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[PMID]:28470123
[Au] Autor:Dojo K; Yamaguchi Y; Fustin JM; Doi M; Kobayashi M; Okamura H
[Ad] Endereço:Department of Systems Biology, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo-ku, Kyoto, Japan.
[Ti] Título:Carbachol Induces Phase-dependent Phase Shifts of Per1 Transcription Rhythms in Cultured Suprachiasmatic Nucleus Slices.
[So] Source:J Biol Rhythms;32(2):101-108, 2017 Apr.
[Is] ISSN:1552-4531
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Among nonphotic stimulants, a classic cholinergic agonist, carbachol, is known to have a strong and unique phase-resetting effect on the circadian clock: Intracerebroventricular carbachol treatment causes phase delays during the subjective early night and phase advances in the subjective late night, but the effects of this drug on the suprachiasmatic nucleus (SCN) in vivo and in vitro are still controversial. In the present study, we succeeded in reproducing the biphasic phase-shifting effect of carbachol on clock gene expression in organotypic SCN slices prepared from mice carrying a Per1-promoter fused luciferase gene ( Per1-luc). Since this biphasic effect of carbachol in Per1-luc SCN was prevented by atropine but not by mecamylamine, we concluded that these phase shifts were muscarinic receptor-dependent. Next, we analyzed the expression of muscarinic receptors in the SCN by in situ hybridization and found that M3 and M4 subtypes were expressed in SCN cells. These signals appeared neonatally and reached adult levels at postnatal day 10. Together, these findings suggest that carbachol has a phase-dependent phase-shifting effect on the SCN clock through muscarinic receptor subtypes expressed in the SCN.
[Mh] Termos MeSH primário: Carbacol/farmacologia
Agonistas Colinérgicos/farmacologia
Ritmo Circadiano/efeitos dos fármacos
Proteínas Circadianas Period/genética
Núcleo Supraquiasmático/efeitos dos fármacos
Núcleo Supraquiasmático/fisiologia
Transcrição Genética
[Mh] Termos MeSH secundário: Animais
Animais Recém-Nascidos
Atropina/farmacologia
Relógios Circadianos/efeitos dos fármacos
Expressão Gênica
Luciferases/genética
Mecamilamina/farmacologia
Camundongos
Atividade Motora
Antagonistas Muscarínicos/farmacologia
Antagonistas Nicotínicos/farmacologia
Técnicas de Cultura de Órgãos
Regiões Promotoras Genéticas
Receptores Muscarínicos/genética
Receptores Muscarínicos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cholinergic Agonists); 0 (Muscarinic Antagonists); 0 (Nicotinic Antagonists); 0 (Per1 protein, mouse); 0 (Period Circadian Proteins); 0 (Receptors, Muscarinic); 6EE945D3OK (Mecamylamine); 7C0697DR9I (Atropine); 8Y164V895Y (Carbachol); EC 1.13.12.- (Luciferases)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180126
[Lr] Data última revisão:
180126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1177/0748730417691205


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[PMID]:28985235
[Au] Autor:Tilunaite A; Croft W; Russell N; Bellamy TC; Thul R
[Ad] Endereço:School of Mathematical Sciences, University of Nottingham, Nottingham, England, United Kingdom.
[Ti] Título:A Bayesian approach to modelling heterogeneous calcium responses in cell populations.
[So] Source:PLoS Comput Biol;13(10):e1005794, 2017 Oct.
[Is] ISSN:1553-7358
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Calcium responses have been observed as spikes of the whole-cell calcium concentration in numerous cell types and are essential for translating extracellular stimuli into cellular responses. While there are several suggestions for how this encoding is achieved, we still lack a comprehensive theory. To achieve this goal it is necessary to reliably predict the temporal evolution of calcium spike sequences for a given stimulus. Here, we propose a modelling framework that allows us to quantitatively describe the timing of calcium spikes. Using a Bayesian approach, we show that Gaussian processes model calcium spike rates with high fidelity and perform better than standard tools such as peri-stimulus time histograms and kernel smoothing. We employ our modelling concept to analyse calcium spike sequences from dynamically-stimulated HEK293T cells. Under these conditions, different cells often experience diverse stimulus time courses, which is a situation likely to occur in vivo. This single cell variability and the concomitant small number of calcium spikes per cell pose a significant modelling challenge, but we demonstrate that Gaussian processes can successfully describe calcium spike rates in these circumstances. Our results therefore pave the way towards a statistical description of heterogeneous calcium oscillations in a dynamic environment.
