Base de dados : MEDLINE
Pesquisa : D02.092.782.258.368.250 [Categoria DeCS]
Referências encontradas : 23559 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 2356 ir para página                         

  1 / 23559 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29175400
[Au] Autor:Kaurin A; Cernilogar Z; Lestan D
[Ad] Endereço:University of Ljubljana, Biotechnical Faculty, Agronomy Department, Jamnikarjeva 101, 1000 Ljubljana, Slovenia.
[Ti] Título:Revitalisation of metal-contaminated, EDTA-washed soil by addition of unpolluted soil, compost and biochar: Effects on soil enzyme activity, microbial community composition and abundance.
[So] Source:Chemosphere;193:726-736, 2018 Feb.
[Is] ISSN:1879-1298
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Soil remediation mitigates hazards from contaminants but could deprive soils of initial biota and enzymes. Historically contaminated acidic soil from Arnoldstein (Austria) and calcareous soil from Meza (Slovenia) were washed with 30 and 100 mmol kg ethylenediaminetetraacetate (EDTA) to remove 78 and 60% of Pb as a main pollutant. Remediation of the Arnoldstein soil decreased urease activity and increased ß-glucosidase activity, measured in a 15-week experiment. The dehydrogenase activity and microbial gene abundances were not significantly impeded compared to the original soil. Conversely, the use of a high dose of EDTA in the Meza soil, necessary for effective remediation of calcareous soils, resulted in pronouncedly decreased enzyme activities (3.2 times on average) and repressed fungal ITS and increased bacterial 16S rRNA gene abundance. Remediation shifted the microbial community composition in both soils. For revitalisation, the remediated soils were amended with compost, inocula of un-contaminated soil and (Arnoldstein soil) biochar enriched with soil extract. Amendments inconsistently affected the Arnoldstein soil: compost increased the dehydrogenase activity and altered the microbial community composition, biochar enhanced the ß-glucosidase activity, and all amendments decreased the microbial abundance (1.6 times on average). In contrast, amendments efficiently revitalised the remediated Meza soil; compost and soil inoculum returned the enzyme activities back to the baseline in the original soil, increased the fungal abundance above that in the original soil and restored the microbial community composition.
[Mh] Termos MeSH primário: Biota
Carvão Vegetal
Compostagem/métodos
Ácido Edético
Recuperação e Remediação Ambiental/métodos
Microbiologia do Solo
Poluentes do Solo/isolamento & purificação
[Mh] Termos MeSH secundário: Áustria
Ácido Edético/farmacologia
Metais/farmacologia
Eslovênia
Solo/química
Poluentes do Solo/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Metals); 0 (Soil); 0 (Soil Pollutants); 0 (biochar); 16291-96-6 (Charcoal); 9G34HU7RV0 (Edetic Acid)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171128
[St] Status:MEDLINE


  2 / 23559 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29346398
[Au] Autor:Martinez-Andrade JM; Avalos-Borja M; Vilchis-Nestor AR; Sanchez-Vargas LO; Castro-Longoria E
[Ad] Endereço:Departamento de Microbiología, Centro de Investigación Científica y de Educación Superior de Ensenada (CICESE), Ensenada, Baja California, México.
[Ti] Título:Dual function of EDTA with silver nanoparticles for root canal treatment-A novel modification.
[So] Source:PLoS One;13(1):e0190866, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The chelating and antimicrobial capacity of a novel modification of 17% EDTA with silver nanoparticles (AgNPs) (EDTA-AgNPs) was evaluated in-vitro for root canal treatment (RCT). The EDTA-AgNPs solution was characterized by UV-Vis spectroscopy, ζ-potential and high-resolution transmission electron microscopy (HRTEM). Antimicrobial capacity was evaluated against Candida albicans and Staphylococcus aureus in planktonic and biofilm cells by broth macrodilution (24 h) and XTT assays, (1, 10 and 30 min) respectively. The chelating capacity of EDTA-AgNPs was assessed indirectly (smear layer removal) and directly (demineralizing effect) in bovine dentin at two silver concentrations, 16 and 512 µg/ml at 1 and 10 minutes of exposure time. Smear layer removal was evaluated by atomic force microscopy (AFM) and scanning electron microscopy (SEM). The demineralizing effect was determined by atomic absorption spectroscopy (AAS), microhardness test (MH) and X-ray diffractometer (XRD). Synthesized AgNPs were quasi-spherical in shape with an average size of 13.09 ± 8.05 nm. 17% EDTA-AgNPs was effective to inhibit C. albicans and S. aureus in planktonic and biofilm cultures. The smear layer removal and demineralizing effect were similar between 17% EDTA-AgNPs and 17% EDTA treatments. The 17% EDTA-AgNPs solution proved to be an effective antimicrobial agent, and has a similar chelating capacity to 17% EDTA alone. These in-vitro studies strongly suggest that EDTA-AgNPs could be used for effective smear layer removal, having an antimicrobial effect at the same time during RCT.
