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[PMID]:28749499
[Au] Autor:Horbury MD; Quan WD; Flourat AL; Allais F; Stavros VG
[Ad] Endereço:Department of Chemistry, University of Warwick, Gibbet Hill, Coventry, CV4 7AL, UK. v.stavros@warwick.ac.uk.
[Ti] Título:Elucidating nuclear motions in a plant sunscreen during photoisomerization through solvent viscosity effects.
[So] Source:Phys Chem Chem Phys;19(31):21127-21131, 2017 Aug 09.
[Is] ISSN:1463-9084
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:We explore the effects of solvent viscosity on the trans-cis photoisomerization of sinapoyl malate, which is utilized as a sunscreen molecule in plants. Our results demonstrate that viscosity has a significant effect on the timescale for isomerization, providing insight into the nuclear motions involved. The ramifications of these findings are discussed with reference to sinapoyl malate's in vivo photoprotection properties.
[Mh] Termos MeSH primário: Malatos/química
Fenilpropionatos/química
Plantas/química
Solventes/química
Protetores Solares/química
[Mh] Termos MeSH secundário: Isomerismo
Plantas/metabolismo
Espectrofotometria
Raios Ultravioleta
Viscosidade/efeitos da radiação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (2-O-sinapoylmalate); 0 (Malates); 0 (Phenylpropionates); 0 (Solvents); 0 (Sunscreening Agents)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170728
[St] Status:MEDLINE
[do] DOI:10.1039/c7cp04070a


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[PMID]:29199224
[Au] Autor:Suzuki R; Kan S; Sugita Y; Shirataki Y
[Ad] Endereço:Department of Pharmaceutical Sciences, Faculty of Pharmacy and Pharmaceutical Sciences, Josai University.
[Ti] Título:p-Coumaroyl Malate Derivatives of the Pandanus amaryllifolius Leaf and Their Isomerization.
[So] Source:Chem Pharm Bull (Tokyo);65(12):1191-1194, 2017.
[Is] ISSN:1347-5223
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:A novel p-coumaroyl dimethyl malate (1) was isolated from the Pandanus amaryllifolius leaf in addition to three known analogs of p-coumaroyl dimethyl malate (2-4), and their structures were elucidated by analysis of the spectroscopic data. The p-coumaroyl malate derivatives were isolated as a mixture of E and Z isomers. To determine the cause of isomerization, the p-coumaroyl malate isolated in this study was synthesized. We concluded that the Z isomer might be an artifact generated from the E isomer through purification steps.
[Mh] Termos MeSH primário: Ácidos Cumáricos/química
Malatos/química
Pandanaceae/química
[Mh] Termos MeSH secundário: Ácidos Cumáricos/síntese química
Ácidos Cumáricos/isolamento & purificação
Espectroscopia de Ressonância Magnética
Malatos/síntese química
Malatos/isolamento & purificação
Conformação Molecular
Pandanaceae/metabolismo
Extratos Vegetais/química
Folhas de Planta/química
Folhas de Planta/metabolismo
Estereoisomerismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Coumaric Acids); 0 (Malates); 0 (Plant Extracts); 0 (p-coumaroylmalic acid)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180110
[Lr] Data última revisão:
180110
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171205
[St] Status:MEDLINE
[do] DOI:10.1248/cpb.c17-00604


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[PMID]:29173237
[Au] Autor:Robertson RC; Seira Oriach C; Murphy K; Moloney GM; Cryan JF; Dinan TG; Ross RP; Stanton C
[Ad] Endereço:1School of Microbiology,University College Cork,Cork,Republic of Ireland.
[Ti] Título:Deficiency of essential dietary n-3 PUFA disrupts the caecal microbiome and metabolome in mice.
[So] Source:Br J Nutr;118(11):959-970, 2017 Dec.
