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  1 / 199 MEDLINE  
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[PMID]:28220566
[Au] Autor:Hirata Y; Yanagi T; Yamaguchi Y; Sato K; Shinkuma S; Hata H; Shimizu H
[Ad] Endereço:Department of Dermatology, Hokkaido University Graduate School of Medicine, 060-8638 Sapporo, Japan.
[Ti] Título:Ulcerative contact dermatitis caused by benzethonium chloride.
[So] Source:Contact Dermatitis;76(3):188-190, 2017 Mar.
[Is] ISSN:1600-0536
[Cp] País de publicação:England
[La] Idioma:eng
[Mh] Termos MeSH primário: Benzetônio/efeitos adversos
Dermatite Alérgica de Contato/etiologia
Desinfetantes/efeitos adversos
Úlcera Cutânea/etiologia
Dedos do Pé
[Mh] Termos MeSH secundário: Adulto
Dermatite Alérgica de Contato/diagnóstico
Feminino
Seres Humanos
Testes do Emplastro
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Disinfectants); 1VU15B70BP (Benzethonium)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170904
[Lr] Data última revisão:
170904
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170222
[St] Status:MEDLINE
[do] DOI:10.1111/cod.12682


  2 / 199 MEDLINE  
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[PMID]:27884428
[Au] Autor:van Boxtel N; Wolfs K; Palacín MG; Van Schepdael A; Adams E
[Ad] Endereço:KU Leuven - University of Leuven, Department of Pharmaceutical and Pharmacological Sciences, Pharmaceutical Analysis, Herestraat 49, O&N2, PB 923, 3000 Leuven, Belgium.
[Ti] Título:Headspace gas chromatography based methodology for the analysis of aromatic substituted quaternary ammonium salts.
[So] Source:J Chromatogr A;1476:105-113, 2016 Dec 09.
[Is] ISSN:1873-3778
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The analysis of quaternary ammonium salts (QAS) using GC is often performed by "in injector" pyrolysis to create volatile degradation products for quantification purposes. Besides the risk of severe system contamination, the application of this approach on aqueous samples is problematic. In this work, the sample is treated in a vial with 2,2-dimethoxypropane (DMP) under acidic catalysis. In addition to the removal of water and sample enrichment, the QAS are decomposed. As HS transfers only volatile compounds to the GC system, contamination is avoided. It was found that depending on the presence of benzyl, phenyl or methyl groups on the quaternary nitrogen; benzyl chloride, N,N-dimethylaniline or chloromethane are formed respectively in the sealed vial. All these can be used as an analytical target. A calibration curve for benzyl chloride could be derived from the pure compound. Chloromethane was generated from pure benzyldimethyldecylammonium chloride (BEDIDE), a pure QAS with benzyl and methyl groups, to construct a secondary calibration curve using a back analysis approach. It has been proven that by quantifying the formed analytical targets, the mass balance for the QAS under investigation was close to 100%. The presented procedure allows the quantification of any aromatic substituted QAS without the need for a matching reference, which is a major advantage over existing CE and LC methods The proposed methodology was validated for mouth sprays containing benzethonium chloride (BZTCl) or benzoxonium chloride (BZOCl) and for denatonium benzoate (DB) in ethylene glycol (EG) based cooling liquids. Results showed that the approach provided excellent linearity (R ≥0.999) and limits of detection around 0.01µg/vial for benzyl chloride. It was found that the reaction product of DMP and glycerol which was also present in the mouthspray and some cooling liquids, caused chromatographic interference with benzyl chloride. Treating those samples in the vial with N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA) after the enrichment step removes the interference and leaves a possible pathway for the simultaneous determination of glycerol in those samples.
