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[PMID]:29394010
[Au] Autor:Kleinen J; Venzmer J
[Ti] Título:Streaming potential measurements to understand the rheological properties of surfactant formulations containing anionic and zwitterionic surfactant.
[So] Source:J Cosmet Sci;67(2):59-70, 2016 Mar-Apr.
[Is] ISSN:1525-7886
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Surfactant formulations are often based on an anionic primary surfactant combined with an amphoteric secondary surfactant. One popular option is the combination of lauryl ether sulfate and cocamidopropyl betaine, because such formulations are not only mild but also easy to thicken. Changes in the molecular structure of the betaine in terms of alkyl chain length distribution and headgroup structure do have dramatic effects on the viscosity of these formulations, as can be explained in terms of properties of rod-like micelles and exchange kinetics by oscillatory rheological measurements. The root cause of the effect of the different betaine derivatives on the micellar structure, however, remains unclear when considering rheology only. Although the streaming potential of colloidal objects is typically determined to forecast the stability of dispersions, we have used the streaming potential to characterize micellar solutions of different betaine surfactant structures. It could be shown that (a) the hydrophilicity of the surfactants can be nicely probed by this method and (b) there is a good correlation of these values with the rheological properties of binary mixtures of the betaines with anionic surfactant. Also, the chemical structure of the headgroups has a significant influence on both the isoelectric point and the magnitude of the streaming potential of the zwitterionic surfactants. These effects have again a dramatic influence on the interaction with anionic surfactants, as becomes obvious when looking at the rheology of such mixtures. Therefore, the findings obtained can be utilized to better understand and design surfactant formulations of a desired viscosity profile.
[Mh] Termos MeSH primário: Betaína/análogos & derivados
Polietilenoglicóis/química
Tensoativos/química
Água/química
[Mh] Termos MeSH secundário: Betaína/química
Técnicas Eletroquímicas
Interações Hidrofóbicas e Hidrofílicas
Micelas
Estrutura Molecular
Reologia
Viscosidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Micelles); 0 (Surface-Active Agents); 059QF0KO0R (Water); 26183-44-8 (dodecyl polyoxyethylene sulfuric acid); 30IQX730WE (Polyethylene Glycols); 3SCV180C9W (Betaine); 5OCF3O11KX (cocamidopropyl betaine)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180203
[St] Status:MEDLINE


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[PMID]:29329322
[Au] Autor:Eyring KR; Pedersen BS; Maclean KN; Stabler SP; Yang IV; Schwartz DA
[Ad] Endereço:Department of Medicine, School of Medicine, University of Colorado, Aurora, CO, United States of America.
[Ti] Título:Methylene-tetrahydrofolate reductase contributes to allergic airway disease.
[So] Source:PLoS One;13(1):e0190916, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:RATIONALE: Environmental exposures strongly influence the development and progression of asthma. We have previously demonstrated that mice exposed to a diet enriched with methyl donors during vulnerable periods of fetal development can enhance the heritable risk of allergic airway disease through epigenetic changes. There is conflicting evidence on the role of folate (one of the primary methyl donors) in modifying allergic airway disease. OBJECTIVES: We hypothesized that blocking folate metabolism through the loss of methylene-tetrahydrofolate reductase (Mthfr) activity would reduce the allergic airway disease phenotype through epigenetic mechanisms. METHODS: Allergic airway disease was induced in C57BL/6 and C57BL/6Mthfr-/- mice through house dust mite (HDM) exposure. Airway inflammation and airway hyperresponsiveness (AHR) were measured between the two groups. Gene expression and methylation profiles were generated for whole lung tissue. Disease and molecular outcomes were evaluated in C57BL/6 and C57BL/6Mthfr-/- mice supplemented with betaine. MEASUREMENTS AND MAIN RESULTS: Loss of Mthfr alters single carbon metabolite levels in the lung and serum including elevated homocysteine and cystathionine and reduced methionine. HDM-treated C57BL/6Mthfr-/- mice demonstrated significantly less airway hyperreactivity (AHR) compared to HDM-treated C57BL/6 mice. Furthermore, HDM-treated C57BL/6Mthfr-/- mice compared to HDM-treated C57BL/6 mice have reduced whole lung lavage (WLL) cellularity, eosinophilia, and Il-4/Il-5 cytokine concentrations. Betaine supplementation reversed parts of the HDM-induced allergic airway disease that are modified by Mthfr loss. 737 genes are differentially expressed and 146 regions are differentially methylated in lung tissue from HDM-treated C57BL/6Mthfr-/- mice and HDM-treated C57BL/6 mice. Additionally, analysis of methylation/expression relationships identified 503 significant correlations. CONCLUSION: Collectively, these findings indicate that the loss of folate as a methyl donor is a modifier of allergic airway disease, and that epigenetic and expression changes correlate with this modification. Further investigation into the mechanisms that drive this observation is warranted.
