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[PMID]:29293658
[Au] Autor:Slachmuylders L; Van Acker H; Brackman G; Sass A; Van Nieuwerburgh F; Coenye T
[Ad] Endereço:Laboratory of Pharmaceutical Microbiology, Ghent University, Ghent, Belgium.
[Ti] Título:Elucidation of the mechanism behind the potentiating activity of baicalin against Burkholderia cenocepacia biofilms.
[So] Source:PLoS One;13(1):e0190533, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Reduced antimicrobial susceptibility due to resistance and tolerance has become a serious threat to human health. An approach to overcome this reduced susceptibility is the use of antibiotic adjuvants, also known as potentiators. These are compounds that have little or no antibacterial effect on their own but increase the susceptibility of bacterial cells towards antimicrobial agents. Baicalin hydrate, previously described as a quorum sensing inhibitor, is such a potentiator that increases the susceptibility of Burkholderia cenocepacia J2315 biofilms towards tobramycin. The goal of the present study is to elucidate the molecular mechanisms behind the potentiating activity of baicalin hydrate and related flavonoids. We first determined the effect of multiple flavonoids on susceptibility of B. cenocepacia J2315 towards tobramycin. Increased antibiotic susceptibility was most pronounced in combination with apigenin 7-O-glucoside and baicalin hydrate. For baicalin hydrate, also other B. cepacia complex strains and other antibiotics were tested. The potentiating effect was only observed for aminoglycosides and was both strain- and aminoglycoside-dependent. Subsequently, gene expression was compared between baicalin hydrate treated and untreated cells, in the presence and absence of tobramycin. This revealed that baicalin hydrate affected cellular respiration, resulting in increased reactive oxygen species production in the presence of tobramycin. We subsequently showed that baicalin hydrate has an impact on oxidative stress via several pathways including oxidative phosphorylation, glucarate metabolism and by modulating biosynthesis of putrescine. Furthermore, our data strongly suggest that the influence of baicalin hydrate on oxidative stress is unrelated to quorum sensing. Our data indicate that the potentiating effect of baicalin hydrate is due to modulating the oxidative stress response, which in turn leads to increased tobramycin-mediated killing.
[Mh] Termos MeSH primário: Biofilmes/efeitos dos fármacos
Burkholderia cenocepacia/efeitos dos fármacos
Flavonoides/farmacologia
[Mh] Termos MeSH secundário: Burkholderia cenocepacia/genética
Burkholderia cenocepacia/crescimento & desenvolvimento
Contagem de Colônia Microbiana
Regulação Bacteriana da Expressão Gênica/efeitos dos fármacos
Genes Bacterianos
Testes de Sensibilidade Microbiana
Estresse Oxidativo/efeitos dos fármacos
Putrescina/metabolismo
Percepção de Quorum/efeitos dos fármacos
Transcriptoma
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Flavonoids); 347Q89U4M5 (baicalin); V10TVZ52E4 (Putrescine)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180221
[Lr] Data última revisão:
180221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180103
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190533


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[PMID]:28927792
[Au] Autor:Cai YS; Sarotti AM; Gündisch D; Kondratyuk TP; Pezzuto JM; Turkson J; Cao S
[Ad] Endereço:Department of Pharmaceutical Sciences, Daniel K. Inouye College of Pharmacy, University of Hawaii at Hilo, 200 West Kawili Street, Hilo, HI 96720, United States; Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, School of Pharmaceutical Sciences, Wuhan Universit
[Ti] Título:Heliotropiumides A and B, new phenolamides with N-carbamoyl putrescine moiety from Heliotropium foertherianum collected in Hawaii and their biological activities.
[So] Source:Bioorg Med Chem Lett;27(20):4630-4634, 2017 10 15.
