Base de dados : MEDLINE
Pesquisa : D02.241.081.583 [Categoria DeCS]
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  1 / 1991 MEDLINE  
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[PMID]:28823875
[Au] Autor:Fakhari MA; Rahimpour F; Taran M
[Ad] Endereço:Biotechnology Research Lab., Faculty of Petroleum and Chemical Engineering, Razi University, Kermanshah 67149-67346, Iran.
[Ti] Título:Response surface methodology optimization of partitioning of xylanase form Aspergillus Niger by metal affinity polymer-salt aqueous two-phase systems.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;1063:1-10, 2017 Sep 15.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Aqueous two phase affinity partitioning system using metal ligands was applied for partitioning and purification of xylanase produced by Aspergillus Niger. To minimization the number of experiments for the design parameters and develop predictive models for optimization of the purification process, response surface methodology (RSM) with a face-centered central composite design (CCF) has been used. Polyethylene glycol (PEG) 6000 was activated using epichlorohydrin, covalently linked to iminodiacetic acid (IDA), and the specific metal ligand Cu was attached to the polyethylene glycol-iminodiacetic acid (PEG-IDA). The influence of some experimental variables such as PEG (10-18%w/w), sodium sulfate (8-12%), PEG-IDA-Cu concentration (0-50% w/w of total PEG), pH of system (4-8) and crude enzyme loading (6-18%w/w) on xylanase and total protein partitioning coefficient, enzyme yield and enzyme specific activity were systematically evaluated. Two optimal point with high enzyme partitioning factor 10.97 and yield 79.95 (including 10% PEG, 12% Na SO , 50% ligand, pH 8 and 6% crude enzyme loading) and high specific activity in top phase 42.21 (including 14.73% PEG, 8.02% Na SO , 28.43% ligand, pH 7.7 and 6.08% crude enzyme loading) were attained. The adequacy of the RSM models was verified by a good agreement between experimental and predicted results.
[Mh] Termos MeSH primário: Aspergillus niger/enzimologia
Fracionamento Químico/métodos
Endo-1,4-beta-Xilanases/isolamento & purificação
Proteínas Fúngicas/isolamento & purificação
[Mh] Termos MeSH secundário: Aspergillus niger/química
Cobre/química
Endo-1,4-beta-Xilanases/análise
Endo-1,4-beta-Xilanases/química
Proteínas Fúngicas/análise
Proteínas Fúngicas/química
Concentração de Íons de Hidrogênio
Iminoácidos/química
Polietilenoglicóis/química
Sulfatos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fungal Proteins); 0 (Imino Acids); 0 (Sulfates); 0 (poly(ethylene glycol-iminodiacetic acid)); 0YPR65R21J (sodium sulfate); 30IQX730WE (Polyethylene Glycols); 789U1901C5 (Copper); EC 3.2.1.8 (Endo-1,4-beta Xylanases)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171002
[Lr] Data última revisão:
171002
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170822
[St] Status:MEDLINE


  2 / 1991 MEDLINE  
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[PMID]:28566650
[Au] Autor:Inokuma T; Jichu T; Nishida K; Shigenaga A; Otaka A
[Ad] Endereço:Institute of Biomedical Sciences and Graduate School of Pharmaceutical Sciences, Tokushima University.
[Ti] Título:A Convenient Method for Preparation of α-Imino Carboxylic Acid Derivatives and Application to the Asymmetric Synthesis of Unnatural α-Amino Acid Derivative.
[So] Source:Chem Pharm Bull (Tokyo);65(6):573-581, 2017.
[Is] ISSN:1347-5223
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:We describe herein a manganese(IV) oxide-mediated oxidation of N-p-methoxyphenyl (PMP)-protected glycine derivatives for the synthesis of α-imino carboxylic acid derivatives. Using this methodology, utilization of unstable glyoxic acid derivatives was avoided. Furthermore, using this methodology we synthesized novel α-imino carboxylic acid derivatives such as α-imino phenyl ester, perfluoroalkyl etsers, imides, and thioester. The asymmetric Mannich reaction of those novel imine derivatives with 1,3-dicarbonyl compound is also described, and the novel α-imino imide gave improved chemical yield and stereoselectivity compared with those obtained by the use of the conventional α-imino ester-type substrate.
