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[PMID]: 27773359 [Au] Autor: van Wendel de Joode B; Mora AM; Lindh CH; Hernández-Bonilla D; Córdoba L; Wesseling C; Hoppin JA; Mergler D [Ad] Endereço: Central American Institute for Studies on Toxic Substances (IRET), Universidad Nacional, Heredia, Costa Rica. Electronic address: bernavanwendel@una.cr. [Ti] Título: Pesticide exposure and neurodevelopment in children aged 6-9 years from Talamanca, Costa Rica. [So] Source: Cortex;85:137-150, 2016 12. [Is] ISSN: 1973-8102 [Cp] País de publicação: Italy [La] Idioma: eng [Ab] Resumo: Certain pesticides may affect children's neurodevelopment. We assessed whether pesticide exposure was associated with impaired neurobehavioral outcomes in children aged 6-9 years. We conducted a cross-sectional study in 140 children living near banana plantations and plantain farms in the Talamanca County, Costa Rica and assessed their neurobehavioral performance. Exposure was determined by analyzing urinary metabolites of chlorpyrifos (3,5,6-trichloro-2-pyridinol, TCPy), mancozeb (ethylenethiourea, ETU), and pyrethroids (3-phenoxybenzoic acid, 3-PBA). Repeated urine samples were obtained for 36 children. We estimated associations of pesticide concentrations with neurobehavioral outcomes using multivariable linear and logistic regression models. Median (25th-75th percentiles) TCPy, ETU, and 3-PBA concentrations were 1.4 (.7-3.1), 1.2 (.7-3.0), and .8 (.5-1.5) µg/L, respectively. Intraclass correlation coefficients (ICC) ranged between .32 and .67. After adjustment for potential confounders, higher urinary TCPy concentrations were associated with poorer working memory in boys (n = 59) (ß per 10-fold increase in TCPy concentrations = -7.5, 95% CI: -14.4, -.7); poorer visual motor coordination (ß = -1.4, 95% CI: -2.7, -.1); increased prevalence of parent-reported cognitive problems/inattention (adjusted OR per 10-fold increase in urinary concentrations = 5.8, 95% CI: 1.6, 22.9), oppositional disorders (aOR = 3.9, 95% CI: 1.0, 16.0), and ADHD (aOR = 6.8, 95% CI: 1.8, 28.6), and; decreased ability to discriminate colors (aOR = 6.6, 95% CI: 1.6, 30.3; the higher the score the worse). Higher ETU concentrations were associated with poorer verbal learning outcomes (ß = -7.0, 95% CI: -12.7, -1.3). Higher 3-PBA concentrations were associated with poorer processing speed scores, particularly in girls (ß = -8.8, 95% CI: -16.1, -1.4). Our findings indicate that children living near banana and plantain plantations are exposed to pesticides that may affect their neurodevelopment, which for certain domains may differ between boys and girls. We recommend the implementation of measures to reduce pesticide exposure in children living nearby banana plantations. [Mh] Termos MeSH primário: Benzoatos/urina Sistema Nervoso Central/efeitos dos fármacos Sistema Nervoso Central/crescimento & desenvolvimento Clorpirifos/urina Maneb/urina Praguicidas/urina Zineb/urina [Mh] Termos MeSH secundário: Criança Comportamento Infantil Costa Rica Estudos Transversais Feminino Seres Humanos Masculino Piridonas/urina [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Benzoates); 0 (Pesticides); 0 (Pyridones); 12427-38-2 (Maneb); 6515-38-4 (3,5,6-trichloro-2-pyridinol); 69DC2655VH (3-phenoxybenzoic acid); JCS58I644W (Chlorpyrifos); R0HY55EB9E (mancozeb); X1FSB1OZPT (Zineb) [Em] Mês de entrada: 1711 [Cu] Atualização por classe: 171213 [Lr] Data última revisão: 171213 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161025 [St] Status: MEDLINE