[Mh] Termos MeSH primário: Metilenotetra-Hidrofolato Redutase (NADPH2)/fisiologia
Hipersensibilidade Respiratória/enzimologia
[Mh] Termos MeSH secundário: Animais
Betaína/administração & dosagem
Metilação de DNA
Expressão Gênica
Metilenotetra-Hidrofolato Redutase (NADPH2)/genética
Camundongos
Camundongos Endogâmicos C57BL
Locos de Características Quantitativas
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
3SCV180C9W (Betaine); EC 1.5.1.20 (Methylenetetrahydrofolate Reductase (NADPH2))
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180215
[Lr] Data última revisão:
180215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180113
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190916


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[PMID]:29182016
[Au] Autor:Wang D; Wu X; Long L; Yuan X; Zhang Q; Xue S; Wen S; Yan C; Wang J; Cong W
[Ad] Endereço:a School of Food Engineering and Biological Technology , Tianjin University of Science & Technology , Tianjin , P.R. China.
[Ti] Título:Improved antifouling properties of photobioreactors by surface grafted sulfobetaine polymers.
[So] Source:Biofouling;33(10):970-979, 2017 11.
[Is] ISSN:1029-2454
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:To improve the antifouling (AF) properties of photobioreactors (PBR) for microalgal cultivation, using trihydroxymethyl aminomethane (tris) as the linking agent, a series of polyethylene (PE) films grafted with sulfobetaine (PE-SBMA) with grafting density ranging from 23.11 to 112 µg cm were prepared through surface-initiated atom transfer radical polymerization (SI-ATRP). It was found that the contact angle of PE-SBMA films decreased with the increase in the grafting density. When the grafting density was 101.33 µg cm , it reached 67.27°. Compared with the PE film, the adsorption of protein on the PE-SBMA film decreased by 79.84% and the total weight of solid and absorbed microalgae decreased by 54.58 and 81.69%, respectively. Moreover, the transmittance of PE-SBMA film recovered to 86.03% of the initial value after cleaning, while that of the PE film recovered to only 47.27%. The results demonstrate that the AF properties of PE films were greatly improved on polySBMA-grafted surfaces.
[Mh] Termos MeSH primário: Betaína/análogos & derivados
Incrustação Biológica/prevenção & controle
Microalgas/efeitos dos fármacos
Fotobiorreatores
[Mh] Termos MeSH secundário: Adsorção
Betaína/farmacologia
Microalgas/fisiologia
Polietileno/química
Polímeros/química
Polímeros/farmacologia
Proteínas
Propriedades de Superfície
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Polymers); 0 (Proteins); 3SCV180C9W (Betaine); 8CVU22OCJW (sulfobetaine); 9002-88-4 (Polyethylene)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180215
[Lr] Data última revisão:
180215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171129
[St] Status:MEDLINE
[do] DOI:10.1080/08927014.2017.1394457


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[PMID]:29313349
[Au] Autor:Lu Y; Li H; Geng Y
[Ad] Endereço:Key Laboratory of Food Nutrition and Safety of SDNU, Provincial Key Laboratory of Animal Resistant Biology, College of Life Science, Shandong Normal University , Jinan 250014, China.
