Base de dados : MEDLINE
Pesquisa : D02.172.029 [Categoria DeCS]
Referências encontradas : 112 [refinar]
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[PMID]:27783247
[Au] Autor:El-Kacemi S; Zazou H; Oturan N; Dietze M; Hamdani M; Es-Souni M; Oturan MA
[Ad] Endereço:Electrochemical, Catalysis and Environment Lab, Science Faculty, Ibn-Zohr University, BO 8106, Dakhla City, Agadir, Morocco.
[Ti] Título:Nanostructured ZnO-TiO thin film oxide as anode material in electrooxidation of organic pollutants. Application to the removal of dye Amido black 10B from water.
[So] Source:Environ Sci Pollut Res Int;24(2):1442-1449, 2017 Jan.
[Is] ISSN:1614-7499
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Electrochemical oxidative degradation of diazo dye Amido black 10B (AB10B) as model pollutant in water has been studied using nanostructured ZnO-TiO thin films deposited on graphite felt (GrF) substrate as anode. The influence of various operating parameters, namely the current intensity, the nature and concentration of catalyst, the nature of electrode materials (anode/cathode), and the adsorption of dye and ambient light were investigated. It was found that the oxidative degradation of AB10B followed pseudo first-order kinetics. The optimal operating conditions for the degradation of 0.12 mM (74 mg L ) dye concentration and mineralization of its aqueous solution were determined as GrF-ZnO-TiO thin film anode, 100 mA current intensity, and 0.1 mM Fe (catalyst) concentration. Under these operating conditions, discoloration of AB10B solution was reached at 60 min while 6 h treatment needed for a mineralization degree of 91 %. Therefore, this study confirmed that the electrochemical process is effective for the degradation of AB10B in water using nanostructured ZnO-TiO thin film anodes.
[Mh] Termos MeSH primário: Negro de Amido/isolamento & purificação
Eletrodos
Titânio/química
Poluentes Químicos da Água/isolamento & purificação
Purificação da Água/métodos
Óxido de Zinco/química
[Mh] Termos MeSH secundário: Adsorção
Compostos Azo/química
Catálise
Grafite/química
Cinética
Naftalenossulfonatos/química
Oxirredução
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Azo Compounds); 0 (Naphthalenesulfonates); 0 (Naphthol Blue Black); 0 (Water Pollutants, Chemical); 15FIX9V2JP (titanium dioxide); 7782-42-5 (Graphite); D1JT611TNE (Titanium); SOI2LOH54Z (Zinc Oxide); SZT789770M (Amido Black)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:171104
[Lr] Data última revisão:
171104
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161027
[St] Status:MEDLINE
[do] DOI:10.1007/s11356-016-7920-6


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[PMID]:27471086
[Au] Autor:Hu P; Wang J; Huang R
[Ad] Endereço:College of Science, Northwest A&F University, Yangling, Shaanxi 712100, China.
[Ti] Título:Simultaneous removal of Cr(VI) and Amido black 10B (AB10B) from aqueous solutions using quaternized chitosan coated bentonite.
[So] Source:Int J Biol Macromol;92:694-701, 2016 Nov.
[Is] ISSN:1879-0003
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:In this study, simultaneous removal of Cr(VI) and Amido Black 10B (AB10B) using quaternized chitosan coated bentonite was investigated by a batch method. The factors affecting adsorption process such as pH, adsorbent dosage, contact time and initial concentration and the interaction of two components in binary solutions have been studied. The results showed that the antagonism effect existed between Cr(VI) and AB10B in binary systems. The equilibrium time for Cr(VI) adsorption was less than the one for AB10B adsorption. The maximum adsorption capacities of the modified bentonite, according to the Langmuir isotherm model were 847.5mg/g for AB10B and 66.6mg/g for Cr(VI) at 298K. The experimental results demonstrated that both Cr(VI) and AB10B were well described by the pseudo-second-order model. Thermodynamic study depicted that the adsorption of Cr(VI) and AB10B onto the modified bentonite was both spontaneous. The adsorption for Cr(VI) was exothermic while the one for AB10B was endoth- ermic in nature.