[Mh] Termos MeSH primário: Cromatografia Gasosa/métodos
Compostos de Amônio Quaternário/análise
[Mh] Termos MeSH secundário: Acetamidas
Compostos de Benzalcônio/química
Benzetônio/química
Compostos de Benzil/análise
Compostos de Amônio Quaternário/química
Sais/química
Compostos de Trimetilsilil
[Pt] Tipo de publicação:JOURNAL ARTICLE; VALIDATION STUDIES
[Nm] Nome de substância:
0 (Acetamides); 0 (Benzalkonium Compounds); 0 (Benzyl Compounds); 0 (N,O-bis(trimethylsilyl)trifluoroacetamide); 0 (Quaternary Ammonium Compounds); 0 (Salts); 0 (Trimethylsilyl Compounds); 12IMO9R11X (benzoxonium chloride); 1VU15B70BP (Benzethonium); 4YK5Z54AT2 (denatonium benzoate); 83H19HW7K6 (benzyl chloride)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161126
[St] Status:MEDLINE


  3 / 199 MEDLINE  
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[PMID]:27533261
[Au] Autor:Dzhemilev UM; D'yakonov VA; Tuktarova RA; Dzhemileva LU; Ishmukhametova SR; Yunusbaeva MM; de Meijere A
[Ad] Endereço:Laboratory of Catalytic Synthesis, Institute of Petrochemistry and Catalysis of RAS (IPC RAS) , Prospect Octyabrya, 141, 450075, Ufa, Russian Federation.
[Ti] Título:Short Route to the Total Synthesis of Natural Muricadienin and Investigation of Its Cytotoxic Properties.
[So] Source:J Nat Prod;79(8):2039-44, 2016 Aug 26.
[Is] ISSN:1520-6025
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:An original synthesis of the acetogenin muricadienin, the bioprecursor of solamin, has been developed. The key step in the five-step 41% overall yield synthesis is the catalytic cross-cyclomagnesiation reaction of functionally substituted 1,2-dienes with EtMgBr in the presence of Cp2TiCl2 and magnesium metal. It has been demonstrated for the first time that muricadienin exhibits a moderate in vitro inhibitory activity against topoisomerases I and IIα, key cell cycle enzymes. Using flow cytometry, muricadienin was shown to have high cytotoxicity toward the HEK293 kidney cancer cells (IC50 0.39 µM).
[Mh] Termos MeSH primário: Acetogeninas
Antineoplásicos
[Mh] Termos MeSH secundário: Acetogeninas/síntese química
Acetogeninas/química
Acetogeninas/farmacologia
Antineoplásicos/síntese química
Antineoplásicos/química
Antineoplásicos/farmacologia
Benzetônio/química
DNA Topoisomerases Tipo I/metabolismo
DNA Topoisomerases Tipo II/metabolismo
Ensaios de Seleção de Medicamentos Antitumorais
Células HEK293
Seres Humanos
Estrutura Molecular
Inibidores da Topoisomerase I/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acetogenins); 0 (Antineoplastic Agents); 0 (Topoisomerase I Inhibitors); 0 (muricadienin); 1VU15B70BP (Benzethonium); EC 5.99.1.2 (DNA Topoisomerases, Type I); EC 5.99.1.2 (TOP1 protein, human); EC 5.99.1.3 (DNA Topoisomerases, Type II)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170502
[Lr] Data última revisão:
170502
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160818
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jnatprod.6b00335


  4 / 199 MEDLINE  
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[PMID]:27021544
[Au] Autor:Iwamura Y; Hayashi J; Sato T; Sato S; Murakami T; Fujimura T; Sasaki Y; Okada K; Takahashi E; Kikuchi T; Aino M; Noguchi T; Shimazaki Y; Mitani A; Fukuda M
[Ad] Endereço:Department of Periodontology, School of Dentistry, Aichi Gakuin University.
[Ti] Título:Assessment of oral malodor and tonsillar microbiota after gargling with benzethonium chloride.
[So] Source:J Oral Sci;58(1):83-91, 2016.
[Is] ISSN:1880-4926
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:The oropharyngeal area can be a source of halitosis. However, the relationship between healthy tonsillar microbiota and halitosis is poorly understood. We conducted a pilot clinical study to clarify the effect of gargling with an antiseptic agent on tonsillar microbiota in patients with halitosis. Twenty-nine halitosis patients who did not have otolaryngologic disease or periodontitis were assigned randomly to one of three groups: benzethonium chloride (BZC) gargle; placebo gargle; no gargle. Concentrations of volatile sulfur compounds (VSCs) in mouth air, the organoleptic score (ORS) and tongue-coating score (TCS) were measured before and after testing. Tonsillar microbiota were assessed by detection of periodontal pathogens, and profiling with terminal-restriction fragment length polymorphism (T-RFLP) analysis and sequencing of 16SrRNA clone libraries for taxonomic assignment. Gargling with BZC reduced the concentrations of methyl mercaptan and hydrogen sulfide and the ORS, but did not affect the TCS or prevalence of periodontal pathogens. T-RFLP analyses and 16SrRNA clone sequencing showed a tendency for some candidate species to decrease in the test group. Although gargling of the oropharyngeal area with an antiseptic agent can reduce oral malodor, it appears that tonsillar microbiota are not influenced greatly. (J Oral Sci 58, 83-91, 2016).
