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[PMID]:28456303
[Au] Autor:Han Y; Song S; Wu H; Zhang J; Ma E
[Ad] Endereço:Institute of Applied Biology, Shanxi University, 92 Wucheng Road, Taiyuan, Shanxi 030006, China; School of Life Science, Shanxi University, 92 Wucheng Road, Taiyuan, Shanxi 030006, China; Shanxi Key Laboratory of Integrated Pest Management in Agriculture, 92 Wucheng Road, Taiyuan, Shanxi 030006, Chi
[Ti] Título:Antioxidant enzymes and their role in phoxim and carbaryl stress in Caenorhabditis elegans.
[So] Source:Pestic Biochem Physiol;138:43-50, 2017 May.
[Is] ISSN:1095-9939
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Pesticide exposure can induce oxidative stress and cause changes to antioxidant enzymes in living organisms. In the present study, the effects of phoxim (an organophosphorus insecticide) and carbaryl (a carbamate insecticide) on antioxidant enzyme activity and gene expression were investigated in the model organism Caenorhabditis elegans. The results show that phoxim exposure can induce superoxide dismutase (SOD) and catalase (CAT) activities and decrease glutathione peroxidase (GPx) activity at lower concentrations. The expression levels of sod-3, sod-5, ctl-1, gpx-6, and gpx-8 were up-regulated after treatment with phoxim. The mRNA expression levels of sod-5, ctl-1 and gpx-6 were increased approximately 70-, 170- and 130-fold, respectively, in the 0.25mM treatment group compared to the control group. Carbaryl exposure decreased SOD activity and induced CAT and GPx activities. The addition of carbaryl up-regulated the expression of sod-5, ctl-1, ctl-3 and gpx-8. Specifically, ctl-1 expression increased approximately 10-fold, and gpx-8 expression increased <30-fold in the 0.5mM treatment group relative to the control group. The transcript level of sod-5 increased >20-fold, and ctl-3 increased approximately 10-fold in the 1mM treatment group. The functions of the antioxidant enzymes during oxidative stress caused by the two insecticides were investigated using deletion mutants. The LC values phoxim for the of sod-3 (tm760), sod-5 (tm1146), ctl-1 (ok1242), ctl-3 (ok2042) and gpx-8 (tm2108) mutant strains were lower than those observed for the N2 strain. The LC values of carbaryl for the ctl-1 (ok1242), ctl-3 (ok2042) and gpx-6 (tm2535) deletion mutant strains decreased in comparison to the N2 strain. The results suggest that these two insecticides caused oxidative stress and changed altered the antioxidant enzyme activities and their gene expressions in C. elegans. The sod-3, sod-5, ctl-1, ctl-3, gpx-6, and gpx-8 encoding enzymes may play roles in defending cells from oxidative stress caused by these two insecticides.
[Mh] Termos MeSH primário: Antioxidantes/metabolismo
Caenorhabditis elegans/efeitos dos fármacos
Carbaril/toxicidade
Inseticidas/toxicidade
Compostos Organotiofosforados/toxicidade
Estresse Fisiológico/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Caenorhabditis elegans/enzimologia
Regulação Enzimológica da Expressão Gênica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Insecticides); 0 (Organothiophosphorus Compounds); 6F5V775VPO (phoxim); R890C8J3N1 (Carbaryl)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170501
[St] Status:MEDLINE


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[PMID]:28289805
[Au] Autor:Emanuela F; Giuseppe S; Stefano G; Mattia B; Mauro M
[Ad] Endereço:National Research Council (CNR), Institute of Marine Science (ISMAR), Largo Fiera della Pesca, 2, 60125, Ancona, Italy.
[Ti] Título:Comparison of Lindane and Carbaryl Pesticide Bioaccumulation in the Common Sole (Solea solea).
[So] Source:Bull Environ Contam Toxicol;98(5):656-661, 2017 May.