[Is] ISSN:1464-3405
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Two new compounds heliotropiumides A (1) and B (2), phenolamides each with an uncommon carbamoyl putrescine moiety, were isolated from the seeds of a naturalized Hawaiian higher plant, Heliotropium foertherianum Diane & Hilger in the borage family, which is widely used for the treatment of ciguatera fish poisoning. The structures of compounds 1 and 2 were characterized based on MS spectroscopic and NMR analysis, and DP4+ calculations. The absolute configuration (AC) of compound 1 was determined by comparison of its optical rotation with those reported in literature. Compound 2 showed inhibition against NF-κB with an IC value of 36µM.
[Mh] Termos MeSH primário: Amidas/farmacologia
Benzofuranos/química
Heliotropium/química
Fenóis/química
Extratos Vegetais/química
Extratos Vegetais/farmacologia
[Mh] Termos MeSH secundário: Amidas/química
Amidas/toxicidade
Anti-Infecciosos/química
Anti-Infecciosos/isolamento & purificação
Anti-Infecciosos/farmacologia
Antineoplásicos Alquilantes/química
Antineoplásicos Alquilantes/isolamento & purificação
Antineoplásicos Alquilantes/farmacologia
Benzofuranos/farmacologia
Benzofuranos/toxicidade
Linhagem Celular Tumoral
Sobrevivência Celular/efeitos dos fármacos
Fungos/efeitos dos fármacos
Bactérias Gram-Negativas/efeitos dos fármacos
Bactérias Gram-Positivas/efeitos dos fármacos
Heliotropium/metabolismo
Seres Humanos
Espectroscopia de Ressonância Magnética
Conformação Molecular
NF-kappa B/antagonistas & inibidores
NF-kappa B/metabolismo
Fenóis/farmacologia
Fenóis/toxicidade
Extratos Vegetais/uso terapêutico
Putrescina/química
Intoxicação por Frutos do Mar/prevenção & controle
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Amides); 0 (Anti-Infective Agents); 0 (Antineoplastic Agents, Alkylating); 0 (Benzofurans); 0 (NF-kappa B); 0 (Phenols); 0 (Plant Extracts); 0 (heliotropiumide A); 0 (heliotropiumide B); V10TVZ52E4 (Putrescine)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171125
[Lr] Data última revisão:
171125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170921
[St] Status:MEDLINE


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[PMID]:28901905
[Au] Autor:Lin SY; Hameed A; Wen CZ; Hsu YH; Liu YC; Lai WA; Young CC
[Ad] Endereço:1​Department of Soil and Environmental Sciences, College of Agriculture and Natural Resources, National Chung Hsing University, Taichung, Taiwan, ROC.
[Ti] Título:Hydrogenophaga aquatica sp. nov., isolated from a hot spring.
[So] Source:Int J Syst Evol Microbiol;67(10):3716-3721, 2017 Oct.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A polyphasic approach was used to characterize an aerobic, Gram-negative, rod-shaped bacterium (designated strain CC-KL-3 ) isolated from a hot spring. Phylogenetic analyses based on 16S rRNA genes indicated that strain CC-KL-3 showed highest sequence similarity to Hydrogenophaga bisanensis (97.7 %) and Hydrogenophaga atypica (97.6 %) and lower sequence similarity to other species (less than 97.6 %). The levels of DNA-DNA relatedness between strain CC-KL-3 , H. bisanensis and H. atypica were estimated to be 13.0 and 8.7 % (the reciprocal value was 14.7 and 6.3 %). Strain CC-KL-3 was non-motile, without apparent flagella and able to grow between 15-42 °C (optimal 30 °Ð¡), pH 6.0-8.0 (optimal 7.0) and 0-2 % (w/v) NaCl (optimal 0 %). The DNA G+C content was 61.4 mol% and the major quinone system was ubiquinone (Q-8). The polyamine profile revealed the predominance of 2-hydroxyputrescine and putrescine and the dominant cellular fatty acids were C16 : 0 (28.9 %), C16 : 1ω7c/C16 : 1ω6c (41.4 %) and C18 : 1ω7c/C18 : 1ω6c (11.9 %). These data corroborated the affiliation of strain CC-KL-3 to the genus Hydrogenophaga. Based on the distinct phylogenetic, phenotypic and chemotaxonomic traits, and the results of comparative 16S rRNA gene sequence analysis, strain CC-KL-3 is considered to represent a novel species of the genus Hydrogenophaga, affiliated to the family Comamonadaceae, for which the name Hydrogenophaga aquatica sp. nov. is proposed. The type strain is CC-KL-3 (=BCRC 80937 =JCM 31216 ).
