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[PMID]:28945788
[Au] Autor:Li F; Gong A; Qiu L; Zhang W; Li J; Liu Y; Liu Y; Yuan H
[Ad] Endereço:School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing, P R, China.
[Ti] Título:Simultaneous determination of trace rare-earth elements in simulated water samples using ICP-OES with TODGA extraction/back-extraction.
[So] Source:PLoS One;12(9):e0185302, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The determination of trace rare-earth elements (REEs) can be used for the assessment of environmental pollution, and is of great significance to the study of toxicity and toxicology in animals and plants. N, N, N', N'-tetraoctyl diglycolamide (TODGA) is an environmental friendly extractant that is highly selective to REEs. In this study, an analytical method was developed for the simultaneous determination of 16 trace REEs in simulated water samples by inductively coupled plasma optical emission spectroscopy (ICP-OES). With this method, TODGA was used as the extractant to perform the liquid-liquid extraction (LLE) sample pretreatment procedure. All 16 REEs were extracted from a 3 M nitric acid medium into an organic phase by a 0.025 M TODGA petroleum ether solution. A 0.03 M ethylenediaminetetraacetic acid disodium salt (EDTA) solution was used for back-extraction to strip the REEs from the organic phase into the aqueous phase. The aqueous phase was concentrated using a vacuum rotary evaporator and the concentration of the 16 REEs was detected by ICP-OES. Under the optimum experimental conditions, the limits of detection (3σ, n = 7) for the REEs ranged from 0.0405 ng mL-1 (Nd) to 0.5038 ng mL-1 (Ho). The relative standard deviations (c = 100 ng mL-1, n = 7) were from 0.5% (Eu) to 4.0% (Tm) with a linear range of 4-1000 ng mL-1 (R2 > 0.999). The recoveries of 16 REEs ranged from 95% to 106%. The LLE-ICP-OES method established in this study has the advantages of simple operation, low detection limits, fast analysis speed and the ability to simultaneously determine 16 REEs, thereby acting as a viable alternative for the simultaneous detection of trace amounts of REEs in water samples.
[Mh] Termos MeSH primário: Metais Terras Raras/análise
Oligoelementos/análise
Poluentes Químicos da Água/análise
[Mh] Termos MeSH secundário: Monitoramento Ambiental/métodos
Glicolatos
Limite de Detecção
Extração Líquido-Líquido/métodos
Espectrofotometria/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Glycolates); 0 (Metals, Rare Earth); 0 (Trace Elements); 0 (Water Pollutants, Chemical); 0 (tetra-n-octyldiglycolamide)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170926
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0185302


  2 / 3053 MEDLINE  
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[PMID]:28538881
[Au] Autor:Al-Talib H; Al-Khateeb A; Hameed A; Murugaiah C
[Ad] Endereço:Laboratory Medical Science Cluster, Faculty of Medicine, Universiti Teknologi MARA (UiTM) - Sungai Buloh, Malaysia.
[Ti] Título:Efficacy and safety of superficial chemical peeling in treatment of active acne vulgaris.
[So] Source:An Bras Dermatol;92(2):212-216, 2017 Mar-Apr.
[Is] ISSN:1806-4841
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:Acne vulgaris is an extremely common condition affecting the pilosebaceous unit of the skin and characterized by presence of comedones, papules, pustules, nodules, cysts, which might result in permanent scars. Acne vulgaris commonly involve adolescents and young age groups. Active acne vulgaris is usually associated with several complications like hyper or hypopigmentation, scar formation and skin disfigurement. Previous studies have targeted the efficiency and safety of local and systemic agents in the treatment of active acne vulgaris. Superficial chemical peeling is a skin-wounding procedure which might cause some potentially undesirable adverse events. This study was conducted to review the efficacy and safety of superficial chemical peeling in the treatment of active acne vulgaris. It is a structured review of an earlier seven articles meeting the inclusion and exclusion criteria. The clinical assessments were based on pretreatment and post-treatment comparisons and the role of superficial chemical peeling in reduction of papules, pustules and comedones in active acne vulgaris. This study showed that almost all patients tolerated well the chemical peeling procedures despite a mild discomfort, burning, irritation and erythema have been reported; also the incidence of major adverse events was very low and easily manageable. In conclusion, chemical peeling with glycolic acid is a well-tolerated and safe treatment modality in active acne vulgaris while salicylic acid peels is a more convenient for treatment of darker skin patients and it showed significant and earlier improvement than glycolic acid.
