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  1 / 3018 MEDLINE  
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[PMID]:29185908
[Au] Autor:Prabhu AA; Venkata Dasu V
[Ad] Endereço:a Department of Biosciences and Bioengineering , Biochemical Engineering Laboratory, Indian Institute of Technology Guwahati , Guwahati , Assam , India.
[Ti] Título:Dual-substrate inhibition kinetic studies for recombinant human interferon gamma producing Pichia pastoris.
[So] Source:Prep Biochem Biotechnol;47(10):953-962, 2017 Nov 26.
[Is] ISSN:1532-2297
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Pichia pastoris is considered as one of the prominent host extensively used as a platform for heterologous protein production. In the present study, the growth inhibition kinetics of recombinant P. pastoris expressing human interferon gamma was studied under different initial substrate concentrations of gluconate (10-100 g L ) and methanol (2-50 g L ) in modified FM22 medium. The highest specific growth rate of 0.0206 and 0.019 hr was observed at 60 g L of gluconate and 10 g L of methanol, respectively. Various three- and four-parametric Monod-variant models were chosen to analyze the inhibition kinetics. The model parameters as well as goodness of fit were estimated using nonlinear regression analysis. The three-parameter Haldane model was found to be best fit for both gluconate (R = 0.95) and methanol substrate (R = 0.96). The parameter sensitivity analysis revealed that µ , K , and K are the most sensitive parameters for both methanol and gluconate. Different substrate inhibition models were fitted to the growth kinetic data and the additive form of double Webb model was found to be the best to explain the growth kinetics of recombinant P. pastoris.
[Mh] Termos MeSH primário: Gluconatos/metabolismo
Microbiologia Industrial/métodos
Interferon gama/metabolismo
Metanol/metabolismo
Pichia/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Meios de Cultura/metabolismo
Seres Humanos
Cinética
Pichia/metabolismo
Proteínas Recombinantes/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Culture Media); 0 (Gluconates); 0 (IFNG protein, human); 0 (Recombinant Proteins); 82115-62-6 (Interferon-gamma); R4R8J0Q44B (gluconic acid); Y4S76JWI15 (Methanol)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171225
[Lr] Data última revisão:
171225
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171130
[St] Status:MEDLINE
[do] DOI:10.1080/10826068.2017.1350977


  2 / 3018 MEDLINE  
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[PMID]:28866974
[Au] Autor:Hammond NE; Bellomo R; Gallagher M; Gattas D; Glass P; Mackle D; Micallef S; Myburgh J; Saxena M; Taylor C; Young P; Finfer S
[Ad] Endereço:Critical Care and Trauma Division, The George Institute for Global Health, Sydney, NSW, Australia.
[Ti] Título:The Plasma-Lyte 148 v Saline (PLUS) study protocol: a multicentre, randomised controlled trial of the effect of intensive care fluid therapy on mortality.
[So] Source:Crit Care Resusc;19(3):239-246, 2017 Sep.
[Is] ISSN:1441-2772
[Cp] País de publicação:Australia
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: 0.9% sodium chloride (saline) is the most commonly administered resuscitation fluid on a global basis but emerging evidence suggests that its high chloride content may have important adverse effects. OBJECTIVE: To describe the study protocol for the Plasma- Lyte 148 v Saline study, which will test the hypothesis that in critically ill adult patients the use of Plasma-Lyte 148 (a buffered crystalloid solution) for fluid therapy results in different 90-day all-cause mortality when compared with saline. DESIGN AND SETTING: We will conduct this multicentre, blinded, randomised controlled trial in approximately 50 intensive care units in Australia and New Zealand. We will randomly assign 8800 patients to either Plasma-Lyte 148 or saline for all resuscitation fluid, maintenance fluid and compatible drug dilution therapy while in the ICU for up to 90 days after randomisation. OUTCOME MEASURES: The primary outcome is 90-day all-cause mortality; secondary outcomes include mean and peak creatinine concentration, incidence of renal replacement therapy, incidence and duration of vasoactive drug treatment, duration of mechanical ventilation, ICU and hospital length of stay, and quality of life and health services use at 6 months. RESULTS AND CONCLUSIONS: The PLUS study will provide high-quality data on the comparative safety and efficacy of Plasma-Lyte 148 compared with saline for resuscitation and compatible crystalloid fluid therapy in critically ill adult patients.