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[PMID]: 28330765 [Au] Autor: Zizza M; Di Lorenzo M; Laforgia V; Furia E; Sindona G; Canonaco M; Facciolo RM [Ad] Endereço: Comparative Neuroanatomy Laboratory, Biology, Ecology and Earth Science Department (DiBEST), University of Calabria, Arcavacata of Rende, 87036, CS, Italy. [Ti] Título: HSP90 and pCREB alterations are linked to mancozeb-dependent behavioral and neurodegenerative effects in a marine teleost. [So] Source: Toxicol Appl Pharmacol;323:26-35, 2017 May 15. [Is] ISSN: 1096-0333 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: The pesticide mancozeb (mz) is recognized as a potent inducer of oxidative stress due to its ability to catalyze the production of reactive oxygen species plus inhibiting mitochondrial respiration thus becoming an environmental risk for neurodegenerative diseases. Despite numerous toxicological studies on mz have been directed to mammals, attention on marine fish is still lacking. Thus, it was our intention to evaluate neurobehavioral activities of ornate wrasses (Thalassoma pavo) exposed to 0.2mg/l of mz after a preliminary screening test (0.07-0.3mg/l). Treated fish exhibited an evident (p<0.001) latency to reach T-maze arms (>1000%) while exploratory attitudes (total arm entries) diminished (-50%; p<0.05) versus controls during spontaneous exploration tests. Moreover, they showed evident enhancements (+111%) of immobility in the cylinder test. Contextually, strong (-88%; p<0.01) reductions of permanence in light zone of the Light/Dark apparatus along with diminished crossings (-65%) were also detected. Conversely, wrasses displayed evident enhancements (160%) of risk assessment consisting of fast entries in the dark side of this apparatus. From a molecular point of view, a notable activation (p<0.005) of the brain transcription factor pCREB occurred during mz-exposure. Similarly, in situ hybridization supplied increased HSP90 mRNAs in most brain areas such as the lateral part of the dorsal telencephalon (Dl; +68%) and valvula of the cerebellum (VCe; +35%) that also revealed evident argyrophilic signals. Overall, these first indications suggest a possible protective role of the early biomarkers pCREB and HSP90 against fish toxicity. [Mh] Termos MeSH primário: Comportamento Animal/efeitos dos fármacos Encéfalo/efeitos dos fármacos Proteína de Ligação ao Elemento de Resposta ao AMP Cíclico/metabolismo Proteínas de Peixes/metabolismo Peixes/metabolismo Fungicidas Industriais/toxicidade Proteínas de Choque Térmico HSP90/metabolismo Maneb/toxicidade Degeneração Neural Síndromes Neurotóxicas/etiologia Poluentes Químicos da Água/toxicidade Zineb/toxicidade [Mh] Termos MeSH secundário: Animais Encéfalo/metabolismo Encéfalo/patologia Encéfalo/fisiopatologia Comportamento Exploratório/efeitos dos fármacos Feminino Proteínas de Peixes/genética Peixes/genética Proteínas de Choque Térmico HSP90/genética Atividade Motora/efeitos dos fármacos Síndromes Neurotóxicas/metabolismo Síndromes Neurotóxicas/patologia Síndromes Neurotóxicas/psicologia Tempo de Reação/efeitos dos fármacos Fatores de Tempo [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Cyclic AMP Response Element-Binding Protein); 0 (Fish Proteins); 0 (Fungicides, Industrial); 0 (HSP90 Heat-Shock Proteins); 0 (Water Pollutants, Chemical); 12427-38-2 (Maneb); R0HY55EB9E (mancozeb); X1FSB1OZPT (Zineb) [Em] Mês de entrada: 1704 [Cu] Atualização por classe: 170424 [Lr] Data última revisão: 170424 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170324 [St] Status: MEDLINE

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[PMID]: 28110212 [Au] Autor: López-Fernández O; Pose-Juan E; Rial-Otero R; Simal-Gándara J [Ad] Endereço: Nutrition and Bromatology Group, Analytical and Food Chemistry Department. Faculty of Food Science and Technology, University of Vigo, Ourense Campus, E-32004 Ourense, Spain. [Ti] Título: Effects of hydrochemistry variables on the half-life of mancozeb and on the hazard index associated to the sum of mancozeb and ethylenethiourea. [So] Source: Environ Res;154:253-260, 2017 04. [Is] ISSN: 1096-0953 [Cp] País de publicação: Netherlands [La] Idioma: eng [Ab] Resumo: Mancozeb is a dithiocarbamate non-systemic agricultural fungicide with multi-site, protective action. It helps to control many fungal diseases in a wide range of field crops, fruits, nuts, vegetables, and ornamental plants. We have investigated the stability profiles of mancozeb in aqueous solutions to determine the effect of pH, temperature and light on the degradation process of mancozeb. In addition, the toxicological risk for humans associated with the joint intake of mancoze7b and its final degradation product, ethylenethiourea (ETU), was calculated and modelled as a function of the experimental conditions. Stability study results showed a very low stability profile of mancozeb in all the aqueous solutions with rapid degradation that varied with experimental conditions. The process followed first order kinetics. The study of the degradation kinetics showed a significant effect of pH*temperature interaction on the degradation process. The results also expressed that light has a greater impact on the stability of mancozeb and the formation of ETU. The current study concludes that mancozeb is unstable in aqueous solutions, particularly at an acid pH, in addition to presenting both severe light and lower temperature sensitivity. The toxicological risk associated with mancozeb degradation increases with time and temperature, being higher at basic pH and in absence of light. [Mh] Termos MeSH primário: Etilenotioureia/química Etilenotioureia/metabolismo Fungicidas Industriais/química Fungicidas Industriais/metabolismo Maneb/química Maneb/metabolismo Zineb/química Zineb/metabolismo [Mh] Termos MeSH secundário: Meia-Vida Concentração de Íons de Hidrogênio Hidrólise Cinética Luz Fotólise Temperatura Ambiente Fatores de Tempo Água [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Fungicides, Industrial); 059QF0KO0R (Water); 12427-38-2 (Maneb); 24FOJ4N18S (Ethylenethiourea); R0HY55EB9E (mancozeb); X1FSB1OZPT (Zineb) [Em] Mês de entrada: 1705 [Cu] Atualização por classe: 171119 [Lr] Data última revisão: 171119 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170123 [St] Status: MEDLINE

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[PMID]: 28073049 [Au] Autor: Medda E; Santini F; De Angelis S; Franzellin F; Fiumalbi C; Perico A; Gilardi E; Mechi MT; Marsili A; Citroni A; Leandri A; Mantovani A; Vitti P; Olivieri A [Ad] Endereço: National Centre for Epidemiology Surveillance and Health Promotion, Italian National Institute of Health, Viale Regina Elena 299, 00161 Rome, Italy. [Ti] Título: Iodine nutritional status and thyroid effects of exposure to ethylenebisdithiocarbamates. [So] Source: Environ Res;154:152-159, 2017 04. [Is] ISSN: 1096-0953 [Cp] País de publicação: Netherlands [La] Idioma: eng [Ab] Resumo: INTRODUCTION: Italy is still characterized by a mild iodine deficiency and is among the most intensive users of chemical products for agriculture in Europe. The aim of this study was i) to evaluate thyroid effects of exposure to mancozeb, a fungicide widely used in agriculture, in a sample of Italian grapevine workers, and ii) to verify whether the iodine intake may modulate the risk of thyroid disruption due to the mancozeb metabolite ethylenthiourea (ETU). METHODS: One hundred seventy-seven occupationally exposed male workers (29 from Chianti, a mild iodine deficient area, and 148 from Bolzano an iodine sufficient province) and 74 non-occupationally exposed male controls (34 from Chianti and 40 from Bolzano) were enrolled in the study. Serum biomarkers of thyroid function, as well as urinary iodine and ETU concentrations were assessed. Moreover all the recruited subjects underwent clinical examination and thyroid ultrasound. RESULTS: Multivariate comparisons showed lower mean serum levels of FT4 in Chianti-workers as compared to Bolzano-workers. Moreover, an increased urinary iodine excretion (>250µg/L) was more frequently found among more exposed workers (ETU>20µg/L) than among less exposed ones and this effect was more pronounced in Chianti- than in Bolzano-workers. Chianti-workers also showed a significantly higher frequency of very low thyroid volume (≤6.0ml) as compared to controls. CONCLUSIONS: These findings showed a mild thyroid disrupting effect due to occupational exposure to mancozeb, more pronounced in workers residing in an area characterized by a mild to moderate iodine deficiency as compared to workers residing in an area covered by a long-lasting iodine prophylaxis