[Mh] Termos MeSH primário: Aminoácidos/síntese química
Ácidos Carboxílicos/química
Iminoácidos/química
[Mh] Termos MeSH secundário: Espectroscopia de Ressonância Magnética Nuclear de Carbono-13
Espectroscopia de Prótons por Ressonância Magnética
Espectrometria de Massas por Ionização por Electrospray
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amino Acids); 0 (Carboxylic Acids); 0 (Imino Acids)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170705
[Lr] Data última revisão:
170705
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170602
[St] Status:MEDLINE
[do] DOI:10.1248/cpb.c17-00158


  3 / 1991 MEDLINE  
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[PMID]:28486765
[Au] Autor:Tomita T; Yin L; Nakamura S; Kosono S; Kuzuyama T; Nishiyama M
[Ad] Endereço:Biotechnology Research Center, The University of Tokyo, Japan.
[Ti] Título:Crystal structure of the 2-iminoglutarate-bound complex of glutamate dehydrogenase from Corynebacterium glutamicum.
[So] Source:FEBS Lett;591(11):1611-1622, 2017 Jun.
[Is] ISSN:1873-3468
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The NADP -dependent glutamate dehydrogenase from Corynebacterium glutamicum (CgGDH) is considered to be one of the key enzymes in the industrial fermentation of glutamate due to its high glutamate-producing activity. We determined the crystal structure of CgGDH complexed with NADP and 2-iminoglutarate. Among six subunits of hexameric CgGDH-binding NADP , only four subunits bind 2-iminoglutarate in a closed form, while the other two are in an open form. In the closed form, 2-iminoglutarate is bound to the substrate-binding site with the 2-imino group stacked by the nicotinamide ring of the coenzyme, suggesting a prehydride transfer state in a hypothesized reaction scheme with the imino intermediate. We also conducted MD simulations and provide insights into the extreme preference for the glutamate-producing reaction of CgGDH. DATABASE: The atomic coordinate and structure factors have been deposited in the RCSB PDB database under the accession number 5GUD.
[Mh] Termos MeSH primário: Corynebacterium glutamicum/enzimologia
Glutamato Desidrogenase/química
[Mh] Termos MeSH secundário: Glutamato Desidrogenase/metabolismo
Glutaratos/metabolismo
Iminoácidos/metabolismo
Simulação de Dinâmica Molecular
NADP/metabolismo
[Pt] Tipo de publicação:LETTER
[Nm] Nome de substância:
0 (Glutarates); 0 (Imino Acids); 23648-80-8 (iminoglutarate); 53-59-8 (NADP); EC 1.4.1.2 (Glutamate Dehydrogenase)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170915
[Lr] Data última revisão:
170915
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170510
[St] Status:MEDLINE
[do] DOI:10.1002/1873-3468.12667


  4 / 1991 MEDLINE  
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[PMID]:28477870
[Au] Autor:Piron L; Deshayes E; Escal L; Souche R; Herrero A; Pierredon-Foulongne MA; Assenat E; le Lam N; Quenet F; Guiu B
[Ad] Endereço:Saint-Éloi University Hospital, Department of Radiology, 80, avenue Augustin-Fliche, 34090 Montpellier, France. Electronic address: l-piron@chu-montpellier.fr.
[Ti] Título:[Portal vein embolization: Present and future].
[Ti] Título:Embolisation portale préopératoire : présent et futur..
[So] Source:Bull Cancer;104(5):407-416, 2017 May.
