Base de dados : MEDLINE
Pesquisa : D02.172.600 [Categoria DeCS]
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[PMID]:28587857
[Au] Autor:Alonso B; Cruces R; Pérez A; Sánchez-Carrillo C; Guembe M
[Ad] Endereço:Department of Clinical Microbiology and Infectious Diseases, Hospital General Universitario Gregorio Marañón, Madrid, Spain; Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain.
[Ti] Título:Comparison of the XTT and resazurin assays for quantification of the metabolic activity of Staphylococcus aureus biofilm.
[So] Source:J Microbiol Methods;139:135-137, 2017 Aug.
[Is] ISSN:1872-8359
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:We assessed whether resazurin was as efficient as XTT in the measurement of the metabolic activity of 209 clinical Staphylococcus aureus biofilm using an vitro model comparing the percentage of formazan and resorufin. The overall categorical agreement was 61.2% (r=0.024), which means that resazurin can not substitute XTT.
[Mh] Termos MeSH primário: Oxazinas
Staphylococcus aureus/metabolismo
Sais de Tetrazólio/farmacologia
Xantenos
[Mh] Termos MeSH secundário: Biofilmes/crescimento & desenvolvimento
Formazans/análise
Oxazinas/análise
Coloração e Rotulagem
Infecções Estafilocócicas/microbiologia
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Formazans); 0 (Oxazines); 0 (Tetrazolium Salts); 0 (Xanthenes); 117038-70-7 (2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-((phenylamino)carbonyl)-2H-tetrazolium hydroxide); 1FN9YD6968 (resazurin); 635-78-9 (resorufin)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171107
[Lr] Data última revisão:
171107
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170608
[St] Status:MEDLINE


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Ponzoni, Deise
Puricelli, Edela
Texto completo SciELO Brasil
[PMID]:28198971
[Au] Autor:Corsetti A; Bahuschewskyj C; Ponzoni D; Langie R; Santos LA; Camassola M; Nardi NB; Puricelli E
[Ad] Endereço:Universidade Federal do Rio Grande do Sul, Faculdade de Odontologia, Porto Alegre, RS, Brasil.
[Ti] Título:Repair of bone defects using adipose-derived stem cells combined with alpha-tricalcium phosphate and gelatin sponge scaffolds in a rat model.
[So] Source:J Appl Oral Sci;25(1):10-19, 2017 Jan-Feb.
[Is] ISSN:1678-7765
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:Objectives: This study aimed to evaluate the potential of adipose-derived stem cells (ASCs) combined with a modified α-tricalcium phosphate (α-TCP) or gelatin sponge (GS) scaffolds for bone healing in a rat model. Material and Methods: Bone defects were surgically created in the femur of adult SHR rats and filled with the scaffolds, empty or combined with ASCs. The results were analyzed by histology and histomorphometry on days seven, 14, 30, and 60. Results: Significantly increased bone repair was observed on days seven and 60 in animals treated with α-TCP/ASCs, and on day 14 in the group treated with GS/ASCs, when compared with the groups treated with the biomaterials alone. Intense fibroplasia was observed in the group treated with GS alone, on days 14 and 30. Conclusions: Our results showed that the use of ASCs combined with α-TCP or GS scaffolds resulted in increased bone repair. The higher efficacy of the α-TCP scaffold suggests osteoconductive property that results in a biological support to the cells, whereas the GS scaffold functions just as a carrier. These results confirm the potential of ASCs in accelerating bone repair in in vivo experimental rat models. These results suggest a new alternative for treating bone defects.
