Base de dados : MEDLINE
Pesquisa : D02.241.081 [Categoria DeCS]
Referências encontradas : 144 [refinar]
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[PMID]:29216582
[Au] Autor:Fiori J; Amadesi E; Fanelli F; Tropeano CV; Rugolo M; Gotti R
[Ad] Endereço:Department of Pharmacy and Biotechnology, University of Bologna, Via Belmeloro 6, 40126, Bologna, Italy. Electronic address: jessica.fiori@unibo.it.
[Ti] Título:Cellular and mitochondrial determination of low molecular mass organic acids by LC-MS/MS.
[So] Source:J Pharm Biomed Anal;150:33-38, 2018 Feb 20.
[Is] ISSN:1873-264X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A selective and sensitive method for the determination of low molecular mass organic acids (LMMOAs) in cell and mitochondrial extracts is presented. The analytical method consists in the separation by reversed phase liquid chromatography and detection with tandem mass spectrometry (LC-MS/MS) of the LMMOAs like malic, succinic, formic and citric acids. These acids are among the cellular intermediates of the tricarboxylic acid cycle (TCA), thus their quantitation can provide essential information about the catabolic and anabolic processes occurring in cells under physiological and pathological conditions. The analytical method was fully validated in terms of linearity, detection and quantification limits, recovery and precision. Detection limits (LOD) for malic, succinic and fumaric acids were in the range of 1-10nM, while 20nM was obtained for citric acid. Analytical recovery in cell and mitochondrial extracts was found between 88 and 105% (CV% ≤7.1) and matrix effect was estimated to be less than 108%. The LC-MS/MS method applied to the quantification of TCA cycle metabolites revealed a different distribution of the four acids in cells and mitochondria, and it could be used to monitoring metabolic alterations associated with TCA cycle and oxidative phosphorylation dysfunctions.
[Mh] Termos MeSH primário: Ácidos Acíclicos/análise
Cromatografia de Fase Reversa/métodos
Espectrometria de Massas em Tandem/métodos
[Mh] Termos MeSH secundário: Ácidos Acíclicos/metabolismo
Ciclo do Ácido Cítrico
Seres Humanos
Limite de Detecção
Mitocôndrias/metabolismo
Peso Molecular
Compostos Orgânicos/análise
Compostos Orgânicos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; VALIDATION STUDIES
[Nm] Nome de substância:
0 (Acids, Acyclic); 0 (Organic Chemicals)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180130
[Lr] Data última revisão:
180130
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171208
[St] Status:MEDLINE


  2 / 144 MEDLINE  
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[PMID]:28624740
[Au] Autor:Dinh QT; Li Z; Tran TAT; Wang D; Liang D
[Ad] Endereço:College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi 712100, China.
[Ti] Título:Role of organic acids on the bioavailability of selenium in soil: A review.
[So] Source:Chemosphere;184:618-635, 2017 Oct.
[Is] ISSN:1879-1298
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Organic Acids (OAs) are important components in the rhizosphere soil and influence Se bioavailability in soil. OAs have a bidirectional contrasting effect on Se bioavailability. Understanding the interaction of OAs with Se is essential to assessing Se bioavailability in soil and clarifying the role of OAs in controlling the behavior and fate of Se in soil. This review examines the mechanisms for the (im)mobilization of Se by OAs and discusses the practical implications of these mechanisms in relation to sequestration and bioavailability of Se in soil.
[Mh] Termos MeSH primário: Ácidos Acíclicos/farmacocinética
Compostos Orgânicos/farmacologia
Selênio/farmacocinética
Solo/química
[Mh] Termos MeSH secundário: Disponibilidade Biológica
Rizosfera
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Acids, Acyclic); 0 (Organic Chemicals); 0 (Soil); H6241UJ22B (Selenium)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170619
[St] Status:MEDLINE


  3 / 144 MEDLINE  
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[PMID]:28214934
[Au] Autor:Zhou C; Huang M; Yu J; Li Y; Liu A
[Ad] Endereço:College of Forestry, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
[Ti] Título:The effect of low-molecular-weight organic acids on copper toxicity in E. fetida in an acute exposure system.