[Mh] Termos MeSH primário: Potenciais de Ação/fisiologia
Teorema de Bayes
Sinalização do Cálcio/fisiologia
Cálcio/metabolismo
Modelos Biológicos
[Mh] Termos MeSH secundário: Potenciais de Ação/efeitos dos fármacos
Sinalização do Cálcio/efeitos dos fármacos
Carbacol/farmacologia
Agonistas Colinérgicos/farmacologia
Células HEK293
Seres Humanos
Análise de Célula Única/métodos
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cholinergic Agonists); 8Y164V895Y (Carbachol); SY7Q814VUP (Calcium)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171007
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pcbi.1005794


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[PMID]:28893640
[Au] Autor:Turan I; Ozacmak HS; Ozacmak VH; Barut F; Arasli M
[Ad] Endereço:Department of Physiology, Faculty of Medicine, Bulent Ecevit University, Zonguldak, Turkey. Electronic address: inci.turan@beun.edu.tr.
[Ti] Título:Agmatine attenuates intestinal ischemia and reperfusion injury by reducing oxidative stress and inflammatory reaction in rats.
[So] Source:Life Sci;189:23-28, 2017 Nov 15.
[Is] ISSN:1879-0631
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:AIMS: Oxidative stress and inflammatory response are major factors causing several tissue injuries in intestinal ischemia and reperfusion (I/R). Agmatine has been reported to attenuate I/R injury of various organs. The present study aims to analyze the possible protective effects of agmatine on intestinal I/R injury in rats. MAIN METHODS: Four groups were designed: sham control, agmatine-treated control, I/R control, and agmatine-treated I/R groups. IR injury of small intestine was induced by the occlusion of the superior mesenteric artery for half an hour to be followed by a 3-hour-long reperfusion. Agmatine (10mg/kg) was administered intraperitoneally before reperfusion period. After 180min of reperfusion period, the contractile responses to both carbachol and potassium chloride (KCl) were subsequently examined in an isolated-organ bath. Malondialdehyde (MDA), reduced glutathione (GSH), and the activity of myeloperoxidase (MPO) were measured in intestinal tissue. Plasma cytokine levels were determined. The expression of the intestinal inducible nitric oxide synthase (iNOS) was also assessed by immunohistochemistry. KEY FINDINGS: The treatment with agmatine appeared to be significantly effective in reducing the MDA content and MPO activity besides restoring the content of GSH. The treatment also attenuated the histological injury. The increases in the I/R induced expressions of iNOS, IFN-γ, and IL-1α were brought back to the sham control levels by the treatment as well. SIGNIFICANCE: Our findings indicate that the agmatine pretreatment may ameliorate reperfusion induced injury in small intestine mainly due to reducing inflammatory response and oxidative stress.
[Mh] Termos MeSH primário: Agmatina/farmacologia
Inflamação/tratamento farmacológico
Intestino Delgado/efeitos dos fármacos
Estresse Oxidativo/efeitos dos fármacos
Traumatismo por Reperfusão/tratamento farmacológico
[Mh] Termos MeSH secundário: Animais
Carbacol/farmacologia
Modelos Animais de Doenças
Inflamação/patologia
Intestino Delgado/patologia
Masculino
Malondialdeído/metabolismo
Peroxidase/metabolismo
Cloreto de Potássio/farmacologia
Ratos
Ratos Wistar
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
4Y8F71G49Q (Malondialdehyde); 660YQ98I10 (Potassium Chloride); 70J407ZL5Q (Agmatine); 8Y164V895Y (Carbachol); EC 1.11.1.7 (Peroxidase)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171023
[Lr] Data última revisão:
171023
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170913
[St] Status:MEDLINE


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Antunes, Edson
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[PMID]:28487215
[Au] Autor:Bonilla-Becerra SM; de Oliveira MG; Calmasini FB; Rojas-Moscoso JA; Zanesco A; Antunes E
[Ad] Endereço:Department of Pharmacology, Faculty of Medical Sciences, University of Campinas (UNICAMP), Campinas, São Paulo, Brazil.
[Ti] Título:Micturition dysfunction in four-month old ovariectomized rats: Effects of testosterone replacement.
[So] Source:Life Sci;179:120-129, 2017 Jun 15.
