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[Au] Autor:Galkin OY; Besarab AB; Lutsenko TN
[Ti] Título:Characteristics of enzyme-linked immunosorbent assay for detection of IgG antibodies specific to Сhlamydia trachomatis heat shock protein (HSP-60)
[So] Source:Ukr Biochem J;89(1):22-30, 2017 Jan-Feb.
[Is] ISSN:2409-4943
[Cp] País de publicação:Ukraine
[La] Idioma:eng
[Ab] Resumo:The goal of this work was to study sensitivity and specificity of the developed ELISA set for the identification of IgG antibodies against Chlamydia trachomatis HSP-60 (using biotinylated tyramine-based signal amplification system). The study was conducted using a panel of characterized sera, as well as two reference ELISA sets of similar purpose. According to the results of ELISA informative value parameters, the ELISA we have developed showed the highest specificity and sensitivity parameters (no false negative or false positive results were registered). In 4 out of 15 intralaboratory panel serum samples initially identified as negative, anti-HSP-60 IgG-antibodies test result in reference ELISA sets upon dilution changed from negative to positive. The nature of titration curves of false negative sera and commercial monoclonal antibodies А57-В9 against C. trachomatis HSP-60 after incubation for 24 h was indicative of the presence of anti-idiotypic antibodies in these samples. Upon sera dilution, idiotypic-anti-idiotypic complexes dissociated, which caused the change of test result. High informative value of the developed ELISA set for identification of IgG antibodies against C. trachomatis HSP-60 has been proven. Anti-idiotypic antibodies possessing C. trachomatis anti-HSP-60 activity and being one of the causes of false negative results of the relevant ELISA-based tests have been identified in blood sera of individuals infected with chlamydial genitourinary infection agents.
[Mh] Termos MeSH primário: Anticorpos Antibacterianos/análise
Antígenos de Bactérias/sangue
Chaperonina 60/sangue
Infecções por Chlamydia/diagnóstico
Chlamydia trachomatis/imunologia
Ensaio de Imunoadsorção Enzimática/métodos
Imunoglobulina G/análise
[Mh] Termos MeSH secundário: Anticorpos Anti-Idiotípicos/química
Anticorpos Antibacterianos/sangue
Anticorpos Antibacterianos/imunologia
Complexo Antígeno-Anticorpo/química
Antígenos de Bactérias/imunologia
Chaperonina 60/imunologia
Infecções por Chlamydia/sangue
Infecções por Chlamydia/imunologia
Infecções por Chlamydia/microbiologia
Chlamydia trachomatis/química
Ensaio de Imunoadsorção Enzimática/normas
Reações Falso-Negativas
Seres Humanos
Soros Imunes/química
Imunoglobulina G/sangue
Imunoglobulina G/imunologia
Sensibilidade e Especificidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Anti-Idiotypic); 0 (Antibodies, Bacterial); 0 (Antigen-Antibody Complex); 0 (Antigens, Bacterial); 0 (Chaperonin 60); 0 (Immune Sera); 0 (Immunoglobulin G); X8ZC7V0OX3 (Tyramine)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180116
[Lr] Data última revisão:
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171214
[St] Status:MEDLINE
[do] DOI:10.15407/ubj89.01.022

  2 / 4901 MEDLINE  
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[Au] Autor:Li Y; Lucas-Osma AM; Black S; Bandet MV; Stephens MJ; Vavrek R; Sanelli L; Fenrich KK; Di Narzo AF; Dracheva S; Winship IR; Fouad K; Bennett DJ
[Ad] Endereço:Neuroscience and Mental Health Institute and Faculty of Rehabilitation Medicine, University of Alberta, Edmonton, Alberta, Canada.
[Ti] Título:Pericytes impair capillary blood flow and motor function after chronic spinal cord injury.
[So] Source:Nat Med;23(6):733-741, 2017 Jun.
[Is] ISSN:1546-170X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Blood vessels in the central nervous system (CNS) are controlled by neuronal activity. For example, widespread vessel constriction (vessel tone) is induced by brainstem neurons that release the monoamines serotonin and noradrenaline, and local vessel dilation is induced by glutamatergic neuron activity. Here we examined how vessel tone adapts to the loss of neuron-derived monoamines after spinal cord injury (SCI) in rats. We find that, months after the imposition of SCI, the spinal cord below the site of injury is in a chronic state of hypoxia owing to paradoxical excess activity of monoamine receptors (5-HT ) on pericytes, despite the absence of monoamines. This monoamine-receptor activity causes pericytes to locally constrict capillaries, which reduces blood flow to ischemic levels. Receptor activation in the absence of monoamines results from the production of trace amines (such as tryptamine) by pericytes that ectopically express the enzyme aromatic L-amino acid decarboxylase (AADC), which synthesizes trace amines directly from dietary amino acids (such as tryptophan). Inhibition of monoamine receptors or of AADC, or even an increase in inhaled oxygen, produces substantial relief from hypoxia and improves motoneuron and locomotor function after SCI.
