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  1 / 2197 MEDLINE  
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[PMID]:29288663
[Au] Autor:Li X; Sun D; Chen Y; Wang K; He Q; Wang G
[Ad] Endereço:State Key Laboratory of Cultivation Base for Photoelectric Technology and Functional Materials, Laboratory of Optoelectronic Technology of Shaanxi Province, National Center for International Research of Photoelectric Technology & Nano-functional Materials and Application, Institute of Photonics
[Ti] Título:Studying compaction-decompaction of DNA molecules induced by surfactants.
[So] Source:Biochem Biophys Res Commun;495(4):2559-2565, 2018 01 22.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The mechanism and detailed processes of DNA compaction and decompaction are essential for the life activities, as well as for the researches in the molecular biology, genetics and biomedicine. The compaction of two kinds of DNA molecules caused by Cetyltrimethyl Ammonium Bromide (CTAB) and their decompaction induced with sodium dodecyl sulfate (SDS) or excessive amount of CTAB have been investigated with multiple perspectives such as the UV-VIS spectrophotometry, dynamic light scattering, and zeta potential. The compaction phenomenon of DNA can easily be observed when the CTAB combines with the DNA, not just when the molar ratio Q /Q is approximately equal to 1 as the conventional recognition, but also when Q /Q <1,DNA can be compacted; Molecular state of DNA is only changed in the conformational structure, but not in the chemical structure. Finally, a model is suggested to help catch on the biophysical mechanism of DNA chain conformational change.
[Mh] Termos MeSH primário: Compostos de Cetrimônio/química
DNA/química
DNA/ultraestrutura
Modelos Moleculares
Dodecilsulfato de Sódio/química
Tensoativos/química
[Mh] Termos MeSH secundário: Modelos Químicos
Conformação de Ácido Nucleico
Propriedades de Superfície
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Cetrimonium Compounds); 0 (Surface-Active Agents); 368GB5141J (Sodium Dodecyl Sulfate); 9007-49-2 (DNA); Z7FF1XKL7A (cetrimonium)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171231
[St] Status:MEDLINE


  2 / 2197 MEDLINE  
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[PMID]:28759435
[Au] Autor:Demirçivi P; Saygili GN
[Ad] Endereço:Chemical and Process Engineering Department, Yalova University, Yalova, Turkey E-mail: pelindemircivi@gmail.com; Chemical Engineering Department, Istanbul Technical University, Istanbul, Turkey.
[Ti] Título:Response surface modeling of boron adsorption from aqueous solution by vermiculite using different adsorption agents: Box-Behnken experimental design.
[So] Source:Water Sci Technol;76(3-4):515-530, 2017 Jul.
[Is] ISSN:0273-1223
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In this study, a different method was applied for boron removal by using vermiculite as the adsorbent. Vermiculite, which was used in the experiments, was not modified with adsorption agents before boron adsorption using a separate process. Hexadecyltrimethylammonium bromide (HDTMA) and Gallic acid (GA) were used as adsorption agents for vermiculite by maintaining the solid/liquid ratio at 12.5 g/L. HDTMA/GA concentration, contact time, pH, initial boron concentration, inert electrolyte and temperature effects on boron adsorption were analyzed. A three-factor, three-level Box-Behnken design model combined with response surface method (RSM) was employed to examine and optimize process variables for boron adsorption from aqueous solution by vermiculite using HDTMA and GA. Solution pH (2-12), temperature (25-60 °C) and initial boron concentration (50-8,000 mg/L) were chosen as independent variables and coded x , x and x at three levels (-1, 0 and 1). Analysis of variance was used to test the significance of variables and their interactions with 95% confidence limit (α = 0.05). According to the regression coefficients, a second-order empirical equation was evaluated between the adsorption capacity (q ) and the coded variables tested (x ). Optimum values of the variables were also evaluated for maximum boron adsorption by vermiculite-HDTMA (HDTMA-Verm) and vermiculite-GA (GA-Verm).
[Mh] Termos MeSH primário: Silicatos de Alumínio/química
Boro/química
[Mh] Termos MeSH secundário: Adsorção
Compostos de Cetrimônio
Concentração de Íons de Hidrogênio
Modelos Teóricos
Temperatura Ambiente
Poluentes Químicos da Água/análise
Purificação da Água/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Aluminum Silicates); 0 (Cetrimonium Compounds); 0 (Water Pollutants, Chemical); 1318-00-9 (vermiculite); N9E3X5056Q (Boron); Z7FF1XKL7A (cetrimonium)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171013
[Lr] Data última revisão:
171013
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170801
[St] Status:MEDLINE
[do] DOI:10.2166/wst.2017.200


  3 / 2197 MEDLINE  
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[PMID]:28626135
[Au] Autor:Enomoto Y; Imai Y; Tajima K
[Ad] Endereço:Graduate School of Applied Chemistry, Kanagawa University.