[Is] ISSN:1879-0631
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:AIMS: Androgen deficiency has been implicated in urological complications of postmenopausal women. This study examined the effects of testosterone replacements on the lower urinary tract dysfunction in 4-month old ovariectomized (OVX) rats. MAIN METHODS: Sprague-Dawley female rats were OVX bilaterally. Three months later, rats received single intramuscular injections of testosterone undecanoate. Cystometric study, and bladder and urethra smooth muscle reactivities were evaluated. KEY FINDINGS: Ovariectomy reduced by 65% (p<0.05) the serum testosterone levels. Testosterone replacement at 5mg/kg restored serum hormone levels to baseline, whereas 10mg/kg produced 14-fold higher testosterone levels. OVX rats exhibited significant increases of body weight, perigonadal fat and blood pressure, and reduced uterus weight, but none of these parameters were changed by testosterone replacements. OVX rats exhibited micturition dysfunction characterized by increases of basal pressure, threshold pressure, voiding frequency and post-voiding pressure. In addition, the bladder contractions induced by electrical-field stimulation (EFS) and carbachol were significantly reduced, whereas angiotensin II-induced urethral contractions were significantly increased in OVX rats. Testosterone replacement at 10mg/kg (but not at 5mg/kg) dose fully normalized the in vivo micturition dysfunction, as well as the in vitro bladder and urethral alterations. Testosterone (10mg/kg) also significantly potentiated the bladder relaxations induced by the ß -adrenoceptor agonist mirabegron. The protective effects of testosterone were not modified by concomitant treatment with the aromatase inhibitor letrozole (2.5mg/kg, 4weeks). SIGNIFICANCE: The improvement of micturition dysfunction by testosterone replacement suggests that androgen therapy might be of therapeutic benefit for urological complications associated with post-menopause.
[Mh] Termos MeSH primário: Androgênios/administração & dosagem
Músculo Liso/efeitos dos fármacos
Pós-Menopausa
Testosterona/análogos & derivados
Transtornos Urinários/tratamento farmacológico
[Mh] Termos MeSH secundário: Acetanilidas/farmacologia
Androgênios/farmacologia
Angiotensina II/farmacologia
Animais
Carbacol/farmacologia
Modelos Animais de Doenças
Relação Dose-Resposta a Droga
Feminino
Injeções Intramusculares
Músculo Liso/metabolismo
Nitrilos/farmacologia
Ovariectomia
Ratos
Ratos Sprague-Dawley
Testosterona/administração & dosagem
Testosterona/farmacologia
Tiazóis/farmacologia
Triazóis/farmacologia
Uretra/efeitos dos fármacos
Uretra/metabolismo
Transtornos Urinários/etiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acetanilides); 0 (Androgens); 0 (Nitriles); 0 (Thiazoles); 0 (Triazoles); 11128-99-7 (Angiotensin II); 3XMK78S47O (Testosterone); 7LKK855W8I (letrozole); 8Y164V895Y (Carbachol); H16A5VCT9C (testosterone undecanoate); MVR3JL3B2V (mirabegron)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170629
[Lr] Data última revisão:
170629
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170511
[St] Status:MEDLINE


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[PMID]:28446461
[Au] Autor:Im YJ; Lee JK; Lee SH; Oh SJ; Park K
[Ad] Endereço:Department of Urology, College of Medicine, Seoul National University, Seoul, Korea.
[Ti] Título:Developmental changes in contractile responses to cholinergic stimuli: role of calcium sensitization and related pathways.
[So] Source:Am J Physiol Renal Physiol;313(2):F370-F377, 2017 Aug 01.
[Is] ISSN:1522-1466
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:This study was performed to analyze the developmental changes in bladder response to cholinergic stimulation in detail, highlighting calcium sensitization (CS) and its related pathways. Rats were divided into three groups in accordance with reported time of developmental milestones (newborns, ; youngsters, ; and grown-ups, ). Following cholinergic stimulation (carbachol, 5 µM), the contractile response to detrusor was analyzed with respect to three phases (initial phasic, tonic, and superimposed phasic contractions). Contractile responses were analyzed by their dynamic and kinetic aspects. The responses were further compared in varying external calcium concentrations and in the presence of inhibitors of protein kinase C (PKC) and Rho kinase (ROCK), which are involved in CS. The responses of newborns contrasted with the others by their short and brisk initial phasic contractions, prominent tonic contractions, and delayed participation of irregular superimposed phasic contractions. With development, phasic contractions became prominent, and tonic contractions diminished. These developmental changes in phasic contractions were reproduced when exposed to increasing calcium concentrations. Application of specific inhibitors and molecular phasic analysis revealed that PKC was functional in tonic contractions of the newborns, whereas ROCK took over its role with development. Within a few days of birth, rats' bladders experienced drastic changes in contractile mechanisms. This included dominance of phasic contractions over tonic contractions due to increased calcium dependence and the maturational shift of the calcium sensitivity mechanism from PKC to ROCK.
