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[PMID]: 29236389 [Au] Autor: Holota YV; Holubenko OO; Ostapchuk AM; Serhiychuk TM; Zakordonets LV; Tolstanova GM [Ti] Título: Fecal short-chain fatty acids at different time points after ceftriaxone administration in rats. [So] Source: Ukr Biochem J;89(1):50-8, 2017 Jan-Feb. [Is] ISSN: 2409-4943 [Cp] País de publicação: Ukraine [La] Idioma: eng [Ab] Resumo: Short-chain fatty acids (SCFAs) are major products of the microbial fermentation of dietary fiber in the colon. Recent studies suggest that these products of microbial metabolism in the gut act as signaling molecules, influence host energy homeostasis and play major immunological roles. In the present study, defined the long-term effects of ceftriaxone administration on the fecal SCFAs concentration in Wistar rats. Ceftriaxone (300 mg/kg, i.m.) was administered daily for 14 days. Rats were euthanized in 1, 15 and 56 days after ceftriaxone withdrawal. Caecal weight and fecal concentration of SCFAs by gas chromatography were measured. Ceftriaxone administration induced time-dependent rats' caecal enlargement through accumulation of undigestable substances. In 1 day after ceftriaxone withdrawal, the concentrations of acetic, propionic, butyric acids and total SCFAs were decreased 2.9-, 13.8-, 8.5-, 4.8-fold (P < 0.05), respectively. Concentration of valeric, isovaleric and caproic acids was below the detectable level. That was accompanied by decreased 4.3-fold anaerobic index and increased the relative amount of acetic acid (P < 0.05). In 56 days, concentration of SCFAs was still below control value but higher than in 1 day (except propionic acid). Anaerobic index was lower 1.3-fold (P < 0.05) vs. control. Conclusion: antibiotic therapy induced long-term disturbance in colonic microbiota metabolic activity. [Mh] Termos MeSH primário: Antibacterianos/farmacologia Ceftriaxona/farmacologia Colo/efeitos dos fármacos Ácidos Graxos Voláteis/metabolismo Fezes/química [Mh] Termos MeSH secundário: Ácido Acético/metabolismo Animais Butiratos/metabolismo Caproatos/metabolismo Colo/metabolismo Esquema de Medicação Ácidos Graxos Voláteis/antagonistas & inibidores Injeções Intramusculares Masculino Ácidos Pentanoicos/metabolismo Propionatos/metabolismo Ratos Ratos Wistar Fatores de Tempo [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Anti-Bacterial Agents); 0 (Butyrates); 0 (Caproates); 0 (Fatty Acids, Volatile); 0 (Pentanoic Acids); 0 (Propionates); 1BR7X184L5 (isovaleric acid); 1F8SN134MX (hexanoic acid); 75J73V1629 (Ceftriaxone); GZK92PJM7B (n-pentanoic acid); JHU490RVYR (propionic acid); Q40Q9N063P (Acetic Acid) [Em] Mês de entrada: 1801 [Cu] Atualização por classe: 180116 [Lr] Data última revisão: 180116 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 171214 [St] Status: MEDLINE [do] DOI: 10.15407/ubj89.01.050

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[PMID]: 28467840 [Au] Autor: Frutos OD; Cortes I; Cantera S; Arnaiz E; Lebrero R; Muñoz R [Ad] Endereço: Department of Chemical Engineering and Environmental Technology, University of Valladolid, Dr. Mergelina , s/n, 47011, Valladolid, Spain. [Ti] Título: Nitrous Oxide Abatement Coupled with Biopolymer Production As a Model GHG Biorefinery for Cost-Effective Climate Change Mitigation. [So] Source: Environ Sci Technol;51(11):6319-6325, 2017 Jun 06. [Is] ISSN: 1520-5851 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: N O represents ∼6% of the global greenhouse gas emission inventory and the most important O -depleting substance emitted in this 21st century. Despite its environmental relevance, little attention has been given to cost-effective and environmentally friendly N O abatement methods. Here we examined, the potential of a bubble column (BCR) and an internal loop airlift (ALR) bioreactors of 2.3 L for the abatement of N