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[PMID]:29184999
[Au] Autor:Oya M; Suzuki H; Anas ARJ; Oishi K; Ono K; Yamaguchi S; Eguchi M; Sawada M
[Ad] Endereço:Department of Brain Function, Division of Stress Adaptation and Protection, Research Institute of Environmental Medicine, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi, 464-8601, Japan.
[Ti] Título:LC-MS/MS imaging with thermal film-based laser microdissection.
[So] Source:Anal Bioanal Chem;410(2):491-499, 2018 Jan.
[Is] ISSN:1618-2650
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Mass spectrometry (MS) imaging is a useful tool for direct and simultaneous visualization of specific molecules. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is used to evaluate the abundance of molecules in tissues using sample homogenates. To date, however, LC-MS/MS has not been utilized as an imaging tool because spatial information is lost during sample preparation. Here we report a new approach for LC-MS/MS imaging using a thermal film-based laser microdissection (LMD) technique. To isolate tissue spots, our LMD system uses a 808-nm near infrared laser, the diameter of which can be freely changed from 2.7 to 500 µm; for imaging purposes in this study, the diameter was fixed at 40 µm, allowing acquisition of LC-MS/MS images at a 40-µm resolution. The isolated spots are arranged on a thermal film at 4.5-mm intervals, corresponding to the well spacing on a 384-well plate. Each tissue spot is handled on the film in such a manner as to maintain its spatial information, allowing it to be extracted separately in its individual well. Using analytical LC-MS/MS in combination with the spatial information of each sample, we can reconstruct LC-MS/MS images. With this imaging technique, we successfully obtained the distributions of pilocarpine, glutamate, γ-aminobutyric acid, acetylcholine, and choline in a cross-section of mouse hippocampus. The protocol we established in this study is applicable to revealing the neurochemistry of pilocarpine model of epilepsy. Our system has a wide range of uses in fields such as biology, pharmacology, pathology, and neuroscience. Graphical abstract Schematic Indication of LMD-LC-MS/MS imaging.
[Mh] Termos MeSH primário: Hipocampo/química
Microdissecção e Captura a Laser/métodos
Neurotransmissores/análise
Espectrometria de Massas em Tandem/métodos
[Mh] Termos MeSH secundário: Acetilcolina/análise
Animais
Colina/análise
Cromatografia Líquida/métodos
Epilepsia/diagnóstico
Epilepsia/patologia
Feminino
Ácido Glutâmico/análise
Hipocampo/patologia
Camundongos Endogâmicos C57BL
Pilocarpina/análise
Ácido gama-Aminobutírico/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Neurotransmitter Agents); 01MI4Q9DI3 (Pilocarpine); 3KX376GY7L (Glutamic Acid); 56-12-2 (gamma-Aminobutyric Acid); N91BDP6H0X (Choline); N9YNS0M02X (Acetylcholine)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171130
[St] Status:MEDLINE
[do] DOI:10.1007/s00216-017-0739-2


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[PMID]:28462392
[Au] Autor:Li WC; Zhu XY; Ritson E
[Ad] Endereço:University of St Andrews, St Andrews, Fife KY16 9JP, Scotland.
[Ti] Título:Mechanosensory Stimulation Evokes Acute Concussion-Like Behavior by Activating GIRKs Coupled to Muscarinic Receptors in a Simple Vertebrate.
[So] Source:eNeuro;4(2), 2017 Mar-Apr.
[Is] ISSN:2373-2822
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Most vertebrates show concussion responses when their heads are hit suddenly by heavy objects. Previous studies have focused on the direct physical injuries to the neural tissue caused by the concussive blow. We study a similar behavior in a simple vertebrate, the tadpole. We find that concussion-like behavior can be reliably induced by the mechanosensory stimulation of the head skin without direct physical impacts on the brain. Head skin stimulation activates a cholinergic pathway which then opens G protein-coupled inward-rectifying potassium channels (GIRKs) via postsynaptic M muscarinic receptors to inhibit brainstem neurons critical for the initiation and maintenance of swimming for up to minutes and can explain many features commonly observed immediately after concussion. We propose that some acute symptoms of concussion in vertebrates can be explained by the opening of GIRKs following mechanosensory stimulation to the head.
