Base de dados : MEDLINE
Pesquisa : D02.241.081.018.386.682.300 [Categoria DeCS]
Referências encontradas : 2138 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 214 ir para página                         

  1 / 2138 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28479418
[Au] Autor:Valdez BC; Hassan M; Andersson BS
[Ad] Endereço:Department of Stem Cell Transplantation and Cellular Therapy, The University of Texas M.D. Anderson Cancer Center, Houston, TX. Electronic address: bvaldez@mdanderson.org.
[Ti] Título:Development of an assay for cellular efflux of pharmaceutically active agents and its relevance to understanding drug interactions.
[So] Source:Exp Hematol;52:65-71, 2017 Aug.
[Is] ISSN:1873-2399
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Drug interactions may dictate the failure or success of a treatment. Patients undergoing hematopoietic stem cell transplantation (HSCT) are exposed to various types of drugs, and understanding how these drugs interact is of the utmost importance. The pharmacokinetics of busulfan, melphalan, and cyclophosphamide, drugs commonly used for HSCT, are known to be affected by a variety of other drugs with differing molecular structures. We hypothesized that these structurally unrelated drugs affect the transport of DNA-alkylating agents. To test this hypothesis, we developed a flow cytometry assay that used 5-carboxyfluorescein diacetate acetoxymethyl ester, which is cleaved by nonspecific intracellular esterases to 5-carboxyfluorescein (5-CF), a fluorescent ligand for the drug transporter MRP1. A decreased 5-CF efflux in the presence of a test compound suggests competitive inhibition. We demonstrated that chlorambucil, 4-hydroperoxycyclophosphamide, ketoconazole, ethacrynic acid, everolimus, and sirolimus strongly inhibited 5-CF efflux in lymphoma and leukemia cell lines. The efflux of these drugs partially depends on the glutathione (GSH) level, and their cytotoxicity is synergistic with inhibited GSH synthesis. This is consistent with the hypothesis that their GSH-conjugated products are ligands of a common cellular drug transporter. Our results may explain clinical observations on the effects of various drugs on the pharmacokinetics and pharmacodynamics of alkylating agents, and the assay may be used to deduce interaction mechanisms of drugs transported by a common system.
[Mh] Termos MeSH primário: Ciclofosfamida/farmacologia
Interações Medicamentosas
Citometria de Fluxo/métodos
Fluoresceínas/metabolismo
[Mh] Termos MeSH secundário: Antineoplásicos Alquilantes/farmacocinética
Antineoplásicos Alquilantes/farmacologia
Transporte Biológico/efeitos dos fármacos
Bussulfano/farmacocinética
Bussulfano/farmacologia
Linhagem Celular Tumoral
Sobrevivência Celular/efeitos dos fármacos
Clorambucila/farmacocinética
Clorambucila/farmacologia
Ciclofosfamida/análogos & derivados
Ciclofosfamida/farmacocinética
Ácido Etacrínico/farmacocinética
Ácido Etacrínico/farmacologia
Everolimo/farmacocinética
Everolimo/farmacologia
Fluoresceínas/química
Seres Humanos
Cetoconazol/farmacocinética
Cetoconazol/farmacologia
Melfalan/farmacocinética
Melfalan/farmacologia
Proteínas Associadas à Resistência a Múltiplos Medicamentos/metabolismo
Reprodutibilidade dos Testes
Sirolimo/farmacocinética
Sirolimo/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (5-carboxyfluorescein diacetate acetoxymethyl ester); 0 (Antineoplastic Agents, Alkylating); 0 (Fluoresceins); 0 (Multidrug Resistance-Associated Proteins); 18D0SL7309 (Chlorambucil); 76823-03-5 (4-carboxyfluorescein); 8N3DW7272P (Cyclophosphamide); 9HW64Q8G6G (Everolimus); G1LN9045DK (Busulfan); M5DP350VZV (Ethacrynic Acid); Q41OR9510P (Melphalan); R9400W927I (Ketoconazole); U880A4FUDA (perfosfamide); W36ZG6FT64 (Sirolimus); Y49M64GZ4Q (multidrug resistance-associated protein 1)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171023
[Lr] Data última revisão:
171023
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170509
[St] Status:MEDLINE


  2 / 2138 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
[PMID]:28179301
[Au] Autor:Messina CS; Weiher H; Schmidt-Wolf IG
[Ad] Endereço:Center for Integrated Oncology (CIO), Department of Internal Medicine III, University Hospital Bonn, Rheinische Friedrich Wilhelm University Bonn, Bonn, Germany.
