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[PMID]:27935961
[Au] Autor:Nally JE; Arent Z; Bayles DO; Hornsby RL; Gilmore C; Regan S; McDevitt AD; Yearsley J; Fanning S; McMahon BJ
[Ad] Endereço:Infectious Bacterial Diseases Research Unit, National Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, Ames, Iowa, United States of America.
[Ti] Título:Emerging Infectious Disease Implications of Invasive Mammalian Species: The Greater White-Toothed Shrew (Crocidura russula) Is Associated With a Novel Serovar of Pathogenic Leptospira in Ireland.
[So] Source:PLoS Negl Trop Dis;10(12):e0005174, 2016 Dec.
[Is] ISSN:1935-2735
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The greater white-toothed shrew (Crocidura russula) is an invasive mammalian species that was first recorded in Ireland in 2007. It currently occupies an area of approximately 7,600 km2 on the island. C. russula is normally distributed in Northern Africa and Western Europe, and was previously absent from the British Isles. Whilst invasive species can have dramatic and rapid impacts on faunal and floral communities, they may also be carriers of pathogens facilitating disease transmission in potentially naive populations. Pathogenic leptospires are endemic in Ireland and a significant cause of human and animal disease. From 18 trapped C. russula, 3 isolates of Leptospira were cultured. However, typing of these isolates by standard serological reference methods was negative, and suggested an, as yet, unidentified serovar. Sequence analysis of 16S ribosomal RNA and secY indicated that these novel isolates belong to Leptospira alstonii, a unique pathogenic species of which only 7 isolates have been described to date. Earlier isolations were limited geographically to China, Japan and Malaysia, and this leptospiral species had not previously been cultured from mammals. Restriction enzyme analysis (REA) further confirms the novelty of these strains since no similar patterns were observed with a reference database of leptospires. As with other pathogenic Leptospira species, these isolates contain lipL32 and do not grow in the presence of 8-azagunaine; however no evidence of disease was apparent after experimental infection of hamsters. These isolates are genetically related to L. alstonii but have a novel REA pattern; they represent a new serovar which we designate as serovar Room22. This study demonstrates that invasive mammalian species act as bridge vectors of novel zoonotic pathogens such as Leptospira.
[Mh] Termos MeSH primário: Doenças Transmissíveis Emergentes/microbiologia
Leptospira/isolamento & purificação
Leptospirose/microbiologia
Musaranhos/microbiologia
[Mh] Termos MeSH secundário: Animais
Azaguanina/farmacologia
Proteínas da Membrana Bacteriana Externa/genética
Técnicas de Tipagem Bacteriana
China/epidemiologia
Doenças Transmissíveis Emergentes/epidemiologia
Doenças Transmissíveis Emergentes/transmissão
Cricetinae
Vetores de Doenças
Seres Humanos
Espécies Introduzidas
Irlanda/epidemiologia
Japão/epidemiologia
Leptospira/classificação
Leptospira/efeitos dos fármacos
Leptospira/patogenicidade
Leptospirose/epidemiologia
Leptospirose/transmissão
Lipoproteínas/genética
Malásia/epidemiologia
Reação em Cadeia da Polimerase
RNA Ribossômico 16S
Sorogrupo
Zoonoses/epidemiologia
Zoonoses/microbiologia
Zoonoses/transmissão
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Outer Membrane Proteins); 0 (LipL32 protein, Leptospira); 0 (Lipoproteins); 0 (RNA, Ribosomal, 16S); Q150359I72 (Azaguanine)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170613
[Lr] Data última revisão:
170613
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161210
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pntd.0005174


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[PMID]:26729076
[Au] Autor:Stachelska-Wierzchowska A; Wierzchowski J; Bzowska A; Wielgus-Kutrowska B
[Ad] Endereço:Department of Physics and Biophysics, University of Varmia & Masuria in Olsztyn, 4 Oczapowskiego St., 10-719 Olsztyn, Poland. alicja.stachelska@uwm.edu.pl.
[Ti] Título:Site-Selective Ribosylation of Fluorescent Nucleobase Analogs Using Purine-Nucleoside Phosphorylase as a Catalyst: Effects of Point Mutations.
