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[PMID]:28448961
[Au] Autor:Divya KP; Dharuman V
[Ad] Endereço:Molecular Electronics Laboratory, Department of Bioelectronics and Biosensors, Science Campus, Alagappa University, Karaikudi 630003, India.
[Ti] Título:Supported binary liposome vesicle-gold nanoparticle for enhanced label free DNA and protein sensing.
[So] Source:Biosens Bioelectron;95:168-173, 2017 Sep 15.
[Is] ISSN:1873-4235
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Supported binary liposome mixture of cationic liposome N-[1-(2,3-Dioleoyloxy)propyl]-N,N,N-trimethylammonium propane (DOTAP) and the zwitterionic liposome 1,2-Dioleoyl-sn-Glycero-3-Phosphoethanolamine (DOPE) were tethered on thiol monolayers in the absence and presence of gold nanoparticle to enhance sensor stability and sensitivity for label free DNA and protein sensing for the first time. Cysteamine hydrochloride (Cyst), 3-Mercaptopropionic acid (MPA), 11-Mercaptoundecanoic acid (MUDA) and 11-amino-1-undecane thiol (AUT) monolayers were used as tethers on gold surfaces. Electrochemical studies in the presence of [Fe(CN) ] indicate that the presence of both DOPE and AuNP decreases the electrostatic interaction between DOTAP and MPA layer during the formation of DOPE-DOTAP-AuNP (DDA) whereas they enhance the repulsive force on the Cyst and AUT monolayers. In the thiol monolayer supported DDA, the gelation of neutral lipid DOPE by the AuNP is disfavored which inturn promotes stability of vesicle structure. The membrane protein melittin's interaction with the DDA indicates the presence of intact vesicle by showing decreased charge transfer for the MUDA and AUT in the presence of [Fe(CN) ] . On the contrary, the presence of the bilayer and semi circled DDA on the MPA and cysteamine layers were confirmed by the increased redox reaction. Atomic Force Microscopic (AFM) and Transmission Electron Microscopic (TEM) images support the presence of an array like semi circled DDA on the MPA and well separated DDA vesicles on the MUDA with variable sizes. Dynamic Light Scattering (DLS) and Fourier Transform Infrared spectroscopy (FTIR) suggest effective coordination between DOPE, DOTAP and AuNP. Label free DNA hybridization sensing in presence of the negatively charged [Fe(CN) ] indicates the lowest DNA detection limit of 1×10 M with linearity range 1×10 to 1×10 M. Similarly, streptavidin sensing shows the lowest detection of 1ngml with a linear range 100ng to 1µg due to the increased reactive sites and distance.
[Mh] Termos MeSH primário: Técnicas Biossensoriais
Ácidos Nucleicos Livres/isolamento & purificação
Lipossomos/química
Proteínas/isolamento & purificação
[Mh] Termos MeSH secundário: Ácido 3-Mercaptopropiônico
Ácidos Nucleicos Livres/química
Cisteamina/química
Ácidos Graxos Monoinsaturados/química
Ouro/química
Limite de Detecção
Lipídeos/química
Nanopartículas Metálicas/química
Microscopia de Força Atômica
Microscopia Eletrônica de Transmissão
Hibridização de Ácido Nucleico
Fosfatidiletanolaminas/química
Compostos de Amônio Quaternário/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cell-Free Nucleic Acids); 0 (Fatty Acids, Monounsaturated); 0 (Lipids); 0 (Liposomes); 0 (Phosphatidylethanolamines); 0 (Proteins); 0 (Quaternary Ammonium Compounds); 2462-63-7 (dioleoyl phosphatidylethanolamine); 5UX2SD1KE2 (Cysteamine); 7440-57-5 (Gold); B03TJ3QU9M (3-Mercaptopropionic Acid); MR86K0XRQP (1,2-dioleoyloxy-3-(trimethylammonium)propane)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180214
[Lr] Data última revisão:
180214
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170428
[St] Status:MEDLINE


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[PMID]:28448444
[Au] Autor:Mariani F; Roncucci L
[Ad] Endereço:Department of Diagnostic and Clinical Medicine, and Public Health, University of Modena and Reggio Emilia, Via Del Pozzo 71, I-41125 Modena, Italy. francesco.mariani@unimore.it.