[Ti] Título:Adsorption of the Three-phase Emulsion on Various Solid Surfaces.
[So] Source:J Oleo Sci;66(7):689-697, 2017 Jul 01.
[Is] ISSN:1347-3352
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:The present study investigates the adsorption of the three-phase emulsion on various solid/water interfaces. Vesicles can be used as emulsifiers in the three-phase emulsions and act as an independent phase unlike the surfactant used in conventional emulsions; therefore, it is expected that the three-phase emulsion formed by the adhesion of vesicles to the oil/water interface will adsorb on various solid/water interfaces. The cationic three-phase emulsion was prepared to encourage emulsion adsorption on negatively charged solid substrates in water. The emulsifier polyoxyethylene-(10) hydrogenated castor oil was rendered cationic by mixing with the surfactant cetyltrimethylammonium bromide and then used to prepare the cationic three-phase emulsion of hexadecane-in-water. Three solid substrates (silicon, glass, and copper) were dipped in the cationic emulsion and the emulsion was found to adsorb on the solid substrates while maintaining its structure. The amount of hexadecane adsorbed on the various surfaces was investigated by gas chromatography and found to increase with increasing hexadecane concentration in the emulsion and eventually plateaued just like molecular adsorption. The maximum surface coverage of the emulsion on the substrates was approximately 80%. However, even the equivalent nonionic three-phase emulsion was found to adsorb on the three solid surfaces. This was attributed to a novel mechanism of irreversible adhesion via the van der Waals attractive force.
[Mh] Termos MeSH primário: Emulsões/química
[Mh] Termos MeSH secundário: Adsorção
Alcanos/química
Óleo de Rícino/análogos & derivados
Óleo de Rícino/química
Compostos de Cetrimônio/química
Cromatografia Gasosa
Cobre
Vidro
Silicones
Propriedades de Superfície
Tensoativos/química
Água/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alkanes); 0 (Cetrimonium Compounds); 0 (Emulsions); 0 (Silicones); 0 (Surface-Active Agents); 059QF0KO0R (Water); 61791-12-6 (polyethoxylated castor oil); 789U1901C5 (Copper); 8001-79-4 (Castor Oil); F8Z00SHP6Q (n-hexadecane); Z7FF1XKL7A (cetrimonium)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171010
[Lr] Data última revisão:
171010
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170620
[St] Status:MEDLINE
[do] DOI:10.5650/jos.ess16246


  4 / 2197 MEDLINE  
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[PMID]:28617270
[Au] Autor:Thanh LHV; Liu JC
[Ad] Endereço:Department of Chemical Engineering, National Taiwan University of Science and Technology, 43 Keelung Road, Section 4, Taipei 106, Taiwan E-mail: liu1958@mail.ntust.edu.tw; Department of Chemical Engineering, Can Tho University, 3-2 Street, Can Tho, Vietnam.
[Ti] Título:Flotation separation of strontium via phosphate precipitation.
[So] Source:Water Sci Technol;75(11-12):2520-2526, 2017 Jun.
[Is] ISSN:0273-1223
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Flotation separation of strontium (Sr) from wastewater via phosphate precipitation was investigated. While 37.33% of Sr precipitated at highly alkaline pH in the absence of PO , it completely precipitated as Sr (PO ) at a molar ratio ([PO ]:[Sr ]) of 0.62 at a lower pH value. The presence of Ca hindered Sr precipitation, yet it could be overcome by increasing the PO dose. Sodium dodecyl sulfate (SDS) was a better collector for dispersed air flotation of Sr (PO ) than cetyl trimethyl ammonium bromide, or mixed collector systems of SDS and saponin. The highest separation efficiency of 97.5% was achieved at an SDS dose of 40 mg/L. The main mechanism in the precipitate flotation is adsorption of anionic SDS on the positively charged surface of colloidal Sr (PO ) via electrostatic interaction. SDS enhanced the aggregation of Sr (PO ) precipitates as the size increased from 1.65 to 28.0 µm, which was beneficial to separation as well.