[Ti] Título:Targeting Prostate Cancer with a Combination of WNT Inhibitors and a Bi-functional Peptide.
[So] Source:Anticancer Res;37(2):555-559, 2017 02.
[Is] ISSN:1791-7530
[Cp] País de publicação:Greece
[La] Idioma:eng
[Ab] Resumo:BACKGROUND/AIM: Prostate cancer is the most common cancer in the Western world. A bi-functional peptide was combined with wingless-related integration site (WNT) inhibitors to determine if there is an additive therapeutic effect when they are used against prostate cancer, since their efficacy has already been proven when used alone. MATERIALS AND METHODS: A bi-functional peptide (TP-LYT) was designed with a target domain (LTVSPWY) and a lytic domain (KLAKLAK) , and a second peptide with the same lytic domain but a random sequence instead of the target domain was used as a negative control. Two different WNT inhibitors were used, ethacrynic acid and ciclopiroxolamine. They were tested on prostate cancer cells using the WST-8 assay. RESULTS: A synergistic effect of peptides and WNT inhibitors was demonstrated, increasing the toxicity against cancer cells. CONCLUSION: Our findings potentially allow safer treatment since lower concentrations of WNT inhibitors can be used in combination with this bi-functional peptide.
[Mh] Termos MeSH primário: Protocolos de Quimioterapia Combinada Antineoplásica/farmacologia
Ácido Etacrínico/farmacologia
Oligopeptídeos/farmacologia
Peptídeos/farmacologia
Neoplasias da Próstata/tratamento farmacológico
Piridonas/farmacologia
Proteínas Wnt/antagonistas & inibidores
[Mh] Termos MeSH secundário: Sinergismo Farmacológico
Ácido Etacrínico/administração & dosagem
Seres Humanos
Masculino
Terapia de Alvo Molecular
Oligopeptídeos/administração & dosagem
Peptídeos/administração & dosagem
Neoplasias da Próstata/metabolismo
Domínios Proteicos
Piridonas/administração & dosagem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (KLA peptide); 0 (Oligopeptides); 0 (Peptides); 0 (Pyridones); 0 (Wnt Proteins); 0 (leucyl-threonyl-valyl-seryl-prolyl-tryptophyl-tyrosine); 19W019ZDRJ (ciclopirox); M5DP350VZV (Ethacrynic Acid)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170213
[Lr] Data última revisão:
170213
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170210
[St] Status:MEDLINE


  3 / 2138 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
[PMID]:27466493
[Au] Autor:Bernig T; Ritz S; Brodt G; Volkmer I; Staege MS
[Ad] Endereço:Department of Pediatrics, Martin Luther University Halle-Wittenberg, Halle, Germany.
[Ti] Título:Glutathione-S-transferases and Chemotherapy Resistance of Hodgkin's Lymphoma Cell Lines.
[So] Source:Anticancer Res;36(8):3905-15, 2016 Aug.
[Is] ISSN:1791-7530
[Cp] País de publicação:Greece
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Glutathione-S-transferases (GSTs) are associated with multidrug resistance of tumor cells and are involved in drug detoxification and control of apoptosis. We analyzed the impact of GSTs on apoptosis of Hodgkin's lymphoma (HL) cells. MATERIALS AND METHODS: Expression of GST isoforms in HL cell lines was assessed by analysis of DNA microarray data and quantitative reverse transcription-polymerase chain reaction (qRT-PCR). The impact of the GST inhibitor ethacrynic acid (EA) on HL cell survival was analyzed in vitro. RESULTS: DNA microarray analysis and qRT-PCR analysis demonstrated higher expression of GST isoforms in chemoresistant HL cells. Therefore, GSTs may contribute to chemoresistance of HL cells. Incubation of GST-expressing chemoresistant L-1236 HL cells with EA significantly enhanced the activity of cisplatin against these cells. CONCLUSION: Our data suggest that the combined treatment with chemotherapy and GST inhibitors such as EA might be an interesting option for patients with chemoresistant HL.