[Mh] Termos MeSH primário: Monoaminas Biogênicas/metabolismo
Traumatismos da Medula Espinal/metabolismo
[Mh] Termos MeSH secundário: Animais
Descarboxilases de Aminoácido-L-Aromático/metabolismo
Capilares/efeitos dos fármacos
Injeções Espinhais
Locomoção/efeitos dos fármacos
Microscopia Confocal
Microscopia de Interferência
RNA Mensageiro/metabolismo
Receptor 5-HT1B de Serotonina/metabolismo
Receptores Adrenérgicos alfa 2/metabolismo
Receptores 5-HT1 de Serotonina/metabolismo
Antagonistas do Receptor 5-HT1 de Serotonina/farmacologia
Traumatismos da Medula Espinal/patologia
Traumatismos da Medula Espinal/fisiopatologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biogenic Monoamines); 0 (RNA, Messenger); 0 (Receptor, Serotonin, 5-HT1B); 0 (Receptors, Adrenergic, alpha-2); 0 (Receptors, Serotonin, 5-HT1); 0 (Serotonin 5-HT1 Receptor Antagonists); 0 (Tryptamines); 333DO1RDJY (Serotonin); 422ZU9N5TV (tryptamine); EC (Aromatic-L-Amino-Acid Decarboxylases); S88TT14065 (Oxygen); X4W3ENH1CV (Norepinephrine); X8ZC7V0OX3 (Tyramine)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171206
[Lr] Data última revisão:
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170502
[St] Status:MEDLINE
[do] DOI:10.1038/nm.4331

  3 / 4901 MEDLINE  
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[Au] Autor:Jiang W; Chen Y; He X; Hu S; Li S; Liu Y
[Ad] Endereço:Laboratory of Seafood Processing, Innovative and Application Institute, Zhejiang Ocean University, Zhoushan, Zhejiang 316022, China.
[Ti] Título:A study of the tyramine/glucose Maillard reaction: Variables, characterization, cytotoxicity and preliminary application.
[So] Source:Food Chem;239:377-384, 2018 Jan 15.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The tyramine/glucose Maillard reaction was proposed as an emerging tool for tyramine reduction in a model system and two commercial soy sauce samples. The model system was composed of tyramine and glucose in buffer solutions with or without NaCl. The results showed that tyramine was reduced in the model system, and the reduction rate was affected by temperature, heating time, initial pH value, NaCl concentration, initial glucose concentration and initial tyramine concentration. Changes in fluorescence intensity and ultraviolet-visible (UV-vis) absorption spectra showed three stages of the Maillard reaction between tyramine and glucose. Cytotoxicity assay demonstrated that tyramine/glucose Maillard reaction products (MRPs) were significantly less toxic than that of tyramine (p<0.05). Moreover, tyramine concentration in soy sauce samples was significantly reduced when heated with the addition of glucose (p<0.05). Experimental results showed that the tyramine/glucose Maillard reaction is a promising method for tyramine reduction in foods.
[Mh] Termos MeSH primário: Glucose/química
[Mh] Termos MeSH secundário: Reação de Maillard
Alimentos de Soja
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
IY9XDZ35W2 (Glucose); X8ZC7V0OX3 (Tyramine)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170907
[St] Status:MEDLINE

  4 / 4901 MEDLINE  
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[Au] Autor:Callejón S; Sendra R; Ferrer S; Pardo I
[Ad] Endereço:ENOLAB-Estructura de Recerca Interdisciplinar BioTecMed and Departament de Microbiologia i Ecologia. Universitat de València, c/ Dr. Moliner 50, Burjassot, Spain.
[Ti] Título:Recombinant laccase from Pediococcus acidilactici CECT 5930 with ability to degrade tyramine.
