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[PMID]: 28364781 [Au] Autor: Feki A; Ben Saad H; Jaballi I; Magne C; Boudawara O; Zeghal KM; Hakim A; Ben Ali Y; Ben Amara I [Ad] Endereço: Higher Institute of Biotechnology of Sfax, 3000 Sfax, Sfax University, Tunisia. [Ti] Título: Methyl thiophanate-induced toxicity in liver and kidney of adult rats: a biochemical, molecular and histopathological approach. [So] Source: Cell Mol Biol (Noisy-le-grand);63(2):20-28, 2017 Feb 28. [Is] ISSN: 1165-158X [Cp] País de publicação: France [La] Idioma: eng [Ab] Resumo: The aim of this study was to elucidate the redox effects of Thiophanate methyl (MT) in the rat liver and kidney. Our results showed, after 3 days of MT injection (700 mg/kg), an increase in malondialdehyde (MDA), hydrogen peroxide and advanced oxidation protein products levels. Glutathione peroxidase and superoxide dismutase activities were also remarkably increased in the liver but decrease in the kidney. Glutathione and vitamin C values were significantly reduced. The changes in biochemical parameters were substantiated by histological and molecular data. A smear without ladder formation on agarose gel was shown, indicating random DNA degradation in the liver and the kidney of MT treated rats. The increase in cyclooxygenase-2 gene expression, marker of inflammation, and an increase in genes expression of glutathione peroxidase and superoxide dismutase in liver and their decrease in the kidney were also occurred after MT exposure. These data confirmed the pro-oxidant and genotoxic effects of this fungicide. [Mh] Termos MeSH primário: Rim/efeitos dos fármacos Fígado/efeitos dos fármacos Tiofanato/toxicidade Testes de Toxicidade Aguda/métodos [Mh] Termos MeSH secundário: Animais Ácido Ascórbico/metabolismo Fragmentação do DNA/efeitos dos fármacos Relação Dose-Resposta a Droga Fungicidas Industriais/administração & dosagem Fungicidas Industriais/toxicidade Expressão Gênica/efeitos dos fármacos Glutationa/metabolismo Glutationa Peroxidase/genética Glutationa Peroxidase/metabolismo Peróxido de Hidrogênio/metabolismo Injeções Intraperitoneais Rim/metabolismo Rim/patologia Dose Letal Mediana Fígado/metabolismo Fígado/patologia Masculino Malondialdeído/metabolismo Oxirredução/efeitos dos fármacos Estresse Oxidativo/efeitos dos fármacos Carbonilação Proteica/efeitos dos fármacos Ratos Wistar Espécies Reativas de Oxigênio/metabolismo Reação em Cadeia da Polimerase Via Transcriptase Reversa Superóxido Dismutase/genética Superóxido Dismutase/metabolismo Tiofanato/administração & dosagem [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Fungicides, Industrial); 0 (Reactive Oxygen Species); 4Y8F71G49Q (Malondialdehyde); 5Q0Y96D5I8 (Thiophanate); BBX060AN9V (Hydrogen Peroxide); EC 1.11.1.9 (Glutathione Peroxidase); EC 1.15.1.1 (Superoxide Dismutase); GAN16C9B8O (Glutathione); PQ6CK8PD0R (Ascorbic Acid) [Em] Mês de entrada: 1704 [Cu] Atualização por classe: 170426 [Lr] Data última revisão: 170426 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170403 [St] Status: MEDLINE [do] DOI: 10.14715/cmb/2017.63.2.4

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[PMID]: 28337501 [Au] Autor: Soliman AS; Helmy RM; Nasr IN; Abbas MS; Mahmoud HA; Jiang W [Ad] Endereço: Natural Resources Department, Institute of African Research and Studies, Cairo University, Giza, 12613, Egypt. [Ti] Título: Behavior of Thiophanate Methyl and Propiconazole in Grape and Mango Fruits Under the Egyptian Field Conditions. [So] Source: Bull Environ Contam Toxicol;98(5):720-725, 2017 May. [Is] ISSN: 1432-0800 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: This research aims at determining residues of thiophanate methyl and propiconazole in grape and mango fruits as an indication for their persistence in this environmental compartment. Fruit extracts were analyzed for thiophanate methyl using High Performance Liquid Chromatography and using Gas Chromatography Electron Capture Detector (GC/ECD), respectively. The results indicated that propiconazole had a less environmental impact since propiconazole had shorter residue half-lives which were 1.24 and 1.19 days in grape and mango fruits, respectively, while thiophanate methyl had half-lives of 2.49 and 2.64 days in mango and grape, respectively. The degradation rates of propiconazole in grape and mango fruits did not change significantly and neither did those of thiophanate methyl. According to the maximum residue level, the pre-harvest intervals of propiconazole were set to be 3 and 7 days for grape and mango fruits, respectively, and the pre-harvest intervals for thiophanate methyl were 15 days for both grape and mango fruits. Propiconazole was generally considered to be less hazardous to humans and will leave the environment less altered because of its faster degradation than that of thiophanate methyl. [Mh] Termos MeSH primário: Frutas/química Mangifera/química Resíduos de Praguicidas/análise Tiofanato/análise Triazóis/análise Vitis/química [Mh] Termos MeSH secundário: Cromatografia Líquida de Alta Pressão Egito Contaminação de Alimentos/análise [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Pesticide Residues); 0 (Triazoles); 142KW8TBSR (propiconazole); 5Q0Y96D5I8 (Thiophanate) [Em] Mês de entrada: 1706 [Cu] Atualização por classe: 170609 [Lr] Data última revisão: 170609 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170325 [St] Status: MEDLINE [do] DOI: 10.1007/s00128-017-2066-x

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[PMID]: 27719947 [Au] Autor: Li JL; Sun DW; Pu H; Jayas DS [Ad] Endereço: School of Food Science and Engineering, South China University of Technology, Guangzhou 510641, China; Academy of Contemporary Food Engineering, South China University of Technology, Guangzhou Higher Education Mega Center, Guangzhou 510006, China. [Ti] Título: Determination of trace thiophanate-methyl and its metabolite carbendazim with teratogenic risk in red bell pepper (Capsicumannuum L.) by surface-enhanced Raman imaging technique. [So] Source: Food Chem;218:543-552, 2017 Mar 01. [Is] ISSN: 0308-8146 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: Surface-enhanced Raman scattering (SERS) imaging coupling with multivariate analysis in spectral region of 200 to 1800cm was developed to quantify and visualize thiophanate-methyl (TM) and its metabolite carbendazim residues in red bell pepper (Capsicum annuum L.). Least squares support vector machines (LS-SVM) and support vector machines (SVM) models based on seven optimized characteristic peaks that showed SERS effects of TM and its metabolite carbendazim residues were employed to establish prediction models. SERS spectra with first derivative (1st) and second derivative (2nd) method were subsequently compared and the optimized model of 1st-LS-SVM acquired showed the best performance (RPD=6.08, R =0.986 and RMSEP=0.473). The results demonstrated that SERS imaging with multivariate analysis had the potential for rapid determination and visualization of the trace TM and its metabolite carbendazim residues in complex food matrices. [Mh] Termos MeSH primário: Benzimidazóis/análise Capsicum/química Carbamatos/análise Análise Espectral Raman/métodos Tiofanato/análise [Mh] Termos MeSH secundário: Benzimidazóis/toxicidade Carbamatos/toxicidade Análise dos Mínimos Quadrados Máquina de Vetores de Suporte Teratogênios/toxicidade [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Benzimidazoles); 0 (Carbamates); 0 (Teratogens); 5Q0Y96D5I8 (Thiophanate); H75J14AA89 (carbendazim) [Em] Mês de entrada: 1701 [Cu] Atualização por classe: 171008 [Lr] Data última revisão: 171008 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161011 [St] Status: MEDLINE

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[PMID]: 27503370 [Au] Autor: Hu MJ; Cox KD; Schnabel G [Ad] Endereço: First and third authors: Department of Agricultural and Environmental Sciences, Clemson University, Clemson, SC; and second author: Department of Plant Pathology and Plant-Microbe Biology, Cornell University, Geneva, NY. [Ti] Título: Resistance to Increasing Chemical Classes of Fungicides by Virtue of "Selection by Association" in Botrytis cinerea. [So] Source: Phytopathology;106(12):1513-1520, 2016 12. [Is] ISSN: 0031-949X [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Previous research has shown that Botrytis cinerea isolates with resistance to multiple chemical classes of fungicides exist in eastern strawberry fields. In this study, the fungicide resistance profiles of 2,130 isolates from flowers of commercial strawberry fields located in multiple states was determined over four consecutive strawberry production seasons. Producers were asked to alternate single-site fungicides that were considered low risk in their specific location based on resistance monitoring results in their fields. This recommendation led to an increase of chemical class diversity used in the spray programs. Results indicated that simultaneous resistance in individual isolates to two, three, four, five, six, and seven classes of fungicides increased over time. The increase in chemical class resistances within isolates was likely due to a process we termed "selection by association", where fungicide resistance traits were often linked to the trait being selected rather than the selectable trait itself. Data analysis also indicated that the odds were highest for isolates resistant to one chemical class (1CCR) to be resistant to thiophanate-methyl; for 2CCR isolates to be resistant to thiophanate-methyl and pyraclostrobin; and for 3CCR isolates to be resistant to thiophanate-methyl, pyraclostrobin, and either cyprodinil or fenhexamid. We hypothesize that the more chemical classes are used in a spray program, the faster isolates will be selected with increasing numbers of chemical class resistances by virtue of selection by association if such isolates preexist in the population. [Mh] Termos MeSH primário: Botrytis/efeitos dos fármacos Farmacorresistência Fúngica/efeitos dos fármacos Fragaria/microbiologia Fungicidas Industriais/farmacologia Doenças das Plantas/prevenção & controle [Mh] Termos MeSH secundário: Botrytis/genética Botrytis/isolamento & purificação Carbamatos/farmacologia Fenótipo Doenças das Plantas/microbiologia Pirazóis/farmacologia Pirimidinas/farmacologia Estrobilurinas Tiofanato/farmacologia [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S. [Nm] Nome de substância: 0 (Carbamates); 0 (Fungicides, Industrial); 0 (Pyrazoles); 0 (Pyrimidines); 0 (Strobilurins); 42P6T6OFWZ (cyprodinil); 5Q0Y96D5I8 (Thiophanate); DJW8M9OX1H (pyrachlostrobin) [Em] Mês de entrada: 1706 [Cu] Atualização por classe: 171116 [Lr] Data última revisão: 171116 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160810 [St] Status: MEDLINE

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[PMID]: 26961913 [Au] Autor: Chayata H; Lassalle Y; Nicol É; Manolikakes S; Souissi Y; Bourcier S; Gosmini C; Bouchonnet S [Ad] Endereço: LCM, CNRS, École Polytechnique, Université Paris-Saclay, 91128 Palaiseau, France. [Ti] Título: Characterization of the ultraviolet-visible photoproducts of thiophanate-methyl using high performance liquid chromatography coupled with high resolution tandem mass spectrometry-Detection in grapes and tomatoes. [So] Source: J Chromatogr A;1441:75-82, 2016 Apr 08. [Is] ISSN: 1873-3778 [Cp] País de publicação: Netherlands [La] Idioma: eng [Ab] Resumo: UV-visible irradiation of thiophanate-methyl (TM) led to the formation of nine photoproducts that were characterized by high performance liquid chromatography coupled with high resolution Fourier transform ion cyclotron resonance mass spectrometry (FTICR-MS). Although carbendazime has been reported in the literature to be the major metabolite and photoproduct of thiophanate-methyl, it was not detected in this study. However, an isomer of carbendazime referred as PP2, which was unambiguously characterized owing to CID experiments, was found in great abundance. Grape berries and cherry tomatoes treated with aqueous solutions of thiophanate-methyl were submitted to irradiation under laboratory conditions. TM and PP2 were detected in both peel and flesh of berries. The ability of TM and PP2 to pass through the fruit skin has been shown to be highly compound and matrix dependent. In vitro bioassays on Vibrio fischeri bacteria showed that the global ecotoxicity of the TM solution increases significantly with the irradiation time. PP2 should likely contribute to this ecotoxicity enhancement since in silico estimations for Daphnia magna provide a LC50 value seven times lower for PP2 than for the parent molecule. [Mh] Termos MeSH primário: Lycopersicon esculentum/química Tiofanato/efeitos da radiação Vitis/química [Mh] Termos MeSH secundário: Aliivibrio fischeri/efeitos dos fármacos Animais Cromatografia Líquida de Alta Pressão/métodos Simulação por Computador Daphnia Frutas/química Luz Fotólise Soluções Espectrometria de Massas em Tandem/métodos Tiofanato/química Tiofanato/toxicidade Fatores de Tempo [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Solutions); 5Q0Y96D5I8 (Thiophanate) [Em] Mês de entrada: 1608 [Cu] Atualização por classe: 160322 [Lr] Data última revisão: 160322 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160311 [St] Status: MEDLINE

