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[PMID]:28873546
[Au] Autor:Qiu S; Wang Y; Cheng Y; Liu Y; Yadav MP; Yin L
[Ad] Endereço:Beijing Key Laboratory for Food Non-thermal Processing, College of Food Science and Nutritional Engineering, China Agricultural University, P. O. Box 40, No. 17 Qinghuadonglu, Haidian, Beijing 100083, PR China; Eastern Regional Research Center, Agricultural Research Service, US Department of Agricul
[Ti] Título:Reduction of biogenic amines in sufu by ethanol addition during ripening stage.
[So] Source:Food Chem;239:1244-1252, 2018 Jan 15.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The aim of this study was to investigate the content of biogenic amines (BAs) in different types of sufu samples obtained from different producers, and the effect of ethanol in reducing BA levels during sufu ripening. The results showed that different manufacturing processes altered the distribution of BAs in commercial sufu. Putrescine, cadaverine, histamine, and tryptamine were the main and common BAs in red, white and grey sufu. The contents of putrescine, cadaverine, tryptamine, ß-phenylethylamine and tyramine in the grey sufu of all producer brands were significantly (p<0.05) higher than those in the white and red sufu. The addition of ethanol to the dressing mixture had a significant influence in reducing the total content of BAs in laboratory-made sufu. The slight increase in polypeptide and amino nitrogen contents after the addition of ethanol indicated a reduction in the degradation of water soluble protein.
[Mh] Termos MeSH primário: Aminas Biogênicas/química
[Mh] Termos MeSH secundário: Cadaverina
Cromatografia Líquida de Alta Pressão
Etanol
Fenetilaminas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biogenic Amines); 0 (Phenethylamines); 327C7L2BXQ (phenethylamine); 3K9958V90M (Ethanol); L90BEN6OLL (Cadaverine)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170907
[St] Status:MEDLINE


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[PMID]:28945762
[Au] Autor:Solomon-Zemler R; Sarfstein R; Werner H
[Ad] Endereço:Department of Human Molecular Genetics and Biochemistry, Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel.
[Ti] Título:Nuclear insulin-like growth factor-1 receptor (IGF1R) displays proliferative and regulatory activities in non-malignant cells.
[So] Source:PLoS One;12(9):e0185164, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The insulin-like growth factor-1 receptor (IGF1R) mediates the biological actions of IGF1 and IGF2. The IGF1R is involved in both physiological and pathological activities and is usually overexpressed in most types of cancer. In addition to its classical mechanism of action, recent evidence has shown a nuclear presence of IGF1R, associated with novel genomic/transcriptional types of activities. The present study was aimed at evaluating the hypothesis that nuclear IGF1R localization is not restricted to cancer cells and might constitute a novel physiologically relevant regulatory mechanism. Our data shows that nuclear translocation takes place in a wide array of cells, including normal diploid fibroblasts. In addition, we provide evidence for a synergistic effect of a nuclear translocation blocker along with selective IGF1R inhibitors in terms of decreasing cell proliferation. Given the important role of the IGF1R in mitogenesis, the present results may be of translational relevance in cancer research. In conclusion, results are consistent with the concept that nuclear IGF1R fulfills important physiological and pathological roles.
[Mh] Termos MeSH primário: Proliferação Celular/fisiologia
Receptores de Somatomedina/fisiologia
[Mh] Termos MeSH secundário: Transporte Ativo do Núcleo Celular/efeitos dos fármacos
Cadaverina/análogos & derivados
Cadaverina/farmacologia
Linhagem Celular
Linhagem Celular Tumoral
Movimento Celular/efeitos dos fármacos
Movimento Celular/fisiologia
Núcleo Celular/fisiologia
Proliferação Celular/efeitos dos fármacos
Transformação Celular Neoplásica
Células Cultivadas
Fibroblastos/metabolismo
Técnicas de Silenciamento de Genes
Seres Humanos
Células MCF-7
Microscopia Confocal
Receptores de Somatomedina/antagonistas & inibidores
Receptores de Somatomedina/genética
Transdução de Sinais
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (IGF1R protein, human); 0 (Receptors, Somatomedin); I9N81SC5HD (monodansylcadaverine); L90BEN6OLL (Cadaverine)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171023
[Lr] Data última revisão:
171023
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170926
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0185164


  3 / 1092 MEDLINE  
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[PMID]:28931053
[Au] Autor:Deng J; Gao H; Gao Z; Zhao H; Yang Y; Wu Q; Wu B; Jiang C
[Ad] Endereço:State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Life Science and Technology, Guangxi University, Nanning, Guangxi, China.