[Mh] Termos MeSH primário: Resistência a Medicamentos Antineoplásicos/genética
Glutationa Transferase/biossíntese
Doença de Hodgkin/tratamento farmacológico
Doença de Hodgkin/genética
[Mh] Termos MeSH secundário: Apoptose/genética
Linhagem Celular Tumoral
Cisplatino/uso terapêutico
Ácido Etacrínico/administração & dosagem
Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos
Glutationa/metabolismo
Glutationa Transferase/antagonistas & inibidores
Glutationa Transferase/genética
Doença de Hodgkin/patologia
Seres Humanos
Análise de Sequência com Séries de Oligonucleotídeos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
EC 2.5.1.18 (Glutathione Transferase); GAN16C9B8O (Glutathione); M5DP350VZV (Ethacrynic Acid); Q20Q21Q62J (Cisplatin)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170125
[Lr] Data última revisão:
170125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160729
[St] Status:MEDLINE


  4 / 2138 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27448922
[Au] Autor:Mignani S; El Brahmi N; El Kazzouli S; Eloy L; Courilleau D; Caron J; Bousmina MM; Caminade AM; Cresteil T; Majoral JP
[Ad] Endereço:Laboratoire de Chimie et de Biochimie pharmacologiques et toxicologique, CNRS UMR 860, Université Paris Descartes, PRES Sorbonne Paris Cité, 45, rue des Saints Pères, 75006, Paris, France. Electronic address: serge.mignani@parisdescartes.fr.
[Ti] Título:A novel class of ethacrynic acid derivatives as promising drug-like potent generation of anticancer agents with established mechanism of action.
[So] Source:Eur J Med Chem;122:656-673, 2016 Oct 21.
[Is] ISSN:1768-3254
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:The well-known diuretic Ethacrynic acid (EA, Edecrin), showing low anti-proliferative activities, was chemically modified at different positions. The new EA derivatives have been tested in vitro in anti-proliferative assays on both tumor KB (epidermal carcinoma) and leukemia HL60 (promyelocytic) cells suitable targets for anticancer activity. Reduction of the α-ß double bond of EA completely abolished anti-cancer activities, whereas introduction of either 2-(4-substituted phenyl)ethanamine (series A) or 4-(4-substituted phenyl)piperazine (series B) moieties generated compounds showing moderate to strong anti-proliferative activities against human cancer cell lines. Several substitutions on the phenyl of these two moieties are tolerated. The mechanism of action of the EA derivatives prepared in this study is more complex than the inhibition of glutathione S-transferase π ascribed as unique effect to EA and might help to overcome tumor resistances.
[Mh] Termos MeSH primário: Antineoplásicos/química
Antineoplásicos/farmacologia
Ácido Etacrínico/química
Ácido Etacrínico/farmacologia
[Mh] Termos MeSH secundário: Caspase 3/metabolismo
Caspase 7/metabolismo
Ciclo Celular/efeitos dos fármacos
Proliferação Celular/efeitos dos fármacos
Desenho de Drogas
Ativação Enzimática/efeitos dos fármacos
Glutationa S-Transferase pi/antagonistas & inibidores
Células HL-60
Seres Humanos
Células KB
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents); EC 2.5.1.18 (Glutathione S-Transferase pi); EC 3.4.22.- (Caspase 3); EC 3.4.22.- (Caspase 7); M5DP350VZV (Ethacrynic Acid)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170807
[Lr] Data última revisão:
170807
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160725
[St] Status:MEDLINE


  5 / 2138 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
[PMID]:27363264
[Au] Autor:Zhang N; Jin G; Jin Z; Liu B; Peng B; Gao N; Hu Y; Tang L
[Ad] Endereço:Department of Pathophysiology, Laboratory of Stem Cell and Tissue Engineering, Chongqing Medical University, Chongqing 400016, China.
[Ti] Título:[Combination of TLR7 agonist T7-ethacrynic acid conjugate with ROR1 has a stronger anti-breast cancer effect].
[So] Source:Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi;32(7):876-80, 2016 Jul.
