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[PMID]:27965115
[Au] Autor:Guerrero-Serrano G; Castanedo L; Cristóbal-Mondragón GR; Montalvo-Arredondo J; Riego-Ruíz L; DeLuna A; De Las Peñas A; Castaño I; Calera MR; Sánchez-Olea R
[Ad] Endereço:Instituto de Física, Universidad Autónoma de San Luis Potosí, Manuel Nava 6, Zona Univesitaria, C.P. 78290, San Luis Potosí, San Luis Potosí, Mexico.
[Ti] Título:Npa3/ScGpn1 carboxy-terminal tail is dispensable for cell viability and RNA polymerase II nuclear targeting but critical for microtubule stability and function.
[So] Source:Biochim Biophys Acta;1864(3):451-462, 2017 03.
[Is] ISSN:0006-3002
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Genetic deletion of the essential GTPase Gpn1 or replacement of the endogenous gene by partial loss of function mutants in yeast is associated with multiple cellular phenotypes, including in all cases a marked cytoplasmic retention of RNA polymerase II (RNAPII). Global inhibition of RNAPII-mediated transcription due to malfunction of Gpn1 precludes the identification and study of other cellular function(s) for this GTPase. In contrast to the single Gpn protein present in Archaea, eukaryotic Gpn1 possesses an extension of approximately 100 amino acids at the C-terminal end of the GTPase domain. To determine the importance of this C-terminal extension in Saccharomyces cerevisiae Gpn1, we generated yeast strains expressing either C-terminal truncated (gpn1ΔC) or full-length ScGpn1. We found that ScGpn1ΔC was retained in the cell nucleus, an event physiologically relevant as gpn1ΔC cells contained a higher nuclear fraction of the RNAPII CTD phosphatase Rtr1. gpn1ΔC cells displayed an increased size, a delay in mitosis exit, and an increased sensitivity to the microtubule polymerization inhibitor benomyl at the cell proliferation level and two cellular events that depend on microtubule function: RNAPII nuclear targeting and vacuole integrity. These phenotypes were not caused by inhibition of RNAPII, as in gpn1ΔC cells RNAPII nuclear targeting and transcriptional activity were unaffected. These data, combined with our description here of a genetic interaction between GPN1 and BIK1, a microtubule plus-end tracking protein with a mitotic function, strongly suggest that the ScGpn1 C-terminal tail plays a critical role in microtubule dynamics and mitotic progression in an RNAPII-independent manner.
[Mh] Termos MeSH primário: Núcleo Celular/metabolismo
Regulação Fúngica da Expressão Gênica
Microtúbulos/metabolismo
Proteínas Monoméricas de Ligação ao GTP/genética
RNA Polimerase II/genética
Proteínas de Saccharomyces cerevisiae/genética
[Mh] Termos MeSH secundário: Benomilo/farmacologia
Viabilidade Microbiana
Proteínas Associadas aos Microtúbulos/genética
Proteínas Associadas aos Microtúbulos/metabolismo
Microtúbulos/ultraestrutura
Proteínas Monoméricas de Ligação ao GTP/metabolismo
Domínios Proteicos
RNA Polimerase II/metabolismo
Saccharomyces cerevisiae/genética
Saccharomyces cerevisiae/metabolismo
Saccharomyces cerevisiae/ultraestrutura
Proteínas de Saccharomyces cerevisiae/metabolismo
Deleção de Sequência
Transdução de Sinais
Fatores de Transcrição/genética
Fatores de Transcrição/metabolismo
Transcrição Genética
Moduladores de Tubulina/farmacologia
Vacúolos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Bik1 protein, S cerevisiae); 0 (Microtubule-Associated Proteins); 0 (Rtr1 protein, S cerevisiae); 0 (Saccharomyces cerevisiae Proteins); 0 (Transcription Factors); 0 (Tubulin Modulators); EC 2.7.7.- (RNA Polymerase II); EC 3.6.1.- (Npa3 protein, S cerevisiae); EC 3.6.5.2 (Monomeric GTP-Binding Proteins); TLW21058F5 (Benomyl)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171006
[Lr] Data última revisão:
171006
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161215
[St] Status:MEDLINE


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[PMID]:27796927
[Au] Autor:Angel LP; Yusof MT; Ismail IS; Ping BT; Mohamed Azni IN; Kamarudin NH; Sundram S
[Ad] Endereço:Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia, 43400, Serdang, Selangor, Malaysia.