O from a nitric acid plant emission. The process was based on the biological reduction of N O by Paracoccus denitrificans using methanol as a carbon/electron source. Two nitrogen limiting strategies were also tested for the coproduction of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) coupled with N O reduction. High N O removal efficiencies (REs) (≈87%) together with a low PHBV cell accumulation were observed in both bioreactors in excess of nitrogen. However, PHBV contents of 38-64% were recorded under N limiting conditions along with N O-REs of ≈57% and ≈84% in the ALR and BCR, respectively. Fluorescence in situ hybridization analyses showed that P. denitrificans was dominant (>50%) after 6 months of experimentation. The successful abatement of N O concomitant with PHBV accumulation confirmed the potential of integrating biorefinery concepts into biological gas treatment for a cost-effective GHG mitigation. [Mh] Termos MeSH primário: Biopolímeros Mudança Climática Óxido Nitroso [Mh] Termos MeSH secundário: Reatores Biológicos Hibridização in Situ Fluorescente Ácidos Pentanoicos [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Biopolymers); 0 (Pentanoic Acids); 10237-77-1 (beta-hydroxyvaleric acid); K50XQU1029 (Nitrous Oxide) [Em] Mês de entrada: 1712 [Cu] Atualização por classe: 171215 [Lr] Data última revisão: 171215 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170504 [St] Status: MEDLINE [do] DOI: 10.1021/acs.est.7b00643

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[PMID]: 28844930 [Au] Autor: Peron G; Sut S; Pellizzaro A; Brun P; Voinovich D; Castagliuolo I; Dall'Acqua S [Ad] Endereço: Department of Pharmaceutical and Pharmacological Sciences, University of Padova, Via Marzolo 5, 35131 Padova, Italy. [Ti] Título: The antiadhesive activity of cranberry phytocomplex studied by metabolomics: Intestinal PAC-A metabolites but not intact PAC-A are identified as markers in active urines against uropathogenic Escherichia coli. [So] Source: Fitoterapia;122:67-75, 2017 Oct. [Is] ISSN: 1873-6971 [Cp] País de publicação: Netherlands [La] Idioma: eng [Ab] Resumo: Cranberry procyanidins and quercetin derivatives are considered possible active compounds against urinary tract infections (UTIs). In this paper a small group (n=6) of healthy subjects consumed a product containing 360mg of cranberry extract (42.6% w/w of PAC-A and 14.6% w/w of PAC-B) and 200mg of quercetin. Urine samples were collected after 2,4,6,8, and 24h. The changes in antiadhesive properties against urophatogenic E. coli of the urinary output were determined in vitro and modification to urinary metabolome were studied by LC-MS. Significant antiadhesive properties of urine samples were observed, with the greatest effect 6-8h after oral administration, confirming the possible usefulness of cranberry containing products in urinary tract infections (UTI). Metabolomic analysis revealed that valeric acid and valerolactone derivatives that were detected in 6 and 8h sample, while 4-hydroxy-5-(phenyl)-valeric acid-O-glucuronide and 5-(3',4'-dihydroxyphenyl)-γ-valerolactone at 6h and 4-hydroxy-5-(phenyl)-valeric acid-O-sulphate, 3-hydroxyphenyl-valeric acid, 5-(4'-hydroxyphenyl)-gamma-valerolactone-4'-O-glucuronide and 4-hydroxy-5-(3'-hydroxyphenyl)-valeric acid-3'-O-sulphate were the most abundant at 8h. The present study shows that the antiadhesive properties of urine sample after cranberry consumption are not ascribable to the direct effect of PAC-A, because their levels in urinary output are in the range of ng/mL. On the other hand, significant metabolites that were detected are mainly metabolites of intestinal action on polyphenols and PACs, as well as glucuronidated and sulphated quercetin, suggesting an important role of intestinal modification of phytoconstituents in the cranberry extract mechanism of