[Mh] Termos MeSH primário: Acetilcolina/metabolismo
Canais de Potássio Corretores do Fluxo de Internalização Acoplados a Proteínas G/metabolismo
Neurônios/metabolismo
Receptores Muscarínicos/metabolismo
[Mh] Termos MeSH secundário: Animais
Concussão Encefálica/metabolismo
Tronco Encefálico/metabolismo
Seres Humanos
Oócitos/metabolismo
Vertebrados/metabolismo
Xenopus laevis/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (G Protein-Coupled Inwardly-Rectifying Potassium Channels); 0 (Receptors, Muscarinic); N9YNS0M02X (Acetylcholine)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE


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[PMID]:29305262
[Au] Autor:Ågren R; Sahlholm K; Nilsson J; Århem P
[Ad] Endereço:Department of Neuroscience, Retzius väg 8, Karolinska Institutet, SE-171 77, Stockholm, Sweden. Electronic address: richard.agren@stud.ki.se.
[Ti] Título:Point mutation of a conserved aspartate, D69, in the muscarinic M receptor does not modify voltage-sensitive agonist potency.
[So] Source:Biochem Biophys Res Commun;496(1):101-104, 2018 01 29.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The muscarinic M receptor (M R) has been shown to display voltage-sensitive agonist binding, based on G protein-activated inward rectifier potassium channel (GIRK) opening and radioligand binding at different membrane voltages. A conserved aspartate in transmembrane segment (TM) II of M R, D69, has been proposed as the voltage sensor. While a recent paper instead presented evidence of tyrosines in TMs III, VI, and VII acting as voltage sensors, these authors were not able to record GIRK channel activation by a D69N mutant M R. In the present study, we succeeded in recording ACh-induced GIRK channel activation by this mutant at -80 and 0 mV. The acetylcholine EC was about 2.5-fold higher at 0 mV, a potency shift very similar to that observed at wild-type M R, indicating that voltage sensitivity persists at the D69N mutant. Thus, our present observations corroborate the notion that D69 is not responsible for voltage sensitivity of the M R.
[Mh] Termos MeSH primário: Acetilcolina/administração & dosagem
Canais de Potássio Corretores do Fluxo de Internalização Acoplados a Proteínas G/metabolismo
Potenciais da Membrana/efeitos dos fármacos
Potenciais da Membrana/fisiologia
Receptor Muscarínico M2/genética
Receptor Muscarínico M2/metabolismo
[Mh] Termos MeSH secundário: Animais
Ácido Aspártico/genética
Células Cultivadas
Sequência Conservada
Relação Dose-Resposta a Droga
Mutagênese Sítio-Dirigida
Oócitos
Mutação Puntual/genética
Receptor Muscarínico M2/efeitos dos fármacos
Relação Estrutura-Atividade
Xenopus laevis
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (G Protein-Coupled Inwardly-Rectifying Potassium Channels); 0 (Receptor, Muscarinic M2); 30KYC7MIAI (Aspartic Acid); N9YNS0M02X (Acetylcholine)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180215
[Lr] Data última revisão:
180215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180107
[St] Status:MEDLINE


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[PMID]:29260547
[Au] Autor:Hong SM; Soe KH; Lee TH; Kim IS; Lee YM; Lim BO
[Ad] Endereço:BK21PLUS Glocal Education Program of Nutraceuticals Development, Konkuk University , Chungju-si, Chungcheongbuk-do 27478, Republic of Korea.
[Ti] Título:Cognitive Improving Effects by Highbush Blueberry (Vaccinium crymbosum L.) Vinegar on Scopolamine-Induced Amnesia Mice Model.
