classe:150408
[Lr] Data última revisão:
150408
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150409
[St] Status:MEDLINE


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[PMID]:25818461
[Au] Autor:Li Y; Huang W; You L; Xie T; He B
[Ad] Endereço:College of Basic Medical Science, Guiyang Medical University, Guiyang, Guizhou 550004, China.
[Ti] Título:A FRET-based assay for screening SIRT5 specific modulators.
[So] Source:Bioorg Med Chem Lett;25(8):1671-1674, 2015 Apr 15.
[Is] ISSN:1464-3405
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A fluorogenic assay for SIRT5 has been developed to screen their small molecule modulators based on the recent discovery that SIRT5 is a demalonylase and desuccinylase. However, this assay uses a fluorogenic peptide containing 7-amino-4-methylcoumarin (AMC), which becomes the cause of false positive hits from the screening. To overcome this, we have developed an alternative method called a FRET-based assay, which will be reliable and useful for screening SIRT5 modulators in a high-throughput format since no AMC group present in this assay.
[Mh] Termos MeSH primário: Transferência Ressonante de Energia de Fluorescência
Corantes Fluorescentes/química
Sirtuínas/metabolismo
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Cumarínicos/química
Ensaios Enzimáticos
Inibidores Enzimáticos/química
Inibidores Enzimáticos/metabolismo
Cinética
Dados de Sequência Molecular
Peptídeos/química
Peptídeos/metabolismo
Sirtuínas/antagonistas & inibidores
Especificidade por Substrato
p-Dimetilaminoazobenzeno/análogos & derivados
p-Dimetilaminoazobenzeno/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Coumarins); 0 (Enzyme Inhibitors); 0 (Fluorescent Dyes); 0 (Peptides); 6268-49-1 (4-(4-dimethylaminophenylazo)benzoic acid); A49L8E13FD (p-Dimethylaminoazobenzene); EC 3.5.1.- (Sirtuins); OCY3JCT44X (7-amino-4-methylcoumarin)
[Em] Mês de entrada:1512
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150331
[St] Status:MEDLINE


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[PMID]:25648065
[Au] Autor:Wang M; Zhang G; Zhang D
[Ad] Endereço:Beijing National Laboratory for Molecular Sciences, Organic Solids Laboratory, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China. dqzhang@iccas.ac.cn.
[Ti] Título:Enzyme-driven i-motif DNA folding for logic operations and fluorescent biosensing.
[So] Source:Chem Commun (Camb);51(18):3812-5, 2015 Mar 04.
[Is] ISSN:1364-548X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:DNA nanodevices capable of "NOR" and "NAND" logic operations were developed using enzymatic reactions to generate an acidic pH gradient and drive the conformation change of cytosine-rich DNA. Due to the high selectivity and sensitivity of the enzymatic reactions in driving DNA logic gates, novel fluorescent biosensors were further designed for enzyme activity assay and glucose sensing.
[Mh] Termos MeSH primário: Técnicas Biossensoriais
DNA/química
Lógica
Nanoestruturas/química
Motivos de Nucleotídeos
[Mh] Termos MeSH secundário: Acetilcolinesterase/química
Citosina/química
Fluorescência
Transferência Ressonante de Energia de Fluorescência
Corantes Fluorescentes/química
Glucose/análise
Glucose Oxidase/química
Concentração de Íons de Hidrogênio
Rodaminas/química
p-Dimetilaminoazobenzeno/análogos & derivados
p-Dimetilaminoazobenzeno/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Fluorescent Dyes); 0 (Rhodamines); 6268-49-1 (4-(4-dimethylaminophenylazo)benzoic acid); 8J337D1HZY (Cytosine); 9007-49-2 (DNA); A49L8E13FD (p-Dimethylaminoazobenzene); EC 1.1.3.4 (Glucose Oxidase); EC 3.1.1.7 (Acetylcholinesterase); IY9XDZ35W2 (Glucose)
[Em] Mês de entrada:1510
[Cu] Atualização por classe:150218
[Lr] Data última revisão:
150218
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150205
[St] Status:MEDLINE
[do] DOI:10.1039/c4cc09905b


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[PMID]:25315775
[Au] Autor:Gahl RF; He Y; Yu S; Tjandra N
[Ad] Endereço:From the Laboratory of Molecular Biophysics, Biochemistry and Biophysics Center, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892.
[Ti] Título:Conformational rearrangements in the pro-apoptotic protein, Bax, as it inserts into mitochondria: a cellular death switch.
[So] Source:J Biol Chem;289(47):32871-82, 2014 Nov 21.
[Is] ISSN:1083-351X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The B-cell lymphoma 2 (Bcl-2) family of proteins regulates the activation of apoptosis through the mitochondria pathway. Pro- and anti-apoptotic members of this family keep each other in check until the correct time to commit to apoptosis. The point of no return for this commitment is the permeabilization of the outer mitochondrial membrane. Translocation of the pro-apoptotic member, Bax, from the cytosol to the mitochondria is the molecular signature of this event. We employed a novel method to reliably detect Förster resonance energy transfer (FRET) between pairs of fluorophores to identify intra-molecular conformational changes and inter-molecular contacts in Bax as this translocation occurs in live cells. In the cytosol, our FRET measurement indicated that the C-terminal helix is exposed instead of tucked away in the core of the protein. In addition fluorescence correlation spectroscopy (FCS) showed that cytosolic Bax diffuses much slower than expected, suggesting possible complex formation or transient membrane interaction. Cross-linking the C-terminal helix (α9) to helix α4 reduced the potential of those interactions to occur. After translocation, our FRET measurements showed that Bax molecules form homo-oligomers in the mitochondria through two distinct interfaces involving the BH3 domain (helix α2) and the C-terminal helix. These findings have implications for possible contacts with other Bcl-2 proteins necessary for the regulation of apoptosis.
[Mh] Termos MeSH primário: Mitocôndrias/metabolismo
Conformação Proteica
Proteína X Associada a bcl-2/química
Proteína X Associada a bcl-2/metabolismo
[Mh] Termos MeSH secundário: Animais
Apoptose/efeitos dos fármacos
Células Cultivadas
Citosol/metabolismo
Embrião de Mamíferos/citologia
Fibroblastos/citologia
Fibroblastos/metabolismo
Transferência Ressonante de Energia de Fluorescência
Camundongos
Modelos Moleculares
Mutação
Estrutura Secundária de Proteína
Transporte Proteico
Compostos de Quinolínio/química
Espectrometria de Fluorescência
Estaurosporina/farmacologia
Proteína X Associada a bcl-2/genética
p-Dimetilaminoazobenzeno/análogos & derivados
p-Dimetilaminoazobenzeno/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., INTRAMURAL
[Nm] Nome de substância:
0 (Alexa fluor 546); 0 (Quinolinium Compounds); 0 (bcl-2-Associated X Protein); 6268-49-1 (4-(4-dimethylaminophenylazo)benzoic acid); A49L8E13FD (p-Dimethylaminoazobenzene); H88EPA0A3N (Staurosporine)
[Em] Mês de entrada:1502
[Cu] Atualização por classe:161019
[Lr] Data última revisão:
161019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141016
[St] Status:MEDLINE
[do] DOI:10.1074/jbc.M114.593897



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