[Mh] Termos MeSH primário: Tecido Adiposo/citologia
Materiais Biocompatíveis/farmacologia
Regeneração Óssea/efeitos dos fármacos
Fosfatos de Cálcio/farmacologia
Esponja de Gelatina Absorvível/farmacologia
Transplante de Células-Tronco/métodos
Tecidos Suporte
[Mh] Termos MeSH secundário: Animais
Materiais Biocompatíveis/uso terapêutico
Fosfatos de Cálcio/uso terapêutico
Adesão Celular/efeitos dos fármacos
Proliferação Celular/efeitos dos fármacos
Células Cultivadas
Fêmur/patologia
Fêmur/cirurgia
Fibroblastos/efeitos dos fármacos
Formazans
Esponja de Gelatina Absorvível/uso terapêutico
Masculino
Modelos Animais
Osteogênese/efeitos dos fármacos
Ratos Endogâmicos SHR
Reprodutibilidade dos Testes
Sais de Tetrazólio
Fatores de Tempo
Resultado do Tratamento
Cicatrização/efeitos dos fármacos
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biocompatible Materials); 0 (Calcium Phosphates); 0 (Formazans); 0 (Tetrazolium Salts); 0 (alpha-tricalcium phosphate); 23305-68-2 (MTT formazan)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170403
[Lr] Data última revisão:
170403
[Sb] Subgrupo de revista:D; IM
[Da] Data de entrada para processamento:170216
[St] Status:MEDLINE


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[PMID]:28117201
[Au] Autor:Saharan VD; Mahajan SS
[Ad] Endereço:Department of Pharmaceutical Chemistry, C.U. Shah College of Pharmacy, S.N.D.T. Women's University, Sir Vithaldas Vidyavihar, Juhu Tara Road, Santacruz (West), Mumbai 400049, India. Electronic address: vanitasaharan@yahoo.co.in.
[Ti] Título:Development of gallic acid formazans as novel enoyl acyl carrier protein reductase inhibitors for the treatment of tuberculosis.
[So] Source:Bioorg Med Chem Lett;27(4):808-815, 2017 Feb 15.
[Is] ISSN:1464-3405
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The enoyl acyl carrier protein reductase (InhA) of Mycobacterium tuberculosis (MTB) is an attractive target for developing novel antitubercular agents. A series of gallic acid formazans, were computationally designed and docked into the active site of InhA to understand their binding mode and potential to inhibit InhA. Nine compounds from the designed series were identified as potential InhA inhibitors, on the basis of good Glide score. These compounds were synthesized in the laboratory and evaluated for in vitro antitubercular activity against drug-sensitive and multi-drug resistant strains of MTB. Out of nine compounds, three compounds exhibited the most promising MIC of <2µM against the sensitive strain of MTB, H37Rv. The compounds were evaluated against five resistant strains of MTB. Most of the compounds exhibited activity superior to the standard, linezolid, against all these resistant strains. The mechanism of action of these compounds was concluded to be InhA inhibition, through InhA enzyme inhibition study. Insignificant cytotoxicity of these compounds was observed on RAW 264.7 cell line. Inactivity of all these compounds against gram positive and gram negative bacteria indicated their specificity against MTB. The compounds were further analyzed for ADME properties and showed potential as good oral drug candidates. The results clearly identified some novel, selective and specific InhA inhibitors against sensitive and resistant strains of MTB.
[Mh] Termos MeSH primário: Antituberculosos/química
Proteínas de Bactérias/antagonistas & inibidores
Formazans/química
Ácido Gálico/química
Oxirredutases/antagonistas & inibidores
[Mh] Termos MeSH secundário: Animais
Antibacterianos/química
Antibacterianos/farmacologia
Antituberculosos/farmacologia
Antituberculosos/uso terapêutico
Proteínas de Bactérias/metabolismo
Sítios de Ligação
Domínio Catalítico
Formazans/farmacologia
Formazans/uso terapêutico
Bactérias Gram-Negativas/efeitos dos fármacos
Bactérias Gram-Positivas/efeitos dos fármacos
Seres Humanos
Camundongos
Testes de Sensibilidade Microbiana
Simulação de Acoplamento Molecular
Mycobacterium tuberculosis/efeitos dos fármacos
Mycobacterium tuberculosis/enzimologia
Oxirredutases/metabolismo
Células RAW 264.7
Tuberculose/tratamento farmacológico
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Antitubercular Agents); 0 (Bacterial Proteins); 0 (Formazans); 632XD903SP (Gallic Acid); EC 1.- (Oxidoreductases); EC 1.3.1.9 (InhA protein, Mycobacterium)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170704
[Lr] Data última revisão:
170704
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170125
[St] Status:MEDLINE


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[PMID]:27659119
[Au] Autor:Muhammad ZA; Masaret GS; Amin MM; Abdallah MA; Farghaly TA
[Ad] Endereço:National Organization for Drug Control and Research (NODCAR), P.O. Box 29, Cairo, Egypt.