[Ti] Título:Analysis of the Effects of δ-Tocopherol on RAW264.7 and K562 Cells Based on H NMR Metabonomics.
[So] Source:J Agric Food Chem;66(4):1039-1046, 2018 Jan 31.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:δ-Tocopherol (δ-TOH) is a form of vitamin E with higher bioactivity. In this study, we studied the bioactivity of δ-TOH using the IC of δ-TOH on RAW264.7 (80 µM) and K562 (110 µM) cells. We compared the differential metabolites from the cell lines with and without δ-TOH treatment by H NMR metabonomics analysis. It was found that δ-TOH affected the protein biosynthesis, betaine metabolism, and urea cycle in various ways in both cell lines. Metabolic levels of the cell lines were changed after treatment with δ-TOH as differential metabolites were produced. The betaine level in RAW264.7 cells was reduced significantly, while the l-lactic acid level in K562 cells was significantly enhanced. The metabolic changes might contribute to the switch of the respiration pattern from aerobic respiration to anaerobic respiration in K562 cells. These results are helpful in further understanding the subtoxicity of δ-TOH.
[Mh] Termos MeSH primário: Espectroscopia de Ressonância Magnética
Metabolômica/métodos
Tocoferóis/farmacologia
[Mh] Termos MeSH secundário: Animais
Betaína/análise
Betaína/metabolismo
Seres Humanos
Células K562
Ácido Láctico/análise
Macrófagos/efeitos dos fármacos
Macrófagos/metabolismo
Redes e Vias Metabólicas/efeitos dos fármacos
Camundongos
Biossíntese de Proteínas/efeitos dos fármacos
Proteínas
Células RAW 264.7
Ureia/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Proteins); 33X04XA5AT (Lactic Acid); 3SCV180C9W (Betaine); 8W8T17847W (Urea); JU84X1II0N (delta-tocopherol); R0ZB2556P8 (Tocopherols)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180110
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b04667


  5 / 3066 MEDLINE  
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[PMID]:29241319
[Au] Autor:Chen H; Huang J; Shi X; Li Y; Liu Y
[Ad] Endereço:School of Food and Biological Engineering, Shaanxi University of Science and Technology Xi?an,China.
[Ti] Título:Effects of six substances on the growth and freeze-drying of Lactobacillus delbrueckii subsp. bulgaricus.
[So] Source:Acta Sci Pol Technol Aliment;16(4):403-412, 2017 Oct-Dec.
[Is] ISSN:1898-9594
[Cp] País de publicação:Poland
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The efficacy of Lactobacillus delbrueckii subsp. bulgaricus as starter cultures for the dairy industry depends largely on the number of viable and active cells. Freeze-drying is the most convenient and successful method to preserve the bacterial cells. However, not all strains survived during freeze-drying. METHODS: The effects of six substances including NaCl, sorbitol, mannitol, mannose, sodium glutamate, betaine added to the MRS medium on the growth and freeze-drying survival rate and viable counts of Lb. delbrueckii subsp. bulgaricus were studied through a single-factor test and Plackett-Burman design. Subsequently, the optimum freeze-drying conditions of Lb. delbrueckii subsp. bulgaricus were determined. RESULTS: Lb. delbrueckii subsp. bulgaricus survival rates were up to the maximum of 42.7%, 45.4%, 23.6%, while the concentrations of NaCl, sorbitol, sodium glutamate were 0.6%, 0.15%, 0.09%, respectively. In the optimum concentration, the viable counts in broth is 6.1, 6.9, 5.13 (×108 CFU/mL), respectively; the viable counts in freeze-drying power are 3.09, 5.2, 2.7 (×1010 CFU/g), respectively. CONCLUSIONS: Three antifreeze factors including NaCl, sorbitol, sodium glutamate have a positive effect on the growth and freeze-drying of Lb. delbrueckii subsp. bulgaricus. The results are beneficial for developing Lb. delbrueckii subsp. bulgaricus.