[Ti] Título:An in vitro study of the antifungal activity of Trichoderma virens 7b and a profile of its non-polar antifungal components released against Ganoderma boninense.
[So] Source:J Microbiol;54(11):732-744, 2016 Nov.
[Is] ISSN:1976-3794
[Cp] País de publicação:Korea (South)
[La] Idioma:eng
[Ab] Resumo:Ganoderma boninense is the causal agent of a devastating disease affecting oil palm in Southeast Asian countries. Basal stem rot (BSR) disease slowly rots the base of palms, which radically reduces productive lifespan of this lucrative crop. Previous reports have indicated the successful use of Trichoderma as biological control agent (BCA) against G. boninense and isolate T. virens 7b was selected based on its initial screening. This study attempts to decipher the mechanisms responsible for the inhibition of G. boninense by identifying and characterizing the chemical compounds as well as the physical mechanisms by T. virens 7b. Hexane extract of the isolate gave 62.60% ± 6.41 inhibition against G. boninense and observation under scanning electron microscope (SEM) detected severe mycelial deformation of the pathogen at the region of inhibition. Similar mycelia deformation of G. boninense was observed with a fungicide treatment, Benlate indicating comparable fungicidal effect by T. virens 7b. Fraction 4 and 5 of hexane active fractions through preparative thin layer chromatography (P-TLC) was identified giving the best inhibition of the pathogen. These fractions comprised of ketones, alcohols, aldehydes, lactones, sesquiterpenes, monoterpenes, sulphides, and free fatty acids profiled through gas chromatography mass spectrometry detector (GC/MSD). A novel antifungal compound discovery of phenylethyl alcohol (PEA) by T. virens 7b is reported through this study. T. virens 7b also proved to be an active siderophore producer through chrome azurol S (CAS) agar assay. The study demonstrated the possible mechanisms involved and responsible in the successful inhibition of G. boninense.
[Mh] Termos MeSH primário: Antifúngicos/farmacologia
Agentes de Controle Biológico/química
Ganoderma/efeitos dos fármacos
Trichoderma/química
[Mh] Termos MeSH secundário: Antifúngicos/química
Antifúngicos/isolamento & purificação
Antifúngicos/metabolismo
Benomilo/farmacologia
Agentes de Controle Biológico/isolamento & purificação
Agentes de Controle Biológico/farmacologia
Microscopia Eletrônica de Varredura
Micélio/efeitos dos fármacos
Micélio/ultraestrutura
Álcool Feniletílico/química
Álcool Feniletílico/isolamento & purificação
Álcool Feniletílico/farmacologia
Doenças das Plantas/microbiologia
Sideróforos/biossíntese
Trichoderma/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antifungal Agents); 0 (Biological Control Agents); 0 (Siderophores); ML9LGA7468 (Phenylethyl Alcohol); TLW21058F5 (Benomyl)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:171109
[Lr] Data última revisão:
171109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161101
[St] Status:MEDLINE


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[PMID]:27784618
[Au] Autor:Jang Y; Lee AY; Kim JE; Jeong SH; Kim JS; Cho MH
[Ad] Endereço:College of Veterinary Medicine, Seoul National University, Seoul 08826, Republic of Korea.
[Ti] Título:Benomyl-induced effects of ORMDL3 overexpression via oxidative stress in human bronchial epithelial cells.
[So] Source:Food Chem Toxicol;98(Pt B):100-106, 2016 Dec.
[Is] ISSN:1873-6351
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The respiratory system is a major site of exposure route during pesticide use. Although pesticide exposure is associated with chronic respiratory diseases including asthma, the underlying pathophysiological mechanism remains to be elucidated. In this study, we investigated the in vitro effects of benomyl-induced ORMDL3 overexpression on the toxicological mechanism using the human bronchial epithelial cell line 16HBE14o-. Benomyl increased reactive oxygen species and Ca levels, and asthma-related ADAM33 and ORMDL3 expression in 16HBE14o- cells. Considering the change in Ca level and protein expression, we focused on ORMDL3 to elucidate the mechanism of benomyl-induced asthma. Antioxidant treatment showed that benomyl-induced ORMDL3 and endoplasmic reticulum stress could be triggered by oxidative stress. Furthermore, ORMDL3 knockdown alleviated the effects of benomyl on intracellular Ca , and the expression of metalloproteinases, and proinflammatory cytokines involved in the pathogenesis of asthma. In conclusion, our results suggest that benomyl-induced ORMDL3 overexpression via oxidative stress might be a mechanism involved in asthma. Moreover, antioxidants and alleviating mechanisms that reduce ORMDL3 levels could serve as promising therapeutic targets for pesticide-induced asthma.