[Mh] Termos MeSH primário: Comamonadaceae/classificação
Fontes Termais/microbiologia
Filogenia
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
Comamonadaceae/genética
Comamonadaceae/isolamento & purificação
DNA Bacteriano/genética
Ácidos Graxos/química
Hibridização de Ácido Nucleico
Putrescina/análogos & derivados
Putrescina/química
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Taiwan
Ubiquinona/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (RNA, Ribosomal, 16S); 1339-63-5 (Ubiquinone); 539-59-3 (2-hydroxyputrescine); CQA993F7P8 (ubiquinone 8); V10TVZ52E4 (Putrescine)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171027
[Lr] Data última revisão:
171027
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170914
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002146


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[PMID]:28820094
[Au] Autor:Altankhuu K; Kim J
[Ad] Endereço:Department of Life Science, College of Natural Sciences, Kyonggi University, Suwon, Gyeonggi-Do 16227, Republic of Korea.
[Ti] Título:Massilia solisilvae sp. nov., Massilia terrae sp. nov. and Massilia agilis sp. nov., isolated from forest soil in South Korea by using a newly developed culture method.
[So] Source:Int J Syst Evol Microbiol;67(8):3026-3032, 2017 Aug.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Using a newly developed culture method for not yet cultured soil bacteria, three Gram-stain-negative, aerobic, non-spore-forming, motile, and rod-shaped bacteria (strain designated J18T, J11T and J9T) were isolated from forest soil at Kyonggi University, South Korea. Isolates were subjected to a taxonomic study by using a polyphasic approach. According to a phylogenetic tree based on 16S rRNA gene sequences, strains J18T, J11T and J9T belonged to the genus Massilia and clustered with Massilia haematophila CCUG 38318T (similarity range: 97.6~98.0 %). The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol, and the genomic DNA G+C contents of strains J18T, J11T and J9T were 63.4, 68.7 and 64.5 mol%, respectively. The major polyamines were putrescine and 2-hydroxyputescine, which were detected in all three strains. DNA-DNA between the three tested strains and the reference strains much lower than 70 %, the recommended threshold value for the delineation of genomic species. The predominant respiratory quinine was ubiquinone-8 (Q-8) and the major cellular fatty acids were Summed feature 3 (C16 : 1ω6c/C16 : 1ω7c) and C16 : 0. On the basis of phenotypic and genotypic data and DNA-DNA hybridization results, the three isolates are considered to represent three novel species of the genus Massilia, for which the names Massilia solisilvae sp. nov. for type strain J18T (=KEMB 9005-366T=JCM 31607T), Massilia terrae sp. nov. for type strain J11T (=KEMB 9005-360T=JCM 31606T) and Massilia agilis sp. nov. for type strain J9T (=KEMB 9005-359T=JCM 31605T) are proposed.
[Mh] Termos MeSH primário: Florestas
Oxalobacteraceae/classificação
Filogenia
Microbiologia do Solo
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
DNA Bacteriano/genética
Ácidos Graxos/química
Hibridização de Ácido Nucleico
Oxalobacteraceae/genética
Oxalobacteraceae/isolamento & purificação
Fosfolipídeos/química
Putrescina/química
RNA Ribossômico 16S/genética
República da Coreia
Análise de Sequência de DNA
Ubiquinona/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S); 1339-63-5 (Ubiquinone); CQA993F7P8 (ubiquinone 8); V10TVZ52E4 (Putrescine)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170919
[Lr] Data última revisão:
170919
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170819
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002076


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[PMID]:28707668
[Au] Autor:Masalova OV; Lesnova EI; Samokhvalov EI; Permyakova KY; Ivanov AV; Kochetkov SN; Kushch AA
[Ad] Endereço:Ivanovsky Institute of Virology, Gamaleya Federal Research Center of Epidemiology and Microbiology, Ministry of Health of the Russian Federation, Moscow, 123098 Russia.