[Mh] Termos MeSH primário: Ácido Edético/química
Nanopartículas Metálicas/química
Tratamento do Canal Radicular
Prata/química
[Mh] Termos MeSH secundário: Animais
Biofilmes/efeitos dos fármacos
Candida albicans/efeitos dos fármacos
Bovinos
Testes de Sensibilidade Microbiana
Microscopia de Força Atômica
Microscopia Eletrônica de Varredura
Prata/farmacologia
Espectrofotometria Atômica
Staphylococcus aureus/efeitos dos fármacos
Difração de Raios X
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
3M4G523W1G (Silver); 9G34HU7RV0 (Edetic Acid)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180221
[Lr] Data última revisão:
180221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180119
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190866


  3 / 23559 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29025650
[Au] Autor:Lefebvre E; Lembre P; Picard J; El-Guermah L; Seyer D; Larreta Garde V
[Ad] Endereço:ERRMECe Laboratory, University of Cergy-Pontoise, France; Biology Department/ERRMECe, University of Cergy-Pontoise, France.
[Ti] Título:Ephemeral biogels to control anti-biofilm agent delivery: From conception to the construction of an active dressing.
[So] Source:Mater Sci Eng C Mater Biol Appl;82:210-216, 2018 Jan 01.
[Is] ISSN:1873-0191
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Chronic wound colonization by bacterial biofilms is common and can cause various complications. An anti-biofilm strategy was developed around the co-entrapment of a commercially available antiseptic, PHMB (polyhexamethylene biguanide 4mgmL ), with EDTA (Ethylen diamine tetra acetic acid, 20mM) in a gelatin gel. The two active compounds act synergistically against bacterial biofilms, but their efficiency is strongly reduced (16-fold) when entrapped inside the 5% gelatin gel, and they weaken the mechanical properties (50-fold) of the gel. Increasing the gelatin concentration to 7% allows for good mechanical properties but large diffusional constraints. An active ephemeral gel, a chemical gel with controlled hydrolysis, was conceived and developed. When the ephemeral gel was solubilized after 48h, PHMB delivery increased, leading to good anti-biofilm activity. The various gels were examined over 24 and 48h of contact with P. aeruginosa and S. aureus biofilms, two types of bacterial biofilms frequently encountered in chronic wounds. The ephemeral gel eradicated the dense biofilms (>6.10 CFU·cm ) produced by either single or mixed strains; a similar efficiency was measured for biofilms from strains of both laboratory and clinical origin. The formulation was then adapted to develop a dressing prototype that is active against biofilms and fulfils the requirements of an efficient wound care system.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Biguanidas/química
Biofilmes/efeitos dos fármacos
Ácido Edético/química
Géis/química
[Mh] Termos MeSH secundário: Antibacterianos/síntese química
Antibacterianos/química
Bandagens
Biguanidas/farmacologia
Ácido Edético/farmacologia
Gelatina/química
Pseudomonas aeruginosa/fisiologia
Reologia
Staphylococcus aureus/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Biguanides); 0 (Gels); 322U039GMF (polihexanide); 9000-70-8 (Gelatin); 9G34HU7RV0 (Edetic Acid)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180205
[Lr] Data última revisão:
180205
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171014
[St] Status:MEDLINE


  4 / 23559 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Registro de Ensaios Clínicos
Texto completo
[PMID]:28449690
[Au] Autor:Teshome EM; Andang'o PEA; Osoti V; Terwel SR; Otieno W; Demir AY; Prentice AM; Verhoef H
[Ad] Endereço:MRCG Keneba at MRC Unit, Banjul, The Gambia. Emily.teshome@lshtm.ac.uk.
[Ti] Título:Daily home fortification with iron as ferrous fumarate versus NaFeEDTA: a randomised, placebo-controlled, non-inferiority trial in Kenyan children.