[Ti] Título:Role of the Vanins-Myeloperoxidase Axis in Colorectal Carcinogenesis.
[So] Source:Int J Mol Sci;18(5), 2017 Apr 27.
[Is] ISSN:1422-0067
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:The presence of chronic inflammation in the colonic mucosa leads to an increased risk of cancer. Among proteins involved in the regulation of mucosal inflammation and that may contribute both to structural damage of the intestinal mucosa and to intestinal carcinogenesis, there are myeloperoxidase (MPO) and vanins. The infiltration of colonic mucosa by neutrophils may promote carcinogenesis through MPO, a key enzyme contained in the lysosomes of neutrophils that regulates local inflammation and the generation of reactive oxygen species (ROS) and mutagenic species. The human vanin gene family consists of three genes: , and . All vanin molecules are pantetheinases, that hydrolyze pantetheine into pantothenic acid (vitamin B5), and cysteamine, a sulfhydryl compound. Vanin-1 loss confers an increased resistance to stress and acute intestinal inflammation, while vanin-2 regulates adhesion and transmigration of activated neutrophils. The metabolic product of these enzymes has a prominent role in the inflammation processes by affecting glutathione levels, inducing ulcers through a reduction in mucosal blood flow and oxygenation, decreasing local defense mechanisms, and in carcinogenesis by damaging DNA and regulating pathways involved in cell apoptosis, metabolism and growth, as Nrf2 and HIF-1α.
[Mh] Termos MeSH primário: Amidoidrolases/metabolismo
Neoplasias Colorretais/patologia
Peroxidase/metabolismo
[Mh] Termos MeSH secundário: Amidoidrolases/antagonistas & inibidores
Amidoidrolases/genética
Carcinogênese
Neoplasias Colorretais/tratamento farmacológico
Neoplasias Colorretais/metabolismo
Cisteamina/metabolismo
Inibidores Enzimáticos/uso terapêutico
Seres Humanos
Inflamação
Peroxidase/antagonistas & inibidores
Peroxidase/genética
Espécies Reativas de Oxigênio/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Enzyme Inhibitors); 0 (Reactive Oxygen Species); 5UX2SD1KE2 (Cysteamine); EC 1.11.1.7 (Peroxidase); EC 3.5.- (Amidohydrolases)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180122
[Lr] Data última revisão:
180122
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170428
[St] Status:MEDLINE


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[PMID]:28982193
[Au] Autor:Shrestha CL; Assani KD; Rinehardt H; Albastroiu F; Zhang S; Shell R; Amer AO; Schlesinger LS; Kopp BT
[Ad] Endereço:Center for Microbial Pathogenesis, The Research Institute at Nationwide Children's Hospital, Columbus, Ohio, United States of America.
[Ti] Título:Cysteamine-mediated clearance of antibiotic-resistant pathogens in human cystic fibrosis macrophages.
[So] Source:PLoS One;12(10):e0186169, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Members of the Burkholderia cepacia complex are virulent, multi-drug resistant pathogens that survive and replicate intracellularly in patients with cystic fibrosis (CF). We have discovered that B. cenocepacia cannot be cleared from CF macrophages due to defective autophagy, causing continued systemic inflammation and infection. Defective autophagy in CF is mediated through constitutive reactive oxygen species (ROS) activation of transglutaminase-2 (TG2), which causes the sequestration (accumulation) of essential autophagy initiating proteins. Cysteamine is a TG2 inhibitor and proteostasis regulator with the potential to restore autophagy. Therefore, we sought to examine the impact of cysteamine on CF macrophage autophagy and bacterial killing. Human peripheral blood monocyte-derived macrophages (MDMs) and alveolar macrophages were isolated from CF and non-CF donors. Macrophages were infected with clinical isolates of relevant CF pathogens. Cysteamine caused direct bacterial growth killing of live B. cenocepacia, B. multivorans, P. aeruginosa and MRSA in the absence of cells. Additionally, B. cenocepacia, B. multivorans, and P. aeruginosa invasion were significantly decreased in CF MDMs treated with cysteamine. Finally, cysteamine decreased TG2, p62, and beclin-1 accumulation in CF, leading to increased Burkholderia uptake into autophagosomes, increased macrophage CFTR expression, and decreased ROS and IL-1ß production. Cysteamine has direct anti-bacterial growth killing and improves human CF macrophage autophagy resulting in increased macrophage-mediated bacterial clearance, decreased inflammation, and reduced constitutive ROS production. Thus, cysteamine may be an effective adjunct to antibiotic regimens in CF.
