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Pesquisa : D02.092.877.883.333.800.030 [Categoria DeCS]
Referências encontradas : 275 [refinar]
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[PMID]:28299407
[Au] Autor:Gumpu MB; Nesakumar N; Nagarajan S; Ramanujam S; Krishnan UM; Babu KJ; Rayappan JB
[Ad] Endereço:Nanosensors Lab, SASTRA University, Thanjavur, Tamil Nadu, 613 401, India.
[Ti] Título:Design and Development of Acetylthiocholine Electrochemical Biosensor Based on Zinc Oxide-Cerium Oxide Nanohybrid Modified Platinum Electrode.
[So] Source:Bull Environ Contam Toxicol;98(5):662-671, 2017 May.
[Is] ISSN:1432-0800
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Acetylcholinesterase (AChE) enzyme has been predominantly used for the detection of pesticides and metal ions. But, these sensors respond to pesticides as well as metal ions at certain concentration, which results in poor selectivity. Hence in this work, the amount of thiocholine produced during AChE inhibition has been estimated to detect the residual activity of AChE enzyme in-turn to enhance the efficiency of the biosensor. In this context, Pt/ZnO-CeO /AChE/Chitosan based biosensor has been developed for sensitive voltammetric quantification of thiocholine in AChE. The sensor exhibited enhanced electron transfer rate, good conductivity and biocompatibility. Both the intrinsic and extrinsic parameters were simultaneously optimized using second order polynomial regression to get the best conditions for ATCh determination. Under optimized experimental conditions, the redox peak current was linear over the concentration range of 0.1-1.5 mM with detection and quantification limit of 0.05 and 0.15 µM respectively and the sensitivity of 1.47 µA mM .
[Mh] Termos MeSH primário: Técnicas Biossensoriais/instrumentação
Cério/química
Desenho de Equipamento
Nanoestruturas/química
Platina/química
Óxido de Zinco/química
[Mh] Termos MeSH secundário: Acetilcolinesterase/metabolismo
Acetiltiocolina/análise
Quitosana/química
Inibidores da Colinesterase/metabolismo
Eletrodos
Concentração de Íons de Hidrogênio
Limite de Detecção
Praguicidas/análise
Sensibilidade e Especificidade
Tiocolina/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cholinesterase Inhibitors); 0 (Pesticides); 30K4522N6T (Cerium); 4468-05-7 (Acetylthiocholine); 49DFR088MY (Platinum); 619G5K328Y (ceric oxide); 625-00-3 (Thiocholine); 9012-76-4 (Chitosan); EC 3.1.1.7 (Acetylcholinesterase); SOI2LOH54Z (Zinc Oxide)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170609
[Lr] Data última revisão:
170609
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170317
[St] Status:MEDLINE
[do] DOI:10.1007/s00128-017-2045-2


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[PMID]:28182991
[Au] Autor:Hernández B; Houzé P; Pflüger F; Kruglik SG; Ghomi M
[Ad] Endereço:CNRS UMR 7369, Laboratoire Matrice Extracellulaire et Dynamique Cellulaire, Université de Reims Champagne Ardenne, UFR Sciences Exactes et Naturelles, Moulin de la Housse, 51687 Reims Cedex 2, France; Groupe de Biophysique Moléculaire, Sorbonne Paris Cité, Université Paris 13, UFR Santé-Médecine-Bio
[Ti] Título:Raman scattering-based multiconformational analysis for probing the structural differences between acetylcholine and acetylthiocholine.
[So] Source:J Pharm Biomed Anal;138:54-62, 2017 May 10.
[Is] ISSN:1873-264X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Acetylcholine is the first discovered neurotransmitter that has received a great attention regarding its capability of binding to several cellular targets. The chemical composition of acetylcholine, including a positively charged trimethylammonium and a carbonyl group, as well as its conformational flexibility was pointed out as the key factors in the stabilization of its interactions. Here, the possibilities offered by a Raman scattering-based multiconformatioal analysis to access the most stable conformers of acetylcholine, is discussed. To control the validity of this protocol, acetylcholine and one of its closely structured analogues, acetylthiocholine, were simultaneously analyzed. Solution Raman spectra revealed distinct and well resolved strong markers for each molecule. Density functional theory calculations were consistent with the fact that the energy order of the low energy conformers is considerably affected by the acyloxy oxygen→sulfur atom substitution. Raman spectra were calculated on the basis of the thermal average of the spectra arising from the low energy conformers. It has been evidenced that the carbonyl and trimethylammonium groups are the most favorable hydration sites in aqueous environment. Taking into account the large gap between the carbonyl bond-stretch and aliphatic bending bands, Raman spectra also allowed separation of the HOH bending vibrations arising from the bound and bulk water molecules.