[So] Source:BMC Med;15(1):89, 2017 04 28.
[Is] ISSN:1741-7015
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: We aimed to show the non-inferiority of home fortification with a daily dose of 3 mg iron in the form of iron as ferric sodium ethylenediaminetetraacetate (NaFeEDTA) compared with 12.5 mg iron as encapsulated ferrous fumarate in Kenyan children aged 12-36 months. In addition, we updated a recent meta-analysis to assess the efficacy of home fortification with iron-containing powders, with a view to examining diversity in trial results. METHODS: We gave chemoprevention by dihydroartemisinin-piperaquine, albendazole and praziquantel to 338 afebrile children with haemoglobin concentration ≥70 g/L. We randomly allocated them to daily home fortification for 30 days with either placebo, 3 mg iron as NaFeEDTA or 12.5 mg iron as encapsulated ferrous fumarate. We assessed haemoglobin concentration (primary outcome), plasma iron markers, plasma inflammation markers and Plasmodium infection in samples collected at baseline and after 30 days of intervention. We conducted a meta-analysis of randomised controlled trials in pre-school children to assess the effect of home fortification with iron-containing powders on anaemia and haemoglobin concentration at end of intervention. RESULTS: A total of 315 children completed the 30-day intervention period. At baseline, 66.9% of children had inflammation (plasma C-reactive protein concentration >5 mg/L or plasma α -acid glycoprotein concentration >1.0 g/L); in those without inflammation, 42.5% were iron deficient. There was no evidence, either in per protocol analysis or intention-to-treat analysis, that home fortification with either of the iron interventions improved haemoglobin concentration, plasma ferritin concentration, plasma transferrin receptor concentration or erythrocyte zinc protoporphyrin-haem ratio. We also found no evidence of effect modification by iron status, anaemia status and inflammation status at baseline. In the meta-analysis, the effect on haemoglobin concentration was highly heterogeneous between trials (I : 84.1%; p value for test of heterogeneity: <0.0001). CONCLUSIONS: In this population, home fortification with either 3 mg iron as NaFeEDTA or 12.5 mg iron as encapsulated ferrous fumarate was insufficiently efficacious to assess non-inferiority of 3 mg iron as NaFeEDTA compared to 12.5 mg iron as encapsulated ferrous fumarate. Our finding of heterogeneity between trial results should stimulate subgroup analysis or meta-regression to identify population-specific factors that determine efficacy. TRIAL REGISTRATION: The trial was registered with ClinicalTrials.gov ( NCT02073149 ) on 25 February 2014.
[Mh] Termos MeSH primário: Anemia Ferropriva/prevenção & controle
Compostos Férricos/uso terapêutico
Compostos Ferrosos/uso terapêutico
Alimentos Fortificados
[Mh] Termos MeSH secundário: Anemia Ferropriva/sangue
Proteína C-Reativa
Pré-Escolar
Método Duplo-Cego
Ácido Edético/uso terapêutico
Feminino
Ferritinas
Seres Humanos
Lactente
Ferro/sangue
Quênia/epidemiologia
Malária
Masculino
[Pt] Tipo de publicação:JOURNAL ARTICLE; RANDOMIZED CONTROLLED TRIAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Ferric Compounds); 0 (Ferrous Compounds); 9007-41-4 (C-Reactive Protein); 9007-73-2 (Ferritins); 9G34HU7RV0 (Edetic Acid); E1UOL152H7 (Iron); KJ3C78Y22Z (Fe(III)-EDTA); R5L488RY0Q (ferrous fumarate)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:180128
[Lr] Data última revisão:
180128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[Cl] Clinical Trial:ClinicalTrial
[St] Status:MEDLINE
[do] DOI:10.1186/s12916-017-0839-z


  5 / 23559 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29235752
[Au] Autor:Nikolaienko TV; Nikulina VV; Shelest DV; Garmanchuk LV
[Ti] Título:The mechanism of VEGF-mediated endothelial cells survival and proliferation in conditions of unfed-culture.
[So] Source:Ukr Biochem J;88(4):12-9, 2016 Jul-Aug.
