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[PMID]:29325850
[Au] Autor:Nejat Dehkordi M; Akerman B
[Ad] Endereço:Department of Basic Science, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran; Department of Chemical and Biological Engineering, Chalmers University of Technology, SE-412 96, Gothenburg, Sweden. Electronic address: m.nejat@iaushk.ac.ir.
[Ti] Título:Interaction of DNA with water soluble complex of Nickle and formation of DNA cross-links.
[So] Source:Chem Biol Interact;282:55-62, 2018 Feb 25.
[Is] ISSN:1872-7786
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:Interaction of double stranded DNA with bulky and hydrophobic Salen type Schiff base complex: [N, N' Bis [3- tert-butyl-5-[triphenyl-phosphonium - methyl] - salicylidene] 1,2 ethylene-diamine nickel(III) acetate (refer to Ni Salen complex) was extensively investigated using the spectroscopic techniques and gel electrophoresis. Absorption titration experiment showed the hypochromic effect and the significant red shift of the complex absorption. In competition experiments with ethidium bromide (EB), Ni Salen complex exhibited non-competitive binding at high concentrations. UV-vis absorption and fluorescence emission data agreed on a binding constant of (1.64 ±â€¯0.01) µM , thereby showing the strong interaction of the complex with DNA; also, a binding site size of 2.33 ±â€¯0.01 base pairs per complex was achieved. Thermal denaturation experiment showed that T of calf thymus-DNA was increased by approximately 10 °C at a molar ratio of the dye/base of 0.2. The CD spectra of DNA exhibited an increase in both positive and negative peaks without any shift in the position of bands upon addition of the complex. The amplitude of the LD spectra of DNA was decreased in the presence of the complex. Reduced linear dichroism (LD ) revealed that the transition moment of complex was parallel to the DNA helix axis. Gel electrophoresis experiments confirmed that Ni Salen complex had no nuclease/DNA cleaving activity; also, DNA-DNA cross links were formed at high concentrations of complex, leading to the aggregation of DNA.
[Mh] Termos MeSH primário: DNA/química
Níquel/química
RNA de Cadeia Dupla/química
Água/química
[Mh] Termos MeSH secundário: Animais
Sítios de Ligação
Bovinos
Eletroforese em Gel de Ágar/métodos
Etídio/química
Etilenodiaminas/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Ethylenediamines); 0 (RNA, Double-Stranded); 059QF0KO0R (Water); 7OV03QG267 (Nickel); 9007-49-2 (DNA); 91080-16-9 (calf thymus DNA); 94-93-9 (disalicylaldehyde ethylenediamine); EN464416SI (Ethidium)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180209
[Lr] Data última revisão:
180209
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180113
[St] Status:MEDLINE


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[PMID]:27770415
[Au] Autor:Knezevic-Jugovic ZD; Grbavcic SZ; Jovanovic JR; Stefanovic AB; Bezbradica DI; Mijin DZ; Antov MG
[Ad] Endereço:Department for Biochemical Engineering and Biotechnology, Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, Belgrade, Serbia. zknez@tmf.bg.ac.rs.
[Ti] Título:Covalent Immobilization of Enzymes on Eupergit Supports: Effect of the Immobilization Protocol.
[So] Source:Methods Mol Biol;1504:75-91, 2017.
[Is] ISSN:1940-6029
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A selection of best combination of adequate immobilization support and efficient immobilization method is still a key requirement for successful application of immobilized enzymes on an industrial level. Eupergit supports exhibit good mechanical and chemical properties and allow establishment of satisfactory hydrodynamic regime in enzyme reactors. This is advantageous for their wide application in enzyme immobilization after finding the most favorable immobilization method. Methods for enzyme immobilization that have been previously reported as efficient considering the obtained activity of immobilized enzyme are presented: direct binding to polymers via their epoxy groups, binding to polymers via a spacer made from ethylene diamine/glutaraldehyde, and coupling the periodate-oxidized sugar moieties of the enzymes to the polymer beads. The modification of the conventionally immobilized enzyme with ethylenediamine via the carbodiimide route seems to be a powerful tool to improve its stability and catalytic activity.