[So] Source:J Agric Food Chem;66(1):99-107, 2018 Jan 10.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The present study aimed to evaluate the preventive effects of highbush blueberry (Vaccinium corymbosum L.) vinegar (BV) on cognitive functions in a scopolamine (Sco)-induced amnesia model in mice. In this study, Sco (1 mg/kg, intraperitoneal injection) was used to induce amnesia. ICR mice were orally administered donepezil (5 mg/kg), blueberry extract (120 mg/kg), and BV (120 mg/kg) for 7 days. After inducing cognitive impairment by Sco, a behavioral assessment using behavior tests (i.e., Y-maze and passive avoidance tests) was performed. The BV group showed significantly restored cognitive function in the behavioral tests. BV facilitated cholinergic activity by inhibiting acetylcholinesterase activity, and enhanced antioxidant enzyme activity. Furthermore, BV was found to be rehabilitated in the cornu ammonis 1 neurons of hippocampus. In our study, we demonstrated that the memory protection conferred by BV was linked to activation of brain-derived neurotrophic factor (BDNF)/cAMP response element binding protein (CREB)/serine-threonine kinase (AKT) signaling.
[Mh] Termos MeSH primário: Amnésia/tratamento farmacológico
Mirtilos Azuis (Planta)/química
Cognição/efeitos dos fármacos
Fármacos Neuroprotetores/farmacologia
Extratos Vegetais/farmacologia
[Mh] Termos MeSH secundário: Acetilcolina/metabolismo
Acetilcolinesterase/metabolismo
Amnésia/induzido quimicamente
Animais
Aprendizagem da Esquiva/efeitos dos fármacos
Córtex Cerebral/efeitos dos fármacos
Córtex Cerebral/metabolismo
Modelos Animais de Doenças
Hipocampo/efeitos dos fármacos
Hipocampo/metabolismo
Aprendizagem em Labirinto
Camundongos Endogâmicos ICR
Extratos Vegetais/química
Hidrobrometo de Escopolamina/toxicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Neuroprotective Agents); 0 (Plant Extracts); 451IFR0GXB (Scopolamine Hydrobromide); EC 3.1.1.7 (Acetylcholinesterase); N9YNS0M02X (Acetylcholine)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180215
[Lr] Data última revisão:
180215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171221
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b03965


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[PMID]:29287782
[Au] Autor:Liaquat L; Batool Z; Sadir S; Rafiq S; Shahzad S; Perveen T; Haider S
[Ad] Endereço:Neurochemistry and Biochemical Neuropharmacology Research Unit, Department of Biochemistry, University of Karachi, Karachi 75270, Pakistan.
[Ti] Título:Naringenin-induced enhanced antioxidant defence system meliorates cholinergic neurotransmission and consolidates memory in male rats.
[So] Source:Life Sci;194:213-223, 2018 Feb 01.
[Is] ISSN:1879-0631
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:AIMS: Free radical mediated neurotoxicity is a leading cause of neurodegenerative disorders. Neurodegeneration due to oxidative stress can produce cognitive dysfunctions. Flavonoids and curcuminoids are naturally occurring polyphenolic compounds that display a variety of therapeutic importance against oxidative stress. MAIN METHODS: This study was designed to assess potential role of polyphenolic compounds in neurocognitive functions and prevention against oxidative stress. For this purpose, young rats were orally treated with naringenin (NAR), curcumin (CUR) and quercetin (QUE) at a dose of 50mg/kg, 200mg/kg and 50mg/kg respectively for 16days. At 4th day of drug administration cognitive functions were monitored by Morris water maze (MWM) test. In MWM cognitive functions in terms of learning acquisition (1h after training), retention (24h after training), memory extinction (4days after training), and reconsolidation (8 and 12days after training) were monitored. Biochemical and neurochemical analysis were done in whole brain. KEY FINDINGS: Treatment of NAR, CUR and QUE significantly enhanced learning acquisition, memory retention and reconsolidation and prevented memory extinction. Treatment of NAR and QUE prevented the alteration of brain antioxidant defence system by enhancing antioxidant enzyme activities and increasing antioxidant compound concentration. Oxidative stress in terms of lipid peroxidation was significantly prevented in treated rats. Serotonergic and cholinergic improvement was also found in treated rats. SIGNIFICANCE: The present study therefore provides biological evidence supporting the usefulness of these polyphenolic compounds in daily life for improvement of cognitive abilities and hence may have a potential role in the management of dementia and related disorders.