[Is] ISSN:2409-4943
[Cp] País de publicação:Ukraine
[La] Idioma:eng
[Ab] Resumo:The mechanisms of VEGF-mediated effects on endothelial cells during cancer development and progression is not clear. In present study the biological effects of VEGF, VEGF-rich culture medium of peritoneal macrophages from mice with Lewis lung carcinoma were studied on MAEC cell line under conditions of unfed culture. We have shown that VEGF increased cell proliferation by the 5th day of culturing vs control and anti-VEGF-treated cells. This effect was associated with increased consumption of glucose and NO production by the 2nd day while decreased ­ on the 5th day of cell culturing. VEGF-mediated NO production was dependent on Ca2+ ions. Block of Ca2+-channels (LaCl3) had more pronounced inhibitory effect vs chelator of Ca2+ ions (EDTA). It was shown that peritoneal macrophages are the main suppliers of VEGF at tumor angiogenesis, as evidenced by the data obtained on model system of endothelial cells synchronized in G0/G1 phase.
[Mh] Termos MeSH primário: Cálcio/metabolismo
Carcinoma Pulmonar de Lewis/metabolismo
Células Endoteliais/efeitos dos fármacos
Macrófagos Peritoneais/secreção
Neovascularização Patológica/metabolismo
Fator A de Crescimento do Endotélio Vascular/farmacologia
[Mh] Termos MeSH secundário: Animais
Anticorpos Monoclonais/farmacologia
Bloqueadores dos Canais de Cálcio/farmacologia
Canais de Cálcio/metabolismo
Carcinoma Pulmonar de Lewis/patologia
Ciclo Celular/efeitos dos fármacos
Linhagem Celular Transformada
Proliferação Celular/efeitos dos fármacos
Sobrevivência Celular/efeitos dos fármacos
Técnicas de Cocultura
Meios de Cultivo Condicionados/química
Meios de Cultivo Condicionados/farmacologia
Ácido Edético/farmacologia
Células Endoteliais/citologia
Células Endoteliais/metabolismo
Feminino
Glucose/metabolismo
Lantânio/farmacologia
Macrófagos Peritoneais/efeitos dos fármacos
Macrófagos Peritoneais/patologia
Camundongos
Neovascularização Patológica/patologia
Óxido Nítrico/biossíntese
Cultura Primária de Células
Fator A de Crescimento do Endotélio Vascular/biossíntese
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Monoclonal); 0 (Calcium Channel Blockers); 0 (Calcium Channels); 0 (Culture Media, Conditioned); 0 (Vascular Endothelial Growth Factor A); 0 (vascular endothelial growth factor A, mouse); 04M8624OXV (lanthanum chloride); 31C4KY9ESH (Nitric Oxide); 6I3K30563S (Lanthanum); 9G34HU7RV0 (Edetic Acid); IY9XDZ35W2 (Glucose); SY7Q814VUP (Calcium)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180116
[Lr] Data última revisão:
180116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171214
[St] Status:MEDLINE
[do] DOI:10.15407/ubj88.04.012


  6 / 23559 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
[PMID]:29185592
[Au] Autor:Leonel LCPC; Miranda CMFC; Coelho TM; Ferreira GAS; Caãada RR; Miglino MA; Lobo SE
[Ad] Endereço:Setor de Anatomia, Departamento de Cirurgia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, SP, Brasil.
[Ti] Título:Decellularization of placentas: establishing a protocol.
[So] Source:Braz J Med Biol Res;51(1):e6382, 2017 Nov 17.
[Is] ISSN:1414-431X
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:Biological biomaterials for tissue engineering purposes can be produced through tissue and/or organ decellularization. The remaining extracellular matrix (ECM) must be acellular and preserve its proteins and physical features. Placentas are organs of great interest because they are discarded after birth and present large amounts of ECM. Protocols for decellularization are tissue-specific and have not been established for canine placentas yet. This study aimed at analyzing a favorable method for decellularization of maternal and fetal portions of canine placentas. Canine placentas were subjected to ten preliminary tests to analyze the efficacy of parameters such as the type of detergents, freezing temperatures and perfusion. Two protocols were chosen for further analyses using histology, scanning electron microscopy, immunofluorescence and DNA quantification. Sodium dodecyl sulfate (SDS) was the most effective detergent for cell removal. Freezing placentas before decellularization required longer periods of incubation in different detergents. Both perfusion and immersion methods were capable of removing cells. Placentas decellularized using Protocol I (1% SDS, 5 mM EDTA, 50 mM TRIS, and 0.5% antibiotic) preserved the ECM structure better, but Protocol I was less efficient to remove cells and DNA content from the ECM than Protocol II (1% SDS, 5 mM EDTA, 0.05% trypsin, and 0.5% antibiotic).
