Base de dados : MEDLINE
Pesquisa : D02.241.081.337.864 [Categoria DeCS]
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  1 / 1948 MEDLINE  
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[PMID]:29337668
[Au] Autor:Badawi AA; Hegazy MM; Louis D; Eldegwy MA
[Ad] Endereço:1Department of Pharmaceutics and Industrial Pharmacy Faculty of Pharmacy, Cairo University Cairo, Egypt.
[Ti] Título:Solving manufacturing problems for L-carnitine-L-tartrate to improve the likelihood of successful product scale-up.
[So] Source:Acta Pharm;67(4):511-525, 2017 Dec 20.
[Is] ISSN:1846-9558
[Cp] País de publicação:Croatia
[La] Idioma:eng
[Ab] Resumo:L-carnitine-L-tartrate, a non-essential amino acid, is hygroscopic. This causes a problem in tablet production due to pronounced adhesion of tablets to punches. A 33 full factorial design was adopted to suggest a tablet formulation. Three adsorbents were suggested (Aerosil 200, Aerosil R972, talc) to reduce stickiness at three concentrations (1, 3 and 5 %), and three fillers (mannitol, Avicel PH 101, Dibasic calcium phosphate) were chosen to prepare 27 formulations. Micromeritic properties of formulations were studied, and tablets were prepared by wet granulation. Absence of picking, sticking or capping, recording of sufficient hardness, acceptable friability and tablet ejection force indicated formulation success. The resulting formulation prepared using Avicel PH 101 and 1 % Aerosil 200 was submitted to further investigation in order to choose the most suitable compression conditions using a 33 full factorial design. Variables included compression force, tableting rate and magnesium stearate (lubricant) concentration. The formulation prepared at compression force of 25 kN, using 2 % magnesium stearate, at a production rate of 30 tablets/ minute, was found to be the most appropriate scale up candidate.
[Mh] Termos MeSH primário: Carnitina/síntese química
Comprimidos/síntese química
Tartaratos/síntese química
[Mh] Termos MeSH secundário: Química Farmacêutica/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Tablets); 0 (Tartrates); S7UI8SM58A (Carnitine); W4888I119H (tartaric acid)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180131
[Lr] Data última revisão:
180131
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180117
[St] Status:MEDLINE


  2 / 1948 MEDLINE  
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[PMID]:28450170
[Au] Autor:Choi JS; Kwon SH; Lee SE; Jang WS; Byeon JC; Jeong HM; Park JS
[Ad] Endereço:College of Pharmacy and Institute of Drug Research and Development, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 34134, South Korea.
[Ti] Título:Use of acidifier and solubilizer in tadalafil solid dispersion to enhance the in vitro dissolution and oral bioavailability in rats.
[So] Source:Int J Pharm;526(1-2):77-87, 2017 Jun 30.
[Is] ISSN:1873-3476
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The purpose of this study is to improve the solubility, in vitro dissolution, and oral bioavailability in rats of tadalafil (TDF) by using SD technique with a weak acid and a copolymer. TDF-SD was prepared via solvent evaporation, coupled with the incorporation of an acidifier and solubilizer. Tartaric acid enhanced the solubility of TDF over 5-fold in DW, and Soluplus enhanced the solubility of TDF over 8.7-fold and 19.2-fold compared to that of TDF (pure) in DW and pH 1.2 for 1h, respectively. The optimal formulation of TDF-SD3 was composed of TDF vs Tartaric acid vs Soluplus vs Aerosil=1:1:3:3. The in vitro dissolution rate of TDF-SD3 in DW, pH 1.2 and pH 6.8 buffer (51.5%, 53.3%, and 33.2%, respectively) was significantly higher than that of the commercial product (Cialis ) powder (16.5%, 15.2%, and 14.8%, respectively). TDF was completely transformed to an amorphous form as shown in SEM, DSC and PXRD data. The stability of TDF-SD3 included drug contents and in vitro dissolution for 1 month were similar to those of Cialis , and the amorphous form of TDF-SD3 was well maintained for 6 months. The TDF-SD3 formulation improved the relative bioavailability (BA) and peak plasma concentration (C ) compared to that of Cialis powder after oral administration in rats as 117.3% and 135.7%, respectively. From the results, we found that the acidifier increased the wettability of TDF, and the solubilizer improved solubility through hydrogen bonding with TDF, thereby increasing the solubility, dissolution and oral bioavailability of TDF in TDF-SD3.