[Mh] Termos MeSH primário: Antioxidantes/farmacologia
Cognição/efeitos dos fármacos
Curcumina/farmacologia
Flavanonas/farmacologia
Memória/efeitos dos fármacos
Estresse Oxidativo/efeitos dos fármacos
Quercetina/farmacologia
[Mh] Termos MeSH secundário: Acetilcolina/metabolismo
Acetilcolinesterase/metabolismo
Animais
Antioxidantes/administração & dosagem
Encéfalo/efeitos dos fármacos
Encéfalo/metabolismo
Colinérgicos/farmacologia
Curcumina/administração & dosagem
Flavanonas/administração & dosagem
Masculino
Quercetina/administração & dosagem
Ratos
Ratos Wistar
Serotonina/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Cholinergic Agents); 0 (Flavanones); 333DO1RDJY (Serotonin); 9IKM0I5T1E (Quercetin); EC 3.1.1.7 (Acetylcholinesterase); HN5425SBF2 (naringenin); IT942ZTH98 (Curcumin); N9YNS0M02X (Acetylcholine)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180213
[Lr] Data última revisão:
180213
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171231
[St] Status:MEDLINE


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[PMID]:28743514
[Au] Autor:Herbert J; Thiermann H; Worek F; Wille T
[Ad] Endereço:Bundeswehr Institute of Pharmacology and Toxicology, Neuherbergstrasse 11, 80937 Munich, Germany.
[Ti] Título:Precision cut lung slices as test system for candidate therapeutics in organophosphate poisoning.
[So] Source:Toxicology;389:94-100, 2017 08 15.
[Is] ISSN:1879-3185
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:Standard therapeutic options in organophosphate (OP) poisoning are limited to the administration of atropine and oximes, a regimen often lacking in efficacy and applicability. Treatment alternatives are needed, preferably covering a broad spectrum of OP intoxications. Although recent research yielded several promising compounds, e.g. bioscavengers, modulators of the muscarinic acetylcholine (ACh) receptor or bispyridinium non-oximes, these substances still need further evaluation, especially regarding effects on the potentially lethal respiratory symptoms of OP poisoning. Aim of this study was the development of an applicable and easy method to test the therapeutic efficiency of such substances. For this purpose, airway responsiveness in viable precision cut lung slices (PCLS) from rats was analysed. We showed that ACh-induced airway contractions were spontaneously reversible in non-poisoned PCLS, whereas in OP poisoned PCLS, contractions were irreversible. This effect could be antagonized by addition of the standard therapeutic atropine, thereby presenting a clear indication for treatment efficiency. Now, candidate therapeutic compounds can be evaluated, based on their ability to counteract the irreversible airway contraction in OP poisoned PCLS.