[Mh] Termos MeSH primário: Matriz Extracelular
Feto/citologia
Placenta/citologia
Engenharia Tecidual/métodos
[Mh] Termos MeSH secundário: Animais
Materiais Biocompatíveis
Temperatura Baixa
Colágeno/análise
Cães
Ácido Edético
Feminino
Fibronectinas/análise
Imunofluorescência
Imersão
Laminina/análise
Microscopia Eletrônica de Varredura
Gravidez
Reprodutibilidade dos Testes
Dodecilsulfato de Sódio/farmacologia
Tensoativos/farmacologia
Engenharia Tecidual/veterinária
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biocompatible Materials); 0 (Fibronectins); 0 (Laminin); 0 (Surface-Active Agents); 368GB5141J (Sodium Dodecyl Sulfate); 9007-34-5 (Collagen); 9G34HU7RV0 (Edetic Acid)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171219
[Lr] Data última revisão:
171219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171130
[St] Status:MEDLINE


  7 / 23559 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27777071
[Au] Autor:Kong K; Hiraishi N; Nassar M; Otsuki M; Yiu CKY; Tagami J
[Ad] Endereço:Cariology and Operative Dentistry, Department of Oral Health Sciences, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan; General Dentistry, Roomchang Dental and Aesthetic Hospital, Phnom Penh, Cambodia.
[Ti] Título:Effect of phytic acid etchant on resin-dentin bonding: Monomer penetration and stability of dentin collagen.
[So] Source:J Prosthodont Res;61(3):251-258, 2017 Jul.
[Is] ISSN:2212-4632
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:PURPOSE: Phytic acid (IP6) works well as an etchant in dentin bonding to remove the smear layer due to its acidity and chelating effect. This study compared the etching effect of IP6 with phosphoric acid (PA) and ethylenediaminetetraacetic acid (EDTA) on resin-dentin bond strength, micromorphology of the etched dentin surface and nanoleakage formation along resin-dentin interfaces and compared the protecting effect against collagen degradation. METHODS: Dentin disks and flat dentin surfaces were obtained from extracted human teeth. Specimens were etched with 35% PA (15s), 0.5M EDTA (30s) or 1% IP6 (30s). The surfaces and longitudinal sections of the etched dentin disks were observed using field emission scanning electron microscope (FE-SEM). An etch-and-rinse adhesive was used to create composite build up-specimens for microtensile bond strength (µTBS) testing and nanoleakage observation. To evaluate the effect on collagen degradation, demineralized bovine root dentin blocks were challenged with bacterial collagenase and then observed under light microscope. RESULTS: PA- and EDTA- treated groups showed significantly lower µTBS when compared to IP6-treated group. PA showed distinct nanoleakage and severe collagen degradation. Only slight nanoleakage was detected in IP6 group. IP6 showed better effect than EDTA in preventing collagen degradation induced by bacterial collagenase. CONCLUSIONS: IP6 effectively removed the smear layer and etched dentin, providing high bond strength values and causing minimal nanoleakage and slight collagen degradation.
[Mh] Termos MeSH primário: Ataque Ácido Dentário
Colágeno
Colagem Dentária
Corrosão Dentária
Dentina
Ácido Fítico
Resinas Sintéticas
[Mh] Termos MeSH secundário: Ácido Edético
Seres Humanos
Ácidos Fosfóricos
Proteólise
Resistência à Tração
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Phosphoric Acids); 0 (Resins, Synthetic); 7IGF0S7R8I (Phytic Acid); 9007-34-5 (Collagen); 9G34HU7RV0 (Edetic Acid); E4GA8884NN (phosphoric acid)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:D; IM
[Da] Data de entrada para processamento:161026
[St] Status:MEDLINE


  8 / 23559 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28979628
[Au] Autor:Thuku NW; Shikuku K; Mbugua A
[Ad] Endereço:MSc student at Jomo Kenyatta University of Agriculture and Technology, Head of Haematology and Blood Transfusion Science Section at Mbagathi District Hospital, Nairobi, Kenya.
[Ti] Título:Determination viability of a transfused platelet product by corrected count increment and percentage platelet response.
[So] Source:Pan Afr Med J;27:226, 2017.