[So] Source:Environ Sci Pollut Res Int;24(9):8805-8813, 2017 Mar.
[Is] ISSN:1614-7499
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:In the present study, the effects of low-molecular-weight organic acids (OAs) on the toxicity of copper (Cu) to the earthworm Eisenia fetida (E. fetida) were investigated in a simulated soil solution. We exposed E. fetida to soil solution containing Cu and a variety of OAs (acetic acid, oxalic acid, citric acid, and EDTA). We found that the addition of OAs reduced the toxicity of Cu to E. fetida, where the reduction was strongest in EDTA and weakest in acetic acid. These compounds decreased the mortality rate of E. fetida that were exposed to Cu and reduced levels of antioxidant enzymes and malondialdehyde to unexposed control levels. E. fetida were exposed to Cu with OAs had reduced Cu , which were likely caused by Cu forming complexes with the OAs, reducing the availability of Cu. The presence of OAs also reduced Cu-induced damage on earthworm cellular ultrastructures and changed the subcellular distribution of Cu. These results demonstrated that OAs could reduce the toxicity, as well as the bioavailability, of heavy metals in soil solutions where both OAs and heavy metals often coexist.
[Mh] Termos MeSH primário: Ácidos Acíclicos/farmacologia
Cobre/toxicidade
Oligoquetos/efeitos dos fármacos
Poluentes do Solo/toxicidade
Solo/química
[Mh] Termos MeSH secundário: Ácidos Acíclicos/química
Animais
Disponibilidade Biológica
Cobre/metabolismo
Peso Molecular
Oligoquetos/ultraestrutura
Poluentes do Solo/metabolismo
Soluções
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acids, Acyclic); 0 (Soil); 0 (Soil Pollutants); 0 (Solutions); 789U1901C5 (Copper)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170220
[St] Status:MEDLINE
[do] DOI:10.1007/s11356-017-8594-4


  4 / 144 MEDLINE  
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[PMID]:27988451
[Au] Autor:Onireti OO; Lin C; Qin J
[Ad] Endereço:School of Environment and Life Science, University of Salford, Greater Manchester, M5 4WT, United Kingdom.
[Ti] Título:Combined effects of low-molecular-weight organic acids on mobilization of arsenic and lead from multi-contaminated soils.
[So] Source:Chemosphere;170:161-168, 2017 Mar.
[Is] ISSN:1879-1298
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A batch experiment was conducted to examine the combined effects of three common low-molecular-weight organic acids (LMWOAs) on the mobilization of arsenic and lead in different types of multi-contaminated soils. The capacity of individual LMWOAs (at a same molar concentration) to mobilize soil-borne As and Pb varied significantly. The combination of the organic acids did not make a marked "additive" effect on the mobilization of the investigated three elements. An "antagonistic" effect on element mobilization was clear in the treatments involving oxalic acid for some soils. The acid strength of a LMWOA did not play an important role in controlling the mobilization of elements. While the mobilization of As and Pb was closely associated with the dissolution of soil-borne Fe, soil properties such as original soil pH, organic matter contents and the total amount of the element relative to the total Fe markedly complicated the mobility of that element. Aging led to continual consumption of proton introduced from addition of LMWOAs and consequently caused dramatic changes in solution-borne Fe, which in turn resulted in change in As and Pb in the soil solution though different elements behaved differently.
[Mh] Termos MeSH primário: Ácidos Acíclicos/análise
Arsênico/análise
Chumbo/análise
Poluentes do Solo/análise
Solo/química
[Mh] Termos MeSH secundário: Ácidos Acíclicos/química
Arsênico/química
Ácido Cítrico/análise
Ácido Cítrico/química
Concentração de Íons de Hidrogênio
Chumbo/química
Modelos Teóricos
Peso Molecular
Ácido Oxálico/análise
Ácido Oxálico/química
Poluentes do Solo/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acids, Acyclic); 0 (Soil); 0 (Soil Pollutants); 2968PHW8QP (Citric Acid); 2P299V784P (Lead); 9E7R5L6H31 (Oxalic Acid); N712M78A8G (Arsenic)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161219
[St] Status:MEDLINE


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[PMID]:27605122
[Au] Autor:Liu HL; Wang YJ; Xuan L; Dang F; Zhou DM
[Ad] Endereço:Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, People's Republic of China.