[Ti] Título:[Low-molecular-weight regulators of biogenic polyamine metabolism affect cytokine production and expression of hepatitis С virus proteins in Huh7.5 human hepatocarcinoma cells].
[So] Source:Mol Biol (Mosk);51(3):512-523, 2017 May-Jun.
[Is] ISSN:0026-8984
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:Hepatitis C virus (HCV) induces the expression of the genes of proinflammatory cytokines, the excessive production of which may cause cell death, and contribute to development of liver fibrosis and hepatocarcinoma. The relationship between cytokine production and metabolic disorders in HCV-infected cells remains obscure. The levels of biogenic polyamines, spermine, spermidine, and their precursor putrescine, may be a potential regulator of these processes. The purpose of the present work was to study the effects of the compounds which modulate biogenic polyamines metabolism on cytokine production and HCV proteins expression. Human hepatocarcinoma Huh7.5 cells have been transfected with the plasmids that encode HCV proteins and further incubated with the following low-molecular compounds that affect different stages of polyamine metabolism: (1) difluoromethylornithine (DFMO), the inhibitor of ornithine decarboxylase, the enzyme that catalyzes the biosynthesis of polyamines; (2) N,N'-bis(2,3-butane dienyl)-1,4-diaminobutane (MDL72.527), the inhibitor of proteins involved in polyamine degradation; and (3) synthetic polyamine analog N^(I),N^(II)-diethylnorspermine (DENSpm), an inducer of polyamine degradation enzyme. The intracellular accumulation and secretion of cytokines (IL-6, IL-1ß, TNF-α, and TGF-ß) was assessed by immunocytochemistry and in the immunoenzyme assay, while the cytokine gene expression was studied using reverse transcription and PCR. The effects of the compounds under analysis on the expression of HCV proteins were analyzed using the indirect immunofluorescence with anti-HCV monoclonal antibodies. It has been demonstrated that, in cells transfected with HCV genes, DFMO reduces the production of three out of four tested cytokines, namely, TNF-α and TGF-ß in cells that express HCV core, Е1Е2, NS3, NS5A, and NS5B proteins, and IL-1ß in the cells that express HCV core, Е1Е2, and NS3 proteins. MDL72527 and DENSpm decreased cytokine production to a lesser extent. Incubation with DFMO led to a 28-32% decrease in the number of cells expressing NS5B or NS5A, both of which are key components of the HCV replication complex. The results obtained in the work indicate that a further detailed study of the antiviral activity of DFMO is required in order to assess its potential as an anti-hepatitis C therapeutic agent.
[Mh] Termos MeSH primário: Citocinas/biossíntese
Eflornitina/farmacologia
Hepacivirus/genética
Hepatite/tratamento farmacológico
[Mh] Termos MeSH secundário: Poliaminas Biogênicas/metabolismo
Linhagem Celular Tumoral
Regulação Viral da Expressão Gênica/efeitos dos fármacos
Hepacivirus/efeitos dos fármacos
Hepatite/genética
Hepatite/virologia
Seres Humanos
Inibidores da Ornitina Descarboxilase/farmacologia
Putrescina/biossíntese
Espermidina/biossíntese
Espermina/biossíntese
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biogenic Polyamines); 0 (Cytokines); 0 (Ornithine Decarboxylase Inhibitors); 2FZ7Y3VOQX (Spermine); U87FK77H25 (Spermidine); V10TVZ52E4 (Putrescine); ZQN1G5V6SR (Eflornithine)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171101
[Lr] Data última revisão:
171101
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170715
[St] Status:MEDLINE
[do] DOI:10.7868/S0026898417030120


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[PMID]:28690305
[Au] Autor:Handa A; Kawanabe H; Ibe A
[Ad] Endereço:Jissen Women's University.