[Mh] Termos MeSH primário: Proteínas ADAM/metabolismo
Benomilo/farmacologia
Brônquios/metabolismo
Células Epiteliais/metabolismo
Regulação da Expressão Gênica/efeitos dos fármacos
Proteínas de Membrana/metabolismo
Estresse Oxidativo/efeitos dos fármacos
[Mh] Termos MeSH secundário: Proteínas ADAM/genética
Antioxidantes/farmacologia
Western Blotting
Brônquios/citologia
Brônquios/efeitos dos fármacos
Sobrevivência Celular/efeitos dos fármacos
Células Cultivadas
Citocinas/genética
Citocinas/metabolismo
Células Epiteliais/citologia
Células Epiteliais/efeitos dos fármacos
Seres Humanos
Proteínas de Membrana/genética
RNA Mensageiro/genética
Espécies Reativas de Oxigênio/metabolismo
Reação em Cadeia da Polimerase em Tempo Real
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Moduladores de Tubulina/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Cytokines); 0 (Membrane Proteins); 0 (ORMDL3 protein, human); 0 (RNA, Messenger); 0 (Reactive Oxygen Species); 0 (Tubulin Modulators); EC 3.4.24.- (ADAM Proteins); EC 3.4.24.- (ADAM33 protein, human); TLW21058F5 (Benomyl)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170918
[Lr] Data última revisão:
170918
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161105
[St] Status:MEDLINE


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[PMID]:27198228
[Au] Autor:Schibler A; Koutelou E; Tomida J; Wilson-Pham M; Wang L; Lu Y; Cabrera AP; Chosed RJ; Li W; Li B; Shi X; Wood RD; Dent SY
[Ad] Endereço:Program in Genes and Development, The University of Texas M.D. Anderson Cancer Center, Houston, Texas 77030, USA; The Graduate School of Biomedical Sciences (GSBS) at Houston, Houston, Texas 77030, USA; Center for Cancer Epigenetics, The University of Texas M.D. Anderson Cancer Center, Houston, Texa
[Ti] Título:Histone H3K4 methylation regulates deactivation of the spindle assembly checkpoint through direct binding of Mad2.
[So] Source:Genes Dev;30(10):1187-97, 2016 05 15.
[Is] ISSN:1549-5477
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Histone H3 methylation on Lys4 (H3K4me) is associated with active gene transcription in all eukaryotes. In Saccharomyces cerevisiae, Set1 is the sole lysine methyltransferase required for mono-, di-, and trimethylation of this site. Although H3K4me3 is linked to gene expression, whether H3K4 methylation regulates other cellular processes, such as mitosis, is less clear. Here we show that both Set1 and H3K4 mutants display a benomyl resistance phenotype that requires components of the spindle assembly checkpoint (SAC), including Bub3 and Mad2. These proteins inhibit Cdc20, an activator of the anaphase-promoting complex/cyclosome (APC/C). Mutations in Cdc20 that block Mad2 interactions suppress the benomyl resistance of both set1 and H3K4 mutant cells. Furthermore, the HORMA domain in Mad2 directly binds H3, identifying a new histone H3 "reader" motif. Mad2 undergoes a conformational change important for execution of the SAC. We found that the closed (active) conformation of both yeast and human Mad2 is capable of binding methylated H3K4, but, in contrast, the open (inactive) Mad2 conformation limits interaction with methylated H3. Collectively, our data indicate that interactions between Mad2 and H3K4 regulate resolution of the SAC by limiting closed Mad2 availability for Cdc20 inhibition.