[Mh] Termos MeSH primário: Acne Vulgar/terapia
Abrasão Química/métodos
Glicolatos/uso terapêutico
Ceratolíticos/uso terapêutico
Ácido Salicílico/uso terapêutico
[Mh] Termos MeSH secundário: Abrasão Química/efeitos adversos
Eritema/etiologia
Seres Humanos
Salicilatos
Resultado do Tratamento
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Glycolates); 0 (Keratolytic Agents); 0 (Salicylates); 0WT12SX38S (glycolic acid); O414PZ4LPZ (Salicylic Acid)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171006
[Lr] Data última revisão:
171006
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170525
[St] Status:MEDLINE


  3 / 3053 MEDLINE  
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[PMID]:28429316
[Au] Autor:Nakazawa M
[Ad] Endereço:Faculty of Life and Environmental Sciences, Osaka Prefecture University, Sakai, Osaka, Japan. mami@biochem.osakafu-u.ac.jp.
[Ti] Título:C2 metabolism in Euglena.
[So] Source:Adv Exp Med Biol;979:39-45, 2017.
[Is] ISSN:0065-2598
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Euglenoids are able to assimilate fatty acids and alcohols with various carbon-chain lengths, and ethanol is known to be one of the best carbon sources to support the growth of Euglena gracilis. Ethanol is first oxidized to acetate by the sequential reactions of alcohol dehydrogenase and acetaldehyde dehydrogenase in the mitochondria, and then converted to acetyl coenzyme A (acetyl-CoA). Acetyl-CoA is metabolized through the glyoxylate cycle which is a modified tricarboxylic acid (TCA) cycle in which isocitrate lyase (ICL) and malate synthase (MS) function to bypass the two decarboxylation steps of the TCA cycle, enabling the net synthesis of carbohydrates from C2 compounds. ICL and MS form a unique bifunctional enzyme localized in Euglena mitochondria, not in glyoxysome as in other eukaryotes. The unique glyoxylate and glycolate metabolism during photorespiration is also discussed in this chapter.
[Mh] Termos MeSH primário: Ácido Acético/metabolismo
Etanol/metabolismo
Euglena/metabolismo
Glicolatos/metabolismo
Glioxilatos/metabolismo
[Mh] Termos MeSH secundário: Ciclo do Ácido Cítrico/fisiologia
Mitocôndrias/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Glycolates); 0 (Glyoxylates); 0WT12SX38S (glycolic acid); 3K9958V90M (Ethanol); JQ39C92HH6 (glyoxylic acid); Q40Q9N063P (Acetic Acid)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171024
[Lr] Data última revisão:
171024
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170422
[St] Status:MEDLINE
[do] DOI:10.1007/978-3-319-54910-1_3


  4 / 3053 MEDLINE  
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[PMID]:28424880
[Au] Autor:Isbister KM; Lamb EG; Stewart KJ
[Ad] Endereço:Department of Plant Sciences, University of Saskatchewan, 51 Campus Drive, Saskatoon, S7N 5A8, SK, Canada.
[Ti] Título:Herbicide Toxicity Testing with Non-Target Boreal Plants: The Sensitivity of Achillea millefolium L. and Chamerion angustifolium L. to Triclopyr and Imazapyr.
[So] Source:Environ Manage;60(1):136-156, 2017 Jul.
