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[PMID]: 28745755 [Au] Autor: Isabettini S; Massabni S; Hodzic A; Durovic D; Kohlbrecher J; Ishikawa T; Fischer P; Windhab EJ; Walde P; Kuster S [Ad] Endereço: Laboratory of Food Process Engineering, ETH Zürich, Schmelzbergstrasse 7, 8092 Zürich, Switzerland. stephane.isabettini@hest.ethz.ch. [Ti] Título: Molecular engineering of lanthanide ion chelating phospholipids generating assemblies with a switched magnetic susceptibility. [So] Source: Phys Chem Chem Phys;19(31):20991-21002, 2017 Aug 09. [Is] ISSN: 1463-9084 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: Lanthanide ion (Ln ) chelating amphiphiles are powerful molecules for tailoring the magnetic response of polymolecular assemblies. Mixtures of 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) and 1,2-dimyristoyl-sn-glycero-3-phospho-ethanolamine-diethylene triaminepentaacetate (DMPE-DTPA) complexed to Ln deliver highly magnetically responsive bicelles. Their magnetic properties are readily tuned by changing the bicellar size or the magnetic susceptibility Δχ of the bilayer lipids. The former technique is intrinsically bound to the region of the phase diagram guarantying the formation of bicelles. Methods aiming towards manipulating the Δχ of the bilayer are comparatively more robust, flexible and lacking. Herein, we synthesized a new Ln chelating phospholipid using glutamic acid as a backbone: DMPE-Glu-DTPA. The chelate polyhedron was specifically engineered to alter the Δχ, whilst remaining geometrically similar to DMPE-DTPA. Planar asymmetric assemblies hundreds of nanometers in size were achieved presenting unprecedented magnetic alignments. The DMPE-Glu-DTPA/Ln complex switched the Δχ, achieving perpendicular alignment of assemblies containing Dy and parallel alignment of those containing Tm . Moreover, samples with chelated Yb were more alignable than the Tm chelating counterparts. Such a possibility has never been demonstrated for planar Ln chelating polymolecular assemblies. The physico-chemical properties of these novel assemblies were further studied by monitoring the alignment behavior at different temperatures and by including 16 mol% of cholesterol (Chol-OH) in the phospholipid bilayer. The DMPE-Glu-DTPA/Ln complex and the resulting assemblies are promising candidates for applications in numerous fields including pharmaceutical technologies, structural characterization of membrane biomolecules by NMR spectroscopy, as contrasting agents for magnetic resonance imaging, and for the development of smart optical gels. [Mh] Termos MeSH primário: Elementos da Série dos Lantanídeos/química Fosfolipídeos/química [Mh] Termos MeSH secundário: Colesterol/química Microscopia Crioeletrônica Dimiristoilfosfatidilcolina/química Difusão Dinâmica da Luz Ácido Glutâmico/química Bicamadas Lipídicas/química Espectroscopia de Ressonância Magnética Magnetismo Difração de Nêutrons Ácido Pentético/química Fosfatidiletanolaminas/química Espalhamento a Baixo Ângulo Temperatura Ambiente [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Lanthanoid Series Elements); 0 (Lipid Bilayers); 0 (Phosphatidylethanolamines); 0 (Phospholipids); 3KX376GY7L (Glutamic Acid); 7A314HQM0I (Pentetic Acid); 97C5T2UQ7J (Cholesterol); U86ZGC74V5 (Dimyristoylphosphatidylcholine); Z37OX1ASNK (1,2-dimyristoylphosphatidylethanolamine) [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180212 [Lr] Data última revisão: 180212 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170727 [St] Status: MEDLINE [do] DOI: 10.1039/c7cp03994h

