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[PMID]:29232952
[Au] Autor:Du H; Song Z; Xu Y
[Ad] Endereço:The Key Laboratory of Industrial Biotechnology of the Ministry of Education, State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Biotechnology, Jiangnan University , 1800 Lihu Avenue, Wuxi, Jiangsu 214122, China.
[Ti] Título:Ethyl Carbamate Formation Regulated by Lactic Acid Bacteria and Nonconventional Yeasts in Solid-State Fermentation of Chinese Moutai-Flavor Liquor.
[So] Source:J Agric Food Chem;66(1):387-392, 2018 Jan 10.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:This study aimed to identify specific microorganisms related to the formation of precursors of EC (ethyl carbamate) in the solid-state fermentation of Chinese Moutai-flavor liquor. The EC content was significantly correlated with the urea content during the fermentation process (R = 0.772, P < 0.01). Differences in urea production and degradation were found at both species and functional gene levels by metatranscriptomic sequencing and culture-dependent analysis. Lactobacillus spp. could competitively degrade arginine through the arginine deiminase pathway with yeasts, and most Lactobacillus species were capable of degrading urea. Some dominant nonconventional yeasts, such as Pichia, Schizosaccharomyces, and Zygosaccharomyces species, were shown to produce low amounts of urea relative to Saccharomyces cerevisiae. Moreover, unusual urea degradation pathways (urea carboxylase, allophanate hydrolase, and ATP-independent urease) were identified. Our results indicate that EC precursor levels in the solid-state fermentation can be controlled using lactic acid bacteria and nonconventional yeasts.
[Mh] Termos MeSH primário: Lactobacillus/metabolismo
Uretana/metabolismo
Vinho/análise
Leveduras/metabolismo
[Mh] Termos MeSH secundário: Etanol/análise
Etanol/metabolismo
Fermentação
Aromatizantes/análise
Aromatizantes/metabolismo
Seres Humanos
Paladar
Uretana/análise
Vinho/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Flavoring Agents); 3IN71E75Z5 (Urethane); 3K9958V90M (Ethanol)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180205
[Lr] Data última revisão:
180205
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171214
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b05034


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[PMID]:28946124
[Au] Autor:Lee GH; Bang DY; Lim JH; Yoon SM; Yea MJ; Chi YM
[Ad] Endereço:Division of Biotechnology, College of Life Sciences, Korea University, Seoul 02841, Republic of Korea.
[Ti] Título:Simultaneous determination of ethyl carbamate and urea in Korean rice wine by ultra-performance liquid chromatography coupled with mass spectrometric detection.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;1065-1066:44-49, 2017 Oct 15.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:In this study, a rapid method for simultaneous detection of ethyl carbamate (EC) and urea in Korean rice wine was developed. To achieve quantitative analysis of EC and urea, the conditions for Ultra-performance liquid chromatography (UPLC) separation and atmospheric-pressure chemical ionization tandem mass spectrometry (APCI-MS/MS) detection were first optimized. Under the established conditions, the detection limit, relative standard deviation and linear range were 2.83µg/L, 3.75-5.96%, and 0.01-10.0mg/L, respectively, for urea; the corresponding values were 0.17µg/L, 1.06-4.01%, and 1.0-50.0µg/L, respectively, for EC. The correlation between the contents of EC and its precursor urea was determined under specific pH (3.5 and 4.5) and temperature (4, 25, and 50°C) conditions using the developed method. As a result, EC content was increased with greater temperature and lower pH. In Korean rice wine, urea was detected 0.19-1.37mg/L and EC was detected 2.0-7.7µg/L. The method developed in this study, which has the advantages of simplified sample preparation, low detection limits, and good selectivity, was successfully applied for the rapid analysis of EC and urea.