[Is] ISSN:1432-1009
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Terrestrial plant toxicity tests were conducted to determine the sensitivity of two boreal plants, yarrow (Achillea millefolium L.) and fireweed (Chamerion angustifolium L.), to the herbicides imazapyr and triclopyr. Both plants are common non-target species on northern powerline rights-of-way where the impacts of proposed herbicide applications are of concern. In the vegetative vigour test, triclopyr foliar spray caused extensive damage to A. millefolium at <50% of the maximum field application rate (inhibition concentration (IC) = 1443.8 g a.i. ha ) and was lethal to C. angustifolium at the lowest dose tested (1210.9 g a.i. ha ). Both species demonstrated extremely high sensitivity to imazapyr foliar spray: IC = 8.29 g a.i. ha and 4.82 g a.i. ha (<1.5% of the maximum field rate). The seedling emergence and seedling growth tests were conducted in the organic horizon of five boreal soils. Few differences in herbicide bioavailability between soils were detected. Triclopyr limited growth of A. millefolium, C. angustifolium and standard test species Calamagrostis canadensis at low levels (most IC estimates between 2-20 µg g ). For imazapyr, IC estimates could not be calculated as there was >75% inhibition of endpoints at the lowest doses of ~2 µg g . A foliar application of triclopyr or imazapyr for woody species control would likely cause significant damage to boreal non-target plants. The high sensitivity of both species to herbicide residues in soil indicates long term impacts are dependent on herbicide degradation rates in northern conditions. A. millefolium performed well and is recommended for use in toxicity testing relevant to boreal regions.
[Mh] Termos MeSH primário: Achillea/efeitos dos fármacos
Glicolatos/toxicidade
Herbicidas/toxicidade
Imidazóis/toxicidade
Niacina/análogos & derivados
Onagraceae/efeitos dos fármacos
Poluentes do Solo/toxicidade
[Mh] Termos MeSH secundário: Achillea/crescimento & desenvolvimento
Clima Frio
Niacina/toxicidade
Onagraceae/crescimento & desenvolvimento
Projetos Piloto
Plântulas/efeitos dos fármacos
Plântulas/crescimento & desenvolvimento
Solo/química
Especificidade da Espécie
Testes de Toxicidade
Yukon
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Glycolates); 0 (Herbicides); 0 (Imidazoles); 0 (Soil); 0 (Soil Pollutants); 2679MF687A (Niacin); 787MX0M5A6 (imazapyr); MV06PHJ6I0 (triclopyr)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170421
[St] Status:MEDLINE
[do] DOI:10.1007/s00267-017-0867-7


  5 / 3053 MEDLINE  
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[PMID]:28351772
[Au] Autor:Sprando RL; Mossoba ME; Black T; Keltner Z; Vohra S; Olejnik N; Toomer H; Stine C; Evans E; Sprando JL; Ferguson M
[Ad] Endereço:Center for Food Safety & Applied Nutrition, U.S. Food & Drug Administration, Laurel, MD 20708, United States. Electronic address: Robert.sprando@fda.hhs.gov.
[Ti] Título:28-day repeated dose response study of diglycolic acid: Renal and hepatic effects.
[So] Source:Food Chem Toxicol;106(Pt A):558-567, 2017 Aug.
[Is] ISSN:1873-6351
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The acute oral toxicity of diglycolic acid (DGA) was evaluated. Groups of female rats (n = 8 rats/group) received 28 consecutive daily single doses of 0.3, 1.0, 3.0, 10.0, 30.0, 100.0 or 300.0 mg DGA/kg body weight by gastric intubation. One group of animals served as vehicle control. Tissues and blood serum were collected at necropsy on day 29. Select organs were weighed and fixed in formalin for histopathological analysis. Animals from the 300 mg/kg bw dose group were removed from the study after 5 consecutive days of treatment as a consequence of adverse treatment related effects. The animals in the remaining treatment groups survived the exposure period. No adverse clinical signs were observed throughout the exposure period in the surviving animals. No significant differences from controls were observed for feed and fluid consumption or body weight gain in the surviving animals. Lesions were observed in the kidneys, liver, stomach, intestine, thymus, spleen and bone marrow in rats from the 300 mg/kg dose group and signs of renal tubular regeneration were observed only in the 100 mg/kg dose group. These results suggest that high levels of pure DGA would need to be consumed before renal and other forms of organ toxicity are observed.