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[PMID]: 26697949 [Au] Autor: Zhang D; Park JA; Kim SK; Cho SH; Cho SM; Yi H; Shim JH; Kim JS; Abd El-Aty AM; Shin HC [Ad] Endereço: Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea. [Ti] Título: Determination of residual levels of naloxone, yohimbine, thiophanate, and altrenogest in porcine muscle using QuEChERS with liquid chromatography and triple quadrupole mass spectrometry. [So] Source: J Sep Sci;39(5):835-41, 2016 Mar. [Is] ISSN: 1615-9314 [Cp] País de publicação: Germany [La] Idioma: eng [Ab] Resumo: A quick, easy, cheap, effective, rugged, and safe QuEChERS (method) was used for the simultaneous detection of four veterinary drug residues, namely naloxone, yohimbine, thiophanate, and altrenogest, in porcine muscle, using liquid chromatography with electrospray ionization triple quadrupole tandem mass spectrometry. Because of the unavailability of a suitable internal standard, matrix-matched calibrations were used for quantification, with determination coefficients ≥ 0.9542. The accuracy (expressed as recovery %) ranged from 60.53 to 83.25%, and the intra- and interday precisions (expressed as relative standard deviations) were <12%. The limits of quantification were 5, 0.5, 2, and 5 ng/g for naloxone, yohimbine, thiophanate, and altrenogest, respectively. Samples purchased from local markets in Seoul, Republic of Korea, revealed no traces of the target analytes. The developed method described herein is sensitive and reliable and can be applied to quantify the tested veterinary drugs in animal tissues. [Mh] Termos MeSH primário: Resíduos de Drogas/isolamento & purificação Músculos/química Naloxona/isolamento & purificação Extração em Fase Sólida/métodos Tiofanato/isolamento & purificação Acetato de Trembolona/análogos & derivados Drogas Veterinárias/isolamento & purificação Ioimbina/isolamento & purificação [Mh] Termos MeSH secundário: Animais Cromatografia Líquida de Alta Pressão Resíduos de Drogas/análise Contaminação de Alimentos/análise Limite de Detecção Carne/análise Naloxona/análise Suínos Espectrometria de Massas em Tandem Tiofanato/análise Acetato de Trembolona/análise Acetato de Trembolona/isolamento & purificação Drogas Veterinárias/análise Ioimbina/análise [Pt] Tipo de publicação: EVALUATION STUDIES; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Veterinary Drugs); 2U0X0JA2NB (altrenogest); 2Y49VWD90Q (Yohimbine); 36B82AMQ7N (Naloxone); 5Q0Y96D5I8 (Thiophanate); RUD5Y4SV0S (Trenbolone Acetate) [Em] Mês de entrada: 1612 [Cu] Atualização por classe: 161230 [Lr] Data última revisão: 161230 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 151225 [St] Status: MEDLINE [do] DOI: 10.1002/jssc.201501206

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[PMID]: 25418926 [Au] Autor: Bellón-Gómez D; Vela-Corcía D; Pérez-García A; Torés JA [Ad] Endereço: Instituto de Hortofruticultura Subtropical y Mediterránea 'La Mayora', Universidad de Málaga - Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC), Estación Experimental 'La Mayora', Algarrobo-Costa, Málaga, Spain. [Ti] Título: Sensitivity of Podosphaera xanthii populations to anti-powdery-mildew fungicides in Spain. [So] Source: Pest Manag Sci;71(10):1407-13, 2015 Oct. [Is] ISSN: 1526-4998 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: BACKGROUND: Cucurbit powdery mildew caused by Podosphaera xanthii limits crop production in Spain, where disease control is largely dependent on fungicides. In previous studies, high levels of resistance to QoI and DMI fungicides were documented in south-central Spain. The aim of this study was to investigate the sensitivity of P. xanthii populations to other fungicides and to provide tools for improved disease management. RESULTS: Using a leaf-disc assay, sensitivity to thiophanate-methyl, bupirimate and quinoxyfen of 50 isolates of P. xanthii was analysed to determine discriminatory concentrations between sensitive and resistant isolates. With the exception of thiophanate-methyl, no clearly different groups of isolates could be identified, and as a result, discriminatory concentrations were established on the basis of the maximum fungicide field application rate. Subsequently, a survey of P. xanthii resistance to these fungicides was carried out by testing a collection of 237 isolates obtained during the 2002-2011 cucurbit growing seasons. This analysis revealed very high levels of resistance to thiophanate-methyl (95%). By contrast, no resistance to bupirimate and quinoxyfen was found. CONCLUSION: Results suggest that thiophanate-methyl has become completely ineffective for controlling cucurbit powdery mildew in Spain. By contrast, bupirimate and quinoxyfen remain as very effective tools for cucurbit powdery mildew management. © 2014 Society of Chemical Industry. [Mh] Termos MeSH primário: Ascomicetos/efeitos dos fármacos Cucurbita/microbiologia Fungicidas Industriais/farmacologia Doenças das Plantas/microbiologia [Mh] Termos MeSH secundário: Ascomicetos/fisiologia Farmacorresistência Fúngica Doenças das Plantas/prevenção & controle Espanha Tiofanato/farmacologia [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Fungicides, Industrial); 5Q0Y96D5I8 (Thiophanate) [Em] Mês de entrada: 1602 [Cu] Atualização por classe: 150903 [Lr] Data última revisão: 150903 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 141125 [St] Status: MEDLINE [do] DOI: 10.1002/ps.3943

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[PMID]: 25179310 [Au] Autor: Ben Amara I; Ben Saad H; Cherif B; Elwej A; Lassoued S; Kallel C; Zeghal N [Ad] Endereço: Animal Physiology Laboratory, Faculty of Sciences, University of Sfax , Tunisia . [Ti] Título: Methyl-thiophanate increases reactive oxygen species production and induces genotoxicity in rat peripheral blood. [So] Source: Toxicol Mech Methods;24(9):679-87, 2014 Dec. [Is] ISSN: 1537-6524 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: Methylthiophanate is one of the widely used fungicides to control important fungal diseases of crops. The aim of this study was to elucidate the short-term hematoxicity and genotoxicity effects of methylthiophanate administered by intraperitoneal way at three doses (300, 500 and 700 mg/kg of body weight) after 24, 48 and 72 h. Our results showed, 24 h after methylthiophanate injection, a hematological perturbation such as red blood cells (p < 0.05, p < 0.05 and p < 0.01) and hemoglobin content (p < 0.05), respectively, and a noticeable genotoxic effect in WBC evidenced by a significant increase in the frequency of the micronuclei and a decrease in cell viability. An increase in erythrocyte osmotic fragility was also noted after 24 and 48 h of methylthiophanate treatment at graded doses. A significant increase in hydrogen peroxide, advanced oxidation of protein products and malondialdehyde levels, in erythrocytes of methylthiophanate-treated rats with 300, 500 and 700 mg/kg of body weight, was also observed after 24 h of treatment (p < 0.05, p < 0.01 and p < 0.001, respectively), suggesting the implication of oxidative stress in its toxicity. Antioxidants activities of superoxide dismutase and glutathione peroxidase in erythrocytes significantly increased (p < 0.001) 24 h after the highest dose injected. While all these parameters were improved after 72 h of methylthiophanate injection (300, 500 and 700 mg/kg body weight). In conclusion, these data showed that the exposure of adult rats to methylthiophanate resulted in oxidative stress leading to hematotoxicity and the impairment of defence system, confirming the pro-oxidant and genotoxic effects of this fungicide. [Mh] Termos MeSH primário: Espécies Reativas de Oxigênio/metabolismo Tiofanato/farmacologia [Mh] Termos MeSH secundário: Animais Dose Letal Mediana Masculino Ratos Ratos Wistar Tiofanato/química [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Reactive Oxygen Species); 5Q0Y96D5I8 (Thiophanate) [Em] Mês de entrada: 1507 [Cu] Atualização por classe: 141124 [Lr] Data última revisão: 141124 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 140903 [St] Status: MEDLINE [do] DOI: 10.3109/15376516.2014.961217

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[PMID]: 23775824 [Au] Autor: Fan J; Luo Y; Michailides TJ; Guo L [Ad] Endereço: Department of Plant Pathology, China Agricultural University, Beijing, 100193, P.R. China. [Ti] Título: Simultaneous quantification of alleles E198A and H6Y in the ß-tubulin gene conferring benzimidazole resistance in Monilinia fructicola using a duplex real-time (TaqMan) PCR. [So] Source: Pest Manag Sci;70(2):245-51, 2014 Feb. [Is] ISSN: 1526-4998 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: BACKGROUND: The benzimidazole fungicide thiophanate-methyl is commonly used for the control of brown rot of stone fruits. Low and high levels of resistance to this fungicide have been found in field isolates of the causal pathogen Monilinia fructicola. RESULTS: The minor groove binding (MGB) TaqMan probes specific for alleles E198A and H6Y conferring the high and low levels of resistance in the ß-tubulin gene of M. fructicola were designed. A duplex real-time PCR assay based on these probes was developed for simultaneous quantification of both mutations in a pathogen population. The specificity tests for the primers and probes were conducted using different fungal species of stone and pome fruit pathogens. Similar results were obtained between the duplex real-time (TaqMan) PCR assay and the conventional method to quantify the frequencies of alleles E198A and H6Y of eight samples from different peach orchards. CONCLUSION: The MGB TaqMan probe based duplex real-time PCR provides a useful tool for simultaneous quantification of both alleles, E198A and H6Y, conferring high and low resistance, and has a potential in monitoring the benzimidazole-resistance in M. fructicola populations in stone and pome fruit orchards. [Mh] Termos MeSH primário: Alelos Ascomicetos/genética Benzimidazóis/farmacologia Farmacorresistência Fúngica/genética Reação em Cadeia da Polimerase em Tempo Real/métodos Taq Polimerase/metabolismo Tubulina (Proteína)/genética [Mh] Termos MeSH secundário: Ascomicetos/efeitos dos fármacos Primers do DNA/genética Fungicidas Industriais/farmacologia Frequência do Gene Tiofanato/farmacologia [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Benzimidazoles); 0 (DNA Primers); 0 (Fungicides, Industrial); 0 (Tubulin); 5Q0Y96D5I8 (Thiophanate); E24GX49LD8 (benzimidazole); EC 2.7.7.- (Taq Polymerase) [Em] Mês de entrada: 1409 [Cu] Atualização por classe: 140114 [Lr] Data última revisão: 140114 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 130619 [St] Status: MEDLINE [do] DOI: 10.1002/ps.3549

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[PMID]: 23722654 [Au] Autor: Ferreira JA; Santos LF; Souza NR; Navickiene S; de Oliveira FA; Talamini V [Ad] Endereço: Departamento de Química, Universidade Federal de Sergipe, Av. Marechal Rondon s/n, São Cristóvão, SE 49100-000, Brazil. [Ti] Título: MSPD sample preparation approach for reversed-phase liquid chromatographic analysis of pesticide residues in stem of coconut palm. [So] Source: Bull Environ Contam Toxicol;91(2):160-4, 2013 Aug. [Is] ISSN: 1432-0800 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: A method was developed using matrix solid-phase dispersion, together with liquid chromatography with ultraviolet diode array detector for determination of carbofuran, difenoconazole, ß-cyfluthrin, spirodiclofen and thiophanate-methyl in stem of coconut palm. The best results were obtained using 2.0 g of stem, 1.6 g of Florisil as sorbent and cyclohexane:acetone mixture (4:1). The method was validated using stem samples spiked with pesticides at four concentration levels (0.05-2.0 µg/g). Average recoveries ranged from 70 % to 114.3 %, with relative standard deviations between 1.2 % and 19.2 %. Detection and quantification limits were in the ranges 0.02-0.03 and 0.05-0.1 µg/g, respectively. [Mh] Termos MeSH primário: Cromatografia Líquida de Alta Pressão/métodos Cocos/química Resíduos de Praguicidas/análise Caules de Planta/química Extração em Fase Sólida/métodos [Mh] Termos MeSH secundário: 4-Butirolactona/análogos & derivados 4-Butirolactona/análise Acetona/química Carbofurano/análise Cicloexanos/química Dioxolanos/análise Silicatos de Magnésio/química Nitrilos/análise Piretrinas/análise Reprodutibilidade dos Testes Compostos de Espiro/análise Tiofanato/análise Triazóis/análise [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Cyclohexanes); 0 (Dioxolanes); 0 (Magnesium Silicates); 0 (Nitriles); 0 (Pesticide Residues); 0 (Pyrethrins); 0 (Spiro Compounds); 0 (Triazoles); 1343-88-0 (Florisil); 1364PS73AF (Acetone); 3X7G31F5MX (spirodiclofen); 48K5MKG32S (Cyclohexane); 5Q0Y96D5I8 (Thiophanate); D9612XCH4P (difenoconazole); OL659KIY4X (4-Butyrolactone); SCM2QLZ6S0 (cyfluthrin); SKF77S6Y67 (Carbofuran) [Em] Mês de entrada: 1402 [Cu] Atualização por classe: 171116 [Lr] Data última revisão: 171116 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 130601 [St] Status: MEDLINE [do] DOI: 10.1007/s00128-013-1018-3

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