[So] Source:PLoS One;12(10):e0186019, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Biogenic amines degradation by bacterial laccases is little known, so we have cloned and heterologously expressed, in E. coli, a new laccase from Pediococcus acidilactici CECT 5930 (Lpa5930), a lactic acid bacterium commonly found in foods able to degrade tyramine. The recombinant enzyme has been characterized by physical and biochemical assays. Here we report the optimization of expression and purification procedures of this laccase. DNA encoding sequence of laccase from P. acidilactici was amplified by PCR and cloned into the expression plasmid pET28a for induction by isopropyl-ß-D-thiogalactoipyranoside. Protein expression was performed in E. coli BL21(DE3) harboring pGro7 plasmid expressing a chaperone folding assistant induced by arabinose. Purification was performed by column metal-chelating chromatography on Ni-NTA-agarose. The laccase enzyme obtained has an apparent molecular mass of ∼60 kDa, an optimum temperature activity toward 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) of 28°C, and was quickly inactivated at temperatures higher than 70°C. The apparent Km value for ABTS was 1.7 mM and the Vmax obtained was 24 U/mg. In addition to ABTS, recombinant Lpa5930 laccase degraded the biogenic amine tyramine at pH 9.5 and pH 4.0 with or without ABTS as a mediator. Tyramine degradation by laccases could solve the problems generated in food due to the presence of this toxic compound.
[Mh] Termos MeSH primário: Lacase/metabolismo
Pediococcus acidilactici/enzimologia
Proteínas Recombinantes/isolamento & purificação
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Proteínas de Bactérias/química
Sequência de Bases
Clonagem Molecular
Eletroforese em Gel de Poliacrilamida
Concentração de Íons de Hidrogênio
Proteínas Recombinantes/metabolismo
Análise de Sequência de DNA
Espectrofotometria Ultravioleta
Especificidade por Substrato
Ácidos Sulfônicos/metabolismo
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Benzothiazoles); 0 (Recombinant Proteins); 0 (Sulfonic Acids); 28752-68-3 (2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid); EC (Laccase); X8ZC7V0OX3 (Tyramine)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171012
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0186019

  5 / 4901 MEDLINE  
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[Au] Autor:Einarson OJ; Sen D
[Ad] Endereço:Department of Chemistry, Simon Fraser University, Burnaby, British Columbia V5A 1S6, Canada.
[Ti] Título:Self-biotinylation of DNA G-quadruplexes via intrinsic peroxidase activity.
[So] Source:Nucleic Acids Res;45(17):9813-9822, 2017 Sep 29.
[Is] ISSN:1362-4962
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The striking and ubiquitous in vitro affinity between hemin and DNA/RNA G-quadruplexes raises the intriguing possibility of its relevance to biology. To date, no satisfactory experimental framework has been reported for investigating such a possibility. Complexation by G-quadruplexes leads to activation of the bound hemin toward catalysis of 1- and 2-electron oxidative reactions, with phenolic compounds being particularly outstanding substrates. We report here a strategy for exploiting that intrinsic peroxidase activity of hemin•G-quadruplex complexes for self-biotinylation of their G-quadruplex component. Such self-biotinylation occurs with good efficiency and high discrimination in vitro, being specific for G-quadruplexes and not for duplexes. The biotinylated DNA, moreover, remains amenable to polymerase chain reaction amplification, rendering it suitable for analysis by ChIP-Seq and related methods. We anticipate that this self-biotinylation methodology will also serve as a sensitive tool, orthogonal to existing ones, for identifying, labeling and pulling down cellular RNA and DNA G-quadruplexes in general, as well as proteins bound to or proximal to such quadruplexes.
[Mh] Termos MeSH primário: DNA Catalítico/química
Quadruplex G
[Mh] Termos MeSH secundário: Biocatálise
Técnicas Biossensoriais/métodos
Peróxido de Hidrogênio/química
Mimetismo Molecular
Reação em Cadeia da Polimerase
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Catalytic); 0 (Oligonucleotides); 0 (Phenols); 0 (biotin tyramine); 6SO6U10H04 (Biotin); 743LRP9S7N (Hemin); 9013-20-1 (Streptavidin); BBX060AN9V (Hydrogen Peroxide); EC 1.11.1.- (Peroxidases); X8ZC7V0OX3 (Tyramine)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171017
[Lr] Data última revisão:
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171004
[St] Status:MEDLINE
[do] DOI:10.1093/nar/gkx765

  6 / 4901 MEDLINE  
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[Au] Autor:Cho H; Kim O; Lee Y; Kang LJ; Nguyen CN; Ishihara A; Kim HE
[Ad] Endereço:Department of Oral Pathology, Dental Science Research Institute and Medical Research Center for Biomineralization Disorders, School of Dentistry, Chonnam National University, 77 Yongbong-ro, Buk-gu, Gwangju, 61186, Republic of Korea.