[Is] ISSN:1769-6917
[Cp] País de publicação:France
[La] Idioma:fre
[Ab] Resumo:Portal vein embolization consists of occluding a part of the portal venous system in order to achieve the hypertrophy of the non-embolized liver segments. This technique is used during the preoperative period of major liver resection when the future remnant liver (FRL) volume is insufficient, exposing to postoperative liver failure, main cause of death after major hepatectomy. Portal vein embolization indication depends on the FRL, commonly assessed by its volume. Nowadays, FRL function evaluation seems more relevant and can be measured by 99mTc labelled mebrofenin scintigraphy. Portal vein embolization procedure is mostly performed with percutaneous trans-hepatic access by using ultrasonography guidance and consists of embolic agent injection, such as cyanoacrylate, in the targeted portal vein branches with fluoroscopic guidance. It is a safe and well-tolerated technique, with extremely low morbi-mortality. Portal vein embolization leads to sufficient FRL hypertrophy in about 80% of patients, allowing them to undergo surgery from which they were initially rejected. The two main reasons of non-resection are tumor progression (≈15% of cases) and FRL insufficient hypertrophy (≈5% of cases). When portal vein embolization is not enough to obtain adequate FRL regeneration, hepatic vein embolization may potentiate its effect (liver venous deprivation technique).
[Mh] Termos MeSH primário: Embolização Terapêutica/métodos
Neoplasias Hepáticas/terapia
Veia Porta
Cuidados Pré-Operatórios
[Mh] Termos MeSH secundário: Quimioterapia Adjuvante
Cianoacrilatos/administração & dosagem
Embolização Terapêutica/efeitos adversos
Embolização Terapêutica/mortalidade
Hepatectomia/efeitos adversos
Hepatectomia/métodos
Hepatectomia/mortalidade
Seres Humanos
Hipertrofia/etiologia
Iminoácidos
Fígado/irrigação sanguínea
Fígado/diagnóstico por imagem
Fígado/patologia
Falência Hepática/mortalidade
Falência Hepática/prevenção & controle
Neoplasias Hepáticas/irrigação sanguínea
Neoplasias Hepáticas/diagnóstico por imagem
Regeneração Hepática
Meia-Idade
Compostos de Organotecnécio
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Cyanoacrylates); 0 (Imino Acids); 0 (Organotechnetium Compounds); F2NQ468L52 (technetium Tc 99m mebrofenin)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170809
[Lr] Data última revisão:
170809
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170508
[St] Status:MEDLINE


  5 / 1991 MEDLINE  
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[PMID]:28381385
[Au] Autor:Goussous N; Maqsood H; Spiegler E; Kowdley GC; Cunningham SC
[Ad] Endereço:Department of Surgery, Saint Agnes Hospital, Baltimore, USA. Steven.Cunningham@stagnes.org.
[Ti] Título:HIDA scan for functional gallbladder disorder: ensure that you know how the scan was done.
[So] Source:Hepatobiliary Pancreat Dis Int;16(2):197-201, 2017 Apr.
[Is] ISSN:1499-3872
[Cp] País de publicação:Singapore
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Despite the increasing use of fatty meal (FM) as a substitute for cholecystokinin (CCK) in pain reproduction during hepato-imino-diacetic acid (HIDA) scan in functional gallbladder disorder, there are no studies comparing the differences between CCK and FM. The present study was to compare the efficacy of FM in comparison of CCK in FGBD application. METHODS: Patients undergoing HIDA scans from August 2013 to May 2014 were divided into two groups: those undergoing CCK-stimulated HIDA scan versus FM-stimulated HIDA scan. These groups were compared according to demographics and HIDA results. RESULTS: Of 153 patients, 70 received CCK and 83 FM. There was no difference regarding age, gender, gallstones, gallbladder ejection fraction and time to visualization. However, significantly more of the patients receiving CCK than FM experienced pain reproduction (61% vs 30%, P<0.01). CONCLUSIONS: Stimulation of gallbladder contractility with a FM during HIDA is less than half as likely to reproduce biliary symptoms compared to CCK, despite similar ejection fractions and other parameters. It is essential that providers account for this difference when counseling patients regarding cholecystectomy for functional gallbladder disorder.