[Mh] Termos MeSH primário: Negro de Amido/isolamento & purificação
Bentonita/química
Quitosana/química
Cromo/isolamento & purificação
Corantes/isolamento & purificação
Poluentes Químicos da Água/isolamento & purificação
[Mh] Termos MeSH secundário: Adsorção
Seres Humanos
Concentração de Íons de Hidrogênio
Cinética
Temperatura Ambiente
Termodinâmica
Fatores de Tempo
Águas Residuais/química
Purificação da Água/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Coloring Agents); 0 (Waste Water); 0 (Water Pollutants, Chemical); 0R0008Q3JB (Chromium); 1302-78-9 (Bentonite); 18540-29-9 (chromium hexavalent ion); 9012-76-4 (Chitosan); SZT789770M (Amido Black)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170320
[Lr] Data última revisão:
170320
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160730
[St] Status:MEDLINE


  3 / 112 MEDLINE  
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[PMID]:27320396
[Au] Autor:Cadd S; Li B; Beveridge P; O Hare WT; Campbell A; Islam M
[Ad] Endereço:School of Science and Engineering, Teesside University, Borough Road, Middlesbrough, TS1 3BA, United Kingdom. Electronic address: cadd.sam@gmail.com.
[Ti] Título:A comparison of visible wavelength reflectance hyperspectral imaging and Acid Black 1 for the detection and identification of blood stained fingerprints.
[So] Source:Sci Justice;56(4):247-55, 2016 Jul.
[Is] ISSN:1355-0306
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Bloodstains are often encountered at scenes of violent crime and have significant forensic value for criminal investigations. Blood is one of the most commonly encountered types of biological evidence and is the most commonly observed fingerprint contaminant. Presumptive tests are used to test blood stain and blood stained fingerprints are targeted with chemical enhancement methods, such as acid stains, including Acid Black 1, Acid Violet 17 or Acid Yellow 7. Although these techniques successfully visualise ridge detail, they are destructive, do not confirm the presence of blood and can have a negative impact on DNA sampling. A novel application of visible wavelength hyperspectral imaging (HSI) is used for the non-contact, non-destructive detection and identification of blood stained fingerprints on white tiles both before and after wet chemical enhancement using Acid Black 1. The identification was obtained in a non-contact and non-destructive manner, based on the unique visible absorption spectrum of haemoglobin between 400 and 500nm. Results from the exploration of the selectivity of the setup to detect blood against ten other non-blood protein contaminants are also presented. A direct comparison of the effectiveness of HSI with chemical enhancement using Acid Black 1 on white tiles is also shown.
[Mh] Termos MeSH primário: Negro de Amido
Manchas de Sangue
Corantes
Dermatoglifia
Espectrometria de Fluorescência/métodos
[Mh] Termos MeSH secundário: Animais
Cavalos
Modelos Animais
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Coloring Agents); SZT789770M (Amido Black)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170113
[Lr] Data última revisão:
170113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160621
[St] Status:MEDLINE


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[PMID]:26540182
[Au] Autor:Hong S; Seo JY
[Ad] Endereço:Graduate School of Forensic Sciences, Soonchunhyang University, Asan, 336-745, Korea. Electronic address: swhong524@naver.com.
[Ti] Título:Chemical enhancement of fingermark in blood on thermal paper.
[So] Source:Forensic Sci Int;257:379-84, 2015 Dec.
[Is] ISSN:1872-6283
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:Chemical enhancement methods for fingermark in blood deposited on the surface of a thermal paper substrate were examined. The blood-sensitive reagents compared were LCV (leuco crystal violet), Amido black and Hungarian red. Fingermark in blood on the surface of thermal paper can be fixed with 2% 5-sulfosalicylic acid solution. LCV was found as an inadequate blood staining reagent because of bubbling, diffusion, and blurring on the surface of thermal paper. Hungarian red was also an inadequate blood staining reagent because excess Hungarian red on the surface of thermal paper was not washed away in the de-staining procedure. Amido black was the best staining reagent among three staining reagents compared. The maximum dilution ratio visible to the naked eye after Amido black staining was 1 in 80 for the thermally sensitive surface and 1 in 20 for the thermally non-sensitive surface.