[Mh] Termos MeSH primário: Histonas/metabolismo
Pontos de Checagem da Fase M do Ciclo Celular/genética
Proteínas Mad2/metabolismo
[Mh] Termos MeSH secundário: Benomilo/farmacologia
Proteínas Cdc20/genética
Proteínas Cdc20/metabolismo
Resistência a Medicamentos/genética
Histona-Lisina N-Metiltransferase/genética
Histona-Lisina N-Metiltransferase/metabolismo
Histonas/genética
Seres Humanos
Pontos de Checagem da Fase M do Ciclo Celular/efeitos dos fármacos
Metilação
Mutação
Ligação Proteica/genética
Conformação Proteica
Proteínas Recombinantes/genética
Proteínas Recombinantes/metabolismo
Saccharomyces cerevisiae/citologia
Saccharomyces cerevisiae/efeitos dos fármacos
Saccharomyces cerevisiae/genética
Saccharomyces cerevisiae/metabolismo
Proteínas de Saccharomyces cerevisiae/genética
Proteínas de Saccharomyces cerevisiae/metabolismo
Fuso Acromático/genética
Fuso Acromático/patologia
Ativação Transcricional/efeitos dos fármacos
Ativação Transcricional/fisiologia
Moduladores de Tubulina/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Cdc20 Proteins); 0 (Histones); 0 (Mad2 Proteins); 0 (Recombinant Proteins); 0 (Saccharomyces cerevisiae Proteins); 0 (Tubulin Modulators); EC 2.1.1.43 (Histone-Lysine N-Methyltransferase); EC 2.1.1.43 (SET1 protein, S cerevisiae); TLW21058F5 (Benomyl)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170930
[Lr] Data última revisão:
170930
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160521
[St] Status:MEDLINE
[do] DOI:10.1101/gad.278887.116


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[PMID]:26976730
[Au] Autor:Zhou Y; Xu J; Zhu Y; Duan Y; Zhou M
[Ad] Endereço:All authors: College of Plant Protection, Nanjing Agricultural University, Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects, Ministry of Agriculture, Nanjing, China; and second author: College of Forestry, Henan University of Science and Technology, Tianjing Rd No 70, 47
[Ti] Título:Mechanism of Action of the Benzimidazole Fungicide on Fusarium graminearum: Interfering with Polymerization of Monomeric Tubulin But Not Polymerized Microtubule.
[So] Source:Phytopathology;106(8):807-13, 2016 Aug.
[Is] ISSN:0031-949X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Tubulins are the proposed target of clinically relevant anticancer drugs, anthelmintic, and fungicide. ß2-tubulin of the plant pathogen Fusarium graminearum was considered as the target of benzimidazole compounds by homology modeling in our previous work. In this study, α1-, α2-, and ß2-tubulin of F. graminearum were produced in Escherichia coli. Three benzimidazole compounds (carbendazim, benomyl, and thiabendazole) interacted with the recombinant ß2-tubulin and reduced the maximum fluorescence intensity of 2 µM ß2-tubulin 47, 50, and 25%, respectively, at saturation of compound-tubulin complexes. Furthermore, carbendazim significantly inhibited the polymerization of α1-/ß2-tubulins and α2-/ß2-tubulins 90.9 ± 0.4 and 93.5 ± 0.05%, respectively, in vitro. A similar result appeared with benomyl on the polymerization of α1-/ß2-tubulins and α2-/ß2-tubulins at 89.9 ± 0.1% and 92.6 ± 1.2% inhibition ratios, respectively. In addition, thiabendazole inhibited 81.6 ± 1% polymerization of α1-/ß2-tubulins, whereas it had less effect on α2-/ß2-tubulin polymerization, with 20.1 ± 1.9% inhibition ratio. However, the three compounds cannot destabilize the polymerized microtubule. To illuminate the issue, mapping the carbendazim binding sites and ß/α subunit interface on ß/α-tubulin complexes by homology modeling showed that the two domains were closed to each other. Understanding the nature of the interaction between benzimidazole compounds and F. graminearum tubulin is fundamental for the development of tubulin-specific anti-F. graminearum compounds.
[Mh] Termos MeSH primário: Benzimidazóis/farmacologia
Fungicidas Industriais/farmacologia
Fusarium/efeitos dos fármacos
Microtúbulos/fisiologia
Tubulina (Proteína)/fisiologia
[Mh] Termos MeSH secundário: Benomilo/farmacologia
Carbamatos/farmacologia
Proteínas Fúngicas/metabolismo
Regulação Fúngica da Expressão Gênica/efeitos dos fármacos
Modelos Moleculares
Polimerização/efeitos dos fármacos
Ligação Proteica
Conformação Proteica
Redobramento de Proteína
Proteínas Recombinantes
Tiabendazol/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Benzimidazoles); 0 (Carbamates); 0 (Fungal Proteins); 0 (Fungicides, Industrial); 0 (Recombinant Proteins); 0 (Tubulin); H75J14AA89 (carbendazim); N1Q45E87DT (Thiabendazole); TLW21058F5 (Benomyl)
[Em] Mês de entrada:1609
[Cu] Atualização por classe:160722
[Lr] Data última revisão:
160722
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160316
[St] Status:MEDLINE
[do] DOI:10.1094/PHYTO-08-15-0186-R


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[PMID]:26975770
[Au] Autor:Arunachalam Palaniyandi S; Yang SH; Suh JW
[Ad] Endereço:Graduate School of Interdisciplinary Program of Biomodulation, Myongji University, Yongin 17058, Republic of Korea.