[Ti] Título:Identification and molecular characterization of a metagenome-derived L-lysine decarboxylase gene from subtropical soil microorganisms.
[So] Source:PLoS One;12(9):e0185060, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:L-lysine decarboxylase (LDC, EC 4.1.1.18) is a key enzyme in the decarboxylation of L-lysine to 1,5-pentanediamine and efficiently contributes significance to biosynthetic capability. Metagenomic technology is a shortcut approach used to obtain new genes from uncultured microorganisms. In this study, a subtropical soil metagenomic library was constructed, and a putative LDC gene named ldc1E was isolated by function-based screening strategy through the indication of pH change by L-lysine decarboxylation. Amino acid sequence comparison and homology modeling indicated the close relation between Ldc1E and other putative LDCs. Multiple sequence alignment analysis revealed that Ldc1E contained a highly conserved motif Ser-X-His-Lys (Pxl), and molecular docking results showed that this motif was located in the active site and could combine with the cofactor pyridoxal 5'-phosphate. The ldc1E gene was subcloned into the pET-30a(+) vector and highly expressed in Escherichia coli BL21 (DE3) pLysS. The recombinant protein was purified to homogeneity. The maximum activity of Ldc1E occurred at pH 6.5 and 40°C using L-lysine monohydrochloride as the substrate. Recombinant Ldc1E had apparent Km, kcat, and kcat/Km values of 1.08±0.16 mM, 5.09±0.63 s-1, and 4.73×103 s-1 M-1, respectively. The specific activity of Ldc1E was 1.53±0.06 U mg-1 protein. Identifying a metagenome-derived LDC gene provided a rational reference for further gene modifications in industrial applications.
[Mh] Termos MeSH primário: Carboxiliases/genética
Carboxiliases/metabolismo
Metagenoma
Microbiologia do Solo
[Mh] Termos MeSH secundário: Cadaverina/biossíntese
Carboxiliases/química
Domínio Catalítico
China
Clima
Clonagem Molecular
Escherichia coli/genética
Concentração de Íons de Hidrogênio
Metais/química
Conformação Proteica
Homologia Estrutural de Proteína
Especificidade por Substrato
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Metals); EC 4.1.1.- (Carboxy-Lyases); EC 4.1.1.18 (lysine decarboxylase); L90BEN6OLL (Cadaverine)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171017
[Lr] Data última revisão:
171017
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170921
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0185060


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[PMID]:28877265
[Au] Autor:Cheng B; Morales LD; Zhang Y; Mito S; Tsin A
[Ad] Endereço:Department of Biomedical Science, School of Medicine, University of Texas Rio Grande Valley, Edinburg, Texas, United States of America.
[Ti] Título:Niclosamide induces protein ubiquitination and inhibits multiple pro-survival signaling pathways in the human glioblastoma U-87 MG cell line.
[So] Source:PLoS One;12(9):e0184324, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Glioblastoma is the most common and lethal malignant primary brain tumor for which the development of efficacious chemotherapeutic agents remains an urgent need. The anti-helminthic drug niclosamide, which has long been in use to treat tapeworm infections, has recently attracted renewed interest due to its apparent anticancer effects in a variety of in vitro and in vivo cancer models. However, the mechanism(s) of action remains to be elucidated. In the present study, we found that niclosamide induced cell toxicity in human glioblastoma cells corresponding with increased protein ubiquitination, ER stress and autophagy. In addition, niclosamide treatment led to down-regulation of Wnt/ß-catenin, PI3K/AKT, MAPK/ERK, and STAT3 pro-survival signal transduction pathways to further reduce U-87 MG cell viability. Taken together, these results provide new insights into the glioblastoma suppressive capabilities of niclosamide, showing that niclosamide can target multiple major cell signaling pathways simultaneously to effectively promote cell death in U-87 MG cells. Niclosamide constitutes a new prospect for a therapeutic treatment against human glioblastoma.