[Is] ISSN:1475-2662
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:n-3 PUFA are lipids that play crucial roles in immune-regulation, cardio-protection and neurodevelopment. However, little is known about the role that these essential dietary fats play in modulating caecal microbiota composition and the subsequent production of functional metabolites. To investigate this, female C57BL/6 mice were assigned to one of three diets (control (CON), n-3 supplemented (n3+) or n-3 deficient (n3-)) during gestation, following which their male offspring were continued on the same diets for 12 weeks. Caecal content of mothers and offspring were collected for 16S sequencing and metabolic phenotyping. n3- male offspring displayed significantly less % fat mass than n3+ and CON. n-3 Status also induced a number of changes to gut microbiota composition such that n3- offspring had greater abundance of Tenericutes, Anaeroplasma and Coriobacteriaceae. Metabolomics analysis revealed an increase in caecal metabolites involved in energy metabolism in n3+ including α-ketoglutaric acid, malic acid and fumaric acid. n3- animals displayed significantly reduced acetate, butyrate and total caecal SCFA production. These results demonstrate that dietary n-3 PUFA regulate gut microbiota homoeostasis whereby n-3 deficiency may induce a state of disturbance. Further studies are warranted to examine whether these microbial and metabolic disturbances are causally related to changes in metabolic health outcomes.
[Mh] Termos MeSH primário: Fenômenos Fisiológicos da Nutrição Animal
Ceco/microbiologia
Ácidos Graxos Ômega-3/deficiência
Microbioma Gastrointestinal
[Mh] Termos MeSH secundário: Animais
Composição Corporal
DNA Bacteriano/isolamento & purificação
Dieta
Suplementos Nutricionais
Ácidos Graxos/metabolismo
Ácidos Graxos Ômega-3/sangue
Feminino
Fumaratos/metabolismo
Ácidos Cetoglutáricos/metabolismo
Malatos/metabolismo
Masculino
Metaboloma
Metabolômica
Camundongos
Camundongos Endogâmicos C57BL
RNA Ribossômico 16S/isolamento & purificação
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Fatty Acids, Omega-3); 0 (Fumarates); 0 (Ketoglutaric Acids); 0 (Malates); 0 (RNA, Ribosomal, 16S); 817L1N4CKP (malic acid); 88XHZ13131 (fumaric acid); 8ID597Z82X (alpha-ketoglutaric acid)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171218
[Lr] Data última revisão:
171218
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171128
[St] Status:MEDLINE
[do] DOI:10.1017/S0007114517002999


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[PMID]:28850573
[Au] Autor:Korza G; Abini-Agbomson S; Setlow B; Shen A; Setlow P
[Ad] Endereço:Department of Molecular Biology and Biophysics, UConn Health, Farmington, CT, United States of America.
[Ti] Título:Levels of L-malate and other low molecular weight metabolites in spores of Bacillus species and Clostridium difficile.
[So] Source:PLoS One;12(8):e0182656, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Dormant spores of Bacillus species lack ATP and NADH and contain notable levels of only a few other common low mol wt energy reserves, including 3-phosphoglyceric acid (3PGA), and glutamic acid. Recently, Bacillus subtilis spores were reported to contain ~ 30 µmol of L-malate/g dry wt, which also could serve as an energy reserve. In present work, L-malate levels were determined in the core of dormant spores of B. subtilis, Bacillus cereus, Bacillus megaterium and Clostridium difficile, using both an enzymatic assay and 13C-NMR on extracts prepared by several different methods. These assays found that levels of L-malate in B. cereus and B. megaterium spores were ≤ 0.5 µmol/g dry wt, and ≤ 1 µmol/g dry wt in B. subtilis spores, and levels of L-lactate and pyruvate in B. megaterium and B. subtilis spores were < 0.5 µmol/g dry wt. Levels of L-malate in C. difficile spores were ≤ 1 µmol/g dry wt, while levels of 3PGA were ~ 7 µmol/g; the latter value was determined by 31P-NMR, and is in between the 3PGA levels in B. megaterium and B. subtilis spores determined previously. 13C-NMR analysis of spore extracts further showed that B. megaterium, B. subtilis and C. difficile contained significant levels of carbonate/bicarbonate in the spore core. Low mol wt carbon-containing small molecules present at > 3 µmol/g dry spores are: i) dipicolinic acid, carbonate/bicarbonate and 3PGA in B. megaterium, B. subtilis and C. difficile; ii) glutamate in B. megaterium and B. subtilis; iii) arginine in B. subtilis; and iv) at least one unidentified compound in all three species.
[Mh] Termos MeSH primário: Bacillus/metabolismo
Clostridium difficile/metabolismo
Malatos/metabolismo
Esporos Bacterianos/metabolismo
[Mh] Termos MeSH secundário: Ácido Láctico/metabolismo
Espectroscopia de Ressonância Magnética
Ácido Pirúvico/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Malates); 33X04XA5AT (Lactic Acid); 817L1N4CKP (malic acid); 8558G7RUTR (Pyruvic Acid)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171019
[Lr] Data última revisão:
171019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170830
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0182656


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[PMID]:28847084
[Au] Autor:Yiin CL; Quitain AT; Yusup S; Uemura Y; Sasaki M; Kida T
[Ad] Endereço:Biomass Processing Cluster, Center of Biofuel and Biochemical Research, Chemical Engineering Department, Mission Oriented Research (Green Technology), Universiti Teknologi PETRONAS, Bandar Seri Iskandar, 32610 Tronoh, Perak, Malaysia.
[Ti] Título:Choline chloride (ChCl) and monosodium glutamate (MSG)-based green solvents from optimized cactus malic acid for biomass delignification.
[So] Source:Bioresour Technol;244(Pt 1):941-948, 2017 Nov.
[Is] ISSN:1873-2976
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:This work aimed to develop an efficient microwave-hydrothermal (MH) extraction of malic acid from abundant natural cactus as hydrogen bond donor (HBD) whereby the concentration was optimized using response surface methodology. The ideal process conditions were found to be at a solvent-to-feed ratio of 0.008, 120°C and 20min with 1.0g of oxidant, H O . Next generation environment-friendly solvents, low transition temperature mixtures (LTTMs) were synthesized from cactus malic acid with choline chloride (ChCl) and monosodium glutamate (MSG) as hydrogen bond acceptors (HBAs). The hydrogen-bonding interactions between the starting materials were determined. The efficiency of the LTTMs in removing lignin from oil palm biomass residues, empty fruit bunch (EFB) was also evaluated. The removal of amorphous hemicellulose and lignin after the pretreatment process resulted in an enhanced digestibility and thermal degradability of biomass.
[Mh] Termos MeSH primário: Cactaceae
Malatos
Glutamato de Sódio
[Mh] Termos MeSH secundário: Biomassa
Colina
Peróxido de Hidrogênio
Hidrólise
Solventes
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Malates); 0 (Solvents); 817L1N4CKP (malic acid); BBX060AN9V (Hydrogen Peroxide); N91BDP6H0X (Choline); W81N5U6R6U (Sodium Glutamate)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171023
[Lr] Data última revisão:
171023
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170830
[St] Status:MEDLINE


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[PMID]:28780262
[Au] Autor:Xia J; Li R; He A; Xu J; Liu X; Li X; Xu J
[Ad] Endereço:Jiangsu Key Laboratory for Biomass-Based Energy and Enzyme Technology, Jiangsu Collaborative Innovation Center of Regional Modern Agriculture and Environmental Protection, College of Chemistry and Chemical Engineering, Huaiyin Normal University, Huai'an 223300, China.
[Ti] Título:Production of poly(ß-l-malic acid) by Aureobasidium pullulans HA-4D under solid-state fermentation.
[So] Source:Bioresour Technol;244(Pt 1):289-295, 2017 Nov.
[Is] ISSN:1873-2976
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Poly(ß-l-malic acid) (PMA) production by Aureobasidium pullulans HA-4D was carried out through solid-state fermentation (SSF) using agro-industrial residues. Maximum PMA production (75.4mg/g substrate) was obtained from a mixed substrate of sweet potato residue and wheat bran (1:1, w/w) supplemented with NaNO (0.8%, w/w) and CaCO (2%, w/w), with an initial moisture content of 70% and inoculum size of 13% (v/w) for 8days. Repeated-batch SSF was successfully conducted for 5 cycles with a high productivity. The scanning electron microscopy showed that the yeast-like cells of A. pullulans HA-4D could grow well on the solid substrate surface. Moreover, the cost analysis showed that the unit price of PMA in SSF was much lower than that of SmF. This is the first report on PMA production via SSF, and this study provided a new method to produce PMA from inexpensive agro-industrial residues.
[Mh] Termos MeSH primário: Fermentação
Glucanos
Malatos
[Mh] Termos MeSH secundário: Polímeros
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Glucans); 0 (Malates); 0 (Polymers); 817L1N4CKP (malic acid); 8ZQ0AYU1TT (pullulan)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171103
[Lr] Data última revisão:
171103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170807
[St] Status:MEDLINE


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[PMID]:28581736
[Au] Autor:Simó G; Vila-Crespo J; Fernández-Fernández E; Ruipérez V; Rodríguez-Nogales JM
[Ad] Endereço:Food Technology Area, University of Valladolid, Technical High School of Agronomic Engineering , Av. Madrid 44, 34071 Palencia, Spain.
[Ti] Título:Highly Efficient Malolactic Fermentation of Red Wine Using Encapsulated Bacteria in a Robust Biocomposite of Silica-Alginate.
[So] Source:J Agric Food Chem;65(25):5188-5197, 2017 Jun 28.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Bacteria encapsulation to develop malolactic fermentation emerges as a biotechnological strategy that provides significant advantages over the use of free cells. Two encapsulation methods have been proposed embedding Oenococcus oeni, (i) interpenetrated polymer networks of silica and Ca-alginate and (ii) Ca-alginate capsules coated with hydrolyzed 3-aminopropyltriethoxysilane (hAPTES). On the basis of our results, only the first method was suitable for bacteria encapsulation. The optimized silica-alginate capsules exhibited a negligible bacteria release and an increase of 328% and 65% in L-malic acid consumption and mechanical robustness, respectively, compared to untreated alginate capsules. Moreover, studies of capsule stability at different pH and ethanol concentrations in water solutions and in wine indicated a better behavior of silica-alginate capsules than untreated ones. The inclusion of silicates and colloidal silica in alginate capsules containing O. oeni improved markedly their capacity to deplete the levels of L-malic acid in red wines and their mechanical robustness and stability.
[Mh] Termos MeSH primário: Oenococcus/química
Vitis/microbiologia
Vinho/microbiologia
[Mh] Termos MeSH secundário: Alginatos/química
Células Imobilizadas/química
Fermentação
Ácido Glucurônico/química
Ácidos Hexurônicos/química
Ácido Láctico/metabolismo
Malatos/metabolismo
Oenococcus/metabolismo
Dióxido de Silício/química
Vitis/metabolismo
Vinho/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alginates); 0 (Hexuronic Acids); 0 (Malates); 33X04XA5AT (Lactic Acid); 7631-86-9 (Silicon Dioxide); 817L1N4CKP (malic acid); 8A5D83Q4RW (Glucuronic Acid); 8C3Z4148WZ (alginic acid)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170707
[Lr] Data última revisão:
170707
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170606
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b01210


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[PMID]:28542252
[Au] Autor:Boyle NR; Sengupta N; Morgan JA
[Ad] Endereço:School of Chemical Engineering, Purdue University, West Lafayette, Indiana, United States of America.
[Ti] Título:Metabolic flux analysis of heterotrophic growth in Chlamydomonas reinhardtii.
[So] Source:PLoS One;12(5):e0177292, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Despite the wealth of knowledge available for C. reinhardtii, the central metabolic fluxes of growth on acetate have not yet been determined. In this study, 13C-metabolic flux analysis (13C-MFA) was used to determine and quantify the metabolic pathways of primary metabolism in C. reinhardtii cells grown under heterotrophic conditions with acetate as the sole carbon source. Isotopic labeling patterns of compartment specific biomass derived metabolites were used to calculate the fluxes. It was found that acetate is ligated with coenzyme A in the three subcellular compartments (cytosol, mitochondria and plastid) included in the model. Two citrate synthases were found to potentially be involved in acetyl-coA metabolism; one localized in the mitochondria and the other acting outside the mitochondria. Labeling patterns demonstrate that Acetyl-coA synthesized in the plastid is directly incorporated in synthesis of fatty acids. Despite having a complete TCA cycle in the mitochondria, it was also found that a majority of the malate flux is shuttled to the cytosol and plastid where it is converted to oxaloacetate providing reducing equivalents to these compartments. When compared to predictions by flux balance analysis, fluxes measured with 13C-MFA were found to be suboptimal with respect to biomass yield; C. reinhardtii sacrifices biomass yield to produce ATP and reducing equivalents.
[Mh] Termos MeSH primário: Chlamydomonas reinhardtii/crescimento & desenvolvimento
Chlamydomonas reinhardtii/metabolismo
Processos Heterotróficos/fisiologia
[Mh] Termos MeSH secundário: Acetilcoenzima A/metabolismo
Trifosfato de Adenosina/metabolismo
Carbono/metabolismo
Isótopos de Carbono
Citrato (si)-Sintase/metabolismo
Coenzima A/metabolismo
Citosol/metabolismo
Ácidos Graxos/metabolismo
Malatos/metabolismo
Análise do Fluxo Metabólico
Mitocôndrias/metabolismo
Modelos Biológicos
Ácido Oxaloacético/metabolismo
Proteínas de Plantas/metabolismo
Plastídeos/metabolismo
Acetato de Zinco/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Carbon Isotopes); 0 (Fatty Acids); 0 (Malates); 0 (Plant Proteins); 2F399MM81J (Oxaloacetic Acid); 72-89-9 (Acetyl Coenzyme A); 7440-44-0 (Carbon); 817L1N4CKP (malic acid); 8L70Q75FXE (Adenosine Triphosphate); EC 2.3.3.1 (Citrate (si)-Synthase); FM5526K07A (Zinc Acetate); SAA04E81UX (Coenzyme A)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170919
[Lr] Data última revisão:
170919
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170526
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0177292


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[PMID]:28515085
[Au] Autor:Fink BD; Bai F; Yu L; Sivitz WI
[Ad] Endereço:Department of Internal Medicine/Endocrinology and Metabolism, University of Iowa and the Iowa City Veterans Affairs Medical Center, Iowa City, Iowa.
[Ti] Título:Regulation of ATP production: dependence on calcium concentration and respiratory state.
[So] Source:Am J Physiol Cell Physiol;313(2):C146-C153, 2017 Aug 01.
[Is] ISSN:1522-1563
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Nanomolar free calcium enhances oxidative phosphorylation. However, the effects over a broad concentration range, at different respiratory states, or on specific energy substrates are less clear. We examined the action of varying [Ca ] over respiratory states ranging 4 to 3 on skeletal muscle mitochondrial respiration, potential, ATP production, and H O production using ADP recycling to clamp external [ADP]. Calcium at 450 nM enhanced respiration in mitochondria energized by the complex I substrates, glutamate/malate (but not succinate), at [ADP] of 4-256 µM, but more substantially at intermediate respiratory states and not at all at state 4. Using varied [Ca ], we found that the stimulatory effects on respiration and ATP production were most prominent at nanomolar concentrations, but inhibitory at 10 µM or higher. ATP production decreased more than respiration at 10 µM calcium. However, potential continued to increase up to 10 µM; suggesting a calcium-induced inability to utilize potential for phosphorylation independent of opening of the mitochondrial permeability transition pore (MTP). This effect of 10 µM calcium was confirmed by direct determination of ATP production over a range of potential created by differing substrate concentrations. Consistent with past reports, nanomolar [Ca ] had a stimulatory effect on utilization of potential for phosphorylation. Increasing [Ca ] was positively and continuously associated with H O production. In summary, the stimulatory effect of calcium on mitochondrial function is substrate dependent and most prominent over intermediate respiratory states. Calcium stimulates or inhibits utilization of potential for phosphorylation dependent on concentration with inhibition at higher concentration independent of MTP opening.