[Mh] Termos MeSH primário: Acetilcolina/química
Acetiltiocolina/química
Análise Espectral Raman/métodos
[Mh] Termos MeSH secundário: Conformação Molecular
Soluções/química
Vibração
Água/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Solutions); 059QF0KO0R (Water); 4468-05-7 (Acetylthiocholine); N9YNS0M02X (Acetylcholine)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170628
[Lr] Data última revisão:
170628
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170210
[St] Status:MEDLINE


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[PMID]:27507601
[Au] Autor:Estévez J; Selva V; Benabent M; Mangas I; Sogorb MÁ; Vilanova E
[Ad] Endereço:University "Miguel Hernández", Institute of Bioengineering, Unit of Toxicology, Elche, Spain. Electronic address: jorge.estevez@umh.es.
[Ti] Título:Acetylcholine-hydrolyzing activities in soluble brain fraction: Characterization with reversible and irreversible inhibitors.
[So] Source:Chem Biol Interact;259(Pt B):374-381, 2016 Nov 25.
[Is] ISSN:1872-7786
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:Some effects of organophosphorus compounds (OPs) esters cannot be explained through actions on currently recognized targets acetylcholinesterase or neuropathy target esterase (NTE). In soluble chicken brain fraction, three components (Eα, Eß and Eγ) of pheny lvalerate esterase activity (PVase) were kinetically discriminated and their relationship with acetylcholine-hydrolyzing activity (cholinesterase activity) were studied in previous works. In this work, four enzymatic components (CS1, CS2, CS3 and CS4) of cholinesterase activity have been discriminated in soluble fraction, according to their sensitivity to irreversible inhibitors mipafox, paraoxon, PMSF and iso-OMPA and to reversible inhibitors ethopropazine and BW284C51. Cholinesterase component CS1 can be related to the Eα component of PVase activity and identified as butyrylcholinesterase (BuChE). No association and similarities can be stablished among the other PVase component (Eß and Eγ) with the other cholinesterase components (CS2, CS3, CS4). The kinetic analysis has allowed us to stablish a method for discriminating the enzymatic component based on a simple test with two inhibitors. It can be used as biomarker in toxicological studies and for monitoring these cholinesterase components during isolation and molecular identification processes, which will allow OP toxicity to be understood by a multi-target approach.
[Mh] Termos MeSH primário: Acetilcolina/metabolismo
Acetilcolinesterase/metabolismo
Encéfalo/enzimologia
Inibidores da Colinesterase/farmacologia
[Mh] Termos MeSH secundário: Acetiltiocolina/metabolismo
Animais
Hidrolases de Éster Carboxílico/antagonistas & inibidores
Hidrolases de Éster Carboxílico/metabolismo
Galinhas
Hidrólise/efeitos dos fármacos
Fenotiazinas/farmacologia
Fosforamidas/farmacologia
Solubilidade
Frações Subcelulares/enzimologia
Fatores de Tempo
Compostos de Tosil/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cholinesterase Inhibitors); 0 (Phenothiazines); 0 (Phosphoramides); 0 (Tosyl Compounds); 4468-05-7 (Acetylthiocholine); 455-16-3 (4-toluenesulfonyl fluoride); 7WI4P02YN1 (profenamine); EC 3.1.1.- (Carboxylic Ester Hydrolases); EC 3.1.1.- (neurotoxic esterase); EC 3.1.1.7 (Acetylcholinesterase); N9YNS0M02X (Acetylcholine)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170130
[Lr] Data última revisão:
170130
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160811
[St] Status:MEDLINE


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[PMID]:26920268
[Au] Autor:Hou J; Dong G; Tian Z; Lu J; Wang Q; Ai S; Wang M
[Ad] Endereço:College of Chemistry and Material Science, Shandong Agricultural University, Taian, Shandong 271018, PR China; College of Food Science and Engineering, Shandong Agricultural University, Taian, Shandong 271018, PR China.
[Ti] Título:A sensitive fluorescent sensor for selective determination of dichlorvos based on the recovered fluorescence of carbon dots-Cu(II) system.