[Mh] Termos MeSH primário: Bactérias/efeitos dos fármacos
Cisteamina/farmacologia
Fibrose Cística/microbiologia
Resistência Microbiana a Medicamentos
Macrófagos/microbiologia
[Mh] Termos MeSH secundário: Autofagia
Bactérias/crescimento & desenvolvimento
Western Blotting
Estudos de Casos e Controles
Linhagem Celular
Ensaio de Imunoadsorção Enzimática
Seres Humanos
Microscopia Confocal
Microscopia Eletrônica de Transmissão
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
5UX2SD1KE2 (Cysteamine)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171103
[Lr] Data última revisão:
171103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171006
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0186169


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[PMID]:28858751
[Au] Autor:Yuan TF; Wang ST; Li Y
[Ad] Endereço:Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan 430060, China.
[Ti] Título:Quantification of menadione from plasma and urine by a novel cysteamine-derivatization based UPLC-MS/MS method.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;1063:107-111, 2017 Sep 15.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Menadione, as the crucial component of vitamin Ks, possessed significant nutritional and clinical values. However, there was still lack of favourable quantification strategies for it to date. For improvement, a novel cysteamine derivatization based UPLC-MS/MS method was presented in this work. The derivatizating reaction was proved non-toxic, easy-handling and high-efficient, which realized the MS detection of menadione under positive mode. Benefitting from the excellent sensitivity of the derivatizating product as well as the introduction of the stable isotope dilution technique, the quantification could be achieved in the range of 0.05-50.0ng/mL for plasma and urine matrixes with satisfied accuracy and precision. After analysis of the samples from healthy volunteers after oral administration of menadione sodium bisulfite tablets, the urinary free menadione was quantified for the very first time. We believe the progress in this work could largely promote the exploration of the metabolic mechanism of vitamin K in vivo.
[Mh] Termos MeSH primário: Cromatografia Líquida de Alta Pressão/métodos
Cisteamina/química
Espectrometria de Massas em Tandem/métodos
Vitamina K 3/sangue
Vitamina K 3/urina
[Mh] Termos MeSH secundário: Seres Humanos
Modelos Lineares
Reprodutibilidade dos Testes
Sensibilidade e Especificidade
Vitamina K 3/administração & dosagem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
5UX2SD1KE2 (Cysteamine); 723JX6CXY5 (Vitamin K 3)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171002
[Lr] Data última revisão:
171002
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170901
[St] Status:MEDLINE


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[PMID]:28767736
[Au] Autor:Govindaraju VK; Bodas M; Vij N
[Ad] Endereço:College of Medicine, Central Michigan University, Mt Pleasant, Michigan, United States of America.
[Ti] Título:Cigarette smoke induced autophagy-impairment regulates AMD pathogenesis mechanisms in ARPE-19 cells.
[So] Source:PLoS One;12(8):e0182420, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Age related macular degeneration (AMD) is one of the leading causes of blindness. Genetics, environmental insult, and age-related factors all play a key role in altering proteostasis, the homeostatic process regulating protein synthesis, degradation and processing. These factors also play a role in the pathogenesis of AMD and it has been well established that cigarette smoking (CS) initiates AMD pathogenic mechanisms. The primary goal of this study is to elucidate whether CS can induce proteostasis/autophagy-impairment in retinal pigment epithelial (RPE) cells. In our preliminary analysis, it was found that cigarette smoke extract (CSE) induces accumulation of ubiquitinated proteins in the insoluble protein fraction (p < 0.01), which was subsequently mitigated through cysteamine (p < 0.01) or fisetin (p < 0.05) treatment. Further, it was verified that these CSE induced ubiquitinated proteins accumulated in the peri-nuclear spaces (p<0.05) that were cleared- off with cysteamine (p < 0.05) or fisetin (p < 0.05). Moreover, CSE-induced aggresome-formation (LC3B-GFP and Ub-RFP co-localization) and autophagy-flux impairment was significantly (p<0.01) mitigated by cysteamine (p<0.05) or fisetin (p<0.05) treatment, indicating the restoration of CSE-mediated autophagy-impairment. CSE treatment was also found to induce intracellular reactive oxygen species (ROS, p < 0.001) while impacting cell viability (p < 0.001), which was quantified using CMH2DCFDA-dye (ROS) and MTS (proliferation) or propodium iodide staining (cell viability) assays, respectively. Moreover, cysteamine and fisetin treatment ameliorated CS-mediated ROS production (p < 0.05) and diminished cell viability (p < 0.05). Lastly, CSE was found to induce cellular senescence (p < 0.001), which was significantly ameliorated by cysteamine (p < 0.001) or fisetin (p < 0.001). In conclusion, our study indicates that CS induced proteostasis/autophagy-impairment regulates mechanisms associated with AMD pathogenesis. Moreover, autophagy-inducing drugs such as cysteamine or fisetin can ameliorate AMD pathogenesis mechanisms that warrant further investigation in pre-clinical murine models.
