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[PMID]:28457955
[Au] Autor:Tapadiya A; Vasanthan N
[Ad] Endereço:Department of Chemistry and Biochemistry, Long Island University, One University Plaza, Brooklyn, NY 11201, United States.
[Ti] Título:Crystallization and alkaline hydrolysis of poly(3- hydroxybutyrate) films probed by thermal analysis and infrared spectroscopy.
[So] Source:Int J Biol Macromol;102:1130-1137, 2017 Sep.
[Is] ISSN:1879-0003
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Poly(3-hydroxybutyrate) (PHB) is a microbially synthesized polymer, which is often purified by alkaline treatment. The effect of microstructure on alkaline hydrolysis has been studied by varying concentration of base and the temperature. The morphologies of PHB films before and after degradation were evaluated using DSC and FTIR spectroscopy. The hydrolytic degradation study by weight loss measurement revealed that the crystallinity of PHB greatly decreased the hydrolytic ability of PHB. The crystallization of PHB and the effect of base on hydrolysis was investigated by time dependent FTIR spectroscopy. The normalized absorbance of 3010cm and 1183cm were used to characterize the crystalline and the amorphous phases of PHB. FTIR spectroscopy reveal that the extent of hydrolysis decreased with increasing crystallinity. The crotonic acid was detected as a major product after hydrolysis, confirmed by UV/Visible and proton NMR spectroscopy. The normalized absorbance of the crystalline band at 3010cm band remained constant, suggesting that there is no significant change in crystallinity with degradation. The normalized amorphous band at 1183cm showed a decrease in absorbance ratio, suggesting degradation of the amorphous phase. Our data suggests that alkaline hydrolysis depends on concentration of base and the crystallinity of PHB.
[Mh] Termos MeSH primário: Ácido 3-Hidroxibutírico/química
Temperatura Ambiente
[Mh] Termos MeSH secundário: Cristalização
Concentração de Íons de Hidrogênio
Hidrólise
Espectroscopia de Infravermelho com Transformada de Fourier
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
TZP1275679 (3-Hydroxybutyric Acid)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180306
[Lr] Data última revisão:
180306
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170502
[St] Status:MEDLINE


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[PMID]:29311546
[Au] Autor:Yang JE; Park SJ; Kim WJ; Kim HJ; Kim BJ; Lee H; Shin J; Lee SY
[Ad] Endereço:Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 Plus Program), Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, 34141, Repu
[Ti] Título:One-step fermentative production of aromatic polyesters from glucose by metabolically engineered Escherichia coli strains.
[So] Source:Nat Commun;9(1):79, 2018 01 08.
[Is] ISSN:2041-1723
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Aromatic polyesters are widely used plastics currently produced from petroleum. Here we engineer Escherichia coli strains for the production of aromatic polyesters from glucose by one-step fermentation. When the Clostridium difficile isocaprenoyl-CoA:2-hydroxyisocaproate CoA-transferase (HadA) and evolved polyhydroxyalkanoate (PHA) synthase genes are overexpressed in a D-phenyllactate-producing strain, poly(52.3 mol% 3-hydroxybutyrate (3HB)-co-47.7 mol% D-phenyllactate) can be produced from glucose and sodium 3HB. Also, various poly(3HB-co-D-phenyllactate) polymers having 11.0, 15.8, 20.0, 70.8, and 84.5 mol% of D-phenyllactate are produced from glucose as a sole carbon source by additional expression of Ralstonia eutropha ß-ketothiolase (phaA) and reductase (phaB) genes. Fed-batch culture of this engineered strain produces 13.9 g l of poly(61.9 mol% 3HB-co-38.1 mol% D-phenyllactate). Furthermore, different aromatic polyesters containing D-mandelate and D-3-hydroxy-3-phenylpropionate are produced from glucose when feeding the corresponding monomers. The engineered bacterial system will be useful for one-step fermentative production of aromatic polyesters from renewable resources.