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[PMID]: 28765707 [Au] Autor: Liu Y; Wu X; Sun X; Wang D; Zhong Y; Jiang D; Wang T; Yu D; Zhang N [Ad] Endereço: School of Pharmaceutical Science, Shandong University. [Ti] Título: Design, synthesis, and evaluation of VEGFR-targeted macromolecular MRI contrast agent based on biotin-avidin-specific binding. [So] Source: Int J Nanomedicine;12:5039-5052, 2017. [Is] ISSN: 1178-2013 [Cp] País de publicação: New Zealand [La] Idioma: eng [Ab] Resumo: Developing magnetic resonance imaging (MRI) contrast agents with high relaxivity and specificity was essential to increase MRI diagnostic sensitivity and accuracy. In this study, the MRI contrast agent, vascular endothelial growth factor receptor (VEGFR)-targeted poly (l-lysine) (PLL)-diethylene triamine pentacetate acid (DTPA)-gadolinium (Gd) (VEGFR-targeted PLL-DTPA-Gd, VPDG), was designed and prepared to enhance the MRI diagnosis capacity of tumor. Biotin-PLL-DTPA-Gd was synthesized first, then, VEGFR antibody was linked to biotin-PLL-DTPA-Gd using biotin-avidin reaction. In vitro cytotoxicity study results showed that VPDG had low toxicity to MCF-7 cells and HepG2 cells at experimental concentrations. In cell uptake experiments, VPDG could significantly increase the internalization rates (61.75%±5.22%) in VEGFR-positive HepG2 cells compared to PLL-DTPA-Gd (PDG) (25.16%±4.71%, <0.05). In MRI studies in vitro, significantly higher T1 relaxivity (14.184 mM s ) was observed compared to Magnevist (4.9 mM s ; <0.01). Furthermore, in vivo MRI study results showed that VPDG could significantly enhance the tumor signal intensity and prolong the diagnostic time (from <1 h to 2.5 h). These results indicated that macromolecular VPDG was a promising MRI contrast agent and held great potential for molecular diagnosis of tumor. [Mh] Termos MeSH primário: Meios de Contraste/química Imagem por Ressonância Magnética/métodos Neoplasias Experimentais/diagnóstico por imagem Receptores de Fatores de Crescimento do Endotélio Vascular/metabolismo [Mh] Termos MeSH secundário: Animais Avidina/química Avidina/metabolismo Biotina/química Biotina/metabolismo Meios de Contraste/síntese química Gadolínio/química Gadolínio DTPA Células Hep G2 Seres Humanos Células MCF-7 Camundongos Ácido Pentético/química Poliaminas/química Polilisina/química [Pt] Tipo de publicação: EVALUATION STUDIES; JOURNAL ARTICLE [Nm] Nome de substância: 0 (Contrast Media); 0 (Polyamines); 03K6SX4V2J (diethylenetriamine); 1405-69-2 (Avidin); 25104-18-1 (Polylysine); 6SO6U10H04 (Biotin); 7A314HQM0I (Pentetic Acid); AU0V1LM3JT (Gadolinium); EC 2.7.10.1 (Receptors, Vascular Endothelial Growth Factor); K2I13DR72L (Gadolinium DTPA) [Em] Mês de entrada: 1710 [Cu] Atualização por classe: 171030 [Lr] Data última revisão: 171030 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170803 [St] Status: MEDLINE [do] DOI: 10.2147/IJN.S131878

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[PMID]: 28750057 [Au] Autor: Shen Q; Zhou W; Hu L; Qi Y; Ning H; Chen J; Mo H [Ad] Endereço: Department of Food Science, Henan Institute of Science and Technology, Xinxiang, China. [Ti] Título: Bactericidal activity of alpha-bromocinnamaldehyde against persisters in Escherichia coli. [So] Source: PLoS One;12(7):e0182122, 2017. [Is] ISSN: 1932-6203 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Persisters are tolerant to multiple antibiotics, and widely distributed in bacteria, fungi, parasites, and even cancerous human cell populations, leading to recurrent infections and relapse after therapy. In this study, we investigated the potential of cinnamaldehyde and its derivatives to eradicate persisters in Escherichia coli. The results showed that 200 µg/ml of alpha-bromocinnamaldehyde (Br-CA) was capable of killing all E. coli cells during the exponential phase. Considering the heterogeneous nature of persisters, multiple types of persisters were induced and exposed to Br-CA. Our results indicated that no cells in the ppGpp-overproducing strain or TisB-overexpressing strain survived the treatment of Br-CA although considerable amounts of persisters to ampicillin (Amp) and ciprofloxacin (Cip) were induced. Chemical induction by carbonyl cyanide