[So] Source:Food Chem;202:81-7, 2016 Jul 01.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In this paper, a simple and sensitive fluorescent sensor for dichlorvos was first constructed based on carbon dots-Cu(II) system. These carbon dots were obtained by simple hydrothermal reaction of feather. The fluorescence of these carbon dots can be selectively quenched by Cu(2+) ion. When acetylcholinesterase and acetylthiocholine were introduced into the system, thiocholine came into being, which can react with Cu(2+) ion and restore the fluorescence of the system. The reaction mechanism between Cu(2+) ion and thiocholine was confirmed by X-ray photoelectron spectroscopy. As one kind of acetylcholinesterase inhibitor, organophosphorus pesticides can be detected based on this sensing system. As an example of organophosphorus pesticides, dichlorvos was detected with a linear range of 6.0×10(-9)-6.0×10(-8)M. This sensing system has been successfully used for the analysis of cabbage and fruit juice samples.
[Mh] Termos MeSH primário: Diclorvós/análise
Praguicidas/análise
Espectroscopia Fotoeletrônica/métodos
[Mh] Termos MeSH secundário: Acetilcolinesterase/química
Acetiltiocolina/química
Brassica/química
Carbono/química
Inibidores da Colinesterase/análise
Cobre/química
Fluorescência
Análise de Alimentos
Contaminação de Alimentos/análise
Sucos de Frutas e Vegetais/análise
Sensibilidade e Especificidade
Tiocolina/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Cholinesterase Inhibitors); 0 (Pesticides); 4468-05-7 (Acetylthiocholine); 625-00-3 (Thiocholine); 7440-44-0 (Carbon); 789U1901C5 (Copper); 7U370BPS14 (Dichlorvos); EC 3.1.1.7 (Acetylcholinesterase)
[Em] Mês de entrada:1611
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160228
[St] Status:MEDLINE


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[PMID]:26841098
[Au] Autor:Zhang N; Ma ZY; Ruan YF; Zhao WW; Xu JJ; Chen HY
[Ad] Endereço:State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University , Nanjing, Jiangsu 210023, China.
[Ti] Título:Simultaneous Photoelectrochemical Immunoassay of Dual Cardiac Markers Using Specific Enzyme Tags: A Proof of Principle for Multiplexed Bioanalysis.
[So] Source:Anal Chem;88(4):1990-4, 2016 Feb 16.
[Is] ISSN:1520-6882
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In this Letter, on the basis of the CdS quantum dots functionalized TiO2 nanotubes electrode, we proposed a simultaneous photoelectrochemical (PEC) immunoassay of dual cardiac markers using specific enzyme tags of alkaline phosphatase (ALP) and acetylcholine esterase (AChE). ALP and AChE were integrated into the PEC system through the sandwich immunobinding and could specifically catalyze the hydrolysis of ascorbic acid 2-phosphate (AAP) or the acetylthiocholine (ATC) to in situ generate ascorbic acid (AA) or thiocholine (TC) for sacrificial electron donating. These two enzymes were thus used to differentiate the signals of two cardiac targets in connection with the sandwich immunorecognition and PEC responses to the corresponding electron donors. This strategy demonstrates a proof of principle for the successful integration of dual enzyme tags with PEC immunoassay that can potentially provide a general format for multiplexed PEC bioanalysis.
[Mh] Termos MeSH primário: Acetilcolinesterase/metabolismo
Fosfatase Alcalina/metabolismo
Técnicas de Química Analítica/métodos
Técnicas Eletroquímicas
Imunoensaio
[Mh] Termos MeSH secundário: Acetiltiocolina/metabolismo
Anticorpos Imobilizados/química
Anticorpos Imobilizados/imunologia
Ácido Ascórbico/análogos & derivados
Ácido Ascórbico/metabolismo
Proteína C-Reativa/análise
Eletrodos
Seres Humanos
Nanotubos/química
Pontos Quânticos/química
Titânio/química
Troponina I/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antibodies, Immobilized); 0 (Troponin I); 15FIX9V2JP (titanium dioxide); 23313-12-4 (ascorbate-2-phosphate); 4468-05-7 (Acetylthiocholine); 9007-41-4 (C-Reactive Protein); D1JT611TNE (Titanium); EC 3.1.1.7 (Acetylcholinesterase); EC 3.1.3.1 (Alkaline Phosphatase); PQ6CK8PD0R (Ascorbic Acid)
[Em] Mês de entrada:1609
[Cu] Atualização por classe:160216
[Lr] Data última revisão:
160216
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160204
[St] Status:MEDLINE
[do] DOI:10.1021/acs.analchem.5b04579


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[PMID]:26547618
[Au] Autor:Song Y; Chen J; Sun M; Gong C; Shen Y; Song Y; Wang L
[Ad] Endereço:Key Laboratory of Functional Small Organic Molecule, Ministry of Education, College of Chemistry and Chemical Engineering, Jiangxi Normal University, 99 Ziyang Road, Nanchang 330022, China.