[Mh] Termos MeSH primário: Recuperação e Remediação Ambiental/métodos
Fosfatos/química
Estrôncio/química
Poluentes Químicos da Água/química
[Mh] Termos MeSH secundário: Adsorção
Compostos de Cetrimônio/química
Precipitação Química
Dodecilsulfato de Sódio/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cetrimonium Compounds); 0 (Phosphates); 0 (Water Pollutants, Chemical); 368GB5141J (Sodium Dodecyl Sulfate); YZS2RPE8LE (Strontium); Z7FF1XKL7A (cetrimonium)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170616
[St] Status:MEDLINE
[do] DOI:10.2166/wst.2017.129


  5 / 2197 MEDLINE  
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[PMID]:28545732
[Au] Autor:Razmi H; Khosrowshahi EM; Farrokhzadeh S
[Ad] Endereço:Analytical Chemistry Research Lab, Faculty of Basic Sciences, Azarbaijan Shahid Madani University, Tabriz, Iran. Electronic address: h.razmi@azaruniv.edu.
[Ti] Título:Introduction of coiled solid phase microextraction fiber coated by mesoporous silica/cetyltrimethylammonium bromide for ultra-trace environmental analysis.
[So] Source:J Chromatogr A;1506:1-8, 2017 Jul 14.
[Is] ISSN:1873-3778
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:In this study, a tiny coiled cupper wire as a novel solid phase microextraction (SPME) fiber was coated with mesoporous silica/cetyltrimethylammonium bromide (MCM-41/CTAB) as an adsorbent by electrochemically assisted self-assembly method and used for the preconcentration of polycyclic aromatic hydrocarbons (PAHs) as model analytes prior to chromatographic determination. Deposition of MCM-41/CTAB on the coiled SPME (C-SPME) fiber resulted in easily controlled and reproducible SPME coatings. Non-calcined MCM-41/CTAB on C-SPME plays a key role in the adsorption of PAHs. Under the optimized experimental conditions, low detection limits (36-1220pgL ), and wide linear dynamic ranges (R >0.98) were achieved in the range of 0.25-25,000, 0.12-15,000, 0.56-32,000, 4.1-100,000ngL for phenanthrene, anthracene, fluoranthene and pyrene respectively. The reusability of proposed fiber as well as relative standard deviations for repetitive determination of the target analytes was evaluated. The proposed method was successfully applied for determination of PAHs in several real samples.
[Mh] Termos MeSH primário: Compostos de Cetrimônio/química
Hidrocarbonetos Aromáticos Policíclicos/isolamento & purificação
Dióxido de Silício/química
Microextração em Fase Sólida/métodos
Poluentes Químicos da Água/isolamento & purificação
[Mh] Termos MeSH secundário: Adsorção
Limite de Detecção
Hidrocarbonetos Aromáticos Policíclicos/química
Porosidade
Microextração em Fase Sólida/instrumentação
Poluentes Químicos da Água/química
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cetrimonium Compounds); 0 (Polycyclic Aromatic Hydrocarbons); 0 (Water Pollutants, Chemical); 7631-86-9 (Silicon Dioxide); Z7FF1XKL7A (cetrimonium)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171102
[Lr] Data última revisão:
171102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170527
[St] Status:MEDLINE


  6 / 2197 MEDLINE  
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[PMID]:28333050
[Au] Autor:Wang Y; Ni T; Yuan J; Wang C; Liu G
[Ad] Endereço:Key Laboratory of Medical Molecular Probes, School of Basic Medical Sciences, Xinxiang Medical University, Xinxiang 453003, China E-mail: wang2002yl@126.com; School of Environment, Henan Normal University, Henan Key Laboratory for Environmental Pollution Control, Key Laboratory for Yellow River and
[Ti] Título:Oxidative treatment of diclofenac via ferrate(VI) in aqueous media: effect of surfactant additives.
[So] Source:Water Sci Technol;75(5-6):1342-1350, 2017 03.