[Is] ISSN:1937-8688
[Cp] País de publicação:Uganda
[La] Idioma:eng
[Ab] Resumo:INTRODUCTION: For many years, platelet concentrates have been used for the prevention as well as treatment of bleeding disorders, especially in those patients with haematological problems involving platelet disorders as well as refractoriness, In addition, platelet concentrates (PCs) have been widely used to support patients undergoing bone marrow transplantation or who are receiving myelotoxic treatments. The aim of this study was to determine the quality of platelet concentrates by assessing platelet counts, volume, pH changes, swirling, residue of the red blood cells and white blood cell counts. Assess the in vivo viability of a transfused platelet product using the corrected count increment (CCI) and the percentage platelets response (PPR). This descriptive analysis study was done in Kenyatta National Hospital Blood Transfusion Unit between July 2016 and December 2016. METHODS: The in vitro Platelets concentrates quality was accurately determined and assessed using certain parameters. Platelet concentrates in ethylene diamine tetra acetic acid (EDTA) was used for analysis using Cell-Dyn 3700 analyser. The volume of PCs used was an average of 2mls of PCs, the pH was measured using digitalised Hanna edge pH kit. Agitation was done using Helmer agitator and centrifugation was done using Roto silenta 630 RS centrifuge. The viability of a transfused product was determined using corrected count increment (CCI) and percentage recovery (PR) between 1 and 20-hour after transfusion. Pre and post-transfused whole blood in EDTA collected from the recipients was analysed to access the functional platelets in the circulation. Data analysis was done using SPSS. RESULTS: A total of 384 platelet concentrates were analysed and used in transfusion. The majority 96, (40%) were O Rhesus D+ and the least being AB Rhesus D-at (1%).Centrifugation, separation and agitation was done according to standard procedure (n=384). Only (246 (65%) of the concentrates were found fit for use out of a total of (n=384) leaving 138 (35%) which did not meet the KNH/KNBTS criteria. The minimum specifications for platelet count are 5.5 x (10 ). The duration of 3 days of storage on average, the WBC count (10 ) was Mean ± SD 4.50 ± 3.50. Using the Hanna edge pH kit the pH Mean was ±SD 7.18 ± 8.82 and the used Volume (Mls) was at 55 ± 15. The concentrate was issued within 3 days of processing. After transfusion, the percentage platelet response (PPR) was 72% in male recipients at 1-hour and 30% at 20-hours while 69% in female recipients at 1-hour and 25% at 20-hours. The invivo viability of platelet product had a corrected count increment (CCI) of 75% ≥ 7500 at 1-hour and CCI of18% ≥ 30% at 20-hours in male recipients. In the same study, the female recipients had a CCI of 80% ≥ 7500 at 1-hour and a CCI of 25% ≥ 30% at 20-hours. CONCLUSION: The findings on platelets concentrates quality 65% met platelets transfusion criteria while 35% did not. On preparation of platelets concentrates there was high counts of white blood cells 4.5±3.5×10 than recommended counts by Kenya National Blood Transfusion Services < 0.83×10 . Both percentage platelet response (PPR) and corrected count increment (CCI) were very low at 20 hours compared to British committee for standards haematology criteria for successful increment of platelet products (PPR ≥ 30% and CCI ≥ 7500). Apheresis platelets transfusion can be introduced at KNH and use of leukoreduction performed on the platelet concentrates which are prepared within the Hospital. With such rate of refractoriness, additional tests to confirm the real cause of unviability of platelets in the patients need to be performed. Recipients should be done evaluation of the pattern of refractoriness followed by HLA compatibility testing. In addition, if there is a high, compatible cross-matched, selected apheresis platelet concentrate pint should be transfused. This unviability was due to recipients with either immune-mediated refractoriness or non-immune mediated refractoriness.
[Mh] Termos MeSH primário: Contagem de Leucócitos
Leucócitos
Contagem de Plaquetas
Transfusão de Plaquetas/métodos
[Mh] Termos MeSH secundário: Adolescente
Adulto
Plaquetas/citologia
Criança
Pré-Escolar
Ácido Edético/química
Feminino
Antígenos HLA/imunologia
Teste de Histocompatibilidade
Seres Humanos
Lactente
Quênia
Masculino
Meia-Idade
Plaquetoferese/métodos
Fatores Sexuais
Fatores de Tempo
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (HLA Antigens); 9G34HU7RV0 (Edetic Acid)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171030
[Lr] Data última revisão:
171030
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171006
[St] Status:MEDLINE
[do] DOI:10.11604/pamj.2017.27.226.12116


  9 / 23559 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28875866
[Au] Autor:Herter-Aeberli I; Eliancy K; Rathon Y; Loechl CU; Marhône Pierre J; Zimmermann MB
[Ad] Endereço:1Laboratory of Human Nutrition,ETH Zurich,8092 Zurich,Switzerland.