[Ti] Título:Feruloylserotonin inhibits hydrogen peroxide-induced melanogenesis and apoptosis in B16F10 and SK-Mel-2 melanoma cells.
[So] Source:Biochem Biophys Res Commun;491(4):973-979, 2017 Sep 30.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Feruloylserotonin (FS) is a major bioactive component of safflower seeds, with documented strong antibacterial, anti-inflammatory, and free radical scavenging activities. Reactive oxygen species (ROS) can strongly induce melanogenesis and cell apoptosis. The present study aimed to investigate the ability of FS in preventing hydrogen peroxide (H O )-induced melanogenesis and cell apoptosis. Melanogenesis and apoptotic cell death were induced by transient exposure to H O in B16F10 and SK-Mel-2 melanoma cells. FS significantly inhibited melanogenesis and cell death in both cell lines. FS inhibited H O -induced melanin production by down-regulating CREB/MITF/TYR signaling via inhibited intracellular cAMP accumulation. Additionally, FS induced extracellular regulated kinase activation, which led to the degradation of MITF and consequently decreased TYR expression and melanin production in H O -stimulated cells. Furthermore, FS inhibited H O -induced apoptotic cell death by maintaining mitochondrial membrane potential. Therefore, FS might have potential use for cosmetic whitening and as a therapeutic agent for hyperpigmentation disorder.
[Mh] Termos MeSH primário: Apoptose/efeitos dos fármacos
Ácidos Cumáricos/farmacologia
Peróxido de Hidrogênio/antagonistas & inibidores
Melanoma/tratamento farmacológico
Tiramina/análogos & derivados
[Mh] Termos MeSH secundário: Animais
Linhagem Celular Tumoral
Sobrevivência Celular/efeitos dos fármacos
Relação Dose-Resposta a Droga
Seres Humanos
Peróxido de Hidrogênio/farmacologia
Estrutura Molecular
Relação Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Coumaric Acids); 65646-26-6 (feruloyltyramine); BBX060AN9V (Hydrogen Peroxide); X8ZC7V0OX3 (Tyramine)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171010
[Lr] Data última revisão:
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170803
[St] Status:MEDLINE

  7 / 4901 MEDLINE  
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[Au] Autor:Handa A; Kawanabe H; Ibe A
[Ad] Endereço:Jissen Women's University.
[Ti] Título:Determination of Nonvolatile Amines in Foods by Improved Dansyl Derivatization Reaction.
[So] Source:Shokuhin Eiseigaku Zasshi;58(3):149-154, 2017.
[Is] ISSN:1882-1006
[Cp] País de publicação:Japan
[La] Idioma:jpn
[Ab] Resumo:An analytical method for the determination of nonvolatile amines (putrescine, cadaverine, histamine, tyramine, and spermidine) in foods was developed, using an improved dansyl derivatization technique. The five amines were extracted from food with 1% trichloroacetic acid. Three milliliter of extract was applied to a polymer-based strong cation exchange resin mini-column, which was washed with 5 mL of water, and eluted with 5 mL of 1 mol/L potassium carbonate solution. The eluate was dansylated, then 5 mL of toluene was added with shaking. The toluene layer was evaporated. The residue was taken up in 1 mL of acetonitrile and shaken with 1 mL of 5% proline in 1 mol/L potassium carbonate solution. The upper acetonitrile layer was collected, filtered, and subjected to HPLC. The limits of quantitation for putrescine and cadaverine in the samples were both 0.2 µg/g; those of spermidine, tyramine, and histamine were 0.8, 2.0, and 5.0 µg/g, respectively. The average recoveries of the five amines from nine foods exceeded 80%.
[Mh] Termos MeSH primário: Cromatografia Líquida de Alta Pressão/métodos
Compostos de Dansil
Análise de Alimentos/métodos
[Mh] Termos MeSH secundário: Acetonitrilos
Cadaverina/isolamento & purificação
Histamina/isolamento & purificação
Putrescina/isolamento & purificação
Extração em Fase Sólida/métodos
Espermidina/isolamento & purificação
Ácido Tricloroacético
Tiramina/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acetonitriles); 0 (Dansyl Compounds); 0 (Solutions); 3FPU23BG52 (Toluene); 5V2JDO056X (Trichloroacetic Acid); 820484N8I3 (Histamine); L90BEN6OLL (Cadaverine); QMU9166TJ4 (dansyl chloride); U87FK77H25 (Spermidine); V10TVZ52E4 (Putrescine); X8ZC7V0OX3 (Tyramine); Z072SB282N (acetonitrile)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170711
[St] Status:MEDLINE
[do] DOI:10.3358/shokueishi.58.149

  8 / 4901 MEDLINE  
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[Au] Autor:Khaliullin B; Ayikpoe R; Tuttle M; Latham JA
[Ad] Endereço:From the Department of Chemistry and Biochemistry, University of Denver, Denver, Colorado 80208.