[Mh] Termos MeSH primário: Compostos de Cálcio/farmacologia
Sinalização do Cálcio/efeitos dos fármacos
Carbacol/farmacologia
Agonistas Colinérgicos/farmacologia
Contração Muscular/efeitos dos fármacos
Músculo Liso/efeitos dos fármacos
Bexiga Urinária/efeitos dos fármacos
[Mh] Termos MeSH secundário: Fatores Etários
Animais
Animais Recém-Nascidos
Relação Dose-Resposta a Droga
Técnicas In Vitro
Cinética
Músculo Liso/crescimento & desenvolvimento
Cadeias Leves de Miosina/metabolismo
Fosfoproteínas Fosfatases/metabolismo
Fosforilação
Proteína Quinase C/metabolismo
Proteína Fosfatase 1/metabolismo
Ratos Sprague-Dawley
Bexiga Urinária/crescimento & desenvolvimento
Quinases Associadas a rho/metabolismo
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Calcium Compounds); 0 (Cholinergic Agonists); 0 (Myosin Light Chains); 8Y164V895Y (Carbachol); EC 2.7.11.1 (rho-Associated Kinases); EC 2.7.11.13 (Protein Kinase C); EC 3.1.3.16 (Phosphoprotein Phosphatases); EC 3.1.3.16 (Ppp1r12a protein, rat); EC 3.1.3.16 (Protein Phosphatase 1)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170918
[Lr] Data última revisão:
170918
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170428
[St] Status:MEDLINE
[do] DOI:10.1152/ajprenal.00597.2016


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[PMID]:28410965
[Au] Autor:Tsuneoka Y; Irie M; Tanaka Y; Sugimoto T; Kobayashi Y; Kusakabe T; Kato K; Hamaguchi S; Namekata I; Tanaka H
[Ad] Endereço:Department of Pharmacology, Faculty of Pharmaceutical Sciences, Toho University, Funabashi, Chiba 274-8510, Japan; Laboratory of Pharmacology, Faculty of Pharmaceutical Science, Tokyo University of Sciences, Noda, Chiba 278-8510, Japan.
[Ti] Título:Permissive role of reduced inwardly-rectifying potassium current density in the automaticity of the guinea pig pulmonary vein myocardium.
[So] Source:J Pharmacol Sci;133(4):195-202, 2017 Apr.
[Is] ISSN:1347-8648
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:The electrophysiological properties underlying the automaticity of the guinea pig pulmonary vein myocardium were studied. About 30% of the isolated pulmonary vein tissue preparations showed spontaneous electrical activity, as shown by glass microelectrode recordings from their myocardial layer. The remaining quiescent preparations had a resting membrane potential less negative than that in the left atria. Blockade of the acetylcholine activated potassium current (I ) by tertiapin induced a depolarizing shift of the resting membrane potential and automatic electrical activity in the pulmonary vein, but not in the atria. The tertiapin-induced electrical activity, as well as the spontaneous activity, was inhibited by the application of carbachol or by chelation of intracellular Ca by BAPTA. The isolated pulmonary vein cardiomyocytes had an I density similar to that of the atrial cardiomyocytes, but a lower density of the inwardly-rectifying potassium current (I ). Spontaneous Ca transients were observed in about 30% of the isolated pulmonary vein cardiomyocytes, but not in atrial cardiomyocytes. The Ca transients in the pulmonary vein cardiomyocytes were induced by tertiapin and inhibited by carbachol. These results indicate that the pulmonary vein cardiomyocytes have a reduced density of the inwardly-rectifying potassium current, which plays a permissive role in their intracellular Ca -dependent automaticity.
[Mh] Termos MeSH primário: Cálcio/metabolismo
Miócitos Cardíacos/metabolismo
Potássio/metabolismo
Potássio/fisiologia
Veias Pulmonares/metabolismo
Veias Pulmonares/fisiologia
[Mh] Termos MeSH secundário: Acetilcolina/antagonistas & inibidores
Acetilcolina/farmacologia
Potenciais de Ação/efeitos dos fármacos
Animais
Venenos de Abelha/antagonistas & inibidores
Venenos de Abelha/farmacologia
Carbacol/farmacologia
Ácido Egtázico/análogos & derivados
Ácido Egtázico/farmacologia
Fenômenos Eletrofisiológicos/efeitos dos fármacos
Cobaias
Técnicas In Vitro
Potenciais da Membrana/efeitos dos fármacos
Microscopia Confocal
Bloqueadores dos Canais de Potássio/farmacologia
Veias Pulmonares/citologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bee Venoms); 0 (Potassium Channel Blockers); 3A7MX9B7E8 (tertiapin); 526U7A2651 (Egtazic Acid); 8Y164V895Y (Carbachol); K22DDW77C0 (1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid); N9YNS0M02X (Acetylcholine); RWP5GA015D (Potassium); SY7Q814VUP (Calcium)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171013
[Lr] Data última revisão:
171013
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170416
[St] Status:MEDLINE


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[PMID]:28400257
[Au] Autor:Johnson NW; Özkan M; Burgess AP; Prokic EJ; Wafford KA; O'Neill MJ; Greenhill SD; Stanford IM; Woodhall GL
[Ad] Endereço:Aston Brain Centre, Aston University, School of Life and Health Sciences, Birmingham, B4 7ET, United Kingdom.
[Ti] Título:Phase-amplitude coupled persistent theta and gamma oscillations in rat primary motor cortex in vitro.
[So] Source:Neuropharmacology;119:141-156, 2017 Jun.
[Is] ISSN:1873-7064
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In vivo, theta (4-7 Hz) and gamma (30-80 Hz) neuronal network oscillations are known to coexist and display phase-amplitude coupling (PAC). However, in vitro, these oscillations have for many years been studied in isolation. Using an improved brain slice preparation technique we have, using co-application of carbachol (10 µM) and kainic acid (150 nM), elicited simultaneous theta (6.6 ± 0.1 Hz) and gamma (36.6 ± 0.4 Hz) oscillations in rodent primary motor cortex (M1). Each oscillation showed greatest power in layer V. Using a variety of time series analyses we detected significant cross-frequency coupling in 74% of slice preparations. Differences were observed in the pharmacological profile of each oscillation. Thus, gamma oscillations were reduced by the GABA receptor antagonists, gabazine (250 nM and 2 µM), and picrotoxin (50 µM) and augmented by AMPA receptor antagonism with SYM2206 (20 µM). In contrast, theta oscillatory power was increased by gabazine, picrotoxin and SYM2206. GABA receptor blockade with CGP55845 (5 µM) increased both theta and gamma power, and similar effects were seen with diazepam, zolpidem, MK801 and a series of metabotropic glutamate receptor antagonists. Oscillatory activity at both frequencies was reduced by the gap junction blocker carbenoxolone (200 µM) and by atropine (5 µM). These data show theta and gamma oscillations in layer V of rat M1 in vitro are cross-frequency coupled, and are mechanistically distinct. The development of an in vitro model of phase-amplitude coupled oscillations will facilitate further mechanistic investigation of the generation and modulation of coupled activity in mammalian cortex.
[Mh] Termos MeSH primário: Ritmo Gama/fisiologia
Córtex Motor/fisiologia
Ritmo Teta/fisiologia
[Mh] Termos MeSH secundário: Potenciais de Ação/efeitos dos fármacos
Potenciais de Ação/fisiologia
Animais
Animais Recém-Nascidos
Carbacol/farmacologia
Agonistas Colinérgicos/farmacologia
Relação Dose-Resposta a Droga
Agonistas de Aminoácidos Excitatórios
Ritmo Gama/efeitos dos fármacos
Técnicas In Vitro
Ácido Caínico/farmacologia
Masculino
Córtex Motor/efeitos dos fármacos
Neurotransmissores/farmacologia
Ratos
Ratos Wistar
Receptores de GABA/metabolismo
Ritmo Teta/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cholinergic Agonists); 0 (Excitatory Amino Acid Agonists); 0 (Neurotransmitter Agents); 0 (Receptors, GABA); 8Y164V895Y (Carbachol); SIV03811UC (Kainic Acid)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170831
[Lr] Data última revisão:
170831
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170413
[St] Status:MEDLINE


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[PMID]:28223240
[Au] Autor:Lévesque M; Cataldi M; Chen LY; Hamidi S; Avoli M
[Ad] Endereço:Montreal Neurological Institute and Department of Neurology & Neurosurgery, McGill University, 3801 University Street, Montréal, PQ H3A 2B4, Canada.
[Ti] Título:Carbachol-induced network oscillations in an in vitro limbic system brain slice.
[So] Source:Neuroscience;348:153-164, 2017 Apr 21.
[Is] ISSN:1873-7544
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We employed simultaneous field potential recordings from CA3, subiculum and entorhinal cortex in an in vitro brain slice preparation to understand the involvement of these limbic areas in the generation of the field potential oscillations that are induced by bath application of the muscarinic receptor agonist carbachol. Regularly spaced oscillations that mainly presented at theta frequency range (5-12Hz) occurred synchronously in all three structures in the presence of carbachol. These oscillations, which disappeared when slices were perfused with pirenzepine or with glutamatergic receptor antagonists, were categorized as short (<4s) and long (>4s) with short events oscillating at higher frequencies than long events. Field oscillations were highly synchronized between regions and latency analysis revealed that they often initiated in the entorhinal cortex later than in the other two structures. Blocking GABA receptors modified the activity patterns of both short and long oscillations and decreased their coherence in the theta frequency range. Finally, blocking KCC2 activity disclosed a pattern of recurrent short oscillations. Our results suggest that in the presence of carbachol both subiculum and CA3 most often drive theta generators in the entorhinal cortex and that these oscillations are influenced but not abolished by altering GABA receptor signaling.
[Mh] Termos MeSH primário: Região CA3 Hipocampal/efeitos dos fármacos
Carbacol/farmacologia
Agonistas Colinérgicos/farmacologia
Córtex Entorrinal/efeitos dos fármacos
Hipocampo/efeitos dos fármacos
Rede Nervosa/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Masculino
Antagonistas Muscarínicos/farmacologia
Neurônios/efeitos dos fármacos
Pirenzepina/farmacologia
Ratos
Ratos Sprague-Dawley
Ritmo Teta/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cholinergic Agonists); 0 (Muscarinic Antagonists); 3G0285N20N (Pirenzepine); 8Y164V895Y (Carbachol)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170223
[St] Status:MEDLINE


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[PMID]:28183233
[Au] Autor:Mahmood H; Chaudhry MA; Masood Z; Saeed MA; Adnan S
[Ad] Endereço:a Faculty of Pharmacy , The University of Lahore , Lahore , Pakistan.
[Ti] Título:A mechanistic evaluation of the traditional uses of Nepeta ruderalis in gastrointestinal and airway disorders.
[So] Source:Pharm Biol;55(1):1017-1021, 2017 Dec.
[Is] ISSN:1744-5116
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:CONTEXT: Nepeta ruderalis Buch.-Ham. (Lamiaceae), locally known as Badranj Boya, is an aromatic herb used traditionally as an antispasmodic, antidiarrhoeal, and anti-asthamatic remedy. OBJECTIVE: Aqueous methanolic extract of N. ruderalis was studied to investigate its traditional uses. MATERIALS AND METHODS: Study was conducted from September 2015 to February 2016. In vitro spasmolytic and broncho-relaxant activity of crude extract of N. ruderalis (whole plant) was evaluated at 0.01-10 mg/mL final bath concentration in isolated rabbit jejunum and tracheal tissues, using PowerLab data acquisition system (Transonic Systems Inc., Ithaca, NY). In vivo antidiarrhoeal activity was evaluated in castor oil-induced diarrhoeal mice at the dose of 300 and 500 mg of crude extract orally. RESULTS: Crude extract of N. ruderalis completely relaxed spontaneously contracting, high K (80 mM) and carbachol (1 µM) induced contracted jejunum with an EC value of 5.85 (5.45-6.27), 4.0 (3.80-4.23) and 2.86 (2.48-3.29), similar to verapamil. Nr.Cr relaxed high K and carbachol induced contractions, at 5 and 10 mg/mL with an EC value of 2.37 (2.11-2.67) and 3.26 (2.9-3.67), respectively, and also displaced calcium concentration-response curves toward right at 0.1 and 0.3 mg/mL. Nr.Cr exhibited antidiarrhoeal protection at a dose of 300 and 500 mg/kg, similar to verapamil, whereas no acute toxicity signs were seen up to 5 g/kg in healthy mice. DISCUSSION AND CONCLUSION: Results suggest the presence of spasmolytic and broncho-relaxant effects in the crude extract of N. ruderalis, possibly mediated through calcium channel-blocking activity, providing the pharmacological basis for its traditional uses in gastrointestinal and airway disorders.