[Is] ISSN:1007-8738
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:Objective To investigate the synergistic anti-breast cancer effect of Toll-like receptor 7 agonist T7-ethacrynic acid conjugate (T7-EA) in combination with receptor-tyrosine-kinase-like orphan receptor 1 (ROR1). Methods ROR1 cytotoxic T lymphocyte (CTL) epitope was predicted using Syfpeithi online software. Mouse spleen lymphocytes and bone marrow dendritic cells (DCs) were separately stimulated with 4 µmol/L T7-EA and 4 µmol/L ROR1 alone or in combination. ELISA assay was used to measure the levels of interferon-γ (IFN-γ), interleukin 12 (IL-12) and tumor necrosis factor-α (TNF-α). Xenograft model was established via subcutaneous injection of mouse breast cancer 4T1 cells. The mice were weekly treated through intraperitoneal administration of 3 mg/kg T7-EA, 15 mg/kg ROR1 or the combination of T7-EA and ROR1. After four rounds of treatment, tumor tissues were weighed. Serum level of anti-4T1 tumor protein IgG was measured by ELISA. Specific CTL activity was detected by lactate dehydrogenase (LDH) assay. Results The peptide PYCDETSSV was chosen as an antigen epitope of breast cancer. The T7-EA highly activated in vitro lymphocytes in a dose-dependent manner, which wasn't affected by other relevant peptides. The combination of T7-EA and ROR1 stimulated the secretion of IFN-γ and IL-12 by lymphocytes and TNF-α by bone marrow DCs. The growth of tumor in vivo was significantly inhibited by T7-EA combined with ROR1 compared with T7-EA or ROR1 alone. The specific CTL activity triggered by T7-EA combined with ROR1 was much stronger than that triggered by T7-EA or ROR1 alone. The titer of anti-4T1 tumor protein IgG induced by T7-EA combined with ROR1 was higher than that induced by T7-EA or ROR1. Conclusion The combination of T7-EA and ROR1 has a better killing effect on breast cancer.
[Mh] Termos MeSH primário: Vacinas Anticâncer/imunologia
Ácido Etacrínico/imunologia
Neoplasias Mamárias Experimentais/imunologia
Receptores Órfãos Semelhantes a Receptor Tirosina Quinase/imunologia
Receptor 7 Toll-Like/imunologia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Vacinas Anticâncer/farmacologia
Linhagem Celular Tumoral
Células Dendríticas/efeitos dos fármacos
Células Dendríticas/imunologia
Células Dendríticas/metabolismo
Ensaio de Imunoadsorção Enzimática
Feminino
Imunoglobulina G/sangue
Imunoglobulina G/imunologia
Interferon gama/imunologia
Interferon gama/metabolismo
Interleucina-12/imunologia
Interleucina-12/metabolismo
Linfócitos/efeitos dos fármacos
Linfócitos/imunologia
Linfócitos/metabolismo
Neoplasias Mamárias Experimentais/metabolismo
Neoplasias Mamárias Experimentais/prevenção & controle
Camundongos Endogâmicos BALB C
Receptores Órfãos Semelhantes a Receptor Tirosina Quinase/metabolismo
Fatores de Tempo
Receptor 7 Toll-Like/agonistas
Receptor 7 Toll-Like/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cancer Vaccines); 0 (Immunoglobulin G); 0 (TLR7 protein, human); 0 (Toll-Like Receptor 7); 187348-17-0 (Interleukin-12); 82115-62-6 (Interferon-gamma); EC 2.7.10.1 (ROR1 protein, human); EC 2.7.10.1 (Receptor Tyrosine Kinase-like Orphan Receptors); M5DP350VZV (Ethacrynic Acid)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170104
[Lr] Data última revisão:
170104
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160702
[St] Status:MEDLINE


  6 / 2138 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27156773
[Au] Autor:Punganuru SR; Mostofa AGM; Madala HR; Basak D; Srivenugopal KS
[Ad] Endereço:Department of Biomedical Sciences and Cancer Biology Center, School of Pharmacy, Texas Tech University Health Sciences Center, 1406 S. Coulter Dr., Amarillo, TX 79106, USA.
[Ti] Título:Potent anti-proliferative actions of a non-diuretic glucosamine derivative of ethacrynic acid.
[So] Source:Bioorg Med Chem Lett;26(12):2829-2833, 2016 06 15.
[Is] ISSN:1464-3405
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Ethacrynic acid (EA), a known inhibitor of the neoplastic marker glutathione S-transferase P1 and other GSTs, exerts a weak antiproliferative activity against human cancer cells. The clinical use of EA (Edecrin) as an anticancer drug is limited by its potent loop diuretic activity. In this study, we developed a non-diuretic 2-amino-2-deoxy-d-glucose conjugated EA (EAG) to target tumors cells via the highly expressed glucose transporter 1 (GLUT1). Cell survival assays revealed that EAG had little effect on normal cells, but was cytotoxic 3 to 4.5-fold greater than EA. Mechanistically, the EAG induced selective cell death in cancer cells by inhibiting GSTP1 and generating abundant reactive oxygen species. Furthermore, EAG induced p21(cip1) expression and a G2/M cell cycle block irrespective of the p53 gene status in tumor cells. These data encourage the development of new EA analogs.
[Mh] Termos MeSH primário: Antineoplásicos/farmacologia
Inibidores Enzimáticos/farmacologia
Ácido Etacrínico/farmacologia
Glucosamina/farmacologia
[Mh] Termos MeSH secundário: Antineoplásicos/síntese química
Antineoplásicos/química
Linhagem Celular Tumoral
Proliferação Celular/efeitos dos fármacos
Relação Dose-Resposta a Droga
Ensaios de Seleção de Medicamentos Antitumorais
Inibidores Enzimáticos/síntese química
Inibidores Enzimáticos/química
Ácido Etacrínico/síntese química
Glucosamina/análogos & derivados
Glucosamina/química
Glutationa S-Transferase pi/antagonistas & inibidores
Glutationa S-Transferase pi/metabolismo
Seres Humanos
Estrutura Molecular
Relação Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (Enzyme Inhibitors); EC 2.5.1.18 (GSTP1 protein, human); EC 2.5.1.18 (Glutathione S-Transferase pi); M5DP350VZV (Ethacrynic Acid); N08U5BOQ1K (Glucosamine)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171122
[Lr] Data última revisão:
171122
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160510
[St] Status:MEDLINE


  7 / 2138 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27113863
[Au] Autor:Kölln C; Reichl S
[Ad] Endereço:Institut für Pharmazeutische Technologie, Technische Universität Braunschweig, Braunschweig, Germany. Electronic address: c.koelln@tu-braunschweig.de.
[Ti] Título:Expression of glutathione transferases in corneal cell lines, corneal tissues and a human cornea construct.
[So] Source:Int J Pharm;506(1-2):371-81, 2016 Jun 15.
[Is] ISSN:1873-3476
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Glutathione transferase (GST) expression and activity were examined in a three-dimensional human cornea construct and were compared to those of excised animal corneas. The objective of this study was to characterize phase II enzyme expression in the cornea construct with respect to its utility as an alternative to animal cornea models. The expression of the GSTO1-1 and GSTP1-1 enzymes was investigated using immunofluorescence staining and western blotting. The level of total glutathione transferase activity was determined using 1-chloro-2,4- dinitrobenzene as the substrate. Furthermore, the levels of GSTO1-1 and GSTP1-1 activity were examined using S-(4-nitrophenacyl)glutathione and ethacrynic acid, respectively, as the specific substrates. The expression and activity levels of these enzymes were examined in the epithelium, stroma and endothelium, the three main cellular layers of the cornea. In summary, the investigated enzymes were detected at both the protein and functional levels in the cornea construct and the excised animal corneas. However, the enzymatic activity levels of the human cornea construct were lower than those of the animal corneas.
[Mh] Termos MeSH primário: Córnea/enzimologia
Epitélio Anterior/enzimologia
Glutationa S-Transferase pi/metabolismo
Glutationa Transferase/metabolismo
[Mh] Termos MeSH secundário: Animais
Western Blotting
Linhagem Celular
Ácido Etacrínico/metabolismo
Imunofluorescência
Glutationa/análogos & derivados
Glutationa/metabolismo
Seres Humanos
Coelhos
Especificidade da Espécie
Suínos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (S-(4-nitrophenacyl)glutathione); EC 2.5.1.18 (GSTO1 protein, human); EC 2.5.1.18 (GSTP1 protein, human); EC 2.5.1.18 (Glutathione S-Transferase pi); EC 2.5.1.18 (Glutathione Transferase); GAN16C9B8O (Glutathione); M5DP350VZV (Ethacrynic Acid)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170410
[Lr] Data última revisão:
170410
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160427
[St] Status:MEDLINE


  8 / 2138 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
PubMed Central Texto completo
Texto completo
[PMID]:26645909
[Au] Autor:Schulz M; Filary B; Kühn S; Colby T; Harzen A; Schmidt J; Sicker D; Hennig L; Hofmann D; Disko U; Anders N
[Ad] Endereço:a IMBIO Institut für Biotechnologie der Pflanzen, Universität Bonn , Karlrobert Kreiten Str. 13, 53115 Bonn , Germany.
[Ti] Título:Benzoxazolinone detoxification by N-Glucosylation: The multi-compartment-network of Zea mays L.
[So] Source:Plant Signal Behav;11(1):e1119962, 2016.
[Is] ISSN:1559-2324
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The major detoxification product in maize roots after 24 h benzoxazolin-2(3H)-one (BOA) exposure was identified as glucoside carbamate resulting from rearrangement of BOA-N-glucoside, but the pathway of N-glucosylation, enzymes involved and the site of synthesis were previously unknown. Assaying whole cell proteins revealed the necessity of H2O2 and Fe(2+) ions for glucoside carbamate production. Peroxidase produced BOA radicals are apparently formed within the extraplastic space of the young maize root. Radicals seem to be the preferred substrate for N-glucosylation, either by direct reaction with glucose or, more likely, the N-glucoside is released by glucanase/glucosidase catalyzed hydrolysis from cell wall components harboring fixed BOA. The processes are accompanied by alterations of cell wall polymers. Glucoside carbamate accumulation could be suppressed by the oxireductase inhibitor 2-bromo-4´-nitroacetophenone and by peroxidase inhibitor 2,3-butanedione. Alternatively, activated BOA molecules with an open heterocycle may be produced by microorganisms (e.g., endophyte Fusarium verticillioides) and channeled for enzymatic N-glucosylation. Experiments with transgenic Arabidopsis lines indicate a role of maize glucosyltransferase BX9 in BOA-N-glycosylation. Western blots with BX9 antibody demonstrate the presence of BX9 in the extraplastic space. Proteomic analyses verified a high BOA responsiveness of multiple peroxidases in the apoplast/cell wall. BOA incubations led to shifting, altered abundances and identities of the apoplast and cell wall located peroxidases, glucanases, glucosidases and glutathione transferases (GSTs). GSTs could function as glucoside carbamate transporters. The highly complex, compartment spanning and redox-regulated glucoside carbamate pathway seems to be mainly realized in Poaceae. In maize, carbamate production is independent from benzoxazinone synthesis.
[Mh] Termos MeSH primário: Benzoxazóis/metabolismo
Zea mays/metabolismo
[Mh] Termos MeSH secundário: Acetofenonas/farmacologia
Arabidopsis/efeitos dos fármacos
Arabidopsis/genética
Benzoxazóis/química
Benzoxazóis/farmacologia
Bioensaio
Western Blotting
Carbamatos/metabolismo
Parede Celular/efeitos dos fármacos
Parede Celular/metabolismo
Cromatografia Líquida de Alta Pressão
Citosol/efeitos dos fármacos
Citosol/metabolismo
Diacetil/farmacologia
Ácido Etacrínico/farmacologia
Fusarium/efeitos dos fármacos
Fusarium/fisiologia
Glucosídeos/metabolismo
Glutationa Transferase/metabolismo
Glicosilação/efeitos dos fármacos
Inativação Metabólica/efeitos dos fármacos
Peroxidases/metabolismo
Proteínas de Plantas/metabolismo
Raízes de Plantas/efeitos dos fármacos
Raízes de Plantas/metabolismo
Plantas Geneticamente Modificadas
Plântulas/efeitos dos fármacos
Plântulas/metabolismo
Zea mays/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Acetophenones); 0 (Benzoxazoles); 0 (Carbamates); 0 (Glucosides); 0 (Plant Proteins); 3X996Q809V (benzoxazolone); 99-81-0 (2-bromo-4'-nitroacetophenone); EC 1.11.1.- (Peroxidases); EC 2.5.1.18 (Glutathione Transferase); K324J5K4HM (Diacetyl); M5DP350VZV (Ethacrynic Acid)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151210
[St] Status:MEDLINE
[do] DOI:10.1080/15592324.2015.1119962


  9 / 2138 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:26605909
[Au] Autor:Jang JH; Lee HS; Oh SH; Park MH
[Ad] Endereço:a Department of Otorhinolaryngology , Kyungpook National University College of Medicine , Daegu , Korea ;
[Ti] Título:Efficacy of the cat deafening method: Co-administration of ethacrynic acid and kanamycin.
