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[PMID]:28918294
[Au] Autor:Abd Elmoneim H; Sharabi F; Mohy El Din M; Louedec L; Norel X; Senbel A
[Ad] Endereço:Department of Pharmacology and Toxicology, Faculty of Pharmacy, Alexandria University, Egypt.
[Ti] Título:Potassium channels modulate the action but not the synthesis of hydrogen sulfide in rat corpus cavernosum.
[So] Source:Life Sci;189:39-43, 2017 Nov 15.
[Is] ISSN:1879-0631
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:AIM: Hydrogen sulfide (H S) is a newly-introduced gasotransmitter in penile tissues. However, its exact mechanism of action in mediating penile erection is not fully elucidated. The major aim of this study was to examine the role of different K channels in mediating the responses to H S in the corpus cavernosum. MAIN METHODS: Tension studies using isolated rat corpus cavernosum strips were conducted. Endogenous H S production was measured using polarographic technique. Results are expressed as mean±SEM. KEY FINDINGS: l-Cysteine (10 M) stimulated rat corpus cavernosum to produce H S. Blockade of CSE by BCA (10 M) reduced the concentration of H S produced from rat corpus cavernosum significantly. Addition of TEA (10 M) or 4-AP (10 M) didn't have a significant effect on the concentration of H S produced. l-Cysteine (10 -10 M) elicited a concentration-dependent relaxation response which was significantly reduced by blockade of CSE using BCA (10 M). TEA (10 M), 4-AP (10 M) and TEA (10 M) attenuated l-cysteine-induced relaxation significantly. At 10 M, l-cysteine resulted in percentage relaxation of 1.55±0.63, 10.94±1.93 and 1.93±0.80 in presence of TEA (10 M), 4-AP (10 M) and TEA (10 M) respectively compared to 23.78±2.71 as control. Both glibenclamide (10 M) and BaCl (3×10 M) failed to reduce these relaxations significantly. SIGNIFICANCE: H S-induced relaxation of rat corpus cavernosum may be mediated - at least in part - through BK and K channels not by K and K channels. It also seems that K -channels do not contribute to the synthesis of H S.
[Mh] Termos MeSH primário: Sulfeto de Hidrogênio/metabolismo
Pênis/metabolismo
Canais de Potássio/metabolismo
[Mh] Termos MeSH secundário: Alanina/administração & dosagem
Alanina/análogos & derivados
Alanina/farmacologia
Animais
Cisteína/administração & dosagem
Cisteína/farmacologia
Relação Dose-Resposta a Droga
Glibureto/farmacologia
Masculino
Ratos
Ratos Wistar
Tetraetilamônio/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Potassium Channels); 66-40-0 (Tetraethylammonium); 923-01-3 (3-cyanoalanine); K848JZ4886 (Cysteine); OF5P57N2ZX (Alanine); SX6K58TVWC (Glyburide); YY9FVM7NSN (Hydrogen Sulfide)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171023
[Lr] Data última revisão:
171023
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170918
[St] Status:MEDLINE


  2 / 3316 MEDLINE  
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[PMID]:28866099
[Au] Autor:Takano M; Kamei H; Nagahiro M; Kawami M; Yumoto R
[Ad] Endereço:Department of Pharmaceutics and Therapeutics, Graduate School of Biomedical & Health Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8553, Japan. Electronic address: takanom@hiroshima-u.ac.jp.
[Ti] Título:Nicotine transport in lung and non-lung epithelial cells.
[So] Source:Life Sci;188:76-82, 2017 Nov 01.
[Is] ISSN:1879-0631
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:AIMS: Nicotine is rapidly absorbed from the lung alveoli into systemic circulation during cigarette smoking. However, mechanism underlying nicotine transport in alveolar epithelial cells is not well understood to date. In the present study, we characterized nicotine uptake in lung epithelial cell lines A549 and NCI-H441 and in non-lung epithelial cell lines HepG2 and MCF-7. MATERIALS AND METHODS: Characteristics of [ H]nicotine uptake was studied using these cell lines. KEY FINDINGS: Nicotine uptake in A549 cells occurred in a time- and temperature-dependent manner and showed saturation kinetics, with a Km value of 0.31mM. Treatment with some organic cations such as diphenhydramine and pyrilamine inhibited nicotine uptake, whereas treatment with organic cations such as carnitine and tetraethylammonium did not affect nicotine uptake. Extracellular pH markedly affected nicotine uptake, with high nicotine uptake being observed at high pH up to 11.0. Modulation of intracellular pH with ammonium chloride also affected nicotine uptake. Treatment with valinomycin, a potassium ionophore, did not significantly affect nicotine uptake, indicating that nicotine uptake is an electroneutral process. For comparison, we assessed the characteristics of nicotine uptake in another lung epithelial cell line NCI-H441 and in non-lung epithelial cell lines HepG2 and MCF-7. Interestingly, these cell lines showed similar characteristics of nicotine uptake with respect to pH dependency and inhibition by various organic cations. SIGNIFICANCE: The present findings suggest that a similar or the same pH-dependent transport system is involved in nicotine uptake in these cell lines. A novel molecular mechanism of nicotine transport is proposed.