action. [Mh] Termos MeSH primário: Aderência Bacteriana/efeitos dos fármacos Metaboloma Extratos Vegetais/farmacologia Escherichia coli Uropatogênica/efeitos dos fármacos Vaccinium macrocarpon/química [Mh] Termos MeSH secundário: Adulto Feminino Seres Humanos Intestinos/metabolismo Lactonas/farmacologia Masculino Metabolômica Ácidos Pentanoicos/farmacologia Polifenóis/farmacologia Proantocianidinas/farmacologia Quercetina/farmacologia Infecções Urinárias/tratamento farmacológico Urina/química Adulto Jovem [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Lactones); 0 (Pentanoic Acids); 0 (Plant Extracts); 0 (Polyphenols); 0 (Proanthocyanidins); 9IKM0I5T1E (Quercetin); GZK92PJM7B (n-pentanoic acid); O7056XK37X (gamma-valerolactone) [Em] Mês de entrada: 1711 [Cu] Atualização por classe: 171103 [Lr] Data última revisão: 171103 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170829 [St] Status: MEDLINE

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[PMID]: 28711352 [Au] Autor: Tong L; Kim SH; Chen L; Kosinski A; Shankar BB; Girijavallabhan V; Yang DY; Yu W; Zhou G; Shih NY; Chen S; Hu M; Lundell D; Niu X; Umland S; Kozlowski JA [Ad] Endereço: Department of Medicinal Chemistry, Merck & Co., Inc., 2000 Galloping Hill Road, Kenilworth, NJ 07033, USA. Electronic address: ling.tong@merck.com. [Ti] Título: Development of a prodrug of hydantoin based TACE inhibitor. [So] Source: Bioorg Med Chem Lett;27(16):3704-3708, 2017 08 15. [Is] ISSN: 1464-3405 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: Our research on hydantoin based TNF-α converting enzyme (TACE) inhibitors led to fused bi-heteroaryl hydantoin series that demonstrate sub-nanomolar potency (Ki) as well as excellent activity in human whole blood (hWBA). However, lead compound 2 posed some formulation challenges which prevented it for further development. A prodrug approach was investigated to address this issue. The pivalate prodrug 3 can be formulated as stable neutral form and demonstrated improved DMPK properties when compared with parent compound. [Mh] Termos MeSH primário: Proteína ADAM17/antagonistas & inibidores Hidantoínas/química Hidantoínas/síntese química Hidantoínas/farmacologia Ácidos Pentanoicos/química Pró-Fármacos/síntese química Pró-Fármacos/farmacologia [Mh] Termos MeSH secundário: Proteína ADAM17/metabolismo Administração Oral Animais Área Sob a Curva Cães Ativação Enzimática/efeitos dos fármacos Meia-Vida Haplorrinos Seres Humanos Hidantoínas/administração & dosagem Hidantoínas/farmacocinética Ácidos Pentanoicos/administração & dosagem Ácidos Pentanoicos/farmacocinética Pró-Fármacos/administração & dosagem Pró-Fármacos/farmacocinética Curva ROC Ratos Relação Estrutura-Atividade [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Hydantoins); 0 (Pentanoic Acids); 0 (Prodrugs); 813RE8BX41 (pivalic acid); EC 3.4.24.86 (ADAM17 Protein) [Em] Mês de entrada: 1709 [Cu] Atualização por classe: 171125 [Lr] Data última revisão: 171125 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170717 [St] Status: MEDLINE

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[PMID]: 28692918 [Au] Autor: Gahlawat G; Soni SK [Ad] Endereço: Department of Microbiology, Panjab University, Chandigarh 160014, India. Electronic address: geeta.gahlawat@gmail.com. [Ti] Título: Valorization of waste glycerol for the production of poly (3-hydroxybutyrate) and poly (3-hydroxybutyrate-co-3-hydroxyvalerate) copolymer by Cupriavidus necator and extraction in a sustainable manner. [So] Source: Bioresour Technol;243:492-501, 2017 Nov. [Is] ISSN: 1873-2976 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: Glycerol is a by-product of many industrial processes and huge amounts of it are generated in the form of waste, thereby necessitating a search for the method of its disposal. An interesting solution is the valorization of crude glycerol into