[Mh] Termos MeSH primário: Candida/enzimologia
Enzimas Imobilizadas/química
Compostos de Epóxi/química
Etilenodiaminas/química
Glutaral/química
Lipase/química
Polímeros/química
[Mh] Termos MeSH secundário: Acrilamidas/química
Aminação
Candida/química
Candida/metabolismo
Estabilidade Enzimática
Enzimas Imobilizadas/metabolismo
Lipase/metabolismo
Metacrilatos/química
Oxirredução
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acrylamides); 0 (Enzymes, Immobilized); 0 (Epoxy Compounds); 0 (Ethylenediamines); 0 (Methacrylates); 0 (Polymers); 60V9STC53F (ethylenediamine); 93356-75-3 (Eupergit C); EC 3.1.1.3 (Lipase); K67NG89J77 (methacrylamide); T3C89M417N (Glutaral)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180115
[Lr] Data última revisão:
180115
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161023
[St] Status:MEDLINE


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[PMID]:28452034
[Au] Autor:Fang L; Wang M; Cai L; Cang L
[Ad] Endereço:State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, Northwest A&F University, Yangling, 712100, China.
[Ti] Título:Deciphering biodegradable chelant-enhanced phytoremediation through microbes and nitrogen transformation in contaminated soils.
[So] Source:Environ Sci Pollut Res Int;24(17):14627-14636, 2017 Jun.
[Is] ISSN:1614-7499
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Biodegradable chelant-enhanced phytoremediation offers an alternative treatment technique for metal contaminated soils, but most studies to date have addressed on phytoextraction efficiency rather than comprehensive understanding of the interactions among plant, soil microbes, and biodegradable chelants. In the present study, we investigated the impacts of biodegradable chelants, including nitrilotriacetate, S,S-ethylenediaminedisuccinic acid (EDDS), and citric acid on soil microbes, nitrogen transformation, and metal removal from contaminated soils. The EDDS addition to soil showed the strongest ability to promote the nitrogen cycling in soil, ryegrass tissue, and microbial metabolism in comparison with other chelants. Both bacterial community-level physiological profiles and soil mass specific heat rates demonstrated that soil microbial activity was inhibited after the EDDS application (between day 2 and 10), but this effect completely vanished on day 30, indicating the revitalization of microbial activity and community structure in the soil system. The results of quantitative real-time PCR revealed that the EDDS application stimulated denitrification in soil by increasing nitrite reductase genes, especially nirS. These new findings demonstrated that the nitrogen release capacity of biodegradable chelants plays an important role in accelerating nitrogen transformation, enhancing soil microbial structure and activity, and improving phytoextraction efficiency in contaminated soil.
[Mh] Termos MeSH primário: Biodegradação Ambiental
Quelantes
Etilenodiaminas
Nitrogênio/metabolismo
Poluentes do Solo/metabolismo
[Mh] Termos MeSH secundário: Solo
Succinatos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Chelating Agents); 0 (Ethylenediamines); 0 (Soil); 0 (Soil Pollutants); 0 (Succinates); N762921K75 (Nitrogen)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171215
[Lr] Data última revisão:
171215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE
[do] DOI:10.1007/s11356-017-9029-y


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[PMID]:28964927
[Au] Autor:Zhong YH; Dhawan J; Kovoor JA; Sullivan J; Zhang WX; Choi D; Biegon A
[Ad] Endereço:Department of Neurology, the First Affiliated Hospital of Sun Yat-Sen University, No. 58, Zhongshan Road II, Guangzhou, Guangdong Province 510080, PR China; Department of Neurology, Stony Brook University, 100 Nicolls Road, Stony Brook, NY 11794-2565, USA. Electronic address: zhongbinyue@163.com.
[Ti] Título:Aromatase and neuroinflammation in rat focal brain ischemia.
[So] Source:J Steroid Biochem Mol Biol;174:225-233, 2017 Nov.
[Is] ISSN:1879-1220
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Accumulating evidence suggests that expression of aromatase, the enzyme responsible for the conversion of androgens to estrogens, is transiently upregulated in rat stroke models. It was further suggested that increased aromatase expression is linked to neuroinflammation and that it is neuroprotective in females. Our goal was to investigate aromatase upregulation in male rats subjected to experimental stroke in relationship to neuroinflammation, infarct and response to treatment with different putative neuroprotective agents. Intact male rats were subjected to transient (90min) middle cerebral artery occlusion (MCAO) and administered selfotel (N-methyl-d-aspartic acid (NMDA) receptor competitive antagonist), TPEN (a zinc chelator), a combination of the two drugs or vehicle, injected immediately after reperfusion. Animals were killed 14days after MCAO and consecutive brain sections used to measure aromatase expression, cerebral infarct volume and neuroinflammation. Quantitative immunohistochemistry (IHC) demonstrated increased brain aromatase expression in the peri-infarct area relative to contralesional area, which was partially abrogated by neuroprotective agents. There was no correlation between aromatase expression in the peri-infarct zone and infarct volume, which was reduced by neuroprotective agents. Microglial activation, measured by quantitative autoradiography, was positively correlated with infarct and inversely correlated with aromatase expression in the peri-infarct zone. Our findings indicate that focal ischemia upregulates brain aromatase in the male rat brain at 14days post surgery, which is within the time frame documented in females. However, the lack of negative correlation between aromatase expression and infarct volume and lack of positive correlation between microgliosis and aromatase do not support a major role for aromatase as a mediator of neuroprotection or a causal relationship between microglial activation and increased aromatase expression in male focal ischemia.