[Mh] Termos MeSH primário: Crioprotetores/farmacologia
Aditivos Alimentares/farmacologia
Liofilização
Lactobacillus delbrueckii
[Mh] Termos MeSH secundário: Betaína/farmacologia
Lactobacillus delbrueckii/efeitos dos fármacos
Lactobacillus delbrueckii/crescimento & desenvolvimento
Manitol/farmacologia
Viabilidade Microbiana
Cloreto de Sódio/farmacologia
Glutamato de Sódio/farmacologia
Sorbitol/farmacologia
Iogurte/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cryoprotective Agents); 0 (Food Additives); 3OWL53L36A (Mannitol); 3SCV180C9W (Betaine); 451W47IQ8X (Sodium Chloride); 506T60A25R (Sorbitol); W81N5U6R6U (Sodium Glutamate)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180208
[Lr] Data última revisão:
180208
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171216
[St] Status:MEDLINE
[do] DOI:10.17306/J.AFS.0512


  6 / 3066 MEDLINE  
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[PMID]:28457896
[Au] Autor:Ma C; Wang Y; Zhang P; Shi C
[Ad] Endereço:Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, College of Marine Science and Biological Engineering, Qingdao University of Science and Technology, Qingdao 266042, PR China.
[Ti] Título:Accelerated isothermal nucleic acid amplification in betaine-free reaction.
[So] Source:Anal Biochem;530:1-4, 2017 08 01.
[Is] ISSN:1096-0309
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Betaine was used as a common additive to isothermal nucleic acid amplification reactions because of lowering the melting temperature (Tm) of DNA. Herein, we reported a novel finding that betaine was inhibiting the reaction efficiency of isothermal amplification reactions. In this work, we have verified this finding by classical loop-mediated isothermal amplification that the addition of 0.8 M betaine inhibited the efficiency of reaction dropping to approximately 1%. Additionally, we clarified the mechanism of betaine hindering isothermal amplification reactions with a molecular barrier to lower associate rate constant K1 for intermolecular hybridization. This finding would be very significant for studies on the interaction between small molecule substance and DNA, and the development of point-of-care testing because of simplifying reaction system and increasing reaction efficiency.
[Mh] Termos MeSH primário: Betaína/química
Primers do DNA/química
Técnicas de Amplificação de Ácido Nucleico/métodos
Reação em Cadeia da Polimerase/métodos
[Mh] Termos MeSH secundário: Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA Primers); 3SCV180C9W (Betaine)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:180207
[Lr] Data última revisão:
180207
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170502
[St] Status:MEDLINE


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[PMID]:27778219
[Au] Autor:Morris AA; Kozich V; Santra S; Andria G; Ben-Omran TI; Chakrapani AB; Crushell E; Henderson MJ; Hochuli M; Huemer M; Janssen MC; Maillot F; Mayne PD; McNulty J; Morrison TM; Ogier H; O'Sullivan S; Pavlíková M; de Almeida IT; Terry A; Yap S; Blom HJ; Chapman KA
[Ad] Endereço:Institute of Human Development, University of Manchester, Manchester, UK. Andrew.morris@cmft.nhs.uk.
[Ti] Título:Guidelines for the diagnosis and management of cystathionine beta-synthase deficiency.
[So] Source:J Inherit Metab Dis;40(1):49-74, 2017 Jan.