[Ti] Título:Determination of Nonvolatile Amines in Foods by Improved Dansyl Derivatization Reaction.
[So] Source:Shokuhin Eiseigaku Zasshi;58(3):149-154, 2017.
[Is] ISSN:1882-1006
[Cp] País de publicação:Japan
[La] Idioma:jpn
[Ab] Resumo:An analytical method for the determination of nonvolatile amines (putrescine, cadaverine, histamine, tyramine, and spermidine) in foods was developed, using an improved dansyl derivatization technique. The five amines were extracted from food with 1% trichloroacetic acid. Three milliliter of extract was applied to a polymer-based strong cation exchange resin mini-column, which was washed with 5 mL of water, and eluted with 5 mL of 1 mol/L potassium carbonate solution. The eluate was dansylated, then 5 mL of toluene was added with shaking. The toluene layer was evaporated. The residue was taken up in 1 mL of acetonitrile and shaken with 1 mL of 5% proline in 1 mol/L potassium carbonate solution. The upper acetonitrile layer was collected, filtered, and subjected to HPLC. The limits of quantitation for putrescine and cadaverine in the samples were both 0.2 µg/g; those of spermidine, tyramine, and histamine were 0.8, 2.0, and 5.0 µg/g, respectively. The average recoveries of the five amines from nine foods exceeded 80%.
[Mh] Termos MeSH primário: Cromatografia Líquida de Alta Pressão/métodos
Compostos de Dansil
Análise de Alimentos/métodos
Histamina/análise
Tiramina/análise
[Mh] Termos MeSH secundário: Acetonitrilos
Cadaverina/análise
Cadaverina/isolamento & purificação
Histamina/isolamento & purificação
Putrescina/análise
Putrescina/isolamento & purificação
Extração em Fase Sólida/métodos
Soluções
Espermidina/análise
Espermidina/isolamento & purificação
Tolueno
Ácido Tricloroacético
Tiramina/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acetonitriles); 0 (Dansyl Compounds); 0 (Solutions); 3FPU23BG52 (Toluene); 5V2JDO056X (Trichloroacetic Acid); 820484N8I3 (Histamine); L90BEN6OLL (Cadaverine); QMU9166TJ4 (dansyl chloride); U87FK77H25 (Spermidine); V10TVZ52E4 (Putrescine); X8ZC7V0OX3 (Tyramine); Z072SB282N (acetonitrile)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170711
[St] Status:MEDLINE
[do] DOI:10.3358/shokueishi.58.149


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[PMID]:28648602
[Au] Autor:Zhang H; Au SWN
[Ad] Endereço:Centre for Protein Science and Crystallography, School of Life Sciences, The Chinese University of Hong Kong, Hong Kong.
[Ti] Título:Helicobacter pylori does not use spermidine synthase to produce spermidine.
[So] Source:Biochem Biophys Res Commun;490(3):861-867, 2017 Aug 26.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Helicobacter pylori is the primary pathogen associated to gastritis and gastric cancer. Growth of H. pylori depends on the availability of spermidine in vivo. Interestingly, the genome of H. pylori contains an incomplete set of genes for the classical pathway of spermidine biosynthesis. It is thus not clear whether some other genes remained in the pathway would have any functions in spermidine biosynthesis. Here, we study spermidine synthase, which is responsible for the final catalytic process in the classical route. Protein sequence alignment reveals that H. pylori SpeE (HpSpeE) lacks key residues for substrate binding. By using isothermal titration calorimetry, we show that purified recombinant HpSpeE does not interact with the putative substrates putrescine and decarboxylated S-adenosylmethionine, and the product spermidine. High performance liquid chromatography analysis further demonstrates that HpSpeE has no detectable in vitro enzymatic activity. Additionally, intracellular spermidine level in speE-null mutant strain is comparable to that in the wild type strain. Collectively, our results suggest that HpSpeE is functionally distinct from spermidine production. H. pylori may instead employ the alternative pathway for spermidine synthesis which is dominantly exploited by other human gut microbes.