[Mh] Termos MeSH primário: Sangue
Corantes
Dermatoglifia
Papel
[Mh] Termos MeSH secundário: Adulto
Negro de Amido
Feminino
Violeta de Genciana
Seres Humanos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Coloring Agents); 0 (leucocrystal violet); J4Z741D6O5 (Gentian Violet); SZT789770M (Amido Black)
[Em] Mês de entrada:1609
[Cu] Atualização por classe:151219
[Lr] Data última revisão:
151219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151106
[St] Status:MEDLINE


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[PMID]:26139252
[Au] Autor:Dsouza A; Scofield RH
[Ad] Endereço:Arthritis and Clinical Immunology Program, Oklahoma Medical Research Foundation, 825 NE 13th Street, Oklahoma City, OK, 73104, USA.
[Ti] Título:Protein Stains to Detect Antigen on Membranes.
[So] Source:Methods Mol Biol;1314:33-40, 2015.
[Is] ISSN:1940-6029
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Western blotting (protein blotting/electroblotting) is the gold standard in the analysis of complex protein mixtures. Electroblotting drives protein molecules from a polyacrylamide (or less commonly, of an agarose) gel to the surface of a binding membrane, thereby facilitating an increased availability of the sites with affinity for both general and specific protein reagents. The analysis of these complex protein mixtures is achieved by the detection of specific protein bands on a membrane, which in turn is made possible by the visualization of protein bands either by chemical staining or by reaction with an antibody of a conjugated ligand. Chemical methods employ staining with organic dyes, metal chelates, autoradiography, fluorescent dyes, complexing with silver, or prelabeling with fluorophores. All of these methods have differing sensitivities and quantitative determinations vary significantly. This review will describe the various protein staining methods applied to membranes after western blotting. "Detection" precedes and is a prerequisite to obtaining qualitative and quantitative data on the proteins in a sample, as much as to comparing the protein composition of different samples. "Detection" is often synonymous to staining, i.e., the reversible or irreversible binding by the proteins of a colored organic or inorganic chemical.
[Mh] Termos MeSH primário: Western Blotting/métodos
Corantes/química
Proteínas/análise
[Mh] Termos MeSH secundário: Negro de Amido/química
Animais
Compostos Azo/química
Carbono/química
Coloide de Ouro/química
Seres Humanos
Proteínas de Membrana/análise
Corantes de Rosanilina/química
Coloração e Rotulagem/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Azo Compounds); 0 (Coloring Agents); 0 (Gold Colloid); 0 (Membrane Proteins); 0 (Proteins); 0 (Rosaniline Dyes); 0 (chinese ink); 7440-44-0 (Carbon); 78642-64-5 (Coomassie blue); 9J3VQ0Y6BV (Fast Green); SZT789770M (Amido Black); T1C2UGS05F (ponceau S)
[Em] Mês de entrada:1603
[Cu] Atualização por classe:161125
[Lr] Data última revisão:
161125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150704
[St] Status:MEDLINE
[do] DOI:10.1007/978-1-4939-2718-0_5


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[PMID]:26139250
[Au] Autor:Goldring JP
[Ad] Endereço:Biochemistry, University of KwaZulu-Natal, Pietermaritzburg, PBX01, Scottsville, 3209, South Africa, GoldringD@ukzn.ac.za.
[Ti] Título:Measuring Protein Concentration on Nitrocellulose and After the Electrophoretic Transfer of Protein to Nitrocellulose.
[So] Source:Methods Mol Biol;1314:19-25, 2015.
