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[PMID]:28511111
[Au] Autor:Guijarro B; Larena I; Melgarejo P; De Cal A
[Ad] Endereço:Department of Plant Protection, Phytopathology Fungi Unit, National Institute for Agriculture and Food Research, INIA, Madrid, Spain.
[Ti] Título:Adaptive conditions and safety of the application of Penicillium frequentans as a biocontrol agent on stone fruit.
[So] Source:Int J Food Microbiol;254:25-35, 2017 Aug 02.
[Is] ISSN:1879-3460
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Penicillium frequentans (Pf909) reduces brown rot caused by Monilinia spp. in stone fruit. The registration of a microbial biocontrol agent in Europe requires information on the risks and safety of a biological product. This study focused on the impact of the physical environment on Pf909 survival and growth, Pf909 mycotoxin production on fruit surface, and the Pf909 resistance to commercial antifungal compounds used in animal and human medicine. The effect of temperature (4 to 37°C), water activity (0.999 to 0.900), pH (3 to 11), light intensity (0, 90 and 180lm) and photoperiod (0/24, 12/12, 16/8, 24/0; light/dark) on mycelial growth and sporulation of Pf909 were monitored for 10days in vitro on culture medium. Antifungal activity of antifungal compounds on mycelial growth of Pf909 was also measured by a quantitative micro spectrophotometric assay after 72h of incubation. The presence or absence of four non-volatile mycotoxins (patulin, penicillic acid, ochratoxin A and citrinin) on nectarine surfaces treated with Pf909 was determined by HPLC. Growth rate was significantly influenced by water activity, temperature and light exposure conditions. Pf909 showed maximum growth and sporulation at 22°C and 25°C, in wet conditions (0.999 water activity), with a pH5.6 to 9, and in darkness or a short light photoperiod. Our results showed all antifungal compounds used reduced significantly Pf909 mycelial growth at tested commercial doses. HPLC data analysis showed that 7days after biofungicide (Pf909) application there were no mycotoxin products on the surface of nectarine. Finally, no phylogenetic relationship has been shown among Pf909 and other species of Penicillium that produce mycotoxins. In conclusion, from an ecological point of view, Pf909 would establish and survive actively over a broad range of climatic conditions. The probability of risks to human and animal health is considered very low.
[Mh] Termos MeSH primário: Ascomicetos/crescimento & desenvolvimento
Agentes de Controle Biológico/efeitos adversos
Agentes de Controle Biológico/farmacologia
Inocuidade dos Alimentos/métodos
Frutas/microbiologia
Micotoxinas/isolamento & purificação
Penicillium/metabolismo
Prunus persica/microbiologia
[Mh] Termos MeSH secundário: Animais
Citrinina/isolamento & purificação
Europa (Continente)
Micélio/crescimento & desenvolvimento
Ocratoxinas/isolamento & purificação
Patulina/isolamento & purificação
Ácido Penicílico/isolamento & purificação
Filogenia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biological Control Agents); 0 (Mycotoxins); 0 (Ochratoxins); 1779SX6LUY (ochratoxin A); 3S697X6SNZ (Citrinin); 95X2BV4W8R (Patulin); ONL14K3AFD (Penicillic Acid)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170517
[St] Status:MEDLINE


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[PMID]:27105128
[Au] Autor:Nguyen HT; Yu NH; Jeon SJ; Lee HW; Bae CH; Yeo JH; Lee HB; Kim IS; Park HW; Kim JC
[Ad] Endereço:Department of Agricultural Chemistry, Institute of Environmentally Friendly Agriculture, College of Agriculture and Life Sciences, Chonnam National University, Gwangju, Korea.
[Ti] Título:Antibacterial activities of penicillic acid isolated from Aspergillus persii against various plant pathogenic bacteria.
[So] Source:Lett Appl Microbiol;62(6):488-93, 2016 Jun.
[Is] ISSN:1472-765X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:UNLABELLED: The emergence of pathogenic bacterial strains resistant to agrochemicals and the increasing demand for organic foods have led to the discovery of new antibacterial metabolites that can be used either directly or as a lead molecule for development of synthetic bactericides. During the screening of antibacterial fungal cultures, we found that one fungal strain, Aspergillus persii EML-HPB1-11, showed strong in vitro antibacterial activity against Xanthomonas arboricola pv. pruni (Xap) with a minimum inhibitory concentration (MIC) of 10% of fermentation broth filtrate. The active compound was identified as penicillic acid (PA: 3-methoxy-5-methyl-4-oxo-2,5-hexadienoic acid) by mass and NMR spectroscopy. The in vitro antibacterial activity of PA was tested against 12 phytopathogenic bacteria. All of the bacterial pathogens tested were highly inhibited by PA with MIC values of 12·3-111·1 µg ml(-1) . It also effectively suppressed the development of bacterial spot disease in detached peach leaves, showing control values of 82·4 and 94·1% at concentrations of 111·1 and 333·3 µg ml(-1) respectively. This is the first report on the production of PA by A. persii. This study suggests that PA can be used as a lead molecule for development of synthetic bactericides for control of various plant diseases. SIGNIFICANCE AND IMPACT OF THE STUDY: Penicillic acid (PA) produced by the seed-borne fungus Aspergillus persii EML-HPB1-11 showed antibacterial activity against various plant pathogenic bacteria. The compound effectively inhibited the growth of 12 plant pathogenic bacteria and successfully controlled bacterial spot disease on peach leaf. These results suggest that PA can be used as a lead molecule for development of synthetic agrochemicals to control plant bacterial diseases.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Aspergillus/metabolismo
Agentes de Controle Biológico/farmacologia
Ácido Penicílico/farmacologia
Xanthomonas/efeitos dos fármacos
[Mh] Termos MeSH secundário: Testes de Sensibilidade Microbiana
Doenças das Plantas/microbiologia
Folhas de Planta/microbiologia
Plantas/microbiologia
Sementes/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Biological Control Agents); ONL14K3AFD (Penicillic Acid)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160423
[St] Status:MEDLINE
[do] DOI:10.1111/lam.12578


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[PMID]:25712393
[Au] Autor:Nonaka K; Chiba T; Suga T; Asami Y; Iwatsuki M; Masuma R; Omura S; Shiomi K
[Ad] Endereço:Kitasato Institute for Life Sciences, Kitasato University, Tokyo, Japan.
[Ti] Título:Coculnol, a new penicillic acid produced by a coculture of Fusarium solani FKI-6853 and Talaromyces sp. FKA-65.
[So] Source:J Antibiot (Tokyo);68(8):530-2, 2015 Aug.
[Is] ISSN:0021-8820
[Cp] País de publicação:Japan
[La] Idioma:eng
[Mh] Termos MeSH primário: Antibacterianos/metabolismo
Fusarium/crescimento & desenvolvimento
Fusarium/metabolismo
Ácido Penicílico/análogos & derivados
Ácido Penicílico/metabolismo
Talaromyces/crescimento & desenvolvimento
Talaromyces/metabolismo
[Mh] Termos MeSH secundário: Antibacterianos/química
Antibacterianos/isolamento & purificação
Cromatografia Líquida de Alta Pressão
DNA Fúngico/química
DNA Fúngico/genética
Fusarium/classificação
Fusarium/genética
Seres Humanos
Espectroscopia de Ressonância Magnética
Modelos Moleculares
Dados de Sequência Molecular
Estrutura Molecular
Ácido Penicílico/química
Ácido Penicílico/isolamento & purificação
Análise de Sequência de DNA
Talaromyces/classificação
Talaromyces/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (DNA, Fungal); ONL14K3AFD (Penicillic Acid)
[Em] Mês de entrada:1511
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150226
[St] Status:MEDLINE
[do] DOI:10.1038/ja.2015.15


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[PMID]:24166155
[Au] Autor:Liaqat I; Bachmann RT; Edyvean RG
[Ad] Endereço:Department of Zoology, Government College University, Lahore, Pakistan, iramliaq@hotmail.com.
[Ti] Título:Type 2 quorum sensing monitoring, inhibition and biofilm formation in marine microrganisms.
[So] Source:Curr Microbiol;68(3):342-51, 2014 Mar.
[Is] ISSN:1432-0991
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The quorum sensing (QS) dependent behaviour of micro-organisms, in particular expression of virulence genes, biofilm formation and dispersal, have provided impetus for investigating practical approaches to interfere with microbial QS. This study tests Halomonas pacifica and Marinobacter hydrocarbonoclasticus, two halophilic marine micro-organism, for their AI-2 dependent QS signalling and the effect of two well-known quorum-sensing inhibitors (QSIs), patulin and penicillic acid, on biofilm formation. We report, for the first time, the successful amplification of a putative luxS gene in H. pacifica using degenerated primers and AI-2 dependent QS as well as inhibition using QSIs. Penicillic acid had a strong inhibitory effect on AI-2 induction of H. pacifica at non-growth inhibitory concentrations, while patulin has an adverse effect only at the highest concentration (25 µM). QSIs effect on biofilm forming capability was isolate specific, with maximum inhibition at 25 µM of patulin in H. pacifica. In M. hydrocarbonoclasticus, no adverse effects were noted at any tested concentration of either QSIs. Detection of bioluminescence and the presence of a putative luxS gene provide biochemical and genetic evidence for the production of a signalling molecule(s) which is the essential first step in characterizing H. pacifica QS. This study highlights the importance of AI-2 dependent QS in a marine setting, not previously reported. It further suggests that QSI compounds must be selected in the specific system in which they are to function, and they cannot easily be transferred from one QS system to another.
[Mh] Termos MeSH primário: Organismos Aquáticos/fisiologia
Biofilmes/crescimento & desenvolvimento
Halomonas/fisiologia
Marinobacter/fisiologia
Percepção de Quorum
[Mh] Termos MeSH secundário: Organismos Aquáticos/efeitos dos fármacos
Biofilmes/efeitos dos fármacos
Halomonas/efeitos dos fármacos
Homosserina/análogos & derivados
Homosserina/metabolismo
Lactonas/metabolismo
Marinobacter/efeitos dos fármacos
Patulina/metabolismo
Ácido Penicílico/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Lactones); 0 (N-octanoylhomoserine lactone); 6KA95X0IVO (Homoserine); 95X2BV4W8R (Patulin); ONL14K3AFD (Penicillic Acid)
[Em] Mês de entrada:1409
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:131030
[St] Status:MEDLINE
[do] DOI:10.1007/s00284-013-0484-5


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[PMID]:23360521
[Au] Autor:Vansteelandt M; Blanchet E; Egorov M; Petit F; Toupet L; Bondon A; Monteau F; Le Bizec B; Thomas OP; Pouchus YF; Le Bot R; Grovel O
[Ad] Endereço:LUNAM Université, Université de Nantes, Nantes, France.
[Ti] Título:Ligerin, an antiproliferative chlorinated sesquiterpenoid from a marine-derived Penicillium strain.
[So] Source:J Nat Prod;76(2):297-301, 2013 Feb 22.
[Is] ISSN:1520-6025
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A new chlorinated sesquiterpenoid analogue of fumagillin, ligerin (1), was isolated from a marine-derived strain of Penicillium, belonging to the subgenus Penicillium, along with the known compounds penicillic acid (2), orcinol, and orsellinic acid. Chemical structures were established by an interpretation of spectroscopic data including IR, UV, and HRESIMS, together with analyses of 1D and 2D NMR spectra and X-ray analysis for the determination of the absolute configuration. Ligerin (1) displayed strong inhibitory activity against an osteosarcoma cell line. This is the first report of the isolation of a fumagillin analogue from a marine-derived Penicillium strain.
[Mh] Termos MeSH primário: Antineoplásicos/isolamento & purificação
Antineoplásicos/farmacologia
Hidrocarbonetos Clorados/isolamento & purificação
Hidrocarbonetos Clorados/farmacologia
Penicillium/química
Sesquiterpenos/isolamento & purificação
Sesquiterpenos/farmacologia
[Mh] Termos MeSH secundário: Antineoplásicos/química
Cicloexanos/química
Cicloexanos/isolamento & purificação
Ensaios de Seleção de Medicamentos Antitumorais
Estuários
Ácidos Graxos Insaturados/química
Ácidos Graxos Insaturados/isolamento & purificação
França
Hidrocarbonetos Clorados/química
Biologia Marinha
Estrutura Molecular
Ressonância Magnética Nuclear Biomolecular
Osteossarcoma/tratamento farmacológico
Ácido Penicílico/isolamento & purificação
Sesquiterpenos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (Cyclohexanes); 0 (Fatty Acids, Unsaturated); 0 (Hydrocarbons, Chlorinated); 0 (Sesquiterpenes); 0 (ligerin); 7OW73204U1 (fumagillin); ONL14K3AFD (Penicillic Acid)
[Em] Mês de entrada:1304
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130131
[St] Status:MEDLINE
[do] DOI:10.1021/np3007364


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[PMID]:23243934
[Au] Autor:Goszcz H; Szczepanska L; Lech T; Groszek B
[Ad] Endereço:Oddzial Toksykologii i Chorób Wewnetrznych, Szpitala Specjalistycznego im. Ludwika Rydygiera w Krakowie.
[Ti] Título:[Long-term follow-up after intravenous injection of mercury--two cases report].
[Ti] Título:Wieloletnia obserwacja po dozylnym wstrzyknieciu rteci metalicznej--opis dwóch przypadków..
[So] Source:Przegl Lek;69(8):580-4, 2012.
[Is] ISSN:0033-2240
[Cp] País de publicação:Poland
[La] Idioma:pol
[Ab] Resumo:In this paper we present the long-term follow-up of two patients, after injection of metallic mercury. Case 1. In 1997, 29-years-old man injected himself to left elbow about 20 ml of metallic mercury by mistake (he was heroin abuser for short time). Mercury concentration in the blood was 400 microg/L. X-ray of the chest, abdomen and affected elbow area showed radiopaque foreign material (depots of mercury). Depots of mercury were also visible on the tricuspid valve in echocardiography. Mercury from the soft tissue left elbow pit was partially surgically removed. During 15 years follow-up two times chelating therapy was performed with d-penicyllamine and DMPS. In 2012, he was admitted to hospital next time. The blood and urine mercury concentration was still elevated (55.2 microg/L and 197 microg/L), mercury depots in the lung and abdomen were present. The signs and symptoms of CNS damage, like peripheral polyneuropathy and ataxia, were diagnosed. CT of brain did not revealed any changes, despite head trauma before 6 years. However neurological findings are typical for chronic mercury poisoning, it is not possible to determine whether these changes are directly related to mercury, because head trauma history, Case 2. In 2003, 16-years-old woman injected herself one month before, in suicidal attempts to both elbows several millilitres of metallic mercury. Mercury concentration in the blood was 56.2 microg/L, in urine 906 microg/L and in the hair 1.12 microg/g. Chest Xray showed depots of mercury in the lung. Mercury from the soft tissue was two times surgically removed. During 9 years two times chelating therapy was performed with d-penicyllamine and DMPS. After 9 years there is no symptoms of mercury poisoning. Mercury depots in the lung are still present. The blood and urine mercury concentration is low (13.7 microg/L and 2.53 microg/L). In mean time she gave birth two healthy children. Further patients evaluation is necessary.
[Mh] Termos MeSH primário: Quelantes/uso terapêutico
Intoxicação por Mercúrio/diagnóstico
Intoxicação por Mercúrio/tratamento farmacológico
[Mh] Termos MeSH secundário: Adolescente
Adulto
Feminino
Seguimentos
Seres Humanos
Injeções Intravenosas
Pulmão/química
Masculino
Mercúrio/administração & dosagem
Mercúrio/sangue
Mercúrio/urina
Intoxicação por Mercúrio/sangue
Intoxicação por Mercúrio/urina
Ácido Penicílico/análogos & derivados
Ácido Penicílico/uso terapêutico
Gravidez
Resultado da Gravidez
Tentativa de Suicídio
Resultado do Tratamento
Unitiol/uso terapêutico
Adulto Jovem
[Pt] Tipo de publicação:CASE REPORTS; ENGLISH ABSTRACT; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Chelating Agents); 34138-28-8 (penicillamide); 4076-02-2 (Unithiol); FXS1BY2PGL (Mercury); ONL14K3AFD (Penicillic Acid)
[Em] Mês de entrada:1301
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:121219
[St] Status:MEDLINE


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[PMID]:23081920
[Au] Autor:Montenegro TG; Rodrigues FA; Jimenez PC; Angelim AL; Melo VM; Rodrigues Filho E; de Oliveira Mda C; Costa-Lotufo LV
[Ad] Endereço:Departamento de Química Orgânica e Inorgânica, Universidade Federal do Ceará, Campus do Pici, P.O. Box 6044, 60.455-970, Fortaleza-CE, Brazil.
[Ti] Título:Cytotoxic activity of fungal strains isolated from the ascidian Eudistoma vannamei.
[So] Source:Chem Biodivers;9(10):2203-9, 2012 Oct.
[Is] ISSN:1612-1880
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:The cytotoxic activity at 50 µg/ml of extracts obtained from eleven fungal strains associated to Eudistoma vannamei, an endemic ascidian from Northeast Brazil, against two cell lines, i.e., the HCT-8 (colon cancer) and the MDA-MB-435 (melanoma) cell lines, was investigated. The most promising extract (EV10) was obtained from a fungus identified as Aspergillus sp. by molecular analysis and was selected for bioassay-guided isolation of its active principals. Large-scale fermentation of EV10 in potato-dextrose broth followed by chromatographic purification of the active extract from the liquid medium allowed the isolation of the isocoumarins mellein, cis-4-hydroxymellein, and trans-4-hydroxymellein, besides penicillic acid. All isolated compounds were tested for their cytotoxicity against the tumor cell lines MDA-MB-435 and HCT-8 and revealed penicillic acid as the only cytotoxic compound (cell growth inhibitions >95%).
[Mh] Termos MeSH primário: Fungos/química
Urocordados/microbiologia
[Mh] Termos MeSH secundário: Animais
Linhagem Celular Tumoral
Sobrevivência Celular/efeitos dos fármacos
Fungos/isolamento & purificação
Seres Humanos
Isocumarinas/química
Isocumarinas/isolamento & purificação
Isocumarinas/toxicidade
Ocratoxinas/química
Ocratoxinas/isolamento & purificação
Ocratoxinas/toxicidade
Ácido Penicílico/química
Ácido Penicílico/isolamento & purificação
Ácido Penicílico/toxicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Isocoumarins); 0 (Ochratoxins); 0 (ochracin); ONL14K3AFD (Penicillic Acid)
[Em] Mês de entrada:1303
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:121020
[St] Status:MEDLINE
[do] DOI:10.1002/cbdv.201100366


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[PMID]:21922431
[Au] Autor:Xu W; Xie P; Fan L; Cao C; Xi T; Zhou P
[Ad] Endereço:Laboratory of Analytical Biochemistry & Bio-separation, Key Laboratory of Microbiology of Ministry of Education of China, School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
[Ti] Título:Synthesis and characteristics of a novel artificial hapten using the copper mercaptide of penicillenic acid from penicillin G for immunoassay of heavy metal ions.
[So] Source:Sci China Life Sci;54(9):813-21, 2011 Sep.
[Is] ISSN:1869-1889
[Cp] País de publicação:China
[La] Idioma:eng
[Ab] Resumo:In this paper, we describe the synthesis of a novel copper ion hapten using the copper mercaptide of penicillenic acid (CMPA) derived from penicillin. Results from tests with immune rabbits indicate that: (i) A new antigen synthesized with CMPA has good stability and is safe for immunizing animals with no toxic phenomena being found in animal experiments; (ii) the immunogenic antigen (CMPA-BSA) can stimulate the immune system to produce specific antibodies with high titrations, up to 150000; and (iii) antibodies in antisera showed higher affinity to OVA-GSH-CuCl than OVA-GSH, which indicates that the antibodies have specific affinity towards copper ions. These results confirm that the novel hapten and relevant antigen for copper ion have been successfully synthesized, giving progress towards an immunoassay for copper ions in environmental and food samples.
[Mh] Termos MeSH primário: Acetilcisteína/química
Cobre/química
Haptenos/química
Imunoensaio/métodos
Metais Pesados/análise
Ácido Penicílico/química
Penicilina G/química
[Mh] Termos MeSH secundário: Animais
Masculino
Camundongos
Coelhos
Espectrofotometria Infravermelho
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Haptens); 0 (Metals, Heavy); 789U1901C5 (Copper); ONL14K3AFD (Penicillic Acid); Q42T66VG0C (Penicillin G); WYQ7N0BPYC (Acetylcysteine)
[Em] Mês de entrada:1201
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:110917
[St] Status:MEDLINE
[do] DOI:10.1007/s11427-011-4220-8


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[PMID]:20367389
[Au] Autor:Moslem M; Abd-Elsalam K; Yassin M; Bahkali A
[Ad] Endereço:Botany and Microbiology Department, College of Science, King Saud University, Riyadh, Saudi Arabia.
[Ti] Título:First morphomolecular identification of Penicillium griseofulvum and Penicillium aurantiogriseum toxicogenic isolates associated with blue mold on apple.
[So] Source:Foodborne Pathog Dis;7(7):857-61, 2010 Jul.
[Is] ISSN:1556-7125
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Postharvest blue mold decay caused by Penicillium spp. is the most important disease of fresh apple fruit in the world, which extend from the field to the store. Two new Penicillium spp. responsible for apple fruit decay were recovered. The morphological and molecular features of Penicillium griseofulvum and Penicillium aurantiogriseum isolated from apple fruits were characterized morphologically and molecularly. Pathogenicity test exhibited that both P. griseofulvum and P. aurantiogriseum were responsible for blue mold decay in storage apple fruits. Lesion diameter indicated that P. aurantiogriseum was more aggressive than P. griseofulvum. All tested isolates were able to synthesize citrinin in addition to patulin. Not all of the isolates belonging to the same species showed the same profile of secondary metabolites. Microsatellite-primed polymerase chain reaction was able to differentiate these isolates at the species level and divided the analyzed isolates into two genetically different groups. Little intraspecific variability was evident. Microsatellite-primed polymerase chain reaction analysis proved to be an objective, rapid, and reliable tool to identify Penicillium spp. involved in blue mold of apple. This is the first report of occurrence of P. griseofulvum and P. aurantiogriseum on imported apple fruits in Saudi Arabia.
[Mh] Termos MeSH primário: Frutas/microbiologia
Malus/microbiologia
Penicillium
Doenças das Plantas/microbiologia
[Mh] Termos MeSH secundário: Citrinina/biossíntese
Citrinina/química
Impressões Digitais de DNA
DNA Fúngico/química
DNA Fúngico/isolamento & purificação
Manipulação de Alimentos/métodos
Microbiologia de Alimentos
Repetições de Microssatélites
Micélio/ultraestrutura
Patulina/biossíntese
Patulina/química
Ácido Penicílico/química
Ácido Penicílico/metabolismo
Penicillium/isolamento & purificação
Penicillium/patogenicidade
Penicillium/fisiologia
Penicillium/ultraestrutura
Filogenia
Reação em Cadeia da Polimerase
Arábia Saudita
Especificidade da Espécie
Esporos Fúngicos/ultraestrutura
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Fungal); 3S697X6SNZ (Citrinin); 95X2BV4W8R (Patulin); ONL14K3AFD (Penicillic Acid)
[Em] Mês de entrada:1011
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:100407
[St] Status:MEDLINE
[do] DOI:10.1089/fpd.2009.0507


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[PMID]:20111864
[Au] Autor:Liaqat I; Bachmann RT; Sabri AN; Edyvean RG
[Ad] Endereço:Department of Microbiology and Molecular Genetics, University of the Punjab, Lahore, Pakistan. iramliaq@hotmail.com
[Ti] Título:Isolate-specific effects of patulin, penicillic Acid and EDTA on biofilm formation and growth of dental unit water line biofilm isolates.
[So] Source:Curr Microbiol;61(2):148-56, 2010 Aug.
[Is] ISSN:1432-0991
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Dental unit water line (DUWL) contamination by opportunistic pathogens has its significance in nosocomial infection of patients, health care workers, and life-threatening infections to immunocompromized persons. Recently, the quorum sensing (QS) system of DUWL isolates has been found to affect their biofilm-forming ability, making it an attractive target for antimicrobial therapy. In this study, the effect of two quorum-sensing inhibitory compounds (patulin; PAT, penicillic acid; PA) and EDTA on planktonic growth, AI-2 signalling and in vitro biofilm formation of Pseudomonas aeruginosa, Achromobacter xylosoxidans and Achromobacter sp. was monitored. Vibrio harveyi BB170 bioassay and crystal violet staining methods were used to detect the AI-2 monitoring and biofilm formation in DUWL isolates, respectively. The V. harveyi BB170 bioassay failed to induce bioluminescence in A. xylosoxidans and Achromobacter sp., while P. aeruginosa showed AI-2 like activity suggesting the need of some pretreatments prior to bioassay. All strains were found to form biofilms within 72 h of incubation. The QSIs/EDTA combination have isolate-specific effects on biofilm formation and in some cases it stimulated biofilm formation as often as it was inhibited. However, detailed information about the anti-biofilm effect of these compounds is still lacking.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Bactérias/efeitos dos fármacos
Biofilmes/efeitos dos fármacos
Ácido Edético/farmacologia
Patulina/farmacologia
Ácido Penicílico/farmacologia
Microbiologia da Água
[Mh] Termos MeSH secundário: Bactérias/classificação
Bactérias/crescimento & desenvolvimento
Bactérias/isolamento & purificação
Biofilmes/crescimento & desenvolvimento
DNA Bacteriano/química
DNA Bacteriano/genética
Consultórios Odontológicos
Homosserina/análogos & derivados
Homosserina/metabolismo
Seres Humanos
Lactonas/metabolismo
Dados de Sequência Molecular
Percepção de Quorum/efeitos dos fármacos
Análise de Sequência de DNA
Transdução de Sinais/efeitos dos fármacos
Abastecimento de Água
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (DNA, Bacterial); 0 (Lactones); 0 (N-octanoylhomoserine lactone); 6KA95X0IVO (Homoserine); 95X2BV4W8R (Patulin); 9G34HU7RV0 (Edetic Acid); ONL14K3AFD (Penicillic Acid)
[Em] Mês de entrada:1011
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:100130
[St] Status:MEDLINE
[do] DOI:10.1007/s00284-010-9591-8



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