[Ti] Título:A simple electrochemical biosensor based on AuNPs/MPS/Au electrode sensing layer for monitoring carbamate pesticides in real samples.
[So] Source:J Hazard Mater;304:103-9, 2016 Mar 05.
[Is] ISSN:1873-3336
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A simple electrochemical biosensor for quantitative determination of carbamate pesticide was developed based on a sensing interface of citrate-capped gold nanoparticles (AuNPs)/(3-mercaptopropyl)-trimethoxysilane (MPS)/gold electrode (Au). The biosensor was fabricated by firstly assembling three-dimensional (3D) MPS networks on Au electrode and subsequently assembling citrate-capped AuNPs on 3D MPS network via AuS bond. The interface of AuNPs/MPS/Au was negatively charged originating from the citrate coated on AuNPs that would repulse the negatively charged ferricyanide ([Fe(CN)6](3-/4-)) to produce a negative response. In the presence of acetylcholinesterase (AChE) and acetylthiocholine (ATCl), the AChE catalyzes the hydrolysis of ATCl into positively charged thiocholine which would replace the citrate on AuNPs through the strong AuS bond and convert the negative charged surface to be positively charged. The resulted positively charged AuNPs/MPS/Au then attracted the [Fe(CN)6](3-/4-) to produce a positive response. Based on the inhibition of carbamate pesticides on the activity of AChE, the pesticide could be quantitatively determined at a very low potential. The linear range was from 0.003 to 2.00 µM. The sensing platform was also proved to be suitable for carbamate pesticides detection in practical sample.
[Mh] Termos MeSH primário: Técnicas Biossensoriais
Carbamatos/análise
Praguicidas/análise
[Mh] Termos MeSH secundário: Acetilcolinesterase/química
Acetiltiocolina/química
Carbamatos/química
Citratos/química
Técnicas Eletroquímicas
Eletrodos
Ouro/química
Nanopartículas Metálicas/química
Praguicidas/química
Silanos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Carbamates); 0 (Citrates); 0 (Pesticides); 0 (Silanes); 4468-05-7 (Acetylthiocholine); 7440-57-5 (Gold); 8CB1M08OIW ((3-mercaptopropyl)trimethoxysilane); EC 3.1.1.7 (Acetylcholinesterase)
[Em] Mês de entrada:1609
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151109
[St] Status:MEDLINE


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[PMID]:26757125
[Au] Autor:Vetchý D; Pitschmann V; Vetchá M; Kasparovský T; Matejovský L
[Ad] Endereço:Department of Pharmaceutics, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic.
[Ti] Título:Preparation and evaluation of carriers for detection of cholinesterase inhibitors.
[So] Source:Neuro Endocrinol Lett;36 Suppl 1:95-9, 2015.
[Is] ISSN:0172-780X
[Cp] País de publicação:Sweden
[La] Idioma:eng
[Ab] Resumo:OBJECTIVES: The aim of the study was to use methods of pharmaceutical technology, and prepare carriers in the form of pellets suitable as a filling of detection tubes for enzymatic detection of cholinesterase inhibitors. The enzymatic detection was based on enzymatic hydrolysis of acetylthiocholine iodide and the subsequent colour reaction of its hydrolysis product with Ellman's reagent. The suitable carriers should be in the form of white, regular and sufficiently mechanically resistant particles of about 1 mm allowing it to capture the enzyme during the impregnation process and ensuring its high activity for enzymatic detection. METHODS: Carriers consisting of microcrystalline cellulose, lactose, povidone, and sodium carboxymethyl cellulose were prepared using extrusion-spheronization method under three different drying conditions in either a hot air oven or a microwave oven. Subsequently, the carriers were impregnated with acetylcholinesterase and their size, shape, mechanical resistance, bulk, tapped and pycnometric density, Hausner ratio, intraparticular and total tapped porosity, and activity were measured and recorded. RESULTS: In this procedure, carriers with different physical parameters and different acetylcholinesterase activity were evaluated. It was found that higher acetylcholinesterase activity was associated not only with a higher intraparticular porosity but also with more regular particles characterized by high sphericity and low total tapped porosity. CONCLUSION: This unique finding is important for the preparation of detection tubes based on enzymatic detection which is still irreplaceable especially in the field of detection and analysis of super-toxic cholinesterase inhibitors.