[Mh] Termos MeSH primário: Disponibilidade Biológica
Tadalafila/química
Tadalafila/farmacocinética
[Mh] Termos MeSH secundário: Administração Oral
Animais
Células CACO-2
Química Farmacêutica
Composição de Medicamentos
Seres Humanos
Masculino
Polietilenoglicóis/química
Polivinil/química
Ratos
Ratos Sprague-Dawley
Solubilidade
Tartaratos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Polyvinyls); 0 (Tartrates); 0 (polyvinyl caprolactam-polyvinyl acetate-polyethylene glycol graft copolymer); 30IQX730WE (Polyethylene Glycols); 742SXX0ICT (Tadalafil); W4888I119H (tartaric acid)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180104
[Lr] Data última revisão:
180104
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE


  3 / 1948 MEDLINE  
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[PMID]:28722413
[Au] Autor:Buckler JN; Meek T; Banwell MG; Carr PD
[Ad] Endereço:Research School of Chemistry, Institute of Advanced Studies, The Australian National University , Canberra, ACT 2601, Australia.
[Ti] Título:Total Synthesis of the Cyclic Carbonate-Containing Natural Product Aspergillusol B from d-(-)-Tartaric Acid.
[So] Source:J Nat Prod;80(7):2088-2093, 2017 Jul 28.
[Is] ISSN:1520-6025
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A total synthesis of compound 3 from d-(-)-tartaric acid is reported, thereby establishing that the structure, including relative stereochemistry, originally assigned to the cyclic carbonate-containing natural product aspergillusol B is correct.
[Mh] Termos MeSH primário: Carbonatos/química
Tartaratos/química
Tirosina/análogos & derivados
Tirosina/síntese química
[Mh] Termos MeSH secundário: Produtos Biológicos/química
Estrutura Molecular
Ressonância Magnética Nuclear Biomolecular
Estereoisomerismo
Tirosina/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biological Products); 0 (Carbonates); 0 (Tartrates); 0 (aspergillusol B); 42HK56048U (Tyrosine); W4888I119H (tartaric acid)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170915
[Lr] Data última revisão:
170915
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170720
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jnatprod.7b00303


  4 / 1948 MEDLINE  
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[PMID]:28571874
[Au] Autor:Hu X; He M
[Ad] Endereço:State Key Laboratory of Water Environment Simulation, School of Environment, Beijing Normal University, Beijing 100875, China; State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, University of Chinese Academy of Sciences, Beijing 100085, China. Electronic address: xyhu@rcees.ac.cn.
[Ti] Título:Organic ligand-induced dissolution kinetics of antimony trioxide.
[So] Source:J Environ Sci (China);56:87-94, 2017 Jun.
[Is] ISSN:1001-0742
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The influence of low-molecular-weight dissolved organic matter (LMWDOM) on the dissolution rate of Sb O was investigated. Some representative LMWDOMs with carboxyl, hydroxyl, hydrosulfuryl and amidogen groups occurring naturally in the solution were chosen, namely oxalic acid, citric acid, tartaric acid, EDTA, salicylic acid, phthalandione, glycine, thiolactic acid, xylitol, glucose and catechol. These LMWDOMs were dissolved in inert buffers at pH=3.7, 6.6 and 8.6 and added to powdered Sb O in a stirred, thermostatted reactor (25°C). The addition of EDTA, tartaric acid, thiolactic acid, citric acid and oxalic acid solutions at pH3.7 and catechol at pH8.6 increased the rate of release of antimony. In the 10mmol/L thiolactic acid solution, up to 97% by mass of the antimony was released after 120min reaction. There was no effect on the dissolution of Sb O for the other ligands. A weak correlation between dissolution rate with the dissociation constant of ligands and the stability of the dissolved complex was also found. All the results showed that the extent of the promoting effect of ligands on the dissolution of Sb O was not determined by the stability of the dissolved complex, but by the dissociation constant of ligands and detachment rate of surface chelates from the mineral surface. This study can not only help in further understanding the effect of individual low-molecular-weight organic ligands, but also provides a reference to deduce the effect of natural organic matters with oxygen-bearing functional groups on the dissolution of antimony oxide minerals.