[Mh] Termos MeSH primário: Estado Terminal/terapia
Hidratação/métodos
Cloreto de Sódio/uso terapêutico
[Mh] Termos MeSH secundário: Austrália
Creatinina/metabolismo
Estado Terminal/mortalidade
Gluconatos/uso terapêutico
Serviços de Saúde/utilização
Seres Humanos
Unidades de Terapia Intensiva
Tempo de Internação
Cloreto de Magnésio/uso terapêutico
Mortalidade
Nova Zelândia
Cloreto de Potássio/uso terapêutico
Qualidade de Vida
Terapia de Substituição Renal/utilização
Respiração Artificial
Ressuscitação
Acetato de Sódio/uso terapêutico
Fatores de Tempo
Vasoconstritores/uso terapêutico
Vasodilatadores/uso terapêutico
[Pt] Tipo de publicação:JOURNAL ARTICLE; MULTICENTER STUDY; RANDOMIZED CONTROLLED TRIAL
[Nm] Nome de substância:
0 (Gluconates); 0 (Plasma-lyte 148); 0 (Vasoconstrictor Agents); 0 (Vasodilator Agents); 02F3473H9O (Magnesium Chloride); 451W47IQ8X (Sodium Chloride); 4550K0SC9B (Sodium Acetate); 660YQ98I10 (Potassium Chloride); AYI8EX34EU (Creatinine)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171017
[Lr] Data última revisão:
171017
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170905
[St] Status:MEDLINE


  3 / 3018 MEDLINE  
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[PMID]:28848047
[Au] Autor:Yao P; Sun H; Xu C; Chen T; Zou B; Jiang P; Du W
[Ad] Endereço:From the School of Life Sciences, Tsinghua University, Beijing 100084, China and.
[Ti] Título:Evidence for a direct cross-talk between malic enzyme and the pentose phosphate pathway via structural interactions.
[So] Source:J Biol Chem;292(41):17113-17120, 2017 Oct 13.
[Is] ISSN:1083-351X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Recent studies have revealed that the oxidative entose hosphate athway (PPP), malic enzyme (ME), and folate metabolism are the three major routes for generating cellular NADPH, a key cofactor involved in redox control and reductive biosynthesis. Many tumor cells exhibit altered NADPH metabolism to fuel their rapid proliferation. However, little is known about how NADPH metabolism is coordinated in tumor cells. Here we report that ME1 increases the PPP flux by forming physiological complexes with 6-phosphogluconate dehydrogenase (6PGD). We found that ME1 and 6PGD form a hetero-oligomer that increases the capability of 6PGD to bind its substrate 6-phosphogluconate. Through activating 6PGD, ME1 enhances NADPH generation, PPP flux, and tumor cell growth. Interestingly, although ME1 could bind either the dimer-defect mutant 6PGD (K294R) or the NADP -binding defect 6PGD mutants, only 6PGD (K294R) activity was induced by ME1. Thus, ME1/6PGD hetero-complexes may mimic the active oligomer form of 6PGD. Together, these findings uncover a direct cross-talk mechanism between ME1 and PPP, may reveal an alternative model for signaling transduction via protein conformational simulation, and pave the way for better understanding how metabolic pathways are coordinated in cancer.