[Mh] Termos MeSH primário: Helicobacter pylori/enzimologia
Helicobacter pylori/metabolismo
Espermidina Sintase/metabolismo
Espermidina/metabolismo
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Infecções por Helicobacter/microbiologia
Helicobacter pylori/química
Seres Humanos
Putrescina/metabolismo
S-Adenosilmetionina/análogos & derivados
S-Adenosilmetionina/metabolismo
Alinhamento de Sequência
Espermidina Sintase/química
Especificidade por Substrato
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
22365-13-5 (S-adenosyl-3-methylthiopropylamine); 7LP2MPO46S (S-Adenosylmethionine); EC 2.5.1.16 (Spermidine Synthase); U87FK77H25 (Spermidine); V10TVZ52E4 (Putrescine)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170818
[Lr] Data última revisão:
170818
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170627
[St] Status:MEDLINE


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[PMID]:28632113
[Au] Autor:Kämpfer P; Busse HJ; McInroy JA; Glaeser SP
[Ad] Endereço:1​Institut für Angewandte Mikrobiologie, Universität Giessen, Germany.
[Ti] Título:Paracandidimonas soli gen. nov., sp. nov., isolated from soil.
[So] Source:Int J Syst Evol Microbiol;67(6):1740-1745, 2017 Jun.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A slightly yellow-pigmented, Gram-stain-negative, rod-shaped bacterium, strain IMT-305T, was isolated from soil in Alabama, USA. Phylogenetic analysis based on the nearly full-length 16S rRNA gene sequence placed the strain in between the genera Pusillimonas, Parapusillimonas and Candidimonas with highest 16S rRNA gene sequence similarity to the type strain of Parapusillimonas granuli (97.5 %) and Candidimonas nitroreducens (97.4 %). The genomic G+C content of strain IMT-305T was 63.9 mol%. The main cellular fatty acids were C18:1ω7c, C17:0 cyclo, C16:0 and C16:1ω7c/iso-C15:0 2-OH (detected as summed feature 3). The polyamine pattern of strain IMT-305T contained the major compound putrescine and the betaproteobacterial diagnostic 2-hydroxyputrescine and the major respiratory quinone was ubiquinone Q-8. Predominant polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylserine, an unidentified aminolipid, an unidentified aminophospholipid and an unidentified lipid lacking any functional group. Based on phylogenetic, chemotaxonomic and phenotypic analyses a novel species within a new genus, Paracandidimonas soli gen. nov., sp. nov., is proposed. The type strain of Paracandidimonas soli is IMT-305T (=DSM 100048T=CIP 110902T=LMG 28740T=CCM 8599T).
[Mh] Termos MeSH primário: Alcaligenaceae/classificação
Filogenia
Microbiologia do Solo
[Mh] Termos MeSH secundário: Alabama
Alcaligenaceae/genética
Alcaligenaceae/isolamento & purificação
Técnicas de Tipagem Bacteriana
Composição de Bases
DNA Bacteriano/genética
Ácidos Graxos/química
Fosfolipídeos/química
Putrescina/análogos & derivados
Putrescina/química
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Ubiquinona/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S); 1339-63-5 (Ubiquinone); 539-59-3 (2-hydroxyputrescine); CQA993F7P8 (ubiquinone 8); V10TVZ52E4 (Putrescine)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170621
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.001852


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[PMID]:28530604
[Au] Autor:Ngapo TM; Vachon L
[Ad] Endereço:Food Research and Development Centre, Agriculture and Agri-Food Canada, 3600 boulevard Casavant Ouest, St-Hyacinthe, Québec J2S 8E3, Canada. Electronic address: tania.ngapo@agr.gc.ca.
[Ti] Título:Biogenic amine concentrations and evolution in "chilled" Canadian pork for the Japanese market.