[Ti] Título:Foliar Application of Extract from an Azalomycin-Producing Streptomyces malaysiensis Strain MJM1968 Suppresses Yam Anthracnose Caused by Colletotrichum gloeosporioides.
[So] Source:J Microbiol Biotechnol;26(6):1103-8, 2016 Jun 28.
[Is] ISSN:1738-8872
[Cp] País de publicação:Korea (South)
[La] Idioma:eng
[Ab] Resumo:Yam anthracnose caused by Colletotrichum gloeosporioides (C.g) is the most devastating disease of yam (Dioscorea sp.). In the present study, we evaluated the culture filtrate extract (CFE) of azalomycin-producing Streptomyces malaysiensis strain MJM1968 for the control of yam anthracnose. MJM1968 showed strong antagonistic activity against C.g in vitro. Furthermore, the MJM1968 CFE was tested for inhibition of spore germination in C.g, where it completely inhibited spore germination at a concentration of 50 µg/ml. To assess the in planta efficacy of the CFE and spores of MJM1968 against C.g, a detached leaf bioassay was conducted, which showed both the treatments suppressed anthracnose development on detached yam leaves. Furthermore, a greenhouse study was conducted to evaluate the CFE from MJM1968 as a fungicide for the control of yam anthracnose. The CFE non-treated plants showed a disease severity of >92% after 90 days of artificial inoculation with C.g, whereas the disease severity of CFE-treated and benomyl-treated yam plants was reduced to 26% and 15%, respectively, after 90 days. Analysis of the yam tubers from the CFE-treated and non-treated groups showed that tubers from the CFE-treated plants were larger than that of non-treated plants, which produced abnormal smaller tubers typical of anthracnose. This study demonstrated the utility of the CFE from S. malaysiensis strain MJM1968 as a biofungicide for the control of yam anthracnose.
[Mh] Termos MeSH primário: Antibiose
Colletotrichum/fisiologia
Dioscorea/microbiologia
Fungicidas Industriais/farmacologia
Macrolídeos/metabolismo
Doenças das Plantas/prevenção & controle
Streptomyces/fisiologia
[Mh] Termos MeSH secundário: Benomilo/farmacologia
Meios de Cultura/química
Doenças das Plantas/microbiologia
Folhas de Planta/efeitos dos fármacos
Folhas de Planta/microbiologia
Tubérculos/crescimento & desenvolvimento
Tubérculos/microbiologia
Análise de Sequência de DNA
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Culture Media); 0 (Fungicides, Industrial); 0 (Macrolides); 1CAF8865TM (elaiophylin); TLW21058F5 (Benomyl)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170316
[Lr] Data última revisão:
170316
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160316
[St] Status:MEDLINE
[do] DOI:10.4014/jmb.1601.01018


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[PMID]:26523765
[Au] Autor:Shimizu Y; Nagai M; Yeasmin AM; Koike N; Talukdar MW; Ushimaru T
[Ad] Endereço:a Graduate School of Science and Technology , Shizuoka University , Shizuoka , Japan.
[Ti] Título:Elucidation of novel budding yeast separase mutants.
[So] Source:Biosci Biotechnol Biochem;80(3):473-8, 2016.
[Is] ISSN:1347-6947
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The mitotic separase cleaves Scc1 in cohesin to allow sister chromatids to separate from each other upon anaphase onset. Separase is also required for DNA damage repair. Here, we isolated and characterized 10 temperature-sensitive (ts) mutants of separase ESP1 in the budding yeast Saccharomyces cerevisiae. All mutants were defective in sister chromatid separation at the restricted temperature. Some esp1-ts mutants were hypersensitive to the microtubule poison benomyl and/or the DNA-damaging agent bleomycin. Overexpression of securin alleviated the growth defect in some esp1-ts mutants, whereas it rather exacerbated it in others. The Drosophila Pumilio homolog MPT5 was isolated as a high-dosage suppressor of esp1-ts cells. We discuss various features of separase based on these findings.