[Mh] Termos MeSH primário: Transporte Biológico/efeitos dos fármacos
Células Epiteliais/metabolismo
Pulmão/metabolismo
Nicotina/farmacocinética
[Mh] Termos MeSH secundário: Carnitina/farmacologia
Células Cultivadas
Difenidramina/farmacologia
Interações Medicamentosas
Seres Humanos
Concentração de Íons de Hidrogênio
Pirilamina/farmacologia
Temperatura Ambiente
Tetraetilamônio/farmacologia
Fatores de Tempo
Trítio/metabolismo
Valinomicina/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
10028-17-8 (Tritium); 2001-95-8 (Valinomycin); 66-40-0 (Tetraethylammonium); 6M3C89ZY6R (Nicotine); 8GTS82S83M (Diphenhydramine); HPE317O9TL (Pyrilamine); S7UI8SM58A (Carnitine)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170929
[Lr] Data última revisão:
170929
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170904
[St] Status:MEDLINE


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[PMID]:28695314
[Au] Autor:Regina TMR; Galluccio M; Scalise M; Pochini L; Indiveri C
[Ad] Endereço:Department DiBEST (Biologia, Ecologia, Scienze della Terra) Unit of Biochemistry and Molecular Biotechnology, University of Calabria, Via P. Bucci 4C, 87036, Arcavacata di Rende, Cosenza, Italy.
[Ti] Título:Bacterial production and reconstitution in proteoliposomes of Solanum lycopersicum CAT2: a transporter of basic amino acids and organic cations.
[So] Source:Plant Mol Biol;94(6):657-667, 2017 Aug.
[Is] ISSN:1573-5028
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:KEY MESSAGE: The vacuolar SlCAT2 was cloned, over-produced in E. coli and reconstituted in proteoliposomes. Arg, Ornithine and Lys were identified as substrates. Unexpectedly, also the organic cations Tetraethylammonium and Acetylcholine were transported indicating involvement of SlCAT2 in signaling. In land plants several transporters are involved in ion and metabolite flux across membranes of cells or intracellular organelles. The vacuolar amino acid transporter CAT2 from Solanum lycopersicum was investigated in this work. SlCAT2 was cloned from tomato flower cDNA, over-produced in Escherichia coli and purified by Nichel-chelating chromatography. For functional studies, the transporter was reconstituted in proteoliposomes. Competence of SlCAT2 for Arg transport was demonstrated measuring uptake of [ H]Arg in proteoliposomes which was trans-stimulated by internal Arg or ornithine. Uptake of [ H]Ornithine and [ H]Lys was also detected at lower efficiency with respect to [ H]Arg. Transport was activated by the presence of intraliposomal ATP suggesting regulation by the nucleotide. The prototype for organic cations tetraethylammonium (TEA) was also transported by SlCAT2. However, scarce reciprocal inhibition between TEA and Arg was found, while the biguanide metformin was able to strongly inhibit uptake of both substrates. These findings suggest that amino acids and organic cations may interact with the transporter through different functional groups some of which are common for the two types of substrates. Interestingly, reconstituted SlCAT2 showed competence for acetylcholine transport, which was also inhibited by metformin. Kinetics of Arg and Ach transport were performed from which Km values of 0.29 and 0.79 mM were derived, respectively.