[Mh] Termos MeSH primário: Autofagia/efeitos dos fármacos
Degeneração Macular/patologia
Fumaça/efeitos adversos
Proteínas Ubiquitinadas/metabolismo
[Mh] Termos MeSH secundário: Linhagem Celular
Núcleo Celular/metabolismo
Sobrevivência Celular/efeitos dos fármacos
Cisteamina/farmacologia
Flavonoides/farmacologia
Seres Humanos
Degeneração Macular/induzido quimicamente
Espécies Reativas de Oxigênio/metabolismo
Tabaco/efeitos adversos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Flavonoids); 0 (Reactive Oxygen Species); 0 (Smoke); 0 (Ubiquitinated Proteins); 5UX2SD1KE2 (Cysteamine); OO2ABO9578 (fisetin)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170929
[Lr] Data última revisão:
170929
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170803
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0182420


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[PMID]:28751454
[Au] Autor:Lee YJ; Jung SH; Hwang J; Jeon S; Han ET; Park WS; Hong SH; Kim YM; Ha KS
[Ad] Endereço:Department of Molecular and Cellular BiochemistryKangwon National University School of Medicine, Chuncheon, Kangwon-do, Korea.
[Ti] Título:Cysteamine prevents vascular leakage through inhibiting transglutaminase in diabetic retina.
[So] Source:J Endocrinol;235(1):39-48, 2017 Oct.
[Is] ISSN:1479-6805
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Cysteamine (an aminothiol), which is derived from coenzyme A degradation and metabolized into taurine, has beneficial effects against cystinosis and neurodegenerative diseases; however, its role in diabetic complications is unknown. Thus, we sought to determine the preventive effect of cysteamine against hyperglycemia-induced vascular leakage in the retinas of diabetic mice. Cysteamine and ethanolamine, the sulfhydryl group-free cysteamine analogue, inhibited vascular endothelial growth factor (VEGF)-induced stress fiber formation and vascular endothelial (VE)-cadherin disruption in endothelial cells, which play a critical role in modulating endothelial permeability. Intravitreal injection of the amine compounds prevented hyperglycemia-induced vascular leakage in the retinas of streptozotocin-induced diabetic mice. We then investigated the potential roles of reactive oxygen species (ROS) and transglutaminase (TGase) in the cysteamine prevention of VEGF-induced vascular leakage. Cysteamine, but not ethanolamine, inhibited VEGF-induced ROS generation in endothelial cells and diabetic retinas. In contrast, VEGF-induced TGase activation was prevented by both cysteamine and ethanolamine. Our findings suggest that cysteamine protects against vascular leakage through inhibiting VEGF-induced TGase activation rather than ROS generation in diabetic retinas.