value added product such as polyhydroxyalkanoates (PHAs). The feasibility of producing PHAs by Cupriavidus necator was evaluated using crude glycerol (WG). Various cultivation strategies were designed for the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) copolymer by adding different organic acids as precursors at different concentrations levels. Batch cultivation of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) production showed accumulation of 6.76g/L biomass containing 4.84g/L copolymer on WG with a maximum 3-hydroxyvalerate content of 24.6mol%. PHAs extraction using a non-toxic and recyclable solvent, 1,2 propylene carbonate, showed the highest recovery yield (90%) and purity (93%) at 120°C temperature and 30min incubation. This is the first report on jatropha based glycerol valorization for poly(3-hydroxybutyrate-co-3-hydroxyvalerate) production coupled with extraction using non-toxic solvent. [Mh] Termos MeSH primário: Ácido 3-Hidroxibutírico Cupriavidus necator [Mh] Termos MeSH secundário: Glicerol Ácidos Pentanoicos Poliésteres Eliminação de Resíduos Líquidos [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Pentanoic Acids); 0 (Polyesters); 10237-77-1 (beta-hydroxyvaleric acid); PDC6A3C0OX (Glycerol); TZP1275679 (3-Hydroxybutyric Acid) [Em] Mês de entrada: 1710 [Cu] Atualização por classe: 171023 [Lr] Data última revisão: 171023 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170711 [St] Status: MEDLINE

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[PMID]: 28601768 [Au] Autor: Zhang S; Sun X; Fan Y; Qiu T; Gao M; Wang X [Ad] Endereço: College of Life Science, Northeast Forestry University, Harbin 150040, China; Beijing Agro-Biotechnology Research Center, Beijing Key Laboratory of Agricultural Genetic Resources and Biotechnology, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China. [Ti] Título: Heterotrophic nitrification and aerobic denitrification by Diaphorobacter polyhydroxybutyrativorans SL-205 using poly(3-hydroxybutyrate-co-3-hydroxyvalerate) as the sole carbon source. [So] Source: Bioresour Technol;241:500-507, 2017 Oct. [Is] ISSN: 1873-2976 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: A new strain of Diaphorobacter polyhydroxybutyrativorans (strain SL-205) was recently isolated and identified. SL-205 can utilize nitrate and nitrite for denitrification and ammonium for nitrification using poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) as the carbon source under aerobic conditions. SL-205 removed 99.11% of NH -derived N (83.90mg/L), 95.02% of NO -N (308.24mg/L), and 84.13% of NO -N (211.70mg/L), with average removal rates of 1.73mg NH -N/(L·h), 6.10mg NO -N/(L·h), and 4.95mg NO -N/(L·h). Nitrogen gas was the primary end-product, with negligible nitrous oxide accumulation during ammonium removal, accounting for 57.85% of the removed NH -N and 52.30% of the initial NH -N. Moreover, hydroxylamine oxidoreductase, nitrate reductase, and nitrite reductase were detected, further indicating that strain SL-205 underwent heterotrophic nitrification coupled with aerobic denitrification (NH →NH OH→NO →NO →NO →N O→N ). These results support the use of PHBV as a carbon source for nitrogen removal from water and wastewater by strain SL-205. [Mh] Termos MeSH primário: Ácido 3-Hidroxibutírico Carbono Desnitrificação [Mh] Termos MeSH secundário: Aerobiose Processos Heterotróficos Nitrificação Ácidos Pentanoicos [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Pentanoic Acids); 10237-77-1 (beta-hydroxyvaleric acid); 7440-44-0 (Carbon); TZP1275679 (3-Hydroxybutyric Acid) [Em] Mês de entrada: 1711 [Cu] Atualização por classe: 171106 [Lr] Data última revisão: 171106 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170612 [St] Status: MEDLINE