[Mh] Termos MeSH primário: Antídotos/farmacologia
Atropina/farmacologia
Broncoconstrição/efeitos dos fármacos
Broncodilatadores/farmacologia
Descoberta de Drogas/métodos
Pulmão/efeitos dos fármacos
Antagonistas Muscarínicos/farmacologia
Contração Muscular/efeitos dos fármacos
Músculo Liso/efeitos dos fármacos
Agentes Neurotóxicos/toxicidade
Intoxicação por Organofosfatos/tratamento farmacológico
Organofosfatos/toxicidade
[Mh] Termos MeSH secundário: Acetilcolina/farmacologia
Animais
Relação Dose-Resposta a Droga
Pulmão/fisiopatologia
Masculino
Músculo Liso/fisiopatologia
Intoxicação por Organofosfatos/fisiopatologia
Ratos Wistar
Fatores de Tempo
Técnicas de Cultura de Tecidos
Sobrevivência de Tecidos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antidotes); 0 (Bronchodilator Agents); 0 (Muscarinic Antagonists); 0 (Nerve Agents); 0 (Organophosphates); 7C0697DR9I (Atropine); N9YNS0M02X (Acetylcholine)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:180122
[Lr] Data última revisão:
180122
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170727
[St] Status:MEDLINE


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[PMID]:29283235
[Au] Autor:Silkis IG; Makechiv VA
[Ti] Título:Possible Mechanisms of Influence of Various Concentrations of Acetylcholine on Hippocampal Functioning.
[So] Source:Usp Fiziol Nauk;47(4):57-75, 2016 Oct-Dec.
[Is] ISSN:0301-1798
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:Analysis of features of influence of acetylcholine on the hippocampal functioning was performed basing on the modulation rules for the efficacy of excitatory and inhibitory synaptic transmission we earlier proposed, and also on the known data about location of pre- and postsynaptic muscarine and nicotinic receptors. According to these rules, activation of postsynaptic muscarine М1/М3 and nicotinic receptors should promote long-term potentiation of excitatory and depressions (LTD) of inhibitory input to a neuron, whereas action on М2/М4 receptors should promote LTD of excitatory input and a decrease in neuromodulator release. If inhibitory input is stronger than excitatory, LTP (LTD) of excitatory input to the interneuron should promote LTD (LTP) of excitatory input to a target cell. It follows from the proposed mechanism that a lowing concentration of acetylcholine in the hippocampus, a decrease in density of М1/ М3 and a4p2 receptors, and augmenting binding of М2 receptors must lead to a depression of responses of pyramidal neurons in СА3 and СА1 fields to signals from the entorhinal cortex. Thereof, interaction of the semantic information, stored in the cortex, with the information of an episode-, stored in the hippocampus must be hindered and this effect can underlie disturbances of recall of stored information at Alzheimer's disease.
[Mh] Termos MeSH primário: Acetilcolina/farmacologia
Doença de Alzheimer/metabolismo
Agonistas Colinérgicos/farmacologia
Hipocampo/efeitos dos fármacos
Potenciação de Longa Duração/efeitos dos fármacos
Depressão Sináptica de Longo Prazo/efeitos dos fármacos
[Mh] Termos MeSH secundário: Acetilcolina/metabolismo
Doença de Alzheimer/genética
Doença de Alzheimer/fisiopatologia
Animais
Agonistas Colinérgicos/metabolismo
Córtex Entorrinal/efeitos dos fármacos
Córtex Entorrinal/fisiologia
Regulação da Expressão Gênica
Hipocampo/fisiologia
Seres Humanos
Potenciação de Longa Duração/fisiologia
Depressão Sináptica de Longo Prazo/fisiologia
Neurônios/citologia
Neurônios/efeitos dos fármacos
Neurônios/metabolismo
Receptores Muscarínicos/genética
Receptores Muscarínicos/metabolismo
Receptores Nicotínicos/genética
Receptores Nicotínicos/metabolismo
Sinapses/efeitos dos fármacos
Sinapses/fisiologia
Transmissão Sináptica/efeitos dos fármacos
Transmissão Sináptica/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Cholinergic Agonists); 0 (Receptors, Muscarinic); 0 (Receptors, Nicotinic); N9YNS0M02X (Acetylcholine)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180116
[Lr] Data última revisão:
180116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171229
[St] Status:MEDLINE


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[PMID]:29175450
[Au] Autor:Albano GD; Bonanno A; Moscato M; Anzalone G; Di Sano C; Riccobono L; Wenzel SE; Profita M
[Ad] Endereço:Institute of Biomedicine and Molecular Immunology "A. Monroy" (IBIM), National Research Council of Italy (CNR), Palermo, Italy.