[Mh] Termos MeSH primário: Anti-Infecciosos Locais/uso terapêutico
Benzetônio/uso terapêutico
Halitose/diagnóstico
Microbiota
Tonsila Palatina/microbiologia
[Mh] Termos MeSH secundário: Método Duplo-Cego
Halitose/microbiologia
Halitose/terapia
Seres Humanos
Projetos Piloto
Polimorfismo de Fragmento de Restrição
Saliva/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RANDOMIZED CONTROLLED TRIAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Infective Agents, Local); 1VU15B70BP (Benzethonium)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170317
[Lr] Data última revisão:
170317
[Sb] Subgrupo de revista:D; IM
[Da] Data de entrada para processamento:160330
[St] Status:MEDLINE
[do] DOI:10.2334/josnusd.58.83


  5 / 199 MEDLINE  
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[PMID]:26219372
[Au] Autor:Mazza R; Mazzette R; McAuliffe O; Jordan K; Fox EM
[Ad] Endereço:University of Sassari, Sassari, Italy.
[Ti] Título:Differential Gene Expression of Three Gene Targets among Persistent and Nonpersistent Listeria monocytogenes Strains in the Presence or Absence of Benzethonium Chloride.
[So] Source:J Food Prot;78(8):1569-73, 2015 Aug.
[Is] ISSN:1944-9097
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Persistence of Listeria monocytogenes strains in food processing environments remains relatively common but is difficult to control. Understanding the basis for such persistence represents an important step in the potential control or eradication of this pathogen from these environments. In this study, reverse transcription PCR was used to determine the relative and absolute expression of selected gene targets (pocR, eutJ, and qacH) among five persistent and four presumed nonpersistent L. monocytogenes strains. The quantification of these genes as markers for the persistent phenotype and the effect of benzethonium chloride (BZT) on their expression was investigated. Although no markers correlated with the ability of strains to persist in food processing facilities were found, expression of pocR was upregulated in three of the five persistent strains, in contrast to the four presumed nonpersistent strains, which showed down-regulation of this gene. These results provide further knowledge of the differential expression of genes of persistent and presumed nonpersistent strains of L. monocytogenes grown in the presence or absence of BZT and identifies upregulation of pocR as a potential response of persistent strains of L. monocytogenes to exposure to BZT.
[Mh] Termos MeSH primário: Proteínas de Bactérias/genética
Benzetônio/farmacologia
Contaminação de Alimentos/análise
Regulação Bacteriana da Expressão Gênica
Listeria monocytogenes/genética
RNA Bacteriano/isolamento & purificação
[Mh] Termos MeSH secundário: Proteínas de Bactérias/metabolismo
Clonagem Molecular
Manipulação de Alimentos
Microbiologia de Alimentos
Marcadores Genéticos
Listeria monocytogenes/efeitos dos fármacos
RNA Bacteriano/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Genetic Markers); 0 (RNA, Bacterial); 1VU15B70BP (Benzethonium)
[Em] Mês de entrada:1603
[Cu] Atualização por classe:150729
[Lr] Data última revisão:
150729
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150730
[St] Status:MEDLINE
[do] DOI:10.4315/0362-028X.JFP-14-510


  6 / 199 MEDLINE  
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[PMID]:26210697
[Au] Autor:Rafsanjany N; Sendker J; Lechtenberg M; Petereit F; Scharf B; Hensel A
[Ad] Endereço:University of Münster, Institute of Pharmaceutical Biology and Phytochemistry, Corrensstrasse 48, D-48149 Münster, Germany.
[Ti] Título:Traditionally used medicinal plants against uncomplicated urinary tract infections: Are unusual, flavan-4-ol- and derhamnosylmaysin derivatives responsible for the antiadhesive activity of extracts obtained from stigmata of Zea mays L. against uropathogenic E. coli and Benzethonium chloride as frequent contaminant faking potential antibacterial activities?
[So] Source:Fitoterapia;105:246-53, 2015 Sep.