[Is] ISSN:0273-1223
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The potential reaction of diclofenac (DCF) with ferrate(VI) and influences of coexisting surfactants have not been investigated in depth, and are the focus of this study. The results demonstrated that DCF reacted effectively and rapidly with Fe(VI) and approximately 75% of DCF (0.03 mM) was removed by excess Fe(VI) (0.45 mM) within 10 min. All of the reactions followed pseudo first-order kinetics with respect to DCF and Fe(VI), where the apparent second-order rate constant (k ) was 5.07 M s at pH 9.0. Furthermore, the degradation efficiencies of DCF were clearly dependent on the concentrations of dissolved organic matter additives in the substrate solution. Primarily, inhibitory effects were observed with the samples that contained anionic (sodium dodecyl-benzene sulfonate, SDBS) or non-ionic (Tween-80) surfactants, which have been attributed to the side reactions between Fe(VI) and surfactants, which led to a reduction in the available oxidant for DCF destruction. Furthermore, the addition of a cationic surfactant (cetyltrimethyl ammonium bromide, CTAB) and humic acid (HA) conveyed significantly promotional effects on the DCF-Fe(VI) reaction. The rate enhancement effect for CTAB might be due to micellar surface catalysis, through the Coulomb attraction between the reactants and positively charged surfactants, while the catalytic action for HA resulted from the additional oxidation of Fe(V)/Fe(IV) in the presence of HA. The results provided the basic knowledge required to understand the environmental relevance of DCF oxidation via Fe(VI) in the presence of surfactant additives.
[Mh] Termos MeSH primário: Diclofenaco/química
Ferro/química
Tensoativos/química
Poluentes Químicos da Água/análise
[Mh] Termos MeSH secundário: Benzenossulfonatos/química
Catálise
Compostos de Cetrimônio/química
Substâncias Húmicas/análise
Cinética
Oxirredução
Polissorbatos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Benzenesulfonates); 0 (Cetrimonium Compounds); 0 (Humic Substances); 0 (Polysorbates); 0 (Surface-Active Agents); 0 (Water Pollutants, Chemical); 144O8QL0L1 (Diclofenac); 16836-06-9 (ferrate ion); 60NSK897G9 (dodecylbenzenesulfonic acid); E1UOL152H7 (Iron); Z7FF1XKL7A (cetrimonium)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171102
[Lr] Data última revisão:
171102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170324
[St] Status:MEDLINE
[do] DOI:10.2166/wst.2016.601


  7 / 2197 MEDLINE  
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[PMID]:28277083
[Au] Autor:Kaluderovic LM; Tomic ZP; Durovic-Pejcev RD; Vulic PJ; Asanin DP
[Ad] Endereço:a Institute of Soil and Melioration, Faculty of Agriculture, University of Belgrade , Belgrade , Republic of Serbia.
[Ti] Título:Influence of the organic complex concentration on adsorption of herbicide in organic modified montmorillonite.
[So] Source:J Environ Sci Health B;52(5):291-297, 2017 May 04.
[Is] ISSN:1532-4109
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:This study was undertaken to determine the impact of the organic complex concentration on the adsorption of herbicide (acetochlor) at the surface of the organic modified montmorillonite. In this work, natural montmorillonite from Bogovina (Boljevac municipality, Serbia) was used for organic modification. Cation-exchange capacity of this montmorillonite was determined using a methylene blue method (86 mmol/100 g of clay). Montmorillonite has been modified first with NaCl and then with hexadecyltrimethylammonium bromide (HDTMA-bromide) organic complex. Saturation of cation exchange capacity (CEC) was 50%, 100%, and 150%. Changes in the properties of the inorganic and organic montmorillonite have been examined using the X-ray diffraction, Fourier transform infrared spectroscopy, and batch equilibrium method. Montmorillonite modified with HDTMA-bromide demonstrated higher uptake of the herbicide, compared to the inorganic montmorillonite. Comparing the values Freundlich coefficients in batch equilibrium method, it can be seen that the adsorption of acetochlor decreased in the series: 0.5 CEC HM > 1 CEC HM > 1.5 CEC HM > NaM.
[Mh] Termos MeSH primário: Bentonita/química
Herbicidas/química
Toluidinas/química
[Mh] Termos MeSH secundário: Adsorção
Silicatos de Alumínio/química
Compostos de Cetrimônio/química
Poluentes Ambientais/química
Azul de Metileno/química
Sérvia
Espectroscopia de Infravermelho com Transformada de Fourier
Difração de Raios X
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Aluminum Silicates); 0 (Cetrimonium Compounds); 0 (Environmental Pollutants); 0 (Herbicides); 0 (Toluidines); 1302-78-9 (Bentonite); 1302-87-0 (clay); 8L08WMO94K (acetochlor); T42P99266K (Methylene Blue); Z7FF1XKL7A (cetrimonium)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170918
[Lr] Data última revisão:
170918
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170310
[St] Status:MEDLINE
[do] DOI:10.1080/03601234.2017.1281636


  8 / 2197 MEDLINE  
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[PMID]:28277034
[Au] Autor:Cangiotti M; Battistelli M; Salucci S; Falcieri E; Mattioli M; Giordani M; Ottaviani MF
[Ad] Endereço:a Department of Pure and Applied Sciences , University of Urbino , Urbino , Italy.