[Mh] Termos MeSH primário: Malato Desidrogenase/metabolismo
Proteínas de Neoplasias/metabolismo
Neoplasias/enzimologia
Via de Pentose Fosfato
Multimerização Proteica
Transdução de Sinais
[Mh] Termos MeSH secundário: Linhagem Celular Tumoral
Gluconatos/química
Gluconatos/metabolismo
Seres Humanos
Hidroliases/química
Hidroliases/genética
Hidroliases/metabolismo
Malato Desidrogenase/química
Malato Desidrogenase/genética
Mutação de Sentido Incorreto
NADP/química
NADP/genética
NADP/metabolismo
Proteínas de Neoplasias/química
Proteínas de Neoplasias/genética
Neoplasias/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Gluconates); 0 (Neoplasm Proteins); 53-59-8 (NADP); EC 1.1.1.37 (Malate Dehydrogenase); EC 1.1.1.39 (malate dehydrogenase (decarboxylating)); EC 4.2.1.- (Hydro-Lyases); EC 4.2.1.12 (phosphogluconate dehydratase); W31WK7B8U0 (6-phosphogluconic acid)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171019
[Lr] Data última revisão:
171019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170830
[St] Status:MEDLINE
[do] DOI:10.1074/jbc.M117.810309


  4 / 3018 MEDLINE  
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[PMID]:28724257
[Au] Autor:Zhou X; Zhou X; Huang L; Cao R; Xu Y
[Ad] Endereço:Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing 210037, People's Republic of China; College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, People's Republic of China; Jiangsu Province Key Laboratory
[Ti] Título:Efficient coproduction of gluconic acid and xylonic acid from lignocellulosic hydrolysate by Zn(II)-selective inhibition on whole-cell catalysis by Gluconobacter oxydans.
[So] Source:Bioresour Technol;243:855-859, 2017 Nov.
[Is] ISSN:1873-2976
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:With Zn(II)-selective inhibition on the whole-cell catalysis of Gluconobacter oxydans NL71, gluconic acid and xylonic acid were coproduced efficiently from the hydrolysate of corn stover. Further metabolism of gluconic acid to the by-product 2-ketogluconic acid was prevented by addition of 10g/L ZnCl . Remarkably, yields of 93.91% of gluconic acid and 93.36% of xylonic acid were obtained with the supplement of ZnCl in the synthetic medium, without by-product production. After optimization of the concentrations of ZnCl and inocula of the strain, maximum amounts of gluconic acid and xylonic acid were coproduced at titers of 63.01g/L and 33.81g/L, with an overall utilization of 100% of the sugars in the enzymatic hydrolysate of corn stover. The results showed execution of our objective to prove this novel bioconversion method for simultaneously producing gluconic acid and xylonic acid, which would benefit subsequent studies on the comprehensive utilization of lignocellulosic materials.
[Mh] Termos MeSH primário: Gluconatos
Gluconobacter oxydans
[Mh] Termos MeSH secundário: Catálise
Fermentação
Zinco
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Gluconates); J41CSQ7QDS (Zinc); R4R8J0Q44B (gluconic acid)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171023
[Lr] Data última revisão:
171023
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170721
[St] Status:MEDLINE


  5 / 3018 MEDLINE  
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[PMID]:28686351
[Au] Autor:Kamio M; Nagakura Y; Yano H
[Ad] Endereço:Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato, Tokyo, 108-8477, Japan.
[Ti] Título:The Molting Biomarker Molecule Exists as 2-Acetamido-2-deoxy-gluconic Acid in Urine of Blue Crabs and Helmet Crabs.
[So] Source:Chem Biodivers;14(9), 2017 Sep.
[Is] ISSN:1612-1880
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:N-Acetyl-d-glucosamino-1,5-lactone 1 has been reported as a candidate component of the sex pheromone mixture of female blue crabs, Callinectes sapidus, since it is present in the urine of reproductive females and males detect it. Theoretically, 1 can convert to a 1,4-lactone isomer 2 or to the corresponding carboxylic acid, 2-acetamido-2-deoxygluconic acid 3 by hydrolysis in aqueous solution. In this study, we examined the biologically relevant state of equilibrium mixture of 1, 2, and 3 in crab urine using ESI-MS and NMR analyses. The ESI-MS analysis showed that the dominant form of solubilized synthetic 1 is lactone 1 and/or 2, immediately after solubilization in deuterated water, seawater, and phosphate buffer and gradually changing to carboxylic acid 3 which becomes most predominant in phosphate buffer. The NMR analysis showed that synthetic 1 converts to other forms in deuterated water and seawater, and reaches an equilibrium mixture of at least three forms within 24 h. In contrast, 1 converts to a single state of another form in deuterated water with 35 mm phosphate buffer pH 7.6 within 24 h, which is identical to the state in urine with or without phosphate buffer. Thus, we conclude that the molting biomarker sensed by male crabs is 3.