[Mh] Termos MeSH primário: Cisteamina/administração & dosagem
Retinopatia Diabética/prevenção & controle
Retina/metabolismo
Vasos Retinianos/enzimologia
Transglutaminases/metabolismo
[Mh] Termos MeSH secundário: Animais
Retinopatia Diabética/enzimologia
Retinopatia Diabética/genética
Retinopatia Diabética/metabolismo
Seres Humanos
Masculino
Camundongos
Camundongos Endogâmicos C57BL
Espécies Reativas de Oxigênio/metabolismo
Retina/efeitos dos fármacos
Retina/enzimologia
Vasos Retinianos/efeitos dos fármacos
Transglutaminases/genética
Fator A de Crescimento do Endotélio Vascular/genética
Fator A de Crescimento do Endotélio Vascular/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Reactive Oxygen Species); 0 (Vascular Endothelial Growth Factor A); 5UX2SD1KE2 (Cysteamine); EC 2.3.2.13 (Transglutaminases)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170918
[Lr] Data última revisão:
170918
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170729
[St] Status:MEDLINE
[do] DOI:10.1530/JOE-17-0109


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[PMID]:28576495
[Au] Autor:Yamashita N; Yashiro M; Ogawa H; Namba H; Nosaka N; Fujii Y; Morishima T; Tsukahara H; Yamada M
[Ad] Endereço:Department of Virology, Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University, Okayama, Japan; Department of Pediatrics, Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University, Okayama, Japan. Electronic address: noyamash@okayama-u.a
[Ti] Título:Metabolic pathway catalyzed by Vanin-1 pantetheinase plays a suppressive role in influenza virus replication in human alveolar epithelial A549 cells.
[So] Source:Biochem Biophys Res Commun;489(4):466-471, 2017 Aug 05.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Our previous analysis of gene expression profiles in the peripheral blood from patients with influenza A (H1N1) pdm09 pneumonia revealed elevated transcription levels of the vanin-1 (vascular non-inflammatory molecule 1, VNN1) gene, which encodes an epithelial ectoenzyme with pantetheinase activity involved in recycling coenzyme A. Here, to elucidate the role of VNN1 in influenza A virus (IAV) H1N1 infection, we investigated the change of VNN1 expression in the context of IAV infection and the effects of its related substances, i.e., its direct substrate pantetheine and its two metabolites pantothenic acid and cysteamine on the replication of IAV in the human alveolar epithelial carcinoma cell line A549. The messenger RNA expression of VNN1 in A549 cells was significantly increased (by 4.9-fold) after IAV infection under an elevated concentration of pantetheine. Moreover, VNN1 mRNA levels were elevated by > 100-fold in response to pro-inflammatory cytokines, especially TNF-α and IL-1ß. Pantetheine significantly reduced the IAV replication and IAV Matrix 1 (M1) mRNA levels when it was administered prior to and during infection. In addition, cysteamine treatment during IAV infection significantly reduced the viral replication and IAV M1 mRNA levels, whereas pantothenic acid did not. These findings suggest that the metabolic pathway catalyzed by VNN1 pantetheinase plays a suppressive role in IAV infection in the respiratory tract, especially in severe conditions under hypercytokinemia.
[Mh] Termos MeSH primário: Amidoidrolases/metabolismo
Vírus da Influenza A/metabolismo
Replicação Viral
[Mh] Termos MeSH secundário: Amidoidrolases/genética
Biocatálise
Linhagem Celular Tumoral
Cisteamina/farmacologia
Citocinas/metabolismo
Relação Dose-Resposta a Droga
Proteínas Ligadas por GPI/genética
Proteínas Ligadas por GPI/metabolismo
Seres Humanos
Vírus da Influenza A/efeitos dos fármacos
Ácido Pantotênico/farmacologia
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
Relação Estrutura-Atividade
Regulação para Cima
Replicação Viral/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cytokines); 0 (GPI-Linked Proteins); 0 (RNA, Messenger); 19F5HK2737 (Pantothenic Acid); 5UX2SD1KE2 (Cysteamine); EC 3.5.- (Amidohydrolases); EC 3.5.1.- (pantetheinase)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170604
[St] Status:MEDLINE


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[PMID]:28475934
[Au] Autor:Matos ALL; Pereira G; Cabral Filho PE; Santos BS; Fontes A
[Ad] Endereço:Departamento de Biofísica e Radiobiologia, Universidade Federal de Pernambuco, 50670-901 Recife, Brazil.
[Ti] Título:Delivery of cationic quantum dots using fusogenic liposomes in living cells.
[So] Source:J Photochem Photobiol B;171:43-49, 2017 Jun.