[Is] ISSN:1432-0800
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Organochlorines and carbamates are common pesticides predominantly employed in agriculture. Large amounts of pesticides make their way into rivers and marine habitats. They accumulate in aquatic organisms through different exposure routes and gradually move up the food chain. Since contaminant bioaccumulation in animals is affected by several factors, this work harnessed several different approaches to explore the persistence of lindane, a long banned organochlorine pesticide, and carbaryl, a newer generation pesticide, in common sole (Solea solea), a major commercial species in Adriatic fisheries. Lindane was not only more accumulated than carbaryl in sole liver, but it was also detected in greater amount in muscle tissue, the edible part (lindane, 7 ± 4 ng/g; carbaryl, <0.004 ng/g w/w). Additional assays documented a greater accumulation of lindane in adults compared with juveniles and in specimens caught offshore than in those collected close to the coast. The present findings demonstrate the different accumulation dynamics of the two pesticides to confirm the benefits derived from the replacement of organochlorine pesticides with carbamate compounds.
[Mh] Termos MeSH primário: Carbaril/farmacocinética
Linguados/metabolismo
Lindano/farmacocinética
Praguicidas/farmacocinética
Poluentes Químicos da Água/farmacocinética
[Mh] Termos MeSH secundário: Animais
Carbaril/análise
Pesqueiros
Cadeia Alimentar
Hidrocarbonetos Clorados/análise
Hidrocarbonetos Clorados/farmacocinética
Lindano/análise
Fígado/efeitos dos fármacos
Fígado/metabolismo
Músculo Esquelético/efeitos dos fármacos
Músculo Esquelético/metabolismo
Praguicidas/análise
Rios/química
Alimentos Marinhos/análise
Poluentes Químicos da Água/análise
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Hydrocarbons, Chlorinated); 0 (Pesticides); 0 (Water Pollutants, Chemical); 59NEE7PCAB (Lindane); R890C8J3N1 (Carbaryl)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170609
[Lr] Data última revisão:
170609
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170315
[St] Status:MEDLINE
[do] DOI:10.1007/s00128-017-2056-z


  3 / 1149 MEDLINE  
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[PMID]:28259584
[Au] Autor:Oziolor EM; Howard W; Lavado R; Matson CW
[Ad] Endereço:Department of Environmental Science, Baylor University, Waco, TX, 76798, USA; Center for Reservoir and Aquatic Systems Research, Institute for Biomedical Studies, Baylor University, Waco, TX, 76798, USA. Electronic address: elias_oziolor@baylor.edu.
[Ti] Título:Induced pesticide tolerance results from detoxification pathway priming.
[So] Source:Environ Pollut;224:615-621, 2017 May.
[Is] ISSN:1873-6424
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Few studies in developmental toxicology have focused on whether early life contaminant exposure affects future susceptibility. Investigations in frogs suggested that early life exposure to a pesticide resulted in higher tolerance to a subsequent challenge. This led to the hypothesis that early-life stage exposures can alter phenotypically plastic traits during development, resulting in induced tolerance. Here, we used Gulf killifish (Fundulus grandis) to test the role of detoxification pathway priming in this inducible tolerance. In frogs, the induced tolerance is present five days after the end of the pre-exposure, but absent after a month. We show that a pre-exposure early in life with carbaryl, induces the activity of cytochrome P450 1A (CYP1A) and increases the ability of pre-exposed groups to metabolize carbaryl, likely because of activation of the aryl hydrocarbon receptor (AHR) pathway. Embryos pre-exposed to carbaryl had a 350-500% increase in CYP1A activity, threefold greater capacity to metabolize carbaryl and were more tolerant to a lethal challenge five days after the end of pre-exposure. However, ten days later the differences in CYP1A activity, metabolic capacity and tolerance between pre-exposed and control groups were no longer present. Thus, we conclude that the increase in tolerance observed in pre-exposed fish embryos was due to the activation of the AHR and other metabolic pathways, resulting in a prolonged increase in biotransformation capacity. This allowed individuals to more efficiently deal with subsequent chemical challenges for a short period after the initial pre-exposure. However, this induced tolerance was only short-lived due to the recycling of biotransformation enzymes in the cells as part of general cellular protein maintenance. These findings suggest that induced tolerance was likely due to induction of defense mechanisms during the duration of response to the original stressor, rather than a more permanent change in their ability to respond to future challenges.