[Ti] Título:Mechanistic elucidation of the mycofactocin-biosynthetic radical -adenosylmethionine protein, MftC.
[So] Source:J Biol Chem;292(31):13022-13033, 2017 Aug 04.
[Is] ISSN:1083-351X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Ribosomally synthesized and posttranslationally modified peptide (RiPP) pathways produce a diverse array of natural products. A subset of these pathways depends on radical -adenosylmethionine proteins to modify the RiPP-produced peptide. Mycofactocin biosynthesis is one example of an -adenosylmethionine protein-dependent RiPP pathway. Recently, it has been shown that MftC catalyzes the oxidative decarboxylation of the C-terminal tyrosine (Tyr-30) on the mycofactocin precursor peptide MftA; however, this product has not been verified by techniques other than MS. Herein, we provide a more detailed study of MftC catalysis and report a revised mechanism for MftC chemistry. We show that MftC catalyzes the formation of two isomeric products. Using a combination of MS, isotope labeling, and H and C NMR techniques, we established that the major product, MftA*, is a tyramine-valine-cross-linked peptide formed by MftC through two -adenosylmethionine-dependent turnovers. In addition, we show that the hydroxyl group on MftA Tyr-30 is required for MftC catalysis. Furthermore, we show that a substitution in the penultimate MftA Val-29 position causes the accumulation of an MftA** minor product. The H NMR spectrum indicates that this minor product contains an αß-unsaturated bond that likely arises from an aborted intermediate of MftA* synthesis. The finding that MftA* is the major product formed during MftC catalysis could have implications for the further elucidation of mycofactocin biosynthesis.
[Mh] Termos MeSH primário: Proteínas de Bactérias/metabolismo
Chaperonas Moleculares/metabolismo
Mycobacterium ulcerans/enzimologia
Precursores de Proteínas/metabolismo
[Mh] Termos MeSH secundário: Substituição de Aminoácidos
Proteínas de Bactérias/química
Proteínas de Bactérias/genética
Cromatografia Líquida de Alta Pressão
Chaperonas Moleculares/química
Chaperonas Moleculares/genética
Mutagênese Sítio-Dirigida
Mycobacterium ulcerans/metabolismo
Ressonância Magnética Nuclear Biomolecular
Domínios e Motivos de Interação entre Proteínas
Precursores de Proteínas/química
Precursores de Proteínas/genética
Proteínas Recombinantes de Fusão/química
Proteínas Recombinantes de Fusão/metabolismo
Espectrometria de Massas por Ionização por Electrospray
Espectrometria de Massas em Tandem
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Molecular Chaperones); 0 (Protein Precursors); 0 (Recombinant Fusion Proteins); 42HK56048U (Tyrosine); 7LP2MPO46S (S-Adenosylmethionine); EC 4.1.1.- (Carboxy-Lyases); HG18B9YRS7 (Valine); X8ZC7V0OX3 (Tyramine)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170825
[Lr] Data última revisão:
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170622
[St] Status:MEDLINE
[do] DOI:10.1074/jbc.M117.795682

  9 / 4901 MEDLINE  
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[Au] Autor:Globisch D; Eubanks LM; Shirey RJ; Pfarr KM; Wanji S; Debrah AY; Hoerauf A; Janda KD
[Ad] Endereço:Department of Chemistry, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, United States; Department of Immunology, The Skaggs Institute for Chemical Biology, The Worm Institute of Research and Medicine (WIRM), The Scripps Research Institute, 10550 North Torrey, La J
[Ti] Título:Validation of onchocerciasis biomarker N-acetyltyramine-O-glucuronide (NATOG).
[So] Source:Bioorg Med Chem Lett;27(15):3436-3440, 2017 08 01.