[Ti] Título:Anti-inflammatory, Analgesic and Anti-ulcerogenic Activities of Novel bis-thiadiazoles, bis-thiazoles and bis-formazanes.
[So] Source:Med Chem;13(3):226-238, 2017.
[Is] ISSN:1875-6638
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Indane-1,3-dione, thiazole, bis-thiazole and thiadiazoles rings are very interested moieties in anti-inflammatory and analgesic drugs. OBJECTIVE: The goal of this work is to synthesize new derivatives of bis-thiazoles and bis-1,3,4- thiadiazoles for the investigation of their anti-inflammatory, anti-ulcerogenic and analgesic activities. METHODS: 1,1'-(1,2-phenylene)bis(3-phenylthiourea) (1) reacts with a number of N-aryl arenecarbohydrazonoyl chlorides 2 to give a series of new bis-1,3,4-thiadiazoles 4. Also, reaction of bisthiosemicarbazone of 1,3-indanedione 6 with another type of hydrazonoyl halides namely, N-aryl-2- oxapropanehydrazonoyl chlorides 7 and ethyl-(N-arylhydrazono)chloroacetate 8 in dioxane under reflux in the presence of triethylamine give the respective bis-thiazole derivatives 9 and 10, respectively. The products 9 and 10 can exist in five and seven tautomeric forms for each one. Their actual tautomeric forms were deduced based on electronic absorption data (UV / Vis spectra). Moreover, a series of novel bis-formazans 12 and 13 have been synthesized by reaction of 1,3-dihydrazono-2,3- dihydro-1H-indene (11) with both hydrazonoyl chlorides 7 and 8. RESULTS: The structure of all the novel products was deduced by elemental analysis and spectral data. In addition, the biological activity of the newly synthesized compounds was evaluated and the results obtained indicate their potency as anti-inflammatory, anti-ulcerogenic and analgesic agents. CONCLUSION: In this context, we synthesize new derivatives of bis-thiazoles and bis-1,3,4-thiadiazoles as anti-inflammatory, anti-ulcerogenic and analgesic agents.
[Mh] Termos MeSH primário: Analgésicos/farmacologia
Anti-Inflamatórios não Esteroides/farmacologia
Antiulcerosos/farmacologia
Formazans/farmacologia
Tiazóis/farmacologia
[Mh] Termos MeSH secundário: Analgésicos/síntese química
Analgésicos/química
Animais
Anti-Inflamatórios não Esteroides/síntese química
Anti-Inflamatórios não Esteroides/química
Antiulcerosos/síntese química
Antiulcerosos/química
Relação Dose-Resposta a Droga
Edema/tratamento farmacológico
Formazans/síntese química
Formazans/química
Masculino
Camundongos
Estrutura Molecular
Dor Nociceptiva/tratamento farmacológico
Ratos
Ratos Sprague-Dawley
Úlcera Gástrica/tratamento farmacológico
Relação Estrutura-Atividade
Tiazóis/síntese química
Tiazóis/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Analgesics); 0 (Anti-Inflammatory Agents, Non-Steroidal); 0 (Anti-Ulcer Agents); 0 (Formazans); 0 (Thiazoles)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170829
[Lr] Data última revisão:
170829
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160924
[St] Status:MEDLINE
[do] DOI:10.2174/1573406412666160920091146


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[PMID]:28035114
[Au] Autor:Eskandari EG; Doudi M; Abedi S
[Ad] Endereço:Department of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran.