[Mh] Termos MeSH primário: Proteínas de Transporte/metabolismo
Lycopersicon esculentum/metabolismo
Proteínas de Plantas/metabolismo
Proteolipídeos/metabolismo
[Mh] Termos MeSH secundário: Acetilcolina/metabolismo
Aminoácidos Básicos/metabolismo
Arginina/metabolismo
Transporte Biológico
Proteínas de Transporte/genética
Cátions/metabolismo
Clonagem Molecular
Escherichia coli/genética
Lycopersicon esculentum/genética
Lisina/metabolismo
Ornitina/metabolismo
Proteínas de Plantas/genética
Proteínas de Plantas/isolamento & purificação
Proteínas Recombinantes/genética
Proteínas Recombinantes/isolamento & purificação
Tetraetilamônio/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amino Acids, Basic); 0 (Carrier Proteins); 0 (Cations); 0 (Plant Proteins); 0 (Proteolipids); 0 (Recombinant Proteins); 0 (proteoliposomes); 66-40-0 (Tetraethylammonium); 94ZLA3W45F (Arginine); E524N2IXA3 (Ornithine); K3Z4F929H6 (Lysine); N9YNS0M02X (Acetylcholine)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170814
[Lr] Data última revisão:
170814
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170712
[St] Status:MEDLINE
[do] DOI:10.1007/s11103-017-0632-6


  4 / 3316 MEDLINE  
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[PMID]:28125813
[Au] Autor:Khin PP; Zaw TS; Sohn UD
[Ad] Endereço:Department of Pharmacology, College of Pharmacy, Chung-Ang University, Seoul, Republic of Korea.
[Ti] Título:Signal Transduction Underlying the Inhibitory Mechanism of Fluoxetine on Electrical Field Stimulation Response in Rat Ileal Smooth Muscle.
[So] Source:Pharmacology;99(5-6):216-225, 2017.
[Is] ISSN:1423-0313
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Fluoxetine (FLX), a well-known antidepressant drug under the class of selective serotonin reuptake inhibitor, exerts its action by inhibiting the reuptake of serotonin selectively. In some studies, it has been demonstrated that FLX relaxes the intestinal smooth muscle. In this study, we aimed at studying the signal transduction pathway underlying the muscle relaxation effect of FLX on electrically stimulated rat ileal muscle contraction. To investigate the possible mechanism involved, various antagonists were used. It was found that inhibition with L-NG-nitroarginine methyl ester, ondansetron, GR113808 and bicuculline enhanced the relaxation effect of FLX. However, the effect of FLX was nullified under the presence of atropine, calcium channel modulator (calcium ionophore A23187), and potassium channel blockers (tetraethylammonium chloride, 4-aminopyridine and glybenclamide). Specific pathway-inhibiting antagonists, Y27632 (Rho-kinase inhibitor) and U73122 (phospholipase-C inhibitor) reversed the antagonistic effect of FLX, while ML-9 (myosin light chain kinase inhibitor) and chelerythrine (protein kinase C inhibitor) augmented the FLX-induced inhibition effect. Taken together, we concluded that FLX exerts the inhibitory effect on electric field stimulation response in rat ileal smooth muscle by the inhibition of muscarinic receptors, decrease of intracellular calcium level by inhibiting phospholipase C and opens the potassium channels.
[Mh] Termos MeSH primário: Fluoxetina/farmacologia
Íleo/efeitos dos fármacos
Contração Muscular/efeitos dos fármacos
Músculo Liso/efeitos dos fármacos
Transdução de Sinais/efeitos dos fármacos
[Mh] Termos MeSH secundário: 4-Aminopiridina/farmacologia
Amidas/farmacologia
Animais
Atropina/farmacologia
Azepinas/farmacologia
Benzofenantridinas/farmacologia
Bicuculina/farmacologia
Calcimicina/farmacologia
Interações Medicamentosas
Estimulação Elétrica
Estrenos/farmacologia
Fluoxetina/antagonistas & inibidores
Glibureto/farmacologia
Íleo/fisiologia
Técnicas In Vitro
Indóis/farmacologia
Masculino
Contração Muscular/fisiologia
Músculo Liso/fisiologia
NG-Nitroarginina Metil Éster/farmacologia
Ondansetron/farmacologia
Piridinas/farmacologia
Pirrolidinonas/farmacologia
Ratos
Sulfonamidas/farmacologia
Tetraetilamônio/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amides); 0 (Azepines); 0 (Benzophenanthridines); 0 (Estrenes); 0 (Indoles); 0 (Pyridines); 0 (Pyrrolidinones); 0 (Sulfonamides); 01K63SUP8D (Fluoxetine); 105637-50-1 (ML 9); 112648-68-7 (1-(6-((3-methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl)-1H-pyrrole-2,5-dione); 138381-45-0 (Y 27632); 37H9VM9WZL (Calcimycin); 4AF302ESOS (Ondansetron); 66-40-0 (Tetraethylammonium); 7C0697DR9I (Atropine); BH3B64OKL9 (4-Aminopyridine); E3B045W6X0 (chelerythrine); SX6K58TVWC (Glyburide); V55S2QJN2X (NG-Nitroarginine Methyl Ester); Y37615DVKC (Bicuculline); ZT350OYT3I (GR 113808)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170127
[St] Status:MEDLINE
[do] DOI:10.1159/000449528


  5 / 3316 MEDLINE  
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[PMID]:27744058
[Au] Autor:Cong B; Chai Y; Wang B; Liu S; Shen J; Wang N; Zhang Q; Huang X
[Ad] Endereço:College of Marine Life Sciences, Ocean University of China, Qingdao, Shandong 266003, P.R. China; The First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, P.R. China.