[Mh] Termos MeSH primário: Aromatase/metabolismo
Encéfalo/metabolismo
Encéfalo/patologia
Infarto da Artéria Cerebral Média/metabolismo
Infarto da Artéria Cerebral Média/patologia
[Mh] Termos MeSH secundário: Animais
Encéfalo/efeitos dos fármacos
Quelantes/farmacologia
Etilenodiaminas/farmacologia
Antagonistas de Aminoácidos Excitatórios/farmacologia
Seres Humanos
Isoquinolinas/farmacologia
Masculino
Ácidos Pipecólicos/farmacologia
Ratos
Ratos Long-Evans
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Chelating Agents); 0 (Ethylenediamines); 0 (Excitatory Amino Acid Antagonists); 0 (Isoquinolines); 0 (Pipecolic Acids); 4VGJ4A41L2 (selfotel); EC 1.14.14.1 (Aromatase); R9PTU1U29I (N,N,N',N'-tetrakis(2-pyridylmethyl)ethylenediamine); YNF83VN1RL (PK 11195)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171113
[Lr] Data última revisão:
171113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171002
[St] Status:MEDLINE


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[PMID]:28944496
[Au] Autor:Nesbitt H; Worthington J; Errington RJ; Patterson LH; Smith PJ; McKeown SR; McKenna DJ
[Ad] Endereço:Biomedical Sciences, Research Institute, University of Ulster, Cromore Road, Coleraine, Northern Ireland, United Kingdom.
[Ti] Título:The unidirectional hypoxia-activated prodrug OCT1002 inhibits growth and vascular development in castrate-resistant prostate tumors.
[So] Source:Prostate;77(15):1539-1547, 2017 Nov.
[Is] ISSN:1097-0045
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: OCT1002 is a unidirectional hypoxia-activated prodrug (uHAP) OCT1002 that can target hypoxic tumor cells. Hypoxia is a common feature in prostate tumors and is known to drive disease progression and metastasis. It is, therefore, a rational therapeutic strategy to directly target hypoxic tumor cells in an attempt to improve treatment for this disease. Here we tested OCT1002 alone and in combination with standard-of-care agents in hypoxic models of castrate-resistant prostate cancer (CRPC). METHODS: The effect of OCT1002 on tumor growth and vasculature was measured using murine PC3 xenograft and dorsal skin fold (DSF) window chamber models. The effects of abiraterone, docetaxel, and cabazitaxel, both singly and in combination with OCT1002, were also compared. RESULTS: The hypoxia-targeting ability of OCT1002 effectively controls PC3 tumor growth. The effect was evident for at least 42 days after exposure to a single dose (30 mg/kg) and was comparable to, or better than, drugs currently used in the clinic. In DSF experiments OCT1002 caused vascular collapse in the PC3 tumors and inhibited the revascularization seen in controls. In this model OCT1002 also enhanced the anti-tumor effects of abiraterone, cabazitaxel, and docetaxel; an effect which was accompanied by a more prolonged reduction in tumor vasculature density. CONCLUSIONS: These studies provide the first evidence that OCT1002 can be an effective agent in treating hypoxic, castrate-resistant prostate tumors, either singly or in combination with established chemotherapeutics for prostate cancer.