[Ti] Título:An study of antileishmanial effect of extract.
[So] Source:J Vector Borne Dis;53(4):362-369, 2016 Oct-Dec.
[Is] ISSN:0972-9062
[Cp] País de publicação:India
[La] Idioma:eng
[Ab] Resumo:BACKGROUND & OBJECTIVES: Leishmaniasis is caused by protozoa of Leishmania genus and is considered as a zoonotic disease. It is a major public health problem worldwide, with high endemicity in developing countries like Iran. Various chemical drugs are used for leishmaniasis treatment, but their side-effects and the emergence of drug resistance have led to look for new effective compounds. The aim of this study was to introduce purslane (Portulaca oleracea) as a traditional and medicinal herb which might act as a valuable source for designing new pharmaceutical drug/lead against Leishmania sp. METHODS: This study was conducted in the laboratory of Seddigheh Tahereh Infectious Disease Research Center, Isfahan, Iran during the spring of 2015. The essence from the purslane plant was prepared through water distillation and the alcoholic extract was prepared through maceration method. The essence was dried, and diluted with DMSO (5%). Leishmania major promastigotes were cultured in 25 ΁ 2΀C temperature in the stationary phase of RPMI-1640 medium, enriched with 10% fetal calf serum and penicillin-streptomycin to yield higher quantity. The biological activity of herb essence was evaluated on L. major promastigotes and compared to glucantime reference drug using methylthiazole tetrazolium (MTT) colorometric assay. The optical density absorbance was measured with Eliza reader set, and the IC50 value was calculated at different time intervals. All tests were repeated thrice. Results were analyzed by using Tukey test and t-test. RESULTS: The IC50 values after 48 h, for glucantime against standard parasite promastigotes and clinical strains were equal to 12 and 19 mg/ml, respectively, whereas for purslane herb leaves and stems essence; it was equal to 360 and 680 mg/ml, respectively. Although, the glucantime pharmaceutical drug was more efficient compared to the investigated herb essence, the essense had significant effect on L. major promastigotes with increasing density (p <0.05). The ingredients of the herb leaves and stem essence were-Phytol, squalene, palmitic acid, ethyl- linoleate, ferulic acid, linolenic acid, scopoletin, linoleic acid, rhein, apigenin, and bergapten. INTERPRETATION & CONCLUSION: The study showed that essence of purslane has considerable antileishmanial effects and can stop the growth of parasites in the laboratory compared to glucantime. More experiments are necessary to investigate its effect on Leishmania parasite in animal model.
[Mh] Termos MeSH primário: Antiprotozoários/farmacologia
Leishmania major/efeitos dos fármacos
Extratos Vegetais/farmacologia
Portulaca/química
[Mh] Termos MeSH secundário: Antiprotozoários/isolamento & purificação
Sobrevivência Celular/efeitos dos fármacos
Colorimetria/métodos
Formazans/análise
Concentração Inibidora 50
Irã (Geográfico)
Testes de Sensibilidade Parasitária
Extratos Vegetais/isolamento & purificação
Espectrofotometria
Sais de Tetrazólio/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antiprotozoal Agents); 0 (Formazans); 0 (Plant Extracts); 0 (Tetrazolium Salts); 23305-68-2 (MTT formazan)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170414
[Lr] Data última revisão:
170414
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161231
[St] Status:MEDLINE


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[PMID]:27701717
[Au] Autor:Ghasemzadeh P; Rezayat SM; Adeli S; Rahbar-Roshandel N
[Ad] Endereço:Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran.
[Ti] Título:Protective Effect of 25Mg-Porphyrin-Fullerene Nanoparticles on Oxygen-Glucose Deprivation/Reperfusion Injury in PC12 Cells.