[Ti] Título:Crosstalk between mAChRM3 and ß2AR, via acetylcholine PI3/PKC/PBEP1/Raf-1 MEK1/2/ERK1/2 pathway activation, in human bronchial epithelial cells after long-term cigarette smoke exposure.
[So] Source:Life Sci;192:99-109, 2018 Jan 01.
[Is] ISSN:1879-0631
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Cigarette smoke extract (CSE) affects the expression of non-neuronal components of cholinergic system in bronchial epithelial cells and, as PEBP1/Raf-mediated MAPK1/2 and ERK1/2 pathway, promotes inflammation and oxidative stress. AIMS: We studied whether Acetylcholine (ACh) is involved in the mechanism of crosstalk between mAChRM3 and ß2Adrenergic receptors (ß2AR) promoting, via PI3/PKC/PBEP1/Raf/MEK1/2/ERK1/2 activation, ß2AR desensitization, inflammation and, oxidative stress in a bronchial epithelial cell line (16HBE) after long-term exposure to cigarette smoke extract (LECSE). METHODS: We evaluated mAChRM3 and Choline Acetyltransferase (ChAT) expression, ACh production, PEBP1, ERk1/2, and ß2AR phosphorylation, as well as NOX-4, ROS production and IL-8 release in 16HBE after LECSE. The inhibitory activity of Hemicholinium (HCh-3) (a potent choline uptake blocker), LY294002 (a highly selective inhibitor of PI3 kinase), Tiotropium (Spiriva®) (anticholinergic drug) and Olodaterol (ß AR agonist), were tested in 16HBE after LECSE. RESULTS: mAChRM3, ChAT, ACh activity, pPEBP1, pß2AR, pERK1/2, ROS, NOX-4 and IL-8 increased after LECSE in 16HBE LECSE compared to untreated cells. HCh-3 and LY294002 (alone or in combination) as well as Tiotropium (Spiriva®) or Olodaterol (alone or in combination) all reduced the levels of pPEBP1, pß2AR, pERK1/2, ROS, NOX-4, and IL-8 in 16HBE LECSE compared to untreated cells. CONCLUSIONS: LECSE promotes ACh production which enhances PI3/PKC/PEBP1/Raf-ERK1/2 pathway activation, heterologous ß2AR desensitization, as well as release of inflammatory and oxidative mediators in bronchial epithelial cells. The use of anticholinergic drugs and long-acting ß2-agonists, alone or in combination may be dampen these inflammatory mechanisms when used in combination in some epithelial cell types.
[Mh] Termos MeSH primário: Acetilcolina/metabolismo
Brônquios/patologia
Células Epiteliais/efeitos dos fármacos
Quinase 2 de Receptor Acoplado a Proteína G
Receptor Cross-Talk/efeitos dos fármacos
Receptores Adrenérgicos beta 2
Transdução de Sinais/efeitos dos fármacos
Fumaça/efeitos adversos
Fumar/patologia
Tabaco/química
[Mh] Termos MeSH secundário: Brônquios/citologia
Brônquios/efeitos dos fármacos
Citocinas/biossíntese
Seres Humanos
MAP Quinase Quinase Quinases/antagonistas & inibidores
Sistema de Sinalização das MAP Quinases/efeitos dos fármacos
Fosfatidilinositol 3-Quinases/antagonistas & inibidores
Fosfatidilinositol 3-Quinases/metabolismo
Proteína Quinase C/antagonistas & inibidores
Proteína Quinase C/metabolismo
Inibidores de Proteínas Quinases/farmacologia
Quinases raf/antagonistas & inibidores
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (ADRB2 protein, human); 0 (Cytokines); 0 (Protein Kinase Inhibitors); 0 (Receptors, Adrenergic, beta-2); 0 (Smoke); EC 2.7.1.- (Phosphatidylinositol 3-Kinases); EC 2.7.11.1 (raf Kinases); EC 2.7.11.13 (Protein Kinase C); EC 2.7.11.15 (muscarinic receptor kinase); EC 2.7.11.16 (G-Protein-Coupled Receptor Kinase 2); EC 2.7.11.25 (MAP Kinase Kinase Kinases); N9YNS0M02X (Acetylcholine)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180108
[Lr] Data última revisão:
180108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171128
[St] Status:MEDLINE


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[PMID]:28458421
[Au] Autor:Saikia B; Barua CC; Haloi P; Patowary P
[Ad] Endereço:Department of Pharmacology and Toxicology, College of Veterinary Science, Assam Agricultural University, Guwahati, Assam, India.
[Ti] Título:Anticholinergic, antihistaminic, and antiserotonergic activity of n-hexane extract of seeds on isolated tissue preparations: An study.
[So] Source:Indian J Pharmacol;49(1):42-48, 2017 Jan-Feb.
[Is] ISSN:1998-3751
[Cp] País de publicação:India
[La] Idioma:eng
[Ab] Resumo:OBJECTIVES: The aim of this study was to evaluate anticholinergic, antihistaminic, and antiserotonergic activity of the n-hexane extract of the seeds of (ZAHE) on isolated ileum of rat and guinea pig and fundus of rat. MATERIALS AND METHODS: ZAHE was prepared using soxhlet extraction and cumulative concentration response curves were constructed using various doses on the tissues for acetylcholine (ACh), 5-hydroxytryptamine (5-HT), and histamine with or without n-hexane extract. Atropine, ketanserin, and pheniramine maleate were used as antagonists for ACh, serotonin, and histamine, respectively. RESULTS: ZAHE-induced concentration-dependent inhibition of isolated ileum and fundus in rat and ileum of guinea pig. The half maximal effective concentration (EC ) of ACh in the presence of atropine (10 M; < 0.05) and ZAHE (1000 µg/ml; < 0.01) was significantly higher than EC of ACh alone. The EC of 5-HT in the presence of ketanserin (10 M; < 0.01) and ZAHE (1000 µg/ml; < 0.05) was higher than EC of 5-HT alone. Similarly, the EC of histamine in the presence of pheniramine maleate (10 M; < 0.01) and ZAHE (300 µg/ml; < 0.01 and 1000 µg/ml; < 0.05) was also significantly higher than EC of histamine alone. CONCLUSION: From the study, it was observed that ZAHE shows significant anticholinergic, antiserotonergic, and antihistaminic activity. The study provides sufficient evidence that the seeds can be used in gastric disorders, cough, chest infection, etc., as per folklore claims.
[Mh] Termos MeSH primário: Antagonistas Colinérgicos/farmacologia
Antagonistas dos Receptores Histamínicos/farmacologia
Extratos Vegetais/farmacologia
Antagonistas da Serotonina/farmacologia
Zanthoxylum/química
[Mh] Termos MeSH secundário: Acetilcolina/metabolismo
Animais
Antagonistas Colinérgicos/administração & dosagem
Antagonistas Colinérgicos/isolamento & purificação
Relação Dose-Resposta a Droga
Fundo Gástrico/efeitos dos fármacos
Fundo Gástrico/metabolismo
Cobaias
Hexanos/química
Histamina/metabolismo
Antagonistas dos Receptores Histamínicos/administração & dosagem
Antagonistas dos Receptores Histamínicos/isolamento & purificação
Íleo/efeitos dos fármacos
Íleo/metabolismo
Masculino
Extratos Vegetais/administração & dosagem
Ratos
Ratos Wistar
Sementes
Serotonina/metabolismo
Antagonistas da Serotonina/administração & dosagem
Antagonistas da Serotonina/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cholinergic Antagonists); 0 (Hexanes); 0 (Histamine Antagonists); 0 (Plant Extracts); 0 (Serotonin Antagonists); 2DDG612ED8 (n-hexane); 333DO1RDJY (Serotonin); 820484N8I3 (Histamine); N9YNS0M02X (Acetylcholine)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171229
[Lr] Data última revisão:
171229
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170502
[St] Status:MEDLINE
[do] DOI:10.4103/0253-7613.201025


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[PMID]:29096805
[Au] Autor:Aziz A; Hansen HS; Sechtem U; Prescott E; Ong P
[Ad] Endereço:Department of Cardiology, Robert Bosch Krankenhaus, Stuttgart, Germany; Department of Cardiology, Odense University Hospital, Odense, Denmark. Electronic address: Ahmed.Aziz@rsyd.dk.
[Ti] Título:Sex-Related Differences in Vasomotor Function in Patients With Angina and Unobstructed Coronary Arteries.
[So] Source:J Am Coll Cardiol;70(19):2349-2358, 2017 Nov 07.
[Is] ISSN:1558-3597
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Coronary vasomotor dysfunction is an important mechanism for angina in patients with unobstructed coronary arteries. OBJECTIVES: The purpose of this study was to determine sex differences in the prevalence and clinical presentation of vasomotor dysfunction in a European population and to examine sex differences in the dose of acetylcholine leading to a positive acetylcholine provocation test (ACH test). METHODS: Between 2007 and 2014, we included 1,379 consecutive patients with stable angina, unobstructed coronaries and ACH test performed for epicardial vasospasm or coronary microvascular dysfunction (CMD) due to microvascular spasm. The predictive value of sex, risk factors, symptoms, and noninvasive test results was analyzed by means of logistic regression. RESULTS: The mean patient age was 62 years, and 42% were male. There were 813 patients (59%) with a pathological ACH test, 33% for CMD and 26% for epicardial vasospasm. A pathological test was more common in females (70% vs. 43%; p < 0.001). In a multivariable logistic regression model the sex difference was statistically significant with a female-male odds ratio for CMD and epicardial vasospasm of 4.2 (95% confidence interval: 3.1 to 5.5; p < 0.001) and 2.3 (95% confidence interval: 1.7 to 3.1; p < 0.001), respectively. Effort-related symptoms, but neither risk factors nor noninvasive stress tests, contributed to predicting a pathological test. Female patients were more sensitive to acetylcholine with vasomotor dysfunction occurring at lower ACH doses compared with male patients. CONCLUSIONS: Vasomotor dysfunction is frequent in patients with angina and unobstructed coronaries in a European population. Female patients have a higher prevalence of vasomotor dysfunction (especially CMD) compared with male patients. A pathological ACH test was observed at lower ACH doses in women compared with men.
[Mh] Termos MeSH primário: Angina Pectoris/fisiopatologia
Vasoespasmo Coronário/fisiopatologia
Vasos Coronários/fisiologia
Caracteres Sexuais
Vasodilatadores/farmacologia
Sistema Vasomotor/fisiologia
[Mh] Termos MeSH secundário: Acetilcolina/farmacologia
Idoso
Angina Pectoris/epidemiologia
Vasoespasmo Coronário/induzido quimicamente
Vasoespasmo Coronário/epidemiologia
Vasos Coronários/efeitos dos fármacos
Relação Dose-Resposta a Droga
Europa (Continente)/epidemiologia
Feminino
Seres Humanos
Masculino
Meia-Idade
Estudos Retrospectivos
Sistema Vasomotor/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Vasodilator Agents); N9YNS0M02X (Acetylcholine)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171109
[Lr] Data última revisão:
171109
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:171104
[St] Status:MEDLINE



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