[Is] ISSN:1873-6971
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The dried stigmata from Zea mays L. are used traditionally for the treatment of uncomplicated urinary tract infections. A recent screening has indicated that hydroalcoholic extract of the herbal material inhibits the adhesion of uropathogenic Escherichia coli (UPEC) to T24 bladder cells. For verification of these data EtOH-water (1:1) extracts from 4 different batches of Maydis stigmata were investigated. Within an in vitro adhesion assay (UPEC strain 2980 and human T24 bladder cells) a dose-dependent antiadhesive activity against UPEC was verified (IC50 1040µg/mL). Bioassay guided fractionation of M. stigmata, batch S1, by EtOH-water extraction, followed by chromatography on Sephadex LH20 revealed two active fractions (I and XI). Further purification of fraction I and structure elucidation of the isolated compound revealed the presence of significant amounts of the biocide benzethonium chloride as contaminant. Benzethonium chloride was also identified in subsequent investigations in 2 different batches of M. stigmata. The presence of such nondeclared and illegal contaminants in the herbal raw material market has to be discussed intensively. From benzethonium-free raw material (batch S2) as well as from batch S1 fraction XI was further fractionated by MPLC and preparative HPLC, leading to a still complex subfraction XIG, which was analyzed by UHPLC/+ESI-QTOF-MS analysis. Advanced data processing and species-metabolite relationship database revealed the tentatively existence of the unusual C-glycosidic flavones derhamnosylmaysin (6), 3'-deoxyrhamnosylmaysin (4), 3'-O-methylderhamnosylmaysin (3), apiferol (2) and alternanthin (8) which might be related to the antiadhesive activity of this subfraction against UPEC.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Flavonas/farmacologia
Plantas Medicinais/química
Escherichia coli Uropatogênica/efeitos dos fármacos
Zea mays/química
[Mh] Termos MeSH secundário: Aderência Bacteriana/efeitos dos fármacos
Benzetônio/farmacologia
Linhagem Celular
Flores/química
Seres Humanos
Estrutura Molecular
Extratos Vegetais/farmacologia
Bexiga Urinária/citologia
Infecções Urinárias/tratamento farmacológico
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Flavones); 0 (Plant Extracts); 1VU15B70BP (Benzethonium)
[Em] Mês de entrada:1512
[Cu] Atualização por classe:150825
[Lr] Data última revisão:
150825
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150727
[St] Status:MEDLINE


  7 / 199 MEDLINE  
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[PMID]:26204005
[Au] Autor:Livneh A; Youngster I; El-On Y; Berkovitch M; Abu-Kishk I
[Ti] Título:The lack of effectiveness of hyperbaric oxygenation as a treatment for Leishmania major in a mouse model.
[So] Source:Acta Parasitol;60(2):345-9, 2015 Jun.
[Is] ISSN:1896-1851
[Cp] País de publicação:Poland
[La] Idioma:eng
[Ab] Resumo:We aimed to study the effectiveness of hyperbaric oxygen therapy (HOT) (100% oxygen at 2 ATA for 70 minutes each session for 20 consecutive days) on BALB/c male mice infected with Leishmania major. Fifty-one mice were assigned to six groups. Group 1 was treated with HOT from 1 day after the inoculation. In Groups 2-5, treatment began when the cutaneous lesions appeared. Group 2 received HOT only, Group 3 received topical therapy with Leshcutan only, Groups 4 and 5 received a combination of HOT and Leshcutan for 5 and 10 days respectively, and Group 6 did not receive any treatment (control group). When comparing the control group with Group 1, treatment with HOT in Group 1 did not significantly affect the time of the appearance of the lesions. In contrast, mice treated with Leshcutan demonstrated a significant difference in lesion size and spleen dimensions as compared to the rest of the mice (p<0.001). The results show that HOT treatment has no positive effect on the course of Leishmaniasis in a BALB/c mice model infected with Leishmania major. Further studies are needed with a mouse model closer to humans and with different HOT protocols.