[So] Source:Molecules;21(1):E44, 2015 Dec 28.
[Is] ISSN:1420-3049
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Enzymatic ribosylation of fluorescent 8-azapurine derivatives, like 8-azaguanine and 2,6-diamino-8-azapurine, with purine-nucleoside phosphorylase (PNP) as a catalyst, leads to N9, N8, and N7-ribosides. The final proportion of the products may be modulated by point mutations in the enzyme active site. As an example, ribosylation of the latter substrate by wild-type calf PNP gives N7- and N8-ribosides, while the N243D mutant directs the ribosyl substitution at N9- and N7-positions. The same mutant allows synthesis of the fluorescent N7-ß-d-ribosyl-8-azaguanine. The mutated form of the E. coli PNP, D204N, can be utilized to obtain non-typical ribosides of 8-azaadenine and 2,6-diamino-8-azapurine as well. The N7- and N8-ribosides of the 8-azapurines can be analytically useful, as illustrated by N7-ß-d-ribosyl-2,6-diamino-8-azapurine, which is a good fluorogenic substrate for mammalian forms of PNP, including human blood PNP, while the N8-riboside is selective to the E. coli enzyme.
[Mh] Termos MeSH primário: Azaguanina/análogos & derivados
Mutação Puntual
Purina-Núcleosídeo Fosforilase/genética
[Mh] Termos MeSH secundário: Azaguanina/química
Catálise
Domínio Catalítico
Corantes Fluorescentes/química
Corantes Fluorescentes/metabolismo
Seres Humanos
Estrutura Molecular
Purina-Núcleosídeo Fosforilase/química
Purina-Núcleosídeo Fosforilase/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Fluorescent Dyes); EC 2.4.2.1 (Purine-Nucleoside Phosphorylase); Q150359I72 (Azaguanine)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160106
[St] Status:MEDLINE


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[PMID]:26320620
[Au] Autor:Dhimitruka I; Eubank TD; Gross AC; Khramtsov VV
[Ad] Endereço:Dorothy M. Davis Heart & Lung Research Institute, and Department of Internal Medicine, The Ohio State University, Columbus, OH 43210, United States.
[Ti] Título:New class of 8-aryl-7-deazaguanine cell permeable fluorescent probes.
[So] Source:Bioorg Med Chem Lett;25(20):4593-6, 2015 Oct 15.
[Is] ISSN:1464-3405
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A one step synthesis of fluorescent 8-aryl-(7-deazaguanines) has been accomplished. Probes exhibit blue to green high quantum yield fluorescence in a variety of organic and aqueous solutions, high extinction coefficients, and large Stokes shifts often above 100 nm. The probes are highly cell permeable, and exhibit stable bright fluorescence once intracellular; therefore are suited to the design of biosensors.
[Mh] Termos MeSH primário: Azaguanina/química
Azaguanina/metabolismo
Permeabilidade da Membrana Celular
Corantes Fluorescentes/química
Corantes Fluorescentes/metabolismo
[Mh] Termos MeSH secundário: Azaguanina/análogos & derivados
Azaguanina/síntese química
Linhagem Celular Tumoral
Fluorescência
Corantes Fluorescentes/síntese química
Seres Humanos
Células KB
Microscopia Confocal
Estrutura Molecular
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Fluorescent Dyes); Q150359I72 (Azaguanine)
[Em] Mês de entrada:1606
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150901
[St] Status:MEDLINE


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[PMID]:23384120
[Au] Autor:Gogia S; Puranik M
[Ad] Endereço:a National Centre for Biological Sciences , GKVK Campus, Bellary Road, Bangalore , 560065 , India .
[Ti] Título:Solution structures of purine base analogues 6-chloroguanine, 8-azaguanine and allopurinol.
[So] Source:J Biomol Struct Dyn;32(1):27-35, 2014.