[Is] ISSN:1940-6029
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Proteins bind to nitrocellulose membranes when applied directly or after electrophoretic transfer from polyacrylamide electrophoresis gels. Proteins can be stained for visualization with organic dyes Ponceau S, amido black, Coomassie Blue, and colloidal silver/gold and the intensity of the stain is directly proportional to the amount of protein present. Chemicals that interfere with dye/protein interactions in solution can be removed by washing the nitrocellulose after protein application. A method is described whereby protein-dye complexes attached to the nitrocellulose can be solubilized, dissolving the nitrocellulose and releasing dye into solution for detection by a spectrophotometer. The concentration of the dyes Ponceau S, amido black, and colloidal silver is proportional to the concentration of protein. Proteins transferred electrophoretically from SDS-PAGE, isoelectric focusing, or 2D gels to nitrocellulose can be stained with amido black, protein bands excised, and the bound dye detected in a spectrophotometer to quantify proteins in the individual protein bands.
[Mh] Termos MeSH primário: Colódio/química
Corantes/química
Proteínas/isolamento & purificação
Coloração e Rotulagem/métodos
[Mh] Termos MeSH secundário: Negro de Amido/química
Animais
Compostos Azo/química
Western Blotting/métodos
Eletroforese em Gel de Poliacrilamida/métodos
Coloide de Ouro/química
Seres Humanos
Focalização Isoelétrica/métodos
Membranas Artificiais
Proteínas/análise
Corantes de Rosanilina/química
Prata/química
Espectrofotometria/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Azo Compounds); 0 (Coloring Agents); 0 (Gold Colloid); 0 (Membranes, Artificial); 0 (Proteins); 0 (Rosaniline Dyes); 0 (colloidal silver); 3M4G523W1G (Silver); 78642-64-5 (Coomassie blue); 9004-70-0 (Collodion); SZT789770M (Amido Black); T1C2UGS05F (ponceau S)
[Em] Mês de entrada:1603
[Cu] Atualização por classe:161125
[Lr] Data última revisão:
161125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150704
[St] Status:MEDLINE
[do] DOI:10.1007/978-1-4939-2718-0_3


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[PMID]:25453286
[Au] Autor:Liu Q; Yang B; Zhang L; Huang R
[Ad] Endereço:College of Science, Northwest A&F University, Yangling, Shaanxi 712100, China.
[Ti] Título:Adsorption of an anionic azo dye by cross-linked chitosan/bentonite composite.
[So] Source:Int J Biol Macromol;72:1129-35, 2015 Jan.
[Is] ISSN:1879-0003
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:In this study, cross-linked chitosan (CCS)/bentonite (BT) composite was prepared by the intercalation of chitosan in bentonite and the cross-linking reaction between chitosan and glutaraldehyde. CCS/BT composite was characterized by using Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), X-ray diffraction (XRD) and thermal gravimetric analyses (TGA). Their adsorption characteristics were assessed by using an azo dye (Amido Black 10B) as a model adsorbate. The adsorption of Amido Black 10B onto the CCS/BT composite was found to be optimal at pH 2. The adsorption isotherm was well described by the Langmuir model and the maximum adsorption capacity was 323.6 mg/g at 293K and pH 2. Amido Black 10B adsorption kinetics followed a pseudo-second-order kinetic model. The calculated thermodynamic parameters showed that the adsorption of Amido Black 10B by CCS/BT composite was spontaneous and endothermic in nature.
[Mh] Termos MeSH primário: Negro de Amido/isolamento & purificação
Bentonita/química
Quitosana/química
Corantes/isolamento & purificação
Reagentes para Ligações Cruzadas/química
[Mh] Termos MeSH secundário: Adsorção
Ânions
Concentração de Íons de Hidrogênio
Microscopia Eletrônica de Varredura
Soluções
Espectroscopia de Infravermelho com Transformada de Fourier
Temperatura Ambiente
Termogravimetria
Difração de Raios X
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anions); 0 (Coloring Agents); 0 (Cross-Linking Reagents); 0 (Solutions); 1302-78-9 (Bentonite); 9012-76-4 (Chitosan); SZT789770M (Amido Black)
[Em] Mês de entrada:1508
[Cu] Atualização por classe:141208
[Lr] Data última revisão:
141208
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141203
[St] Status:MEDLINE


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[PMID]:25439949
[Au] Autor:Wang Y; Xia G; Wu C; Sun J; Song R; Huang W
[Ad] Endereço:College of Chemistry and Chemical Engineering, University of Chinese Academy of Sciences, Beijing 100049, China.