[Mh] Termos MeSH primário: Discinesia Biliar/diagnóstico por imagem
Vesícula Biliar/diagnóstico por imagem
Iminoácidos/administração & dosagem
Compostos Radiofarmacêuticos/administração & dosagem
[Mh] Termos MeSH secundário: Dor Abdominal/etiologia
Adolescente
Adulto
Idoso
Idoso de 80 Anos ou mais
Discinesia Biliar/fisiopatologia
Discinesia Biliar/cirurgia
Colecistectomia
Colecistocinina/administração & dosagem
Colecistocinina/efeitos adversos
Gorduras na Dieta/administração & dosagem
Gorduras na Dieta/efeitos adversos
Feminino
Vesícula Biliar/fisiopatologia
Vesícula Biliar/cirurgia
Seres Humanos
Masculino
Meia-Idade
Valor Preditivo dos Testes
Vitamina K/administração & dosagem
Vitamina K/efeitos adversos
Adulto Jovem
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Dietary Fats); 0 (Ensure Plus); 0 (Imino Acids); 0 (Radiopharmaceuticals); 12001-79-5 (Vitamin K); 9011-97-6 (Cholecystokinin); EK22QV7701 (lidofenin)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171023
[Lr] Data última revisão:
171023
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170407
[St] Status:MEDLINE


  6 / 1991 MEDLINE  
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[PMID]:28369856
[Au] Autor:Yue JY; Wang JZ; Zhang CH; Jia W; Li X; Sun Z
[Ad] Endereço:Inst. of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
[Ti] Título:Effects of Hot-Pressure Extraction Time on Composition and Gelatin Properties of Chicken Bone Extracts.
[So] Source:J Food Sci;82(5):1066-1075, 2017 May.
[Is] ISSN:1750-3841
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Hot-pressure extraction was utilized in this study to extract proteins from chicken bones at 130 °C. The obtained extracts were further used to prepare gelatin gels. Results demonstrated that the extraction time can significantly affect the composition of the chicken bone extracts (P < 0.05). High-performance liquid chromatography (HPLC) analysis indicated that the protein fraction of molecular weight (MW) >30 KDa was only visible in the extracts collected between 40 and 60 min. The highest contents of hydroxyproline, imino acids, and hydrophobic amino acids were all achieved in the chicken bone extracts after 120 min of extraction, being 3.9, 7.7, and 16.0 mg/g, respectively. The prepared gelatin properties were evaluated in terms of viscosity, storage and loss modulus, stability, gel strength, and their microstructures. Results indicated that gelatins made from chicken bone extracts of 20, 40, and 60 min extraction had better properties compared to that of 90 and 120 min. Significant correlations were identified between gelatin's composition and properties (P < 0.05). The abundance of proteins with MW of <10 KDa and 10 to 30 KDa was found to be the predominant factor that can affect the gelatin's properties. This study illustrated a promising and natural way to obtain edible gelatins from chicken bones.
[Mh] Termos MeSH primário: Aminoácidos/análise
Osso e Ossos/química
Proteínas na Dieta/análise
Manipulação de Alimentos
Gelatina/análise
Temperatura Alta
Pressão
[Mh] Termos MeSH secundário: Animais
Galinhas
Cromatografia Líquida de Alta Pressão/métodos
Gelatina/isolamento & purificação
Géis/química
Seres Humanos
Hidroxiprolina/análise
Iminoácidos/análise
Peso Molecular
Viscosidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amino Acids); 0 (Dietary Proteins); 0 (Gels); 0 (Imino Acids); 9000-70-8 (Gelatin); RMB44WO89X (Hydroxyproline)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171102
[Lr] Data última revisão:
171102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170404
[St] Status:MEDLINE
[do] DOI:10.1111/1750-3841.13687


  7 / 1991 MEDLINE  
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[PMID]:28343106
[Au] Autor:Karkra K; Tetala KKR; Vijayalakshmi MA
[Ad] Endereço:Advanced Centre for Bioseparation Technology (CBST), VIT University, Vellore, Tamilnadu 632014, India.
[Ti] Título:A structure based plasma protein pre-fractionation using conjoint immobilized metal/chelate affinity (IMA) system.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;1052:1-9, 2017 May 01.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The potential of immobilized metal/chelate affinity (IMA) in a continuous fashion, referred as conjoint approach, to pre-fractionate plasma proteins (in their native state) prior to LC-MS analysis was investigated in this study. Four transition metal-ions (Co (II), Zn (II), Ni (II) and Cu (II)) were individually chelated with IDA (iminodiacetic acid) coated CIM (Convective Interaction Media) disks and placed in a single housing in the following sequential order: IDA-Co (II)→IDA-Zn (II)→IDA-Ni (II)→IDA-Cu (II). The rationale behind this order is to retain proteins based on their specific requirement for surface exposed histidine topography. This structural pre-fractionation hypothesis was successfully proven using four human plasma proteins (fibrinogen, IgG, transferrin, and albumin) with varying histidine topographies. This conjoint IMA pre-fractionation strategy not only fractionated proteins (from plasma) based on their native surface histidine topography, but also identified 157 proteins from human plasma. The advantage of our conjoint IMA is its ability to fractionate proteins in their native state and reduce plasma complexity in a single step by employing single buffer system.