m-chlorophenylhydrazone (CCCP) led to the formation of more than 10% persister to Amp and Cip in the entire population, and Br-CA still completely eradicated them. In addition, the cells in the stationary phase, which are usually highly recalcitrant to antibiotics treatment, were also completely eradicated by 400 µg/ml of Br-CA. Further studies showed that neither thiourea (hydroxyl-radical scavenger) nor DPTA (Fe3+ chelator to block the hydroxyl-radical) affected the bactericidal efficiency of the Br-CA to kill E. coli, indicating a ROS-independent bactericidal mechanism. Taken together, we concluded that Br-CA compound has a novel bactericidal mechanism and the potential to mitigate antibiotics resistance crisis. [Mh] Termos MeSH primário: Aldeídos/farmacologia Antibacterianos/farmacologia Escherichia coli/efeitos dos fármacos [Mh] Termos MeSH secundário: Toxinas Bacterianas/metabolismo Análise por Conglomerados Testes de Sensibilidade Microbiana Viabilidade Microbiana/efeitos dos fármacos Ácido Pentético/farmacologia Espécies Reativas de Oxigênio/metabolismo Tioureia/farmacologia [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Aldehydes); 0 (Anti-Bacterial Agents); 0 (Bacterial Toxins); 0 (Reactive Oxygen Species); 5443-49-2 (alpha-bromocinnamaldehyde); 7A314HQM0I (Pentetic Acid); GYV9AM2QAG (Thiourea) [Em] Mês de entrada: 1710 [Cu] Atualização por classe: 171004 [Lr] Data última revisão: 171004 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170728 [St] Status: MEDLINE [do] DOI: 10.1371/journal.pone.0182122

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[PMID]: 28683198 [Au] Autor: Spreckelmeyer S; Ramogida CF; Rousseau J; Arane K; Bratanovic I; Colpo N; Jermilova U; Dias GM; Dude I; Jaraquemada-Peláez MG; Bénard F; Schaffer P; Orvig C [Ad] Endereço: Medicinal Inorganic Chemistry Group, Department of Chemistry, University of British Columbia , 2036 Main Mall, Vancouver, British Columbia V6T 1Z1, Canada. [Ti] Título: p-NO -Bn-H neunpa and H neunpa-Trastuzumab: Bifunctional Chelator for Radiometalpharmaceuticals and In Immuno-Single Photon Emission Computed Tomography Imaging. [So] Source: Bioconjug Chem;28(8):2145-2159, 2017 Aug 16. [Is] ISSN: 1520-4812 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Potentially nonadentate (N O ) bifunctional chelator p-SCN-Bn-H neunpa and its immunoconjugate H neunpa-trastuzumab for In radiolabeling are synthesized. The ability of p-SCN-Bn-H neunpa and H neunpa-trastuzumab to quantitatively radiolabel InCl at an ambient temperature within 15 or 30 min, respectively, is presented. Thermodynamic stability determination with In , Bi , and La resulted in high conditional stability constant (pM) values. In vitro human serum stability assays have demonstrated both In complexes to have high stability over 5 days. Mouse biodistribution of [ In][In(p-NO -Bn-neunpa)] , compared to that of [ In][In(p-NH -Bn-CHX-A″-diethylenetriamine pentaacetic acid (DTPA))] , at 1, 4, and 24 h shows fast clearance of both complexes from the mice within 24 h. In a second mouse biodistribution study, the immunoconjugates In-neunpa-trastuzumab and In-CHX-A″-DTPA-trastuzumab demonstrate a similar distribution profile but with slightly lower tumor uptake of In-neunpa-trastuzumab compared to that of In-CHX-A″-DTPA-trastuzumab. These results were also confirmed by immuno-single photon emission computed tomography (immuno-SPECT) imaging in vivo. These initial investigations reveal the acyclic bifunctional chelator p-SCN-Bn-H neunpa to be a promising chelator for In (and other radiometals) with high in vitro stability and also show H neunpa-trastuzumab to be an excellent In chelator with promising biodistribution in mice. [Mh] Termos MeSH primário: Quelantes/química Imunoconjugados/química Radioisótopos de Índio Compostos Organometálicos/química Compostos Radiofarmacêuticos/química Tomografia Computadorizada