[Ti] Título:Porous chitosan doped with graphene oxide as highly effective adsorbent for methyl orange and amido black 10B.
[So] Source:Carbohydr Polym;115:686-93, 2015 Jan 22.
[Is] ISSN:1879-1344
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In the current study, porous chitosan aerogels doped with small amount of graphene oxide (CSGO aerogels) with high porosity (97.96-98.78%), extraordinarily high water absorption (5848-8917%) and low density (0.021-0.035 g cm(-3)) were prepared and used as adsorbents for two azo dyes methyl orange (MO) and amido black 10B (AB10B). The adsorption behavior of these CSGO aerogels and some influence factors such as pH value, graphene oxide (GO) loading, concentration of pollutants, as well as adsorption kinetics were studied. Specifically, the adsorption capacity for MO is 686.89 mg g(-1), the highest comparing with other publication results, and it is 573.47 mg g(-1) for AB10B. Since they are biodegradable, non-toxic, efficient, low-cost and easy to prepare, we believe that these porous CSGO aerogels will be a promising candidate for dye removal.
[Mh] Termos MeSH primário: Negro de Amido/química
Compostos Azo/química
Quitosana/química
Corantes/química
Grafite/química
Poluentes Químicos da Água/química
[Mh] Termos MeSH secundário: Adsorção
Concentração de Íons de Hidrogênio
Porosidade
Purificação da Água
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Azo Compounds); 0 (Coloring Agents); 0 (Water Pollutants, Chemical); 6B4TC34456 (methyl orange); 7782-42-5 (Graphite); 9012-76-4 (Chitosan); SZT789770M (Amido Black)
[Em] Mês de entrada:1511
[Cu] Atualização por classe:161125
[Lr] Data última revisão:
161125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141203
[St] Status:MEDLINE


  9 / 112 MEDLINE  
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[PMID]:25277676
[Au] Autor:Kaur S; Kaur A
[Ad] Endereço:Department of Zoology, Guru Nanak Dev University, Amritsar, Punjab 143005, India.
[Ti] Título:Variability in antioxidant/detoxification enzymes of Labeo rohita exposed to an azo dye, acid black (AB).
[So] Source:Comp Biochem Physiol C Toxicol Pharmacol;167:108-16, 2015 Jan.
[Is] ISSN:1532-0456
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The aim of the present study was to evaluate effect of a highly toxic azo dye, acid black (AB) (CI: 20470, 96 h LC50=10 mg/L) on the biochemical responses of Labeo rohita. Antioxidant/detoxification enzymes such as glutathione-S-transferase (GST), glutathione reductase (GR), glutathione peroxidase (GPx), catalase (CAT), superoxide dismutase (SOD), acetylcholinesterase (AChE), lactate dehydrogenase (LDH), succinate dehydrogenase (SDH), acid phosphatase (AcP), alkaline phosphatase (AKP), alanine transaminase (ALT) and aspartate transaminase (AST) were determined in liver, kidney, gill, muscle and brain of L. rohita after 96 h exposure to 6 mg/L (LC10), 8 mg/L (LC30) and 10 mg/L (LC50) of dye with an aim to find out the target tissue and biomarker enzyme for AB. The fish were then kept for a recovery period of 90 days, and activity of the selected enzymes was determined at the end of this period. Present dye altered the activities of all these enzymes in the selected tissues of the experimental fish in a dose-dependent manner. SOD was the maximally affected enzyme, and liver was the most affected tissue. The results indicate that AB is very toxic to L. rohita as there was a significant effect of even 6 mg/L dose of the dye and the toxicity prolonged for a long time because the fish was not able to recover from the stress even 90 days after the exposure. The study suggests that SOD can be used as a biomarker enzyme and liver is the target tissue for AB.