[Mh] Termos MeSH primário: Carbaril/toxicidade
Fundulidae/metabolismo
Estágios do Ciclo de Vida/efeitos dos fármacos
Praguicidas/metabolismo
Poluentes Químicos da Água/metabolismo
Poluentes Químicos da Água/toxicidade
[Mh] Termos MeSH secundário: Animais
Texas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Pesticides); 0 (Water Pollutants, Chemical); R890C8J3N1 (Carbaryl)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170522
[Lr] Data última revisão:
170522
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170306
[St] Status:MEDLINE


  4 / 1149 MEDLINE  
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[PMID]:28080039
[Au] Autor:Alsammarraie FK; Lin M
[Ad] Endereço:Food Science Program, Division of Food System & Bioengineering, University of Missouri , Columbia, Missouri 65211-5160, United States.
[Ti] Título:Using Standing Gold Nanorod Arrays as Surface-Enhanced Raman Spectroscopy (SERS) Substrates for Detection of Carbaryl Residues in Fruit Juice and Milk.
[So] Source:J Agric Food Chem;65(3):666-674, 2017 Jan 25.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In recent years, there have been increasing concerns about pesticide residues in various foods. On the other hand, there is growing attention in utilizing novel nanomaterials as highly sensitive, low-cost, and reproducible substrates for surface-enhanced Raman spectroscopy (SERS) applications. The objective of this study was to develop a SERS method for the rapid detection of pesticides that were extracted from different types of food samples (fruit juice and milk). A new SERS substrate was prepared by assembling gold nanorods into standing arrays on a gold-coated silicon slide. The standing nanorod arrays were neatly arranged and were able to generate a strong electromagnetic field in SERS measurement. The as-prepared SERS substrate was utilized to detect carbaryl in acetonitrile/water solution, fruit juices (orange and grapefruit), and milk. The results show that the concentrations of carbaryl spiked in fruit juice and milk were linearly correlated with the concentrations predicted by the partial least-squares (PLS) models with r values of 0.91, 0.88, and 0.95 for orange juice, grapefruit juice, and milk, respectively. The SERS method was able to detect carbaryl that was extracted from fruit juice and milk samples at a 50 ppb level. The detection limits of carbaryl were 509, 617, and 391 ppb in orange juice, grapefruit juice, and milk, respectively. All detection limits are below the maximum residue limits that were set by the U.S. EPA. Moreover, satisfactory recoveries (82-97.5%) were accomplished for food samples using this method. These results demonstrate that SERS coupled with the standing gold nanorod array substrates is a rapid, reliable, sensitive, and reproducible method for the detection of pesticide residues in foods.
[Mh] Termos MeSH primário: Carbaril/análise
Contaminação de Alimentos/análise
Sucos de Frutas e Vegetais/análise
Ouro/química
Leite/química
Resíduos de Praguicidas/análise
Análise Espectral Raman/métodos
[Mh] Termos MeSH secundário: Animais
Bovinos
Nanotubos/química
Análise Espectral Raman/instrumentação
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Pesticide Residues); 7440-57-5 (Gold); R890C8J3N1 (Carbaryl)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170428
[Lr] Data última revisão:
170428
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170113
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.6b04774


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[PMID]:27988452
[Au] Autor:De Jesús Andino F; Lawrence BP; Robert J
[Ad] Endereço:Department of Microbiology and Immunology, University of Rochester Medical Center, Rochester, USA.
[Ti] Título:Long term effects of carbaryl exposure on antiviral immune responses in Xenopus laevis.
[So] Source:Chemosphere;170:169-175, 2017 Mar.
[Is] ISSN:1879-1298
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Water pollutants associated with agriculture may contribute to the increased prevalence of infectious diseases caused by ranaviruses. We have established the amphibian Xenopus laevis and the ranavirus Frog Virus 3 (FV3) as a reliable experimental platform for evaluating the effects of common waterborne pollutants, such as the insecticide carbaryl. Following 3 weeks of exposure to 10 ppb carbaryl, X. laevis tadpoles exhibited a marked increase in mortality and accelerated development. Exposure at lower concentrations (0.1 and 1.0 ppb) was not toxic, but it impaired tadpole innate antiviral immune responses, as evidenced by significantly decreased TNF-α, IL-1ß, IFN-I, and IFN-III gene expression. The defect in IFN-I and IL-1ß gene expression levels persisted after metamorphosis in froglets, whereas only IFN-I gene expression in response to FV3 was attenuated when carbaryl exposure was performed at the adult stage. These findings suggest that the agriculture-associated carbaryl exposure at low but ecologically-relevant concentrations has the potential to induce long term alterations in host-pathogen interactions and antiviral immunity.