[Is] ISSN:1873-2682
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Quantum dots (QDs) are fluorescent nanocrystals that present unique optical properties, especially a high photostability. However, their use for intracellular studies is still limited since their passage through the living cell membranes does not occur passively. In this work, we adapted the ethanol injection method to encapsulate cationic hydrophilic QDs into fusogenic liposomes, to deliver them in living cells. Liposomes were characterized using zeta potential, dynamic light scattering (DLS), fluorescence microscopy and transmission electron microscopy (TEM). Red blood cells (RBCs) were applied as models in this study to probe the liposome fusion with the cell membrane since RBCs do not present endocytic activity. Therefore, HeLa cells were also applied to test the QDs delivery by the liposomes. The TEM and the fluorescence microscopy confirmed the QDs encapsulation, with an efficiency of 43%, determined by UV-vis spectroscopy. Zeta potential showed that the QDs-loaded fusogenic liposomes were positively charged and presented an average size of 343nm, determined by DLS. Furthermore, fluorescence microscopy analyses of RBCs and HeLa cells confirmed the liposomes fusion with the cell membrane and suggested the release of QDs into cells. Thus, we expect that this work will contribute to improve the use of QDs as fluorescent probes to intracellular studies.
[Mh] Termos MeSH primário: Lipossomos/química
Pontos Quânticos/química
[Mh] Termos MeSH secundário: Compostos de Cádmio/química
Cátions/química
Cisteamina/química
Difusão Dinâmica da Luz
Eritrócitos/citologia
Eritrócitos/metabolismo
Células HeLa
Seres Humanos
Microscopia Eletrônica de Transmissão
Microscopia de Fluorescência
Pontos Quânticos/metabolismo
Telúrio/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cadmium Compounds); 0 (Cations); 0 (Liposomes); 5UX2SD1KE2 (Cysteamine); NQA0O090ZJ (Tellurium); STG188WO13 (cadmium telluride)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170920
[Lr] Data última revisão:
170920
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170506
[St] Status:MEDLINE


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[PMID]:28426870
[Au] Autor:Liang H; Labbé A; Le Mouhaër J; Plisson C; Baudouin C
[Ad] Endereço:Department of Ophthalmology III, Départment Hospitalo-Universitaire View Maintain, INSERM-DHOS, and Center of Clinical Investigations 1423, Quinze-Vingts National Ophthalmology Hospital, Institut de la Vision, Université Pierre-et-Marie-Curie University Paris 06, Paris, France.
[Ti] Título:A New Viscous Cysteamine Eye Drops Treatment for Ophthalmic Cystinosis: An Open-Label Randomized Comparative Phase III Pivotal Study.
[So] Source:Invest Ophthalmol Vis Sci;58(4):2275-2283, 2017 Apr 01.
[Is] ISSN:1552-5783
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Purpose: The purpose of this study was to evaluate the efficacy of new viscous cysteamine hydrochloride (CH) eye drops (vCH 0.55%) compared with standard CH 0.10% drops treatment. Methods: This was an open-label, phase III, randomized, two-arm multicenter trial conducted at two centers in France. Cystinosis patients ≥2 years old were randomized 1:1 to receive eye drops, four times per day for 90 days in both eyes. We compared the superiority in reducing corneal cystine crystal density as assessed by in vivo confocal microscopy (IVCM). We also evaluated photophobia, corneal cystine crystal scores (CCCSs), and cystine crystal depth measured by optical coherence tomography. Safety objectives were to assess adverse events (AEs), local adverse drug reactions, and ocular safety parameters. Results: We included 15 patients with vCH 0.55% and 16 patients with CH 0.10% drops for 90 days. The mean absolute change in IVCM total score at day 90 in the vCH 0.55% drops group (-4.6 ± 3.1) was significantly greater than and superior to the mean absolute change in the CH 0.10% drops group (-0.46 ± 3.38; P < 0.0001). Photophobia, CCCS, and corneal cystine crystal depth were significantly more improved in the vCH 0.55% drops group than in the CH 0.10% group. The most frequent local adverse drug reactions in both groups were stinging, burning, redness, and blurred vision. Conclusions: vCH 0.55% was effective in reducing corneal cystine crystal density and superior to treatment with CH 0.10% drops, which offer advantages over hospital pharmacy formulations and is a more preferable and convenient treatment option.