[Mh] Termos MeSH primário: Glicolatos/toxicidade
Rim/efeitos dos fármacos
Fígado/efeitos dos fármacos
[Mh] Termos MeSH secundário: Estruturas Animais/efeitos dos fármacos
Estruturas Animais/patologia
Animais
Relação Dose-Resposta a Droga
Feminino
Glicolatos/administração & dosagem
Rim/patologia
Fígado/patologia
Ratos
Ratos Sprague-Dawley
Testes de Toxicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Glycolates); S4651635E0 (diglycolic acid)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170927
[Lr] Data última revisão:
170927
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170330
[St] Status:MEDLINE


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[PMID]:28266799
[Au] Autor:Xu G; Wang A; Pang J; Zhao X; Xu J; Lei N; Wang J; Zheng M; Yin J; Zhang T
[Ad] Endereço:State Key Laboratory of Catalysis, iChEM, Collaborative Innovation Center of Chemistry for Energy Materials), Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian, 116023, P. R. China.
[Ti] Título:Chemocatalytic Conversion of Cellulosic Biomass to Methyl Glycolate, Ethylene Glycol, and Ethanol.
[So] Source:ChemSusChem;10(7):1390-1394, 2017 Apr 10.
[Is] ISSN:1864-564X
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Production of chemicals and fuels from renewable cellulosic biomass is important for the creation of a sustainable society, and it critically relies on the development of new and efficient transformation routes starting from cellulose. Here, a chemocatalytic conversion route from cellulosic biomass to methyl glycolate (MG), ethylene glycol (EG), and ethanol (EtOH) is reported. By using a tungsten-based catalyst, cellulose is converted into MG with a yield as high as 57.7 C % in a one-pot reaction in methanol at 240 °C and 1 MPa O , and the obtained MG can be easily separated by distillation. Afterwards, it can be nearly quantitatively converted to EG at 200 °C and to EtOH at 280 °C with a selectivity of 50 % through hydrogenation over a Cu/SiO catalyst. By this approach, the fine chemical MG, the bulk chemical EG, and the fuel additive EtOH can all be efficiently produced from renewable cellulosic materials, thus providing a new pathway towards mitigating the dependence on fossil resources.
[Mh] Termos MeSH primário: Biomassa
Celulose/química
Etanol/química
Etilenoglicol/química
Glicolatos/química
[Mh] Termos MeSH secundário: Catálise
Hidrogenação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Glycolates); 3K9958V90M (Ethanol); 9004-34-6 (Cellulose); FC72KVT52F (Ethylene Glycol)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170606
[Lr] Data última revisão:
170606
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170308
[St] Status:MEDLINE
[do] DOI:10.1002/cssc.201601714


  7 / 3053 MEDLINE  
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[PMID]:28247236
[Au] Autor:Schmitz J; Srikanth NV; Hüdig M; Poschmann G; Lercher MJ; Maurino VG
[Ad] Endereço:Institute of Developmental and Molecular Biology of Plants, Plant Molecular Physiology and Biotechnology Group, Heinrich Heine University, Universitätsstraße 1, and Cluster of Excellence on Plant Sciences (CEPLAS), 40225, Düsseldorf, Germany.
[Ti] Título:The ancestors of diatoms evolved a unique mitochondrial dehydrogenase to oxidize photorespiratory glycolate.
[So] Source:Photosynth Res;132(2):183-196, 2017 May.