[Ti] Título:Effects of low-molecular-weight organic acids on the acute lethality, accumulation, and enzyme activity of cadmium in Eisenia fetida in a simulated soil solution.
[So] Source:Environ Toxicol Chem;36(4):1005-1011, 2017 Apr.
[Is] ISSN:1552-8618
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In the present study, the effects of low-molecular-weight organic acids (LMWOAs) on the toxicity of cadmium (Cd) to Eisenia fetida were investigated in a simulated soil solution. The LMWOAs protected E. fetida from Cd toxicity, as indicated by the increased median lethal concentration (LC50) values and the increased activity of superoxide dismutase. In addition, Cd concentrations in E. fetida decreased dramatically in the presence of LMWOAs. These results were likely because of the complexation between Cd and LMWOAs, which decreased the bioavailability and consequential toxicity of Cd to E. fetida. Notably, LMWOAs reduced Cd toxicity in decreasing order (ethylenediamine tetraacetic acid [EDTA] > citric acid > oxalic acid > malic acid > acetic acid), which was consistent with the decreasing complexation constants between LMWOAs and Cd. These results advance our understanding of the interactions between Cd and LMWOAs in soil. Environ Toxicol Chem 2017;36:1005-1011. © 2016 SETAC.
[Mh] Termos MeSH primário: Ácidos Acíclicos/farmacologia
Cádmio/toxicidade
Oligoquetos/efeitos dos fármacos
Poluentes do Solo/toxicidade
Solo/química
[Mh] Termos MeSH secundário: Animais
Disponibilidade Biológica
Cádmio/análise
Cádmio/metabolismo
Ácido Cítrico/farmacologia
Malatos/farmacologia
Peso Molecular
Oligoquetos/enzimologia
Oligoquetos/metabolismo
Ácido Oxálico/farmacologia
Poluentes do Solo/análise
Poluentes do Solo/metabolismo
Soluções
Testes de Toxicidade Aguda
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acids, Acyclic); 0 (Malates); 0 (Soil); 0 (Soil Pollutants); 0 (Solutions); 00BH33GNGH (Cadmium); 2968PHW8QP (Citric Acid); 817L1N4CKP (malic acid); 9E7R5L6H31 (Oxalic Acid)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160909
[St] Status:MEDLINE
[do] DOI:10.1002/etc.3618


  6 / 144 MEDLINE  
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[PMID]:27543836
[Au] Autor:Bangma JT; Bowden JA; Brunell AM; Christie I; Finnell B; Guillette MP; Jones M; Lowers RH; Rainwater TR; Reiner JL; Wilkinson PM; Guillette LJ
[Ad] Endereço:Department of Obstetrics and Gynecology, Medical University of South Carolina, Charleston, South Carolina, USA.
[Ti] Título:Perfluorinated alkyl acids in plasma of American alligators (Alligator mississippiensis) from Florida and South Carolina.
[So] Source:Environ Toxicol Chem;36(4):917-925, 2017 Apr.
[Is] ISSN:1552-8618
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The present study aimed to quantitate 15 perfluoroalkyl acids (PFAAs) in 125 adult American alligators at 12 sites across the southeastern United States. Of those 15 PFAAs, 9 were detected in 65% to 100% of samples: perfluorooctanoic acid, perfluorononanoic acid, perfluorodecanoic acid (PFDA), perfluoroundecanoic acid (PFUnA), perfluorododecanoic acid, perfluorotridecanoic acid (PFTriA), perfluorotetradecanoic acid, perfluorohexanesulfonic acid (PFHxS), and perfluorooctane sulfonate (PFOS). Males (across all sites) showed significantly higher concentrations of 4 PFAAs: PFOS (p = 0.01), PFDA (p = 0.0003), PFUnA (p = 0.021), and PFTriA (p = 0.021). Concentrations of PFOS, PFHxS, and PFDA in plasma were significantly different among the sites in each sex. Alligators at both Merritt Island National Wildlife Refuge (FL, USA) and Kiawah Nature Conservancy (SC, USA) exhibited some of the highest PFOS concentrations (medians of 99.5 ng/g and 55.8 ng/g, respectively) in plasma measured to date in a crocodilian species. A number of positive correlations between PFAAs and snout-vent length were observed in both sexes, suggesting that PFAA body burdens increase with increasing size. In addition, several significant correlations among PFAAs in alligator plasma may suggest conserved sources of PFAAs at each site throughout the greater study area. The present study is the first to report PFAAs in American alligators, to reveal potential PFAA hot spots in Florida and South Carolina, and to provide a contaminant of concern when assessing anthropogenic impacts on ecosystem health. Environ Toxicol Chem 2017;36:917-925. Published 2016 Wiley Periodicals Inc. on behalf of SETAC. This article is a US government work and, as such, is in the public domain in the United States of America.