[Mh] Termos MeSH primário: Mutação
Saccharomyces cerevisiae/enzimologia
Separase/genética
[Mh] Termos MeSH secundário: Benomilo/farmacologia
Bleomicina/farmacologia
Proteínas de Fluorescência Verde/genética
Pressão Osmótica
Saccharomyces cerevisiae/efeitos dos fármacos
Separase/metabolismo
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
11056-06-7 (Bleomycin); 147336-22-9 (Green Fluorescent Proteins); EC 3.4.22.49 (Separase); TLW21058F5 (Benomyl)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151103
[St] Status:MEDLINE
[do] DOI:10.1080/09168451.2015.1101337


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[PMID]:26349399
[Au] Autor:Kini S; Bahadur D; Panda D
[Ti] Título:Mechanism of Anti-Cancer Activity of Benomyl Loaded Nanoparticles in Multidrug Resistant Cancer Cells.
[So] Source:J Biomed Nanotechnol;11(5):877-89, 2015 May.
[Is] ISSN:1550-7033
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Polymeric chitosan-poly(D,L-lactide-co-glycolide) nanoparticles loaded with benomyl as anticancer drug formulation against multidrug-resistant EMT6/AR1 cells were synthesized by amine-carboxylate reaction. Using transmission electron microscopy, the average size of chitosan-poly(D,L-lactide-co-glycolide) nanoparticles and benomyl-encapsulated polymeric chitosan-poly(D,L-lactide-co-glycolide) nanoparticles was estimated to be 155 ± 20 nm and 160 ± 25 nm, respectively. Fourier transform infrared spectroscopy revealed that poly(D,L-lactide-co-glycolide) and chitosan are linked by covalent bonds. Zeta potentials of benomyl-encapsulated polymeric chitosan-poly(D,L-lactide-co-glycolide) nanoparticles at pH 4, 7.2, and 10 were 30 ± 1.8, 19 ± 0.65, and -22 ± 0.15 mV, respectively, indicating the formation of stable, hydrophilic nanoparticles. The release of benomyl from benomyl-encapsulated polymeric chitosan-poly(D,L-lactide-co-glycolide) nanoparticles followed pH-dependent kinetics. The uptake of fluorescein isothiocyanate-labeled chitosan-poly(D,L-lactide-co-glycolide) nanoparticles was concentration-dependent in both MCF-7 and multidrug-resistant EMT6/AR1 cells. EMT6/AR1 cells showed 10-fold higher resistance to benomyl compared to MCF-7 cells; in contrast, benomyl-encapsulated polymeric chitosan-poly(D,L-lactide-co-glycolide) nanoparticles effectively inhibited proliferation of MCF-7 and EMT6/AR1 cells with a half-maximal inhibitory concentration of 4 ± 0.5 and 9 ± 0.5 pM, respectively. In the presence of a P-glycoprotein inhibitor, the activity of benomyl was increased, suggesting that benomyl is a substrate for P-glycoprotein. Further, benomyl-encapsulated polymeric chitosan-poly(D,L-lactide-co-glycolide) nanoparticles depoly-merized microtubules both in interphase and mitosis. It blocked cell cycle progression at G2/M and induced apoptosis in EMT6/AR1 cells, suggesting that benomyl-encapsulated polymeric chitosan-poly(D,L-lactide-co-glycolide) nanoparticles have chemotherapeutic activity against multidrug-resistant cancer cells.
[Mh] Termos MeSH primário: Antineoplásicos/administração & dosagem
Benomilo/administração & dosagem
Resistência a Medicamentos Antineoplásicos
Nanopartículas/uso terapêutico
Neoplasias/tratamento farmacológico
[Mh] Termos MeSH secundário: Animais
Antineoplásicos/farmacocinética
Apoptose/efeitos dos fármacos
Benomilo/farmacocinética
Ciclo Celular/efeitos dos fármacos
Sobrevivência Celular/efeitos dos fármacos
Portadores de Fármacos
Resistência a Medicamentos Antineoplásicos/efeitos dos fármacos
Seres Humanos
Células MCF-7
Camundongos
Nanopartículas/química
Neoplasias/metabolismo
Células Tumorais Cultivadas
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (Drug Carriers); TLW21058F5 (Benomyl)
[Em] Mês de entrada:1510
[Cu] Atualização por classe:150909
[Lr] Data última revisão:
150909
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150910
[St] Status:MEDLINE


  9 / 481 MEDLINE  
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[PMID]:26149821
[Au] Autor:Pech P; Heneberg P
[Ad] Endereço:University of Hradec Králové, Faculty of Science, Hradec Králové, Czech Republic.