[Mh] Termos MeSH primário: Jejuno/efeitos dos fármacos
Nepeta
Fitoterapia
Extratos Vegetais/farmacologia
Traqueia/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Antidiarreicos/farmacologia
Cálcio/metabolismo
Bloqueadores dos Canais de Cálcio/farmacologia
Carbacol/farmacologia
Jejuno/fisiologia
Camundongos
Extratos Vegetais/toxicidade
Coelhos
Traqueia/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antidiarrheals); 0 (Calcium Channel Blockers); 0 (Plant Extracts); 8Y164V895Y (Carbachol); SY7Q814VUP (Calcium)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170303
[Lr] Data última revisão:
170303
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170211
[St] Status:MEDLINE
[do] DOI:10.1080/13880209.2017.1285325


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[PMID]:28138904
[Au] Autor:Kaya E; Kibar Y; Yilmaz S; Ebiloglu T; Ozcan A; Seyrek M; Yildiz O; Ulusoy KG
[Ad] Endereço:Gulhane Military Medical Academy, Ankara, Turkey. drenginkaya@yahoo.com.
[Ti] Título:The histopathological and pharmacodynamic effects of intradetrusor decorin injected in a rabbit partial bladder outlet obstruction model.
[So] Source:Int Urol Nephrol;49(4):607-614, 2017 Apr.
[Is] ISSN:1573-2584
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:PURPOSE: To evaluate whether or not the bladder function can be protected by supporting the detrusor with decorin levels during the fibrotic process. METHODS: Forty-two male rabbits were divided into three main groups, partial bladder outlet obstruction (pBOO) group, pBOO + intradetrusor decorin-injected (IDI) group and control group. Both pBOO and pBOO + IDI groups were divided into three subgroups according to the killing schedule. Histopathological, immunohistochemical and pharmacodynamics studies were performed for the evaluation of fibrotic process and tissue characteristics. RESULTS: Histopathological evaluation revealed statistically significant high fibrosis levels for both pBOO and pBOO + IDI groups when compared with control. Strikingly the antifibrotic effect of decorin was significant on 2nd, 4th and 8th week and increased as time passed. Immunohistochemical analysis was revealed high expressions of anti-TGF-ß1 and decorin levels in all pBOO + IDI groups. Pharmacodynamical results were also revealed better contraction responses in favor of 2nd, 4th and 8th week groups of pBOO + IDI groups, when compared with pBOO groups. In addition, the contraction responses against the depolarizer agent KCl were increased in the three decorin-administrated groups. CONCLUSION: Our study demonstrates the antifibrotic effects of decorin on bladder fibrosis. Strikingly, this antifibrotic effect is shown in histopathological, immunohistochemical and pharmacodynamics studies. Although further studies are warranted to make more decisive inferences regarding its clinical use, our study has the proper pride to be the first step of this time course.
[Mh] Termos MeSH primário: Decorina/farmacologia
Músculo Liso/efeitos dos fármacos
Obstrução do Colo da Bexiga Urinária/tratamento farmacológico
Obstrução do Colo da Bexiga Urinária/patologia
Bexiga Urinária/efeitos dos fármacos
Bexiga Urinária/patologia
[Mh] Termos MeSH secundário: Animais
Carbacol/farmacologia
Decorina/análise
Decorina/uso terapêutico
Modelos Animais de Doenças
Estimulação Elétrica
Fibrose
Injeções Intramusculares
Masculino
Antagonistas Muscarínicos/farmacologia
Contração Muscular/efeitos dos fármacos
Músculo Liso/fisiopatologia
Cloreto de Potássio/farmacologia
Coelhos
Fator de Crescimento Transformador beta1/análise
Bexiga Urinária/química
Bexiga Urinária/fisiopatologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Decorin); 0 (Muscarinic Antagonists); 0 (Transforming Growth Factor beta1); 660YQ98I10 (Potassium Chloride); 8Y164V895Y (Carbachol)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171102
[Lr] Data última revisão:
171102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170201
[St] Status:MEDLINE
[do] DOI:10.1007/s11255-017-1518-x



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