[Mh] Termos MeSH primário: Antineoplásicos/farmacologia
Neoplasias Encefálicas/metabolismo
Glioblastoma/metabolismo
Niclosamida/farmacologia
Ubiquitinação
[Mh] Termos MeSH secundário: Anti-Helmínticos/química
Apoptose
Neoplasias Encefálicas/tratamento farmacológico
Cadaverina/análogos & derivados
Cadaverina/química
Linhagem Celular Tumoral
Proliferação Celular
Sobrevivência Celular
Ensaios de Seleção de Medicamentos Antitumorais
Glioblastoma/tratamento farmacológico
Seres Humanos
Fator de Transcrição STAT3/metabolismo
Transdução de Sinais
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anthelmintics); 0 (Antineoplastic Agents); 0 (STAT3 Transcription Factor); 0 (STAT3 protein, human); 8KK8CQ2K8G (Niclosamide); I9N81SC5HD (monodansylcadaverine); L90BEN6OLL (Cadaverine)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171023
[Lr] Data última revisão:
171023
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170907
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0184324


  5 / 1092 MEDLINE  
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[PMID]:28690305
[Au] Autor:Handa A; Kawanabe H; Ibe A
[Ad] Endereço:Jissen Women's University.
[Ti] Título:Determination of Nonvolatile Amines in Foods by Improved Dansyl Derivatization Reaction.
[So] Source:Shokuhin Eiseigaku Zasshi;58(3):149-154, 2017.
[Is] ISSN:1882-1006
[Cp] País de publicação:Japan
[La] Idioma:jpn
[Ab] Resumo:An analytical method for the determination of nonvolatile amines (putrescine, cadaverine, histamine, tyramine, and spermidine) in foods was developed, using an improved dansyl derivatization technique. The five amines were extracted from food with 1% trichloroacetic acid. Three milliliter of extract was applied to a polymer-based strong cation exchange resin mini-column, which was washed with 5 mL of water, and eluted with 5 mL of 1 mol/L potassium carbonate solution. The eluate was dansylated, then 5 mL of toluene was added with shaking. The toluene layer was evaporated. The residue was taken up in 1 mL of acetonitrile and shaken with 1 mL of 5% proline in 1 mol/L potassium carbonate solution. The upper acetonitrile layer was collected, filtered, and subjected to HPLC. The limits of quantitation for putrescine and cadaverine in the samples were both 0.2 µg/g; those of spermidine, tyramine, and histamine were 0.8, 2.0, and 5.0 µg/g, respectively. The average recoveries of the five amines from nine foods exceeded 80%.
[Mh] Termos MeSH primário: Cromatografia Líquida de Alta Pressão/métodos
Compostos de Dansil
Análise de Alimentos/métodos
Histamina/análise
Tiramina/análise
[Mh] Termos MeSH secundário: Acetonitrilos
Cadaverina/análise
Cadaverina/isolamento & purificação
Histamina/isolamento & purificação
Putrescina/análise
Putrescina/isolamento & purificação
Extração em Fase Sólida/métodos
Soluções
Espermidina/análise
Espermidina/isolamento & purificação
Tolueno
Ácido Tricloroacético
Tiramina/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acetonitriles); 0 (Dansyl Compounds); 0 (Solutions); 3FPU23BG52 (Toluene); 5V2JDO056X (Trichloroacetic Acid); 820484N8I3 (Histamine); L90BEN6OLL (Cadaverine); QMU9166TJ4 (dansyl chloride); U87FK77H25 (Spermidine); V10TVZ52E4 (Putrescine); X8ZC7V0OX3 (Tyramine); Z072SB282N (acetonitrile)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170711
[St] Status:MEDLINE
[do] DOI:10.3358/shokueishi.58.149


  6 / 1092 MEDLINE  
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[PMID]:28546427
[Au] Autor:Hobley L; Li B; Wood JL; Kim SH; Naidoo J; Ferreira AS; Khomutov M; Khomutov A; Stanley-Wall NR; Michael AJ
[Ad] Endereço:From the Departments of Pharmacology and.
[Ti] Título:Spermidine promotes biofilm formation by activating expression of the matrix regulator .
[So] Source:J Biol Chem;292(29):12041-12053, 2017 Jul 21.
[Is] ISSN:1083-351X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Ubiquitous polyamine spermidine is not required for normal planktonic growth of but is essential for robust biofilm formation. However, the structural features of spermidine required for biofilm formation are unknown and so are the molecular mechanisms of spermidine-stimulated biofilm development. We report here that in a spermidine-deficient mutant, the structural analogue norspermidine, but not homospermidine, restored biofilm formation. Intracellular biosynthesis of another spermidine analogue, aminopropylcadaverine, from exogenously supplied homoagmatine also restored biofilm formation. The differential ability of C-methylated spermidine analogues to functionally replace spermidine in biofilm formation indicated that the aminopropyl moiety of spermidine is more sensitive to -methylation, which it is essential for biofilm formation, but that the length and symmetry of the molecule is not critical. Transcriptomic analysis of a spermidine-depleted mutant uncovered a nitrogen-, methionine-, and -adenosylmethionine-sufficiency response, resulting in repression of gene expression related to purine catabolism, methionine and -adenosylmethionine biosynthesis and methionine salvage, and signs of altered membrane status. Consistent with the spermidine requirement in biofilm formation, single-cell analysis of this mutant indicated reduced expression of the operons for production of the exopolysaccharide and TasA protein biofilm matrix components and SinR antagonist Deletion of or ectopic expression of in the spermidine-deficient Δ background restored biofilm formation, indicating that spermidine is required for expression of the biofilm regulator Our results indicate that spermidine functions in biofilm development by activating transcription of the biofilm matrix exopolysaccharide and TasA operons through the regulator .