[Mh] Termos MeSH primário: Oxigenação Hiperbárica
Leishmania major/efeitos dos fármacos
Leishmaniose Cutânea/tratamento farmacológico
[Mh] Termos MeSH secundário: Animais
Antiprotozoários/uso terapêutico
Benzetônio/análogos & derivados
Benzetônio/uso terapêutico
Modelos Animais de Doenças
Quimioterapia Combinada
Camundongos Endogâmicos BALB C
Paromomicina/uso terapêutico
Resultado do Tratamento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antiprotozoal Agents); 1VU15B70BP (Benzethonium); 61JJC8N5ZK (Paromomycin); WOA57K8S5V (methylbenzethonium chloride)
[Em] Mês de entrada:1604
[Cu] Atualização por classe:161021
[Lr] Data última revisão:
161021
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150724
[St] Status:MEDLINE


  8 / 199 MEDLINE  
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[PMID]:25979325
[Au] Autor:Lee E; Oh C; Kim IS; Kwon IC; Kim S
[Ad] Endereço:Center for Theragnosis, Korea Institute of Science and Technology, 39-1 Hawolgok-dong, Seongbuk-gu, Seoul 136-791, Republic of Korea.
[Ti] Título:Co-delivery of chemosensitizing siRNA and an anticancer agent via multiple monocomplexation-induced hydrophobic association.
[So] Source:J Control Release;210:105-14, 2015 Jul 28.
[Is] ISSN:1873-4995
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Synergistic combination of gene targeting and chemotherapy by co-delivering siRNA and anticancer drugs has widely been investigated to develop siRNA-based therapeutics for cancer treatment. Despite clinical potential of this approach, big challenges still remain such as delivery efficiency or stability/biocompatibility of the siRNA delivery system. Here we report a simple and biocompatible co-delivering formulation based on a unique complexation method, i.e., multiple monocomplexation-induced hydrophobic association between Bcl-2 targeting siRNA and a monocationic anticancer agent (benzethonium chloride, BZT). A colloidal formulation of the hydrophobically associated multiple monocomplex (HMplex) composed of siRNA, BZT and Pluronic F-68 was spontaneously constructed by physical mixing of the ternary constituents. In vitro and in vivo studies revealed that the ternary HMplex with a low charge ratio (N/P=4) possesses a tightly complexed stable nanostructure with Pluronic surface and small colloidal size less than 10nm, which allowed for 1) suitable protection of siRNA in serum-rich physiological environment, 2) efficient intracellular transfection into the cytoplasm, and 3) successful peritumoral co-delivery into the tumor tissue with dense interstitial matrix. Compared to non-targeting HMplexes between scrambled siRNA and BZT, Bcl-2 targeting HMplexes enhanced significantly both mRNA down-regulation by siRNA and apoptosis induction by BZT, and thus greatly suppressed the tumor volume when administered to highly aggressive and resistant human breast cancer xenografts (MDA-MB-231) in mice. These results elucidate that the co-complexed siRNA and BZT were liberated by intracellular decomplexation to trigger a synergistically combined therapeutic action. The successful siRNA/chemodrug co-delivery in vivo via peritumoral route and the greatly promoted therapeutic efficacy thereby represent the clinical potential of HMplexes for adjuvant locoregional cancer treatment by gene-targeted combination therapy.
[Mh] Termos MeSH primário: Antineoplásicos/administração & dosagem
Benzetônio/administração & dosagem
Neoplasias/tratamento farmacológico
Proteínas Proto-Oncogênicas c-bcl-2/genética
RNA Interferente Pequeno/administração & dosagem
[Mh] Termos MeSH secundário: Animais
Antineoplásicos/química
Antineoplásicos/uso terapêutico
Apoptose/efeitos dos fármacos
Benzetônio/química
Benzetônio/uso terapêutico
Linhagem Celular
Linhagem Celular Tumoral
Sobrevivência Celular/efeitos dos fármacos
Terapia Combinada
Feminino
Seres Humanos
Interações Hidrofóbicas e Hidrofílicas
Camundongos Nus
Neoplasias/patologia
Poloxâmero/química
Proteínas Proto-Oncogênicas c-bcl-2/metabolismo
RNA Interferente Pequeno/química
RNA Interferente Pequeno/uso terapêutico
Carga Tumoral/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (Proto-Oncogene Proteins c-bcl-2); 0 (RNA, Small Interfering); 106392-12-5 (Poloxamer); 1VU15B70BP (Benzethonium)
[Em] Mês de entrada:1603
[Cu] Atualização por classe:150616
[Lr] Data última revisão:
150616
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150517
[St] Status:MEDLINE


  9 / 199 MEDLINE  
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[PMID]:25747761
[Au] Autor:Takyi-Williams J; Dong X; Gong H; Wang Y; Jian W; Liu CF; Tang K
[Ad] Endereço:School of Biological Sciences, Nanyang Technological University, Singapore.