[Mh] Termos MeSH primário: Escherichia coli/metabolismo
Fermentação
Glucose/metabolismo
Engenharia Metabólica/métodos
Poliésteres/metabolismo
[Mh] Termos MeSH secundário: Ácido 3-Hidroxibutírico/metabolismo
Acetil-CoA C-Aciltransferase/genética
Acetil-CoA C-Aciltransferase/metabolismo
Aciltransferases/genética
Aciltransferases/metabolismo
Proteínas de Bactérias/genética
Proteínas de Bactérias/metabolismo
Clostridium difficile/enzimologia
Clostridium difficile/genética
Coenzima A-Transferases/genética
Coenzima A-Transferases/metabolismo
Cupriavidus necator/enzimologia
Cupriavidus necator/genética
Escherichia coli/genética
Lactatos/metabolismo
Oxirredutases/genética
Oxirredutases/metabolismo
Polietilenotereftalatos/metabolismo
Polímeros/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Lactates); 0 (Polyesters); 0 (Polyethylene Terephthalates); 0 (Polymers); 156-05-8 (3-phenyllactic acid); EC 1.- (Oxidoreductases); EC 2.3.- (Acyltransferases); EC 2.3.1.- (poly(3-hydroxyalkanoic acid) synthase); EC 2.3.1.16 (Acetyl-CoA C-Acyltransferase); EC 2.8.3.- (Coenzyme A-Transferases); IY9XDZ35W2 (Glucose); TZP1275679 (3-Hydroxybutyric Acid)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180110
[St] Status:MEDLINE
[do] DOI:10.1038/s41467-017-02498-w


  3 / 3401 MEDLINE  
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[PMID]:28870733
[Au] Autor:Stenger B; Gerber A; Bernhardt R; Hannemann F
[Ad] Endereço:Institute of Biochemistry, Saarland University, Saarbrücken, Germany.
[Ti] Título:Functionalized poly(3-hydroxybutyric acid) bodies as new in vitro biocatalysts.
[So] Source:Biochim Biophys Acta;1866(1):52-59, 2018 01.
[Is] ISSN:0006-3002
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Cytochromes P450 play a key role in the drug and steroid metabolism in the human body. This leads to a high interest in this class of proteins. Mammalian cytochromes P450 are rather delicate. Due to their localization in the mitochondrial or microsomal membrane, they tend to aggregate during expression and purification and to convert to an inactive form so that they have to be purified and stored in complex buffers. The complex buffers and low storage temperatures, however, limit the feasibility of fast, automated screening of the corresponding cytochrome P450-effector interactions, which are necessary to study substrate-protein and inhibitor-protein interactions. Here, we present the production and isolation of functionalized poly(3-hydroxybutyrate) granules (PHB bodies) from Bacillus megaterium MS941 strain. In contrast to the expression in Escherichia coli, where mammalian cytochromes P450 are associated to the cell membrane, when CYP11A1 is heterologously expressed in Bacillus megaterium, it is located on the PHB bodies. The surface of these particles provides a matrix for immobilization and stabilization of the CYP11A1 during the storage of the protein and substrate conversion. It was demonstrated that the PHB polymer basis is inert concerning the performed conversion. Immobilization of the CYP11A1 onto the PHB bodies allows freeze-drying of the complex without significant decrease of the CYP11A1 activity. This is the first lyophilization of a mammalian cytochrome P450, which allows storage over more than 18days at 4°C instead of storage at -80°C. In addition, we were able to immobilize the cytochrome P450 on the PHB bodies in vitro. In this case the expression of the protein is separated from the production of the immobilization matrix, which widens the application of this method. This article is part of a Special Issue entitled: Cytochrome P450 biodiversity and biotechnology, edited by Erika Plettner, Gianfranco Gilardi, Luet Wong, Vlada Urlacher, Jared Goldstone.
[Mh] Termos MeSH primário: Ácido 3-Hidroxibutírico/química
Bacillus megaterium/genética
Biotecnologia/métodos
Enzima de Clivagem da Cadeia Lateral do Colesterol/química
Proteínas Imobilizadas/biossíntese
Proteínas Mitocondriais/biossíntese
[Mh] Termos MeSH secundário: Ácido 3-Hidroxibutírico/biossíntese
Animais
Bacillus megaterium/enzimologia
Biocatálise
Bovinos
Colesterol/química
Colesterol/metabolismo
Enzima de Clivagem da Cadeia Lateral do Colesterol/genética
Enzima de Clivagem da Cadeia Lateral do Colesterol/metabolismo
Grânulos Citoplasmáticos/química
Liofilização
Expressão Gênica
Proteínas Imobilizadas/química
Proteínas Imobilizadas/genética
Proteínas Mitocondriais/química
Proteínas Mitocondriais/genética
Pregnenolona/biossíntese
Pregnenolona/química
Refrigeração
Transgenes
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Immobilized Proteins); 0 (Mitochondrial Proteins); 73R90F7MQ8 (Pregnenolone); 97C5T2UQ7J (Cholesterol); EC 1.14.15.6 (Cholesterol Side-Chain Cleavage Enzyme); TZP1275679 (3-Hydroxybutyric Acid)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180208
[Lr] Data última revisão:
180208
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170906
[St] Status:MEDLINE


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[PMID]:28456470
[Au] Autor:Arikawa H; Sato S; Fujiki T; Matsumoto K
[Ad] Endereço:GP Group, Corporate R&D Planning and Administration Division, Kaneka Corporation, 1-8 Miyamae-Cho, Takasago-Cho, Takasago, Hyogo 676-8688, Japan. Electronic address: Hisashi.Arikawa@kaneka.co.jp.
[Ti] Título:Simple and rapid method for isolation and quantitation of polyhydroxyalkanoate by SDS-sonication treatment.
[So] Source:J Biosci Bioeng;124(2):250-254, 2017 Aug.
[Is] ISSN:1347-4421
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:We developed a new method for isolation and quantitation of polyhydroxyalkanoate (PHA) from culture broth. In this method, the cells were sonicated in sodium dodecyl sulfate (SDS) solution and centrifuged to recover PHA. The recovered PHA was rinsed with deionized water and ethanol, and then weighed after drying. Hazardous chemicals such as chloroform, methanol, and sulfuric acid were not used, and no expensive analytical instruments were needed. We applied this method to Cupriavidus necator culture broths that included various amounts of poly(3-hydroxybutyrate) (PHB) or poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) from flasks and jar fermentors. The quantitation by this method was practical for use with a wide range of production amounts and PHA monomer compositions compared to the conventional whole-cell methanolysis method with gas chromatographic analysis, and besides, the recovered PHAs were adequately pure (≥96% purity). Therefore, this new method would be valuable not only for quantitation of PHA but also for preparation of samples to characterize their mechanical properties.
[Mh] Termos MeSH primário: Cupriavidus necator/química
Poli-Hidroxialcanoatos/análise
Poli-Hidroxialcanoatos/isolamento & purificação
Sonicação/métodos
[Mh] Termos MeSH secundário: Ácido 3-Hidroxibutírico
Caproatos
Cupriavidus necator/metabolismo
Poli-Hidroxialcanoatos/metabolismo
Dodecilsulfato de Sódio/química
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Caproates); 0 (Polyhydroxyalkanoates); 0 (poly(3-hydroxybutyrate-co-3-hydroxyhexanoate)); 368GB5141J (Sodium Dodecyl Sulfate); TZP1275679 (3-Hydroxybutyric Acid)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180116
[Lr] Data última revisão:
180116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170501
[St] Status:MEDLINE


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[PMID]:29173326
[Au] Autor:Futatani T; Shimao A; Ina S; Higashiyama H; Fujita S; Ueno K; Igarashi N; Hatasaki K
[Ad] Endereço:Department of Pediatrics, Toyama Prefectural Central Hospital, Toyama, Japan. Electronic address: futatani@tch.pref.toyama.jp.
[Ti] Título:Capillary Blood Ketone Levels as an Indicator of Inadequate Breast Milk Intake in the Early Neonatal Period.
[So] Source:J Pediatr;191:76-81, 2017 Dec.
[Is] ISSN:1097-6833
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: To determine the utility of capillary blood ketone levels as an indicator of inadequate intake of breast milk in the early postnatal period. STUDY DESIGN: Levels of capillary blood beta-hydroxybutyrate (ßOHB), the main ketone body in the blood, were measured with a bedside ketone meter in 585 full-term neonates aged 48-95 hours who were breastfed exclusively. Relationships between weight-loss percentage, blood sodium, glucose, pH, partial pressure of carbon dioxide, base-deficit levels, and ßOHB levels were investigated. The diagnostic accuracy of ßOHB for predicting excessive weight loss (weight loss ≥10% of birth weight) and hypernatremic dehydration (blood sodium level ≥150 mEq/L) was determined. RESULTS: ßOHB levels were correlated positively with weight-loss percentage and blood sodium levels and were correlated negatively with blood glucose levels. The diagnostic accuracy of ßOHB was 0.846 (optimal cut off, 1.55 mmol/L; sensitivity, 80.9%, specificity, 74.0%) for predicting excessive weight loss and 0.868 (optimal cut off, 1.85 mmol/L; sensitivity, 94.3%; specificity, 69.9%) for predicting hypernatremic dehydration according to the area under the receiver operating characteristic curve. Multiple logistic analysis revealed that ßOHB and weight loss percentage were the only independent predictors of hypernatremic dehydration. Increases in ßOHB levels also were associated with worsening metabolic acidosis and hypocapnia. CONCLUSION: High ßOHB levels were associated with inadequate intake of breast milk in the early postnatal period. The use of bedside capillary blood ketone levels may be clinically useful as an indicator of dehydration, energy depletion, and acid-base imbalance in breastfeeding infants in the early postnatal period.