[Mh] Termos MeSH primário: Antimônio/química
Modelos Químicos
[Mh] Termos MeSH secundário: Quelantes
Ácido Cítrico
Concentração de Íons de Hidrogênio
Cinética
Ligantes
Minerais
Ácido Oxálico
Óxidos
Solubilidade
Soluções
Tartaratos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Chelating Agents); 0 (Ligands); 0 (Minerals); 0 (Oxides); 0 (Solutions); 0 (Tartrates); 1332-81-6 (antimony oxide); 2968PHW8QP (Citric Acid); 9E7R5L6H31 (Oxalic Acid); 9IT35J3UV3 (Antimony); P217481X5E (antimony trioxide); W4888I119H (tartaric acid)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170630
[Lr] Data última revisão:
170630
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170603
[St] Status:MEDLINE


  5 / 1948 MEDLINE  
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[PMID]:28424520
[Au] Autor:Edwards JT; Merchant RR; McClymont KS; Knouse KW; Qin T; Malins LR; Vokits B; Shaw SA; Bao DH; Wei FL; Zhou T; Eastgate MD; Baran PS
[Ad] Endereço:Department of Chemistry, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA.
[Ti] Título:Decarboxylative alkenylation.
[So] Source:Nature;545(7653):213-218, 2017 05 11.
[Is] ISSN:1476-4687
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Olefin chemistry, through pericyclic reactions, polymerizations, oxidations, or reductions, has an essential role in the manipulation of organic matter. Despite its importance, olefin synthesis still relies largely on chemistry introduced more than three decades ago, with metathesis being the most recent addition. Here we describe a simple method of accessing olefins with any substitution pattern or geometry from one of the most ubiquitous and variegated building blocks of chemistry: alkyl carboxylic acids. The activating principles used in amide-bond synthesis can therefore be used, with nickel- or iron-based catalysis, to extract carbon dioxide from a carboxylic acid and economically replace it with an organozinc-derived olefin on a molar scale. We prepare more than 60 olefins across a range of substrate classes, and the ability to simplify retrosynthetic analysis is exemplified with the preparation of 16 different natural products across 10 different families.
[Mh] Termos MeSH primário: Alcenos/química
Alcenos/síntese química
Produtos Biológicos/química
Produtos Biológicos/síntese química
Ácidos Carboxílicos/química
[Mh] Termos MeSH secundário: Alcenos/classificação
Amidas/química
Produtos Biológicos/classificação
Dióxido de Carbono/química
Dióxido de Carbono/isolamento & purificação
Catálise
Ferro/química
Níquel/química
Oxirredução
Policetídeos/síntese química
Policetídeos/química
Especificidade por Substrato
Tartaratos/síntese química
Tartaratos/química
Zinco/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Alkenes); 0 (Amides); 0 (Biological Products); 0 (Carboxylic Acids); 0 (Polyketides); 0 (Tartrates); 142M471B3J (Carbon Dioxide); 7OV03QG267 (Nickel); E1UOL152H7 (Iron); J41CSQ7QDS (Zinc); OQ72CPY58Z (diethyl tartrate)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171019
[Lr] Data última revisão:
171019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170421
[St] Status:MEDLINE
[do] DOI:10.1038/nature22307


  6 / 1948 MEDLINE  
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[PMID]:28412486
[Au] Autor:Jiang J; Yang M; Gao Y; Wang J; Li D; Li T
[Ad] Endereço:School of Environment, Tsinghua University, Beijing, 100084, China; Key Laboratory of Solid Waste Management and Environment Safety (Tsinghua University), Ministry of Education, Tsinghua University, Beijing, 100084, China; Collaborative Innovation Center for Regional Environmental Quality, Tsinghua
[Ti] Título:Removal of toxic metals from vanadium-contaminated soils using a washing method: Reagent selection and parameter optimization.