de Emissão de Fóton Único/métodos Trastuzumab/química [Mh] Termos MeSH secundário: Animais Linhagem Celular Tumoral Quelantes/síntese química Estabilidade de Medicamentos Feminino Seres Humanos Imunoconjugados/farmacocinética Camundongos Compostos Organometálicos/síntese química Ácido Pentético/química Compostos Radiofarmacêuticos/farmacocinética Distribuição Tecidual [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Chelating Agents); 0 (Immunoconjugates); 0 (Indium Radioisotopes); 0 (Organometallic Compounds); 0 (Radiopharmaceuticals); 7A314HQM0I (Pentetic Acid); P188ANX8CK (Trastuzumab) [Em] Mês de entrada: 1709 [Cu] Atualização por classe: 170912 [Lr] Data última revisão: 170912 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170707 [St] Status: MEDLINE [do] DOI: 10.1021/acs.bioconjchem.7b00311

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[PMID]: 28570895 [Au] Autor: Hughes DL; Afsar A; Harwood LM; Jiang T; Laventine DM; Shaw LJ; Hodson ME [Ad] Endereço: Soil Research Centre, Department of Geography and Environmental Science, University of Reading, RG6 6DW, UK. [Ti] Título: Adsorption of Pb and Zn from binary metal solutions and in the presence of dissolved organic carbon by DTPA-functionalised, silica-coated magnetic nanoparticles. [So] Source: Chemosphere;183:519-527, 2017 Sep. [Is] ISSN: 1879-1298 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: The ability of diethylenetriaminepentaacetic acid (DTPA)-functionalised, silica-coated magnetic nanoparticles to adsorb Pb and Zn from single and bi-metallic metal solutions and from solutions containing dissolved organic carbon was assessed. In all experiments 10 mL solutions containing 10 mg of nanoparticles were used. For single metal solutions (10 mg L Pb or Zn) at pH 2 to 8, extraction efficiencies were typically >70%. In bi-metallic experiments, examining the effect of a background of either Zn or Pb (0.025 mmol L ) on the adsorption of variable concentrations (0-0.045 mmol L ) of the other metal (Pb or Zn, respectively) adsorption was well modelled by linear isotherms (R > 0.60; p ≤ 0.001) and Pb was preferentially adsorbed relative to Zn. In dissolved organic carbon experiments, the presence of fulvic acid (0, 2.1 and 21 mg DOC L ) reduced Pb and Zn adsorption from 0.01, 0.1 and 1.0 mmol L solutions. However, even at 21 mg DOC L fulvic acid, extraction efficiencies from 0.01 to 0.1 mmol L solutions remained >80% (Pb) and >50% (Zn). Decreases in extraction efficiency were significant between initial metal concentrations of 0.1 and 1.0 mmol L indicating that at metal loadings between c. 100 mg kg and 300 mg kg occupancy of adsorption sites began to limit further adsorption. The nanoparticles have the potential to perform effectively as metal adsorbents in systems containing more than one metal and dissolved organic carbon at a range of pH values. [Mh] Termos MeSH primário: Benzopiranos/química Poluentes Ambientais/análise Chumbo/análise Nanopartículas de Magnetita/química Ácido Pentético/química Dióxido de Silício/química Zinco/análise [Mh] Termos MeSH secundário: Adsorção Substâncias Húmicas Concentração de Íons de Hidrogênio Modelos Teóricos Soluções Propriedades de Superfície [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Benzopyrans); 0 (Environmental Pollutants); 0 (Humic Substances); 0 (Magnetite Nanoparticles); 0 (Solutions); 2P299V784P (Lead); 7631-86-9 (Silicon Dioxide); 7A314HQM0I (Pentetic Acid); J41CSQ7QDS (Zinc); XII14C5FXV (fulvic acid) [Em] Mês de entrada: 1709 [Cu] Atualização por classe: 170915 [Lr] Data última revisão: 170915 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170602 [St] Status: MEDLINE