[Ti] Título:Molecular characterization and functional analysis of four teleostean K channels in macrophages of sea perch (Lateolabrax japonicas).
[So] Source:Fish Shellfish Immunol;60:426-435, 2017 Jan.
[Is] ISSN:1095-9947
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Potassium ion channels are one of the most diversely and widely distributed channels, which are involved in all kinds of physiological functions in both excitable and non-excitable cells. The expression of voltage-gated potassium ion (Kv) channels is highly variable according to the state of macrophages activation. Macrophages have an important function in innate immunity against intruding pathogens. They produce a variety of inflammatory and immunoactive molecules that modulate imflammatory responses. Here we show that blockade of K channels by non-selective Kv channel inhibitor tetraethylammonium chloride (TEA), and 4-aminopyridine (4-AP) inhibited proinflammatory cytokines expression, cell proliferation, and reactive oxygen species (ROS) production in LPS-stimulated macrophages of Sea perch (Lateolabrax japonicas). Then we isolated four Kv channels genes (spKv1.1, spKv1.2, spKv1.5 and spKv3.1) in LPS-activated fish macrophages. These channels genes were up-regulated after LPS stimulation except spKv3.1, which remained unchanged during the test. The results of this study indicate that Kv channels could be required for modulating the immune function of fish macrophages.
[Mh] Termos MeSH primário: Citocinas/genética
Proteínas de Peixes/genética
Ativação de Macrófagos/efeitos dos fármacos
Perciformes/genética
Bloqueadores dos Canais de Potássio/farmacologia
Canais de Potássio de Abertura Dependente da Tensão da Membrana/genética
Espécies Reativas de Oxigênio/metabolismo
[Mh] Termos MeSH secundário: 4-Aminopiridina/farmacologia
Sequência de Aminoácidos
Animais
Clonagem Molecular
Citocinas/imunologia
Citocinas/metabolismo
DNA Complementar/genética
DNA Complementar/metabolismo
Proteínas de Peixes/química
Proteínas de Peixes/metabolismo
Imunidade Inata/efeitos dos fármacos
Imunidade Inata/imunologia
Lipopolissacarídeos/farmacologia
Perciformes/imunologia
Perciformes/metabolismo
Filogenia
Canais de Potássio de Abertura Dependente da Tensão da Membrana/química
Canais de Potássio de Abertura Dependente da Tensão da Membrana/metabolismo
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
Reação em Cadeia da Polimerase em Tempo Real/veterinária
Alinhamento de Sequência/veterinária
Tetraetilamônio/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cytokines); 0 (DNA, Complementary); 0 (Fish Proteins); 0 (Lipopolysaccharides); 0 (Potassium Channel Blockers); 0 (Potassium Channels, Voltage-Gated); 0 (RNA, Messenger); 0 (Reactive Oxygen Species); 66-40-0 (Tetraethylammonium); BH3B64OKL9 (4-Aminopyridine)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170327
[Lr] Data última revisão:
170327
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161017
[St] Status:MEDLINE


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[PMID]:27817032
[Au] Autor:Zamoyski VL; Vikhareva EA; Grigoriev VV; Bachurin SO
[Ad] Endereço:Institute of Physiologically Active Compounds, Russian Academy of Sciences, Severnyi proezd 1, Chernogolovka, Moscow oblast, 142432, Russia. vzam@yandex.ru.
[Ti] Título:Tetraethylammonium and 4-aminopyridine block calcium-dependent chloride current in rat cerebellum Purkinje cells.
[So] Source:Dokl Biochem Biophys;470(1):332-334, 2016 Sep.
[Is] ISSN:1608-3091
[Cp] País de publicação:Russia (Federation)
[La] Idioma:eng
[Ab] Resumo:Using patch-clamp method (whole cell configuration), it was shown that tetraethylammonium (TEA) and 4-aminopyridine (4-AP) block calcium-dependent chloride currents in the membrane of freshly isolated cerebellar Purkinje cells of rats (12-15 days). In the concentration range studied (50 µM-10 mM TEA and 100 µM-1 mM 4-AP), both compounds blocked the chloride current at IC 130 µM for TEA and 110 µM for 4-AP. TEA blockade was reversible after washing. The effect of 4-AP at concentrations greater than 100 µM was irreversible: both outward and inward chloride currents were blocked even after the removal of 4-AP from the incubation medium.
[Mh] Termos MeSH primário: 4-Aminopiridina/farmacologia
Cetilpiridínio/metabolismo
Moduladores de Transporte de Membrana/farmacologia
Células de Purkinje/efeitos dos fármacos
Tetraetilamônio/farmacologia
[Mh] Termos MeSH secundário: Animais
Células Cultivadas
Relação Dose-Resposta a Droga
Masculino
Potenciais da Membrana/efeitos dos fármacos
Técnicas de Patch-Clamp
Células de Purkinje/metabolismo
Ratos Wistar
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Membrane Transport Modulators); 66-40-0 (Tetraethylammonium); BH3B64OKL9 (4-Aminopyridine); CUB7JI0JV3 (Cetylpyridinium)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:171019
[Lr] Data última revisão:
171019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161107
[St] Status:MEDLINE


  7 / 3316 MEDLINE  
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[PMID]:27697884
[Au] Autor:Farajnia S; Meijer JH; Michel S
[Ad] Endereço:Leiden University Medical Center, Leiden, The Netherlands Netherlands Institute for Neuroscience, Amsterdam, The Netherlands s.farajnia@nin.knaw.nl.
[Ti] Título:Photoperiod Modulates Fast Delayed Rectifier Potassium Currents in the Mammalian Circadian Clock.
[So] Source:ASN Neuro;8(5), 2016 Oct.
[Is] ISSN:1759-0914
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:One feature of the mammalian circadian clock, situated in the suprachiasmatic nucleus (SCN), is its ability to measure day length and thereby contribute to the seasonal adaptation of physiology and behavior. The timing signal from the SCN, namely the 24 hr pattern of electrical activity, is adjusted according to the photoperiod being broader in long days and narrower in short days. Vasoactive intestinal peptide and gamma-aminobutyric acid play a crucial role in intercellular communication within the SCN and contribute to the seasonal changes in phase distribution. However, little is known about the underlying ionic mechanisms of synchronization. The present study was aimed to identify cellular mechanisms involved in seasonal encoding by the SCN. Mice were adapted to long-day (light-dark 16:8) and short-day (light-dark 8:16) photoperiods and membrane properties as well as K currents activity of SCN neurons were measured using patch-clamp recordings in acute slices. Remarkably, we found evidence for a photoperiodic effect on the fast delayed rectifier K current, that is, the circadian modulation of this ion channel's activation reversed in long days resulting in 50% higher peak values during the night compared with the unaltered day values. Consistent with fast delayed rectifier enhancement, duration of action potentials during the night was shortened and afterhyperpolarization potentials increased in amplitude and duration. The slow delayed rectifier, transient K currents, and membrane excitability were not affected by photoperiod. We conclude that photoperiod can change intrinsic ion channel properties of the SCN neurons, which may influence cellular communication and contribute to photoperiodic phase adjustment.
[Mh] Termos MeSH primário: Canais de Potássio de Retificação Tardia/metabolismo
Neurônios/fisiologia
Fotoperíodo
Núcleo Supraquiasmático/citologia
Núcleo Supraquiasmático/fisiologia
[Mh] Termos MeSH secundário: Animais
Biofísica
Relação Dose-Resposta a Droga
Estimulação Elétrica
Técnicas In Vitro
Masculino
Potenciais da Membrana/efeitos dos fármacos
Camundongos
Camundongos Endogâmicos C57BL
Neurônios/efeitos dos fármacos
Técnicas de Patch-Clamp
Bloqueadores dos Canais de Potássio/farmacologia
Tetraetilamônio/farmacologia
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Delayed Rectifier Potassium Channels); 0 (Potassium Channel Blockers); 66-40-0 (Tetraethylammonium)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171013
[Lr] Data última revisão:
171013
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161005
[St] Status:MEDLINE


  8 / 3316 MEDLINE  
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[PMID]:27625130
[Au] Autor:Liu YH; Wang YP; Wang Y; Ma KT; Si JQ; Li L
[Ad] Endereço:Department of Physiology, the Key Laboratory of Xinjiang Endemic and Ethnic Diseases, Medical College of Shihezi University; Shihezi 832002, China.
[Ti] Título:[Study on the electrophsiological properties in the stria vascularis pericytes in cochlear of guinea pig].
[So] Source:Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi;51(8):600-5, 2016 Aug 07.
[Is] ISSN:1673-0860
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:OBJECTIVE: The purpose of this paper was to study the electrophysiological properties and the type of potassium channels on cell membrane in the stria vascularis pericytes in cochlear of guinea pig. METHODS: Firstly examined the expression of the stria vascularis pericytes by desmin, a marker of pericytes, in cochlear of guinea pig with immunofluorescent method. Using whole-cell patch clamp recording techniques to observe electrophysiological properties in the cochlear pericytes in stria vascularis of guinea pig. RESULTS: Pericytes were predominately distributed in the capillaries of cochlea.The average membrane capacitance, resistance, and potential of a single pericyte in stria vascularis were(5.9±0.3)pF, (2.2±0.3)GΩ and (-30.9±1.2)mV, respectively by using patch clamp technique. In addition, the average current density of cochlear pericyte was voltage-sensitive (Vh from 0 to + 60 mV, in 20 mV steps). The pericytes exhibited outward current and this property could be blocked by TEA (tetraethylammonium) 1 mmol/L, a large-conductance calcium-activated potassium channel(BKCa)inhibitor and 4-AP (4-aminopyridine) 1 mmol/L, a voltage-dependent K(+) channels(KV) channel blocker. TEA blocked the outward current from (296.2±35.9)pA to (163.7±16.8)pA and 4-AP blocked the outward current from (248.7±39.8)pA to (158.0±38.0)pA. CONCLUSION: These results suggest that pericytes in stria vascularis have BKCa and KV channels.
[Mh] Termos MeSH primário: Cóclea/fisiologia
Pericitos/fisiologia
Canais de Potássio/análise
Estria Vascular/citologia
[Mh] Termos MeSH secundário: 4-Aminopiridina/farmacologia
Animais
Fenômenos Eletrofisiológicos/efeitos dos fármacos
Cobaias
Técnicas de Patch-Clamp
Pericitos/química
Pericitos/efeitos dos fármacos
Bloqueadores dos Canais de Potássio/farmacologia
Canais de Potássio Cálcio-Ativados/análise
Estria Vascular/química
Tetraetilamônio/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Potassium Channel Blockers); 0 (Potassium Channels); 0 (Potassium Channels, Calcium-Activated); 66-40-0 (Tetraethylammonium); BH3B64OKL9 (4-Aminopyridine)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170118
[Lr] Data última revisão:
170118
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160915
[St] Status:MEDLINE
[do] DOI:10.3760/cma.j.issn.1673-0860.2016.08.008


  9 / 3316 MEDLINE  
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[PMID]:27409610
[Au] Autor:Pelagalli A; Nardelli A; Fontanella R; Zannetti A
[Ad] Endereço:Dipartimento di Scienze Biomediche Avanzate, Università degli Studi di Napoli "Federico II", Via Pansini No. 5, 80131 Napoli, Italy. alpelaga@unina.it.
[Ti] Título:Inhibition of AQP1 Hampers Osteosarcoma and Hepatocellular Carcinoma Progression Mediated by Bone Marrow-Derived Mesenchymal Stem Cells.
[So] Source:Int J Mol Sci;17(7), 2016 Jul 11.
[Is] ISSN:1422-0067
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:The complex cross-talk between tumor cells and their surrounding stromal environment plays a key role in the pathogenesis of cancer. Among several cell types that constitute the tumor stroma, bone marrow-derived mesenchymal stem cells (BM-MSCs) selectively migrate toward the tumor microenvironment and contribute to the active formation of tumor-associated stroma. Therefore, here we elucidate the involvement of BM-MSCs to promote osteosarcoma (OS) and hepatocellular carcinoma (HCC) cells migration and invasion and deepening the role of specific pathways. We analyzed the function of aquaporin 1 (AQP1), a water channel known to promote metastasis and neoangiogenes. AQP1 protein levels were analyzed in OS (U2OS) and HCC (SNU-398) cells exposed to conditioned medium from BM-MSCs. Tumor cell migration and invasion in response to BM-MSC conditioned medium were evaluated through a wound healing assay and Boyden chamber, respectively. The results showed that the AQP1 level was increased in both tumor cell lines after treatment with BM-MSC conditioned medium. Moreover, BM-MSCs-mediated tumor cell migration and invasion were hampered after treatment with AQP1 inhibitor. These data suggest that the recruitment of human BM-MSCs into the tumor microenvironment might cause OS and HCC cell migration and invasion through involvement of AQP1.
[Mh] Termos MeSH primário: Aquaporina 1/metabolismo
Células da Medula Óssea/citologia
Meios de Cultivo Condicionados/farmacologia
Células-Tronco Hematopoéticas/metabolismo
[Mh] Termos MeSH secundário: Aquaporina 1/antagonistas & inibidores
Neoplasias Ósseas/metabolismo
Neoplasias Ósseas/patologia
Carcinoma Hepatocelular/metabolismo
Carcinoma Hepatocelular/patologia
Linhagem Celular Tumoral
Movimento Celular/efeitos dos fármacos
Proliferação Celular/efeitos dos fármacos
Células-Tronco Hematopoéticas/citologia
Seres Humanos
Neoplasias Hepáticas/metabolismo
Neoplasias Hepáticas/patologia
Osteossarcoma/metabolismo
Osteossarcoma/patologia
Tetraetilamônio/farmacologia
Microambiente Tumoral/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Culture Media, Conditioned); 146410-94-8 (Aquaporin 1); 66-40-0 (Tetraethylammonium)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170327
[Lr] Data última revisão:
170327
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160714
[St] Status:MEDLINE


  10 / 3316 MEDLINE  
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[PMID]:27398978
[Au] Autor:Volkov AG; Nyasani EK; Tuckett C; Blockmon AL; Reedus J; Volkova MI
[Ad] Endereço:Department of Chemistry, Oakwood University, Huntsville, AL 35896, USA. Electronic address: agvolkov@yahoo.com.
[Ti] Título:Cyclic voltammetry of apple fruits: Memristors in vivo.
[So] Source:Bioelectrochemistry;112:9-15, 2016 Dec.
[Is] ISSN:1878-562X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A memristor is a resistor with memory that exhibits a pinched hysteretic relationship in cyclic voltammetry. Recently, we have found memristors in the electrical circuitry of plants and seeds. There are no publications in literature about the possible existence of memristors and electrical differentiators in fruits. Here we found that the electrostimulation of Golden Delicious or Arkansas Black apple fruits by bipolar periodic waves induces hysteresis loops with pinched points in cyclic voltammograms at low frequencies between 0.1MHz and 1MHz. At high frequencies of 1kHz, the pinched hysteresis loop transforms to a non-pinched hysteresis loop instead of a single line I=V/R for ideal memristors because the amplitude of electrical current depends on capacitance of a fruit's tissue and electrodes, frequency and direction of scanning. Electrostimulation of electrical circuits in apple fruits by periodic voltage waves also induces electrotonic potential propagation due to cell-to-cell electrical coupling with electrical differentiators. A differentiator is an electrical circuit in which the output of the circuit is approximately directly proportional to the rate of change of the input. The information gained from electrostimulation can be used to elucidate and to observe electrochemical and electrophysiological properties of electrical circuits in fruits.
[Mh] Termos MeSH primário: Frutas/química
Malus/química
[Mh] Termos MeSH secundário: Impedância Elétrica
Eletroquímica
Frutas/efeitos dos fármacos
Especificidade da Espécie
Tetraetilamônio/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
66-40-0 (Tetraethylammonium)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170126
[Lr] Data última revisão:
170126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160712
[St] Status:MEDLINE



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