[Is] ISSN:1573-5079
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Like other oxygenic photosynthetic organisms, diatoms produce glycolate, a toxic intermediate, as a consequence of the oxygenase activity of Rubisco. Diatoms can remove glycolate through excretion and through oxidation as part of the photorespiratory pathway. The diatom Phaeodactylum tricornutum encodes two proteins suggested to be involved in glycolate metabolism: PtGO1 and PtGO2. We found that these proteins differ substantially from the sequences of experimentally characterized proteins responsible for glycolate oxidation in other species, glycolate oxidase (GOX) and glycolate dehydrogenase. We show that PtGO1 and PtGO2 are the only sequences of P. tricornutum homologous to GOX. Our phylogenetic analyses indicate that the ancestors of diatoms acquired PtGO1 during the proposed first secondary endosymbiosis with a chlorophyte alga, which may have previously obtained this gene from proteobacteria. In contrast, PtGO2 is orthologous to an uncharacterized protein in Galdieria sulphuraria, consistent with its acquisition during the secondary endosymbiosis with a red alga that gave rise to the current plastid. The analysis of amino acid residues at conserved positions suggests that PtGO2, which localizes to peroxisomes, may use substrates other than glycolate, explaining the lack of GOX activity we observe in vitro. Instead, PtGO1, while only very distantly related to previously characterized GOX proteins, evolved glycolate-oxidizing activity, as demonstrated by in gel activity assays and mass spectrometry analysis. PtGO1 localizes to mitochondria, consistent with previous suggestions that photorespiration in diatoms proceeds in these organelles. We conclude that the ancestors of diatoms evolved a unique alternative to oxidize photorespiratory glycolate: a mitochondrial dehydrogenase homologous to GOX able to use electron acceptors other than O .
[Mh] Termos MeSH primário: Diatomáceas/enzimologia
Diatomáceas/metabolismo
Glicolatos/metabolismo
Mitocôndrias/enzimologia
Oxirredutases/genética
[Mh] Termos MeSH secundário: Oxirredutases do Álcool/genética
Oxirredutases do Álcool/metabolismo
Mitocôndrias/metabolismo
Oxirredução
Oxirredutases/metabolismo
Fotossíntese/genética
Fotossíntese/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Glycolates); 0WT12SX38S (glycolic acid); EC 1.- (Oxidoreductases); EC 1.1.- (Alcohol Oxidoreductases); EC 1.1.3.15 (glycollate oxidase)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171121
[Lr] Data última revisão:
171121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170302
[St] Status:MEDLINE
[do] DOI:10.1007/s11120-017-0355-1


  8 / 3053 MEDLINE  
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[PMID]:28231455
[Au] Autor:Nday CM; Halevas E; Tsiaprazi-Stamou A; Eleftheriadou D; Hatzidimitriou A; Jackson G; Reid D; Salifoglou A
[Ad] Endereço:Laboratory of Inorganic Chemistry, Department of Chemical Engineering, Aristotle University of Thessaloniki, Thessaloniki 54124, Greece.
[Ti] Título:Synthetic investigation, physicochemical characterization and antibacterial evaluation of ternary Bi(III) systems with hydroxycarboxylic acid and aromatic chelator substrates.
[So] Source:J Inorg Biochem;170:98-108, 2017 May.
[Is] ISSN:1873-3344
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Due to its physical and chemical properties, bismuth (Bi(III)) is widely used in the treatment of several gastrointestinal and skin diseases, and infections caused by bacteria. Herein, its known antimicrobial potential was taken into consideration in the synthesis of two new hybrid ternary materials of Bi(III) with the physiological α-hydroxycarboxylic glycolic acid and 1,10-phenanthroline (phen), [Bi (C H O ) (C H O )(NO )] nH O (1) and [Bi(C H N )(NO ) ](C H N ) (2), aiming at improving its antibacterial properties. Their physicochemical characterization was carried out through elemental analysis, FT-IR, atomic absorption spectroscopy, single crystal X-ray diffraction, thermogravimetric analysis (TGA), photoluminescence, and C MAS-NMR techniques. The antimicrobial activity of the title complexes was directly linked to Bi(III) coordination environment and the incipient aqueous chemistry. For their antibacterial assessment, minimum inhibitory concentration (MIC), zone of inhibition (ZOI), and bacteriostatic-bacteriocidal activity were determined in various Gram positive (Staphylococcus aureus, Bacillus subtilis and Bacillus cereus) and Gram negative (Escherichia coli and Xanthomonas campestris) bacterial cultures, in reference to a positive control (ampicillin), encompassing further comparisons with literature data. The findings reveal that the new hybrid bismuth materials have significant antimicrobial effects against the employed bacteria. Specifically, 2 exhibits better antimicrobial properties than free Bi(NO ) and phen. On the other hand, 1 is bacteriostatic toward four microorganisms except X. campestris, with 2 being bacteriocidal toward four microorganisms except B. cereus. Collectively, the new hybrid, well-defined, and two of the rarely crystallographically characterized Bi(III) materials a) exhibit properties reflecting their physicochemical nature and reactivity, and b) are expected to contribute to the development of efficient metallodrugs against drug-resistant bacterial infections.