[Mh] Termos MeSH primário: Cromatografia Líquida de Alta Pressão/métodos
Cromatografia Gasosa-Espectrometria de Massas/métodos
Ureia/análise
Uretana/análise
Vinho/análise
[Mh] Termos MeSH secundário: Limite de Detecção
Modelos Lineares
Oryza
Reprodutibilidade dos Testes
República da Coreia
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
3IN71E75Z5 (Urethane); 8W8T17847W (Urea)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171030
[Lr] Data última revisão:
171030
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170926
[St] Status:MEDLINE


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[PMID]:28818685
[Au] Autor:Liu H; Cui B; Xu Y; Hu C; Liu Y; Qu G; Li D; Wu Y; Zhang D; Quan S; Shi J
[Ad] Endereço:Joint International Research Laboratory of Metabolic & Developmental Sciences, SJTU-University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Rd., Shanghai 200240, China.
[Ti] Título:Ethyl carbamate induces cell death through its effects on multiple metabolic pathways.
[So] Source:Chem Biol Interact;277:21-32, 2017 Nov 01.
[Is] ISSN:1872-7786
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:Ethyl carbamate (EC), a multisite carcinogenic chemical causing tumors in various animal species, is probably carcinogenic to humans. However, information about the possible carcinogenic and toxicological effects of EC in humans is quite limited. Because EC is found in many dietary foods (such as fermented foods) and tobacco and its products, and exposure of humans to EC often occurs inevitably, its toxicological effects in humans need to be studied. This study was conducted to understand the metabolomic and transcriptomic changes in human hepatocellular carcinoma cells (HepG2) exposed to 100 mM EC for short term (4 h) and long term (12 h) period, respectively. The results revealed multiple influences of EC on the metabolome and transcriptome of HepG2 cells, which was exposure time-dependent and well correlated with the kinetic changes of cell viability and mortality. EC treatment affected multiple metabolic pathways, inducing oxidative stress, reducing detoxification capacity, depleting energy, decreasing reducing power, disrupting membrane integrity, and damaging DNA and protein. These metabolomic and transcriptomic biomarkers of EC on human cell metabolism identified in this study would facilitate further studies on the risk assessment and the mitigation of dietary EC.
[Mh] Termos MeSH primário: Antineoplásicos/farmacologia
Carcinoma Hepatocelular/tratamento farmacológico
Morte Celular/efeitos dos fármacos
Neoplasias Hepáticas/tratamento farmacológico
Redes e Vias Metabólicas/efeitos dos fármacos
Uretana/farmacologia
[Mh] Termos MeSH secundário: Carcinoma Hepatocelular/genética
Carcinoma Hepatocelular/metabolismo
Células Hep G2
Seres Humanos
Neoplasias Hepáticas/genética
Neoplasias Hepáticas/metabolismo
Estresse Oxidativo/efeitos dos fármacos
Transcriptoma/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents); 3IN71E75Z5 (Urethane)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171108
[Lr] Data última revisão:
171108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170819
[St] Status:MEDLINE


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[PMID]:28783003
[Au] Autor:Choi B; Ryu D; Kim CI; Lee JY; Choi A; Koh E
[Ad] Endereço:a Major of Food & Nutrition, Division of Applied Food System , Seoul Women's University , Seoul , Korea.
[Ti] Título:Probabilistic dietary exposure to ethyl carbamate from fermented foods and alcoholic beverages in the Korean population.
[So] Source:Food Addit Contam Part A Chem Anal Control Expo Risk Assess;34(11):1885-1892, 2017 Nov.
[Is] ISSN:1944-0057
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The occurrence of ethyl carbamate was investigated in fermented foods and alcoholic beverages of the Korean total diet study. The concentrations of ethyl carbamate ranged from not detected to 166.5 µg kg . Dietary exposure to ethyl carbamate was estimated by the probabilistic method. Estimated intakes of ethyl carbamate from foods and alcoholic beverages were 4.12 ng kg body weight (bw) per day for average consumers and 12.37 ng kg bw/day for 95th percentile high consumers. The major foods contributing to ethyl carbamate exposure were soy sauce (63%), followed by maesilju (plum liqueur, 30%), whisky (5%), and bokbunjaju (black raspberry wine, 2%). On the basis of the benchmark dose lower confidence limit 10% (BMDL ) of 0.3 mg kg bw/day, margins of exposure were 128,000 for mean exposure and 40,000 for 95th percentile exposure. This indicates that the exposure of the Korean general population for ethyl carbamate is of low concern. However, careful vigilance should be continued for high consumers of fermented foods and alcoholic beverages.
[Mh] Termos MeSH primário: Bebidas Alcoólicas/análise
Carcinógenos/administração & dosagem
Carcinógenos/análise
Dieta
Fermentação
Contaminação de Alimentos/análise
Uretana/administração & dosagem
Uretana/análise
[Mh] Termos MeSH secundário: Adolescente
Adulto
Idoso
Criança
Pré-Escolar
Feminino
Seres Humanos
Lactente
Masculino
Meia-Idade
República da Coreia
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Carcinogens); 3IN71E75Z5 (Urethane)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170808
[St] Status:MEDLINE
[do] DOI:10.1080/19440049.2017.1364433


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[PMID]:28721728
[Au] Autor:Ma L; Huang S; Du L; Tang P; Xiao D
[Ad] Endereço:Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin Industrial Microbiology Key Laboratory, College of Biotechnology, Tianjin University of Science and Technology , Tianjin 300457, People's Republic of China.
[Ti] Título:Reduced Production of Higher Alcohols by Saccharomyces cerevisiae in Red Wine Fermentation by Simultaneously Overexpressing BAT1 and Deleting BAT2.
[So] Source:J Agric Food Chem;65(32):6936-6942, 2017 Aug 16.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In red wine, the contents of higher alcohols and ethyl carbamate (EC) are two significant health concerns. To reduce the production of higher alcohols by wine yeast YZ22 with low production of EC, one BAT2 was replaced by a BAT1 expression cassette first and then another BAT2 was deleted to obtain the mutant SYBB3. Real-time quantitative PCR showed that the relative expression level of BAT1 in SYBB3 improved 28 times compared with that in YZ22. The yields of isobutanol and 3-methyl-1-butanol produced by mutant SYBB3 reduced by 39.41% and 37.18% compared to those by the original strain YZ22, and the total production of higher alcohols decreased from 463.82 mg/L to 292.83 mg/L in must fermentation of Cabernet Sauvignon. Meanwhile, there were no obvious differences on fermentation characteristics of the mutant and parental strain. This research has suggested an effective strategy for decreasing production of higher alcohols in Saccharomyces cerevisiae.
[Mh] Termos MeSH primário: Álcoois/metabolismo
Proteínas Mitocondriais/genética
Proteínas de Saccharomyces cerevisiae/genética
Saccharomyces cerevisiae/genética
Saccharomyces cerevisiae/metabolismo
Transaminases/genética
Vinho/análise
[Mh] Termos MeSH secundário: Álcoois/análise
Fermentação
Frutas/metabolismo
Frutas/microbiologia
Proteínas Mitocondriais/metabolismo
Proteínas de Saccharomyces cerevisiae/metabolismo
Transaminases/metabolismo
Uretana/análise
Uretana/metabolismo
Vitis/metabolismo
Vitis/microbiologia
Vinho/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alcohols); 0 (Mitochondrial Proteins); 0 (Saccharomyces cerevisiae Proteins); 3IN71E75Z5 (Urethane); EC 2.6.1.- (Transaminases); EC 2.6.1.42 (BAT1 protein, S cerevisiae); EC 2.6.1.42 (BAT2 protein, S cerevisiae)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170825
[Lr] Data última revisão:
170825
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170720
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b01974


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[PMID]:28530563
[Au] Autor:Zhou W; Fang R; Chen Q
[Ad] Endereço:Department of Food Science and Nutrition, Zhejiang University, Hangzhou 310058, China.
[Ti] Título:Effect of gallic and protocatechuic acids on the metabolism of ethyl carbamate in Chinese yellow rice wine brewing.
[So] Source:Food Chem;233:174-181, 2017 Oct 15.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:It was studied that gallic and protocatechuic acids played important roles in ethyl carbamate (EC) forming. Gallic and protocatechuic acids can reduce the arginine consumption through inhibiting the arginine deiminase enzyme. Therefore, they are generally added to regulate EC catabolism in the course of yellow rice wine leavening at the third day. In this work, gallic and protocatechuic acids made little influence on the growth of Saccharomyces cerevisiae. Besides, the addition of 200mg/L gallic or protocatechuic acid could prevent the transformation from urea/citrulline to EC. Gallic acid showed better inhibiting effect that the content of EC could be reduced by 91.9% at most. Furthermore, the production of amino acids and volatile flavor compounds are not markedly affected by phenolic compounds. The discoveries reveal that EC can be reduced by supplying gallic acid or protocatechuic acid while yellow rice wine leavening.
[Mh] Termos MeSH primário: Oryza
Vinho
[Mh] Termos MeSH secundário: Fermentação
Alho
Saccharomyces cerevisiae
Ureia
Uretana
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
3IN71E75Z5 (Urethane); 8W8T17847W (Urea)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170811
[Lr] Data última revisão:
170811
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170523
[St] Status:MEDLINE


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[PMID]:28372175
[Au] Autor:Vázquez L; Prados IM; Reglero G; Torres CF
[Ad] Endereço:Departamento de Producción y Caracterización de Nuevos Alimentos, Instituto de Investigación en Ciencias de la Alimentación (CSIC-UAM), C/ Nicolás Cabrera 9, Universidad Autónoma de Madrid, 28049 Madrid, Spain. Electronic address: luis.vazquez@uam.es.
[Ti] Título:Identification and quantification of ethyl carbamate occurring in urea complexation processes commonly utilized for polyunsaturated fatty acid concentration.
[So] Source:Food Chem;229:28-34, 2017 Aug 15.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The concentration of polyunsaturated fatty acids by formation of urea adducts from three different sources was studied to elucidate the formation of ethyl carbamates in the course of these procedures. Two different methodologies were performed: with ethanol at high temperature and with hexane/ethanol mixtures at room temperature. It was proved that the amount of urethanes generated at high temperature was higher than at room temperature. Besides, subsequent washing steps of the PUFA fraction with water were efficient to remove the urethanes from the final products. The methodology at room temperature with 0.4mL ethanol and 3g urea provided good relationship between concentration and yield of the main bioactive PUFA, with the lowest formation of ethyl carbamates in the process.
[Mh] Termos MeSH primário: Ácidos Graxos Insaturados/química
Ureia/química
Uretana/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fatty Acids, Unsaturated); 3IN71E75Z5 (Urethane); 8W8T17847W (Urea)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170503
[Lr] Data última revisão:
170503
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170405
[St] Status:MEDLINE


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[PMID]:28240047
[Au] Autor:Hamzawy MA; Abo-Youssef AM; Salem HF; Mohammed SA
[Ad] Endereço:a Pharmacology and Toxicology Department, College of Pharmacy, Misr University for Science and Technology , 6th October City , Egypt.
[Ti] Título:Antitumor activity of intratracheal inhalation of temozolomide (TMZ) loaded into gold nanoparticles and/or liposomes against urethane-induced lung cancer in BALB/c mice.
[So] Source:Drug Deliv;24(1):599-607, 2017 Nov.
[Is] ISSN:1521-0464
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The current study aimed to develop gold nanoparticles (GNPs) and liposome-embedded gold nanoparticles (LGNPs) as drug carriers for temozolomide (TMZ) and investigate the possible therapeutic effects of intratracheal inhalation of nanoformulation of TMZ-loaded gold nanoparticles (TGNPs) and liposome-embedded TGNPs (LTGNPs) against urethane-induced lung cancer in BALB/c mice. Physicochemical characters and zeta potential studies for gold nanoparticles (GNPs) and liposome-embedded gold nanoparticles (LGNPs) were performed. The current study was conducted by inducing lung cancer chemically via repeated exposure to urethane in BALB/C mice. GNPs and LGNPs were exhibited in uniform spherical shape with adequate dispersion stability. GNPs and LGNPs showed no significant changes in comparison to control group with high safety profile, while TGNPs and LTGNPs succeed to improve all biochemical data and histological patterns. GNPs and LGNPs are promising drug carriers and succeeded in the delivery of small and efficient dose of temozolomide in treatment lung cancer. Antitumor activity was pronounced in animal-treated LTGNPs, these effects may be due to synergistic effects resulted from combination of temozolomide and gold nanoparticles and liposomes that may improve the drug distribution and penetration.
[Mh] Termos MeSH primário: Antineoplásicos Alquilantes/administração & dosagem
Dacarbazina/análogos & derivados
Ouro/química
Lipídeos/química
Neoplasias Pulmonares/tratamento farmacológico
Nanopartículas Metálicas
Uretana
[Mh] Termos MeSH secundário: Administração por Inalação
Animais
Antineoplásicos Alquilantes/química
Biomarcadores Tumorais/sangue
Dacarbazina/administração & dosagem
Dacarbazina/química
Composição de Medicamentos
Estabilidade de Medicamentos
Lipossomos
Neoplasias Pulmonares/sangue
Neoplasias Pulmonares/induzido quimicamente
Neoplasias Pulmonares/patologia
Masculino
Camundongos Endogâmicos BALB C
Nanomedicina/métodos
Tamanho da Partícula
Carga Tumoral/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents, Alkylating); 0 (Biomarkers, Tumor); 0 (Lipids); 0 (Liposomes); 3IN71E75Z5 (Urethane); 7440-57-5 (Gold); 7GR28W0FJI (Dacarbazine); YF1K15M17Y (temozolomide)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170321
[Lr] Data última revisão:
170321
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170228
[St] Status:MEDLINE
[do] DOI:10.1080/10717544.2016.1247924


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[PMID]:28237801
[Au] Autor:Zakowski W; Braszka L; Zawistowski P; Orzel-Gryglewska J; Jurkowlaniec E
[Ad] Endereço:Department of Animal and Human Physiology, Faculty of Biology, University of Gdansk, Wita Stwosza 59, 80-308 Gdansk, Poland. Electronic address: witold.zakowski@biol.ug.edu.pl.
[Ti] Título:Inactivation of the medial mammillary nucleus attenuates theta rhythm activity in the hippocampus in urethane-anesthetized rats.
[So] Source:Neurosci Lett;645:19-24, 2017 04 03.
[Is] ISSN:1872-7972
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:Although the importance of the mammillary body for memory and learning processes is well known, its exact role has remained vague. The fact, that many neurons in one nucleus of the mammillary body in rats, i.e. the medial mammillary nucleus (MM), fires according with hippocampal theta rhythm, makes this structure crucial for a theta rhythm signaling in so-called extended hippocampal system. These neurons are driven by descending projections from the hippocampal formation, but it is still unknown whether the mammillary body only conveys theta rhythm or may also modulate it. In the present study, we investigated the effect of pharmacological inactivation (local infusion of 0.5µl of 20% procaine hydrochloride solution) of the MM on hippocampal theta rhythm in urethane-anesthetized rats. We found that intra-MM procaine microinjections suppress sensory-elicited theta rhythm in the hippocampus by reduction of its amplitude, but not the frequency. Procaine infusion decreased the EEG signal power of low theta frequency bands, i.e. 3-5Hz, down to 9.2% in 3-4Hz band in comparison to pre-injection conditions. After water infusion (control group) no changes of hippocampal EEG signal power were observed. Our findings showed for the first time that inactivation of the MM leads to a disruption of hippocampal theta rhythm in the rat, which may suggest that the mammillary body can regulate theta rhythm signaling in the extended hippocampal system.