[So] Source:Chemosphere;180:295-301, 2017 Aug.
[Is] ISSN:1879-1298
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Vanadium (V) contamination in soils is an increasing worldwide concern facing human health and environmental conservation. The fractionation of a metal influences its mobility and biological toxicity. We analyzed the fractionations of V and several other metals using the BCR three-step sequential extraction procedure. Among methods for removing metal contamination, soil washing is an effective permanent treatment. We conducted experiments to select the proper reagents and to optimize extraction conditions. Citric acid, tartaric acid, oxalic acid, and Na EDTA all exhibited high removal rates of the extractable state of V. With a liquid-to-solid ratio of 10, washing with 0.4 mol/L citric acid, 0.4 mol/L tartaric acid, 0.4 mol/L oxalic acid, and 0.12 mol/L Na EDTA led to removal rates of 91%, 88%, 88%, and 61%, respectively. The effect of multiple washing on removal rate was also explored. According to the changes observed in metal fractionations, differences in removal rates among reagents is likely associated with their pK value, pH in solution, and chemical structure. We concluded that treating with appropriate washing reagents under optimal conditions can greatly enhance the remediation of vanadium-contaminated soils.
[Mh] Termos MeSH primário: Recuperação e Remediação Ambiental/métodos
Poluentes do Solo/análise
Vanádio/análise
[Mh] Termos MeSH secundário: Fracionamento Químico
Ácido Cítrico
Poluição Ambiental
Seres Humanos
Indicadores e Reagentes
Oxalatos/química
Solo/química
Tartaratos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Indicators and Reagents); 0 (Oxalates); 0 (Soil); 0 (Soil Pollutants); 0 (Tartrates); 00J9J9XKDE (Vanadium); 2968PHW8QP (Citric Acid); W4888I119H (tartaric acid)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170417
[St] Status:MEDLINE


  7 / 1948 MEDLINE  
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[PMID]:28222860
[Au] Autor:Lai S; Tang S; Xie J; Cai C; Chen X; Chen C
[Ad] Endereço:Key Laboratory of Environmentally Friendly Chemistry and Applications of Ministry of Education, College of Chemistry, Xiangtan University, Xiangtan, Hunan 411105, China; School of Chemical Engineering, Xiangtan University, Xiangtan, Hunan 411105, China.
[Ti] Título:Highly efficient chiral separation of amlodipine enantiomers via triple recognition hollow fiber membrane extraction.
[So] Source:J Chromatogr A;1490:63-73, 2017 Mar 24.
[Is] ISSN:1873-3778
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:In this study, a triple recognition chiral extraction process has been developed to separate (S)-amlodipine from racemic raw medicine, based on the combination of molecularly imprinted hollow fiber membrane and cross-flow biphasic recognition extraction. The chiral separation process was operated in a dismountable hollow fiber module coated with molecularly imprinted polymer, and the cross-flow extraction was applied with d-tartaric acid in feed phase and sulfobutyl ether-ß-cyclodextrin in stripping phase. The synergistic effect of molecularly imprinted polymer and dual chiral additives was investigated, and excellent enantioseparation ability with a selectivity factor of 1.98 was obtained. Mathematical model of S/R=0.598e for racemic amlodipine separation by molecularly imprinted hollow fiber membrane extraction was established. The optical purity for amlodipine is up to 90% when 4 hollow fiber membrane modules of 25cm in length in series are used. The triple recognition process strongly enhanced the separation selectivity; therefore it may bring about the potentially application for large-scale production of pure enantiomeric compound.
[Mh] Termos MeSH primário: Anlodipino/isolamento & purificação
Fracionamento Químico/métodos
[Mh] Termos MeSH secundário: Anlodipino/química
Impressão Molecular
Estereoisomerismo
Tartaratos
beta-Ciclodextrinas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Tartrates); 0 (beta-Cyclodextrins); 1J444QC288 (Amlodipine); 2PP9364507 (SBE4-beta-cyclodextrin); W4888I119H (tartaric acid)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170223
[St] Status:MEDLINE


  8 / 1948 MEDLINE  
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[PMID]:28118562
[Au] Autor:Dalmia A
[Ad] Endereço:Perkin Elmer, 710 Bridgeport Ave, Shelton, CT 06484.