[Mh] Termos MeSH primário: Acetamidas/análise
Braquiúros/crescimento & desenvolvimento
Gluconatos/análise
Muda
[Mh] Termos MeSH secundário: Animais
Braquiúros/química
Feminino
Lactonas/análise
Espectroscopia de Ressonância Magnética
Masculino
Atrativos Sexuais/análise
Espectrometria de Massas por Ionização por Electrospray
Urina/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acetamides); 0 (Gluconates); 0 (Lactones); 0 (Sex Attractants); 3442-69-1 (2-deoxygluconic acid)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171006
[Lr] Data última revisão:
171006
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170708
[St] Status:MEDLINE
[do] DOI:10.1002/cbdv.201700063


  6 / 3018 MEDLINE  
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[PMID]:28667014
[Au] Autor:Corkins ME; Wilson S; Cocuron JC; Alonso AP; Bird AJ
[Ad] Endereço:From the Department of Molecular Genetics.
[Ti] Título:The gluconate shunt is an alternative route for directing glucose into the pentose phosphate pathway in fission yeast.
[So] Source:J Biol Chem;292(33):13823-13832, 2017 Aug 18.
[Is] ISSN:1083-351X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Glycolysis and the pentose phosphate pathway both play a central role in the degradation of glucose in all domains of life. Another metabolic route that can facilitate glucose breakdown is the gluconate shunt. In this shunt glucose dehydrogenase and gluconate kinase catalyze the two-step conversion of glucose into the pentose phosphate pathway intermediate 6-phosphogluconate. Despite the presence of these enzymes in many organisms, their only established role is in the production of 6-phosphogluconate for the Entner-Doudoroff pathway. In this report we performed metabolic profiling on a strain of lacking the zinc-responsive transcriptional repressor Loz1 with the goal of identifying metabolic pathways that were altered by cellular zinc status. This profiling revealed that Δ cells accumulate higher levels of gluconate. We show that the altered gluconate levels in Δ cells result from increased expression of By analyzing the activity of recombinant Gcd1 and by measuring gluconate levels in strains lacking enzymes of the gluconate shunt we demonstrate that Gcd1 encodes a novel NADP -dependent glucose dehydrogenase that acts in a pathway with the Idn1 gluconate kinase. We also find that cells lacking and , which encode the first enzyme in the pentose phosphate pathway, have a more severe growth phenotype than cells lacking We propose that in Gcd1 and Idn1 act together to shunt glucose into the pentose phosphate pathway, creating an alternative route for directing glucose into the pentose phosphate pathway that bypasses hexokinase and the rate-limiting enzyme glucose-6-phosphate dehydrogenase.
[Mh] Termos MeSH primário: Glucose Desidrogenase/metabolismo
Glucosefosfato Desidrogenase/metabolismo
Via de Pentose Fosfato
Fosfotransferases (Aceptor do Grupo Álcool)/metabolismo
Proteínas de Schizosaccharomyces pombe/metabolismo
Schizosaccharomyces/enzimologia
Fatores de Transcrição/metabolismo
[Mh] Termos MeSH secundário: Metabolismo Energético
Deleção de Genes
Gluconatos/metabolismo
Glucose Desidrogenase/genética
Glucosefosfato Desidrogenase/genética
Metabolômica/métodos
Fosfotransferases (Aceptor do Grupo Álcool)/genética
Proteínas Recombinantes/metabolismo
Schizosaccharomyces/crescimento & desenvolvimento
Schizosaccharomyces/metabolismo
Proteínas de Schizosaccharomyces pombe/genética
Fatores de Transcrição/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Gluconates); 0 (Loz1 protein, S pombe); 0 (Recombinant Proteins); 0 (Schizosaccharomyces pombe Proteins); 0 (Transcription Factors); EC 1.1.1.- (Gcd1 protein, S pombe); EC 1.1.1.- (Glucose Dehydrogenases); EC 1.1.1.49 (Glucosephosphate Dehydrogenase); EC 1.1.1.49 (Zwf1 protein, S pombe); EC 2.7.1.- (Idn1 protein, S pombe); EC 2.7.1.- (Phosphotransferases (Alcohol Group Acceptor)); EC 2.7.1.12 (gluconokinase); R4R8J0Q44B (gluconic acid)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170702
[St] Status:MEDLINE
[do] DOI:10.1074/jbc.M117.798488


  7 / 3018 MEDLINE  
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[PMID]:28658302
[Au] Autor:Campilongo R; Fung RKY; Little RH; Grenga L; Trampari E; Pepe S; Chandra G; Stevenson CEM; Roncarati D; Malone JG
[Ad] Endereço:John Innes Centre, Norwich Research Park, Colney Lane, Norwich, United Kingdom.