[Mh] Termos MeSH primário: Antraquinonas/farmacologia
Etilenodiaminas/farmacologia
Pró-Fármacos/farmacologia
Neoplasias de Próstata Resistentes à Castração/tratamento farmacológico
Neoplasias de Próstata Resistentes à Castração/metabolismo
[Mh] Termos MeSH secundário: Animais
Antraquinonas/farmacocinética
Processos de Crescimento Celular/efeitos dos fármacos
Hipóxia Celular/fisiologia
Linhagem Celular Tumoral
Etilenodiaminas/farmacocinética
Seres Humanos
Masculino
Camundongos
Camundongos Nus
Pró-Fármacos/farmacocinética
Neoplasias de Próstata Resistentes à Castração/irrigação sanguínea
Neoplasias de Próstata Resistentes à Castração/patologia
Ensaios Antitumorais Modelo de Xenoenxerto
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anthraquinones); 0 (Ethylenediamines); 0 (OCT1002); 0 (Prodrugs)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171106
[Lr] Data última revisão:
171106
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170926
[St] Status:MEDLINE
[do] DOI:10.1002/pros.23434


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[PMID]:28806652
[Au] Autor:Race M
[Ad] Endereço:Dipartimento di Ingegneria Civile, Edile ed Ambientale, Università di Napoli Federico II, 80125 Napoli, Italy. Electronic address: marco.race@unina.it.
[Ti] Título:Applicability of alkaline precipitation for the recovery of EDDS spent solution.
[So] Source:J Environ Manage;203(Pt 1):358-363, 2017 Dec 01.
[Is] ISSN:1095-8630
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:This paper presents an innovative procedure for the recovery of SS-ethylenediamine-N,N'-disuccinic acid (EDDS) solution used for soil washing processes. The procedure is derived from that applied for the recovery of ethylenediamine-tetraacetic acid (EDTA), modifying and optimizing the choice of the chemical agents used for the protonation of the chelant, the exchange of the metals, and the final precipitation of the unwanted compounds. To select the reagents and test the proposed approach, an experimental study was conducted on real EDDS spent solutions, obtained washing a Cu and Zn real contaminated soil. According to the results obtained, the precipitation ranges from 30% to more than 90% for both Cu and Zn, depending on the adopted reagent sequence, and on the molar ratio between the salt and the chelant contained in the spent solution. Data were in agreement with chemical equilibrium predicted in ideal conditions. The recovered solutions had a reduced ability to remove the contaminants when applied in a new soil washing cycle (15% less for Cu and 30% less for Zn) because of the high concentration of alkaline metal ions required for the precipitation. At the same time, they were more biodegradable compared to non-treated solutions, confirming that EDDS-metal chelates may represent a threat for biological wastewater processes.
[Mh] Termos MeSH primário: Etilenodiaminas/química
Poluentes do Solo
Succinatos/química
[Mh] Termos MeSH secundário: Quelantes
Ácido Edético
Metais Pesados
Águas Residuais
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Chelating Agents); 0 (Ethylenediamines); 0 (Metals, Heavy); 0 (Soil Pollutants); 0 (Succinates); 0 (Waste Water); 20846-91-7 (N,N'-ethylenediamine disuccinic acid); 9G34HU7RV0 (Edetic Acid)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171113
[Lr] Data última revisão:
171113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170815
[St] Status:MEDLINE


  7 / 3632 MEDLINE  
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[PMID]:28735861
[Au] Autor:Domazetovic V; Fontani F; Tanini D; D'Esopo V; Viglianisi C; Marcucci G; Panzella L; Napolitano A; Brandi ML; Capperucci A; Menichetti S; Vincenzini MT; Iantomasi T
[Ad] Endereço:Department of Biomedical, Experimental and Clinical Sciences "Mario Serio", University of Florence, Viale Morgagni 50, I-50134 Florence, Italy.
[Ti] Título:Protective role of benzoselenophene derivatives of resveratrol on the induced oxidative stress in intestinal myofibroblasts and osteocytes.
[So] Source:Chem Biol Interact;275:13-21, 2017 Sep 25.
[Is] ISSN:1872-7786
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:Resveratrol (RE), a polyphenolic compound present in some food and plants, is characterized by anti-inflammatory and antioxidant properties. However, it is quickly metabolized with consequent loss of its efficacy. In this study, the antioxidant effect of 2-phenyl-benzoselenophene derivatives (VD0, VD1 and VD2) was detected in intestinal myofibroblast and osteocyte cell lines in which the oxidative stress was induced by GSH depletion or starvation, respectively. In fact, the oxidative stress is involved in pathogenesis of inflammatory bowel diseases (IBD) and in increased osteoclastogenesis in osteoporosis. Our results show that these derivatives have major antioxidant power in reducing and/or restoring radical oxygen species to control values than RE itself in both cell types. Moreover, derivatives have different antioxidant capacity in myofibroblasts and in osteocytes and this can be due to different degree of oxidative stress and structural characteristics of these compounds. Some of the synthesized RE analogs have shown anti-bacterial role in IBD and anti-resorptive activity in bone pathologies related to inflammatory and osteoporotic processes. Thus, we suggest benzoselenophene derivatives as good candidates for alternative therapy and/or therapeutic support in these pathologies.