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[PMID]: 28542009 [Au] Autor: Poudel D; Bertelli L; Klumpp JA; Waters TL [Ad] Endereço: *Radiation Protection Division, Los Alamos National Laboratory, Los Alamos, NM 87545. [Ti] Título: Interpretation of Urinary Excretion Data From Plutonium Wound Cases Treated With DTPA: Application of Different Models and Approaches. [So] Source: Health Phys;113(1):30-40, 2017 Jul. [Is] ISSN: 1538-5159 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: After a chelation treatment, assessment of intake and doses is the primary concern of an internal dosimetrist. Using the urinary excretion data from two actual wound cases encountered at Los Alamos National Laboratory (LANL), this paper discusses several methods that can be used to interpret intakes from the urinary data collected after one or multiple chelation treatments. One of the methods uses only the data assumed to be unaffected by chelation (data collected beyond 100 d after the last treatment). This method, used by many facilities for official dose records, was implemented by employing maximum likelihood analysis and Bayesian analysis methods. The impacts of an improper assumption about the physicochemical behavior of a radioactive material and the importance of the use of a facility-specific biokinetic model when available have also been demonstrated. Another method analyzed both the affected and unaffected urinary data using an empirical urinary excretion model. This method, although case-specific, was useful in determining the actual intakes and the doses averted or the reduction in body burdens due to chelation treatments. This approach was important in determining the enhancement factors, the behavior of the chelate, and other observations that may be pertinent to several DTPA compartmental modeling approaches being conducted by the health physics community. [Mh] Termos MeSH primário: Modelos Biológicos Ácido Pentético/uso terapêutico Plutônio/urina Lesões por Radiação/prevenção & controle Lesões por Radiação/urina Micção [Mh] Termos MeSH secundário: Terapia por Quelação/métodos Simulação por Computador Seres Humanos Masculino Taxa de Depuração Metabólica Plutônio/farmacocinética Dose de Radiação Monitoramento de Radiação/métodos Resultado do Tratamento Ferimentos Penetrantes/metabolismo Ferimentos Penetrantes/terapia [Pt] Tipo de publicação: CASE REPORTS; JOURNAL ARTICLE [Nm] Nome de substância: 53023GN24M (Plutonium); 7A314HQM0I (Pentetic Acid) [Em] Mês de entrada: 1708 [Cu] Atualização por classe: 170818 [Lr] Data última revisão: 170818 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170526 [St] Status: MEDLINE [do] DOI: 10.1097/HP.0000000000000662

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[PMID]: 28522738 [Au] Autor: Nedrow JR; Josefsson A; Park S; Ranka S; Roy S; Sgouros G [Ad] Endereço: Russell H. Morgan Department of Radiology and Radiological Science, Johns Hopkins University School of Medicine, Baltimore, Maryland. [Ti] Título: Imaging of Programmed Cell Death Ligand 1: Impact of Protein Concentration on Distribution of Anti-PD-L1 SPECT Agents in an Immunocompetent Murine Model of Melanoma. [So] Source: J Nucl Med;58(10):1560-1566, 2017 Oct. [Is] ISSN: 1535-5667 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Programmed cell death ligand 1 (PD-L1) is part of an immune checkpoint system that is essential for preventing autoimmunity and cancer. Recent approaches in immunotherapy that target immune checkpoints have shown great promise in a variety of cancers, including metastatic melanoma. The use of targeted molecular imaging would help identify patients who will best respond to anti-PD-L1 treatment while potentially providing key information to limit immune-related adverse effects. Recently, we developed an antibody-based PD-L1-targeted SPECT agent- In-diethylenetriaminepentaacetic acid (DTPA)-anti-PD-L1-to identify PD-L1-positive tumors in vivo. To best use such PD-L1-targeted imaging agents, it is important, as a first step, to understand how the signal is affected by different parameters. We evaluated the impact of protein concentration