[So] Source:Food Chem;233:500-506, 2017 Oct 15.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The aim of this study was to evaluate concentrations and evolution of biogenic amines in Canadian pork destined for the Japanese market. At 48h post-mortem, export quality loins were aged at -1.7°C for 13, 28, 43 or 58d (chilled) or 4.0°C for 5d (fresh). Increasing concentrations of putrescine, spermine and spermidine were observed with chilled ageing period and were greater in chilled export (43d at -1.7°C) than domestic market (5d at 4.0°C) pork equivalents. Cadaverine was detected, but was not influenced by ageing conditions, and tyramine was only detected in some samples after 43days at -1.7°C. Individual biogenic amines were not correlated with their precursor amino acids. Biogenic amines in Canadian pork for the chilled export Japanese market were not in sufficiently high concentrations to pose a risk of intoxication.
[Mh] Termos MeSH primário: Aminas Biogênicas/análise
Carne Vermelha/análise
[Mh] Termos MeSH secundário: Animais
Cadaverina
Canadá
Putrescina
Espermidina
Espermina
Suínos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biogenic Amines); 2FZ7Y3VOQX (Spermine); L90BEN6OLL (Cadaverine); U87FK77H25 (Spermidine); V10TVZ52E4 (Putrescine)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170808
[Lr] Data última revisão:
170808
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170523
[St] Status:MEDLINE


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[PMID]:28400003
[Au] Autor:Estiarte N; Crespo-Sempere A; Marín S; Sanchis V; Ramos AJ
[Ad] Endereço:Applied Mycology Unit, Food Technology Department, University of Lleida, UTPV-XaRTA, Agrotecnio Center, Lleida, Spain. Electronic address: nestiarte@tecal.udl.cat.
[Ti] Título:Exploring polyamine metabolism of Alternaria alternata to target new substances to control the fungal infection.
[So] Source:Food Microbiol;65:193-204, 2017 Aug.
[Is] ISSN:1095-9998
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Polyamines are essential for all living organisms as they are involved in several vital cell functions. The biosynthetic pathway of polyamines and its regulation is well established and, in this sense, the ornithine descarboxylase (ODC) enzyme acts as one of the controlling factors of the entire pathway. In this work we assessed the inhibition of the ODC with D, l-α-difluoromethylornithine (DFMO) on Alternaria alternata and we observed that fungal growth and mycotoxin production were reduced. This inhibition was not completely restored by the addition of exogenous putrescine. Actually, increasing concentrations of putrescine on the growth media negatively affected mycotoxin production, which was corroborated by the downregulation of pksJ and altR, both genes involved in mycotoxin biosynthesis. We also studied the polyamine metabolism of A. alternata with the goal of finding new targets that compromise its growth and its mycotoxin production capacity. In this sense, we tested two different polyamine analogs, AMXT-2455 and AMXT-3016, and we observed that they partially controlled A. alternata's viability in vitro and in vivo using tomato plants. Finding strategies to design new fungicide substances is becoming a matter of interest as resistance problems are emerging.
[Mh] Termos MeSH primário: Alternaria/efeitos dos fármacos
Alternaria/metabolismo
Antifúngicos/farmacologia
Lycopersicon esculentum/microbiologia
Doenças das Plantas/microbiologia
Poliaminas/metabolismo
[Mh] Termos MeSH secundário: Alternaria/genética
Alternaria/crescimento & desenvolvimento
Vias Biossintéticas/efeitos dos fármacos
Vias Biossintéticas/genética
Meios de Cultura/química
Eflornitina/farmacologia
Lycopersicon esculentum/efeitos dos fármacos
Micélio/efeitos dos fármacos
Micélio/genética
Micotoxinas/biossíntese
Ornitina Descarboxilase/metabolismo
Inibidores da Ornitina Descarboxilase/farmacologia
Poliaminas/química
Poliaminas/farmacologia
Putrescina/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antifungal Agents); 0 (Culture Media); 0 (Mycotoxins); 0 (Ornithine Decarboxylase Inhibitors); 0 (Polyamines); EC 4.1.1.17 (Ornithine Decarboxylase); V10TVZ52E4 (Putrescine); ZQN1G5V6SR (Eflornithine)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170427
[Lr] Data última revisão:
170427
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170413
[St] Status:MEDLINE



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