[Mh] Termos MeSH primário: Carbaril/toxicidade
Infecções por Vírus de DNA/imunologia
Imunidade Inata/efeitos dos fármacos
Larva/efeitos dos fármacos
Ranavirus/patogenicidade
Poluentes Químicos da Água/toxicidade
[Mh] Termos MeSH secundário: Animais
Infecções por Vírus de DNA/genética
Infecções por Vírus de DNA/virologia
Expressão Gênica/efeitos dos fármacos
Perfilação da Expressão Gênica
Imunidade Inata/imunologia
Larva/crescimento & desenvolvimento
Larva/imunologia
Metamorfose Biológica/efeitos dos fármacos
Metamorfose Biológica/imunologia
Fatores de Tempo
Fator de Necrose Tumoral alfa/genética
Xenopus laevis
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Tumor Necrosis Factor-alpha); 0 (Water Pollutants, Chemical); R890C8J3N1 (Carbaryl)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161219
[St] Status:MEDLINE


  6 / 1149 MEDLINE  
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[PMID]:27986996
[Au] Autor:Ludington TS; Moore PA
[Ad] Endereço:Laboratory for Sensory Ecology, Department of Biological Sciences, Bowling Green State University, Bowling Green, OH, 43403, USA.
[Ti] Título:The Degree of Impairment of Foraging in Crayfish (Orconectes virilis) due to Insecticide Exposure is Dependent upon Turbulence Dispersion.
[So] Source:Arch Environ Contam Toxicol;72(2):281-293, 2017 Feb.
[Is] ISSN:1432-0703
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:As toxicants move into aquatic systems, the concentration at any point in space or time is heavily influenced by the flow dynamics. The dispersion of these chemicals creates a toxicant concentration that fluctuates widely in time and is highly dependent on the spatial heterogeneity of turbulence. Despite this knowledge on the movement of toxicants in natural systems, most ecotoxicological studies use static exposure paradigms that ignore the spatio-temporal dynamics of toxicants in aquatic systems. Although recent studies have begun to use pulsed paradigms in an attempt to mimic natural conditions, the heterogeneity of real concentrations in natural systems rarely is considered for use in these tests. Thus, understanding how organisms are impaired by naturally distributed toxicants is relatively unknown. The purpose of this experiment was to determine how turbulent dispersion of a toxicant negatively impacts a behavioral task and if altering the nature of turbulence will change the negative impact of the toxicant. Crayfish were exposed to a turbulent plume of carbaryl, an insecticide, under two different turbulent conditions and two different spatial conditions. Turbulence was altered by placing an obstruction within the flow which mimics a natural obstruction in lentic systems. Crayfish were exposed to sublethal concentrations of carbaryl for 48 h under these different dynamic conditions. After toxicant exposure, crayfish foraging ability was measured in a flow-through Y maze. We hypothesized that crayfish exposed to the toxicant under more turbulent conditions would exhibit more detrimental responses due to the increased variation in chemical fluctuations. The fine-scale chemical distribution of the toxicant and the three-dimensional velocity profile were characterized for each of the turbulent conditions and each of the spatial locations. Analyses of these data showed that changes in turbulence or spatial location created a unique exposure condition. Particularly, significant variations in the rise time, intermittency, and slope of toxicant pulses were quantified, whereas average concentration of the peaks remained constant across locations. Deficits in the foraging ability of crayfish exposed under these dynamic conditions paralleled the differences quantified in parameters of the turbulent toxicant plume. Given these results, the concept of toxicant exposure needs revision and needs to incorporate the more temporally based measures of toxicant dispersion. In addition, static and pulsed exposure models do not duplicate natural exposure and may not reflect behavioral or physiological impairments that occur under more realistic exposure conditions.
[Mh] Termos MeSH primário: Astacoidea/efeitos dos fármacos
Carbaril/toxicidade
Comportamento Alimentar/efeitos dos fármacos
Inseticidas/toxicidade
Movimentos da Água
Poluentes Químicos da Água/toxicidade
[Mh] Termos MeSH secundário: Animais
Feminino
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Insecticides); 0 (Water Pollutants, Chemical); R890C8J3N1 (Carbaryl)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170207
[Lr] Data última revisão:
170207
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161218
[St] Status:MEDLINE
[do] DOI:10.1007/s00244-016-0341-1


  7 / 1149 MEDLINE  
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[PMID]:27940076
[Au] Autor:Zhang Y; Han L; He Q; Chen W; Sun C; Wang X; Chen X; Wang R; Hsiao CD; Liu K
[Ad] Endereço:Biology Institute of Shandong Academy of Sciences, Key Laboratory for Drug Screening Technology of Shandong Academy of Sciences, Shandong Provincial Engineering Laboratory for Biological Testing Technology, Key Laboratory for Biosensor of Shandong Province, 19 Keyuan Road, Lixia District, Jinan 2500
[Ti] Título:A rapid assessment for predicting drug-induced hepatotoxicity using zebrafish.
[So] Source:J Pharmacol Toxicol Methods;84:102-110, 2017 Mar - Apr.
[Is] ISSN:1873-488X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:INTRODUCTION: Zebrafish have been used as a model to access drug-induced hepatotoxicity. However, individual differences occur in the liver development of zebrafish. METHODS: We used a transgenic line of zebrafish that expressed enhanced green fluorescent protein (EGFP) in the liver and then used a calculation of the liver index area, a potentially new endpoint of hepatotoxicity, to evaluate drug-induced liver injury. To further validate the reliability of the liver area index as a quick evaluation of zebrafish liver function damage, the liver area index level was correlated with hepatic transaminase activities using the Pearson correlation coefficient and confirmed by histopathology. RESULTS: Zebrafish larvae treated with high doses of the known mammalian hepatotoxic drugs carbaryl, isoniazide, and pyrazinamide showed significantly decreased liver area index levels, which are suggestive of liver injury and correspond with the higher alanine transaminase (ALT) and aspartate transaminase (AST) activities and histological liver alterations. The results showed a significant negative correlation between the degree of liver injury and the liver area index level. DISCUSSION: Our data support the use of the liver area index as a reliable and comparable indicator to screen hepatotoxic agents using the zebrafish model.
[Mh] Termos MeSH primário: Doença Hepática Induzida por Substâncias e Drogas/metabolismo
Doença Hepática Induzida por Substâncias e Drogas/patologia
Fígado/efeitos dos fármacos
Fígado/metabolismo
[Mh] Termos MeSH secundário: Animais
Animais Geneticamente Modificados
Carbaril/toxicidade
Doença Hepática Induzida por Substâncias e Drogas/genética
Relação Dose-Resposta a Droga
Feminino
Proteínas de Fluorescência Verde/biossíntese
Proteínas de Fluorescência Verde/genética
Isoniazida/toxicidade
Fígado/patologia
Masculino
Valor Preditivo dos Testes
Fatores de Tempo
Peixe-Zebra
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (enhanced green fluorescent protein); 147336-22-9 (Green Fluorescent Proteins); R890C8J3N1 (Carbaryl); V83O1VOZ8L (Isoniazid)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170707
[Lr] Data última revisão:
170707
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161213
[St] Status:MEDLINE


  8 / 1149 MEDLINE  
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[PMID]:27939635
[Au] Autor:Pochini KM; Hoverman JT
[Ad] Endereço:Department of Forestry and Natural Resources, Purdue University, 715 West State St., West Lafayette, IN 47907-2061, United States. Electronic address: kpochini4@gmail.com.
[Ti] Título:Reciprocal effects of pesticides and pathogens on amphibian hosts: The importance of exposure order and timing.
[So] Source:Environ Pollut;221:359-366, 2017 Feb.
[Is] ISSN:1873-6424
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Ecological communities are increasingly exposed to natural and anthropogenic stressors. While the effects of individual stressors have been broadly investigated, there is growing evidence that multiple stressors are frequently encountered underscoring the need to examine interactive effects. Pesticides and infectious diseases are two common stressors that regularly occur together in nature. Given the documented lethal and sublethal effects of each stressor on individuals, there is the potential for interactive effects that alter disease outcomes and pesticide toxicity. Using larval wood frogs (Lithobates sylvaticus), we examined the reciprocal interaction between insecticides (carbaryl and thiamethoxam) and the viral pathogen ranavirus by testing whether: (1) prior ranavirus infection influences pesticide toxicity and (2) sublethal pesticide exposure increases susceptibility to and transmission of ranavirus. We found that prior infection with ranavirus increased pesticide toxicity; median lethal concentration (LC50) estimates were reduced by 72 and 55% for carbaryl and thiamethoxam, respectively. Importantly, LC50 estimates were reduced to concentrations found in natural systems. This is the first demonstration that an infection can alter pesticide toxicity. We also found that prior pesticide exposure exacerbated disease-induced mortality by increasing mortality rates, but effects on infection prevalence and transmission of the pathogen were minimal. Collectively, our results underscore the importance of incorporating complexity (i.e. order and timing of exposures) into research examining the interactions between natural and anthropogenic stressors. Given the environmental heterogeneity present in nature, such research will provide a more comprehensive understanding of how stressors affect wildlife.
[Mh] Termos MeSH primário: Exposição Ambiental/estatística & dados numéricos
Praguicidas/toxicidade
Ranidae/fisiologia
Poluentes Químicos da Água/toxicidade
[Mh] Termos MeSH secundário: Animais
Carbaril/toxicidade
Monitoramento Ambiental
Inseticidas/toxicidade
Larva/efeitos dos fármacos
Ranavirus
Ranidae/virologia
Estresse Fisiológico
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Insecticides); 0 (Pesticides); 0 (Water Pollutants, Chemical); R890C8J3N1 (Carbaryl)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161213
[St] Status:MEDLINE


  9 / 1149 MEDLINE  
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Barros, Silvia Berlanga de Moraes
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[PMID]:27829164
[Au] Autor:Ferrucio B; Tiago M; Fannin RD; Liu L; Gerrish K; Maria-Engler SS; Paules RS; Barros SB
[Ad] Endereço:Department of Clinical Chemistry and Toxicology, School of Pharmaceutical Sciences, University of São Paulo, São Paulo, SP, Brazil, 05508-000. Electronic address: biferrucio@usp.br.
[Ti] Título:Molecular effects of 1-naphthyl-methylcarbamate and solar radiation exposures on human melanocytes.
[So] Source:Toxicol In Vitro;38:67-76, 2017 Feb.
[Is] ISSN:1879-3177
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Carbaryl (1-naphthyl-methylcarbamate), a broad-spectrum insecticide, has recently been associated with the development of cutaneous melanoma in an epidemiological cohort study with U.S. farm workers also exposed to ultraviolet radiation, the main etiologic factor for skin carcinogenesis. We hypothesized that carbaryl exposure may increase deleterious effects of UV solar radiation on skin melanocytes. This study aimed to characterize human melanocytes after individual or combined exposure to carbaryl (100µM) and solar radiation (375mJ/cm ). In a microarray analysis, carbaryl, but not solar radiation, induced an oxidative stress response, evidenced by the upregulation of antioxidant genes, such as Hemeoxygenase-1 (HMOX1), and downregulation of Microphtalmia-associated Transcription Factor (MITF), the main regulator of melanocytic activity; results were confirmed by qRT-PCR. Carbaryl and solar radiation induced a gene response suggestive of DNA damage and cell cycle alteration. The expression of CDKN1A, BRCA1/2 and MDM2 genes was notably more intense in the combined treatment group, in a synergistic manner. Flow cytometry assays demonstrated S-phase cell cycle arrest, reduced apoptosis levels and faster induction of cyclobutane pyrimidine dimers (CPD) lesions in carbaryl treated groups. Our data suggests that carbaryl is genotoxic to human melanocytes, especially when associated with solar radiation.
[Mh] Termos MeSH primário: Carbaril/toxicidade
Melanócitos/efeitos dos fármacos
Melanócitos/efeitos da radiação
Raios Ultravioleta/efeitos adversos
[Mh] Termos MeSH secundário: Apoptose/efeitos dos fármacos
Apoptose/efeitos da radiação
Ciclo Celular/efeitos dos fármacos
Ciclo Celular/efeitos da radiação
Divisão Celular/efeitos dos fármacos
Divisão Celular/efeitos da radiação
Sobrevivência Celular/efeitos dos fármacos
Sobrevivência Celular/efeitos da radiação
Células Cultivadas
Criança
Pré-Escolar
Dano ao DNA
Estresse do Retículo Endoplasmático/efeitos dos fármacos
Estresse do Retículo Endoplasmático/efeitos da radiação
Seres Humanos
Masculino
Estresse Oxidativo/efeitos dos fármacos
Estresse Oxidativo/efeitos da radiação
Luz Solar
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
R890C8J3N1 (Carbaryl)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170330
[Lr] Data última revisão:
170330
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161110
[St] Status:MEDLINE


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[PMID]:27296064
[Au] Autor:Prakash Krishnan Muthaiah V; Ding D; Salvi R; Roth JA
[Ad] Endereço:Center for Hearing and Deafness, University at Buffalo, Buffalo, New York, 14214.
[Ti] Título:Carbaryl-induced ototoxicity in rat postnatal cochlear organotypic cultures.
[So] Source:Environ Toxicol;32(3):956-969, 2017 Mar.
[Is] ISSN:1522-7278
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Carbaryl, a widely used carbamate-based insecticide, is a potent anticholinesterase known to induce delayed neurotoxicity following chronic exposure. However, its potential toxic effects on the cochlea, the sensory organ for hearing that contains cholinergic efferent neurons and acetylcholine receptors on the hair cells (HC) and spiral ganglion neurons has heretofore not been evaluated. To assess ototoxic potential of carbaryl, cochlear organotypic cultures from postnatal day 3 rats were treated with doses of carbaryl ranging from 50 to 500 µM for 48 h up to 96 h. Carbaryl damaged both the sensory HC and spiral ganglion neurons in a dose- and duration-dependent manner. HC and neuronal damage was observed at carbaryl concentrations as low as 50 µM after 96-h treatment and 100 µM after 48-h treatment. Hair cell was greatest in the high frequency basal region of the cochlea and progressively decreased towards the apex consistent with the majority of ototoxic drugs. In contrast, damage to the spiral ganglion neurons was of similar magnitude in the basal and apical regions of the cochlea. Carbaryl damage was characterized by soma shrinkage, nuclear condensation and fragmentation, and blebbing, morphological features of programmed cell death. Carbaryl upregulated the expression of executioner caspase-3 in HC and spiral ganglion neurons indicating that cellular damage occurred primarily by caspase-mediated apoptosis. These results suggest that chronic exposure to carbaryl and other carbamate anticholinesterases may be ototoxic. © 2016 Wiley Periodicals, Inc. Environ Toxicol 32: 956-969, 2017.
[Mh] Termos MeSH primário: Apoptose/efeitos dos fármacos
Carbaril/toxicidade
Cóclea/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Caspase 3/metabolismo
Células Cultivadas
Cóclea/metabolismo
Cóclea/patologia
Células Ciliadas Auditivas/citologia
Células Ciliadas Auditivas/efeitos dos fármacos
Células Ciliadas Auditivas/metabolismo
Microscopia Confocal
Ratos
Ratos Sprague-Dawley
Gânglio Espiral da Cóclea/efeitos dos fármacos
Gânglio Espiral da Cóclea/metabolismo
Gânglio Espiral da Cóclea/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
EC 3.4.22.- (Caspase 3); R890C8J3N1 (Carbaryl)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170406
[Lr] Data última revisão:
170406
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160615
[St] Status:MEDLINE
[do] DOI:10.1002/tox.22296



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