[Ti] Título:In Haitian women and preschool children, iron absorption from wheat flour-based meals fortified with sodium iron EDTA is higher than that from meals fortified with ferrous fumarate, and is not affected by Helicobacter pylori infection in children.
[So] Source:Br J Nutr;118(4):273-279, 2017 Aug.
[Is] ISSN:1475-2662
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Fe fortification of wheat flour was proposed in Haiti to combat Fe deficiency, but Fe bioavailability from fortificants has never been investigated in Haitian women or preschool children, two key target groups. We aimed to investigate the bioavailability of ferrous fumarate (FeFum), NaFeEDTA and their combination from fortified wheat flour. We recruited twenty-two healthy mother-child pairs in Port au Prince, Haiti, for an Fe-absorption study. We administered stable Fe isotopes as FeFum or NaFeEDTA individually in low-extraction wheat flour bread rolls consumed by all participants in a randomised, cross-over design. In a final, identical meal, consumed only by the women, FeFum+NaFeEDTA was administered. We measured Fe absorption by using erythrocyte incorporation of stable isotopes 14 d after consumption of each meal, and determined Fe status, inflammatory markers and Helicobacter pylori infection. Fe absorption (geometric mean was 9·24 (95 % CI 6·35, 13·44) and 9·26 (95 % CI 7·00, 12·31) from FeFum and 13·06 (95 % CI 9·23, 19·10) and 12·99 (95 % CI 9·18, 18·39) from NaFeEDTA in mothers and children, respectively (P<0·05 between compounds). Fe absorption from FeFum+NaFeEDTA was 11·09 (95 % CI 7·45, 17·34) and did not differ from the other two meals. H. pylori infection did not influence Fe absorption in children. In conclusion, in Haitian women and children, Fe absorption from NaFeEDTA was 40 % higher than from FeFum, and the combination FeFum+NaFeEDTA did not significantly increase Fe absorption compared with FeFum alone. In the context of Haiti, where the high costs of NaFeEDTA may not be affordable, the use of FeFum at 60 mg Fe/kg flour may be a preferable, cost-effective fortification strategy.
[Mh] Termos MeSH primário: Compostos Férricos/farmacocinética
Compostos Ferrosos/farmacocinética
Alimentos Fortificados
Infecções por Helicobacter/complicações
Absorção Intestinal
Ferro/farmacocinética
Triticum/química
[Mh] Termos MeSH secundário: Adulto
Anemia Ferropriva/sangue
Anemia Ferropriva/prevenção & controle
Disponibilidade Biológica
Pão
Pré-Escolar
Dieta
Ácido Edético/sangue
Ácido Edético/farmacocinética
Ácido Edético/uso terapêutico
Eritrócitos/metabolismo
Feminino
Compostos Férricos/sangue
Compostos Férricos/uso terapêutico
Compostos Ferrosos/sangue
Compostos Ferrosos/uso terapêutico
Farinha
Haiti
Infecções por Helicobacter/microbiologia
Helicobacter pylori
Seres Humanos
Ferro/sangue
Ferro/deficiência
Ferro/uso terapêutico
Masculino
Refeições
Adulto Jovem
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RANDOMIZED CONTROLLED TRIAL
[Nm] Nome de substância:
0 (Ferric Compounds); 0 (Ferrous Compounds); 9G34HU7RV0 (Edetic Acid); E1UOL152H7 (Iron); KJ3C78Y22Z (Fe(III)-EDTA); R5L488RY0Q (ferrous fumarate)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170919
[Lr] Data última revisão:
170919
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170907
[St] Status:MEDLINE
[do] DOI:10.1017/S0007114517002045


  10 / 23559 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28814396
[Au] Autor:Paganini D; Uyoga MA; Cercamondi CI; Moretti D; Mwasi E; Schwab C; Bechtler S; Mutuku FM; Galetti V; Lacroix C; Karanja S; Zimmermann MB
[Ad] Endereço:Laboratories of Human Nutrition and.