[Ti] Título:Rapid Measurement of Food Adulteration with Minimal Sample Preparation and No Chromatography Using Ambient Ionization Mass Spectrometry.
[So] Source:J AOAC Int;100(2):573-575, 2017 Mar 01.
[Is] ISSN:1060-3271
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A rapid method, with minimal sample preparation and no chromatography, was developed for analyzing food samples such as olive oil and pomegranate juice to measure adulteration with cheaper ingredients using the novel Direct Sample Analysis™ (DSA) ion source in conjunction with a time-of-flight (TOF)-MS. In less than 30 s, with minimal sample preparation and method development, adulteration of olive oil and pomegranate juice with cheaper seed oils and fruit juices, respectively, was measured with DSA/TOF-MS.
[Mh] Termos MeSH primário: Contaminação de Alimentos/análise
Sucos de Frutas e Vegetais/análise
Azeite de Oliva/análise
[Mh] Termos MeSH secundário: Malatos/análise
Espectrometria de Massas
Olea
Punicaceae
Óleo de Soja/análise
Tartaratos/análise
Triglicerídeos/análise
Trioleína/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (1,2-dioleoyl-3-palmitoylglycerol); 0 (1,3-dilinolenoyl-2-palmitoylglycerol); 0 (Malates); 0 (Olive Oil); 0 (Tartrates); 0 (Triglycerides); 122-32-7 (Triolein); 8001-22-7 (Soybean Oil); 817L1N4CKP (malic acid); V5LJ52OGS7 (trilinolein); W4888I119H (tartaric acid)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170606
[Lr] Data última revisão:
170606
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170125
[St] Status:MEDLINE
[do] DOI:10.5740/jaoacint.16-0343


  9 / 1948 MEDLINE  
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[PMID]:28100202
[Au] Autor:Fujiwara K; Kawamoto K; Shimizu Y; Fukuhara T; Koyama S; Kataoka H; Kitano H; Takeuchi H
[Ad] Endereço:Department of Otolaryngology, Head and Neck Surgery, Faculty of Medicine, Tottori University, 36-1, Nishimachi, Yonago, 683-8504, Japan. kfujiwa@med.tottori-u.ac.jp.
[Ti] Título:A novel reflex cough testing device.
[So] Source:BMC Pulm Med;17(1):19, 2017 Jan 18.
[Is] ISSN:1471-2466
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The reflex cough test is useful for detecting silent aspiration, a risk factor for aspiration pneumonia. However, assessing the risk of aspiration pneumonia requires measuring not only the cough reflex but also cough strength. Currently, no reflex cough testing device is available that can directly measure reflex cough strength. We therefore developed a new testing device that can easily and simultaneously measure cough strength and the time until the cough reflex, and verified whether screening with this new instrument is feasible for evaluating the risk of aspiration pneumonia. METHODS: This device consists of a special pipe with a double lumen, a nebulizer, and an electronic spirometer. We used a solution of prescription-grade L-tartaric acid to initiate the cough reflex. The solution was inhaled through a mouthpiece as a microaerosol produced by an ultrasonic nebulizer. The peak cough flow (PCF) of the induced cough was measured with the spirometer. The 70 patients who participated in this study comprised 49 patients without a history of pneumonia (group A), 21 patients with a history of pneumonia (group B), and 10 healthy volunteers (control group). RESULTS: With the novel device, PCF and time until cough reflex could be measured without adverse effects. The PCF values were 118.3 ± 64.0 L/min, 47.7 ± 38.5 L/min, and 254.9 ± 83.8 L/min in group A, group B, and the control group, respectively. The PCF of group B was significantly lower than that of group A and the control group (p < 0.0001), while that of group B was significantly lower than that of the control group (p < 0.0001). The time until the cough reflex was 4.2 ± 5.9 s, 7.0 ± 7.0 s, and 1 s in group A, group B, and the control group, respectively. This duration was significantly longer for groups A and B than for the control group (A: p < 0.001, B: p < 0.001), but there was no significant difference between groups A and B (p = 0.0907). CONCLUSION: Our newly developed device can easily and simultaneously measure the time until the cough reflex and the strength of involuntary coughs for assessment of patients at risk of aspiration pneumonia.