[Mh] Termos MeSH primário: Proteínas Sanguíneas/isolamento & purificação
Quelantes/química
Fracionamento Químico/métodos
Histidina/isolamento & purificação
Iminoácidos/química
Metais/química
[Mh] Termos MeSH secundário: Adsorção
Proteínas Sanguíneas/análise
Cromatografia Líquida/métodos
Histidina/análise
Seres Humanos
Proteômica/métodos
Espectrometria de Massas em Tandem/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Blood Proteins); 0 (Chelating Agents); 0 (Imino Acids); 0 (Metals); 4QD397987E (Histidine); XQM2L81M8Z (iminodiacetic acid)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170526
[Lr] Data última revisão:
170526
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170327
[St] Status:MEDLINE


  8 / 1991 MEDLINE  
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[PMID]:28256211
[Au] Autor:Alioto A; Di Lorenzo C; Montgomery ML; Yacob D
[Ad] Endereço:Section of Pediatric Psychology and Neuropsychology, Nationwide Children's Hospital, Department of Pediatrics, The Ohio State University, Columbus, OH. Electronic address: anthony.alioto@nationwidechildrens.org.
[Ti] Título:High Cost and Low Yield: The Diagnostic Evaluation of Rumination Syndrome in Pediatrics.
[So] Source:J Pediatr;185:155-159, 2017 Jun.
[Is] ISSN:1097-6833
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: To document the use of diagnostic testing in adolescents who ultimately were diagnosed with rumination syndrome, a functional gastrointestinal disorder. We examined the diagnostic yield of each test as well as the associated costs, and we determined if any demographic or illness-related variables impacted the magnitude of the work-up. STUDY DESIGN: A retrospective chart review was conducted for 68 patients with rumination syndrome admitted to our inpatient treatment program. The cost and findings of patients' diagnostic investigations were gathered, as well as demographic and illness-related variables to determine factors that may be related to evaluation size. RESULTS: The most commonly used tests in the evaluation of rumination syndrome included esophagogastroduodenoscopy, gastric emptying, antroduodenal manometry, upper gastrointestinal series, and abdominal ultrasound scan. Each patient underwent an average of 8.8 tests, with the average cost for each patient's diagnostic work-up being US $19 795. Few tests were found to be beneficial in the diagnosis of rumination syndrome, and few demographic or illness variables were found to be related to the overall extent of the investigation. CONCLUSIONS: Extensive testing for rumination syndrome in adolescents is common in clinical practice, and comes at a high financial cost with low yield, likely delaying diagnosis and treatment. Symptom-based criteria should be used to make the diagnosis of rumination syndrome.
[Mh] Termos MeSH primário: Transtornos de Alimentação na Infância/diagnóstico
Transtornos de Alimentação na Infância/economia
[Mh] Termos MeSH secundário: Dor Abdominal/etiologia
Adolescente
Diagnóstico por Imagem/economia
Impedância Elétrica
Endoscopia Gastrointestinal/economia
Feminino
Esvaziamento Gástrico
Seres Humanos
Iminoácidos/economia
Masculino
Manometria/economia
Ohio
Qualidade de Vida
Estudos Retrospectivos
Inquéritos e Questionários
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Imino Acids); XQM2L81M8Z (iminodiacetic acid)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170304
[St] Status:MEDLINE


  9 / 1991 MEDLINE  
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[PMID]:28153357
[Au] Autor:Pes L; Kim Y; Tung CH
[Ad] Endereço:Molecular Imaging Innovations Institute, Department of Radiology, Weill Cornell Medicine, New York, NY 10021, USA.
[Ti] Título:Bidentate iminodiacetate modified dendrimer for bone imaging.