on the distribution of In-DTPA-anti-PD-L1 in a murine model of aggressive melanoma. In-DTPA-anti-PD-L1 (dissociation constant, 0.6 ± 0.1 nM) demonstrated increased uptake in B16F10 tumors at protein concentrations equaling or exceeding 1 mg/kg at 24 h and 3 mg/kg at 72 h. At 24 h, the PD-L1-rich spleen and lungs demonstrated decreasing uptake with increasing protein concentration. At 72 h, uptake in the thymus was significantly increased at protein concentrations of 3 mg/kg or greater. Both time points demonstrated increased tracer amounts remaining in circulation as the amount of cold antibody was increased. These studies demonstrate that In-DTPA-anti-PD-L1 is capable of identifying tumors that overexpresses PD-L1 and monitoring the impact of PD-L1-rich organs on the distribution of anti-PD-L1 antibodies. [Mh] Termos MeSH primário: Antígeno B7-H1/imunologia Antígeno B7-H1/metabolismo Imunocompetência Imunoconjugados/imunologia Imunoconjugados/farmacocinética Melanoma/diagnóstico por imagem Tomografia Computadorizada de Emissão de Fóton Único [Mh] Termos MeSH secundário: Animais Linhagem Celular Tumoral Modelos Animais de Doenças Feminino Imunoconjugados/química Melanoma/imunologia Melanoma/metabolismo Camundongos Camundongos Endogâmicos C57BL Ácido Pentético/química Distribuição Tecidual [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (B7-H1 Antigen); 0 (Immunoconjugates); 7A314HQM0I (Pentetic Acid) [Em] Mês de entrada: 1710 [Cu] Atualização por classe: 171116 [Lr] Data última revisão: 171116 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170520 [St] Status: MEDLINE [do] DOI: 10.2967/jnumed.117.193268

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[PMID]: 28255355 [Au] Autor: Buckle T; van der Wal S; van Malderen SJ; Müller L; Kuil J; van Unen V; Peters RJ; van Bemmel ME; McDonnell LA; Velders AH; Koning F; Vanhaeke F; van Leeuwen FW [Ad] Endereço: Interventional Molecular Imaging laboratory, Department of Radiology, Leiden University Medical Center, Leiden, the Netherlands;; Division of Molecular Pathology, Netherlands Cancer Institute- Antoni van Leeuwenhoek hospital (NKI-AvL), Amsterdam, the Netherlands. [Ti] Título: Hybrid Imaging Labels: Providing the Link Between Mass Spectrometry-Based Molecular Pathology and Theranostics. [So] Source: Theranostics;7(3):624-633, 2017. [Is] ISSN: 1838-7640 [Cp] País de publicação: Australia [La] Idioma: eng [Ab] Resumo: BACKGROUND: Development of theranostic concepts that include inductively coupled plasma mass spectrometry (ICP-MS) and laser ablation ICP-MS (LA-ICP-MS) imaging can be hindered by the lack of a direct comparison to more standardly used methods for and evaluation; e.g. fluorescence or nuclear medicine. In this study a bimodal (or rather, hybrid) tracer that contains both a fluorescent dye and a chelate was used to evaluate the existence of a direct link between mass spectrometry (MS) and and molecular imaging findings using fluorescence and radioisotopes. At the same time, the hybrid label was used to determine whether the use of a single isotope label would allow for MS-based diagnostics. METHODS: A hybrid label that contained both a DTPA chelate (that was coordinated with either Ho or In) and a Cy5 fluorescent dye was coupled to the chemokine receptor 4 (CXCR4) targeting peptide Ac-TZ14011 (hybrid-Cy5-Ac-TZ4011). This receptor targeting tracer was used to 1) validate the efficacy of ( Ho-based) mass-cytometry in determining the receptor affinity via comparison with fluorescence-based flow cytometry (Cy5), 2) evaluate the microscopic binding pattern of the tracer in tumor cells using both fluorescence confocal imaging (Cy5) and LA-ICP-MS-imaging ( Ho), 3) compare biodistribution patterns obtained with ICP-MS ( Ho) and radiodetection ( In) after intravenous administration of hybrid-Cy5-Ac-TZ4011 in tumor-bearing mice. Finally, LA-ICP-MS-imaging ( Ho) was linked