[Ti] Título:Electron paramagnetic resonance and transmission electron microscopy study of the interactions between asbestiform zeolite fibers and model membranes.
[So] Source:J Toxicol Environ Health A;80(3):171-187, 2017.
[Is] ISSN:1528-7394
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Different asbestiform zeolite fibers of the erionite (termed GF1 and MD8, demonstrated carcinogenic) and offretite (termed BV12, suspected carcinogenic) families were investigated by analyzing the electron paramagnetic resonance (EPR) spectra of selected surfactant spin probes and transmission electron microscopy (TEM) images in the presence of model membranes-cetyltrimethylammonium (CTAB) micelles, egg-lecithin liposomes, and dimyristoylphosphatidylcholine (DMPC) liposomes. This was undertaken to obtain information on interactions occurring at a molecular level between fibers and membranes which correlate with entrance of fibers into the membrane model or location of the fibers at the external or internal membrane interfaces. For CTAB micelles, all fibers were able to enter the micelles, but the hair-like structure and chemical surface characteristics of GF1 modified the micelle structure toward a bilayer-like organization, while MD8 and BV12, being shorter fibers and with a high density of surface interacting groups, partially destroyed the micelles. For liposomes, GF1 fibers partially penetrated the core solution, but DMPC liposomes showed increasing rigidity and organization of the bilayer. Conversely, for MD8 and BV12, the fibers did not cross the membrane demonstrating a smaller membrane structure perturbation. Scolecite fibers (termed SC1), used for comparison, presented poor interactions with the model membranes. The carcinogenicity of the zeolites, as postulated in the series SC1
[Mh] Termos MeSH primário: Compostos de Cetrimônio/química
Dimiristoilfosfatidilcolina/química
Lipossomos/química
Micelas
Zeolitas/química
[Mh] Termos MeSH secundário: Espectroscopia de Ressonância de Spin Eletrônica
Membranas Artificiais
Microscopia Eletrônica de Transmissão
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cetrimonium Compounds); 0 (Liposomes); 0 (Membranes, Artificial); 0 (Micelles); 12510-42-8 (erionite); 1318-02-1 (Zeolites); U86ZGC74V5 (Dimyristoylphosphatidylcholine); Z7FF1XKL7A (cetrimonium)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170615
[Lr] Data última revisão:
170615
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170310
[St] Status:MEDLINE
[do] DOI:10.1080/15287394.2016.1275901


  9 / 2197 MEDLINE  
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[PMID]:28235720
[Au] Autor:Su X; Wang J; Zhang J; Yuwen L; Zhang Q; Dang M; Tao J; Ma X; Wang S; Teng Z
[Ad] Endereço:Key Laboratory for Organic Electronics & Information Displays and Institute of Advanced Materials, Nanjing University of Posts and Telecommunications (NUPT), Nanjing 210046, PR China.
[Ti] Título:Synthesis of sandwich-like molybdenum sulfide/mesoporous organosilica nanosheets for photo-thermal conversion and stimuli-responsive drug release.
[So] Source:J Colloid Interface Sci;496:261-266, 2017 06 15.
[Is] ISSN:1095-7103
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Sandwich-like molybdenum sulfide/mesoporous organosilica nanosheets (denoted as MoS @MOS) have been prepared for the first time via direct growth of ethane-bridged mesostructured organosilica on MoS nanosheets by using cetyltrimethylammonium bromide (CTAB) as structure directing agent. The obtained MoS @MOS nanosheets possess well-defined sandwich-like structure, high surface area (∼920cm /g), uniform pore size (∼4.2nm), large pore volume (∼1.41cm g ). In vitro cytotoxicity assessments demonstrate that the MoS @MOS nanosheets have excellent biocompatibility. Owing to the encapsulation of the MoS , the obtained MoS @MOS nanosheets have photo-thermal conversion capability and photo-thermally controlled drug release property. These properties make the MoS @MOS nanosheets promising for biomedical applications.