[Is] ISSN:1538-0254
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Analogues of purine bases are highly relevant in the biological context and have been implicated as drug molecules for therapy against a number of diseases. Additionally, these molecules have been implicated to have a role in the prebiotic RNA world. However, experimental data on the structures of these molecules in aqueous solution is lacking. In this work, we report the ultraviolet resonance Raman spectra of 6-chloroguanine, 8-azaguanine and allopurinol, obtained with 260 nm excitation. The reported spectra have been assigned to normal modes computed from density functional theory (B3LYP/6-31G (d,p)) calculations. This work has been useful in identifying the solution-state structures of these molecules at neutral pH. We find that the guanine analogues 6-chloroguanine and 8-azaguanine exist as keto-N9H and keto-N7H tautomers in solution, respectively. On the other hand, the hypoxanthine analogue allopurinol exists as a mixture of keto-N9H and keto-N8H tautomers in solution. We predict that this work would be particularly useful in future vibrational studies where these molecules are present in complexes with their target proteins.
[Mh] Termos MeSH primário: Alopurinol/química
Azaguanina/química
Guanina/análogos & derivados
[Mh] Termos MeSH secundário: Guanina/química
Concentração de Íons de Hidrogênio
Estrutura Molecular
Análise Espectral Raman
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (6-chloroguanine); 5Z93L87A1R (Guanine); 63CZ7GJN5I (Allopurinol); Q150359I72 (Azaguanine)
[Em] Mês de entrada:1407
[Cu] Atualização por classe:131125
[Lr] Data última revisão:
131125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130207
[St] Status:MEDLINE
[do] DOI:10.1080/07391102.2012.745821


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[PMID]:24126376
[Au] Autor:Stachelska-Wierzchowska A; Wierzchowski J; Wielgus-Kutrowska B; Mikleusevic G
[Ad] Endereço:Department of Biophysics, University of Varmia & Masuria, 4 Oczapowskiego St., 10-719 Olsztyn, Poland. alicja.stachelska@uwm.edu.pl.
[Ti] Título:Enzymatic synthesis of highly fluorescent 8-azapurine ribosides using a purine nucleoside phosphorylase reverse reaction: variable ribosylation sites.
[So] Source:Molecules;18(10):12587-98, 2013 Oct 11.
[Is] ISSN:1420-3049
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Various forms of purine-nucleoside phosphorylase (PNP) were used as catalysts of enzymatic ribosylation of selected fluorescent 8-azapurines. It was found that the recombinant calf PNP catalyzes ribosylation of 2,6-diamino-8-azapurine in a phosphate-free medium, with ribose-1-phosphate as ribose donor, but the ribosylation site is predominantly N7 and N8, with the proportion of N8/N7 ribosylated products markedly dependent on the reaction conditions. Both products are fluorescent. Application of the E. coli PNP gave a mixture of N8 and N9-substituted ribosides. Fluorescence of the ribosylated 2,6-diamino-8-azapurine has been briefly characterized. The highest quantum yield, ~0.9, was obtained for N9-ß-d-riboside (λmax 365 nm), while for N8-ß-d-riboside, emitting at ~430 nm, the fluorescence quantum yield was found to be close to 0.4. Ribosylation of 8-azaguanine with calf PNP as a catalyst goes exclusively to N9. By contrast, the E. coli PNP ribosylates 8-azaGua predominantly at N9, with minor, but highly fluorescent products ribosylated at N8/N7.
[Mh] Termos MeSH primário: Azaguanina/análogos & derivados
Azaguanina/síntese química
Proteínas de Escherichia coli/química
Corantes Fluorescentes/síntese química
Purina-Núcleosídeo Fosforilase/química
[Mh] Termos MeSH secundário: Animais
Biocatálise
Bovinos
Glicosilação
Cinética
Proteínas Recombinantes/química
Ribosemonofosfatos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Escherichia coli Proteins); 0 (Fluorescent Dyes); 0 (Recombinant Proteins); 0 (Ribosemonophosphates); EC 2.4.2.1 (Purine-Nucleoside Phosphorylase); Q150359I72 (Azaguanine)
[Em] Mês de entrada:1404
[Cu] Atualização por classe:131015
[Lr] Data última revisão:
131015
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:131016
[St] Status:MEDLINE
[do] DOI:10.3390/molecules181012587


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[PMID]:23945945
[Au] Autor:Wong RW; Balachandran A; Haaland M; Stoilov P; Cochrane A
[Ad] Endereço:Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto M5S 1A8, Canada, Department of Molecular Genetics, University of Toronto, Toronto M5S 1A8, Canada and Department of Biochemistry, West Virginia University, Morgantown, WV 26506, USA.