[Mh] Termos MeSH primário: Bacillus subtilis/fisiologia
Proteínas de Bactérias/agonistas
Biofilmes/crescimento & desenvolvimento
Regulação Bacteriana da Expressão Gênica
Polissacarídeos Bacterianos/biossíntese
Espermidina/metabolismo
Fatores de Transcrição/agonistas
[Mh] Termos MeSH secundário: Adenosilmetionina Descarboxilase/genética
Adenosilmetionina Descarboxilase/metabolismo
Bacillus subtilis/citologia
Bacillus subtilis/genética
Bacillus subtilis/crescimento & desenvolvimento
Proteínas de Bactérias/genética
Proteínas de Bactérias/metabolismo
Cadaverina/análogos & derivados
Cadaverina/metabolismo
Deleção de Genes
Perfilação da Expressão Gênica
Metionina/metabolismo
Metilação
Ciclo do Nitrogênio
Óperon
Purinas/metabolismo
S-Adenosilmetionina/metabolismo
Análise de Célula Única
Espermidina/análogos & derivados
Fatores de Transcrição/genética
Fatores de Transcrição/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Polysaccharides, Bacterial); 0 (Purines); 0 (Transcription Factors); 0 (exopolysaccharide, Bacillus); 56-18-8 (norspermidine); 56-19-9 (N-(3-aminopropyl)cadaverine); 7LP2MPO46S (S-Adenosylmethionine); AE28F7PNPL (Methionine); EC 4.1.1.50 (Adenosylmethionine Decarboxylase); L90BEN6OLL (Cadaverine); U87FK77H25 (Spermidine)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170808
[Lr] Data última revisão:
170808
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170527
[St] Status:MEDLINE
[do] DOI:10.1074/jbc.M117.789644


  7 / 1092 MEDLINE  
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[PMID]:28530604
[Au] Autor:Ngapo TM; Vachon L
[Ad] Endereço:Food Research and Development Centre, Agriculture and Agri-Food Canada, 3600 boulevard Casavant Ouest, St-Hyacinthe, Québec J2S 8E3, Canada. Electronic address: tania.ngapo@agr.gc.ca.
[Ti] Título:Biogenic amine concentrations and evolution in "chilled" Canadian pork for the Japanese market.
[So] Source:Food Chem;233:500-506, 2017 Oct 15.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The aim of this study was to evaluate concentrations and evolution of biogenic amines in Canadian pork destined for the Japanese market. At 48h post-mortem, export quality loins were aged at -1.7°C for 13, 28, 43 or 58d (chilled) or 4.0°C for 5d (fresh). Increasing concentrations of putrescine, spermine and spermidine were observed with chilled ageing period and were greater in chilled export (43d at -1.7°C) than domestic market (5d at 4.0°C) pork equivalents. Cadaverine was detected, but was not influenced by ageing conditions, and tyramine was only detected in some samples after 43days at -1.7°C. Individual biogenic amines were not correlated with their precursor amino acids. Biogenic amines in Canadian pork for the chilled export Japanese market were not in sufficiently high concentrations to pose a risk of intoxication.
[Mh] Termos MeSH primário: Aminas Biogênicas/análise
Carne Vermelha/análise
[Mh] Termos MeSH secundário: Animais
Cadaverina
Canadá
Putrescina
Espermidina
Espermina
Suínos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biogenic Amines); 2FZ7Y3VOQX (Spermine); L90BEN6OLL (Cadaverine); U87FK77H25 (Spermidine); V10TVZ52E4 (Putrescine)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170808
[Lr] Data última revisão:
170808
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170523
[St] Status:MEDLINE


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[PMID]:28271852
[Au] Autor:Zhang Y; Li D; Lv J; Li Q; Kong C; Luo Y
[Ad] Endereço:Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, PR China.
