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  1 / 1642 MEDLINE  
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[PMID]:28459528
[Au] Autor:Laverty DJ; Averill AM; Doublié S; Greenberg MM
[Ad] Endereço:Department of Chemistry, Johns Hopkins University , 3400 N. Charles St., Baltimore, Maryland 21218, United States.
[Ti] Título:The A-Rule and Deletion Formation During Abasic and Oxidized Abasic Site Bypass by DNA Polymerase θ.
[So] Source:ACS Chem Biol;12(6):1584-1592, 2017 06 16.
[Is] ISSN:1554-8937
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:DNA polymerase θ (Pol θ) is implicated in various cellular processes including double-strand break repair and apurinic/apyrimidinic site bypass. Because Pol θ expression correlates with poor cancer prognosis, the ability of Pol θ to bypass the C4'-oxidized abasic site (C4-AP) and 2-deoxyribonolactone (L), which are generated by cytotoxic agents, is of interest. Translesion synthesis and subsequent extension by Pol θ past C4-AP or L and an abasic site (AP) or its tetrahydrofuran analogue (F) was examined. Pol θ conducts translesion synthesis on templates containing AP and F with similar efficiencies and follows the "A-rule," inserting nucleotides in the order A > G > T. Translesion synthesis on templates containing C4-AP and L is less efficient than AP and F, and the preference for A insertion is reduced for L and absent for C4-AP. Extension past all abasic lesions (AP, F, C4-AP, and L) was significantly less efficient than translesion synthesis and yielded deletions caused by the base one or two nucleotides downstream from the lesion being used as a template, with the latter being favored. These results suggest that bypass of abasic lesions by Pol θ is highly mutagenic.
[Mh] Termos MeSH primário: DNA Polimerase beta/fisiologia
Mutagênese
[Mh] Termos MeSH secundário: Animais
Sequência de Bases
Dano ao DNA
DNA Polimerase beta/metabolismo
Reparo do DNA
Seres Humanos
Nucleotídeos/metabolismo
Oxirredução
Deleção de Sequência
Açúcares Ácidos
Moldes Genéticos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Nucleotides); 0 (Sugar Acids); 34371-14-7 (2,4,5-trihydroxypentanoic acid gamma-lactone); EC 2.7.7.- (DNA Polymerase beta)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:180201
[Lr] Data última revisão:
180201
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170502
[St] Status:MEDLINE
[do] DOI:10.1021/acschembio.7b00211


  2 / 1642 MEDLINE  
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[PMID]:28874446
[Au] Autor:Harper M; Wright A; St Michael F; Li J; Deveson Lucas D; Ford M; Adler B; Cox AD; Boyce JD
[Ad] Endereço:Infection and Immunity Program, Monash Biomedicine Discovery Institute and Department of Microbiology, Monash University, VIC, Australia marina.harper@monash.edu.
[Ti] Título:Characterization of Two Novel Lipopolysaccharide Phosphoethanolamine Transferases in Pasteurella multocida and Their Role in Resistance to Cathelicidin-2.
[So] Source:Infect Immun;85(11), 2017 Nov.
[Is] ISSN:1098-5522
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The lipopolysaccharide (LPS) produced by the Gram-negative bacterial pathogen has phosphoethanolamine (PEtn) residues attached to lipid A, 3-deoxy-d-manno-octulosonic acid (Kdo), heptose, and galactose. In this report, we show that PEtn is transferred to lipid A by the EptA homologue, PetL, and is transferred to galactose by a novel PEtn transferase that is unique to called PetG. Transcriptomic analyses indicated that expression was positively regulated by the global regulator Fis and negatively regulated by an Hfq-dependent small RNA. Importantly, we have identified a novel PEtn transferase called PetK that is responsible for PEtn addition to the single Kdo molecule (Kdo ), directly linked to lipid A in the glycoform A LPS. assays showed that the presence of a functional and , and therefore the presence of PEtn on lipid A and Kdo , was essential for resistance to the cationic, antimicrobial peptide cathelicidin-2. The importance of PEtn on Kdo and the identification of the transferase responsible for this addition have not previously been shown. Phylogenetic analysis revealed that PetK is the first representative of a new family of predicted PEtn transferases. The PetK family consists of uncharacterized proteins from a range of Gram-negative bacteria that produce LPS glycoforms with only one Kdo molecule, including pathogenic species within the genera , , and We predict that many of these bacteria will require the addition of PEtn to Kdo for maximum protection against host antimicrobial peptides.