program. [Mh] Termos MeSH primário: Fungicidas Industriais/toxicidade Iodo/administração & dosagem Maneb/toxicidade Doenças da Glândula Tireoide/prevenção & controle Zineb/toxicidade [Mh] Termos MeSH secundário: Adulto Idoso Doenças dos Trabalhadores Agrícolas/induzido quimicamente Doenças dos Trabalhadores Agrícolas/prevenção & controle Estudos de Casos e Controles Etilenotioureia/análise Fazendeiros Seres Humanos Iodo/deficiência Itália Masculino Meia-Idade Estado Nutricional Exposição Ocupacional/efeitos adversos Doenças da Glândula Tireoide/induzido quimicamente Testes de Função Tireóidea [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Fungicides, Industrial); 12427-38-2 (Maneb); 24FOJ4N18S (Ethylenethiourea); 9679TC07X4 (Iodine); R0HY55EB9E (mancozeb); X1FSB1OZPT (Zineb) [Em] Mês de entrada: 1705 [Cu] Atualização por classe: 171119 [Lr] Data última revisão: 171119 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170111 [St] Status: MEDLINE

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[PMID]: 27742361 [Au] Autor: Marques A; Rego A; Guilherme S; Gaivão I; Santos MA; Pacheco M [Ad] Endereço: Department of Biology and CESAM, University of Aveiro, 3810-193 Aveiro, Portugal. Electronic address: anammarques@ua.pt. [Ti] Título: Evidences of DNA and chromosomal damage induced by the mancozeb-based fungicide Mancozan in fish (Anguilla anguilla L.). [So] Source: Pestic Biochem Physiol;133:52-58, 2016 Oct. [Is] ISSN: 1095-9939 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: The formulation Mancozan , containing mancozeb as active ingredient, is among the most widely used fungicides. Although mancozeb has been detected in surface waters, studies addressing the genotoxic risk to fish arising from the use of this formulation, testing environmentally realistic concentrations, are absent from the literature. Hence, this work aimed to investigate the DNA and chromosome damaging potential of Mancozan (0.29 and 2.9µgL ) in the European eel (Anguilla anguilla L.), after a short-term exposure (3days), through the adoption of the comet and the erythrocytic nuclear abnormality (ENA) assays. In addition, it was intended to elucidate the subjacent damage mechanisms, improving the comet assay with the adoption of the endonucleases formamidopyrimidine DNA glycosylase (FPG) and endonuclease III (EndoIII), which detect oxidized bases. The highest Mancozan concentration was able to affect the DNA integrity (comet assay), while the adoption of endonucleases pointed out an oxidative cause to the damage. Regarding the chromosomal damage (ENA assay), both concentrations displayed significant effects, revealing the clastogenic and/or aneugenic properties of Mancozan . Furthermore, the two genotoxic endpoints were significantly correlated. Overall, the results revealed a genetic hazard to fish inhabiting aquatic systems contaminated by Mancozan and strongly recommend the development of biomonitoring and regulatory policies regarding the utilization of this agrochemical. [Mh] Termos MeSH primário: Anguilla/genética Dano ao DNA Fungicidas Industriais/toxicidade Maneb/toxicidade Poluentes Químicos da Água/toxicidade Zineb/toxicidade [Mh] Termos MeSH secundário: Animais Cromossomos/efeitos dos fármacos Ensaio Cometa DNA-Formamidopirimidina Glicosilase/metabolismo Endodesoxirribonucleases/metabolismo Eritrócitos/efeitos dos fármacos [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Fungicides, Industrial); 0 (Water Pollutants, Chemical); 12427-38-2 (Maneb); EC 3.1.- (Endodeoxyribonucleases); EC 3.2.2.23 (DNA-Formamidopyrimidine Glycosylase); R0HY55EB9E (mancozeb); X1FSB1OZPT (Zineb) [Em] Mês de entrada: 1703 [Cu] Atualização por classe: 170313 [Lr] Data última revisão: 170313 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161016 [St] Status: MEDLINE

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[PMID]: 27715504 [Au] Autor: Majumder S; Shakil NA; Kumar J; Banerjee T; Sinha P; Singh BB; Garg P [Ad] Endereço: a Division of Agricultural Chemicals, Indian Agricultural Research Institute, ICAR , New Delhi , India. [Ti] Título: Eco-friendly PEG-based controlled release nano-formulations of Mancozeb: Synthesis and bioefficacy evaluation against phytopathogenic fungi Alternaria solani and Sclerotium rolfsii. [So] Source: J Environ Sci Health B;51(12):873-880, 2016 Dec. [Is] ISSN: 1532-4109 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: Controlled release (CR) nano-formulations of Mancozeb (manganese-zinc double salt of N,N-bisdithiocarbamic acid), a protective fungicide, have been prepared using laboratory-synthesized poly(ethylene glycols) (PEGs)-based functionalized amphiphilic copolymers without using any surfactants or external additives. The release kinetics of the developed Mancozeb CR formulations were studied and compared with that of commercially available 42% suspension concentrate and 75% wettable powder. Maximum amount of Mancozeb was released on 42nd day for PEG-600 and octyl chain, PEG-1000 and octyl chain, and PEG-600 and hexadecyl chain, on 35th day for PEG-1000 and hexadecyl chain, on 28th day for PEG-1500 and octyl chain, PEG-2000 and octyl chain, PEG-1500 and hexadecyl chain, and PEG-2000 and hexadecyl chain in comparison to both commercial formulations (15th day). The diffusion exponent (n value) of Mancozeb in water ranged from 0.42 to 0.62 in tested formulations. The half-release (t ) values ranged from 17.35 to 35.14 days, and the period of optimum availability of Mancozeb ranged from 18.54 to 35.42 days. Further, the in vitro bioefficacy evaluation of developed formulations was done against plant pathogenic fungi Alternaria solani and Sclerotium rolfsii by poison food technique. Effective dose for 50% inhibition in mgL (ED ) values of developed formulations varied from 1.31 to 2.79 mg L for A. solani, and 1.60 to 3.14 mg L for S. rolfsii. The present methodology is simple, economical, and eco-friendly for the development of environment-friendly CR formulations of Mancozeb. These formulations can be used to optimize the release of Mancozeb to achieve disease control for the desired period depending upon the matrix of the polymer used. Importantly, the maximum amount of active ingredient remains available for a reasonable period after application. In addition, the developed CR formulations were found to be suitable for fungicidal applications, allowing use of Mancozeb in lower doses. [Mh] Termos MeSH primário: Alternaria/efeitos dos fármacos Basidiomycota/efeitos dos fármacos Fungicidas Industriais/farmacologia Maneb/síntese química Maneb/farmacologia Zineb/síntese química Zineb/farmacologia [Mh] Termos MeSH secundário: Alternaria/patogenicidade Basidiomycota/patogenicidade Técnicas de Química Sintética Preparações de Ação Retardada Difusão Fungicidas Industriais/administração & dosagem Fungicidas Industriais/síntese química Cinética Maneb/administração & dosagem Nanocompostos/administração & dosagem Nanocompostos/química Polietilenoglicóis/química Água/química Zineb/administração & dosagem [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Delayed-Action Preparations); 0 (Fungicides, Industrial); 059QF0KO0R (Water); 12427-38-2 (Maneb); 30IQX730WE (Polyethylene Glycols); R0HY55EB9E (mancozeb); X1FSB1OZPT (Zineb) [Em] Mês de entrada: 1704 [Cu] Atualização por classe: 170817 [Lr] Data última revisão: 170817 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161008 [St] Status: MEDLINE

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[PMID]: 27327564 [Au] Autor: Bhat SA; Bhat WF; Bano B [Ad] Endereço: Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh-202002, India. bilqeesbanos@gmail.com. [Ti] Título: Spectroscopic evaluation of the interaction between pesticides and chickpea cystatin: comparative binding and toxicity analyses. [So] Source: Environ Sci Process Impacts;18(7):872-81, 2016 Jul 13. [Is] ISSN: 2050-7895 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: The binding study of pesticides with proteins is of great importance in ecotoxicology. In this study, a comparative interaction mechanism of phytocystatin with three pesticides has been presented, each from a different class-glyphosate herbicide (GPS), chlorpyrifos insecticide (CPF), and mancozeb fungicide (MCZ). The interaction of purified chickpea cystatin (CPC) has been characterized by fluorescence, UV, and circular dichroism (CD) spectroscopic methods. The study revealed association constants (Ka) of 52 M(-1), 1.145 × 10(3) M(-1), and 36.12 M(-1) for the interaction of CPF, MCZ, and GPS with CPC, respectively, signifying the high affinity interaction for MCZ. Structural changes (at tertiary and secondary levels) were confirmed by UV-visible, intrinsic fluorescence and CD spectroscopy. The results showed that the effect on the CPC structure was more pronounced in the case of MCZ, which was followed by CPF and then GPS. The functional analysis of the pesticide treated inhibitor showed a decline in antipapain activity which varied with the time and dose as well as the class of pesticide. MCZ was relatively much more toxic as compared to CPF and GPS. Reactive oxygen species responsible for inhibitor damage were also analyzed. The results obtained implicate that the exposure of plants to pesticides may lead to physicochemical changes in proteins such as phytocystatins leading to physiological damage to the plant system. [Mh] Termos MeSH primário: Clorpirifos/química Cicer/química Cistatinas/química Glicina/análogos & derivados Maneb/química Praguicidas/química Espécies Reativas de Oxigênio/química Zineb/química [Mh] Termos MeSH secundário: Animais Clorpirifos/toxicidade Glicina/química Glicina/toxicidade Maneb/toxicidade Praguicidas/toxicidade Zineb/toxicidade [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Cystatins); 0 (Pesticides); 0 (Reactive Oxygen Species); 12427-38-2 (Maneb); 4632WW1X5A (glyphosate); JCS58I644W (Chlorpyrifos); R0HY55EB9E (mancozeb); TE7660XO1C (Glycine); X1FSB1OZPT (Zineb) [Em] Mês de entrada: 1703 [Cu] Atualização por classe: 170817 [Lr] Data última revisão: 170817 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160622 [St] Status: MEDLINE [do] DOI: 10.1039/c6em00195e

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[PMID]: 27235710 [Au] Autor: Srivastava AK; Mishra S; Ali W; Shukla Y [Ad] Endereço: Proteomics & Environmental Carcinogenesis Laboratory, Food, Drug and Chemical Toxicology Group, CSIR-Indian Institute of Toxicology Research, Vishvigyan Bhavan, 31, Mahatma Gandhi Marg, Lucknow-226001, Uttar Pradesh, India. [Ti] Título: Protective effects of lupeol against mancozeb-induced genotoxicity in cultured human lymphocytes. [So] Source: Phytomedicine;23(7):714-24, 2016 Jun 15. [Is] ISSN: 1618-095X [Cp] País de publicação: Germany [La] Idioma: eng [Ab] Resumo: BACKGROUND: Lup-20(29)-en-3H-ol (Lupeol), a dietary pentacyclic triterpenoid has been shown to possess multiple medicinal activities including anti-inflammatory, anti-oxidant and anti-carcinogenic effects. Mancozeb is a widely used broad-spectrum fungicide with well-known carcinogenic hazards in rodents. PURPOSE: The present study has been designed to investigate the protective effects of lupeol against mancozeb-induced genotoxicity and apoptosis in cultured human lymphocytes (CHLs). METHODS: The genotoxic effect of mancozeb was evaluated by chromosomal aberration and micronucleus assays. The cell cycle kinetics and intracellular reactive oxygen species (ROS) generation was measured by flow cytometry. The levels of anti-oxidant enzymes and lipid peroxidation (LPO) were estimated by enzymatic assays. The localization of p65NF-κB was measured by immunocytochemical analysis. The differential expression of genes associated with genotoxicity was measured by qRT-PCR. RESULTS: Mancozeb exposure (5µg/ml) for 24h caused significant induction of chromosomal aberrations (CAs) and micronuclei (MN) formation in CHLs. Pre-and post-treatment (25 and 50µg/ml) of lupeol for 24h significantly (p<0.05) reduced the frequency of CAs and MN induction, in a dose-dependent manner in mancozeb treated CHLs. Concomitantly, lupeol pre-treatment for 24h significantly increased the levels of anti-oxidant enzymes, superoxide dismutase (SOD) and catalase and decreased ROS generation and LPO. Additionally, lupeol pre-treatment significantly reduced mancozeb-induced apoptosis as shown by Sub-G1 peak analysis and annexin V-PI assay, in a dose dependent manner. Moreover, pre-treatment with lupeol attenuated mancozeb-induced NF-κB activation in CHLs. Furthermore, the results of qRT-PCR showed that lupeol pre-treatment significantly (p<0.05) decreased mancozeb-induced expression of DNA damage (p53, MDM2, COX-2, GADD45α and p21) and increased expression of DNA repair responsive genes (hOGG1 and XRCC1) in CHLs. CONCLUSION: Taken together, our findings suggest that lupeol could attenuate mancozeb-induced oxidative stress, which in turn could inhibit NF-κB activation and thus provide protection against mancozeb-induced genotoxicity and apoptosis. So, lupeol could be used as a potent anti-oxidant regimen against pesticide induced genotoxicity in agricultural farm workers. [Mh] Termos MeSH primário: Antimutagênicos/farmacologia Fungicidas Industriais/toxicidade Linfócitos/efeitos dos fármacos Maneb/toxicidade Mutagênicos/toxicidade Triterpenos Pentacíclicos/farmacologia Zineb/toxicidade [Mh] Termos MeSH secundário: Ativação Metabólica/efeitos dos fármacos Antioxidantes/metabolismo Apoptose/efeitos dos fármacos Células Cultivadas Aberrações Cromossômicas/efeitos dos fármacos Dano ao DNA Seres Humanos Testes para Micronúcleos NF-kappa B/efeitos dos fármacos [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Antimutagenic Agents); 0 (Antioxidants); 0 (Fungicides, Industrial); 0 (Mutagens); 0 (NF-kappa B); 0 (Pentacyclic Triterpenes); 12427-38-2 (Maneb); O268W13H3O (lupeol); R0HY55EB9E (mancozeb); X1FSB1OZPT (Zineb) [Em] Mês de entrada: 1704 [Cu] Atualização por classe: 170817 [Lr] Data última revisão: 170817 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160529 [St] Status: MEDLINE

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[PMID]: 27208757 [Au] Autor: van Wendel de Joode B; Barbeau B; Bouchard MF; Mora AM; Skytt Å; Córdoba L; Quesada R; Lundh T; Lindh CH; Mergler D [Ad] Endereço: Infants' Environmental Health Program (ISA), Central American Institute for Studies on Toxic Substances (IRET), Universidad Nacional, P.O. Box 86-3000, Heredia, Costa Rica. Electronic address: bernavanwendel@una.cr. [Ti] Título: Manganese concentrations in drinking water from villages near banana plantations with aerial mancozeb spraying in Costa Rica: Results from the Infants' Environmental Health Study (ISA). [So] Source: Environ Pollut;215:247-257, 2016 Aug. [Is] ISSN: 1873-6424 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: Elevated manganese (Mn) in drinking water has been reported worldwide. While, naturally occurring Mn in groundwater is generally the major source, anthropogenic contamination by Mn-containing fungicides such as mancozeb may also occur. The main objective of this study was to examine factors associated with Mn and ethylenethiourea (ETU), a degradation product of mancozeb, in drinking water samples from villages situated near banana plantations with aerial spraying of mancozeb. Drinking water samples (n = 126) were obtained from 124 homes of women participating in the Infants' Environmental Health Study (ISA, for its acronym in Spanish), living nearby large-scale banana plantations. Concentrations of Mn, iron (Fe), arsenic (As), lead (Pb), cadmium (Cd) and ethylenethiourea (ETU), a degradation product of mancozeb, were measured in water samples. Only six percent of samples had detectable ETU concentrations (limit of detection (LOD) = 0.15 µg/L), whereas 94% of the samples had detectable Mn (LOD = 0.05 µg/L). Mn concentrations were higher than 100 and 500 µg/L in 22% and 7% of the samples, respectively. Mn was highest in samples from private and banana farm wells. Distance from a banana plantation was inversely associated with Mn concentrations, with a 61.5% decrease (95% CI: -97.0, -26.0) in Mn concentrations for each km increase in distance. Mn concentrations in water transported with trucks from one village to another were almost 1000 times higher than Mn in water obtained from taps in houses supplied by the same well but not transported, indicating environmental Mn contamination. Elevated Mn in drinking water may be partly explained by aerial spraying of mancozeb; however, naturally occurring Mn in groundwater, and intensive agriculture may also contribute. Drinking water risk assessment for mancozeb should consider Mn as a health hazard. The findings of this study evidence the need for health-based World Health Organization (WHO) guidelines on Mn in drinking water. [Mh] Termos MeSH primário: Poluentes Atmosféricos/análise Água Potável/análise Etilenotioureia/análise Fungicidas Industriais/análise Maneb/análise Manganês/análise Zineb/análise [Mh] Termos MeSH secundário: Adulto Fatores Etários Agricultura Costa Rica Saúde Ambiental Monitoramento Ambiental Feminino Seres Humanos Musa Fatores Socioeconômicos Adulto Jovem [Pt] Tipo de publicação: COMPARATIVE STUDY; JOURNAL ARTICLE [Nm] Nome de substância: 0 (Air Pollutants); 0 (Drinking Water); 0 (Fungicides, Industrial); 12427-38-2 (Maneb); 24FOJ4N18S (Ethylenethiourea); 42Z2K6ZL8P (Manganese); R0HY55EB9E (mancozeb); X1FSB1OZPT (Zineb) [Em] Mês de entrada: 1701 [Cu] Atualização por classe: 170805 [Lr] Data última revisão: 170805 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160522 [St] Status: MEDLINE

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MEDLINE

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[PMID]: 27196962 [Au] Autor: Lafleur B; Sauvé S; Duy SV; Labrecque M [Ad] Endereço: a Institut de recherche en biologie végétale, Université de Montréal , Montréal , QC , Canada. [Ti] Título: Phytoremediation of groundwater contaminated with pesticides using short-rotation willow crops: A case study of an apple orchard. [So] Source: Int J Phytoremediation;18(11):1128-35, 2016 Nov. [Is] ISSN: 1549-7879 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: The occurrence of pesticides in groundwater represents an important health issue, notably for population whose drinking water supply source is located in agricultural areas. However, few solutions have been considered with regard to this issue. We tested the efficacy of a vegetal filtering system made of shrub willows planted at a high density (16,000 plants ha(-1)) to filter or degrade pesticides found in the groundwater flowing out of an apple orchard. Ethylene urea (EU), ethylene thiourea (ETU), tetrahydrophthalimide (THPI), atrazine, and desethylatrazine were monitored in the soil solution in willow and control plots over one growing season. ETU and atrazine concentrations were lower in the willow plots relative to the control plots, whereas desethylatrazine concentration was higher in the willow plots. No significant difference was detected for EU and THPI. Furthermore, pesticide concentrations displayed complex temporal patterns. These results suggest that willow filter systems can filter or degrade pesticides, notably ETU and atrazine, and could be used for phytoremediation purposes. Yet, this potential remains to be quantified with further studies using experimental settings allowing more estimation in time and space. [Mh] Termos MeSH primário: Fungicidas Industriais/metabolismo Água Subterrânea/análise Salix/metabolismo Poluentes do Solo/metabolismo Poluentes Químicos da Água/metabolismo [Mh] Termos MeSH secundário: Biodegradação Ambiental Captana/metabolismo Produtos Agrícolas/crescimento & desenvolvimento Água Potável/análise Malus/crescimento & desenvolvimento Maneb/metabolismo Quebeque Zineb/metabolismo [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Drinking Water); 0 (Fungicides, Industrial); 0 (Soil Pollutants); 0 (Water Pollutants, Chemical); 12427-38-2 (Maneb); EOL5G26Q9F (Captan); R0HY55EB9E (mancozeb); X1FSB1OZPT (Zineb) [Em] Mês de entrada: 1704 [Cu] Atualização por classe: 170406 [Lr] Data última revisão: 170406 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160520 [St] Status: MEDLINE [do] DOI: 10.1080/15226514.2016.1186593

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