to fluorescence-based analysis of excised tissue samples (Cy5). RESULTS: Analysis with both mass-cytometry and flow cytometry revealed a similar receptor affinity, respectively 352 ± 141 nM and 245 ± 65 nM (p = 0.08), but with a much lower detection sensitivity for the first modality. LA-ICP-MS imaging ( Ho) enabled clear discrimination between CXCR4 positive and negative cells, but fluorescence microscopy was required to determine the intracellular distribution. biodistribution patterns obtained with ICP-MS ( Ho) and radiodetection ( In) of the hybrid peptide were shown to be similar. Assessment of tracer distribution in excised tissues revealed the location of tracer uptake with both LA-ICP-MS-imaging and fluorescence imaging. CONCLUSION: Lanthanide-isotope chelation expands the scope of fluorescent/radioactive hybrid tracers to include MS-based analytical tools such as mass-cytometry, ICP-MS and LA-ICP-MS imaging in molecular pathology. In contradiction to common expectations, MS detection using a single chelate imaging agent was shown to be feasible, enabling a direct link between nuclear medicine-based imaging and theranostic methods. [Mh] Termos MeSH primário: Espectrometria de Massas/métodos Imagem Multimodal/métodos Patologia Molecular/métodos Receptores CXCR4/análise Nanomedicina Teranóstica/métodos [Mh] Termos MeSH secundário: Animais Carbocianinas/administração & dosagem Citometria de Fluxo Corantes Fluorescentes/administração & dosagem Camundongos Ácido Pentético/administração & dosagem Radioisótopos/administração & dosagem [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (CXCR4 protein, mouse); 0 (Carbocyanines); 0 (Fluorescent Dyes); 0 (Radioisotopes); 0 (Receptors, CXCR4); 0 (cyanine dye 5); 7A314HQM0I (Pentetic Acid) [Em] Mês de entrada: 1710 [Cu] Atualização por classe: 171023 [Lr] Data última revisão: 171023 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170304 [St] Status: MEDLINE [do] DOI: 10.7150/thno.17484

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[PMID]: 28250270 [Au] Autor: Hagimori M; Hatabe E; Sano K; Miyazaki H; Sasaki H; Saji H; Mukai T [Ad] Endereço: Department of Biophysical Chemistry, Kobe Pharmaceutical University. [Ti] Título: An Activatable Fluorescent γ-Polyglutamic Acid Complex for Sentinel Lymph Node Imaging. [So] Source: Biol Pharm Bull;40(3):297-302, 2017. [Is] ISSN: 1347-5215 [Cp] País de publicação: Japan [La] Idioma: eng [Ab] Resumo: Sentinel lymph nodes (SLN) are the first lymph nodes (LN) where cancer cells metastasize from the primary tumor. We designed fluorophore-quencher-based activatable nanoparticles for SLN imaging. We selected TAMRA as a fluorophore and BHQ2 or QSY7 as a quencher. Ternary anionic complexes were constructed with generation 4th polyamidoamine dendrimer (G4) modified with TAMRA and p-SCN-Bn-DTPA (DTPA), polyethyleneimine (PEI) modified with BHQ2 or QSY7, and γ-polyglutamic acid (γ-PGA) by the electrostatic self-assembly system. TAMRA-G4-DTPA/PEI-BHQ2/γ-PGA and TAMRA-G4-DTPA/PEI-QSY7/γ-PGA complexes had a particle size of about 40 nm and a ζ-potential of -50 mV, and showed fluorescence resonance energy transfer (FRET) quenching. Fluorescence microscopy studies demonstrated that TAMRA-G4-DTPA/PEI-QSY7/γ-PGA complex produced intracellular fluorescent signals in the lysosome. During in vivo fluorescent imaging, TAMRA-G4-DTPA/PEI-QSY7/γ-PGA complex enabled the detection of mouse popliteal LN. The fluorophore-quencher conjugated γ-PGA complex based on FRET quenching would be useful for fluorescence-based optical imaging of SLN. [Mh] Termos MeSH primário: Fluorescência Nanopartículas Neoplasias/diagnóstico por imagem Imagem Óptica/métodos Ácido Poliglutâmico/análogos & derivados Linfonodo Sentinela/diagnóstico por imagem [Mh] Termos MeSH secundário: Animais Dendrímeros Corantes Fluorescentes Seres Humanos Metástase Linfática/diagnóstico Metástase