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Pesquisa : D02.092.146.755 [Categoria DeCS]
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[PMID]:27771151
[Au] Autor:Susarla SM; Mulliken JB; Kaban LB; Manson PN; Dodson TB
[Ad] Endereço:Department of Oral and Maxillofacial Surgery, University of Washington School of Dentistry, Seattle, WA, USA; Division of Plastic and Craniofacial Surgery, Seattle Children's Hospital, Seattle, WA, USA. Electronic address: ssusarla@uw.edu.
[Ti] Título:The colourful history of malachite green: from ancient Egypt to modern surgery.
[So] Source:Int J Oral Maxillofac Surg;46(3):401-403, 2017 03.
[Is] ISSN:1399-0020
[Cp] País de publicação:Denmark
[La] Idioma:eng
[Mh] Termos MeSH primário: Anormalidades Craniofaciais/história
Anormalidades Craniofaciais/cirurgia
Corantes de Rosanilina/história
[Mh] Termos MeSH secundário: Egito
História do Século XX
História do Século XXI
História Antiga
Seres Humanos
Corantes de Rosanilina/química
[Pt] Tipo de publicação:HISTORICAL ARTICLE; LETTER
[Nm] Nome de substância:
0 (Rosaniline Dyes); 12058M7ORO (malachite green)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:D; IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE


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[PMID]:28461340
[Au] Autor:Fusco R; Gugliandolo E; Biundo F; Campolo M; Di Paola R; Cuzzocrea S
[Ad] Endereço:Department of Chemical, Biological, Pharmaceutical, and Environmental Sciences, University of Messina, Messina, Italy.
[Ti] Título:Inhibition of inflammasome activation improves lung acute injury induced by carrageenan in a mouse model of pleurisy.
[So] Source:FASEB J;31(8):3497-3511, 2017 08.
[Is] ISSN:1530-6860
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The inflammasome NLRP3 is a molecular pathway activated by a wide range of cellular insults to elicit innate immune defenses through the activation of caspase-1 and the maturation of proinflammatory cytokines, such as IL-1ß and IL-18. The expression of NRLP3 is abnormally elevated in numerous human inflammatory diseases, including pulmonary diseases. An injection of carrageenan (CAR) into the pleural cavity triggered an acute inflammatory response, leading to tissue damage, inflammatory exudates, leukocyte infiltration, and increased myeloperoxidase activity. The aim of this study was to assess the effect of the inflammasome blocking agents BAY 11-7082 (30 mg/kg, i.p.) and Brilliant Blue G (BBG) (45.5 mg/kg, i.p.) in a mouse model of CAR-induced pleurisy. Treatment with BAY 11-7082 or BBG 1 h after CAR injection attenuated pulmonary membrane thickening and polymorphonuclear leukocyte infiltration, reduced NF-κB translocation in the nucleus, and inhibited the assembly of the NRLP3/ASC/caspase-1 complex. Treatment with BAY 11-7082 or BBG also down-regulated iNOS, nitrotyrosine, and poly-ADP-ribosyl polymerase expression and inhibited CAR-induced apoptosis. Our results demonstrate that treatment with inflammasome-blocking agents can significantly reduce the development of acute CAR-induced lung injury.-Fusco, R. Gugliandolo, E., Biundo, F., Campolo, M., Di Paola, R., Cuzzocrea, S. Inhibition of inflammasome activation improves lung acute injury induced by carrageenan in a mouse model of pleurisy.
[Mh] Termos MeSH primário: Lesão Pulmonar Aguda/induzido quimicamente
Carragenina/toxicidade
Regulação da Expressão Gênica/efeitos dos fármacos
Inflamassomos/metabolismo
Pleurisia/induzido quimicamente
[Mh] Termos MeSH secundário: Lesão Pulmonar Aguda/metabolismo
Lesão Pulmonar Aguda/prevenção & controle
Animais
Citocinas/genética
Citocinas/metabolismo
Inflamassomos/efeitos dos fármacos
Inflamassomos/genética
Masculino
Camundongos
Fator 2 Relacionado a NF-E2/genética
Fator 2 Relacionado a NF-E2/metabolismo
Proteína 3 que Contém Domínio de Pirina da Família NLR/antagonistas & inibidores
Proteína 3 que Contém Domínio de Pirina da Família NLR/genética
Proteína 3 que Contém Domínio de Pirina da Família NLR/metabolismo
Nitrilos/farmacologia
Pleurisia/metabolismo
Pleurisia/prevenção & controle
Corantes de Rosanilina/farmacologia
Sulfonas/farmacologia
Superóxido Dismutase/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (3-(4-methylphenylsulfonyl)-2-propenenitrile); 0 (Cytokines); 0 (Inflammasomes); 0 (NF-E2-Related Factor 2); 0 (NLR Family, Pyrin Domain-Containing 3 Protein); 0 (Nfe2l2 protein, mouse); 0 (Nitriles); 0 (Rosaniline Dyes); 0 (Sulfones); 9000-07-1 (Carrageenan); EC 1.15.1.1 (Superoxide Dismutase); M1ZRX790SI (coomassie Brilliant Blue)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:180206
[Lr] Data última revisão:
180206
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE
[do] DOI:10.1096/fj.201601349R


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[PMID]:28448526
[Au] Autor:Pettengill M; Matute JD; Tresenriter M; Hibbert J; Burgner D; Richmond P; Luis Millán J; Ozonoff A; Strunk T; Currie A; Levy O
[Ad] Endereço:Precision Vaccines Program, Division of Infectious Diseases, Boston Children's Hospital, Boston, Massachusetts, United States of America.
[Ti] Título:Human alkaline phosphatase dephosphorylates microbial products and is elevated in preterm neonates with a history of late-onset sepsis.
[So] Source:PLoS One;12(4):e0175936, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: A host defense function for Alkaline phosphatases (ALPs) is suggested by the contribution of intestinal ALP to detoxifying bacterial lipopolysaccharide (endotoxin) in animal models in vivo and the elevation of ALP activity following treatment of human cells with inflammatory stimuli in vitro. However the activity of ALP in human plasma (primarily tissue-nonspecific ALP; TNAP) on lipopolysaccharide and other microbial products has not been assessed, nor has its expression been studied in preterm newborns, a vulnerable population at high risk of sepsis. In this context, the aim of our study was to characterize the activity of TNAP on Toll-like receptor (TLR) agonists and assess the concentrations of plasma ALP during late-onset sepsis in preterm newborns. METHODS: Recombinant human TNAP was incubated with microbial products and phosphate release was measured by malachite green assay. Plasma ALP activity was measured serially in a cohort of preterm (N = 129) infants at high risk of late-onset sepsis (LOS). RESULTS: TNAP dephosphorylates poly-inosine:cytosine (Toll-like receptor (TLR) 3 agonist) and LPS from Klebsiella pneumoniae and Salmonella minnesota (TLR4 agonists). Plasma ALP significantly increased postnatally over the first 4 weeks of life in preterm and term newborns. Bacteremic LOS in preterm infants (gestational age ≤ 30 weeks) was associated with significantly elevated plasma ALP at 4 weeks postnatal age. CONCLUSIONS: TNAP, the main circulating isozyme of ALP, de-phosphorylates TLR agonists, demonstrates a post-natal age dependent increase in preterm and term plasma across the first 4 weeks of life, and is elevated in association with preterm LOS.
[Mh] Termos MeSH primário: Fosfatase Alcalina/metabolismo
Lipopolissacarídeos/metabolismo
Sepse/diagnóstico
[Mh] Termos MeSH secundário: Fosfatase Alcalina/sangue
Fosfatase Alcalina/genética
Ensaios Enzimáticos
Feminino
Idade Gestacional
Seres Humanos
Lactente
Recém-Nascido
Recém-Nascido Prematuro
Isoenzimas/sangue
Isoenzimas/metabolismo
Klebsiella pneumoniae/metabolismo
Transtornos de Início Tardio
Masculino
Fosfatos/análise
Fosfatos/química
Proteínas Recombinantes/isolamento & purificação
Proteínas Recombinantes/metabolismo
Corantes de Rosanilina/química
Corantes de Rosanilina/metabolismo
Salmonella/metabolismo
Sepse/microbiologia
Sepse/mortalidade
Receptores Toll-Like/agonistas
Receptores Toll-Like/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Isoenzymes); 0 (Lipopolysaccharides); 0 (Phosphates); 0 (Recombinant Proteins); 0 (Rosaniline Dyes); 0 (Toll-Like Receptors); 12058M7ORO (malachite green); EC 3.1.3.1 (Alkaline Phosphatase)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:180125
[Lr] Data última revisão:
180125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170428
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0175936


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[PMID]:28802653
[Au] Autor:Nebbia C; Girolami F; Carletti M; Gasco L; Zoccarato I; Giuliano Albo A
[Ad] Endereço:Department of Veterinary Sciences, University of Torino, Grugliasco, Italy. Electronic address: carlo.nebbia@unito.it.
[Ti] Título:In vitro interactions of malachite green and leucomalachite green with hepatic drug-metabolizing enzyme systems in the rainbow trout (Onchorhyncus mykiss).
[So] Source:Toxicol Lett;280:41-47, 2017 Oct 05.
[Is] ISSN:1879-3169
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Malachite green (MG) has been widely used in aquaculture to treat a number of microbial and parasitic diseases. It is currently banned in the EU because of the high cytotoxicity and carcinogenic activity, which is also shared by leucomalachite green (LMG), a reduced MG metabolite that can persist in fish tissues for months. There is scant information about the ability of either compound to interact with drug metabolizing enzymes in fish. Therefore we evaluated the in vitro effects of MG and LMG (25, 50 and 100µM) on some DMEs and glutathione (GSH) content in rainbow trout liver subfractions. LMG did not affect any of the examined parameters. In contrast, MG proved to deplete GSH and to depress to a various extent the activities of NAD(P)H cytochrome c reductase, 7-ethoxycoumarin O-deethylase, 1-naphthol uridindiphosphoglucuronyl-transferase and maximally those of 7-ethoxyresorufin O-deethylase (EROD) and glutathione S-transferase (GST) accepting 1-chloro2,4-dinitrobenzene (CDNB) as substrate. The inhibition mechanisms of EROD and GST were investigated by means of non-linear Michaelis-Menten kinetics and Lineweaver-Burk plots using 0.175-8µM MG. The calculated IC for EROD was 7.1µM, and the inhibition appeared to be competitive (K 2.78±0.24µM). In the case of GST, the calculated IC was 0.53µM. The inhibition was best described as competitive toward GSH (Ki 0.39±0.02µM) and of mixed-type toward CDNB (Ki 0.64±0.06µM). Our findings indicate that, contrary to LMG, MG behaves as a relatively strong inhibitor of certain liver DMEs and can reversibly bind GSH.
[Mh] Termos MeSH primário: Regulação Enzimológica da Expressão Gênica/efeitos dos fármacos
Fígado/enzimologia
Corantes de Rosanilina/toxicidade
[Mh] Termos MeSH secundário: Animais
Anti-Infecciosos Locais/química
Anti-Infecciosos Locais/toxicidade
Feminino
Masculino
Oncorhynchus mykiss
Corantes de Rosanilina/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RANDOMIZED CONTROLLED TRIAL
[Nm] Nome de substância:
0 (Anti-Infective Agents, Local); 0 (Rosaniline Dyes); 12058M7ORO (malachite green); 8U61G37Z20 (leucomalachite green)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171017
[Lr] Data última revisão:
171017
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170814
[St] Status:MEDLINE


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[PMID]:28711019
[Au] Autor:Baghbani-Arani F; Movagharnia R; Sharifian A; Salehi S; Shandiz SAS
[Ad] Endereço:Department of Genetics and Biotechnology, School of Biological Science, Varamin-Pishva Branch, Islamic Azad University, Varamin, Iran.
[Ti] Título:Photo-catalytic, anti-bacterial, and anti-cancer properties of phyto-mediated synthesis of silver nanoparticles from Artemisia tournefortiana Rchb extract.
[So] Source:J Photochem Photobiol B;173:640-649, 2017 Aug.
[Is] ISSN:1873-2682
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Metal nanoparticles have largely been investigated due to their potential medicinal activities. This study demonstrates the biological properties of green-synthesized silver nanoparticles (AgNPs) by using Artemisia tournefortiana Rchb ethanol extract. Instrumentations such as ultraviolet-visible spectra analysis, high-resolution transmission electron microscopy, scanning electron microscopy, energy-dispersive X-ray analysis, X-ray diffraction, and Fourier transform infrared spectroscopy were used to reveal the synthesized AgNPs. Microscopic results showed that the particles were mostly spherical in shape, having an average diameter of 22.89±14.82nm. The antibacterial activity of the phyto-fabricated AgNPs was investigated by the determination of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The in vitro cytotoxicity effect was investigated against normal human embryonic kidney (HEK293) cells and human colon adenocarcinoma cancer (HT29) cells. The apoptotic cells were identified by annexin V/PI FITC staining, and morphological assessment. The expressions of Bax and Bcl2 were evaluated by quantitative real time PCR method. The phyto-synthesized AgNPs have shown increased cell apoptosis and demonstrated dose-dependent cytotoxicity in HT29 cancer cells. Moreover, the photocatalytic activity of the phyto-synthesized AgNPs was evaluated by degradation of Coomassie Brilliant Blue G-250 under UV light exposure and these fabricated Ag nanoparticles demonstrated efficacy in degrading the dye within 60min. Overall, the present results highlighted the antibacterial and anticancer properties of fabricated AgNPs, suggesting that phyto-synthesized silver nanoparticles could possess potent anti-pathogenic bacteria and anti-colon cancer activities.
[Mh] Termos MeSH primário: Antibacterianos/síntese química
Antineoplásicos/síntese química
Artemisia/química
Nanopartículas Metálicas/química
Prata/química
[Mh] Termos MeSH secundário: Antibacterianos/química
Antibacterianos/farmacologia
Antineoplásicos/química
Antineoplásicos/toxicidade
Artemisia/metabolismo
Catálise
Sobrevivência Celular/efeitos dos fármacos
Expressão Gênica/efeitos dos fármacos
Bactérias Gram-Negativas/efeitos dos fármacos
Bactérias Gram-Positivas/efeitos dos fármacos
Química Verde
Células HEK293
Células HT29
Seres Humanos
Nanopartículas Metálicas/uso terapêutico
Nanopartículas Metálicas/toxicidade
Testes de Sensibilidade Microbiana
Microscopia Eletrônica de Transmissão
Microscopia de Fluorescência
Fotólise/efeitos da radiação
Extratos Vegetais/química
Folhas de Planta/química
Folhas de Planta/metabolismo
Proteínas Proto-Oncogênicas c-bcl-2/genética
Proteínas Proto-Oncogênicas c-bcl-2/metabolismo
Corantes de Rosanilina/química
Espectroscopia de Infravermelho com Transformada de Fourier
Raios Ultravioleta
Difração de Raios X
Proteína X Associada a bcl-2/genética
Proteína X Associada a bcl-2/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Antineoplastic Agents); 0 (Plant Extracts); 0 (Proto-Oncogene Proteins c-bcl-2); 0 (Rosaniline Dyes); 0 (bcl-2-Associated X Protein); 3M4G523W1G (Silver); M1ZRX790SI (coomassie Brilliant Blue)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171010
[Lr] Data última revisão:
171010
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170716
[St] Status:MEDLINE


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[PMID]:28669743
[Au] Autor:Caglayan B; Caglayan AB; Beker MC; Yalcin E; Beker M; Kelestemur T; Sertel E; Ozturk G; Kilic U; Sahin F; Kilic E
[Ad] Endereço:Istanbul Medipol University, Regenerative and Restorative Medical Research Center, Istanbul, Turkey; Istanbul Medipol University, Dept. of Physiology, Istanbul, Turkey; Yeditepe University, Dept. of Genetics and Bioengineering, Istanbul, Turkey.
[Ti] Título:Evidence that activation of P2X7R does not exacerbate neuronal death after optic nerve transection and focal cerebral ischemia in mice.
[So] Source:Exp Neurol;296:23-31, 2017 Oct.
[Is] ISSN:1090-2430
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Conflicting data in the literature about the function of P2X7R in survival following ischemia necessitates the conductance of in-depth studies. To investigate the impacts of activation vs inhibition of the receptor on neuronal survival as well as the downstream signaling cascades, in addition to optic nerve transection (ONT), 30min and 90min of middle cerebral artery occlusion (MCAo) models were performed in mice. Intracellular calcium levels were assessed in primary cortical neuron cultures. Here, we show that P2X7R antagonist Brilliant Blue G (BBG) decreased DNA fragmentation, infarct volume, brain swelling, neurological deficit scores and activation of microglial cells after focal cerebral ischemia. BBG also significantly increased the number of surviving retinal ganglion cells (RGCs) after ONT and the number of surviving neurons following MCAo. Importantly, receptor agonist BzATP resulted in increased activation of microglial cells and induced phosphorylation of ERK, AKT and JNK. These results indicated that inhibition of P2X7R with BBG promoted neuronal survival, not through the activation of survival kinase pathways, but possibly by improved intracellular Ca overload and decreased the levels of Caspase 1, IL-1ß and Bax proteins. On the other hand, BzATP-mediated increased number of activated microglia and increased survival kinase levels in addition to increased caspase-1 and IL-1ß levels indicate the complex nature of the P2X7 receptor-mediated signaling in neuronal injury.
[Mh] Termos MeSH primário: Isquemia Encefálica/metabolismo
Isquemia Encefálica/patologia
Neurônios/patologia
Traumatismos do Nervo Óptico/metabolismo
Traumatismos do Nervo Óptico/patologia
Receptores Purinérgicos P2/metabolismo
[Mh] Termos MeSH secundário: Trifosfato de Adenosina/análogos & derivados
Trifosfato de Adenosina/farmacologia
Trifosfato de Adenosina/uso terapêutico
Animais
Animais Recém-Nascidos
Encéfalo/irrigação sanguínea
Encéfalo/efeitos dos fármacos
Edema Encefálico/etiologia
Isquemia Encefálica/tratamento farmacológico
Proteínas de Ligação ao Cálcio/metabolismo
Células Cultivadas
Córtex Cerebral/citologia
Citocinas/metabolismo
Fragmentação do DNA/efeitos dos fármacos
Modelos Animais de Doenças
Infusões Intraventriculares
Masculino
Camundongos
Camundongos Endogâmicos C57BL
Proteínas dos Microfilamentos/metabolismo
Neurônios/efeitos dos fármacos
Traumatismos do Nervo Óptico/tratamento farmacológico
Inibidores da Agregação de Plaquetas/farmacologia
Inibidores da Agregação de Plaquetas/uso terapêutico
Corantes de Rosanilina/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Aif1 protein, mouse); 0 (Calcium-Binding Proteins); 0 (Cytokines); 0 (Microfilament Proteins); 0 (Platelet Aggregation Inhibitors); 0 (Receptors, Purinergic P2); 0 (Rosaniline Dyes); 0 (purinergic P2X8 receptor); 4P5DXU1F8Q (3'-O-(4-benzoyl)benzoyladenosine 5'-triphosphate); 8L70Q75FXE (Adenosine Triphosphate); M1ZRX790SI (coomassie Brilliant Blue)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170822
[Lr] Data última revisão:
170822
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170704
[St] Status:MEDLINE


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[PMID]:28665482
[Au] Autor:Geraghty NJ; Belfiore L; Ly D; Adhikary SR; Fuller SJ; Varikatt W; Sanderson-Smith ML; Sluyter V; Alexander SI; Sluyter R; Watson D
[Ad] Endereço:School of Biological Sciences, University of Wollongong, Wollongong, NSW, Australia.
[Ti] Título:The P2X7 receptor antagonist Brilliant Blue G reduces serum human interferon-γ in a humanized mouse model of graft-versus-host disease.
[So] Source:Clin Exp Immunol;190(1):79-95, 2017 Oct.
[Is] ISSN:1365-2249
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Graft-versus-host disease (GVHD) remains a major problem after allogeneic haematopoietic stem cell transplantation, a curative therapy for haematological malignancies. Previous studies have demonstrated a role for the adenosine triphosphate (ATP)-gated P2X7 receptor channel in allogeneic mouse models of GVHD. In this study, injection of human peripheral blood mononuclear cells (PBMCs) into immunodeficient non-obese diabetic-severe combined immunodeficiency-interleukin (NOD-SCID-IL)-2Rγ (NSG) mice established a humanized mouse model of GVHD. This model was used to study the effect of P2X7 blockade in this disease. From five weeks post-PBMC injection, humanized mice exhibited clinical signs and histopathology characteristic of GVHD. The P2X7 antagonist, Brilliant Blue G (BBG), blocked ATP-induced cation uptake into both murine and human cells in vitro. Injection of BBG (50 mg/kg) into NSG mice did not affect engraftment of human leucocytes (predominantly T cells), or the clinical score and survival of mice. In contrast, BBG injection reduced circulating human interferon (IFN)-γ significantly, which was produced by human CD4 and CD8 T cells. BBG also reduced human T cell infiltration and apoptosis in target organs of GVHD. In conclusion, the P2X7 antagonist BBG reduced circulating IFN-γ in a humanized mouse model of GVHD supporting a potential role for P2X7 to alter the pathology of this disease in humans.
[Mh] Termos MeSH primário: Linfócitos T CD4-Positivos/efeitos dos fármacos
Linfócitos T CD8-Positivos/efeitos dos fármacos
Doença Enxerto-Hospedeiro/tratamento farmacológico
Transplante de Células-Tronco Hematopoéticas
Interferon gama/sangue
Antagonistas do Receptor Purinérgico P2X/uso terapêutico
Corantes de Rosanilina/uso terapêutico
[Mh] Termos MeSH secundário: Animais
Apoptose/efeitos dos fármacos
Linfócitos T CD4-Positivos/imunologia
Linfócitos T CD8-Positivos/imunologia
Movimento Celular/efeitos dos fármacos
Células Cultivadas
Modelos Animais de Doenças
Doença Enxerto-Hospedeiro/imunologia
Seres Humanos
Subunidade gama Comum de Receptores de Interleucina/genética
Camundongos
Camundongos Endogâmicos NOD
Camundongos Knockout
Camundongos SCID
Receptores Purinérgicos P2X7/metabolismo
Transplante Homólogo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Il2rg protein, mouse); 0 (Interleukin Receptor Common gamma Subunit); 0 (Purinergic P2X Receptor Antagonists); 0 (Receptors, Purinergic P2X7); 0 (Rosaniline Dyes); 82115-62-6 (Interferon-gamma); M1ZRX790SI (coomassie Brilliant Blue)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171003
[Lr] Data última revisão:
171003
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170701
[St] Status:MEDLINE
[do] DOI:10.1111/cei.13005


  8 / 4115 MEDLINE  
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Laus, José Luiz
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[PMID]:28594698
[Au] Autor:Aldrovani M; Barros Sobrinho AAF; Mairos FS; Laus JL
[Ad] Endereço:Ophthalmology Unit, Department of Small Animal Medicine and Surgery, Sao Paulo State University (UNESP), School of Agricultural and Veterinarian Sciences, Jaboticabal, SP, Brazil.
[Ti] Título:Short-Term Effects of Y-27632, a Rho-Associated Protein Kinase Inhibitor, on Chromatin Supraorganization and DNA Amount in Epithelial Cells of the Rat Cornea and Limbus.
[So] Source:Cornea;36(7):845-853, 2017 Jul.
[Is] ISSN:1536-4798
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:PURPOSE: To assess the short-term effects of instilling Y-27632, an inhibitor of Rho/Rho-associated protein kinases, on the chromatin supraorganization and DNA amount of corneal and limbal epithelial cells of healthy rats. METHODS: Longitudinal sections (7 µm) of enucleated eyes of healthy rats that received, by instillation, balanced salt solution with or without 10 mM of Y-27632 daily for 7 or 15 days, were subjected to the Feulgen reaction. Feulgen-stained nuclei of corneal and limbal epithelial cells were studied by microscopy and video image analysis to establish the nuclear size (area and perimeter), supraorganization of chromatin (texture and degrees of condensation), and the Feulgen-DNA amount. RESULTS: Instillation of Y-27632 for up to 15 days did not change the size of the nucleus or the chromatin texture of corneal and limbal epithelial cells. Samples treated with Y-27632 for 7 days showed condensed chromatin and a high Feulgen-DNA amount. Both corneal and limbal epithelium showed the presence of near-tetraploid nuclei corresponding to cells in the S and G2 phases of the cell cycle. The degrees of condensation and Feulgen-DNA amount of the nuclei of epithelial cells of the cornea and limbus of eyes from rats receiving Y-27632 for 15 days did not differ from control (no drug). CONCLUSIONS: Changes in chromatin supraorganization and DNA amount, such as seen in this study, are indicative of cell proliferation and do not seem to be associated with disturbances in gene activity and transcription of DNA.
[Mh] Termos MeSH primário: Amidas/farmacologia
Cromatina/efeitos dos fármacos
DNA/metabolismo
Epitélio Anterior/metabolismo
Limbo da Córnea/citologia
Inibidores de Proteínas Quinases/farmacologia
Piridinas/farmacologia
Quinases Associadas a rho/antagonistas & inibidores
[Mh] Termos MeSH secundário: Animais
Núcleo Celular/efeitos dos fármacos
Proliferação Celular
Cromatina/metabolismo
Masculino
Soluções Oftálmicas
Ratos
Ratos Wistar
Corantes de Rosanilina
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amides); 0 (Chromatin); 0 (Feulgen stain); 0 (Ophthalmic Solutions); 0 (Protein Kinase Inhibitors); 0 (Pyridines); 0 (Rosaniline Dyes); 138381-45-0 (Y 27632); 9007-49-2 (DNA); EC 2.7.11.1 (rho-Associated Kinases)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170728
[Lr] Data última revisão:
170728
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170609
[St] Status:MEDLINE
[do] DOI:10.1097/ICO.0000000000001221


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[PMID]:28437742
[Au] Autor:Paz A; Carballo J; Pérez MJ; Domínguez JM
[Ad] Endereço:Chemical Engineering Department, Faculty of Sciences, University of Vigo (Campus Ourense), As Lagoas s/n, 32004, Ourense, Spain; Laboratory of Agro-food Biotechnology, CITI-University of Vigo, Tecnópole, Technological Park of Galicia, San Cibrao das Viñas, Ourense, Spain.
[Ti] Título:Biological treatment of model dyes and textile wastewaters.
[So] Source:Chemosphere;181:168-177, 2017 Aug.
[Is] ISSN:1879-1298
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Previous works conducted in our laboratory, reveled that Bacillus aryabhattai DC100 produce ligninolytic enzymes such as laccases and/or peroxidases, opening new applications in different bioprocesses, including the treatment of disposal residues such as dyestuffs from textile processing industries. This work described the degradation of three commercial model dyes Coomassie Brilliant Blue G-250 (CBB), Indigo Carmine (IC) and Remazol Brilliant Blue R (RBBR) under different culture media and operational conditions. The process was optimized using a Central Composite Rotatable Design, and the desirability predicted complete decolorization of 150 mg/L CBB at 37 °C, 304.09 rpm and salt concentration of 19.204 g/L. The model was validated with concentrations up to 180 mg/L CBB and IC, not being able to remove high amount of RBBR. The procedure here developed also allowed Chemical Oxygen Demands (COD) reductions in CBB of about 42%, meanwhile tests on real effluents from a local textile industry involved COD reductions of 50% in a liquid wastewater and 14% in semi-liquid sludge. Thus, allow the authorized discharge of wastewater into the corresponding treatment plant. Decolorization efficiencies and COD reductions open on the potential application of B. aryabhattai DC100 on the bioremediation of real effluents from textile industries.
[Mh] Termos MeSH primário: Biodegradação Ambiental
Corantes/metabolismo
Indústria Têxtil
Águas Residuais/microbiologia
[Mh] Termos MeSH secundário: Antraquinonas/metabolismo
Bacillus/enzimologia
Bacillus/metabolismo
Índigo Carmim/metabolismo
Lacase/metabolismo
Peroxidases/metabolismo
Corantes de Rosanilina/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anthraquinones); 0 (Coloring Agents); 0 (Rosaniline Dyes); 0 (Waste Water); D3741U8K7L (Indigo Carmine); EC 1.10.3.2 (Laccase); EC 1.11.1.- (Peroxidases); L51IMM9UP9 (Remazol Brilliant Blue R); M1ZRX790SI (coomassie Brilliant Blue)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170802
[Lr] Data última revisão:
170802
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170425
[St] Status:MEDLINE


  10 / 4115 MEDLINE  
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[PMID]:28435131
[Au] Autor:Abdelaziz HA; Shaker ME; Hamed MF; Gameil NM
[Ad] Endereço:Pharmacology and Toxicology Dept., Faculty of Pharmacy, Mansoura University, Mansoura 35516, Egypt; Pharmacology and Biochemistry Dept., Faculty of Pharmacy, Delta University for Science and Technology, Gamasa, Egypt.
[Ti] Título:Repression of acetaminophen-induced hepatotoxicity by a combination of celastrol and brilliant blue G.
[So] Source:Toxicol Lett;275:6-18, 2017 Jun 05.
[Is] ISSN:1879-3169
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The sterile inflammatory response is an eminent contributor to acetaminophen (APAP)-hepatotoxicity in humans. Recent advances unraveled an axial role of the NLRP3-inflammasome in APAP-post injury inflammation. Nevertheless, the role of signaling events preceded the NLRP3-inflammasome activation, like the transcription factor NF-κB and the purinergic receptor P2X7, is still unclear and needs further elucidation. Here, we investigated the pharmacological inhibition of these upstream signaling molecules by celastrol and brilliant blue G (BBG) (separately or simultaneously) in APAP-hepatotoxicity in mice. The results indicated that both celastrol and BBG pretreatments, especially when combined together, curbed APAP-induced hepatocellular injury (ALT, AST and LDH) and death (necrosis and apoptosis). The underlying mechanisms of protection of such combination against APAP-challenge were attributed to their efficient cooperation in: i) preventing the consumption of hepatic antioxidants (reduced glutathione and superoxide dismutase); ii) limiting the overproduction of lipid peroxidation aldehydes (malondialdehyde and 4-hydroxynonenal) and total nitrate/nitrite products; iii) attenuating the inflammatory cells accumulation in the liver, as evidenced by reducing the number of F4/80 positive cells/field in immunostaining and myeloperoxidase activity; iv) reversing the dysregulation in production of pro-inflammatory (TNF-α, IL-17A and IL-23) and anti-inflammatory (IL-10) cytokines; and v) enhancing the reparative capacity of injured hepatocytes, as demonstrated by increasing the percentage of PCNA positive hepatocytes per field of immunostaining. In conclusion, this murine study elicits a potential clinical applicability and therapeutic utility of celastrol and BBG combination in human cases of APAP-overdose hepatotoxicity.
[Mh] Termos MeSH primário: Acetaminofen/toxicidade
Anti-Inflamatórios/uso terapêutico
Doença Hepática Induzida por Substâncias e Drogas/prevenção & controle
Fígado/efeitos dos fármacos
Corantes de Rosanilina/uso terapêutico
Triterpenos/uso terapêutico
[Mh] Termos MeSH secundário: Animais
Anti-Inflamatórios/administração & dosagem
Antioxidantes/metabolismo
Doença Hepática Induzida por Substâncias e Drogas/etiologia
Doença Hepática Induzida por Substâncias e Drogas/imunologia
Doença Hepática Induzida por Substâncias e Drogas/patologia
Citocinas/imunologia
Quimioterapia Combinada
Ensaio de Imunoadsorção Enzimática
Fígado/imunologia
Fígado/patologia
Testes de Função Hepática
Masculino
Camundongos Endogâmicos BALB C
Estresse Oxidativo/efeitos dos fármacos
Corantes de Rosanilina/administração & dosagem
Triterpenos/administração & dosagem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents); 0 (Antioxidants); 0 (Cytokines); 0 (Rosaniline Dyes); 0 (Triterpenes); 362O9ITL9D (Acetaminophen); L8GG98663L (tripterine); M1ZRX790SI (coomassie Brilliant Blue)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170626
[Lr] Data última revisão:
170626
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170425
[St] Status:MEDLINE



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