[Mh] Termos MeSH primário: Etilenodiaminas/química
Miofibroblastos/efeitos dos fármacos
Osteócitos/efeitos dos fármacos
Estresse Oxidativo/efeitos dos fármacos
Substâncias Protetoras/farmacologia
Estilbenos/química
[Mh] Termos MeSH secundário: Linhagem Celular
Sobrevivência Celular/efeitos dos fármacos
Etilenodiaminas/farmacologia
Seres Humanos
Intestinos/citologia
Miofibroblastos/citologia
Miofibroblastos/metabolismo
Osteócitos/citologia
Osteócitos/metabolismo
Substâncias Protetoras/química
Espécies Reativas de Oxigênio/metabolismo
Estilbenos/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Ethylenediamines); 0 (Protective Agents); 0 (Reactive Oxygen Species); 0 (Stilbenes); Q369O8926L (resveratrol)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170725
[St] Status:MEDLINE


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[PMID]:28646396
[Au] Autor:Yan L; Li C; Zhang J; Moodley O; Liu S; Lan C; Gao Q; Zhang W
[Ad] Endereço:College of Resource and Environmental Science, Jilin Agricultural University, Changchun, 130118, China.
[Ti] Título:Enhanced Phytoextraction of Lead from Artificially Contaminated Soil by Mirabilis jalapa with Chelating Agents.
[So] Source:Bull Environ Contam Toxicol;99(2):208-212, 2017 Aug.
[Is] ISSN:1432-0800
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A microcosm experiment was conducted to compare how different chelating agents enhance the phytoextraction of lead (Pb) by Mirabilis jalapa from an artificially Pb-contaminated soil. Chelating agents used included ethylene diamine tetraacetic acid (EDTA), nitrilotriacetic acid (NTA), ethylene diamine disuccinic acid (EDDS), oxalic acid, humic acid, citric acid and tartaric acid, with concentrations ranging from 0 to 2000 mg/kg. Results show that with increasing concentrations of chelating agents, the concentrations, bioaccumulation coefficients, and translocation factors of Pb in M. jalapa gradually increased. Of all chelating agents used, the concentrations, bioaccumulation coefficients and translocation factors of Pb in M. jalapa were higher for EDTA and NTA than for the other chelating agents. Given the potential environmental risks of EDTA, we recommend NTA be used as a suitable chelating agent to increase the phytoremediation of Pb-contaminated soil with M. jalapa.
[Mh] Termos MeSH primário: Quelantes/química
Recuperação e Remediação Ambiental/métodos
Chumbo/metabolismo
Mirabilis/fisiologia
Poluentes do Solo/metabolismo
[Mh] Termos MeSH secundário: Biodegradação Ambiental
Ácido Edético
Etilenodiaminas
Chumbo/análise
Solo
Poluentes do Solo/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Chelating Agents); 0 (Ethylenediamines); 0 (Soil); 0 (Soil Pollutants); 2P299V784P (Lead); 60V9STC53F (ethylenediamine); 9G34HU7RV0 (Edetic Acid)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170625
[St] Status:MEDLINE
[do] DOI:10.1007/s00128-017-2127-1


  9 / 3632 MEDLINE  
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[PMID]:28525360
[Au] Autor:Song T; Zhao J; Ma X; Zhang Z; Jiang B; Yang Y
[Ti] Título:Role of sigma 1 receptor in high fat diet-induced peripheral neuropathy.
[So] Source:Biol Chem;398(10):1141-1149, 2017 Sep 26.