[Ti] Título:Consumption of galacto-oligosaccharides increases iron absorption from a micronutrient powder containing ferrous fumarate and sodium iron EDTA: a stable-isotope study in Kenyan infants.
[So] Source:Am J Clin Nutr;106(4):1020-1031, 2017 Oct.
[Is] ISSN:1938-3207
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Whether consumption of prebiotics increases iron absorption in infants is unclear. We set out to determine whether prebiotic consumption affects iron absorption from a micronutrient powder (MNP) containing a mixture of ferrous fumarate and sodium iron EDTA (FeFum+NaFeEDTA) in Kenyan infants. Infants ( = 50; aged 6-14 mo) consumed maize porridge that was fortified with an MNP containing FeFum+NaFeEDTA and 7.5 g galacto-oligosaccharides (GOSs) (Fe+GOS group, = 22) or the same MNP without GOSs (Fe group, = 28) each day for 3 wk. Then, on 2 consecutive days, we fed all infants isotopically labeled maize porridge and MNP test meals containing 5 mg Fe as FeFum+Na FeEDTA or ferrous sulfate ( FeSO ). Iron absorption was measured as the erythrocyte incorporation of stable isotopes. Iron markers, fecal pH, and bacterial groups were assessed at baseline and 3 wk. Comparisons within and between groups were done with the use of mixed-effects models. There was a significant group-by-compound interaction on iron absorption ( = 0.011). The median percentages of fractional iron absorption from FeFum+NaFeEDTA and from FeSO in the Fe group were 11.6% (IQR: 6.9-19.9%) and 20.3% (IQR: 14.2-25.7%), respectively, ( < 0.001) and, in the Fe+GOS group, were 18.8% (IQR: 8.3-37.5%) and 25.5% (IQR: 15.1-37.8%), respectively ( = 0.124). Between groups, iron absorption was greater from the FeFum+NaFeEDTA ( = 0.047) in the Fe+GOS group but not from the FeSO ( = 0.653). The relative iron bioavailability from FeFum+NaFeEDTA compared with FeSO was higher in the Fe+GOS group than in the Fe group (88% compared with 63%; = 0.006). There was a significant time-by-group interaction on spp. ( = 0.008) and / / spp. ( = 0.018); / / spp. decreased in the Fe group ( = 0.013), and there was a nonsignificant trend toward higher spp. in the Fe+GOS group ( = 0.099). At 3 wk, iron absorption was negatively correlated with fecal pH ( < 0.001) and positively correlated with / / spp. ( = 0.001). GOS consumption by infants increased iron absorption by 62% from an MNP containing FeFum+NaFeEDTA, thereby possibly reflecting greater colonic iron absorption. This trial was registered at clinicaltrials.gov as NCT02666417.
[Mh] Termos MeSH primário: Compostos Férricos/sangue
Compostos Ferrosos/sangue
Alimentos Fortificados
Absorção Intestinal/efeitos dos fármacos
Ferro/sangue
Oligossacarídeos/farmacologia
Prebióticos
[Mh] Termos MeSH secundário: Anemia Ferropriva/sangue
Anemia Ferropriva/prevenção & controle
Bactérias/crescimento & desenvolvimento
Disponibilidade Biológica
Dieta
Ácido Edético/sangue
Eritrócitos/metabolismo
Feminino
Galactose/farmacologia
Seres Humanos
Lactente
Ferro/farmacocinética
Ferro na Dieta/metabolismo
Ferro na Dieta/farmacocinética
Isótopos
Quênia
Masculino
Micronutrientes
Oligoelementos/sangue
Oligoelementos/farmacocinética
Zea mays
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Ferric Compounds); 0 (Ferrous Compounds); 0 (Iron, Dietary); 0 (Isotopes); 0 (Micronutrients); 0 (Oligosaccharides); 0 (Prebiotics); 0 (Trace Elements); 39R4TAN1VT (ferrous sulfate); 9G34HU7RV0 (Edetic Acid); E1UOL152H7 (Iron); KJ3C78Y22Z (Fe(III)-EDTA); R5L488RY0Q (ferrous fumarate); X2RN3Q8DNE (Galactose)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171013
[Lr] Data última revisão:
171013
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170818
[St] Status:MEDLINE
[do] DOI:10.3945/ajcn.116.145060



página 1 de 2356 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde