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[PMID]:28379972
[Au] Autor:Burns AR; Bagg R; Yeo M; Luciani GM; Schertzberg M; Fraser AG; Roy PJ
[Ad] Endereço:The Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario, Canada.
[Ti] Título:The novel nematicide wact-86 interacts with aldicarb to kill nematodes.
[So] Source:PLoS Negl Trop Dis;11(4):e0005502, 2017 Apr.
[Is] ISSN:1935-2735
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Parasitic nematodes negatively impact human and animal health worldwide. The market withdrawal of nematicidal agents due to unfavourable toxicities has limited the available treatment options. In principle, co-administering nematicides at lower doses along with molecules that potentiate their activity could mitigate adverse toxicities without compromising efficacy. Here, we screened for new small molecules that interact with aldicarb, which is a highly effective treatment for plant-parasitic nematodes whose toxicity hampers its utility. From our collection of 638 worm-bioactive compounds, we identified 20 molecules that interact positively with aldicarb to either kill or arrest the growth of the model nematode Caenorhabditis elegans. We investigated the mechanism of interaction between aldicarb and one of these novel nematicides called wact-86. We found that the carboxylesterase enzyme GES-1 hydrolyzes wact-86, and that the interaction is manifested by aldicarb's inhibition of wact-86's metabolism by GES-1. This work demonstrates the utility of C. elegans as a platform to search for new molecules that can positively interact with industrial nematicides, and provides proof-of-concept for prospective discovery efforts.
[Mh] Termos MeSH primário: Aldicarb/farmacologia
Antinematódeos/farmacologia
Benzamidas/farmacologia
Benzofuranos/farmacologia
Proteínas de Caenorhabditis elegans/genética
Caenorhabditis elegans/efeitos dos fármacos
Hidrolases de Éster Carboxílico/genética
Nematoides/efeitos dos fármacos
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Antinematódeos/química
Proteínas de Caenorhabditis elegans/antagonistas & inibidores
Hidrolases de Éster Carboxílico/antagonistas & inibidores
Mutação
Alinhamento de Sequência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antinematodal Agents); 0 (Benzamides); 0 (Benzofurans); 0 (Caenorhabditis elegans Proteins); 0 (wact-86 compound); 8V071SH05P (Aldicarb); EC 3.1.1.- (Carboxylic Ester Hydrolases); EC 3.1.1.- (ges-1 protein, C elegans)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170516
[Lr] Data última revisão:
170516
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170406
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pntd.0005502


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[PMID]:27810542
[Au] Autor:Castro-Gutiérrez V; Masís-Mora M; Diez MC; Tortella GR; Rodríguez-Rodríguez CE
[Ad] Endereço:Centro de Investigación en Contaminación Ambiental (CICA), Universidad de Costa Rica, 2060 San José, Costa Rica; Centro de Investigación en Biología Celular y Molecular (CIBCM), Universidad de Costa Rica, 2060 San José, Costa Rica.
[Ti] Título:Aging of biomixtures: Effects on carbofuran removal and microbial community structure.
[So] Source:Chemosphere;168:418-425, 2017 Feb.
[Is] ISSN:1879-1298
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The aim of this work was to determine the efficiency of a straw/compost/soil biomixture for pesticide depuration during its aging and continuous use, for a period of over a year, based on its capacity to remove carbofuran (CFN), while simultaneously monitoring the variations in microbial community structure. Successive CFN spikings were applied in the biomixture at 6-week intervals, and the removal efficiency was determined 48 h post-application. Initially, only a discrete degradation performance was observed (9.9%), but one CFN application was sufficient to induce efficient elimination (>88.5%) of the pesticide at subsequent influxes for a period of over 6 months. A statistically significant reduction on CFN removal efficiency after this time was detected, reaching levels similar to the fresh-prepared biomixture (14.8%) at the end of the experiment. Simultaneous DGGE analyses showed only modest changes on microbial community patterns through time for both, bacteria and fungi. The clustering of genetic fingerprints in chronological groups corresponding to significantly different CFN degradation efficiencies indicates that biomixture aging changes not only the composition of microbial communities, but also their suitability to engage in pesticide degradation. Periodic substitution of straw/compost/soil biomixture in biopurification systems or regular provision of easily-degradable organic substrates should be considered to maintain an adequate depuration capacity on this system.
[Mh] Termos MeSH primário: Biodegradação Ambiental
Carbofurano/química
Microbiologia do Solo
Solo/química
[Mh] Termos MeSH secundário: Aldicarb/análise
Bactérias/metabolismo
Carbamatos/análise
DNA/análise
Eletroforese
Recuperação e Remediação Ambiental
Fungos/metabolismo
Metomil/análise
Resíduos de Praguicidas/análise
Praguicidas/análise
RNA Ribossômico 16S/análise
Poluentes do Solo/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Carbamates); 0 (Pesticide Residues); 0 (Pesticides); 0 (RNA, Ribosomal, 16S); 0 (Soil); 0 (Soil Pollutants); 1NQ08HN02S (Methomyl); 8V071SH05P (Aldicarb); 9007-49-2 (DNA); SKF77S6Y67 (Carbofuran); SWV4D62X9E (oxamyl)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161105
[St] Status:MEDLINE


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[PMID]:27750092
[Au] Autor:Rodríguez-Rodríguez CE; Madrigal-León K; Masís-Mora M; Pérez-Villanueva M; Chin-Pampillo JS
[Ad] Endereço:Centro de Investigación en Contaminación Ambiental (CICA), Universidad de Costa Rica, 2060 San José, Costa Rica. Electronic address: carlos.rodriguezrodriguez@ucr.ac.cr.
[Ti] Título:Removal of carbamates and detoxification potential in a biomixture: Fungal bioaugmentation versus traditional use.
[So] Source:Ecotoxicol Environ Saf;135:252-258, 2017 Jan.
[Is] ISSN:1090-2414
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The use of fungal bioaugmentation represents a promising way to improve the performance of biomixtures for the elimination of pesticides. The ligninolyitc fungus Trametes versicolor was employed for the removal of three carbamates (aldicarb, ALD; methomyl, MTM; and methiocarb, MTC) in defined liquid medium; in this matrix ALD and MTM showed similar half-lives (14d), nonetheless MTC exhibited a faster removal, with a half-life of 6.5d. Then the fungus was employed in the bioaugmentation of an optimized biomixture to remove the aforementioned carbamates plus carbofuran (CFN). Bioaugmented and non-bioaugmented systems removed over 99% ALD and MTM after 8d of treatment, nonetheless a slight initial delay in the removal was observed in the bioaugmented biomixtures (removal after 3d: ALD 87%/97%; MTM 86%/99%, in bioaugmented/non-bioaugmented systems). The elimination of the other carbamates was slower, but independent of the presence of the fungus: >98% for MTM after 35d and >99.5% for CFN after 22d. Though the bioaugmentation did not improve the removal capacity of the biomixture, it favored a lower production of transformation products at the first stages of the treatment, and in both cases, a marked decrease in the toxicity of the matrix was swiftly achieved along the process (from 435 to 448 TU to values <1TU in 16d).
[Mh] Termos MeSH primário: Carbamatos/metabolismo
Inseticidas/metabolismo
Trametes/metabolismo
[Mh] Termos MeSH secundário: Aldicarb/metabolismo
Biodegradação Ambiental
Carbofurano/análogos & derivados
Carbofurano/metabolismo
Meia-Vida
Inativação Metabólica
Lacase/análise
Metiocarb/metabolismo
Metomil/metabolismo
Solo/química
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Carbamates); 0 (Insecticides); 0 (Soil); 16709-30-1 (3-ketocarbofuran); 1NQ08HN02S (Methomyl); 7J7N7A61BJ (3-hydroxycarbofuran); 8V071SH05P (Aldicarb); EC 1.10.3.2 (Laccase); JI9431OS31 (Methiocarb); SKF77S6Y67 (Carbofuran)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161018
[St] Status:MEDLINE


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[PMID]:27839548
[Au] Autor:Kalinnikova TB; Kolsanova RR; Belova EB; Shagidullin RR; Gainutdinov MK
[Ad] Endereço:Research Institute for Problems of Ecology and Mineral Wealth Use of Tatarstan Academy of Sciences, Daurskaya str., 28, 420087 Kazan, Russia. Electronic address: tbkalinnikova@gmail.com.
[Ti] Título:Opposite effects of moderate heat stress and hyperthermia on cholinergic system of soil nematodes Caenorhabditis elegans and Caenorhabditis briggsae.
[So] Source:J Therm Biol;62(Pt A):37-49, 2016 Dec.
[Is] ISSN:0306-4565
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Cholinergic system plays important role in all functions of organisms of free-living soil nematodes C. elegans and C. briggsae. Using pharmacological analysis we showed the existence of two opposite responses of nematodes cholinergic system to moderate and extreme heat stress. Short-term (15min) noxious heat (31-32°C) caused activation of cholinergic synaptic transmission in C. elegans and C. briggsae organisms by sensitization of nicotinic ACh receptors. In contrast, hyperthermia blocked cholinergic synaptic transmission by inhibition of ACh secretion by neurons. The resistance of behavior to extreme high temperature (36-37°C) was significantly higher in C. briggsae than in C. elegans, and thermostability of cholinergic transmission correlated with resistance of behavior to hyperthermia. Activation of cholinergic transmission by moderate heat stress can be the reason of movement speed increase in such adaptive behavior as noxious heat escape. Inhibition of ACh release is one of reasons for behavior failure caused by extreme high temperature since partial inhibition of ACh-esterase by aldicarb protected C. elegans and C. briggsae behavior against hyperthermia. Antagonist of mAChRs atropine almost completely prevented the rise in behavior thermotolerance caused by aldicarb. Pilocarpine, agonist of mAChRs, protected nematodes behavior against hyperthermia similarly with aldicarb. Therefore it is evident that it is the deficiency of mAChRs activity that is the reason for nematodes' behavior failure by hyperthermia.
[Mh] Termos MeSH primário: Comportamento Animal/efeitos dos fármacos
Caenorhabditis/fisiologia
Febre/fisiopatologia
Resposta ao Choque Térmico
Receptores Nicotínicos/fisiologia
[Mh] Termos MeSH secundário: Acetilcolina/secreção
Aldicarb/administração & dosagem
Animais
Caenorhabditis elegans/fisiologia
Inibidores da Colinesterase/administração & dosagem
Levamisol/administração & dosagem
Nicotina/administração & dosagem
Agonistas Nicotínicos/administração & dosagem
Natação
Transmissão Sináptica/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cholinesterase Inhibitors); 0 (Nicotinic Agonists); 0 (Receptors, Nicotinic); 2880D3468G (Levamisole); 6M3C89ZY6R (Nicotine); 8V071SH05P (Aldicarb); N9YNS0M02X (Acetylcholine)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170331
[Lr] Data última revisão:
170331
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161115
[St] Status:MEDLINE


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[PMID]:27382721
[Au] Autor:Ding K; Xu W; Li K; Guo L; Sun J
[Ti] Título:[Dynamic behavior of aldicarb and its metabolites in cabbage by liquid chromatography-tandem mass spectrometry].
[So] Source:Se Pu;34(2):165-9, 2016 Feb.
[Is] ISSN:1000-8713
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:A liquid chromatography-tandem mass spectrometry ( LC-MS/MS ) method was developed for the study of dynamic behavior of aldicarb and its metabolite residues in cabbage. Aldicarb was applied onto cultivated cabbages. The pesticides concentrations were measured periodically (between application and harvest) , and modeled to illustrate the dynamic behavior. The results showed that the liner ranges of aldicarb and its metabolites were from 0. 005 to 0. 2 mg/L, and the recoveries ranged from 78. 9% to 108. 5% with the relative standard deviations of 2. 03%- 8. 91% (n = 8). The aldicarb in cabbage increased at first with the first-order kinetic equation model of c = 0. 020(0.136t) with the correlation coefficient (r2) of 0. 888, and then decreased with the equation of c = 0. 65e(-059t) with the r2 of 0. 979 and the half-life of 29. 1 d. The reducing processes of aldicarb-sulfone and aldicarb-sulfoxide both matched the first-order kinetic equations (c = 23. 4e(-0.044t) and c = 4. 54e(-0.027t) with r2 of 0. 916 and 0. 972 respectively. To meet the limitation requirement of 0. 01 mg/kg, 70. 7, 226. 6 and 176. 3 d were respectively necessary for aldicarb, aldicarb-sulfone and aldicarb-sulfoxide. Final residues of aldicarb-sulfone and aldicarb-sulfoxide were still more than the limitation requirements, indicating that aldicarb should not be used in vegetables of growth cycle shorter than 120 d. This study provided theoretical basis for dynamic behavior of aldicarb residue and its safe use in vegetables.
[Mh] Termos MeSH primário: Aldicarb/química
Brassica/metabolismo
Inseticidas/química
Resíduos de Praguicidas/análise
[Mh] Termos MeSH secundário: Cromatografia Líquida
Análise de Alimentos
Espectrometria de Massas em Tandem
[Pt] Tipo de publicação:ENGLISH ABSTRACT; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Insecticides); 0 (Pesticide Residues); 8V071SH05P (Aldicarb)
[Em] Mês de entrada:1607
[Cu] Atualização por classe:161018
[Lr] Data última revisão:
161018
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160708
[St] Status:MEDLINE


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Spinosa, Helenice de Souza
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[PMID]:27020045
[Au] Autor:de Siqueira A; Rodrigues KB; Gonçalves-Júnior V; Calefi AS; Fukushima AR; Cuevas SE; Spinosa Hde S; Maiorka PC
[Ad] Endereço:Department of Pathology, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil. Electronic address: siqueira.adriana.vp@gmail.com.
[Ti] Título:Exhumation of Wistar rats experimentally exposed to the carbamate pesticides aldicarb and carbofuran: A pathological and toxicological study.
[So] Source:Exp Toxicol Pathol;68(6):307-14, 2016 Jun.
[Is] ISSN:1618-1433
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Exhumation is required for the investigation of suspicions deaths when a body is buried and is usually performed under court order. Exhumation of animals is not a routine practice in forensic pathology. In this study, 30 male 70-day-old Wistar rats were experimentally exposed to the carbamate pesticides aldicarb and carbofuran. Toxicological, macroscopic and microscopic examinations were performed. Groups of 3 animals (2 exposed and 1 control) were evaluated at 24h, 3days, 5days, 7days and 10days post-mortem. In histopathological examination, the brain, liver, lungs and kidneys were assessed, and for toxicological analysis, the gastric contents, liver, vitreous humor, skeletal muscle and larvae (when available) were collected. The pesticides were detected by HPLC and quantified in the analyzed matrices, and a possible delay in tissue putrefaction due to the pesticides was observed. This study has revealed that it is possible to exhume animals for investigations of possible poisoning by carbamates and has demonstrated that the exhumation of an animal in a suspected case of poisoning should not be ruled out. The increasing demand for investigations of suspicious animal deaths, e.g., in cases of poisoning, will likely lead to an increase in the use of this type of procedure in veterinary pathology.
[Mh] Termos MeSH primário: Aldicarb/envenenamento
Carbofurano/envenenamento
Exumação/métodos
Inseticidas/envenenamento
Síndromes Neurotóxicas/veterinária
[Mh] Termos MeSH secundário: Aldicarb/análise
Animais
Carbofurano/análise
Cromatografia Líquida de Alta Pressão
Inseticidas/análise
Masculino
Ratos
Ratos Wistar
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Insecticides); 8V071SH05P (Aldicarb); SKF77S6Y67 (Carbofuran)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170314
[Lr] Data última revisão:
170314
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160330
[St] Status:MEDLINE


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[PMID]:26914800
[Au] Autor:Durão C; Machado MP
[Ad] Endereço:National Institute of Legal Medicine and Forensic Sciences, Lisbon, Portugal. drcarlosdurao@hotmail.com.
[Ti] Título:Death by chumbinho: aldicarb intoxication-regarding a corpse in decomposition.
[So] Source:Int J Legal Med;130(4):981-3, 2016 Jul.
[Is] ISSN:1437-1596
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Chumbinho is the popular name given to carbamate aldicarb (Temik), an insecticide commonly used in agriculture and highly toxic (LD50 = 0.9 mg/kg oral in rats) that has been sold clandestinely in several regions of Brazil. Chumbinho is sold illegally as raticide and is available in a formulation of small black granules which are easily mixed with food for criminal purposes, its use often being attributed to accidents and suicides, hence the importance of its inclusion in the toxicology studies of suspicious deaths. With the corpse putrefaction, many pathological and toxicological anatomical parameters are damaged or lost. This study emphasizes the importance of the gastric content observation, which in this case has, despite the advanced putrefaction, recognized the presence of chumbinho and guide its toxicological confirmation.
[Mh] Termos MeSH primário: Aldicarb/envenenamento
Inseticidas/envenenamento
Mudanças Depois da Morte
[Mh] Termos MeSH secundário: Aldicarb/análise
Aldicarb/química
Cromatografia Gasosa-Espectrometria de Massas
Conteúdo Gastrointestinal/química
Seres Humanos
Inseticidas/análise
Inseticidas/química
Masculino
Meia-Idade
Estrutura Molecular
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Insecticides); 8V071SH05P (Aldicarb)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170928
[Lr] Data última revisão:
170928
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160226
[St] Status:MEDLINE
[do] DOI:10.1007/s00414-016-1336-1


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[PMID]:26623950
[Au] Autor:De Paepe P; Colin P; Depuydt P; Decavele AS; De Smet J; Boussery K; Stove C; Benoit D; Verstraete A; Van Bocxlaer J; Buylaert W
[Ad] Endereço:a Department of Emergency Medicine , Ghent University Hospital , Ghent , Belgium.
[Ti] Título:Disulfiram inhibition of cyanide formation after acetonitrile poisoning.
[So] Source:Clin Toxicol (Phila);54(1):56-60, 2016.
[Is] ISSN:1556-9519
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:CONTEXT: Cyanide poisoning may be caused by acetonitrile, a common industrial organic solvent and laboratory agent. OBJECTIVE: To describe the potential use of disulfiram in treating acetonitrile poisoning in a human clinical case and to further study its effect in human liver microsomes in vitro. CASE DETAILS: A 30-year-old man initially presented with a cholinergic toxic syndrome following ingestion of aldicarb. Toxicological analysis revealed coingestion of ethanol. He subsequently developed severe metabolic acidosis caused by the cyanogenic compound acetonitrile which was erroneously interpreted as acetone in the chromatogram. After three treatments with hydroxocobalamin (5 g i.v.) and sodium thiosulfate (12.5 g i.v.) on days 2, 3, and 5, he had transient improvement but recurrent lactic acidosis. Treatment with disulfiram was associated on day 7 with resolution of metabolic acidosis and slowing of the decrease in acetonitrile concentration. He recovered from acetonitrile toxicity completely. The time course of acetonitrile, thiocyanate, and cyanide concentrations suggested that disulfiram inhibited cyanide formation. RESULTS: In vitro experiments with human liver microsomes showed the cyanide concentration was significantly lower after incubation with acetonitrile and disulfiram than acetonitrile alone (a mean 60% reduction in cyanide level). DISCUSSION: Although disulfiram was given late in the course of the poisoning it is possible that it contributed to the recovery.
[Mh] Termos MeSH primário: Acetonitrilos/envenenamento
Acidose Láctica/tratamento farmacológico
Cianetos/sangue
Dissulfiram/uso terapêutico
Envenenamento/tratamento farmacológico
[Mh] Termos MeSH secundário: Acetonitrilos/sangue
Acidose Láctica/sangue
Acidose Láctica/induzido quimicamente
Acidose Láctica/diagnóstico
Adulto
Consumo de Bebidas Alcoólicas/efeitos adversos
Consumo de Bebidas Alcoólicas/sangue
Aldicarb/sangue
Aldicarb/envenenamento
Biomarcadores/sangue
Concentração Alcoólica no Sangue
Inibidores da Colinesterase/sangue
Inibidores da Colinesterase/envenenamento
Etanol/efeitos adversos
Etanol/sangue
Seres Humanos
Masculino
Microssomos Hepáticos/efeitos dos fármacos
Microssomos Hepáticos/metabolismo
Envenenamento/sangue
Envenenamento/diagnóstico
Fatores de Tempo
Resultado do Tratamento
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acetonitriles); 0 (Biomarkers); 0 (Blood Alcohol Content); 0 (Cholinesterase Inhibitors); 0 (Cyanides); 3K9958V90M (Ethanol); 8V071SH05P (Aldicarb); TR3MLJ1UAI (Disulfiram); Z072SB282N (acetonitrile)
[Em] Mês de entrada:1604
[Cu] Atualização por classe:151215
[Lr] Data última revisão:
151215
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:151202
[St] Status:MEDLINE
[do] DOI:10.3109/15563650.2015.1101770


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[PMID]:26492468
[Au] Autor:Wang X; Hou T; Dong S; Liu X; Li F
[Ad] Endereço:College of Chemistry and Pharmaceutical Sciences, Qingdao Agricultural University, Qingdao 266109, PR China.
[Ti] Título:Fluorescence biosensing strategy based on mercury ion-mediated DNA conformational switch and nicking enzyme-assisted cycling amplification for highly sensitive detection of carbamate pesticide.
[So] Source:Biosens Bioelectron;77:644-9, 2016 Mar 15.
[Is] ISSN:1873-4235
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Pesticides are of great importance in agricultural and biological fields, but pesticide residues may harm the environment and human health. A highly sensitive fluorescent biosensor for the detection of carbamate pesticide has been developed based on acetylcholinesterase (AChE)-catalyzed hydrolysis product triggered Hg(2+) release coupled with subsequent nicking enzyme-induced cleavage of a duplex DNA for cycling amplification. In this protocol, two DNA probes, an unmodified single-stranded helper DNA probe 1 (HP1) and a quencher-fluorophore probe (QFP) are ingeniously designed. HP1 can be folded into hairpin configuration through T-Hg(2+)-T base pair formation. QFP, labeled with FAM and BHQ1 at its two terminals, contains the recognition sequence and the cleavage site of the nicking enzyme. In the presence of carbamate pesticide, the activity of AChE is inhibited, and the amount of the product containing the thiol group generated by the hydrolysis reaction of acetylthiocholine chloride (ACh) decreases, resulting in the release of a low concentration of Hg(2+). The number of HP1 that can be selectively unfolded would be reduced and the subsequent nicking enzyme-assisted cleavage processes would be affected, resulting in decreased fluorescence signals. The fluorescence intensity further decreases with the increase of the pesticide concentration. Therefore, the pesticide content can be easily obtained by monitoring the fluorescence signal change, which is inversely proportional to the logarithm of the pesticide concentration. The detection limit of aldicarb, the model analyte, is 3.3 µgL(-1), which is much lower than the Chinese National Standards or those previously reported. The as-proposed method has also been applied to detect carbamate pesticide residues in fresh ginger and artificial lake water samples with satisfactory results, which demonstrates that the method has great potential for practical application in biological or food safety field.
[Mh] Termos MeSH primário: Aldicarb/análise
DNA/química
DNA/ultraestrutura
Mercúrio/química
Praguicidas/análise
Espectrometria de Fluorescência/instrumentação
[Mh] Termos MeSH secundário: Carbamatos/análise
DNA/genética
Quebras de DNA de Cadeia Simples
Desenho de Equipamento
Análise de Falha de Equipamento
Técnicas de Amplificação de Ácido Nucleico/métodos
Conformação de Ácido Nucleico
Reprodutibilidade dos Testes
Sensibilidade e Especificidade
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Carbamates); 0 (Pesticides); 8V071SH05P (Aldicarb); 9007-49-2 (DNA); FXS1BY2PGL (Mercury)
[Em] Mês de entrada:1609
[Cu] Atualização por classe:151207
[Lr] Data última revisão:
151207
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151023
[St] Status:MEDLINE


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[PMID]:26824339
[Au] Autor:Botha CJ; Coetser H; Labuschagne L; Basson A
[Ad] Endereço:Department of Paraclinical Sciences, University of Pretoria, South Africa; Toxicology Laboratory, Agricultural Research Council-Onderstepoort Veterinary Institute, South Africa. christo.botha@up.ac.za.
[Ti] Título:Confirmed organophosphorus and carbamate pesticide poisonings in South African wildlife (2009-2014).
[So] Source:J S Afr Vet Assoc;86(1):1329, 2015 Dec 09.
[Is] ISSN:2224-9435
[Cp] País de publicação:South Africa
[La] Idioma:eng
[Ab] Resumo:During a six-year period (from January 2009 to December 2014), specimens collected from 344 cases of suspected organophosphorus and carbamate pesticide poisonings in wildlife, including birds, were submitted to the Toxicology Laboratory (ARC-OVI) for analysis. A positive diagnosis was made in 135 (39%) of these cases. The majority of cases were from birds, which included Cape vultures (Gyps coprotheres) and African white-backed vultures (Gyps africanus) and bateleur eagles (Terathopius ecaudatus). In one incident 49 vultures were killed when a farmer intentionally laced carcasses with carbofuran in an attempt to control jackal predation. There were 22 incidents of poisoning in helmeted guineafowl (Numida meleagris). On nine different occasions blue cranes (Anthropoides paradiseus) were poisoned, in one incident 14 birds were reported to have been killed. Over the period of investigation, there were 20 cases of poisoning involving mammalian species, the majority being vervet monkeys (Chlorocebus pygerythrus). The carbamate pesticides were responsible for 57 incidents of poisoning. Aldicarb, carbofuran and methomyl were detected in 26, 18 and 12 cases respectively. The majority of organophosphorus pesticide poisonings were caused by diazinon (n = 19), monocrotophos (n = 13) and methamidophos (n = 10).
[Mh] Termos MeSH primário: Animais Selvagens
Poluentes Ambientais/envenenamento
Praguicidas/envenenamento
[Mh] Termos MeSH secundário: Aldicarb/envenenamento
Animais
Carbofurano/envenenamento
Cromatografia Gasosa/veterinária
Espectrometria de Massas/veterinária
Metomil/envenenamento
Envenenamento/epidemiologia
Envenenamento/veterinária
África do Sul/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Environmental Pollutants); 0 (Pesticides); 1NQ08HN02S (Methomyl); 8V071SH05P (Aldicarb); SKF77S6Y67 (Carbofuran)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160130
[St] Status:MEDLINE
[do] DOI:10.4102/jsava.v86i1.1329



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