[Is] ISSN:1437-4315
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:The neurobiological mechanisms of obesity-induced peripheral neuropathy are poorly understood. We evaluated the role of Sigma-1 receptor (Sig-1R) and NMDA receptor (NMDARs) in the spinal cord in peripheral neuropathy using an animal model of high fat diet-induced diabetes. We examined the expression of Sig-1R and NMDAR subunits GluN2A and GluN2B along with postsynaptic density protein 95 (PSD-95) in the spinal cord after 24-week HFD treatment in both wild-type and Sig-1R-/- mice. Finally, we examined the effects of repeated intrathecal administrations of selective Sig-1R antagonists BD1047 in HFD-fed wild-type mice on peripheral neuropathy. Wild-type mice developed tactile allodynia and thermal hypoalgesia after 24-week HFD treatment. HFD-induced peripheral neuropathy correlated with increased expression of GluN2A and GluN2B subunits of NMDARs, PDS-95, and Sig-1R, as well as increased Sig-1R-NMDAR interaction in the spinal cord. In contrast, Sig-1R-/- mice did not develop thermal hypoalgesia or tactile allodynia after 24-week HFD treatment, and the levels of GluN2A, GluN2B, and PSD-95 were not altered in the spinal cord of HFD-fed Sig-1R-/- mice. Finally, repeated intrathecal administrations of selective Sig-1R antagonists BD1047 in HFD-fed wild-type mice attenuated peripheral neuropathy. Our results suggest that obesity-associated peripheral neuropathy may involve Sig-1R-mediated enhancement of NMDAR expression in the spinal cord.
[Mh] Termos MeSH primário: Dieta Hiperlipídica/efeitos adversos
Doenças do Sistema Nervoso Periférico/metabolismo
Receptores sigma/metabolismo
[Mh] Termos MeSH secundário: Animais
Etilenodiaminas/administração & dosagem
Etilenodiaminas/farmacologia
Injeções Espinhais
Masculino
Camundongos
Camundongos Endogâmicos C57BL
Camundongos Knockout
Doenças do Sistema Nervoso Periférico/patologia
Receptores sigma/antagonistas & inibidores
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Ethylenediamines); 0 (Receptors, sigma); 0 (sigma-1 receptor); 1S3X75QGDO (N-(2-(3,4-Dichlorphenyl)ethyl)-N,N',N'-trimethyl-1,2-ethandiamin)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170520
[St] Status:MEDLINE


  10 / 3632 MEDLINE  
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[PMID]:28494356
[Au] Autor:Sidhu GPS; Singh HP; Batish DR; Kohli RK
[Ad] Endereço:Department of Environment Studies, Panjab University, Chandigarh 160014, India. Electronic address: gagan1986sidhu@gmail.com.
[Ti] Título:Appraising the role of environment friendly chelants in alleviating lead by Coronopus didymus from Pb-contaminated soils.
[So] Source:Chemosphere;182:129-136, 2017 Sep.
[Is] ISSN:1879-1298
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In a screenhouse experiment, we investigated the role of two environment friendly chelants, Ammonium molybdate and EDDS for Pb mobilisation and its extraction by Coronopus didymus under completely randomized controlled conditions. Seedlings of C. didymus were grown in pots having Pb-contaminated soil (1200 and 2200 mg kg ) for 6 weeks. Plants were harvested, 1 week after the addition of A. molybdate and EDDS. Results revealed that A. molybdate and EDDS enhanced the uptake and accumulation of Pb in roots and shoots of C. didymus. At 2200 mg kg Pb level, compared to Pb-alone treatment, the maximal concentration of Pb was increased upto ∼10% and ∼19%, in roots whereas ∼8% and ∼18%, respectively, in shoots on addition of 2 mmol kg A. molybdate and EDDS. Additionally, Pb + EDDS treatments enhanced the plant biomass and triggered strong antioxidative response, more efficaciously than Pb + A. molybdate and Pb-alone treated plants. In this study, EDDS relative to A. molybdate was more efficient in mobilising and extracting Pb from soil. Although, EDDS followed by A. molybdate had good efficacy in mitigating Pb from contaminated soils but C. didymus itself has the inherent affinity to tolerate and accumulate Pb from contaminated soils and hence in future, can be used either alone or with some other eco-friendly amendments for soil remediation purposes.
[Mh] Termos MeSH primário: Biodegradação Ambiental
Chumbo/farmacocinética
[Mh] Termos MeSH secundário: Etilenodiaminas/química
Chumbo/isolamento & purificação
Molibdênio/química
Raízes de Plantas/química
Raízes de Plantas/metabolismo
Poluentes do Solo/análise
Poluentes do Solo/isolamento & purificação
Poluentes do Solo/farmacocinética
Succinatos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Ethylenediamines); 0 (Soil Pollutants); 0 (Succinates); 2P299V784P (Lead); 81AH48963U (Molybdenum); OGI154X474 (ammonium molybdate)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170512
[St] Status:MEDLINE



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