[Mh] Termos MeSH primário: Carboximetilcelulose Sódica/análise
Celulose/análise
Inibidores da Colinesterase/metabolismo
Lactose/análise
Povidona/análise
[Mh] Termos MeSH secundário: Acetiltiocolina/análogos & derivados
Acetiltiocolina/metabolismo
Ácido Ditionitrobenzoico
Teste de Materiais
Porosidade
Reagentes de Sulfidrila
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Cholinesterase Inhibitors); 0 (Sulfhydryl Reagents); 0 (acetylthiocholine iodide); 4468-05-7 (Acetylthiocholine); 9004-34-6 (Cellulose); 9BZQ3U62JX (Dithionitrobenzoic Acid); FZ989GH94E (Povidone); J2B2A4N98G (Lactose); K679OBS311 (Carboxymethylcellulose Sodium); OP1R32D61U (microcrystalline cellulose)
[Em] Mês de entrada:1604
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160113
[St] Status:MEDLINE


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[PMID]:26217956
[Au] Autor:Lu L; Xia Y
[Ad] Endereço:Key Laboratory of Functional Molecular Solids, Ministry of Education, College of Chemistry and Materials Science, Anhui Normal University, Wuhu, 241000, China.
[Ti] Título:Enzymatic reaction modulated gold nanorod end-to-end self-assembly for ultrahigh sensitively colorimetric sensing of cholinesterase and organophosphate pesticides in human blood.
[So] Source:Anal Chem;87(16):8584-91, 2015 Aug 18.
[Is] ISSN:1520-6882
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We present herein the first reported self-assembly modulation of gold nanorods (AuNRs) by enzymatic reaction, which is further employed for colorimetric assays of cholinesterase (ChE) and organophosphate pesticides (OPs) in human blood. ChE catalyzes its substrate (acetylthiocholine) and produces thiocholine and acetate acid. The resulting thiols then react with the tips of the AuNRs by S-Au conjunction and prevent subsequent cysteine-induced AuNR end-to-end (EE) self-assembly. Correspondingly, the AuNR surface plasmon resonance is regulated, which results in a distinctly ratiometric signal output. Under optimal conditions, the linear range is 0.042 to 8.4 µU/mL, and the detection limit is as low as 0.018 µU/mL. As ChE is incubated with OPs, the enzymatic activity is inhibited. So, the cysteine-induced assembly is observed again. On the basis of this principle, OPs can be well determined ranging from 0.12 to 40 pM with a 0.039 pM detection limit. To our knowledge, the present quasi pU/mL level sensitivity for ChE and the quasi femtomolar level sensitivity for OPs are at least 500 and 7000 times lower than those of previous colorimetric methods, respectively. The ultrahigh sensitivity results from (1) the rational choice of anisotropic AuNRs as building blocks and reporters and (2) the specific structure of the enzymatic thiocholine. Because of ultrahigh sensitivity, serum samples are allowed to be extremely diluted in the assay. Accordingly, various nonspecific interactions, even from glutathione/cysteine, are well avoided. So, both ChE and OPs in human blood can be directly assayed without any prepurification, indicating the simplicity and practical promise of the proposed method.
[Mh] Termos MeSH primário: Colinesterases/metabolismo
Colorimetria/métodos
Ouro/química
Nanotubos/química
Compostos Organofosforados/sangue
Praguicidas/sangue
[Mh] Termos MeSH secundário: Acetiltiocolina/química
Acetiltiocolina/metabolismo
Colinesterases/sangue
Colinesterases/química
Cisteína/química
Seres Humanos
Limite de Detecção
Especificidade por Substrato
Ressonância de Plasmônio de Superfície
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Organophosphorus Compounds); 0 (Pesticides); 4468-05-7 (Acetylthiocholine); 7440-57-5 (Gold); EC 3.1.1.8 (Cholinesterases); K848JZ4886 (Cysteine)
[Em] Mês de entrada:1602
[Cu] Atualização por classe:150818
[Lr] Data última revisão:
150818
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150729
[St] Status:MEDLINE
[do] DOI:10.1021/acs.analchem.5b02516


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[PMID]:26165279
[Au] Autor:Wang J; Satake T; Suzuki H
[Ad] Endereço:Graduate School of Life and Environmental Sciences, University of Tsukuba.
[Ti] Título:Microfluidic Device for Coulometric Detection of Organophosphate Pesticides.
[So] Source:Anal Sci;31(7):591-5, 2015.