[So] Source:Acta Med Iran;54(8):478-484, 2016 Aug.
[Is] ISSN:1735-9694
[Cp] País de publicação:Iran
[La] Idioma:eng
[Ab] Resumo:We investigated the effects of 25Mg-Porphyrin-Fullerene nanoparticles, (25MgPMC16) smart ferroporphyrin nanoparticles, on PC12 cells exposed to oxygen-glucose deprivation/reperfusion. In order to explore its effect on cells under oxygen-glucose deprivation conditions, the cultures were pretreated with 25MgPMC16 24 hours prior to oxygen-glucose deprivation/reperfusion. To initiate the oxygen-glucose deprivation/reperfusion, the cell culture medium was replaced with a glucose-free medium and the cells were transferred to a humidified incubation chamber in a mixture of 95% N2 and 5% CO2 at 37° C for 30, 60 and 120 min. Cell viability was assessed by MTT assay. Exposure of PC12 cells to 30, 60 and 120 min oxygen-glucose deprivation significantly decreased the cell viability. Pretreatment of the cultures with 25MgPMC16 significantly increased cell viability in a concentration-dependent manner. Pretreatment, the cultures with MK-801 (10 µM), a non-competitive NMDA antagonist, has attenuated the cell death after 30 min oxygen-glucose deprivation. We concluded that 25MgPMC16 could protect PC12 cells against oxygen-glucose deprivation/reperfusion-induced cell injury in a concentration-dependent manner. That could be due to the effect of 25MgPMC16 on ATP synthesis and the antioxidant effects of its components.
[Mh] Termos MeSH primário: Sobrevivência Celular/efeitos dos fármacos
Relação Dose-Resposta a Droga
Fulerenos/administração & dosagem
Fulerenos/farmacologia
Glucose/deficiência
Oxigênio/sangue
Células PC12/efeitos dos fármacos
Células PC12/metabolismo
Porfirinas/administração & dosagem
Porfirinas/farmacologia
Traumatismo por Reperfusão/tratamento farmacológico
[Mh] Termos MeSH secundário: Animais
Técnicas de Cultura de Células
Morte Celular
Formazans/metabolismo
Glucose/metabolismo
Ratos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Formazans); 0 (Fullerenes); 0 (Porphyrins); IY9XDZ35W2 (Glucose); S88TT14065 (Oxygen)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170116
[Lr] Data última revisão:
170116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161005
[St] Status:MEDLINE


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[PMID]:27616076
[Au] Autor:Flores ME; Garcés-Jerez P; Fernández D; Aros-Perez G; González-Cabrera D; Álvarez E; Cañas I; Oyarzun-Ampuero F; Moreno-Villoslada I
[Ad] Endereço:Instituto de Ciencias Químicas, Facultad de Ciencias, Universidad Austral de Chile, Las Encinas 220, Valdivia, 5110033, Chile.
[Ti] Título:Facile Formation of Redox-Active Totally Organic Nanoparticles in Water by In Situ Reduction of Organic Precursors Stabilized through Aromatic-Aromatic Interactions by Aromatic Polyelectrolytes.
[So] Source:Macromol Rapid Commun;37(21):1729-1734, 2016 Nov.
[Is] ISSN:1521-3927
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:The formation of redox-active, totally organic nanoparticles in water is achieved following a strategy similar to that used to form metal nanoparticles. It is based on two fundamental concepts: i) complexation through aromatic-aromatic interactions of a water-soluble precursor aromatic molecule with polyelectrolytes bearing complementary charged aromatic rings, and ii) reduction of the precursor molecule to achieve stabilized nanoparticles. Thus, formazan nanoparticles are synthesized by reduction of a tetrazolium salt with ascorbic acid using polyelectrolytes bearing benzene sulfonate residues of high linear aromatic density, but cannot be formed in the presence of nonaromatic polyelectrolytes. The red colored nanoparticles are efficiently encapsulated in calcium alginate beads, showing macroscopic homogeneity. Bleaching kinetics with chlorine show linear rates on the order of tenths of milli-meters per minute. A linear behavior of the dependence of the rate of bleaching on the chlorine concentration is found, showing the potential of the nanoparticles for chlorine sensing.