[Mh] Termos MeSH primário: Negro de Amido/toxicidade
Antioxidantes/metabolismo
Compostos Azo/toxicidade
Naftalenossulfonatos/toxicidade
[Mh] Termos MeSH secundário: Negro de Amido/administração & dosagem
Animais
Compostos Azo/administração & dosagem
Encéfalo/efeitos dos fármacos
Encéfalo/enzimologia
Carpas
Relação Dose-Resposta a Droga
Brânquias/efeitos dos fármacos
Brânquias/enzimologia
Rim/efeitos dos fármacos
Rim/enzimologia
Fígado/efeitos dos fármacos
Fígado/enzimologia
Músculo Esquelético/efeitos dos fármacos
Músculo Esquelético/enzimologia
Naftalenossulfonatos/administração & dosagem
Estresse Oxidativo/efeitos dos fármacos
Poluentes Químicos da Água/administração & dosagem
Poluentes Químicos da Água/toxicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antioxidants); 0 (Azo Compounds); 0 (Naphthalenesulfonates); 0 (Water Pollutants, Chemical); SZT789770M (Amido Black)
[Em] Mês de entrada:1507
[Cu] Atualização por classe:141201
[Lr] Data última revisão:
141201
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141004
[St] Status:MEDLINE


  10 / 112 MEDLINE  
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[PMID]:24135655
[Au] Autor:Heda GD; Kunwar U; Heda RP
[Ad] Endereço:Department of Sciences and Mathematics, Mississippi University for Women, Columbus, MS 39701, USA; Department of Medicine, University of Tennessee Health Sciences Center, Memphis, TN 38163, USA. Electronic address: gheda@as.muw.edu.
[Ti] Título:A modified protein assay from microgram to low nanogram levels in dilute samples.
[So] Source:Anal Biochem;445:67-72, 2014 Jan 15.
[Is] ISSN:1096-0309
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In this article, we present a modified and improved protein assay that was previously described as "amidoschwarz assay" by Schaffner and Weissmann. Our improved protein assay is user-friendly and 30-40 times more sensitive than the earlier method. The assay was developed into three formats (macro-, micro-, and nanoassay) with trichloroacetic acid (TCA) as protein precipitating agent, measuring up to 96 samples. The macro and micro formats of this assay require a single reagent staining with amido black of protein dots bound to nitrocellulose membrane with lowest protein measurements to 1 and 0.1 µg, respectively. On the other hand, the nanoassay, with combination staining of amido black followed by colloidal gold, can extend the detection limit to 2.5 ng of protein. Protein concentrations were determined by densitometry and/or spectrophotometry. This assay is compatible with many ionic and non-ionic detergents. This improved protein assay provides an additional choice to researchers in measuring total protein concentration accurately in dilute biological samples as low as 0.125 µg/ml prior to their biochemical analysis such as in comparative proteomics.
[Mh] Termos MeSH primário: Densitometria
Proteínas/análise
Espectrofotometria
[Mh] Termos MeSH secundário: Negro de Amido/química
Animais
Bovinos
Colódio/química
Detergentes/química
Coloide de Ouro/química
Membranas Artificiais
Proteínas/química
Soroalbumina Bovina/análise
Soroalbumina Bovina/química
Ácido Tricloroacético/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Detergents); 0 (Gold Colloid); 0 (Membranes, Artificial); 0 (Proteins); 27432CM55Q (Serum Albumin, Bovine); 5V2JDO056X (Trichloroacetic Acid); 9004-70-0 (Collodion); SZT789770M (Amido Black)
[Em] Mês de entrada:1407
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:131019
[St] Status:MEDLINE



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