[Ti] Título:Application of paper spray-MS in PK studies using sunitinib and benzethonium as model compounds.
[So] Source:Bioanalysis;7(4):413-23, 2015.
[Is] ISSN:1757-6199
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:AIM: Current bioanalytical methods applied in nonclinical PK studies for screening drug candidates demand significant amount of time and resources, hence, the need to develop alternative methods. RESULTS: A proof-of-concept paper spray-MS method for the detection and quantitation of small molecules in plasma has been developed and validated using sunitinib and benzethonium as model compounds. The method includes single oral or intravenous administration of sunitinib to mice and serial micro-volume (20 µl) blood collection at different time intervals. The method is rapid with overall analysis time of 1 min and a full PK profile of sunitinib was obtained from a single mouse. CONCLUSION: The paper spray-MS approach is simple, sensitive and can potentially enable significant reduction of animal use and cost.
[Mh] Termos MeSH primário: Benzetônio/análise
Benzetônio/farmacocinética
Análise Química do Sangue/métodos
Indóis/sangue
Indóis/farmacocinética
Espectrometria de Massas/métodos
Papel
Pirróis/sangue
Pirróis/farmacocinética
[Mh] Termos MeSH secundário: Animais
Estabilidade de Medicamentos
Seres Humanos
Limite de Detecção
Modelos Lineares
Masculino
Camundongos
Camundongos Endogâmicos ICR
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Indoles); 0 (Pyrroles); 1VU15B70BP (Benzethonium); V99T50803M (sunitinib)
[Em] Mês de entrada:1511
[Cu] Atualização por classe:161125
[Lr] Data última revisão:
161125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150310
[St] Status:MEDLINE
[do] DOI:10.4155/bio.14.288


  10 / 199 MEDLINE  
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[PMID]:25446880
[Au] Autor:Gounden V; Sacks DB; Zhao Z
[Ad] Endereço:Department of Laboratory Medicine, NIH Clinical Center, Building 10, Room 2C-249, Bethesda, MD 20892 USA.
[Ti] Título:Interference of cerebrospinal fluid total protein measurement by povidone-iodine contamination.
[So] Source:Clin Chim Acta;440:3-5, 2015 Feb 02.
[Is] ISSN:1873-3492
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: A falsely high cerebrospinal fluid (CSF) total protein (TP) result measured by pyrogallol red (PGR) method was suspected to be caused by preparation of the collection site with povidone-iodine (PVP-iodine) solution. METHODS: CSF TP was evaluated for interference in samples with different final concentrations of PVP-iodine (up to 0.25% PVP and 0.025% iodine) or iodine alone (up to 0.025% iodine) using three methods: PGR, modified biuret and benzethonium chloride (BZTC). Interference exceeding ±20% of the baseline value is considered clinically significant according to the criterion defined by the College of American Pathologists. RESULTS: There were positive interference with the PGR method and negative inference for the BZTC method in CSF samples spiked with PVP-iodine. The PVP-iodine (up to 0.25% PVP and 0.025% iodine) did not cause a clinically significant interference with the modified biuret method. PVP alone without iodine caused a positive interference with the PGR method but did not interfere with the modified biuret or the BZTC method. When the samples were spiked with iodine alone, none of the three methods was affected (change<20%) by iodine concentration up to 0.025%. CONCLUSIONS: Contamination of CSF specimens with PVP-iodine can lead to interference with CSF TP measurements using PGR or BZTC methods.
[Mh] Termos MeSH primário: Proteínas do Líquido Cefalorraquidiano/análise
Química Clínica/métodos
Povidona-Iodo
Manejo de Espécimes/métodos
[Mh] Termos MeSH secundário: Artefatos
Benzetônio
Testes de Química Clínica/métodos
Seres Humanos
Pirogalol/análogos & derivados
Espectrofotometria/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., INTRAMURAL
[Nm] Nome de substância:
0 (Cerebrospinal Fluid Proteins); 01Y4A2QXY0 (Pyrogallol); 1VU15B70BP (Benzethonium); 32638-88-3 (pyrogallol sulfonphthalein); 85H0HZU99M (Povidone-Iodine)
[Em] Mês de entrada:1510
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141203
[St] Status:MEDLINE



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