[Mh] Termos MeSH primário: Eletrólitos/química
Formazans/química
Hidrocarbonetos Aromáticos/química
Nanopartículas/química
Polímeros/química
Sais de Tetrazólio/química
Água/química
[Mh] Termos MeSH secundário: Oxirredução
Tamanho da Partícula
Propriedades de Superfície
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (1,3,5-triphenylformazan); 0 (Electrolytes); 0 (Formazans); 0 (Hydrocarbons, Aromatic); 0 (Polymers); 0 (Tetrazolium Salts); 059QF0KO0R (Water); 7OL20RET2I (triphenyltetrazolium)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170411
[Lr] Data última revisão:
170411
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160913
[St] Status:MEDLINE
[do] DOI:10.1002/marc.201600339


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[PMID]:27565090
[Au] Autor:Sun M; Zhou Z; Dong J; Zhang J; Xia Y; Shu R
[Ad] Endereço:Department of Periodontology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai 200011, China.
[Ti] Título:Antibacterial and antibiofilm activities of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) against periodontopathic bacteria.
[So] Source:Microb Pathog;99:196-203, 2016 Oct.
[Is] ISSN:1096-1208
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) are two major omega-3 polyunsaturated fatty acids (n-3 PUFAs) with antimicrobial properties. In this study, we evaluated the potential antibacterial and antibiofilm activities of DHA and EPA against two periodontal pathogens, Porphyromonas gingivalis (P. gingivalis) and Fusobacterium nucleatum (F. nucleatum). MTT assay showed that DHA and EPA still exhibited no cytotoxicity to human oral tissue cells when the concentration came to 100 µM and 200 µM, respectively. Against P. gingivalis, DHA and EPA showed the same minimum inhibitory concentration (MIC) of 12.5 µM, and a respective minimum bactericidal concentration (MBC) of 12.5 µM and 25 µM. However, the MIC and MBC values of DHA or EPA against F. nucleatum were both greater than 100 µM. For early-stage bacteria, DHA or EPA displayed complete inhibition on the planktonic growth and biofilm formation of P. gingivalis from the lowest concentration of 12.5 µM. And the planktonic growth of F. nucleatum was slightly but not completely inhibited by DHA or EPA even at the concentration of 100 µM, however, the biofilm formation of F. nucleatum at 24 h was significantly restrained by 100 µM EPA. For exponential-phase bacteria, 100 µM DHA or EPA completely killed P. gingivalis and significantly decreased the viable counts of F. nucleatum. Meanwhile, the morphology of P. gingivalis was apparently damaged, and the virulence factor gene expression of P. gingivalis and F. nucleatum was strongly downregulated. Besides, the viability and the thickness of mature P. gingivalis biofilm, together with the viability of mature F. nucleatum biofilm were both significantly decreased in the presence of 100 µM DHA or EPA. In conclusion, DHA and EPA possessed antibacterial activities against planktonic and biofilm forms of periodontal pathogens, which suggested that DHA and EPA might be potentially supplementary therapeutic agents for prevention and treatment of periodontal diseases.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Biofilmes/efeitos dos fármacos
Ácidos Docosa-Hexaenoicos/farmacologia
Ácido Eicosapentaenoico/farmacologia
Fusobacterium nucleatum/efeitos dos fármacos