[Ti] Título:Effect of cinnamon essential oil on bacterial diversity and shelf-life in vacuum-packaged common carp (Cyprinus carpio) during refrigerated storage.
[So] Source:Int J Food Microbiol;249:1-8, 2017 May 16.
[Is] ISSN:1879-3460
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The present study investigated the effect of cinnamon essential oil on the quality of vacuum-packaged common carp (Cyprinus carpio) fillets stored at 4±1°C in terms of sensory scores, physicochemical characteristics (total volatile basic nitrogen (TVB-N), biogenic amines, and color), and presence of spoilage microbiota. A total of 290,753 bacterial sequences and 162 different genera belonging to 14 phyla were observed by a high-throughput sequencing technique targeting the V3-V4 region of 16S rDNA, which showed a more comprehensive estimate of microbial diversity in carp samples compared with microbial enumeration. Before storage, Macrococcus and Aeromonas were the prevalent populations in the control samples, but cinnamon essential oil decreased the relative abundance of Macrococcus in the treated samples. Variability in the predominant microbiota in different samples during chilled storage was observed. Aeromonas followed by Lactococcus were the major contaminants in the spoiled control samples. Microbial enumeration also observed relatively higher counts of Aeromonas than other spoilage microorganisms. Compared with the control samples, cinnamon essential oil inhibited the growth of Aeromonas and Lactococcus were the predominant components in the treated samples on day 10; plate counts also revealed a relatively high level of lactic acid bacteria during refrigerated storage. However, there were no significant differences (P>0.05) in the composition of dominant microbiota between these two treatments at the end of the shelf-life. Furthermore, cinnamon essential oil treatment was more effective in inhibiting the increase of TVB-N and the accumulation of biogenic amines (especially for putrescine and cadaverine levels). Based primarily on sensory analysis, the use of cinnamon essential oil extended the shelf-life of vacuum-packaged common carp fillets by about 2days.
[Mh] Termos MeSH primário: Cadaverina/farmacologia
Conservação de Alimentos/métodos
Armazenamento de Alimentos/métodos
Óleos Voláteis/farmacologia
Putrescina/farmacologia
Alimentos Marinhos/microbiologia
[Mh] Termos MeSH secundário: Aeromonas/efeitos dos fármacos
Aeromonas/isolamento & purificação
Animais
Carpas
Cinnamomum zeylanicum/metabolismo
Microbiologia de Alimentos
Embalagem de Alimentos/métodos
Seres Humanos
Lactococcus/efeitos dos fármacos
Lactococcus/isolamento & purificação
Microbiota/efeitos dos fármacos
Tipagem Molecular
Nitrogênio/análise
RNA Ribossômico 16S/genética
Refrigeração
Staphylococcaceae/efeitos dos fármacos
Staphylococcaceae/isolamento & purificação
Vácuo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Oils, Volatile); 0 (RNA, Ribosomal, 16S); L90BEN6OLL (Cadaverine); N762921K75 (Nitrogen); V10TVZ52E4 (Putrescine)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170915
[Lr] Data última revisão:
170915
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170309
[St] Status:MEDLINE


  9 / 1092 MEDLINE  
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[PMID]:28258224
[Au] Autor:Maki K; Shibata T; Kawabata SI
[Ad] Endereço:From the Graduate School of Systems Life Sciences.
[Ti] Título:Transglutaminase-catalyzed incorporation of polyamines masks the DNA-binding region of the transcription factor Relish.
[So] Source:J Biol Chem;292(15):6369-6380, 2017 Apr 14.
[Is] ISSN:1083-351X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In , the final immune deficiency (IMD) pathway-dependent signal is transmitted through proteolytic conversion of the nuclear factor-κB (NF-κB)-like transcription factor Relish to the active N-terminal fragment Relish-N. Relish-N is then translocated from the cytosol into the nucleus for the expression of IMD-controlled genes. We previously demonstrated that transglutaminase (TG) suppresses the IMD pathway by polymerizing Relish-N to inhibit its nuclear translocation. Conversely, we also demonstrated that orally ingested synthetic amines, such as monodansylcadaverine (DCA) and biotin-labeled pentylamine, are TG-dependently incorporated into Relish-N, causing the nuclear translocation of modified Relish-N in gut epithelial cells. It remains unclear, however, whether polyamine-containing Relish-N retains transcriptional activity. Here, we used mass spectrometry analysis of a recombinant Relish-N modified with DCA by TG activity after proteolytic digestion and show that the DCA-modified Gln residues are located in the DNA-binding region of Relish-N. TG-catalyzed DCA incorporation inhibited binding of Relish-N to the Rel-responsive element in the NF-κB-binding DNA sequence. Subcellular fractionation of TG-expressing S2 cells indicated that TG was localized in both the cytosol and nucleus. Of note, natural polyamines, including spermidine and spermine, competitively inhibited TG-dependent DCA incorporation into Relish-N. Moreover, experiments demonstrated that Relish-N was modified by spermine and that this modification reduced transcription of IMD pathway-controlled and genes. These findings suggest that intracellular TG regulates Relish-N-mediated transcriptional activity by incorporating polyamines into Relish-N and via protein-protein cross-linking.