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[PMID]: 28561638 [Au] Autor: Huss AR; Fuller JC; Centrella W; Marshall DL; Deliephan A; Jones CK [Ad] Endereço: 1 Grain Science and Industry, Kansas State University, 201 Shellenberger Hall, Manhattan, Kansas 66506. [Ti] Título: Mitigation of Salmonella on Pet Food Kibbles by Using Liquid and Powdered 3-Hydroxy-3-Methylbutyric Acid. [So] Source: J Food Prot;80(7):1080-1084, 2017 Jul. [Is] ISSN: 1944-9097 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: In recent years, several pet food recalls have been attributed to Salmonella contamination. In addition to the negative impacts on animal health, Salmonella-contaminated pet foods have been linked to infection in humans. With that in mind, the U.S. Food and Drug Administration has set forth a zero-tolerance policy for Salmonella in pet foods. Typically, pet foods are extruded or processed at high temperatures that are sufficient to reduce pathogenic bacteria. However, the possibility for postextrusion contamination still exists. One potential method to reduce the risk of postextrusion contamination of pet foods with Salmonella is through the addition of a chemical additive coating. The objective of this research was to evaluate the ability of ß-hydroxy-ß-methylbutyrate (HMB), in either free acid (HMBFA) or calcium salt (CaHMB) form, to reduce postextrusion contamination of dry extruded dog kibble with Salmonella. Three trials were conducted with HMBFA and CaHMB coated onto the kibbles at levels of 0, 0.1, 0.3, 0.5, 0.9, and 1.5% (w/w). The coated kibbles were then inoculated with Salmonella enterica subsp. enterica Enteritidis (ATCC 13076), with enumeration done on days 0, 1, 2, 7, and 14 postinoculation. Subsamples on each day were serially diluted, spread plated to xylose lysine deoxycholate agar, and incubated at 37°C for 24 h. Salmonella colonies were then counted and log CFU per gram was calculated. The 1.5% HMBFA reduced counts by 4.9 ± 0.2 log units on day 1, whereas the positive control only decreased 2.2 ± 0.1 log units (P < 0.0001). The 1.5% CaHMB level decreased counts by 7.1 ± 0.04 log units by day 7 compared with the control decrease of 2.1 ± 0.1 log units (P < 0.0001). All HMBFA and CaHMB treatments resulted in the elimination of detectable Salmonella counts by day 14 (P < 0.0001 versus controls). In conclusion, HMB coating was effective at reducing Salmonella artificially inoculated to dog kibbles in a model of postextrusion contamination. [Mh] Termos MeSH primário: Manipulação de Alimentos/métodos Salmonella enteritidis/efeitos dos fármacos Valeratos/farmacologia [Mh] Termos MeSH secundário: Animais Contagem de Colônia Microbiana Cães Contaminação de Alimentos/prevenção & controle Microbiologia de Alimentos Seres Humanos Ácidos Pentanoicos Salmonella enteritidis/crescimento & desenvolvimento [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Pentanoic Acids); 0 (Valerates); 1BR7X184L5 (isovaleric acid); 3F752311CD (beta-hydroxyisovaleric acid) [Em] Mês de entrada: 1711 [Cu] Atualização por classe: 171102 [Lr] Data última revisão: 171102 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170601 [St] Status: MEDLINE [do] DOI: 10.4315/0362-028X.JFP-16-506

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[PMID]: 28533067 [Au] Autor: Taepucharoen K; Tarawat S; Puangcharoen M; Incharoensakdi A; Monshupanee T [Ad] Endereço: Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand. [Ti] Título: Production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) under photoautotrophy and heterotrophy by non-heterocystous N -fixing cyanobacterium. [So] Source: Bioresour Technol;239:523-527, 2017 Sep. [Is] ISSN: 1873-2976 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: The photoautotrophically grown cyanobacterium Oscillatoria okeni TISTR 8549 was found to produce bioplastic poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV). This PHBV production occurred under