[Mh] Termos MeSH primário: Ácido 3-Hidroxibutírico/sangue
Desequilíbrio Ácido-Base/diagnóstico
Aleitamento Materno
Desidratação/diagnóstico
Desnutrição/diagnóstico
[Mh] Termos MeSH secundário: Desequilíbrio Ácido-Base/sangue
Desequilíbrio Ácido-Base/etiologia
Biomarcadores/sangue
Capilares
Desidratação/sangue
Desidratação/etiologia
Feminino
Seres Humanos
Cuidado do Lactente
Recém-Nascido
Modelos Logísticos
Masculino
Desnutrição/sangue
Desnutrição/etiologia
Testes Imediatos
Sensibilidade e Especificidade
Perda de Peso
[Pt] Tipo de publicação:CLINICAL TRIAL; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); TZP1275679 (3-Hydroxybutyric Acid)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171211
[Lr] Data última revisão:
171211
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:171128
[St] Status:MEDLINE


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[PMID]:29036204
[Au] Autor:Han P; Huang Y; Xie Y; Yang W; Wang Y; Xiang W; Hylands PJ; Legido-Quigley C
[Ad] Endereço:Institute of Pharmaceutical Science, Faculty of Life Sciences & Medicine, King's College London, London, United Kingdom.
[Ti] Título:Metabolic phenotyping in the mouse model of urinary tract infection shows that 3-hydroxybutyrate in plasma is associated with infection.
[So] Source:PLoS One;12(10):e0186497, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Urinary tract infection is one of the most common bacterial infections worldwide. Current diagnosis of urinary tract infection chiefly relies on its clinical presentation, urine dipstick tests and urine culture. Small molecules found in bio-fluids related with both infection and recovery would facilitate diagnosis and management of UTI. Mass spectrometry-based fingerprinting of plasma and urine at 3 time points, pre-infection (t = -24h), infection (t = 24h) and post 3-day treatment (t = 112h), were acquired in the following four groups: mice which were healthy, infected but not treated, infected and treated with ciprofloxacin, and infected and treated with Relinqing® granules (n = 6 per group). A metabolomics workflow including multivariate analysis and ROC regression was employed to select metabolic features that correlated with UTI and its treatment. Circa 4,000 molecular features were acquired for each sample. The small acid 3-hydroxybutyrate in plasma was found to be differentiated for urinary tract infection, with an area under the curve = 0.97 (95% confidence interval: 0.93-1.00, accuracy = 0.91, sensitivity = 0.92 and specificity = 0.91). The level of 3-hydroxybutyrate in plasma was depleted after infection with a fold change of -22 (q < 0.0001). Correlation between plasma 3-hydroxybutyrate and urine bacterial number in all groups and time points was r = -0.753 (p < 0.0001). The findings show that 3-hydroxybutyrate is depleted in blood and strongly associated with UTI at both infection and post-treatment stage in a UTI mouse model. Further work is envisaged to assess the clinical potential of blood tests to assist with UTI management.
[Mh] Termos MeSH primário: Ácido 3-Hidroxibutírico/sangue
Metabolômica
Fenótipo
Infecções Urinárias/sangue
[Mh] Termos MeSH secundário: Ácido 3-Hidroxibutírico/urina
Animais
Modelos Animais de Doenças
Cães
Feminino
Camundongos
Fatores de Tempo
Infecções Urinárias/urina
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
TZP1275679 (3-Hydroxybutyric Acid)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171106
[Lr] Data última revisão:
171106
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171017
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0186497


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[PMID]:28826372
[Au] Autor:Newman JC; Verdin E
[Ad] Endereço:Buck Institute for Research on Aging, Novato, California 94945; email: newman@ucsf.edu , everdin@buckinstitute.org.
[Ti] Título:ß-Hydroxybutyrate: A Signaling Metabolite.
[So] Source:Annu Rev Nutr;37:51-76, 2017 Aug 21.
[Is] ISSN:1545-4312
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Various mechanisms in the mammalian body provide resilience against food deprivation and dietary stress. The ketone body ß-hydroxybutyrate (BHB) is synthesized in the liver from fatty acids and represents an essential carrier of energy from the liver to peripheral tissues when the supply of glucose is too low for the body's energetic needs, such as during periods of prolonged exercise, starvation, or absence of dietary carbohydrates. In addition to its activity as an energetic metabolite, BHB is increasingly understood to have cellular signaling functions. These signaling functions of BHB broadly link the outside environment to epigenetic gene regulation and cellular function, and their actions may be relevant to a variety of human diseases as well as human aging.
[Mh] Termos MeSH primário: Ácido 3-Hidroxibutírico/metabolismo
Transdução de Sinais
[Mh] Termos MeSH secundário: Envelhecimento
Animais
Epigênese Genética
Seres Humanos
Fígado/metabolismo
Inanição
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
TZP1275679 (3-Hydroxybutyric Acid)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171002
[Lr] Data última revisão:
171002
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170823
[St] Status:MEDLINE
[do] DOI:10.1146/annurev-nutr-071816-064916


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[PMID]:28803103
[Au] Autor:Chen X; Yin J; Ye J; Zhang H; Che X; Ma Y; Li M; Wu LP; Chen GQ
[Ad] Endereço:School of Life Sciences, Tsinghua University, Beijing 100084, China.
[Ti] Título:Engineering Halomonas bluephagenesis TD01 for non-sterile production of poly(3-hydroxybutyrate-co-4-hydroxybutyrate).
[So] Source:Bioresour Technol;244(Pt 1):534-541, 2017 Nov.
[Is] ISSN:1873-2976
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Poly(3-hydroxybutyrate-co-4-hydroxybutyrate), short as P(3HB-co-4HB), was successfully produced by engineered Halomonas bluephagenesis TD01 grown in glucose and γ-butyrolactone under open non-sterile conditions. Gene orfZ encoding 4HB-CoA transferase of Clostridium kluyveri was integrated into the genome to achieve P(3HB-co-4HB) accumulation comparable to that of strains encoding orfZ on plasmids. Fed-batch cultivations conducted in 1-L and 7-L fermentors, respectively, resulted in over 70g/L cell dry weight (CDW) containing 63% P(3HB-co-12mol% 4HB) after 48h under non-sterile conditions. The processes were further scaled up in a 1000-L pilot fermentor to reach 83g/L CDW containing 61% P(3HB-co-16mol% 4HB) in 48h, with a productivity of 1.04g/L/h, again, under non-sterile conditions. The elastic P(3HB-co-16mol% 4HB) shows an elongation at break of 1022±43%. Results demonstrate that the engineered Halomonas bluephagenesis TD01 is a suitable industrial strain for large scale production under open non-sterile conditions.
[Mh] Termos MeSH primário: Halomonas
Hidroxibutiratos
Poliésteres
[Mh] Termos MeSH secundário: Ácido 3-Hidroxibutírico
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Hydroxybutyrates); 0 (Polyesters); 117068-64-1 (poly(3-hydroxybutyrate-co-4-hydroxybutyrate)); TZP1275679 (3-Hydroxybutyric Acid)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171106
[Lr] Data última revisão:
171106
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170814
[St] Status:MEDLINE


  9 / 3401 MEDLINE  
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[PMID]:28715817
[Au] Autor:Chen G; Fu S; Feng W; Huang B; Xu S; Wang W; Liu J
[Ti] Título:AMP010014A09 in Sus Scrofa Encodes an Analog of G Protein-Coupled Receptor 109A, Which Mediates the Anti-Inflammatory Effects of Beta-Hydroxybutyric Acid.
[So] Source:Cell Physiol Biochem;42(4):1420-1430, 2017.
[Is] ISSN:1421-9778
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Hydroxy-carboxylic acid receptor 2 (HCA2, also called GPR109A) belongs to the G protein-coupled receptor (GPCR) family and is found in humans, rats, mice, hamsters and guinea pigs, but there are almost no reports of this protein in other species. In this investigation, we speculated that AMP010014A09 (AMP+) is a homologue of GPR109A in swine. METHODS: To test this hypothesis, the following experiments were designed: monocytes isolated from the peripheral blood of swine were treated with LPS after pretreating with or without ß-hydroxybutyric acid (BHBA), and the levels of pro-inflammatory cytokines and inflammatory proteins were assessed. cAMP levels induced by Forskolin in swine testicular (ST) and IPEC-J2 cells were detected with or without BHBA treatment and following silencing or stable transfection of the AMP+ gene. RESULTS: AMP+ in swine exhibited a high level of homology with HM74A in humans and PUMA-G in mice. BHBA inhibited the LPS-induced secretion of the pro-inflammatory cytokines TNF-α, IL-6 and IL-1ß and the inflammatory protein COX-2 in monocytes of swine. BHBA suppressed the Forskolin-induced cAMP level increase in ST cells, but failed to inhibit the accumulation of cAMP after the AMP+ gene was silenced with shRNA by transfecting cells with the pGPU6-GFP-Neo-AMP+-sus-392 plasmid. BHBA had no effect on cAMP levels in IPEC-J2 cells, but significantly inhibited the increase in cAMP induced by Forskolin treatment following transfection of the AMP+ gene into IPEC-J2 cells by a lentivirus vector. CONCLUSION: Our results indicated that AMP+ encodes a G protein-coupled receptor in Sus scrofa that inhibits cAMP levels and mediates anti-inflammatory effects in swine monocytes.
[Mh] Termos MeSH primário: Ácido 3-Hidroxibutírico/farmacologia
Anti-Inflamatórios não Esteroides/farmacologia
AMP Cíclico/imunologia
Monócitos/efeitos dos fármacos
Receptores Acoplados a Proteínas-G/imunologia
Receptores Nicotínicos/imunologia
[Mh] Termos MeSH secundário: Animais
Linhagem Celular
Colforsina/antagonistas & inibidores
Colforsina/farmacologia
AMP Cíclico/antagonistas & inibidores
AMP Cíclico/metabolismo
Células Epiteliais/citologia
Células Epiteliais/efeitos dos fármacos
Células Epiteliais/imunologia
Expressão Gênica
Intestinos/citologia
Intestinos/efeitos dos fármacos
Intestinos/imunologia
Lipopolissacarídeos/antagonistas & inibidores
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[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents, Non-Steroidal); 0 (HCAR2 protein, human); 0 (Hcar2 protein, mouse); 0 (Hcar2 protein, rat); 0 (Lipopolysaccharides); 0 (Protein Isoforms); 0 (RNA, Small Interfering); 0 (Receptors, G-Protein-Coupled); 0 (Receptors, Nicotinic); 1F7A44V6OU (Colforsin); E0399OZS9N (Cyclic AMP); TZP1275679 (3-Hydroxybutyric Acid)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171026
[Lr] Data última revisão:
171026
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170718
[St] Status:MEDLINE
[do] DOI:10.1159/000479206


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[PMID]:28692918
[Au] Autor:Gahlawat G; Soni SK
[Ad] Endereço:Department of Microbiology, Panjab University, Chandigarh 160014, India. Electronic address: geeta.gahlawat@gmail.com.
[Ti] Título:Valorization of waste glycerol for the production of poly (3-hydroxybutyrate) and poly (3-hydroxybutyrate-co-3-hydroxyvalerate) copolymer by Cupriavidus necator and extraction in a sustainable manner.
[So] Source:Bioresour Technol;243:492-501, 2017 Nov.
[Is] ISSN:1873-2976
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Glycerol is a by-product of many industrial processes and huge amounts of it are generated in the form of waste, thereby necessitating a search for the method of its disposal. An interesting solution is the valorization of crude glycerol into value added product such as polyhydroxyalkanoates (PHAs). The feasibility of producing PHAs by Cupriavidus necator was evaluated using crude glycerol (WG). Various cultivation strategies were designed for the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) copolymer by adding different organic acids as precursors at different concentrations levels. Batch cultivation of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) production showed accumulation of 6.76g/L biomass containing 4.84g/L copolymer on WG with a maximum 3-hydroxyvalerate content of 24.6mol%. PHAs extraction using a non-toxic and recyclable solvent, 1,2 propylene carbonate, showed the highest recovery yield (90%) and purity (93%) at 120°C temperature and 30min incubation. This is the first report on jatropha based glycerol valorization for poly(3-hydroxybutyrate-co-3-hydroxyvalerate) production coupled with extraction using non-toxic solvent.
[Mh] Termos MeSH primário: Ácido 3-Hidroxibutírico
Cupriavidus necator
[Mh] Termos MeSH secundário: Glicerol
Ácidos Pentanoicos
Poliésteres
Eliminação de Resíduos Líquidos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Pentanoic Acids); 0 (Polyesters); 10237-77-1 (beta-hydroxyvaleric acid); PDC6A3C0OX (Glycerol); TZP1275679 (3-Hydroxybutyric Acid)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171023
[Lr] Data última revisão:
171023
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170711
[St] Status:MEDLINE



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