[Is] ISSN:1573-2665
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Cystathionine beta-synthase (CBS) deficiency is a rare inherited disorder in the methionine catabolic pathway, in which the impaired synthesis of cystathionine leads to accumulation of homocysteine. Patients can present to many different specialists and diagnosis is often delayed. Severely affected patients usually present in childhood with ectopia lentis, learning difficulties and skeletal abnormalities. These patients generally require treatment with a low-methionine diet and/or betaine. In contrast, mildly affected patients are likely to present as adults with thromboembolism and to respond to treatment with pyridoxine. In this article, we present recommendations for the diagnosis and management of CBS deficiency, based on a systematic review of the literature. Unfortunately, the quality of the evidence is poor, as it often is for rare diseases. We strongly recommend measuring the plasma total homocysteine concentrations in any patient whose clinical features suggest the diagnosis. Our recommendations may help to standardise testing for pyridoxine responsiveness. Current evidence suggests that patients are unlikely to develop complications if the plasma total homocysteine concentration is maintained below 120 µmol/L. Nevertheless, we recommend keeping the concentration below 100 µmol/L because levels fluctuate and the complications associated with high levels are so serious.
[Mh] Termos MeSH primário: Cistationina beta-Sintase/deficiência
Homocistinúria/dietoterapia
Homocistinúria/tratamento farmacológico
[Mh] Termos MeSH secundário: Betaína/metabolismo
Homocisteína/metabolismo
Seres Humanos
Metionina/metabolismo
Piridoxina/uso terapêutico
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0LVT1QZ0BA (Homocysteine); 3SCV180C9W (Betaine); AE28F7PNPL (Methionine); EC 4.2.1.22 (Cystathionine beta-Synthase); KV2JZ1BI6Z (Pyridoxine)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171204
[Lr] Data última revisão:
171204
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161026
[St] Status:MEDLINE
[do] DOI:10.1007/s10545-016-9979-0


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[PMID]:28946302
[Au] Autor:Zahrina I; Nasikin M; Krisanti E; Mulia K
[Ad] Endereço:Department of Chemical Engineering, Universitas Indonesia, Depok 16424, Indonesia; Department of Chemical Engineering, University of Riau, Pekanbaru 28293, Indonesia.
[Ti] Título:Deacidification of palm oil using betaine monohydrate-based natural deep eutectic solvents.
[So] Source:Food Chem;240:490-495, 2018 Feb 01.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In the palm oil industry, the deacidification process is performed by steam stripping which causes the loss of most of palm oil's natural antioxidants due to high temperature. The liquid-liquid extraction process which is carried out at low temperature is preferable in order to preserve these compounds. The use of hydrated ethanol can reduce the losses of antioxidants, but the ability of this solvent to extract free fatty acids also decreases. Betaine monohydrate-based natural deep eutectic solvents (NADES) have extensive potential for this process. The selectivity of these NADES was determined to select a preferable solvent. The betaine monohydrate-glycerol NADES in a molar ratio of 1:8 was determined to be the preferred solvent with the highest selectivity. This solvent has an efficiency of palmitic acid extraction of 34.14%, and the amount of antioxidants can be preserved in the refined palm oil up to 99%. The compounds are stable during extraction.
[Mh] Termos MeSH primário: Óleos Vegetais/química
[Mh] Termos MeSH secundário: Betaína
Glicerol
Óleo de Palmeira
Solventes
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Oils); 0 (Solvents); 3SCV180C9W (Betaine); 5QUO05548Z (Palm Oil); PDC6A3C0OX (Glycerol)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170927
[St] Status:MEDLINE


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[PMID]:28873565
[Au] Autor:Servillo L; D'Onofrio N; Giovane A; Casale R; Cautela D; Ferrari G; Castaldo D; Balestrieri ML
[Ad] Endereço:Dipartimento di Biochimica, Biofisica e Patologia Generale, Università degli Studi della Campania "Luigi Vanvitelli", Via L. De Crecchio 7, 80138 Napoli, Italy. Electronic address: luigi.servillo@unicampania.it.
[Ti] Título:The betaine profile of cereal flours unveils new and uncommon betaines.
[So] Source:Food Chem;239:234-241, 2018 Jan 15.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:We report the LC-ESI-MS/MS determination of betaines in commercial flours of cereals and pseudocereals most utilized in human nutrition. Results showed that glycine betaine, trigonelline, proline betaine, N -trimethyllysine were metabolites common to all examined flours, whereas an uncommon betaine, valine betaine, and glutamine betaine were present only in flours of barley, rye, oat, durum wheat, winter wheat, Triticum dicoccum and Triticum monococcum. Valine betaine and glutamine betaine, the latter never reported before in plants and animals, are not evenly distributed in the Poaceae family, but their presence or absence in flours depends on the subfamily to which the plant belongs. Interestingly, we also report for the first time the occurrence of pipecolic acid betaine (homostachydrine) and its precursor 1,2-N-methylpipecolic acid in rye flour. These two metabolites were not detected in any other cereal or pseudocereal flour, suggesting their potential role as markers of rye flour occurrence in cereal-based foods.
[Mh] Termos MeSH primário: Farinha
[Mh] Termos MeSH secundário: Animais
Betaína
Grãos Comestíveis
Seres Humanos
Secale
Espectrometria de Massas em Tandem
Triticum
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
3SCV180C9W (Betaine)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170907
[St] Status:MEDLINE


  10 / 3066 MEDLINE  
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[PMID]:28851027
[Au] Autor:Cukier CD; Tourdes A; El-Mazouni D; Guillet V; Nomme J; Mourey L; Milon A; Merdes A; Gervais V
[Ad] Endereço:Institut de Pharmacologie et Biologie Structurale, IPBS, Université de Toulouse, CNRS, UPS, Toulouse, France.
[Ti] Título:NMR secondary structure and interactions of recombinant human MOZART1 protein, a component of the gamma-tubulin complex.
[So] Source:Protein Sci;26(11):2240-2248, 2017 Nov.
[Is] ISSN:1469-896X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Mitotic-spindle organizing protein associated with a ring of γ-tubulin 1 (MOZART1) is an 8.5 kDa protein linked to regulation of γ-tubulin ring complexes (γTuRCs), which are involved in nucleation of microtubules. Despite its small size, MOZART1 represents a challenging target for detailed characterization in vitro. We described herein a protocol for efficient production of recombinant human MOZART1 in Escherichia coli and assessed the properties of the purified protein using a combination of size exclusion chromatography coupled with multiangle light scattering (SEC-MALS), dynamic light scattering (DLS), and nuclear magnetic resonance (NMR) experiments. MOZART1 forms heterogeneous oligomers in solution. We identified optimal detergent and buffer conditions for recording well resolved NMR experiments allowing nearly full protein assignment and identification of three distinct alpha-helical structured regions. Finally, using NMR, we showed that MOZART1 interacts with the N-terminus (residues 1-250) of GCP3 (γ-tubulin complex protein 3). Our data illustrate the capacity of MOZART1 to form oligomers, promoting multiple contacts with a subset of protein partners in the context of microtubule nucleation.
[Mh] Termos MeSH primário: Sequência Conservada
Proteínas Associadas aos Microtúbulos/química
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Arabidopsis/química
Betaína/análogos & derivados
Betaína/química
Sítios de Ligação
Clonagem Molecular
Escherichia coli/genética
Escherichia coli/metabolismo
Expressão Gênica
Seres Humanos
Cinética
Proteínas Associadas aos Microtúbulos/genética
Proteínas Associadas aos Microtúbulos/metabolismo
Microtúbulos/química
Ressonância Magnética Nuclear Biomolecular
Ligação Proteica
Conformação Proteica em alfa-Hélice
Domínios e Motivos de Interação entre Proteínas
Multimerização Proteica
Proteínas Recombinantes/química
Proteínas Recombinantes/genética
Proteínas Recombinantes/metabolismo
Schizosaccharomyces/química
Alinhamento de Sequência
Homologia de Sequência de Aminoácidos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (MOZART1 protein, human); 0 (Microtubule-Associated Proteins); 0 (Recombinant Proteins); 0 (TUBGCP3 protein, human); 3SCV180C9W (Betaine); 8CVU22OCJW (sulfobetaine)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170830
[St] Status:MEDLINE
[do] DOI:10.1002/pro.3282



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