[Ti] Título:Characterization of novel inhibitors of HIV-1 replication that function via alteration of viral RNA processing and rev function.
[So] Source:Nucleic Acids Res;41(20):9471-83, 2013 Nov.
[Is] ISSN:1362-4962
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Expression of the complete HIV-1 genome depends on the appropriate processing of viral RNA. Altering the balance of viral RNA processing impairs replication of the virus. In this report, we characterize two small molecule modulators of HIV-1 RNA processing, 8-azaguanine and 2-(2-(5-nitro-2-thienyl)vinyl)quinoline (5350150), which function by distinct mechanisms to suppress viral gene expression. Although only 8-Azaguanine dramatically decreased accumulation of HIV-1 unspliced and singly spliced RNAs and altered splice site usage, both compounds blocked Gag and Env expression without affecting production of Tat (p16) and Rev regulatory proteins. Subsequent analyses suggest that these compounds affect Rev-mediated RNA transport by different mechanisms. Both compounds induced cytoplasmic accumulation of Rev, suggesting that they function, in part, by impairing Rev function. This conclusion is supported by the determination that both drugs block the nuclear export of genomic HIV-1 RNA to the cytoplasm. Testing confirmed that these compounds suppress HIV-1 expression in T cells at doses below those previously used in humans for tumour chemotherapy. Together, our observations demonstrate that small molecules can be used to inhibit HIV-1 replication by altering another avenue of viral RNA processing, offering the potential for the development of novel therapeutics for controlling this disease.
[Mh] Termos MeSH primário: Fármacos Anti-HIV/farmacologia
Azaguanina/farmacologia
HIV-1/efeitos dos fármacos
Quinolinas/farmacologia
Processamento de RNA/efeitos dos fármacos
RNA Viral/metabolismo
Tiofenos/farmacologia
Produtos do Gene rev do Vírus da Imunodeficiência Humana/antagonistas & inibidores
[Mh] Termos MeSH secundário: Linfócitos T CD4-Positivos/virologia
Linhagem Celular
HIV-1/genética
HIV-1/fisiologia
Células HeLa
Seres Humanos
Proteínas Estruturais Virais/genética
Proteínas Estruturais Virais/metabolismo
Replicação Viral/efeitos dos fármacos
Produtos do Gene rev do Vírus da Imunodeficiência Humana/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (2-(2-(5-nitro-2-thienyl)vinyl)quinoline); 0 (Anti-HIV Agents); 0 (Quinolines); 0 (RNA, Viral); 0 (Thiophenes); 0 (Viral Structural Proteins); 0 (rev Gene Products, Human Immunodeficiency Virus); 0 (rev protein, Human Immunodeficiency Virus-1); Q150359I72 (Azaguanine)
[Em] Mês de entrada:1401
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130816
[St] Status:MEDLINE
[do] DOI:10.1093/nar/gkt727


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[PMID]:23203626
[Au] Autor:Saito M; Villanueva SY; Kawamura Y; Iida K; Tomida J; Kanemaru T; Kohno E; Miyahara S; Umeda A; Amako K; Gloriani NG; Yoshida S
[Ad] Endereço:Department of Bacteriology, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan. msaito@bact.med.kyushu-u.ac.jp
[Ti] Título:Leptospira idonii sp. nov., isolated from environmental water.