[Ti] Título:One ligand, two regulators and three binding sites: How KDPG controls primary carbon metabolism in Pseudomonas.
[So] Source:PLoS Genet;13(6):e1006839, 2017 Jun.
[Is] ISSN:1553-7404
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Effective regulation of primary carbon metabolism is critically important for bacteria to successfully adapt to different environments. We have identified an uncharacterised transcriptional regulator; RccR, that controls this process in response to carbon source availability. Disruption of rccR in the plant-associated microbe Pseudomonas fluorescens inhibits growth in defined media, and compromises its ability to colonise the wheat rhizosphere. Structurally, RccR is almost identical to the Entner-Doudoroff (ED) pathway regulator HexR, and both proteins are controlled by the same ED-intermediate; 2-keto-3-deoxy-6-phosphogluconate (KDPG). Despite these similarities, HexR and RccR control entirely different aspects of primary metabolism, with RccR regulating pyruvate metabolism (aceEF), the glyoxylate shunt (aceA, glcB, pntAA) and gluconeogenesis (pckA, gap). RccR displays complex and unusual regulatory behaviour; switching repression between the pyruvate metabolism and glyoxylate shunt/gluconeogenesis loci depending on the available carbon source. This regulatory complexity is enabled by two distinct pseudo-palindromic binding sites, differing only in the length of their linker regions, with KDPG binding increasing affinity for the 28 bp aceA binding site but decreasing affinity for the 15 bp aceE site. Thus, RccR is able to simultaneously suppress and activate gene expression in response to carbon source availability. Together, the RccR and HexR regulators enable the rapid coordination of multiple aspects of primary carbon metabolism, in response to levels of a single key intermediate.
[Mh] Termos MeSH primário: Proteínas de Bactérias/genética
Gluconatos/metabolismo
Pseudomonas fluorescens/genética
Fatores de Transcrição/genética
[Mh] Termos MeSH secundário: Sítios de Ligação
Carbono/metabolismo
Regulação Bacteriana da Expressão Gênica
Gluconeogênese/genética
Glucose/metabolismo
Glioxilatos/metabolismo
Ligantes
Redes e Vias Metabólicas/genética
Pseudomonas fluorescens/metabolismo
Ácido Pirúvico/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Gluconates); 0 (Glyoxylates); 0 (Ligands); 0 (Transcription Factors); 27244-54-8 (2-keto-3-deoxy-6-phosphogluconate); 7440-44-0 (Carbon); 8558G7RUTR (Pyruvic Acid); IY9XDZ35W2 (Glucose); JQ39C92HH6 (glyoxylic acid)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170726
[Lr] Data última revisão:
170726
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170629
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pgen.1006839


  8 / 3018 MEDLINE  
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[PMID]:28651514
[Au] Autor:Zampieri FG; Azevedo LCP; Corrêa TD; Falavigna M; Machado FR; Assunção MSC; Lobo SMA; Dourado LK; Berwanger O; Kellum JA; Brandão N; Cavalcanti AB; BaSICS Investigators and the BRICNet
[Ad] Endereço:Research Institute HCor, Hospital of Coração, São Paulo, Brazil. abiasi@hcor.com.br.
[Ti] Título:Study protocol for the Balanced Solution versus Saline in Intensive Care Study (BaSICS): a factorial randomised trial.
[So] Source:Crit Care Resusc;19(2):175-182, 2017 Jun.