[Mh] Termos MeSH primário: Testes de Provocação Brônquica/instrumentação
Tosse/etiologia
Pneumonia Aspirativa/diagnóstico
Reflexo
Tartaratos/administração & dosagem
[Mh] Termos MeSH secundário: Idoso
Idoso de 80 Anos ou mais
Estudos de Casos e Controles
Tosse/induzido quimicamente
Feminino
Fluxo Expiratório Forçado
Seres Humanos
Japão
Masculino
Meia-Idade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Tartrates); W4888I119H (tartaric acid)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171102
[Lr] Data última revisão:
171102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170120
[St] Status:MEDLINE
[do] DOI:10.1186/s12890-017-0365-y


  10 / 1948 MEDLINE  
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[PMID]:28086783
[Au] Autor:Narnoliya LK; Kaushal G; Singh SP; Sangwan RS
[Ad] Endereço:Center of Innovative and Applied Bioprocessing (A National Institute under the Department of Biotechnology, Govt. of India), S.A.S. Nagar, Mohali, Punjab, India.
[Ti] Título:De novo transcriptome analysis of rose-scented geranium provides insights into the metabolic specificity of terpene and tartaric acid biosynthesis.
[So] Source:BMC Genomics;18(1):74, 2017 01 13.
[Is] ISSN:1471-2164
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Rose-scented geranium (Pelargonium sp.) is a perennial herb that produces a high value essential oil of fragrant significance due to the characteristic compositional blend of rose-oxide and acyclic monoterpenoids in foliage. Recently, the plant has also been shown to produce tartaric acid in leaf tissues. Rose-scented geranium represents top-tier cash crop in terms of economic returns and significance of the plant and plant products. However, there has hardly been any study on its metabolism and functional genomics, nor any genomic expression dataset resource is available in public domain. Therefore, to begin the gains in molecular understanding of specialized metabolic pathways of the plant, de novo sequencing of rose-scented geranium leaf transcriptome, transcript assembly, annotation, expression profiling as well as their validation were carried out. RESULTS: De novo transcriptome analysis resulted a total of 78,943 unique contigs (average length: 623 bp, and N50 length: 752 bp) from 15.44 million high quality raw reads. In silico functional annotation led to the identification of several putative genes representing terpene, ascorbic acid and tartaric acid biosynthetic pathways, hormone metabolism, and transcription factors. Additionally, a total of 6,040 simple sequence repeat (SSR) motifs were identified in 6.8% of the expressed transcripts. The highest frequency of SSR was of tri-nucleotides (50%). Further, transcriptome assembly was validated for randomly selected putative genes by standard PCR-based approach. In silico expression profile of assembled contigs were validated by real-time PCR analysis of selected transcripts. CONCLUSION: Being the first report on transcriptome analysis of rose-scented geranium the data sets and the leads and directions reflected in this investigation will serve as a foundation for pursuing and understanding molecular aspects of its biology, and specialized metabolic pathways, metabolic engineering, genetic diversity as well as molecular breeding.
[Mh] Termos MeSH primário: Perfilação da Expressão Gênica
Geranium/genética
Geranium/metabolismo
Tartaratos/metabolismo
Terpenos/metabolismo
Transcriptoma
[Mh] Termos MeSH secundário: Análise por Conglomerados
Biologia Computacional/métodos
Ontologia Genética
Sequenciamento de Nucleotídeos em Larga Escala
Redes e Vias Metabólicas
Repetições de Microssatélites
Anotação de Sequência Molecular
Fenótipo
Reprodutibilidade dos Testes
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Tartrates); 0 (Terpenes); W4888I119H (tartaric acid)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171120
[Lr] Data última revisão:
171120
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170115
[St] Status:MEDLINE
[do] DOI:10.1186/s12864-016-3437-0



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