[Mh] Termos MeSH primário: Proteínas de Bactérias/genética
Proteínas Sanguíneas/toxicidade
Farmacorresistência Bacteriana/genética
Etanolaminofosfotransferase/genética
Regulação Bacteriana da Expressão Gênica
Pasteurella multocida/genética
Pasteurella multocida/patogenicidade
Precursores de Proteínas/toxicidade
[Mh] Termos MeSH secundário: Animais
Proteínas de Bactérias/metabolismo
Galinhas
Biologia Computacional
Etanolaminofosfotransferase/metabolismo
Etanolaminas/química
Etanolaminas/metabolismo
Fator Proteico para Inversão de Estimulação/genética
Fator Proteico para Inversão de Estimulação/metabolismo
Galactose/química
Galactose/metabolismo
Perfilação da Expressão Gênica
Heptoses/química
Heptoses/metabolismo
Isoenzimas
Lipídeo A/química
Lipídeo A/metabolismo
Mutação
Proteínas Nucleares/genética
Proteínas Nucleares/metabolismo
Infecções por Pasteurella/microbiologia
Infecções por Pasteurella/patologia
Pasteurella multocida/classificação
Pasteurella multocida/efeitos dos fármacos
Filogenia
Açúcares Ácidos/química
Açúcares Ácidos/metabolismo
Transcriptoma
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Blood Proteins); 0 (Ethanolamines); 0 (Factor For Inversion Stimulation Protein); 0 (Heptoses); 0 (Isoenzymes); 0 (Lipid A); 0 (Nuclear Proteins); 0 (Protein Precursors); 0 (Sugar Acids); 0 (cathelicidin 2 protein, mammal); 1069-03-0 (2-keto-3-deoxyoctonate); 78A2BX7AEU (phosphorylethanolamine); EC 2.7.8.1 (Ethanolaminephosphotransferase); X2RN3Q8DNE (Galactose)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171024
[Lr] Data última revisão:
171024
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170907
[St] Status:MEDLINE


  3 / 1642 MEDLINE  
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[PMID]:28844838
[Au] Autor:Yao R; Hou W; Bao J
[Ad] Endereço:State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China.
[Ti] Título:Complete oxidative conversion of lignocellulose derived non-glucose sugars to sugar acids by Gluconobacter oxydans.
[So] Source:Bioresour Technol;244(Pt 1):1188-1192, 2017 Nov.
[Is] ISSN:1873-2976
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Non-glucose sugars derived from lignocellulose cover approximately 40% of the total carbohydrates of lignocellulose biomass. The conversion of the non-glucose sugars to the target products is an important task of lignocellulose biorefining research. Here we report a fast and complete conversion of the total non-glucose sugars from corn stover into the corresponding sugar acids by whole cell catalysis and aerobic fermentation of Gluconobacter oxydans. The conversions include xylose to xylonate, arabinose to arabonate, mannose to mannonate, and galactose to galactonate, as well as with glucose into gluconate. These cellulosic non-glucose sugar acids showed the excellent cement retard setting property. The mixed cellulosic sugar acids could be used as cement retard additives without separation. The conversion of the non-glucose sugars not only makes full use of lignocellulose derived sugars, but also effectively reduces the wastewater treatment burden by removal of residual sugars.
[Mh] Termos MeSH primário: Gluconobacter oxydans
Lignina
[Mh] Termos MeSH secundário: Fermentação
Glucose
Açúcares Ácidos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Sugar Acids); 11132-73-3 (lignocellulose); 9005-53-2 (Lignin); IY9XDZ35W2 (Glucose)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171023
[Lr] Data última revisão:
171023
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170829
[St] Status:MEDLINE


  4 / 1642 MEDLINE  
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[PMID]:28682052
[Au] Autor:Wang W; Zhu Y; Chen X
[Ad] Endereço:College of Chemistry and Molecular Engineering, ‡Peking-Tsinghua Center for Life Sciences, §Synthetic and Functional Biomolecules Center, and ∥Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Peking University , Beijing 100871, China.
[Ti] Título:Selective Imaging of Gram-Negative and Gram-Positive Microbiotas in the Mouse Gut.
[So] Source:Biochemistry;56(30):3889-3893, 2017 Aug 01.