[Is] ISSN:1441-2772
[Cp] País de publicação:Australia
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The effectiveness and safety of balanced crystalloid fluids compared with saline (0.9% sodium chloride) as a fluid of choice in critically ill patients remain unclear. The effects of different fluid infusion rates on outcomes are also unknown. OBJECTIVES: To test the hypothesis that a balanced crystalloid solution, compared with saline, decreases 90-day all-cause mortality among critically ill patients; and to test the hypothesis that slow, compared with rapid, infusion rate decreases 90-day mortality in this population of patients. METHODS: The Balanced Solution versus Saline in Intensive Care Study (BaSICS) is a pragmatic, 2 ??2 factorial, randomised controlled trial. A total of 11 000 patients will be recruited from at least 100 Brazilian intensive care units. Patients will be randomised to receive Plasma-Lyte 148 or saline, and to rapid infusion (999 mL/h) or slow infusion (333 mL/h). Study fluids will be used for resuscitation episodes (at rapid or slow infusion rates), dilution of compatible medications and maintenance solutions. Patients, health care providers and investigators will be blinded to the solutions being tested. The rate of bolus infusion will not be blinded. OUTCOMES: The primary outcome is 90-day all-cause mortality. Secondary outcomes are: incidence of renal failure requiring renal replacement therapy within 90 days, incidence of acute kidney injury (Kidney Disease: Improving Global Outcomes stages 2 and 3), incidence of non-renal organ dysfunction assessed by Sepsis-related Organ Failure Assessment score at Days 3 and 7, and number of mechanical ventilationfree days within the first 28 days after randomisation. RESULTS AND CONCLUSIONS: The BaSICS trial will provide robust evidence on whether a balanced crystalloid, compared with saline, improves important patient outcomes in critically ill patients. BaSICS will also provide relevant information on whether bolus infusion rate affects outcomes in this population. TRIAL REGISTRATION: ClinicalTrials.gov NCT02875873.
[Mh] Termos MeSH primário: Estado Terminal/mortalidade
Estado Terminal/terapia
Hidratação/métodos
Unidades de Terapia Intensiva
Cloreto de Sódio/administração & dosagem
[Mh] Termos MeSH secundário: Idoso
Brasil
Causas de Morte
Método Duplo-Cego
Gluconatos/administração & dosagem
Mortalidade Hospitalar
Seres Humanos
Infusões Intravenosas/métodos
Cloreto de Magnésio/administração & dosagem
Seleção de Pacientes
Cloreto de Potássio/administração & dosagem
Projetos de Pesquisa
Acetato de Sódio/administração & dosagem
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; MULTICENTER STUDY; RANDOMIZED CONTROLLED TRIAL
[Nm] Nome de substância:
0 (Gluconates); 0 (Plasma-lyte 148); 02F3473H9O (Magnesium Chloride); 451W47IQ8X (Sodium Chloride); 4550K0SC9B (Sodium Acetate); 660YQ98I10 (Potassium Chloride)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170901
[Lr] Data última revisão:
170901
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170628
[St] Status:MEDLINE


  9 / 3018 MEDLINE  
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[PMID]:28516852
[Au] Autor:Nakata H; Tsubotani Y; Nii T; Hagi A; Inoue Y; Imamura T
[Ad] Endereço:1​Naruto Research Institute, Research and Development Center, Otsuka Pharmaceutical Factory, Inc., Tokushima, Japan.
[Ti] Título:Effects of olanexidine gluconate on preoperative skin preparation: an experimental study in cynomolgus monkeys.
[So] Source:J Med Microbiol;66(5):678-685, 2017 May.
[Is] ISSN:1473-5644
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:PURPOSE: To determine the bactericidal efficacy of a new topical antiseptic for preoperative skin preparation, olanexidine gluconate (development code: OPB-2045G), against transient or resident bacterial flora on the skin of cynomolgus monkeys. METHODOLOGY: After measuring baseline bacterial counts on test sites marked on the abdomens, we applied olanexidine, chlorhexidine or povidone-iodine. After 10 min (fast-acting effect) and 6 h (long-lasting effect), bacterial counts were measured again and log10 reductions were calculated. In addition, we determined the bactericidal effects on the skin contaminated with blood before or after applying the antiseptics. RESULTS: In the non-blood-contaminated condition, the mean log10 reductions of olanexidine at doses of 1-2 % were significantly higher than those of saline (negative control), but did not significantly differ from those of 0.5 % chlorhexidine and 10 % povidone-iodine at either time point. But olanexidine was significantly more effective at both time points than chlorhexidine and povidone-iodine when applied after the site was contaminated with blood. Olanexidine was also significantly more effective than chlorhexidine and as effective as or more effective than povidone-iodine at both time points when skin was contaminated with blood after the antiseptics were applied. CONCLUSION: The bactericidal effects of olanexidine were comparable to those of commercial antiseptics such as chlorhexidine and povidone-iodine in non-blood-contaminated conditions. More importantly, the effect of olanexidine was hardly affected by blood unlike commercial antiseptics. Thus, it is considered that olanexidine has a favourable property for skin preparation in various types of surgical treatments.