[Mh] Termos MeSH primário: Anestésicos Intravenosos
Hipotálamo Posterior/fisiopatologia
Ritmo Teta
Uretana
[Mh] Termos MeSH secundário: Anestésicos Locais/farmacologia
Animais
Masculino
Corpos Mamilares/fisiopatologia
Microinjeções
Procaína/farmacologia
Ratos Wistar
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anesthetics, Intravenous); 0 (Anesthetics, Local); 3IN71E75Z5 (Urethane); 4Z8Y51M438 (Procaine)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171101
[Lr] Data última revisão:
171101
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170227
[St] Status:MEDLINE


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[PMID]:28219725
[Au] Autor:Tsytsarev V; Akkentli F; Pumbo E; Tang Q; Chen Y; Erzurumlu RS; Papkovsky DB
[Ad] Endereço:Department of Anatomy and Neurobiology, University of Maryland School of Medicine, 20 Penn Street, HSF-2, 21201 MD, Baltimore, USA. Electronic address: tsytsarev@som.umaryland.edu.
[Ti] Título:Planar implantable sensor for in vivo measurement of cellular oxygen metabolism in brain tissue.
[So] Source:J Neurosci Methods;281:1-6, 2017 Apr 01.
[Is] ISSN:1872-678X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Brain imaging methods are continually improving. Imaging of the cerebral cortex is widely used in both animal experiments and charting human brain function in health and disease. Among the animal models, the rodent cerebral cortex has been widely used because of patterned neural representation of the whiskers on the snout and relative ease of activating cortical tissue with whisker stimulation. NEW METHOD: We tested a new planar solid-state oxygen sensor comprising a polymeric film with a phosphorescent oxygen-sensitive coating on the working side, to monitor dynamics of oxygen metabolism in the cerebral cortex following sensory stimulation. RESULTS: Sensory stimulation led to changes in oxygenation and deoxygenation processes of activated areas in the barrel cortex. We demonstrate the possibility of dynamic mapping of relative changes in oxygenation in live mouse brain tissue with such a sensor. COMPARISON WITH EXISTING METHOD: Oxygenation-based functional magnetic resonance imaging (fMRI) is very effective method for functional brain mapping but have high costs and limited spatial resolution. Optical imaging of intrinsic signal (IOS) does not provide the required sensitivity, and voltage-sensitive dye optical imaging (VSDi) has limited applicability due to significant toxicity of the voltage-sensitive dye. Our planar solid-state oxygen sensor imaging approach circumvents these limitations, providing a simple optical contrast agent with low toxicity and rapid application. CONCLUSIONS: The planar solid-state oxygen sensor described here can be used as a tool in visualization and real-time analysis of sensory-evoked neural activity in vivo. Further, this approach allows visualization of local neural activity with high temporal and spatial resolution.
[Mh] Termos MeSH primário: Imagem Óptica/instrumentação
Oxigênio/análise
Córtex Somatossensorial/química
[Mh] Termos MeSH secundário: Anestésicos Intravenosos/farmacologia
Anestésicos Intravenosos/toxicidade
Animais
Potenciais Somatossensoriais Evocados/fisiologia
Feminino
Neuroimagem Funcional/instrumentação
Neuroimagem Funcional/métodos
Masculino
Camundongos
Imagem Óptica/métodos
Estimulação Física
Córtex Somatossensorial/fisiologia
Percepção do Tato/fisiologia
Uretana/farmacologia
Uretana/toxicidade
Vibrissas/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anesthetics, Intravenous); 3IN71E75Z5 (Urethane); S88TT14065 (Oxygen)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171113
[Lr] Data última revisão:
171113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170222
[St] Status:MEDLINE



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