[Ti] Título:Benomyl treatment decreases fecundity of ant queens.
[So] Source:J Invertebr Pathol;130:61-3, 2015 Sep.
[Is] ISSN:1096-0805
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Methyl benzimidazole carbamate fungicides, including benomyl, are widely used in agriculture, and to eliminate entomopathogenic infections. We treated queens of Myrmica rubra (Hymenoptera:Formicidae) infected or not by Rickia wasmannii (Laboulbeniales:Laboulbeniaceae) with benomyl, 1mg/ml p.o. for six weeks. Benomyl did not treat the infection, and the treatment alone caused strong decrease in the fecundity of control healthy queens from 18.0±8.4 to 3.7±5.2eggs per healthy queen. This is the first evidence on severe adverse effects of methyl benzimidazole carbamate fungicide on the fecundity of insects, which might be responsible for altered species composition of ant assemblages in the cultural landscape.
[Mh] Termos MeSH primário: Formigas/efeitos dos fármacos
Formigas/parasitologia
Benomilo/toxicidade
Fungicidas Industriais/toxicidade
[Mh] Termos MeSH secundário: Animais
Feminino
Fertilidade/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Fungicides, Industrial); TLW21058F5 (Benomyl)
[Em] Mês de entrada:1611
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150708
[St] Status:MEDLINE


  10 / 481 MEDLINE  
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[PMID]:25947038
[Au] Autor:Wang Q; Yang J; Dong Y; Zhang L
[Ad] Endereço:College of Chemistry, Liaoning University, 66 Chongshan Middle Road, Shenyang, Liaoning 110036, People's Republic of China.
[Ti] Título:One-Step Fabrication of a Multifunctional Magnetic Nickel Ferrite/Multi-walled Carbon Nanotubes Nanohybrid-Modified Electrode for the Determination of Benomyl in Food.
[So] Source:J Agric Food Chem;63(19):4746-53, 2015 May 20.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Benomyl, as one kind of agricultural pesticide, has adverse impact on human health and the environment. It is urgent to develop effective and rapid methods for quantitative determination of benomyl. A simple and sensitive electroanalytical method for determination of benomyl using a magnetic nickel ferrite (NiFe2O4)/multi-walled carbon nanotubes (MWCNTs) nanohybrid-modified glassy carbon electrode (GCE) was presented. The electrocatalytic properties and electroanalysis of benomyl on the modified electrode were investigated by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). In the phosphate-buffered saline (PBS) of pH 6.0, this constructed biosensor exhibited two linear relationships with the benomyl concentration range from 1.00 × 10(-7) to 5.00 × 10(-7) mol/L and from 5.00 × 10(-7) to 1.00 × 10(-5) mol/L, respectively. The detection limit was 2.51 × 10(-8) mol/L (S/N = 3). Moreover, the proposed method was successfully applied to determine benomyl in real samples with satisfactory results. The NiFe2O4/MWCNTs/GCE showed good reproducibility and stability, excellent catalytic activity, and anti-interference.
[Mh] Termos MeSH primário: Agaricales/química
Benomilo/análise
Técnicas Biossensoriais/métodos
Contaminação de Alimentos/análise
Malus/química
Praguicidas/análise
Spinacia oleracea/química
[Mh] Termos MeSH secundário: Técnicas Biossensoriais/instrumentação
Eletrodos
Compostos Férricos/química
Fenômenos Magnéticos
Nanotubos de Carbono/química
Níquel/química
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Ferric Compounds); 0 (Nanotubes, Carbon); 0 (Pesticides); 0 (nickel ferrite); 7OV03QG267 (Nickel); TLW21058F5 (Benomyl)
[Em] Mês de entrada:1510
[Cu] Atualização por classe:150520
[Lr] Data última revisão:
150520
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150508
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.5b00973



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