[Mh] Termos MeSH primário: Anti-Infecciosos Locais/administração & dosagem
Biguanidas/administração & dosagem
Gluconatos/administração & dosagem
Glucuronatos/administração & dosagem
Cuidados Pré-Operatórios
Pele/microbiologia
Infecção da Ferida Cirúrgica/prevenção & controle
[Mh] Termos MeSH secundário: Acinetobacter baumannii/efeitos dos fármacos
Administração Tópica
Animais
Anti-Infecciosos Locais/química
Biguanidas/química
Clorexidina
Gluconatos/química
Glucuronatos/química
Macaca fascicularis
Povidona-Iodo
Staphylococcus aureus/efeitos dos fármacos
Staphylococcus epidermidis/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Infective Agents, Local); 0 (Biguanides); 0 (Gluconates); 0 (Glucuronates); 0 (olanexidine gluconate); 85H0HZU99M (Povidone-Iodine); 92C2328G7P (olanexidine); R4KO0DY52L (Chlorhexidine)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170519
[St] Status:MEDLINE
[do] DOI:10.1099/jmm.0.000462


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[PMID]:28439964
[Au] Autor:Zheng K; Shen N; Chen H; Ni S; Zhang T; Hu M; Wang J; Sun L; Yang X
[Ad] Endereço:Department of Biochemistry, School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou, 325035, China.
[Ti] Título:Global and targeted metabolomics of synovial fluid discovers special osteoarthritis metabolites.
[So] Source:J Orthop Res;35(9):1973-1981, 2017 Sep.
[Is] ISSN:1554-527X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:To identify special metabolites in synovial fluid of osteoarthritis (OA) via a metabolomics approach. Synovial fluid of 35 participants (25 OA patients and 10 controls) was detected by GC-TOF/MS and multivariate data analysis was applied to analyze correlation among the observations. Different metabolites were screened by VIP value (VIP > 1), student t-test (p < 0.05), and fold change (fold >1.5), and verified with the standard metabolites in the synovial fluid of 24 OA patients and 11 controls by LC/MS. The classification performance of different metabolites was analyzed by receiver operating characteristic (ROC) analysis. The results showed that six different metabolites (glutamine, 1,5-anhydroglucitol, gluconic lactone, tyramine, threonine, and 8-aminocaprylic acid) were strongly associated with OA in global metabolomics. Verified results of the first three metabolites were the same as the identified results using targeted metabolomics. ROC curve analysis demonstrated that their concentrations in synovial fluid were strongly correlated to OA. In addition, the concentrations of gluconic lactone were significantly different between OA and RA. Metabolites with altered levels may be contributors to OA pathogenesis and can be used as potential diagnosis criteria for OA. Gluconic lactone may prove to be a novel criterion for differential diagnosis of OA from RA. © 2017 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 35:1973-1981, 2017.
[Mh] Termos MeSH primário: Metaboloma
Osteoartrite do Joelho/metabolismo
Líquido Sinovial/metabolismo
[Mh] Termos MeSH secundário: Artrite Reumatoide/metabolismo
Estudos de Casos e Controles
Desoxiglucose/metabolismo
Feminino
Gluconatos/metabolismo
Glutamina/metabolismo
Seres Humanos
Lactonas/metabolismo
Masculino
Metabolômica
Meia-Idade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Gluconates); 0 (Lactones); 0RH81L854J (Glutamine); 54BB3B7XMZ (1,5-anhydroglucitol); 9G2MP84A8W (Deoxyglucose); WQ29KQ9POT (beta-glucono-1,5-lactone)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171010
[Lr] Data última revisão:
171010
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170426
[St] Status:MEDLINE
[do] DOI:10.1002/jor.23482



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