[Mh] Termos MeSH primário: Dissulfetos/química
Molibdênio/química
Nanoestruturas/química
Compostos de Organossilício/química
[Mh] Termos MeSH secundário: Antineoplásicos/química
Antineoplásicos/farmacologia
Materiais Biocompatíveis/química
Sobrevivência Celular/efeitos dos fármacos
Compostos de Cetrimônio/química
Dissulfetos/toxicidade
Doxorrubicina/química
Doxorrubicina/farmacologia
Sistemas de Liberação de Medicamentos
Liberação Controlada de Fármacos
Temperatura Alta
Seres Humanos
Lasers
Células MCF-7
Molibdênio/toxicidade
Nanoestruturas/efeitos da radiação
Nanoestruturas/toxicidade
Compostos de Organossilício/toxicidade
Tamanho da Partícula
Porosidade
Propriedades de Superfície
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (Biocompatible Materials); 0 (Cetrimonium Compounds); 0 (Disulfides); 0 (Organosilicon Compounds); 80168379AG (Doxorubicin); 81AH48963U (Molybdenum); Z7FF1XKL7A (cetrimonium); ZC8B4P503V (molybdenum disulfide)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171013
[Lr] Data última revisão:
171013
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170226
[St] Status:MEDLINE


  10 / 2197 MEDLINE  
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[PMID]:28232090
[Au] Autor:Lopez BR; Hernandez JP; Bashan Y; de-Bashan LE
[Ad] Endereço:Environmental Microbiology Group, Northwestern Center for Biological Research (CIBNOR), Calle IPN 195, La Paz, B.C.S. 23096, Mexico; The Bashan Institute of Science, 1730 Post Oak Court, Auburn, AL 36830, USA.
[Ti] Título:Immobilization of microalgae cells in alginate facilitates isolation of DNA and RNA.
[So] Source:J Microbiol Methods;135:96-104, 2017 Apr.
[Is] ISSN:1872-8359
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Isolation of nucleic acids from Chlorella is difficult, given the chemically complex nature of their cell walls and variable production of metabolites. Immobilization of microalgae in polymers adds additional difficulty. Here, we modified, amended, and standardized methods for isolation of nucleic acids and compared the yield of DNA and RNA from free-living and encapsulated microalgae C. sorokiniana. Isolation of nucleic acids from immobilized cells required two steps in dissolving the alginate matrix, releasing the cells, and mechanical disruption with glass beads. For DNA extraction, we used modified versions of a commercial kit along with the hexadecyltrimethylammonium bromide (CTAB) method. For RNA extraction, we used the commercial TRI reagent procedure and the CTAB-dithiotreitol method. Quantity and quality of nucleic acids in extracts varied with growth conditions, isolation procedures, and time of incubation of the original culture. There were consistently higher amounts of DNA and RNA in extracts from immobilized cells. Quantitatively, the modified procedure with the commercial Promega kit was the most reliable procedure for isolating DNA and a modified commercial TRI reagent procedure was the choice for isolating RNA. All four procedures eliminated proteins efficiently and had low levels of contamination from residual polysaccharides from the matrices and/or metabolites naturally produced by the microalgae. All DNA extracts under both growth conditions, time of incubation, and two isolation methods successfully amplified the 18S ribosomal RNA by PCR and quantitative reverse transcription (RT-qPCR).
[Mh] Termos MeSH primário: Alginatos/química
Células Imobilizadas
DNA/isolamento & purificação
Microalgas/química
Microalgas/genética
RNA/isolamento & purificação
[Mh] Termos MeSH secundário: Compostos de Cetrimônio/química
Chlorella/química
Chlorella/genética
Chlorella/crescimento & desenvolvimento
Chlorella/metabolismo
DNA/química
Vidro
Ácido Glucurônico/química
Ácidos Hexurônicos/química
Microalgas/crescimento & desenvolvimento
Microalgas/metabolismo
Reação em Cadeia da Polimerase/métodos
Polissacarídeos/metabolismo
RNA/química
RNA Ribossômico 18S/análise
RNA Ribossômico 18S/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alginates); 0 (Cetrimonium Compounds); 0 (Hexuronic Acids); 0 (Polysaccharides); 0 (RNA, Ribosomal, 18S); 63231-63-0 (RNA); 8A5D83Q4RW (Glucuronic Acid); 8C3Z4148WZ (alginic acid); 9007-49-2 (DNA); Z7FF1XKL7A (cetrimonium)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171002
[Lr] Data última revisão:
171002
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170225
[St] Status:MEDLINE



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