[So] Source:Int J Syst Evol Microbiol;63(Pt 7):2457-62, 2013 Jul.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Strain Eri-1(T) was isolated from a water sample on the campus of Kyushu University, Fukuoka, Japan. The motility and morphology of the isolate were similar to those of members of the genus Leptospira, but the spiral structure of the isolate was sharper under dark-field microscopy. Cells were 10.6 ± 1.3 µm long and 0.2 µm in diameter, with a wavelength of 0.9 µm and an amplitude of 0.4 µm. Strain Eri-1(T) grew in Korthof's medium at both 13 and 30 °C, and also in the presence of 8-azaguanine. 16S rRNA gene-based phylogenetic analysis placed strain Eri-1(T) within the radiation of the genus Leptospira where it formed a unique lineage within the clade of the known saprophytic species of the genus Leptospira. The strain was not pathogenic to hamsters. Strain Eri-1(T) exhibited low levels (11.2-12.6 %) of similarity by DNA-DNA hybridization to the three most closely related species of the genus Leptospira. The DNA G+C content of the genome of strain Eri-1(T) was 42.5 ± 0.1 mol%. These results suggest that strain Eri-1(T) represents a novel species of the genus Leptospira, for which the name Leptospira idonii sp. nov. is proposed. The type strain is Eri-1(T) ( = DSM 26084(T) = JCM 18486(T)).
[Mh] Termos MeSH primário: Leptospira/classificação
Filogenia
Microbiologia da Água
[Mh] Termos MeSH secundário: Animais
Azaguanina
Técnicas de Tipagem Bacteriana
Composição de Bases
Cricetinae
DNA Bacteriano/genética
Japão
Leptospira/genética
Leptospira/isolamento & purificação
Masculino
Dados de Sequência Molecular
Hibridização de Ácido Nucleico
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (RNA, Ribosomal, 16S); Q150359I72 (Azaguanine)
[Em] Mês de entrada:1310
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:121204
[St] Status:MEDLINE
[do] DOI:10.1099/ijs.0.047233-0


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[PMID]:22348536
[Au] Autor:Sarmento-Ribeiro AB; Dourado M; Paiva A; Freitas A; Silva T; Regateiro F; Oliveira CR
[Ad] Endereço:Applied Molecular Biology/Biochemistry Institute and Center of Investigation in Environment, Genetics and Oncobiology (CIMAGO), Faculty of Medicine, University of Coimbra, Coimbra, Portugal. absarmento@fmed.uc.pt
[Ti] Título:Apoptosis deregulation influences chemoresistance to azaguanine in human leukemic cell lines.
[So] Source:Cancer Invest;30(5):331-42, 2012 Jun.
[Is] ISSN:1532-4192
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The involvement of apoptosis in the cytotoxicity mediated by nucleoside analogues, namely azaguanine, and its implication in resistance are not well understood. Using human T-cell acute lymphoblastic leukemia cell lines, sensitive (CEM cells) and resistant to azaguanine (CM3 cells), we observe a decrease in the expression of proapoptotic proteins in CM3 cells, which may be related to the resistance to cell death induced by azaguanine. On the other hand, CM3 cells lack cross resistance with other anticarcinogenic drugs, suggesting that azaguanine may be used alternatively in the presence of chemoresistance. A better knowledge of the apoptotic pathways involved in leukemic cell death resistance may contribute to the development of therapeutic strategies, aimed to prevent chemotherapy resistance.
[Mh] Termos MeSH primário: Antimetabólitos Antineoplásicos/uso terapêutico
Apoptose/efeitos dos fármacos
Azaguanina/uso terapêutico
Leucemia/tratamento farmacológico
[Mh] Termos MeSH secundário: Linhagem Celular Tumoral
Resistência a Medicamentos Antineoplásicos
Seres Humanos
Imunofenotipagem
Leucemia/imunologia
Leucemia/parasitologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antimetabolites, Antineoplastic); Q150359I72 (Azaguanine)
[Em] Mês de entrada:1207
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:120222
[St] Status:MEDLINE
[do] DOI:10.3109/07357907.2012.659925


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[PMID]:21541747
[Au] Autor:Gong QL; Hu XG; Fang GY; Li XH
[Ad] Endereço:College of Chemistry and Materials Engineering, Wenzhou University, Wenzhou 325035, China.
[Ti] Título:Study of the interaction between 8-azaguanine and bovine serum albumin using optical spectroscopy and molecular modeling methods.
[So] Source:J Mol Model;18(2):493-500, 2012 Feb.