[Mh] Termos MeSH primário: Córnea/metabolismo
Doenças da Córnea/tratamento farmacológico
Cisteamina/administração & dosagem
Cistina/metabolismo
Cistinose/tratamento farmacológico
[Mh] Termos MeSH secundário: Adolescente
Córnea/patologia
Doenças da Córnea/diagnóstico
Doenças da Córnea/metabolismo
Cistina/efeitos dos fármacos
Eliminadores de Cistina/administração & dosagem
Cistinose/diagnóstico
Cistinose/metabolismo
Relação Dose-Resposta a Droga
Feminino
Seguimentos
Seres Humanos
Masculino
Microscopia Confocal
Soluções Oftálmicas
Estudos Retrospectivos
Tomografia de Coerência Óptica
Resultado do Tratamento
Acuidade Visual
Adulto Jovem
[Pt] Tipo de publicação:CLINICAL TRIAL, PHASE III; JOURNAL ARTICLE; MULTICENTER STUDY; RANDOMIZED CONTROLLED TRIAL
[Nm] Nome de substância:
0 (Cystine Depleting Agents); 0 (Ophthalmic Solutions); 48TCX9A1VT (Cystine); 5UX2SD1KE2 (Cysteamine)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170719
[Lr] Data última revisão:
170719
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170421
[St] Status:MEDLINE
[do] DOI:10.1167/iovs.16-21080


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[PMID]:28239795
[Au] Autor:Zhukova OS; Smirnova ZS; Chikileva IO; Kiselevskii MV
[Ad] Endereço:Laboratory of Cell Immunity, Laboratory of Experimental Cheotherapy, N. N. Blokhin Cancer Research Center, Russian Ministry of Health, Moscow, Russia. msvz@yandex.ru.
[Ti] Título:Antiproliferative Activity of a New Nitrosyl Iron Complex with Cysteamine in Human Tumor Cells In Vitro.
[So] Source:Bull Exp Biol Med;162(4):583-588, 2017 Feb.
[Is] ISSN:1573-8221
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We studied cytotoxic activity of a new NO-releasing tetranitrosyl binuclear iron complex with cysteamine (CysAm) for human tumor cells, the relationship between the expression of O6-methylguanine-DNA methyltransferase (MGMT) and cell sensitivity to CysAm, and apoptosis-inducing capacity of this preparation. It was found that histogenetically different cell lines are characterized by different sensitivity to CysAm, and this parameter correlated with the basal level of MGMT. CysAm induced apoptosis via activation of caspases 3 and 7. These data suggest that CysAm can be considered as a potential antitumor agent, but definitive conclusions can be made after preclinical trials.
[Mh] Termos MeSH primário: Antineoplásicos/farmacologia
Proliferação Celular/efeitos dos fármacos
Complexos de Coordenação/farmacologia
Cisteamina/química
Metilases de Modificação do DNA/antagonistas & inibidores
Enzimas Reparadoras do DNA/antagonistas & inibidores
Ferro/química
Óxidos de Nitrogênio/química
Proteínas Supressoras de Tumor/antagonistas & inibidores
[Mh] Termos MeSH secundário: Células A549
Antineoplásicos/síntese química
Apoptose/efeitos dos fármacos
Linhagem Celular Tumoral
Sobrevivência Celular/efeitos dos fármacos
Complexos de Coordenação/síntese química
Metilases de Modificação do DNA/genética
Metilases de Modificação do DNA/metabolismo
Reparo do DNA/efeitos dos fármacos
Enzimas Reparadoras do DNA/genética
Enzimas Reparadoras do DNA/metabolismo
Relação Dose-Resposta a Droga
Expressão Gênica
Seres Humanos
Células K562
Especificidade de Órgãos
Proteínas Supressoras de Tumor/genética
Proteínas Supressoras de Tumor/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (Coordination Complexes); 0 (Nitrogen Oxides); 0 (Tumor Suppressor Proteins); 5UX2SD1KE2 (Cysteamine); 68586-27-6 (dinitrosyl iron complex); E1UOL152H7 (Iron); EC 2.1.1.- (DNA Modification Methylases); EC 2.1.1.63 (MGMT protein, human); EC 6.5.1.- (DNA Repair Enzymes)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170315
[Lr] Data última revisão:
170315
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170228
[St] Status:MEDLINE
[do] DOI:10.1007/s10517-017-3663-8



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