[Mh] Termos MeSH primário: Antibacterianos
Bactérias/crescimento & desenvolvimento
Bismuto
Glicolatos
[Mh] Termos MeSH secundário: Antibacterianos/síntese química
Antibacterianos/química
Antibacterianos/farmacologia
Bismuto/química
Bismuto/farmacologia
Quelantes/síntese química
Quelantes/química
Quelantes/farmacologia
Glicolatos/síntese química
Glicolatos/química
Glicolatos/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Chelating Agents); 0 (Glycolates); 0WT12SX38S (glycolic acid); U015TT5I8H (Bismuth)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170918
[Lr] Data última revisão:
170918
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170224
[St] Status:MEDLINE


  9 / 3053 MEDLINE  
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[PMID]:28202596
[Au] Autor:Guan X; Okazaki Y; Lithio A; Li L; Zhao X; Jin H; Nettleton D; Saito K; Nikolau BJ
[Ad] Endereço:Department of Biochemistry, Biophysics, and Molecular Biology (X.G., H.J., B.J.N.), National Science Foundation Engineering Research Center for Biorenewable Chemicals (X.G., B.J.N.), Department of Statistics (A.L., D.N.), Department of Genetics, Development, and Cellular Biology (L.L.), Laurence H.
[Ti] Título:Discovery and Characterization of the 3-Hydroxyacyl-ACP Dehydratase Component of the Plant Mitochondrial Fatty Acid Synthase System.
[So] Source:Plant Physiol;173(4):2010-2028, 2017 Apr.
[Is] ISSN:1532-2548
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We report the characterization of the Arabidopsis ( ) 3-hydroxyacyl-acyl carrier protein dehydratase (mtHD) component of the mitochondrial fatty acid synthase (mtFAS) system, encoded by AT5G60335. The mitochondrial localization and catalytic capability of mtHD were demonstrated with a green fluorescent protein transgenesis experiment and by in vivo complementation and in vitro enzymatic assays. RNA interference (RNAi) knockdown lines with reduced expression exhibit traits typically associated with mtFAS mutants, namely a miniaturized morphological appearance, reduced lipoylation of lipoylated proteins, and altered metabolomes consistent with the reduced catalytic activity of lipoylated enzymes. These alterations are reversed when mutant plants are grown in a 1% CO atmosphere, indicating the link between mtFAS and photorespiratory deficiency due to the reduced lipoylation of glycine decarboxylase. In vivo biochemical feeding experiments illustrate that sucrose and glycolate are the metabolic modulators that mediate the alterations in morphology and lipid accumulation. In addition, both and mutants exhibit reduced accumulation of 3-hydroxytetradecanoic acid (i.e. a hallmark of lipid A-like molecules) and abnormal chloroplastic starch granules; these changes are not reversible by the 1% CO atmosphere, demonstrating two novel mtFAS functions that are independent of photorespiration. Finally, RNA sequencing analysis revealed that and mutants are nearly equivalent to each other in altering the transcriptome, and these analyses further identified genes whose expression is affected by a functional mtFAS system but independent of photorespiratory deficiency. These data demonstrate the nonredundant nature of the mtFAS system, which contributes unique lipid components needed to support plant cell structure and metabolism.