[So] Source:Bioorg Med Chem Lett;27(5):1252-1255, 2017 Mar 01.
[Is] ISSN:1464-3405
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A new dendrimer probe was designed for bone imaging. Bidentate iminodiacetate groups were introduced to the probe to obtain strong bind to bones. The assembled dendrimeric probe, with four iminodiacetate moieties and a fluorescent tag, displayed good selectivity to hydroxyapatite, calcium oxalate and calcium phosphate salts. In mice, the probe offered vivid skeletal details after intravenous delivery.
[Mh] Termos MeSH primário: Osso e Ossos
Dendrímeros/química
Diagnóstico por Imagem
Corantes Fluorescentes/química
Iminoácidos/química
Polilisina/síntese química
[Mh] Termos MeSH secundário: Administração Intravenosa
Animais
Osso e Ossos/diagnóstico por imagem
Camundongos
Estrutura Molecular
Polilisina/química
Polilisina/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Dendrimers); 0 (Fluorescent Dyes); 0 (Imino Acids); 25104-18-1 (Polylysine)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171017
[Lr] Data última revisão:
171017
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170204
[St] Status:MEDLINE


  10 / 1991 MEDLINE  
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[PMID]:27750088
[Au] Autor:Zhang Q; Zhao H; Li D; Liu L; Du S
[Ad] Endereço:School of Pharmacy, Nanjing Medical University, Nanjing 211166, China. Electronic address: zhtsing101@sina.com.
[Ti] Título:A surface-grafted ligand functionalization strategy for coordinate binding of doxorubicin at surface of PEGylated mesoporous silica nanoparticles: Toward pH-responsive drug delivery.
[So] Source:Colloids Surf B Biointerfaces;149:138-145, 2017 Jan 01.
[Is] ISSN:1873-4367
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:To achieve drug targeting and on-demand releasing, surface functionalization plays a critical role in fabricating potential mesoporous silica nanoparticles (MSNs) toward tumor chemotherapy. Here, we prepared a size-controllable ligand-functionalized MSNs delivery system via coordinate bonding, which can release doxorubicin (DOX) in response to pH and prolong the circulation time of drug in vivo. After modifying the external surface of MSNs with polyethylene glycol (PEG), iminodiacetic acid (IDA) as a ligand was mainly grafted on the surface of mesopores to chelate cupric iron and DOX in sequence via coordinate bonds. The modified MSNs exhibited a uniform size of about 72nm and could be stably dispersed in saline. After DOX loading, the drug loading content and encapsulation efficiency were calculated to be 9.3±0.1% and 92.8±0.6%, respectively. Moreover, the resultant MSNs showed a pH-responsive release property, which could avoid the premature leakage of drug in circulation and achieve on-demand release within the tumor cells. Additionally, the pharmacokinetic study in healthy rats demonstrated that DOX loaded in functionalized MSNs presented the longer circulation time and lower plasma clearance rate compared with DOX solution. These results indicated that PEG/IDA modified MSNs with pH-responsive release capacity possessed great promising as an anticancer drug delivery system.
[Mh] Termos MeSH primário: Antibióticos Antineoplásicos/farmacocinética
Doxorrubicina/farmacocinética
Portadores de Fármacos
Nanopartículas/química
Polietilenoglicóis/química
Dióxido de Silício/química
[Mh] Termos MeSH secundário: Animais
Antibióticos Antineoplásicos/sangue
Antibióticos Antineoplásicos/química
Doxorrubicina/sangue
Doxorrubicina/química
Composição de Medicamentos
Liberação Controlada de Fármacos
Concentração de Íons de Hidrogênio
Iminoácidos/química
Masculino
Nanopartículas/ultraestrutura
Tamanho da Partícula
Porosidade
Ratos
Ratos Sprague-Dawley
Propriedades de Superfície
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibiotics, Antineoplastic); 0 (Drug Carriers); 0 (Imino Acids); 30IQX730WE (Polyethylene Glycols); 7631-86-9 (Silicon Dioxide); 80168379AG (Doxorubicin); XQM2L81M8Z (iminodiacetic acid)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170224
[Lr] Data última revisão:
170224
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161018
[St] Status:MEDLINE



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