[Is] ISSN:1348-2246
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:A microdevice for coulometric detection of organophosphate pesticides (OPs) was fabricated based on the measurement of the inhibition of an enzyme, acetylcholinesterase (AChE), by OPs. Thiocholine (TCh) produced in the enzymatic reaction of AChE with acetylthiocholine (ATCh) as a substrate was oxidized on a microelectrode array formed in a main flow channel. Volumes of plugs of necessary solutions were measured using a structure consisting of a row of rhombuses formed in an auxiliary flow channel. The plugs were merged and solution components were mixed at a T-junction formed with the main and auxiliary flow channels. A linear relationship was confirmed between the generated charge and the logarithm of the OP (malathion) concentration in a concentration range between 10(-6) and 10(-3) M with a correlation coefficient of 0.951. The lower limit of detection was 412 nM.
[Mh] Termos MeSH primário: Técnicas Biossensoriais/instrumentação
Inibidores da Colinesterase/análise
Dispositivos Lab-On-A-Chip
Malation/análise
[Mh] Termos MeSH secundário: Acetilcolinesterase/metabolismo
Acetiltiocolina/metabolismo
Animais
Inibidores da Colinesterase/química
Inibidores da Colinesterase/farmacologia
Electrophorus
Malation/química
Malation/farmacologia
Microeletrodos
Oxirredução
Tiocolina/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cholinesterase Inhibitors); 4468-05-7 (Acetylthiocholine); 625-00-3 (Thiocholine); EC 3.1.1.7 (Acetylcholinesterase); U5N7SU872W (Malathion)
[Em] Mês de entrada:1604
[Cu] Atualização por classe:150713
[Lr] Data última revisão:
150713
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150714
[St] Status:MEDLINE
[do] DOI:10.2116/analsci.31.591


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[PMID]:25660508
[Au] Autor:Ma K; Lu L; Qi Z; Feng J; Zhuo C; Zhang Y
[Ad] Endereço:Key Laboratory of Applied Surface and Colloid Chemistry of Ministry of Education, Xi'an 710062, PR China; Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710062, PR China.
[Ti] Título:In situ induced metal-enhanced fluorescence: a new strategy for biosensing the total acetylcholinesterase activity in sub-microliter human whole blood.
[So] Source:Biosens Bioelectron;68:648-53, 2015 Jun 15.
[Is] ISSN:1873-4235
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) activities (i.e., total AChE) in human blood are biomarkers for theranostic monitoring of organophosphate neurotoxin-poisoned patients. We developed an ultra-sensitive method to detect the total AChE activity in sub-microliter human whole blood based on in situ induced metal-enhanced fluorescence (MEF). Both AChE and BChE can catalyze the hydrolysis of the acetylthiocholine (ATCh) substrate and produce positively-charged thiocholine (TCh). TCh can reverse the negatively-charged surface of core-shell Ag@SiO2 nanoparticles (NPs). The negatively-charged fluorescent dye (8-hydroxypyrene-1,3,6-trisulfonic acid, HPTS) is then confined to the surface of Ag@SiO2 NPs and generates an enhanced fluorescence signal in situ. Changes in the surface charge of Ag@SiO2 NPs are monitored by Zeta potential, and the MEF effect is confirmed by the measurements of fluorescence time decay. AChE activity has a dynamic range of 0 U/mL to 0.005 U/mL and a detection limit of 0.05 mU/mL. The total AChE activity in the sub-microliter human whole blood could be determined; the results were further validated. Therefore, combining the AChE catalytic reaction with MEF provides a simple, ultra-sensitive, and cost-effective "in situ MEF" approach to determine the total AChE activity in human whole blood sample down to sub-microliters without matrix interferences. The strategy also allows potential usage in other tissues and other fields.
[Mh] Termos MeSH primário: Acetilcolinesterase/isolamento & purificação
Técnicas Biossensoriais
Nanopartículas Metálicas/química
[Mh] Termos MeSH secundário: Acetilcolinesterase/sangue
Acetiltiocolina/química
Acetiltiocolina/metabolismo
Catálise
Inibidores da Colinesterase/química
Fluorescência
Seres Humanos
Dióxido de Silício/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Cholinesterase Inhibitors); 4468-05-7 (Acetylthiocholine); 7631-86-9 (Silicon Dioxide); EC 3.1.1.7 (Acetylcholinesterase)
[Em] Mês de entrada:1511
[Cu] Atualização por classe:150302
[Lr] Data última revisão:
150302
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150210
[St] Status:MEDLINE



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