[Mh] Termos MeSH primário: Proteínas de Arabidopsis/metabolismo
Arabidopsis/enzimologia
Ácido Graxo Sintase Tipo II/metabolismo
Ácido Graxo Sintases/metabolismo
Hidroliases/metabolismo
Mitocôndrias/enzimologia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Arabidopsis/genética
Proteínas de Arabidopsis/genética
Western Blotting
Dióxido de Carbono/metabolismo
Ácido Graxo Sintase Tipo II/genética
Ácido Graxo Sintases/genética
Regulação da Expressão Gênica de Plantas
Glicolatos/metabolismo
Proteínas de Fluorescência Verde/genética
Proteínas de Fluorescência Verde/metabolismo
Hidroliases/genética
Metabolômica/métodos
Microscopia Confocal
Microscopia Eletrônica de Transmissão
Mitocôndrias/ultraestrutura
Mutação
Ácidos Mirísticos/metabolismo
Plantas Geneticamente Modificadas
Interferência de RNA
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Análise de Sequência de RNA/métodos
Homologia de Sequência de Aminoácidos
Sacarose/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arabidopsis Proteins); 0 (Glycolates); 0 (Myristic Acids); 0WT12SX38S (glycolic acid); 142M471B3J (Carbon Dioxide); 147336-22-9 (Green Fluorescent Proteins); 1961-72-4 (beta-hydroxymyristic acid); 57-50-1 (Sucrose); EC 2.3.1.85 (Fatty Acid Synthases); EC 4.2.1.- (Hydro-Lyases); EC 4.2.1.59 (3-hydroxyacyl-(acyl-carrier-protein) dehydratase); EC 6.- (Fatty Acid Synthase, Type II)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170217
[St] Status:MEDLINE
[do] DOI:10.1104/pp.16.01732


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[PMID]:28150506
[Au] Autor:Føreid S; Gadeholt G
[Ad] Endereço:a Department of Pharmacology , Oslo University Hospital , Oslo , Norway.
[Ti] Título:Beta-hydroxybutyrate and pyroglutamate can be included in a rapid GC-MS screening method for differential diagnosis of metabolic acidosis.
[So] Source:Scand J Clin Lab Invest;77(2):149-152, 2017 Apr.
[Is] ISSN:1502-7686
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A rapid gas chromatographic mass spectrometric method for measuring anions associated with acute anion gap metabolic acidosis is described. The method is an extension of a previous method. The method quantifies glycolic acid, beta-hydroxybutyric acid with good linearity and pyroglutamic acid with a reproducible curvature relation between 1 and 20 mmol/L and can help the clinician distinguish effectively between ethylene glycol poisoning, alcoholic and diabetic ketoacidosis and cysteine deficiency so early that it will have clinical consequences.
[Mh] Termos MeSH primário: Ácido 3-Hidroxibutírico/sangue
Cetoacidose Diabética/diagnóstico
Cromatografia Gasosa-Espectrometria de Massas/métodos
Glicolatos/sangue
Cetose/diagnóstico
Ácido Pirrolidonocarboxílico/sangue
[Mh] Termos MeSH secundário: Biomarcadores/sangue
Calibragem
Cisteína/deficiência
Cetoacidose Diabética/sangue
Diagnóstico Diferencial
Etilenoglicol/envenenamento
Seres Humanos
Cetose/sangue
Reprodutibilidade dos Testes
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 0 (Glycolates); 0WT12SX38S (glycolic acid); FC72KVT52F (Ethylene Glycol); K848JZ4886 (Cysteine); SZB83O1W42 (Pyrrolidonecarboxylic Acid); TZP1275679 (3-Hydroxybutyric Acid)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170323
[Lr] Data última revisão:
170323
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170203
[St] Status:MEDLINE
[do] DOI:10.1080/00365513.2016.1278261



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