[Mh] Termos MeSH primário: Cadaverina/análogos & derivados
Núcleo Celular/metabolismo
Proteínas de Drosophila/metabolismo
Células Epiteliais/metabolismo
Intestinos/metabolismo
Fatores de Transcrição/metabolismo
Transglutaminases/metabolismo
[Mh] Termos MeSH secundário: Transporte Ativo do Núcleo Celular/fisiologia
Animais
Peptídeos Catiônicos Antimicrobianos/biossíntese
Peptídeos Catiônicos Antimicrobianos/genética
Cadaverina/metabolismo
Linhagem Celular
Núcleo Celular/genética
Citosol/metabolismo
Proteínas de Drosophila/biossíntese
Proteínas de Drosophila/genética
Drosophila melanogaster
Células Epiteliais/citologia
Intestinos/citologia
Domínios Proteicos
Fatores de Transcrição/genética
Transglutaminases/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antimicrobial Cationic Peptides); 0 (Drosophila Proteins); 0 (Relish protein, Drosophila); 0 (Transcription Factors); 0 (diptericin B protein, Drosophila); 80451-04-3 (cecropin A); EC 2.3.2.13 (Transglutaminases); I9N81SC5HD (monodansylcadaverine); L90BEN6OLL (Cadaverine)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170428
[Lr] Data última revisão:
170428
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170305
[St] Status:MEDLINE
[do] DOI:10.1074/jbc.M117.779579


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[PMID]:28221983
[Au] Autor:Li Q; Lv J; Zhang L; Dong Z; Feng L; Luo Y
[Ad] Endereço:Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, People's Republic of China.
[Ti] Título:Biogenic Amines and Predictive Models of Quality of Rainbow Trout ( Oncorhynchus mykiss ) Fillets during Storage.
[So] Source:J Food Prot;80(2):279-287, 2017 Feb.
[Is] ISSN:1944-9097
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:To estimate biogenic amines and changes in quality of rainbow trout ( Oncorhynchus mykiss ) fillets at different temperatures, we determined the sensory attributes, total volatile basic nitrogen (TVB-N), total viable counts (TVC), and biogenic amines (BAs) of samples that were untreated (CK) or dry cured with 1.8% salt (T). There was no significant difference between CK and T samples in terms of TVB-N, TVC, and BAs. TVB-N and TVC increased significantly (P < 0.05) with storage time at 3, 9, and 15°C. Putrescine (PUT) and cadaverine (CAD) increased significantly (P < 0.05) at -3, 3, 9, and 15°C during storage. Histamine formed more easily when storage temperatures were higher. The kinetic models of sensory scores for TVB-N, TVC, PUT, CAD, and the sum of PUT and CAD (PUT+CAD) in T samples versus storage time and temperature were developed based on the Arrhenius equation. High regression coefficients (R > 0.9) indicated the acceptability of the kinetic model for predicting changes in the quality of the rainbow trout fillets. Relative errors between predicted and experimental values of TVB-N, TVC, and PUT+CAD were all within 10% except for TVB-N on day 6. The prediction model based on TVB-N, TVC, and PUT+CAD can be applied to evaluate changes in quality of rainbow trout fillets from -3 to 15°C (270 to 288 K).
[Mh] Termos MeSH primário: Aminas Biogênicas
Oncorhynchus mykiss
[Mh] Termos MeSH secundário: Animais
Cadaverina
Conservação de Alimentos
Putrescina
Cloreto de Sódio
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biogenic Amines); 451W47IQ8X (Sodium Chloride); L90BEN6OLL (Cadaverine); V10TVZ52E4 (Putrescine)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170605
[Lr] Data última revisão:
170605
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170222
[St] Status:MEDLINE
[do] DOI:10.4315/0362-028X.JFP-16-136



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