[Is] ISSN:1520-4995
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The diverse gut microbial communities are crucial for host health. How the interactions between microbial communities and between host and microbes influence the host, however, is not well understood. To facilitate gut microbiota research, selective imaging of specific groups of microbiotas in the gut is of great utility but remains technically challenging. Here we present a chemical approach that enables selective imaging of Gram-negative and Gram-positive microbiotas in the mouse gut by exploiting their distinctive cell wall components. Cell-selective labeling is achieved by the combined use of metabolic labeling of Gram-negative bacterial lipopolysaccharides with a clickable azidosugar and direct labeling of Gram-positive bacteria with a vancomycin-derivatized fluorescent probe. We demonstrated this strategy by two-color fluorescence imaging of Gram-negative and Gram-positive gut microbiotas in the mouse intestines. This chemical method should be broadly applicable to different gut microbiota research fields and other bacterial communities studied in microbiology.
[Mh] Termos MeSH primário: Técnicas de Diagnóstico do Sistema Digestório
Disbiose/diagnóstico por imagem
Microbioma Gastrointestinal
Trato Gastrointestinal/diagnóstico por imagem
Bactérias Gram-Negativas/isolamento & purificação
Bactérias Gram-Positivas/isolamento & purificação
[Mh] Termos MeSH secundário: Animais
Azidas/análise
Azidas/química
Azidas/metabolismo
Azidas/farmacologia
Carbocianinas/análise
Parede Celular/química
Química Click
Disbiose/microbiologia
Corantes Fluorescentes/análise
Corantes Fluorescentes/química
Corantes Fluorescentes/metabolismo
Corantes Fluorescentes/farmacologia
Trato Gastrointestinal/microbiologia
Bactérias Gram-Negativas/citologia
Bactérias Gram-Negativas/crescimento & desenvolvimento
Bactérias Gram-Negativas/metabolismo
Bactérias Gram-Positivas/citologia
Bactérias Gram-Positivas/crescimento & desenvolvimento
Bactérias Gram-Positivas/metabolismo
Lipopolissacarídeos/análise
Lipopolissacarídeos/biossíntese
Lipopolissacarídeos/química
Camundongos Endogâmicos C57BL
Viabilidade Microbiana/efeitos dos fármacos
Imagem Óptica
Projetos Piloto
Porfobilinogênio/análogos & derivados
Porfobilinogênio/análise
Porfobilinogênio/química
Rodaminas/análise
Rodaminas/química
Organismos Livres de Patógenos Específicos
Açúcares Ácidos/análise
Açúcares Ácidos/química
Açúcares Ácidos/metabolismo
Açúcares Ácidos/farmacologia
Vancomicina/análogos & derivados
Vancomicina/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE; VALIDATION STUDIES
[Nm] Nome de substância:
0 (3-anilino difluoroboron dipyrromethene); 0 (5-carboxytetramethylrhodamine succinimidyl ester); 0 (8-azido-3,8-dideoxyoctulosonate); 0 (Alexa Fluor 647); 0 (Azides); 0 (Carbocyanines); 0 (Fluorescent Dyes); 0 (Lipopolysaccharides); 0 (Rhodamines); 0 (Sugar Acids); 1069-03-0 (2-keto-3-deoxyoctonate); 6Q205EH1VU (Vancomycin); 74KHC72QXK (Porphobilinogen)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170811
[Lr] Data última revisão:
170811
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170707
[St] Status:MEDLINE
[do] DOI:10.1021/acs.biochem.7b00539


  5 / 1642 MEDLINE  
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[PMID]:28485215
[Au] Autor:Almqvist H; Sandahl M; Lidén G
[Ad] Endereço:a Department of Chemical Engineering , Lund University , Lund , Sweden.
[Ti] Título:A rapid method for analysis of fermentatively produced D-xylonate using ultra-high performance liquid chromatography and evaporative light scattering detection.
[So] Source:Biosci Biotechnol Biochem;81(6):1078-1080, 2017 Jun.
[Is] ISSN:1347-6947
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:An ultra-high performance liquid chromatography (UHPLC) based method for the analysis of d-xylonate was developed using an amide column in combination with an evaporative light scattering (ELS) detector. Separation of d-xylonate from other components of the fermentation medium was achieved. The dynamic range of the method was 0.2-7.0 g/L.
[Mh] Termos MeSH primário: Aminoácidos/isolamento & purificação
Cromatografia Líquida de Alta Pressão/métodos
Açúcares Ácidos/isolamento & purificação
[Mh] Termos MeSH secundário: Cromatografia Líquida de Alta Pressão/instrumentação
Difusão Dinâmica da Luz
Fermentação
Limite de Detecção
Saccharomyces cerevisiae/metabolismo
Volatilização
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amino Acids); 0 (Sugar Acids)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170703
[Lr] Data última revisão:
170703
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170510
[St] Status:MEDLINE
[do] DOI:10.1080/09168451.2017.1292839


  6 / 1642 MEDLINE  
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[PMID]:28438125
[Au] Autor:Cram ED; Rockey DD; Dolan BP
[Ad] Endereço:Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University, 105 Magruder Hall, Corvallis, OR, 97331, USA. crame@oregonstate.edu.
[Ti] Título:Chlamydia spp. development is differentially altered by treatment with the LpxC inhibitor LPC-011.
[So] Source:BMC Microbiol;17(1):98, 2017 Apr 24.
[Is] ISSN:1471-2180
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Chlamydia species are obligate intracellular bacteria that infect a broad range of mammalian hosts. Members of related genera are pathogens of a variety of vertebrate and invertebrate species. Despite the diversity of Chlamydia, all species contain an outer membrane lipooligosaccharide (LOS) that is comprised of a genus-conserved, and genus-defining, trisaccharide 3-deoxy-D-manno-oct-2-ulosonic acid Kdo region. Recent studies with lipopolysaccharide inhibitors demonstrate that LOS is important for the C. trachomatis developmental cycle during RB- > EB differentiation. Here, we explore the effects of one of these inhibitors, LPC-011, on the developmental cycle of five chlamydial species. RESULTS: Sensitivity to the drug varied in some of the species and was conserved between others. We observed that inhibition of LOS biosynthesis in some chlamydial species induced formation of aberrant reticulate bodies, while in other species, no change was observed to the reticulate body. However, loss of LOS production prevented completion of the chlamydial reproductive cycle in all species tested. In previous studies we found that C. trachomatis and C. caviae infection enhances MHC class I antigen presentation of a model self-peptide. We find that treatment with LPC-011 prevents enhanced host-peptide presentation induced by infection with all chlamydial-species tested. CONCLUSIONS: The data demonstrate that LOS synthesis is necessary for production of infectious progeny and inhibition of LOS synthesis induces aberrancy in certain chlamydial species, which has important implications for the use of LOS synthesis inhibitors as potential antibiotics.
[Mh] Termos MeSH primário: Proteínas de Bactérias/efeitos dos fármacos
Proteínas de Bactérias/genética
Chlamydia/efeitos dos fármacos
Chlamydia/crescimento & desenvolvimento
Ácidos Hidroxâmicos/antagonistas & inibidores
Treonina/análogos & derivados
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Ampicilina/farmacologia
Animais
Antibacterianos/farmacologia
Linhagem Celular/efeitos dos fármacos
Linhagem Celular/microbiologia
Chlamydia/genética
Chlamydia/patogenicidade
Infecções por Chlamydia/tratamento farmacológico
Citoplasma/microbiologia
Fibroblastos
Regulação Bacteriana da Expressão Gênica/efeitos dos fármacos
Interações Hospedeiro-Patógeno
Seres Humanos
Ácidos Hidroxâmicos/administração & dosagem
Lipopolissacarídeos/biossíntese
Camundongos
Testes de Sensibilidade Microbiana
Fenótipo
Filogenia
Biossíntese de Proteínas/efeitos dos fármacos
Alinhamento de Sequência
Análise de Sequência de Proteína
Açúcares Ácidos
Treonina/administração & dosagem
Treonina/antagonistas & inibidores
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Bacterial Proteins); 0 (Hydroxamic Acids); 0 (LPC-011); 0 (Lipopolysaccharides); 0 (Sugar Acids); 0 (lipid-linked oligosaccharides); 1069-03-0 (2-keto-3-deoxyoctonate); 2ZD004190S (Threonine); 7C782967RD (Ampicillin)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170925
[Lr] Data última revisão:
170925
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170426
[St] Status:MEDLINE
[do] DOI:10.1186/s12866-017-0992-8


  7 / 1642 MEDLINE  
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[PMID]:28417461
[Au] Autor:Alazi E; Khosravi C; Homan TG; du Pré S; Arentshorst M; Di Falco M; Pham TTM; Peng M; Aguilar-Pontes MV; Visser J; Tsang A; de Vries RP; Ram AFJ
[Ad] Endereço:Molecular Microbiology and Biotechnology, Institute of Biology Leiden, Leiden University, The Netherlands.
[Ti] Título:The pathway intermediate 2-keto-3-deoxy-L-galactonate mediates the induction of genes involved in D-galacturonic acid utilization in Aspergillus niger.
[So] Source:FEBS Lett;591(10):1408-1418, 2017 May.
[Is] ISSN:1873-3468
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In Aspergillus niger, the enzymes encoded by gaaA, gaaB, and gaaC catabolize d-galacturonic acid (GA) consecutively into l-galactonate, 2-keto-3-deoxy-l-galactonate, pyruvate, and l-glyceraldehyde, while GaaD converts l-glyceraldehyde to glycerol. Deletion of gaaB or gaaC results in severely impaired growth on GA and accumulation of l-galactonate and 2-keto-3-deoxy-l-galactonate, respectively. Expression levels of GA-responsive genes are specifically elevated in the ∆gaaC mutant on GA as compared to the reference strain and other GA catabolic pathway deletion mutants. This indicates that 2-keto-3-deoxy-l-galactonate is the inducer of genes required for GA utilization.
[Mh] Termos MeSH primário: Aspergillus niger/crescimento & desenvolvimento
Proteínas Fúngicas/genética
Açúcares Ácidos/metabolismo
[Mh] Termos MeSH secundário: Aspergillus niger/enzimologia
Aspergillus niger/genética
Proteínas Fúngicas/metabolismo
Regulação Enzimológica da Expressão Gênica
Redes e Vias Metabólicas
Mutação
[Pt] Tipo de publicação:LETTER
[Nm] Nome de substância:
0 (Fungal Proteins); 0 (Sugar Acids); 13382-27-9 (galactonic acid)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170919
[Lr] Data última revisão:
170919
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170419
[St] Status:MEDLINE
[do] DOI:10.1002/1873-3468.12654


  8 / 1642 MEDLINE  
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[PMID]:28407382
[Au] Autor:Meinert C; Senger J; Witthohn M; Wübbeler JH; Steinbüchel A
[Ad] Endereço:Institut für Molekulare Mikrobiologie und Biotechnologie, Westfälische Wilhelms-Universität, Münster, D-48149, Germany.
[Ti] Título:Carbohydrate uptake in Advenella mimigardefordensis strain DPN7 is mediated by periplasmic sugar oxidation and a TRAP-transport system.
[So] Source:Mol Microbiol;104(6):916-930, 2017 Jun.
[Is] ISSN:1365-2958
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In this study, we investigated an SBP (DctP ) of a tripartite ATP-independent periplasmic transport system (TRAP) in Advenella mimigardefordensis strain DPN7 . Deletion of dctP as well as of the two transmembrane compounds of the tripartite transporter, dctQ and dctM, impaired growth of A. mimigardefordensis strain DPN7 , if cultivated on mineral salt medium supplemented with d-glucose, d-galactose, l-arabinose, d-fucose, d-xylose or d-gluconic acid, respectively. The wild type phenotype was restored during complementation studies of A. mimigardefordensis ΔdctP using the broad host vector pBBR1MCS-5::dctP . Furthermore, an uptake assay with radiolabeled [ C(U)]-d-glucose clearly showed that the deletion of dctP , dctQ and dctM, respectively, disabled the uptake of this aldoses in cells of either mutant strain. Determination of K performing thermal shift assays showed a shift in the melting temperature of DctP in the presence of d-gluconic acid (K 11.76 ± 1.3 µM) and the corresponding aldonic acids to the above-mentioned carbohydrates d-galactonate (K 10.72 ± 1.4 µM), d-fuconic acid (K 13.50 ± 1.6 µM) and d-xylonic acid (K 8.44 ± 1.0 µM). The sugar (glucose) dehydrogenase activity (E.C.1.1.5.2) in the membrane fraction was shown for all relevant sugars, proving oxidation of the molecules in the periplasm, prior to transport.
[Mh] Termos MeSH primário: Alcaligenaceae/metabolismo
Proteínas de Membrana Transportadoras/metabolismo
Açúcares Ácidos/metabolismo
[Mh] Termos MeSH secundário: Alcaligenaceae/genética
Proteínas de Bactérias/genética
Carboidratos
Galactose/metabolismo
Gluconatos/metabolismo
Glucose/metabolismo
Proteínas de Membrana Transportadoras/genética
Periplasma/fisiologia
Propionatos/metabolismo
Análise de Sequência de DNA
Simportadores/metabolismo
Xilose/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Carbohydrates); 0 (Gluconates); 0 (Membrane Transport Proteins); 0 (Propionates); 0 (Sugar Acids); 0 (Symporters); A1TA934AKO (Xylose); IY9XDZ35W2 (Glucose); R4R8J0Q44B (gluconic acid); X2RN3Q8DNE (Galactose)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170921
[Lr] Data última revisão:
170921
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170414
[St] Status:MEDLINE
[do] DOI:10.1111/mmi.13692


  9 / 1642 MEDLINE  
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[PMID]:28407314
[Au] Autor:Thomas GH
[Ad] Endereço:Department of Biology, Wentworth Way, University of York, York, UK, YO10 5DD.
[Ti] Título:On the pull: periplasmic trapping of sugars before transport.
[So] Source:Mol Microbiol;104(6):883-888, 2017 Jun.
[Is] ISSN:1365-2958
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Bacteria have evolved many routes for taking up nutrients, demonstrating great versatility in the types and mechanism of uptake used in different physiological conditions. The discovery of a single transporter in the bacterium Advenella mimigardefordensis for the uptake of five different sugars, including L-glucose and D-xylose, is described in this issue (Meinert et al., ), providing yet another example of the surprising adaptability of bacterial transport strategies. The transporter identified is a tripartite ATP-independent (TRAP) transporter, not previously associated with sugar transport, and in fact does not transport the sugars directly at all, rather requiring them to be converted in the periplasm to their respective sugar acid forms before transport through what appears to be a novel general sugar acid transporter. In this commentary, I describe how this process is consistent with the known mechanisms of TRAP transporters and consider how the role of sugar oxidation, or oxidative fermentation, operates with multiple hexose and pentose sugars. Finally I suggest that the periplasmic conversion of nutrients acquired across the outer membrane, before transport across the inner membrane, could have potentially useful biological functions in Gram negative bacteria.
[Mh] Termos MeSH primário: Transporte Biológico/fisiologia
Açúcares Ácidos/metabolismo
Simportadores/metabolismo
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Bactérias/metabolismo
Proteínas de Bactérias/metabolismo
Glucose
Proteínas de Membrana Transportadoras/metabolismo
Periplasma/metabolismo
Simportadores/fisiologia
Xilose
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Membrane Transport Proteins); 0 (Sugar Acids); 0 (Symporters); A1TA934AKO (Xylose); IY9XDZ35W2 (Glucose)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170921
[Lr] Data última revisão:
170921
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170414
[St] Status:MEDLINE
[do] DOI:10.1111/mmi.13691


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[PMID]:28371606
[Au] Autor:Kasimova AA; Shneider MM; Arbatsky NP; Popova AV; Shashkov AS; Miroshnikov KA; Balaji V; Biswas I; Knirel YA
[Ad] Endereço:Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, 119991, Russia. yknirel@gmail.com.
[Ti] Título:Structure and Gene Cluster of the K93 Capsular Polysaccharide of Acinetobacter baumannii B11911 Containing 5-N-Acetyl-7-N-[(R)-3-hydroxybutanoyl]pseudaminic Acid.
[So] Source:Biochemistry (Mosc);82(4):483-489, 2017 Apr.
[Is] ISSN:1608-3040
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Capsular polysaccharide (CPS) assigned to the K93 type was isolated from the bacterium Acinetobacter baumannii B11911 and studied by sugar analysis along with one- and two-dimensional H and C NMR spectroscopy. The CPS was found to contain a derivative of pseudaminic acid, and the structure of the branched tetrasaccharide repeating unit was established. Genes in the KL93 capsule biosynthesis locus were annotated and found to be consistent with the CPS structure established. The K93 CPS has the α-d-Galp-(1→6)-ß-d-Galp-(1→3)-d-GalpNAc trisaccharide fragment in common with the K14 CPS of Acinetobacter nosocomialis LUH 5541 and A. baumannii D46. It also shares the ß-d-Galp-(1→3)-d-GalpNAc disaccharide fragment and the corresponding predicted Gal transferase Gtr5, as well as the initiating GalNAc-1-P transferase ItrA2, with a number of A. baumannii strains.
[Mh] Termos MeSH primário: Acinetobacter baumannii/metabolismo
Cápsulas Bacterianas/metabolismo
Família Multigênica
Polissacarídeos/química
Polissacarídeos/genética
Açúcares Ácidos/análise
[Mh] Termos MeSH secundário: Acinetobacter baumannii/genética
Configuração de Carboidratos
Espectroscopia de Ressonância Magnética Nuclear de Carbono-13
Genes Bacterianos
Espectroscopia de Prótons por Ressonância Magnética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Polysaccharides); 0 (Sugar Acids)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170531
[Lr] Data última revisão:
170531
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170404
[St] Status:MEDLINE
[do] DOI:10.1134/S0006297917040101



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