[Mh] Termos MeSH primário: Ácidos Acíclicos/sangue
Jacarés e Crocodilos/sangue
Ecossistema
Monitoramento Ambiental/métodos
Fluorcarbonetos/sangue
[Mh] Termos MeSH secundário: Animais
Feminino
Florida
Ilhas
Masculino
South Carolina
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acids, Acyclic); 0 (Fluorocarbons)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170714
[Lr] Data última revisão:
170714
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160821
[St] Status:MEDLINE
[do] DOI:10.1002/etc.3600


  7 / 144 MEDLINE  
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[PMID]:27197623
[Au] Autor:Weber N; Zupanc V; Jakopic J; Veberic R; Mikulic-Petkovsek M; Stampar F
[Ad] Endereço:Agronomy Department, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, SI-1000, Ljubljana, Slovenia.
[Ti] Título:Influence of deficit irrigation on strawberry (Fragaria × ananassa Duch.) fruit quality.
[So] Source:J Sci Food Agric;97(3):849-857, 2017 Feb.
[Is] ISSN:1097-0010
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Three different irrigation regimes - upper limit of field capacity (UFC), -12 kPa); lower limit of field capacity (LFC), -33 kPa; and deficit irrigation (DI), -70 kPa) were established on silty-loam soil and monitored with tensiometers. Yield and fruit quality of 'Flamenco' and 'Eva's Delight' ever-bearing strawberry cultivars were monitored. The aim of the study was to evaluate the effect of different irrigation regimes on the content of sugars, organic acids and phenolic compounds using high-performance liquid chromatography-mass spectrometry HPLC/HPLC-MS. RESULTS: Deficit irrigation significantly increased the content of sugars (from 1.1- to 1.3 fold), organic acids (from 1.1- to 1.3-fold), their ratio (from 1.1- to 1.2-fold) and the content of most identified phenolics in cv. 'Flamenco'. Conversely, higher amounts of total sugars and organic acids (1.7- to 1.8-fold) were detected in 'Eva's Delight' strawberries at UFC and LFC irrigation. Deficit irrigation generally decreased strawberry yield of cv. 'Eva's Delight'. CONCLUSION: The results suggest superior fruit quality and taste of strawberries grown under minor deficit irrigation for cv. 'Flamenco'. © 2016 Society of Chemical Industry.
[Mh] Termos MeSH primário: Irrigação Agrícola
Conservação dos Recursos Naturais
Produção Agrícola
Produtos Agrícolas/química
Qualidade dos Alimentos
Fragaria/química
Frutas/química
[Mh] Termos MeSH secundário: Ácidos Acíclicos/análise
Ácidos Acíclicos/metabolismo
Antioxidantes/análise
Antioxidantes/metabolismo
Cinamatos/análise
Cinamatos/metabolismo
Produtos Agrícolas/crescimento & desenvolvimento
Produtos Agrícolas/metabolismo
Cruzamentos Genéticos
Carboidratos da Dieta/análise
Flavonoides/análise
Flavonoides/biossíntese
Fragaria/crescimento & desenvolvimento
Fragaria/metabolismo
Frutas/crescimento & desenvolvimento
Frutas/metabolismo
Seres Humanos
Hidroxiácidos/análise
Hidroxiácidos/metabolismo
Fenômenos Mecânicos
Valor Nutritivo
Fenóis/análise
Fenóis/metabolismo
Melhoramento Vegetal
Sensação
Eslovênia
Especificidade da Espécie
Paladar
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acids, Acyclic); 0 (Antioxidants); 0 (Cinnamates); 0 (Dietary Carbohydrates); 0 (Flavonoids); 0 (Hydroxy Acids); 0 (Phenols)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170619
[Lr] Data última revisão:
170619
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160521
[St] Status:MEDLINE
[do] DOI:10.1002/jsfa.7806


  8 / 144 MEDLINE  
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[PMID]:27443952
[Au] Autor:Boriová K; Urík M; Bujdos M; Pifková I; Matús P
[Ad] Endereço:Faculty of Natural Sciences, Comenius University, Mlynská dolina, Ilkovicova 6, Bratislava 842 15, Slovakia.