[Is] ISSN:0948-5023
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:The interaction between 8-azaguanine (8-Azan) and bovine serum albumin (BSA) in Tris-HCl buffer solutions at pH 7.4 was investigated by means of fluorescence and ultraviolet-visible (UV-Vis) spectroscopy. At 298 K and 310 K, at a wavelength of excitation (λ (ex)) of 282 nm, the fluorescence intensity decreased significantly with increasing concentrations of 8-Azan. Fluorescence static quenching was observed for BSA, which was attributed to the formation of a complex between 8-Azan and BSA during the binding reaction. This was illuminated further by the UV-Vis absorption spectra and the decomposition of the fluorescence spectra. The thermodynamic parameters ∆G, ∆H, ∆S were calculated. The results showed that the forces acting between 8-Azan and BSA were typical hydrophobic forces, and that the interaction process was spontaneous. The interaction distance r between 8-Azan and BSA, evaluated according to fluorescence resonance energy transfer theory, suggested that there is a high possibility of energy transfer from BSA to 8-Azan. Theoretical investigations based on homology modeling and molecular docking suggested that binding between 8-Azan and BSA is dominated by hydrophilic forces and hydrogen bonding. The theoretical investigations provided a good structural basis to explain the phenomenon of fluorescence quenching between 8-Azan and BSA.
[Mh] Termos MeSH primário: Azaguanina/química
Modelos Moleculares
Soroalbumina Bovina/química
[Mh] Termos MeSH secundário: Animais
Azaguanina/metabolismo
Sítios de Ligação
Bovinos
Transferência Ressonante de Energia de Fluorescência/métodos
Seres Humanos
Ligação Proteica
Estrutura Secundária de Proteína
Soroalbumina Bovina/metabolismo
Espectrometria de Fluorescência/métodos
Análise Espectral/métodos
Termodinâmica
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
27432CM55Q (Serum Albumin, Bovine); Q150359I72 (Azaguanine)
[Em] Mês de entrada:1205
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:110505
[St] Status:MEDLINE
[do] DOI:10.1007/s00894-011-1069-5


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[PMID]:21399605
[Au] Autor:Ridzlan FR; Bahaman AR; Khairani-Bejo S; Mutalib AR
[Ad] Endereço:Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia 43400 Serdang, Selangor, Malaysia.
[Ti] Título:Detection of pathogenic Leptospira from selected environment in Kelantan and Terengganu, Malaysia.
[So] Source:Trop Biomed;27(3):632-8, 2010 Dec.
[Is] ISSN:0127-5720
[Cp] País de publicação:Malaysia
[La] Idioma:eng
[Ab] Resumo:Leptospirosis is recognized as one of the important zoonotic diseases in the world including Malaysia. A total of 145 soil and water samples were collected from selected National Service Training Centres (NSTC) in Kelantan and Terengganu. The samples were inoculated into modified semisolid Ellinghausen McCullough Johnson Harris (EMJH) medium, incubated at room temperature for 1 month and examined under the dark-field microscope. Positive growth of the leptospiral isolates were then confirmed with 8-Azaguanine Test, Polymerase Chain Reaction (PCR) assay and Microscopic Agglutination Test (MAT). Fifteen cultures (10.34%) exhibited positive growths which were seen under dark field microscope whilst only 20% (3/15) were confirmed as pathogenic species. based on 8-Azaguanine Test and PCR. Serological identification of the isolates with MAT showed that hebdomadis was the dominant serovar in Terengganu. Pathogenic leptospires can be detected in Malaysian environment and this has the potential to cause an outbreak. Therefore, precautionary steps against leptospirosis should be taken by camp authorities to ensure the safety of trainees.
[Mh] Termos MeSH primário: Leptospira/isolamento & purificação
Microbiologia do Solo
Microbiologia da Água
[Mh] Termos MeSH secundário: Testes de Aglutinação
Animais
Antimetabólitos/metabolismo
Azaguanina/metabolismo
Técnicas Bacteriológicas
Meios de Cultura/química
Seres Humanos
Malásia
Microscopia
Reação em Cadeia da Polimerase
Sorotipagem
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antimetabolites); 0 (Culture Media); Q150359I72 (Azaguanine)
[Em] Mês de entrada:1108
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:110315
[St] Status:MEDLINE



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