Porphyromonas gingivalis/efeitos dos fármacos
[Mh] Termos MeSH secundário: Antibacterianos/toxicidade
Biofilmes/crescimento & desenvolvimento
Sobrevivência Celular/efeitos dos fármacos
Células Cultivadas
Ácidos Docosa-Hexaenoicos/toxicidade
Ácido Eicosapentaenoico/toxicidade
Células Epiteliais/efeitos dos fármacos
Células Epiteliais/fisiologia
Formazans/análise
Fusobacterium nucleatum/citologia
Fusobacterium nucleatum/genética
Fusobacterium nucleatum/fisiologia
Expressão Gênica/efeitos dos fármacos
Perfilação da Expressão Gênica
Seres Humanos
Testes de Sensibilidade Microbiana
Viabilidade Microbiana/efeitos dos fármacos
Porphyromonas gingivalis/citologia
Porphyromonas gingivalis/genética
Porphyromonas gingivalis/fisiologia
Coloração e Rotulagem
Sais de Tetrazólio/análise
Fatores de Virulência/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Formazans); 0 (Tetrazolium Salts); 0 (Virulence Factors); 23305-68-2 (MTT formazan); 25167-62-8 (Docosahexaenoic Acids); AAN7QOV9EA (Eicosapentaenoic Acid)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170327
[Lr] Data última revisão:
170327
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160828
[St] Status:MEDLINE


  9 / 1115 MEDLINE  
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[PMID]:27556203
[Au] Autor:Fernandes AP; Junqueira Mde A; Marques NC; Machado MA; Santos CF; Oliveira TM; Sakai VT
[Ad] Endereço:- Universidade de São Paulo, Faculdade de Odontologia de Bauru, Departamento de Odontopediatria, Ortodontia e Saúde Coletiva, Bauru, SP, Brasil.
[Ti] Título:Effects of low-level laser therapy on stem cells from human exfoliated deciduous teeth.
[So] Source:J Appl Oral Sci;24(4):332-7, 2016 Jul-Aug.
[Is] ISSN:1678-7765
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: This study aimed to evaluate the influence of different laser therapy energy densities on SHED viability and proliferation. MATERIAL AND METHODS: SHED were irradiated according to the groups: I (1.2 J/cm2 - 0.5 mW - 10 s), II (2.5 J/cm2 - 10 mW - 10 s), III (3.7 J/cm2 - 15 mW - 10 s), IV (5.0 J/cm2 - 20 mW - 10 s), V (6.2 J/cm2 - 25 mW - 10 s), and VI (not irradiated - control group). Cell viability was assessed 6 and 24 h after irradiation measuring the mitochondrial activity and using the Crystal Violet assay. Cell proliferation was assessed after 24, 48, and 72 h of irradiation by SRB assay. RESULTS: MTT assay demonstrated differences from 6 to 24 hours after irradiation. After 24 h, groups I and IV showed higher absorbance values than those of control group. Crystal Violet assay showed statistically differences in the absorbance rate from 6 to 24 h after irradiation for groups III and VI. At 24 h after irradiation, Group III absorbance rate was greater than that of groups I, II, and IV. Group VI absorbance rate was greater than that of groups I and IV. SRB assay showed that the group I had higher rates than those of groups II, III, V, and VI, at 24 h after irradiation. After 48 h, group I exhibited the greatest cell proliferation rate followed by groups III, V, and VI. After 72 h, group III exhibited the lowest cell proliferation rate than those of groups II, IV, and V. CONCLUSIONS: The Low-Level Laser Therapy energy densities used in this study did not cause loss of cell viability and stimulated SHED proliferation within the parameters described in this study.
[Mh] Termos MeSH primário: Terapia com Luz de Baixa Intensidade/métodos
Células-Tronco/efeitos da radiação
Esfoliação de Dente
Dente Decíduo/citologia
Dente Decíduo/efeitos da radiação
[Mh] Termos MeSH secundário: Análise de Variância
Proliferação Celular/efeitos da radiação
Sobrevivência Celular/efeitos da radiação
Células Cultivadas
Formazans
Seres Humanos
Dose de Radiação
Reprodutibilidade dos Testes
Rodaminas
Sais de Tetrazólio
Fatores de Tempo
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Formazans); 0 (Rhodamines); 0 (Tetrazolium Salts); 23305-68-2 (MTT formazan); 2609-88-3 (lissamine rhodamine B)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:D; IM
[Da] Data de entrada para processamento:160825
[St] Status:MEDLINE


  10 / 1115 MEDLINE  
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[PMID]:27463630
[Au] Autor:Covre JL; Cristovam PC; Loureiro RR; Hazarbassanov RM; Campos M; Sato ÉH; Gomes JÁ
[Ad] Endereço:Ocular Surface Advanced Center (CASO), Department of Ophthalmology and Visual Sciences, Escola Paulista de Medicina (EPM), Universidade Federal de São Paulo (UNIFESP), São Paulo, SP, Brazil.
[Ti] Título:The effects of riboflavin and ultraviolet light on keratocytes cultured in vitro.
[So] Source:Arq Bras Oftalmol;79(3):180-5, 2016 May-Jun.
[Is] ISSN:1678-2925
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:PURPOSE: To culture quiescent human keratocytes and evaluate the effects of ultraviolet light and riboflavin on human corneal keratocytes in vitro. METHODS: Keratocytes were obtained from remaining corneoscleral ring donor corneas previously used in corneal transplant surgeries and cultured in DMEM/F12 with 2% FBS until confluence. Characterization of cultured cells was performed by immunofluorescence analysis for anti-cytokeratin-3, anti-Thy-1, anti-α-smooth muscle actin, and anti-lumican. Immunofluorescence was performed before and after treatment of cultured cells with either ultraviolet light or riboflavin. Corneal stromal cells were covered with collagen (200 µL or 500 µL) and 0.1% riboflavin, and then exposed to ultraviolet light at 370 nm for 30 minutes. After 24 hours, cytotoxicity was determined using MTT colorimetric assays, whereas cell viability was assessed using Hoechst 33342 and propidium iodide. RESULTS: Cell cultures achieved confluence in approximately 20 days. Expression of the lumican was high, whereas no expression of CK3, Thy-1, and α-SMA was observed. After crosslinking, MTT colorimetric assays demonstrated a low toxicity rate, whereas Hoechst 33342/propidium iodide staining demonstrated a low rate of apoptosis and necrosis, respectively, in all collagen-treatment groups. CONCLUSION: Keratocytes can be successfully cultured in vitro and characterized by immunofluorescence using lumican. MTT colorimetric assays, and Hoechst 33342, and propidium iodide staining demonstrated a higher rate of cell death in cells cultured without collagen, indicating collagen protects keratocytes from the cytotoxic effects of ultraviolet light.
[Mh] Termos MeSH primário: Ceratócitos da Córnea/efeitos dos fármacos
Ceratócitos da Córnea/efeitos da radiação
Fármacos Fotossensibilizantes/farmacologia
Riboflavina/farmacologia
Raios Ultravioleta
[Mh] Termos MeSH secundário: Análise de Variância
Apoptose/efeitos dos fármacos
Apoptose/efeitos da radiação
Sobrevivência Celular/efeitos dos fármacos
Sobrevivência Celular/efeitos da radiação
Células Cultivadas
Colágeno/farmacologia
Substância Própria/citologia
Reagentes para Ligações Cruzadas/farmacologia
Fibroblastos/efeitos dos fármacos
Fibroblastos/efeitos da radiação
Imunofluorescência
Formazans
Seres Humanos
Necrose
Estatísticas não Paramétricas
Sais de Tetrazólio
Fatores de Tempo
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cross-Linking Reagents); 0 (Formazans); 0 (Photosensitizing Agents); 0 (Tetrazolium Salts); 23305-68-2 (MTT formazan); 9007-34-5 (Collagen); TLM2976OFR (Riboflavin)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160728
[St] Status:MEDLINE



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