[Ti] Título:Chemical mimicking of bio-assisted aluminium extraction by Aspergillus niger's exometabolites.
[So] Source:Environ Pollut;218:281-288, 2016 Nov.
[Is] ISSN:1873-6424
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Presence of microorganisms in soils strongly affects mobility of metals. This fact is often excluded when mobile metal fraction in soil is studied using extraction procedures. Thus, the first objective of this paper was to evaluate strain Aspergillus niger's exometabolites contribution on aluminium mobilization. Fungal exudates collected in various time intervals during cultivation were analyzed and used for two-step bio-assisted extraction of alumina and gibbsite. Oxalic, citric and gluconic acids were identified in collected culture media with concentrations up to 68.4, 2.0 and 16.5 mmol L , respectively. These exometabolites proved to be the most efficient agents in mobile aluminium fraction extraction with aluminium extraction efficiency reaching almost 2.2%. However, fungal cultivation is time demanding process. Therefore, the second objective was to simplify acquisition of equally efficient extracting agent by chemically mimicking composition of main organic acid components of fungal exudates. This was successfully achieved with organic acids mixture prepared according to medium composition collected on the 12th day of Aspergillus niger cultivation. This mixture extracted similar amounts of aluminium from alumina compared to culture medium. The aluminium extraction efficiency from gibbsite by organic acids mixture was lesser than 0.09% which is most likely because of more rigid mineral structure of gibbsite compared to alumina. The prepared organic acid mixture was then successfully applied for aluminium extraction from soil samples and compared to standard single step extraction techniques. This showed there is at least 2.9 times higher content of mobile aluminium fraction in soils than it was previously considered, if contribution of microbial metabolites is considered in extraction procedures. Thus, our contribution highlights the significance of fungal metabolites in aluminium extraction from environmental samples, but it also simplifies the extraction procedure inspired by bio-assisted extraction of aluminium by common soil fungus A. niger.
[Mh] Termos MeSH primário: Ácidos Acíclicos/metabolismo
Hidróxido de Alumínio/metabolismo
Óxido de Alumínio/metabolismo
Aspergillus niger/metabolismo
[Mh] Termos MeSH secundário: Alumínio/metabolismo
Ácido Cítrico
Minerais/metabolismo
Solo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acids, Acyclic); 0 (Minerals); 0 (Soil); 2968PHW8QP (Citric Acid); 5QB0T2IUN0 (Aluminum Hydroxide); CPD4NFA903 (Aluminum); LMI26O6933 (Aluminum Oxide)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160723
[St] Status:MEDLINE


  9 / 144 MEDLINE  
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[PMID]:27230401
[Au] Autor:Dams RI; Guilherme AA; Vale MS; Nunes VF; Leitão RC; Santaella ST
[Ad] Endereço:a Brazilian Agricultural Research Corporation , Embrapa Tropical Agroindustry , Fortaleza , Brazil.
[Ti] Título:Fermentation of residual glycerol by Clostridium acetobutylicum ATCC 824 in pure and mixed cultures.
[So] Source:Environ Technol;37(23):2984-92, 2016 Dec.