[So] Source:Acta Otolaryngol;136(3):289-92, 2016.
[Is] ISSN:1651-2251
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: This study was designed to determine if hearing status monitoring during intravenous infusion of EA reduces individual variability and to evaluate the correlation between EA dose and Bwt. MATERIALS AND METHODS: Twenty-five cats with the mean age of 24 ± 3.7 weeks (range = 20.6-28.3) and a mean weight of 3.21 ± 0.84 kg (range = 1.9-5.1) were administered a subcutaneous injection of KM (300 mg/kg) followed by an intravenous infusion of EA (1 mg/min). Click-evoked auditory brainstem responses (ABRs) were recorded to monitor hearing during the infusion. When ABR thresholds exceeded a 90 dB sound pressure level, the infusion of EA was terminated. Histopathology forapex, middle, and base sections of the cochlea were examined after 6 months. RESULTS: The dose of EA was optimized for deafening through simultaneous ABR measurements. Bwt was positively correlated with EA dose (mg) (p < 0.001, R(2) = 0.548), which was different from a study previously reported. Cochlear histopathology assessments revealed an absence of organ of Corti in the majority of cochleae. CONCLUSION: Co-administration of kanamycin (KM) and ethacrynic acid (EA) was an easy and effective method for deafening procedures in adult animals. Body weight (Bwt) was positively correlated with EA dose (mg) and an optimal EA dose can be calculated.
[Mh] Termos MeSH primário: Gatos
Surdez/induzido quimicamente
Modelos Animais de Doenças
[Mh] Termos MeSH secundário: Animais
Surdez/patologia
Ácido Etacrínico
Canamicina
Órgão Espiral/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; VALIDATION STUDIES
[Nm] Nome de substância:
59-01-8 (Kanamycin); M5DP350VZV (Ethacrynic Acid)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151126
[St] Status:MEDLINE
[do] DOI:10.3109/00016489.2015.1110751


  10 / 2138 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:26475825
[Au] Autor:Dou W; Wu JJ; Chen SC; Wei DD; Wang JJ
[Ad] Endereço:Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400715, P. R. China (douwei80@swu.edu.cn; 30356404@qq.com; 419093553@qq.com; weidandande@163.com; wangjinjun@swu.edu.cn).
[Ti] Título:Effects of Ethacrynic Acid Addition to Diet on Fitness and Development in the Psocid Liposcelis bostrychophila Badonnel.
[So] Source:Environ Entomol;45(1):252-7, 2016 Feb.
[Is] ISSN:1938-2936
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Fertility life table provides a comprehensive description of arthropod population dynamics by the estimation of parameters about arthropod population growth potential. It can also clarify the sublethal effects of chemicals on insects. Ethacrynic acid (EA), an inhibitor of glutathione S-transferases, is a diuretic compound that has been confirmed to modulate drug resistance in organisms. In this study, the effects of EA on growth and development of Liposcelis bostrychophila Badonnel were investigated in the laboratory to explore the potential possibilities of EA as an active agent to manage insecticide-resistant psocids. The treatment of psocids was obtained by feeding on the routine diet containing 3% EA for three successive generations, and psocids on routine diet served as control. The results indicated that EA possessed some negative effects on the life-table parameters of the psocid in F1 and F2 generations. The addition of EA to diet stunted psocids growth by lengthening development time and increasing mortality with a greater effect in the F2 generation. In the third generation of psocids on EA diet, it seemed there was a return to normal. Psocid fitness was influenced by addition of EA to standard diet. Using rm values, the fitness for EA diet in F1, F2, and F3 compared with the counterpart of routine diet was calculated as 0.80, 0.74, and 0.87, respectively.
[Mh] Termos MeSH primário: Ácido Etacrínico/farmacologia
Aptidão Genética/efeitos dos fármacos
Insetos/efeitos dos fármacos
[Mh] Termos MeSH secundário: Ração Animal/análise
Animais
Dieta
Fertilidade/efeitos dos fármacos
Insetos/genética
Insetos/crescimento & desenvolvimento
Tábuas de Vida
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
M5DP350VZV (Ethacrynic Acid)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151018
[St] Status:MEDLINE
[do] DOI:10.1093/ee/nvv157



página 1 de 214 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde