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Pesquisa : D02.241.081.337.463.703 [Categoria DeCS]
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  1 / 211 MEDLINE  
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[PMID]:28358882
[Au] Autor:Meinert C; Brandt U; Heine V; Beyert J; Schmidl S; Wübbeler JH; Voigt B; Riedel K; Steinbüchel A
[Ad] Endereço:Institut für Molekulare Mikrobiologie und Biotechnologie, Westfälische Wilhelms-Universität, Münster, Germany.
[Ti] Título:Proteomic analysis of organic sulfur compound utilisation in Advenella mimigardefordensis strain DPN7T.
[So] Source:PLoS One;12(3):e0174256, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:2-Mercaptosuccinate (MS) and 3,3´-ditiodipropionate (DTDP) were discussed as precursor substance for production of polythioesters (PTE). Therefore, degradation of MS and DTDP was investigated in Advenella mimigardefordensis strain DPN7T, applying differential proteomic analysis, gene deletion and enzyme assays. Protein extracts of cells cultivated with MS, DTDP or 3-sulfinopropionic acid (SP) were compared with those cultivated with propionate (P) and/or succinate (S). The chaperone DnaK (ratio DTDP/P 9.2, 3SP/P 4.0, MS/S 6.1, DTDP/S 6.2) and a Do-like serine protease (DegP) were increased during utilization of all organic sulfur compounds. Furthermore, a putative bacterioferritin (locus tag MIM_c12960) showed high abundance (ratio DTDP/P 5.3, 3SP/P 3.2, MS/S 4.8, DTDP/S 3.9) and is probably involved in a thiol-specific stress response. The deletion of two genes encoding transcriptional regulators (LysR (MIM_c31370) and Xre (MIM_c31360)) in the close proximity of the relevant genes of DTDP catabolism (acdA, mdo and the genes encoding the enzymes of the methylcitric acid cycle; prpC,acnD, prpF and prpB) showed that these two regulators are essential for growth of A. mimigardefordensis strain DPN7T with DTDP and that they most probably regulate transcription of genes mandatory for this catabolic pathway. Furthermore, proteome analysis revealed a high abundance (ratio MS/S 10.9) of a hypothetical cupin-2-domain containing protein (MIM_c37420). This protein shows an amino acid sequence similarity of 60% to a newly identified MS dioxygenase from Variovorax paradoxus strain B4. Deletion of the gene and the adjacently located transcriptional regulator LysR, as well as heterologous expression of MIM_c37420, the putative mercaptosuccinate dioxygenase (Msdo) from A. mimigardefordensis, showed that this protein is the key enzyme of MS degradation in A. mimigardefordensis strain DPN7T (KM 0.2 mM, specific activity 17.1 µmol mg-1 min-1) and is controlled by LysR (MIM_c37410).
[Mh] Termos MeSH primário: Alcaligenaceae/metabolismo
Compostos Orgânicos/metabolismo
Proteômica/métodos
[Mh] Termos MeSH secundário: Eletroforese em Gel Bidimensional
Espectrometria de Massas
Propionatos/metabolismo
Proteoma/metabolismo
Software
Tiomalatos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Organic Chemicals); 0 (Propionates); 0 (Proteome); 0 (Thiomalates); 52329-65-4 (3-sulfinopropionic acid); 94239W5L4H (2-thiomalic acid)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170828
[Lr] Data última revisão:
170828
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170331
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0174256


  2 / 211 MEDLINE  
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[PMID]:27398533
[Au] Autor:Chen N; Chen J; Yang JH; Bai LY; Zhang YP
[Ti] Título:Colorimetric Detection of Cadmium Ions Using DL-Mercaptosuccinic Acid-Modified Gold Nanoparticles.
[So] Source:J Nanosci Nanotechnol;16(1):840-3, 2016 Jan.
[Is] ISSN:1533-4880
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A colorimetric assay has been developed for detection of Cd²âº utilizing DL-mercaptosuccinic acid-modified gold nanoparticles (MSA-AuNPs). The method showed good selectivity for Cd²âº over other metal ions. As a result, the linear relationships (r > 0.9606) between concentration 0.07 mM and 0.20 mM for cadmium ion were obtained. The detection limit was as low as 0.07 mM by the naked eye. The effect of pH on the aggregation was optimized. The MSA-AuNPs probe could be used to detect Cd²âº in an aqueous solution based on the aggregation-induced color change of MSA-AuNPs.
[Mh] Termos MeSH primário: Cádmio/análise
Ouro/química
Nanopartículas Metálicas/química
Tiomalatos/química
[Mh] Termos MeSH secundário: Íons/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Ions); 0 (Thiomalates); 00BH33GNGH (Cadmium); 7440-57-5 (Gold); 94239W5L4H (2-thiomalic acid)
[Em] Mês de entrada:1608
[Cu] Atualização por classe:160711
[Lr] Data última revisão:
160711
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160712
[St] Status:MEDLINE


  3 / 211 MEDLINE  
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[PMID]:27151673
[Au] Autor:Bao L; Zhu X; Dai H; Tao Y; Zhou X; Liu W; Kong Y
[Ad] Endereço:Advanced Catalysis and Green Manufacturing Collaborative Innovation Center, School of Petrochemical Engineering, Changzhou University, Changzhou 213164, China.
[Ti] Título:Synthesis of porous starch xerogels modified with mercaptosuccinic acid to remove hazardous gardenia yellow.
[So] Source:Int J Biol Macromol;89:389-95, 2016 Aug.
[Is] ISSN:1879-0003
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Mercaptosuccinic acid (MSA) molecules were inserted into potato starch, leading to the breaking of intrinsic H-bonds within macromolecular chains of starch and the formation of intermolecular H-bonds between MSA and starch, which could be verified by Fourier transform infrared spectroscopy (FT-TR). MSA modified porous starch xerogels (PSX/MSA) were obtained after freeze-drying the MSA modified starch, and they were characterized by field emission scanning electron microscopy (FESEM), exhibiting the intriguing porous structure due to the separation of starch chains by MSA molecules. The PSX/MSA were then used as the adsorbents to remove gardenia yellow (GY), a natural colorant with genotoxicity. Due to the porous structure of PSX and the introduced carboxyl groups from MSA, the adsorption capacity of the PSX/MSA was much higher than that of the starch xerogels alone (SX). The adsorption behaviors of GY by the PSX/MSA fitted both the Freundlich isotherm model and the pseudo-second-order kinetic model, and the efficient adsorption of GY suggested that the PSX/MSA might be potential adsorbents for the removal of dyes from contaminated aquatic systems.
[Mh] Termos MeSH primário: Corantes/química
Gardenia/química
Extratos Vegetais/química
Amido/química
Tiomalatos/química
[Mh] Termos MeSH secundário: Adsorção
Corantes/toxicidade
Gardenia/toxicidade
Ligações de Hidrogênio/efeitos dos fármacos
Cinética
Microscopia Eletrônica de Varredura
Extratos Vegetais/toxicidade
Porosidade
Espectroscopia de Infravermelho com Transformada de Fourier
Amido/farmacologia
Tiomalatos/farmacologia
Poluentes Químicos da Água/química
Poluentes Químicos da Água/toxicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Coloring Agents); 0 (Plant Extracts); 0 (Thiomalates); 0 (Water Pollutants, Chemical); 9005-25-8 (Starch); 94239W5L4H (2-thiomalic acid); F32BA2H92Z (gardenia yellow)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160507
[St] Status:MEDLINE


  4 / 211 MEDLINE  
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[PMID]:26893560
[Au] Autor:Yan M; Zhang Y; Qin H; Liu K; Guo M; Ge Y; Xu M; Sun Y; Zheng X
[Ad] Endereço:Department of Biomedical Engineering, College of Life Information Science and Instrument Engineering, Hangzhou Dianzi University, Hangzhou, People's Republic of China.
[Ti] Título:Cytotoxicity of CdTe quantum dots in human umbilical vein endothelial cells: the involvement of cellular uptake and induction of pro-apoptotic endoplasmic reticulum stress.
[So] Source:Int J Nanomedicine;11:529-42, 2016.
[Is] ISSN:1178-2013
[Cp] País de publicação:New Zealand
[La] Idioma:eng
[Ab] Resumo:Cadmium telluride quantum dots (CdTe QDs) have been proposed to induce oxidative stress, which plays a crucial role in CdTe QDs-mediated mitochondrial-dependent apoptosis in human umbilical vein endothelial cells (HUVECs). However, the direct interactions of CdTe QDs with HUVECs and their potential impairment of other organelles like endoplasmic reticulum (ER) in HUVECs are poorly understood. In this study, we reported that the negatively charged CdTe QDs (-21.63±0.91 mV), with good dispersity and fluorescence stability, were rapidly internalized via endocytosis by HUVECs, as the notable internalization could be inhibited up to 95.52% by energy depletion (NaN3/deoxyglucose or low temperature). The endocytosis inhibitors (methyl-ß-cyclodextrin, genistein, sucrose, chlorpromazine, and colchicine) dramatically decreased the uptake of CdTe QDs by HUVECs, suggesting that both caveolae/raft- and clathrin-mediated endocytosis were involved in the endothelial uptake of CdTe QDs. Using immunocytochemistry, a striking overlap of the internalized CdTe QDs and ER marker was observed, which indicates that QDs may be transported to ER. The CdTe QDs also caused remarkable ER stress responses in HUVECs, confirmed by significant dilatation of ER cisternae, upregulation of ER stress markers GRP78/GRP94, and activation of protein kinase RNA-like ER kinase-eIF2α-activating transcription factor 4 pathway (including phosphorylation of both protein kinase RNA-like ER kinase and eIF2α and elevated level of activating transcription factor 4). CdTe QDs further promoted an increased C/EBP homologous protein expression, phosphorylation of c-JUN NH2-terminal kinase, and cleavage of ER-resident caspase-4, while the specific inhibitor (SP600125, Z-LEVD-fmk, or salubrinal) significantly attenuated QDs-triggered apoptosis, indicating that all three ER stress-mediated apoptosis pathways were activated and the direct participation of ER in the CdTe QDs-caused apoptotic cell death in HUVECs. Our findings provide important new insights into QDs toxicity and reveal potential cardiovascular risks for the future applications of QDs.
[Mh] Termos MeSH primário: Compostos de Cádmio/farmacologia
Estresse do Retículo Endoplasmático/efeitos dos fármacos
Pontos Quânticos
Telúrio/farmacologia
[Mh] Termos MeSH secundário: Apoptose/efeitos dos fármacos
Apoptose/fisiologia
Compostos de Cádmio/química
Colchicina/farmacologia
Endocitose/efeitos dos fármacos
Fluorescência
Genisteína/farmacologia
Proteínas de Choque Térmico/metabolismo
Células Endoteliais da Veia Umbilical Humana/efeitos dos fármacos
Células Endoteliais da Veia Umbilical Humana/metabolismo
Seres Humanos
Pontos Quânticos/química
Telúrio/química
Tiomalatos/química
beta-Ciclodextrinas/farmacologia
eIF-2 Quinase/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Cadmium Compounds); 0 (Heat-Shock Proteins); 0 (Thiomalates); 0 (beta-Cyclodextrins); 0 (methyl-beta-cyclodextrin); 0 (molecular chaperone GRP78); 94239W5L4H (2-thiomalic acid); DH2M523P0H (Genistein); EC 2.7.11.1 (eIF-2 Kinase); NQA0O090ZJ (Tellurium); SML2Y3J35T (Colchicine); STG188WO13 (cadmium telluride)
[Em] Mês de entrada:1609
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160220
[St] Status:MEDLINE
[do] DOI:10.2147/IJN.S93591


  5 / 211 MEDLINE  
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[PMID]:26743581
[Au] Autor:Liang X; Wang H; Zhu Y; Zhang R; Cogger VC; Liu X; Xu ZP; Grice JE; Roberts MS
[Ad] Endereço:Therapeutics Research Centre, School of Medicine, The University of Queensland , Princess Alexandra Hospital, Woolloongabba, QLD 4102, Australia.
[Ti] Título:Short- and Long-Term Tracking of Anionic Ultrasmall Nanoparticles in Kidney.
[So] Source:ACS Nano;10(1):387-95, 2016 Jan 26.
[Is] ISSN:1936-086X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:While biodistribution of nanoparticles (NPs) has been widely studied at the organ level, relatively little is known about their disposition in organs at the cellular level, especially after long-term exposure. The kidney is regarded as the key organ for the clearance of ultrasmall NPs (<5.5 nm). However, recent studies indicate that NPs in this size range could accumulate in the kidney for extended times without urinary excretion. Using negatively charged quantum dots (QDs) (∼3.7 nm) as a model system, we examined the suborgan disposition of anionic ultrasmall NPs in the kidney at the cellular level after intravenous injection by multiphoton microscopy coupled with fluorescence lifetime imaging. Most of the NPs were initially distributed in the peritubular capillaries or glomerular arterioles after injection, whereas they passed through the fenestrated glomerular endothelium and were gradually taken up by mesangial cells up to 30 days after injection. Only trace amounts of anionic QDs could be detected in the urine, which could be attributed to the barrier of the anionic glomerular basement membrane preventing filtration of anionic QDs. In contrast, cationic QDs of similar size (∼5.67 nm) were found to be readily excreted into urine. This study thus highlights the importance of surface charge in determining renal clearance of ultrasmall NPs. It provides a framework for characterizing and predicting the subcellular disposition in organs and long-term targeting of other NPs, with a physiologically based kinetic model being subsequently developed to describe the suborgan kinetics of anionic ultrasmall NPs.
[Mh] Termos MeSH primário: Membrana Basal Glomerular/efeitos dos fármacos
Células Mesangiais/efeitos dos fármacos
Nanopartículas/metabolismo
Polietilenoimina/química
Tiomalatos/química
[Mh] Termos MeSH secundário: Animais
Ânions
Cátions
Membrana Basal Glomerular/metabolismo
Taxa de Filtração Glomerular
Células Mesangiais/metabolismo
Camundongos
Camundongos Endogâmicos BALB C
Microscopia de Fluorescência por Excitação Multifotônica
Imagem Óptica
Tamanho da Partícula
Pontos Quânticos/química
Soroalbumina Bovina/química
Eletricidade Estática
Distribuição Tecidual
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anions); 0 (Cations); 0 (Thiomalates); 27432CM55Q (Serum Albumin, Bovine); 9002-98-6 (Polyethyleneimine); 94239W5L4H (2-thiomalic acid)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160109
[St] Status:MEDLINE
[do] DOI:10.1021/acsnano.5b05066


  6 / 211 MEDLINE  
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[PMID]:26301311
[Au] Autor:Rekha MR; Sharma CP
[Ti] Título:Simultaneous Effect of Thiolation and Carboxylation of Chitosan Particles Towards Mucoadhesive Oral Insulin Delivery Applications: An In Vitro and In Vivo Evaluation.
[So] Source:J Biomed Nanotechnol;11(1):165-76, 2015 Jan.
[Is] ISSN:1550-7033
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Thiomalyl chitosan (TCS), a pH sensitive thiolated chitosan derivative, was developed and investigated towards oral protein delivery application. Particles of z-average 364 ± 5.6 nm with a negative zeta potential of 14.4 mV was obtained by tripolyphosphate cross linking of TCS. The release of insulin from TCS particles was significantly restricted at pH 1.2 minimizing up to about < 10% in 3 hours. The permeation enhancement ratio was found to 13 times higher than the FD4 alone and was 1.6 times higher than the unmodified chitosan particles. The protein protective properties of the matrix were established in presence of pepsin and pancreatic enzymes. Confocal microscopy studies proved the tight junction opening of Caco-2 cells by these thiolated chitosan particles and the in vivo studies on diabetic rats established its potential towards oral peptide delivery with pharmacological availability (PA) of 1.5%. The significance of this work is to establish that, the presence of multiple functional groups having similar property in the same matrix can improve its suitability as a promising candidate for oral peptide delivery with improved release characteristics, mucoadhesion as well as protecting the insulin activity and enhancing the permeability across the intestinal wall.
[Mh] Termos MeSH primário: Quitosana/química
Preparações de Ação Retardada/química
Diabetes Mellitus Experimental/tratamento farmacológico
Mucinas Gástricas/química
Insulina/química
Nanocápsulas/química
[Mh] Termos MeSH secundário: Administração Oral
Animais
Glicemia/análise
Células CACO-2
Preparações de Ação Retardada/administração & dosagem
Diabetes Mellitus Experimental/sangue
Diabetes Mellitus Experimental/induzido quimicamente
Difusão
Seres Humanos
Insulina/administração & dosagem
Masculino
Nanocápsulas/administração & dosagem
Nanocápsulas/ultraestrutura
Tamanho da Partícula
Ratos
Ratos Wistar
Estreptozocina
Tiomalatos/química
Resultado do Tratamento
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Blood Glucose); 0 (Delayed-Action Preparations); 0 (Gastric Mucins); 0 (Insulin); 0 (Nanocapsules); 0 (Thiomalates); 5W494URQ81 (Streptozocin); 9012-76-4 (Chitosan)
[Em] Mês de entrada:1509
[Cu] Atualização por classe:150824
[Lr] Data última revisão:
150824
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150825
[St] Status:MEDLINE


  7 / 211 MEDLINE  
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[PMID]:25936377
[Au] Autor:Brandt U; Deters A; Steinbüchel A
[Ad] Endereço:Institut für Molekulare Mikrobiologie und Biotechnologie, Westfälische Wilhelms-Universität, Corrensstraße 3, D-48149, Münster, Germany.
[Ti] Título:A jack-of-all-trades: 2-mercaptosuccinic acid.
[So] Source:Appl Microbiol Biotechnol;99(11):4545-57, 2015 Jun.
[Is] ISSN:1432-0614
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:2-Mercaptosuccinic acid (MS) is an important and versatile substance for diverse fields of applications of which the most significant are surveyed in this article. Biological, chemical, and physical properties of MS as well as the knowledge of its synthesis and microbial degradation are illustrated. In addition, exemplary structural analogs of the organic sulfur compound are commented. The key application of MS in nanotechnology is discussed in detail with particular emphasis on quantum dots (nanocrystals) and self-assembled monolayers in combination with gold or silver. Furthermore, some medical and pharmaceutical applications are given, inter alia in bioimaging, as a nanocarrier, and with regard to the antimicrobial activity of MS-silver and MS-gold nanoparticles. Moreover, biological and chemical applications of MS are exemplified: the thiol compound can serve as an inhibitor for glutathione peroxidase, or the toxicity of substances can be increased due to the presence of MS in the respective cells or tissues. In the field of cosmetics, MS is widely utilized as a reducing agent for numerous products as explained in this article. Additionally, the microbial utilization of MS as a carbon and energy source for growth is elucidated in-depth, providing insight into different catabolic mechanisms.
[Mh] Termos MeSH primário: Nanotecnologia/métodos
Tiomalatos/metabolismo
[Mh] Termos MeSH secundário: Tecnologia Biomédica/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Thiomalates); 94239W5L4H (2-thiomalic acid)
[Em] Mês de entrada:1602
[Cu] Atualização por classe:150518
[Lr] Data última revisão:
150518
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150505
[St] Status:MEDLINE
[do] DOI:10.1007/s00253-015-6605-2


  8 / 211 MEDLINE  
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[PMID]:25907598
[Au] Autor:Schanuel FS; Raggio Santos KS; Monte-Alto-Costa A; de Oliveira MG
[Ad] Endereço:Histology and Embryology Department, State University of Rio de Janeiro, UERJ, Rio de Janeiro 20950-003, Brazil.
[Ti] Título:Combined nitric oxide-releasing poly(vinyl alcohol) film/F127 hydrogel for accelerating wound healing.
[So] Source:Colloids Surf B Biointerfaces;130:182-91, 2015 Jun 01.
[Is] ISSN:1873-4367
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Nitric oxide (NO) releasing biomaterials represent a potential strategy for use as active wound dressings capable of accelerating wound healing. Topical NO-releasing poly(vinyl alcohol) (PVA) films and Pluronic F127 hydrogels (F127) have already exhibited effective skin vasodilation and wound healing actions. In this study, we functionalized PVA films with SNO groups via esterification with a mixture of mercaptosucinic acid (MSA) and thiolactic acid (TLA) followed by S-nitrosation of the SH moieties. These films were combined with an underlying layer of poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide), i.e., PEO-PPO-PEO (Pluronic F127) hydrogel and used for the topical treatment of skin lesions in an animal model. The mixed esterification of PVA with MSA and TLA led to chemically crosslinked PVA-SNO films with a high swelling capacity capable of spontaneously releasing NO. Real time NO-release measurements revealed that the hydrogel layer reduces the initial NO burst from the PVA-SNO films. We demonstrate that the combination of PVA-SNO films with F127 hydrogel accelerates wound contraction, decreases wound gap and cellular density and accelerates the inflammatory phase of the lesion. These results were reflected in an increase in myofibroblastic differentiation and collagen type III expression in the cicatricial tissue. Therefore, PVA-SNO films combined with F127 hydrogel may represent a new approach for active wound dressings capable of accelerating wound healing.
[Mh] Termos MeSH primário: Hidrogéis/química
Óxido Nítrico/química
Poloxâmero/química
Álcool de Polivinil/química
[Mh] Termos MeSH secundário: Actinas/metabolismo
Animais
Antígenos de Diferenciação/metabolismo
Western Blotting
Hidrogéis/metabolismo
Hidrogéis/farmacologia
Imuno-Histoquímica
Masculino
Camundongos
Óxido Nítrico/metabolismo
Poloxâmero/metabolismo
Polietilenoglicóis/química
Polietilenoglicóis/metabolismo
Álcool de Polivinil/metabolismo
Álcool de Polivinil/farmacologia
Propilenoglicóis/química
Propilenoglicóis/metabolismo
S-Nitrosoglutationa/química
S-Nitrosoglutationa/metabolismo
Pele/metabolismo
Pele/patologia
Pele/fisiopatologia
Compostos de Sulfidrila/química
Tiomalatos/química
Fatores de Tempo
Cicatrização/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Actins); 0 (Antigens, Differentiation); 0 (Hydrogels); 0 (PEO-PPO-PEO); 0 (Propylene Glycols); 0 (Sulfhydryl Compounds); 0 (Thiomalates); 0 (monocyte-macrophage differentiation antigen); 106392-12-5 (Poloxamer); 30IQX730WE (Polyethylene Glycols); 31C4KY9ESH (Nitric Oxide); 57564-91-7 (S-Nitrosoglutathione); 9002-89-5 (Polyvinyl Alcohol); 94239W5L4H (2-thiomalic acid); O5U6967KGF (thiolactic acid)
[Em] Mês de entrada:1604
[Cu] Atualização por classe:150605
[Lr] Data última revisão:
150605
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150425
[St] Status:MEDLINE


  9 / 211 MEDLINE  
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[PMID]:25609948
[Au] Autor:Lin G; Wang X; Yin F; Yong KT
[Ad] Endereço:The Engineering Lab of Synthetic Biology and Research Institute of Uropoiesis and Reproduction, School of Medicine, Shenzhen University, Shenzhen, People's Republic of China.
[Ti] Título:Passive tumor targeting and imaging by using mercaptosuccinic acid-coated near-infrared quantum dots.
[So] Source:Int J Nanomedicine;10:335-45, 2015.
[Is] ISSN:1178-2013
[Cp] País de publicação:New Zealand
[La] Idioma:eng
[Ab] Resumo:In this paper, we demonstrate the preparation of monodispersed quantum dots (QDs) as near-infrared (NIR) optical probes for in vivo pancreatic cancer targeting and imaging. The design of these luminescent probes involves functionalizing NIR QDs with ligand mercaptosuccinic acid (MSA), which targets the tumor site by enhanced permeability and retention effect. The colloidal and optical stability of the QDs can be maintained for >1 week. In vivo optical imaging studies in nude mice bearing pancreatic tumor show that the probes accumulate at tumor sites for >2.5 hours following intravenous injection of the functionalized NIR QDs. Tumor-labeling studies showed no evidence of harmful effects on the treated animals, even at a dose as high a ~50 mg/kg. These results demonstrate that the engineered MSA-functionalized QDs can serve as a diagnostic platform for early detection of cancer, as well as in image-guided precise surgical resection of tumors.
[Mh] Termos MeSH primário: Substâncias Luminescentes
Neoplasias Experimentais/patologia
Imagem Óptica/métodos
Pontos Quânticos
Tiomalatos
[Mh] Termos MeSH secundário: Animais
Substâncias Luminescentes/química
Substâncias Luminescentes/uso terapêutico
Camundongos
Camundongos Nus
Pontos Quânticos/química
Pontos Quânticos/uso terapêutico
Tiomalatos/química
Tiomalatos/uso terapêutico
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Luminescent Agents); 0 (Thiomalates); 94239W5L4H (2-thiomalic acid)
[Em] Mês de entrada:1604
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150123
[St] Status:MEDLINE
[do] DOI:10.2147/IJN.S74805


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[PMID]:25559489
[Au] Autor:Tenório DP; Andrade CG; Cabral Filho PE; Sabino CP; Kato IT; Carvalho LB; Alves S; Ribeiro MS; Fontes A; Santos BS
[Ad] Endereço:Departamento de Ciências Farmacêuticas, Universidade Federal de Pernambuco, Recife, PE, Brazil.
[Ti] Título:CdTe quantum dots conjugated to concanavalin A as potential fluorescent molecular probes for saccharides detection in Candida albicans.
[So] Source:J Photochem Photobiol B;142:237-43, 2015 Jan.
[Is] ISSN:1873-2682
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Semiconductor colloidal quantum dots (QDs) have been applied in biological analysis due to their unique optical properties and their versatility to be conjugated to biomolecules, such as lectins and antibodies, acquiring specificity to label a variety of targets. Concanavalin A (Con A) lectin binds specifically to α-d-mannose and α-d-glucose regions of saccharides that are usually expressed on membranes of mammalian cells and on cell walls of microbials. Candida albicans is the most common fungal opportunistic pathogen present in humans. Therefore, in this work, this fungus was chosen as a model for understanding cells and biofilm-forming organisms. Here, we report an efficient bioconjugation process to bind CdTe (Cadmium Telluride) QDs to Con A, and applied the bioconjugates to label saccharide structures on the cellular surface of C. albicans suspensions and biofilms. By accomplishing hemagglutination experiments and circular dichroism, we observed that the Con A structure and biochemical properties were preserved after the bioconjugation. Fluorescence microscopy images of yeasts and hyphae cells, as well as biofilms, incubated with QDs-(Con A) showed a bright orange fluorescence profile, indicating that the cell walls were specifically labeled. Furthermore, flow cytometry measurements confirmed that over 93% of the yeast cells were successfully labeled by QD-(Con A) complex. In contrast, non-conjugated QDs or QDs-(inhibited Con A) do not label any kind of biological system tested, indicating that the bioconjugation was specific and efficient. The staining pattern of the cells and biofilms demonstrate that QDs were effectively bioconjugated to Con A with specific labeling of saccharide-rich structures on C. albicans. Consequently, this work opens new possibilities to monitor glucose and mannose molecules through fluorescence techniques, which can help to optimize phototherapy protocols for this kind of fungus.
[Mh] Termos MeSH primário: Candida albicans/metabolismo
Concanavalina A/química
Corantes Fluorescentes/química
Glucose/análise
Manose/análise
Pontos Quânticos/química
Espectrometria de Fluorescência
[Mh] Termos MeSH secundário: Compostos de Cádmio/química
Concanavalina A/metabolismo
Microscopia de Fluorescência
Telúrio/química
Tiomalatos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Cadmium Compounds); 0 (Fluorescent Dyes); 0 (Thiomalates); 11028-71-0 (Concanavalin A); 94239W5L4H (2-thiomalic acid); IY9XDZ35W2 (Glucose); NQA0O090ZJ (Tellurium); PHA4727WTP (Mannose); STG188WO13 (cadmium telluride)
[Em] Mês de entrada:1509
[Cu] Atualização por classe:161125
[Lr] Data última revisão:
161125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150107
[St] Status:MEDLINE



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