[Mh] Termos MeSH primário: Trifosfato de Adenosina/biossíntese
Cálcio/metabolismo
Mitocôndrias/metabolismo
Respiração
[Mh] Termos MeSH secundário: Trifosfato de Adenosina/metabolismo
Animais
Cálcio/farmacologia
Complexo I de Transporte de Elétrons/metabolismo
Ácido Glutâmico/metabolismo
Peróxido de Hidrogênio/metabolismo
Malatos/metabolismo
Camundongos
Mitocôndrias/fisiologia
Proteínas de Transporte da Membrana Mitocondrial/metabolismo
Proteínas de Transporte da Membrana Mitocondrial/fisiologia
Fosforilação Oxidativa
Consumo de Oxigênio
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Malates); 0 (Mitochondrial Membrane Transport Proteins); 0 (mitochondrial permeability transition pore); 3KX376GY7L (Glutamic Acid); 817L1N4CKP (malic acid); 8L70Q75FXE (Adenosine Triphosphate); BBX060AN9V (Hydrogen Peroxide); EC 1.6.5.3 (Electron Transport Complex I); SY7Q814VUP (Calcium)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170814
[Lr] Data última revisão:
170814
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170519
[St] Status:MEDLINE
[do] DOI:10.1152/ajpcell.00086.2017


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[PMID]:28472722
[Au] Autor:Wikene KO; Rukke HV; Bruzell E; Tønnesen HH
[Ad] Endereço:Department of Pharmacy, School of Pharmacy, University of Oslo, P.O. Box 1068 Blindern, 0316 Oslo, Norway. Electronic address: k.o.wikene@farmasi.uio.no.
[Ti] Título:Investigation of the antimicrobial effect of natural deep eutectic solvents (NADES) as solvents in antimicrobial photodynamic therapy.
[So] Source:J Photochem Photobiol B;171:27-33, 2017 Jun.
[Is] ISSN:1873-2682
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Natural deep eutectic solvents (NADES) are a third class of liquids, separate from water and lipids. Some NADES, especially those containing organic acids, have been suggested to possess antimicrobial properties. Such properties may be advantageous when NADES are used as solvents in e.g. antimicrobial photodynamic therapy. However, to control the toxicity of acid-containing NADES, they must retain their specific qualities upon dilution. Hence, the aims of this study were to investigate the effect of dilution on the acid-containing NADES network, their antimicrobial activity on different planktonic microorganisms, and their influence on phototoxicity when used as solvents for a photosensitiser. Four bacteria and one fungus were exposed to the NADES, CS (citric acid:sucrose) and MFG (malic acid:fructose:glucose) (molar ratios 1:1 and 1:1:1, respectively), at ≤1:200 dilution. Additionally, the antimicrobial properties of the NADES were studied in Escherichia coli in terms of pH and chelating effects. In investigations of phototoxicity, the microorganisms were exposed to the photosensitiser meso-tetra(p-hydroxyphenyl)porphine (THPP; 1nM) dissolved in diluted NADES combined with blue light (27J/cm ). The eutectic network appeared to remain upon dilution ≤1:200. CS (1:200) was less toxic than an equal concentration of citric acid in the Gram-negative bacteria Klebsiella pneumoniae and E. coli (p<0.05). A higher degree of phototoxicity was induced in E. coli (~1% survival) when THPP was dissolved in CS or MFG than in phosphate buffer (~61% survival; p<0.05). No conclusion could be drawn as to whether the observed toxicity in E. coli exposed to NADES was due to the pH of the solutions or chelation of outer membrane-bound cations.
[Mh] Termos MeSH primário: Anti-Infecciosos/química
Produtos Biológicos/química
Solventes/química
[Mh] Termos MeSH secundário: Anti-Infecciosos/farmacologia
Produtos Biológicos/farmacologia
Ácido Cítrico/química
Fungos/efeitos dos fármacos
Bactérias Gram-Negativas/efeitos dos fármacos
Bactérias Gram-Negativas/efeitos da radiação
Bactérias Gram-Positivas/efeitos dos fármacos
Bactérias Gram-Positivas/efeitos da radiação
Concentração de Íons de Hidrogênio
Luz
Malatos/química
Fotoquimioterapia
Fármacos Fotossensibilizantes/química
Fármacos Fotossensibilizantes/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Infective Agents); 0 (Biological Products); 0 (Malates); 0 (Photosensitizing Agents); 0 (Solvents); 2968PHW8QP (Citric Acid); 817L1N4CKP (malic acid)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170920
[Lr] Data última revisão:
170920
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE



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