Linfática/diagnóstico por imagem Lisossomos Masculino Camundongos Endogâmicos BALB C Neoplasias/patologia Tamanho da Partícula Ácido Pentético Poliaminas Polietilenoimina Rodaminas [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (5-carboxytetramethylrhodamine succinimidyl ester); 0 (Dendrimers); 0 (Fluorescent Dyes); 0 (Poly(amidoamine)); 0 (Polyamines); 0 (Rhodamines); 0 (poly(gamma-glutamic acid)); 25513-46-6 (Polyglutamic Acid); 7A314HQM0I (Pentetic Acid); 9002-98-6 (Polyethyleneimine) [Em] Mês de entrada: 1703 [Cu] Atualização por classe: 170328 [Lr] Data última revisão: 170328 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170303 [St] Status: MEDLINE [do] DOI: 10.1248/bpb.b16-00773

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[PMID]: 28108166 [Au] Autor: Degen DA; Janardan J; Barraclough KA; Schneider HG; Barber T; Barton H; Snell G; Levvey B; Walker RG [Ad] Endereço: Department of Nephrology, Alfred Health, Melbourne, Australia. Electronic address: dovdegen@optusnet.com.au. [Ti] Título: Predictive performance of different kidney function estimation equations in lung transplant patients. [So] Source: Clin Biochem;50(7-8):385-393, 2017 May. [Is] ISSN: 1873-2933 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: BACKGROUND: There has been limited examination of the performance of glomerular filtration rate estimation (eGFR) equations in lung transplant populations. This study aimed to compare the performance of serum creatinine and cystatin C based eGFR equations with Tc-99m diethylenetriaminepentaacetic acid (DTPA) GFR measurements in individuals with end-stage lung disease, either prior to, or following, lung transplantation. METHODS: In this prospective observational study, participants underwent GFR measurements with Tc-99m Pentetate. Measured results were compared with GFR estimates derived from estimation equations [4-variable Modification of Diet in Renal Disease, Cockcroft-Gault, Chronic Kidney Disease Epidemiology Collaboration (CKD-EPI) creatinine, cystatin C and creatinine-cystatin C combined equations]. RESULTS: Ninety-seven individuals were studied (77 post- and 20 wait-listed for transplantation). Median (range) radionucleotide GFR was 56.7ml/min/1.73m (22.8-109.2ml/min/1.73m ). In the study cohort as a whole, the CKD-EPI creatinine-cystatin C combined equation showed the highest performance, but was only slightly superior to the CKD-EPI creatinine equation. However, in individuals with cystic fibrosis, low arm muscle mass and/or low body mass index, all of the creatinine-based equations showed unacceptable performance. In these subgroups, improved GFR estimation was seen with the CKD-EPI cystatin C equation, and predictions were better still using the CKD-EPI creatinine-cystatin C combined equation. CONCLUSIONS: This study shows adequate predictive ability of CKD-EPI creatinine in the cohort as a whole, but unacceptable performance in patients with cystic fibrosis, low arm muscle mass and/or low body mass index. Our findings demonstrate that cystatin C may be a preferable filtration marker in these subgroups. [Mh] Termos MeSH primário: Creatinina/sangue Cistatina C/sangue Taxa de Filtração Glomerular Transplante de Pulmão Renografia por Radioisótopo Compostos Radiofarmacêuticos/administração & dosagem [Mh] Termos MeSH secundário: Adulto Idoso Biomarcadores/sangue Feminino Seres Humanos Masculino Meia-Idade Ácido Pentético/administração & dosagem Valor Preditivo dos Testes [Pt] Tipo de publicação: CLINICAL TRIAL; JOURNAL ARTICLE; OBSERVATIONAL STUDY [Nm] Nome de substância: 0 (Biomarkers); 0 (Cystatin C); 0 (Radiopharmaceuticals); 7A314HQM0I (Pentetic Acid); AYI8EX34EU (Creatinine) [Em] Mês de entrada: 1706 [Cu] Atualização por classe: 170621 [Lr] Data última revisão: 170621 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170122 [St] Status: MEDLINE

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