[Is] ISSN:1464-3405
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The Neglected Tropical Disease onchocerciasis is a parasitic disease. Despite many control programmes by the World Health Organization (WHO), large communities in West and Central Africa are still affected. Besides logistic challenges during biannual mass drug administration, the lack of a robust, point-of-care diagnostic is limiting successful eradication of onchocerciasis. Towards the implementation of a non-invasive and point-of-care diagnostic, we have recently reported the discovery of the biomarker N-acetyltyramine-O-glucuronide (NATOG) in human urine samples using a metabolomics-mining approach. NATOG's biomarker value was enhanced during an investigation in a rodent model. Herein, we further detail the specificity of NATOG in active onchocerciasis infections as well as the co-infecting parasites Loa loa and Mansonella perstans. Our results measured by liquid chromatography coupled with mass spectrometry (LC-MS) reveal elevated NATOG values in mono- and co-infection samples only in the presence of the nematode Onchocerca volvulus. Metabolic pathway investigation of l-tyrosine/tyramine in all investigated nematodes uncovered an important link between the endosymbiotic bacterium Wolbachia and O. volvulus for the biosynthesis of NATOG. Based on these extended studies, we suggest NATOG as a biomarker for tracking active onchocerciasis infections and provide a threshold concentration value of NATOG for future diagnostic tool development.
[Mh] Termos MeSH primário: Glucuronídeos/urina
Espectrometria de Massas/métodos
Doenças Negligenciadas/urina
Onchocerca volvulus/isolamento & purificação
Tiramina/análogos & derivados
[Mh] Termos MeSH secundário: Animais
Cromatografia Líquida/métodos
Seres Humanos
Limite de Detecção
Doenças Negligenciadas/metabolismo
Onchocerca volvulus/metabolismo
[Nm] Nome de substância:
0 (Biomarkers); 0 (Glucuronides); BZB50E9QVY (N-acetyltyramine); X8ZC7V0OX3 (Tyramine)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171125
[Lr] Data última revisão:
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170611
[St] Status:MEDLINE

  10 / 4901 MEDLINE  
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[Au] Autor:Ishihara A; Kumeda R; Hayashi N; Yagi Y; Sakaguchi N; Kokubo Y; Ube N; Tebayashi SI; Ueno K
[Ad] Endereço:a Faculty of Agriculture , Tottori University , Tottori , Japan.
[Ti] Título:Induced accumulation of tyramine, serotonin, and related amines in response to Bipolaris sorokiniana infection in barley.
[So] Source:Biosci Biotechnol Biochem;81(6):1090-1098, 2017 Jun.
[Is] ISSN:1347-6947
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The inducible metabolites were analyzed in barley leaves inoculated with Bipolaris sorokiniana, the causal agent of spot blotch of barley. HPLC analysis revealed that B. sorokiniana-infected leaves accumulated 4 hydrophilic compounds. They were purified by ODS column chromatography and preparative HPLC. Spectroscopic analyses revealed that they were tyramine (1), 3-(2-aminoethyl)-3-hydroxyindolin-2-one (2), serotonin (3), and 5,5'-dihydroxy-2,4'-bitryptamine (4). Among these, 2 and 4 have not been reported as natural products. They showed antifungal activity in an assay of inhibition of B. sorokiniana conidia germination, suggesting that they play a role in the chemical defense of barley as phytoalexins. The accumulation of 1-4 was examined also in the leaves of rice and foxtail millet. Rice leaves accumulated 2, 3, and 4, whereas foxtail millet leaves accumulated 3 and 4 in response to pathogen attack, suggesting the generality of accumulation of 3 and 4 in the Poaceae species.
[Mh] Termos MeSH primário: Antifúngicos/imunologia
Doenças das Plantas/imunologia
Saccharomycetales/efeitos dos fármacos
Esporos Fúngicos/efeitos dos fármacos
[Mh] Termos MeSH secundário: Antifúngicos/metabolismo
Cromatografia Líquida de Alta Pressão
Interações Hidrofóbicas e Hidrofílicas
Doenças das Plantas/microbiologia
Imunidade Vegetal
Folhas de Planta/imunologia
Folhas de Planta/metabolismo
Folhas de Planta/microbiologia
Setaria (Planta)/imunologia
Setaria (Planta)/metabolismo
Setaria (Planta)/microbiologia
Especificidade da Espécie
Esporos Fúngicos/patogenicidade
Esporos Fúngicos/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antifungal Agents); 0 (Indoles); 0 (Sesquiterpenes); 0 (Tryptamines); 333DO1RDJY (Serotonin); 37297-20-4 (phytoalexins); X8ZC7V0OX3 (Tyramine)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170703
[Lr] Data última revisão:
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170510
[St] Status:MEDLINE
[do] DOI:10.1080/09168451.2017.1290520

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