[Is] ISSN:0959-3330
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The aim of this research was to estimate the production of hydrogen, organic acids and alcohols by the strain of Clostridium acetobutylicum ATCC 824 using residual glycerol as a carbon source. The experiments were carried out in pure and mixed cultures in batch experiments. Three different sources of inocula for mixed culture were used. Ruminal liquid from goats and sludge collected from two upflow anaerobic sludge blanket reactors treating municipal wastewater and brewery effluent were tested for hydrogen, organic acids and alcohols production with or without C. acetobutylicum ATCC 824. The main detected end-products from the glycerol fermentation were hydrogen, organic acids (acetic, propionic, butyric and caproic) and alcohol (ethanol and 1,3-propanediol - 1,3PD). High hydrogen (0.44 mol H2/mol glycerol consumed) and 1,3PD (0.32 mol 1,3PD/mol glycerol consumed) yields were obtained when the strain C. acetobutylicum ATCC 824 was bioaugmented into the sludge from municipal wastewater using 5 g/L of glycerol. Significant concentrations of n-caproic acid were detected in the ruminal liquid when amended with C. acetobutylicum ATCC 824. The results suggest that glycerol can be used for the generation of H2, 1,3PD and n-caproic acid using C. acetobutylicum ATCC 824 as agent in pure or mixed cultures.
[Mh] Termos MeSH primário: Clostridium acetobutylicum/metabolismo
Glicerol/metabolismo
[Mh] Termos MeSH secundário: Ácidos Acíclicos/metabolismo
Animais
Cerveja
Etanol/metabolismo
Fermentação
Conteúdo Gastrointestinal
Cabras
Hidrogênio/metabolismo
Resíduos Industriais
Propilenoglicóis/metabolismo
Rúmen
Esgotos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acids, Acyclic); 0 (Industrial Waste); 0 (Propylene Glycols); 0 (Sewage); 3K9958V90M (Ethanol); 5965N8W85T (1,3-propanediol); 7YNJ3PO35Z (Hydrogen); PDC6A3C0OX (Glycerol)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170202
[Lr] Data última revisão:
170202
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160528
[St] Status:MEDLINE
[do] DOI:10.1080/09593330.2016.1173114


  10 / 144 MEDLINE  
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[PMID]:27120592
[Au] Autor:González-Agüero M; Tejerina Pardo L; Zamudio MS; Contreras C; Undurraga P; Defilippi BG
[Ad] Endereço:Instituto de Investigaciones Agropecuarias, INIA La Platina, Santa Rosa 11610, Santiago 8831314, Chile. maugonzalez@inia.cl.
[Ti] Título:The Unusual Acid-Accumulating Behavior during Ripening of Cherimoya (Annona cherimola Mill.) is Linked to Changes in Transcription and Enzyme Activity Related to Citric and Malic Acid Metabolism.
[So] Source:Molecules;21(5), 2016 Apr 25.
[Is] ISSN:1420-3049
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Cherimoya (Annona cherimola Mill.) is a subtropical fruit characterized by a significant increase in organic acid levels during ripening, making it an interesting model for studying the relationship between acidity and fruit flavor. In this work, we focused on understanding the balance between the concentration of organic acids and the gene expression and activity of enzymes involved in the synthesis and degradation of these metabolites during the development and ripening of cherimoya cv. "Concha Lisa". Our results showed an early accumulation of citric acid and other changes associated with the accumulation of transcripts encoding citrate catabolism enzymes. During ripening, a 2-fold increase in malic acid and a 6-fold increase in citric acid were detected. By comparing the contents of these compounds with gene expression and enzymatic activity levels, we determined that cytoplasmic NAD-dependent malate dehydrogenase (cyNAD-MDH) and mitochondrial citrate synthase (mCS) play important regulatory roles in the malic and citric acid biosynthetic pathways.
[Mh] Termos MeSH primário: Ácidos Acíclicos/metabolismo
Annona/fisiologia
Expressão Gênica
Malato Desidrogenase/genética
[Mh] Termos MeSH secundário: Annona/genética
Ácido Cítrico/metabolismo
DNA de Plantas/análise
Regulação da Expressão Gênica de Plantas
Malatos/metabolismo
Redes e Vias Metabólicas
Proteínas de Plantas/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acids, Acyclic); 0 (DNA, Plant); 0 (Malates); 0 (Plant Proteins); 2968PHW8QP (Citric Acid); 817L1N4CKP (malic acid); EC 1.1.1.37 (Malate Dehydrogenase)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170303
[Lr] Data última revisão:
170303
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160428
[St] Status:MEDLINE



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