nitrogen deprivation (-N) that yielded PHBV accumulation of 14±4% (w/w DW) in which 3-hydroxyvalerate accounted for 5.5mol%. The heterotrophically grown (-N condition with acetate supplementation) cells under light showed no increase of PHBV storage, but under dark condition these cells increased PHBV accumulation to 42±8% (w/w DW) with 6.5mol% of 3-hydroxyvalerate. Compared to poly-3-hydroxybutyrate (PHB), the PHBV from O. okeni had a lower melting temperature by 5-7°C, a higher % elongation at break by 4-7times and a greater Young's elastic modulus by 2.3-2.5times. [Mh] Termos MeSH primário: Cianobactérias Hidroxibutiratos [Mh] Termos MeSH secundário: Ácido 3-Hidroxibutírico Processos Heterotróficos Ácidos Pentanoicos Poliésteres [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Hydroxybutyrates); 0 (Pentanoic Acids); 0 (Polyesters); 10237-77-1 (beta-hydroxyvaleric acid); 26063-00-3 (poly-beta-hydroxybutyrate); TZP1275679 (3-Hydroxybutyric Acid) [Em] Mês de entrada: 1711 [Cu] Atualização por classe: 171109 [Lr] Data última revisão: 171109 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170524 [St] Status: MEDLINE

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[PMID]: 28532241 [Au] Autor: Kihara T; Hiroe A; Ishii-Hyakutake M; Mizuno K; Tsuge T [Ad] Endereço: a Department of Materials Science and Engineering, Tokyo Institute of Technology , Midori-ku, Yokohama , Japan. [Ti] Título: Bacillus cereus-type polyhydroxyalkanoate biosynthetic gene cluster contains R-specific enoyl-CoA hydratase gene. [So] Source: Biosci Biotechnol Biochem;81(8):1627-1635, 2017 Aug. [Is] ISSN: 1347-6947 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: Bacillus cereus and Bacillus megaterium both accumulate polyhydroxyalkanoate (PHA) but their PHA biosynthetic gene (pha) clusters that code for proteins involved in PHA biosynthesis are different. Namely, a gene encoding MaoC-like protein exists in the B. cereus-type pha cluster but not in the B. megaterium-type pha cluster. MaoC-like protein has an R-specific enoyl-CoA hydratase (R-hydratase) activity and is referred to as PhaJ when involved in PHA metabolism. In this study, the pha cluster of B. cereus YB-4 was characterized in terms of PhaJ's function. In an in vitro assay, PhaJ from B. cereus YB-4 (PhaJ ) exhibited hydration activity toward crotonyl-CoA. In an in vivo assay using Escherichia coli as a host for PHA accumulation, the recombinant strain expressing PhaJ and PHA synthase led to increased PHA accumulation, suggesting that PhaJ functioned as a monomer supplier. The monomer composition of the accumulated PHA reflected the substrate specificity of PhaJ , which appeared to prefer short chain-length substrates. The pha cluster from B. cereus YB-4 functioned to accumulate PHA in E. coli; however, it did not function when the phaJ gene was deleted. The B. cereus-type pha cluster represents a new example of a pha cluster that contains the gene encoding PhaJ. [Mh] Termos MeSH primário: Família Multigênica [Mh] Termos MeSH secundário: Acil Coenzima A/metabolismo Aciltransferases/genética Aciltransferases/metabolismo Bacillus cereus/enzimologia Bacillus cereus/genética Bacillus megaterium/enzimologia Bacillus megaterium/genética Proteínas de Bactérias/genética Proteínas de Bactérias/metabolismo Ácido Butírico/metabolismo Caproatos/metabolismo Clonagem Molecular Enoil-CoA Hidratase/genética Enoil-CoA Hidratase/metabolismo Escherichia coli/genética Escherichia coli/metabolismo Expressão Gênica Glucose/metabolismo Ácidos Pentanoicos/metabolismo Plasmídeos/química Plasmídeos/metabolismo Poli-Hidroxialcanoatos/biossíntese Proteínas Recombinantes Especificidade da Espécie Especificidade por Substrato [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Acyl Coenzyme A); 0 (Bacterial Proteins); 0 (Caproates); 0 (Pentanoic Acids); 0 (Polyhydroxyalkanoates); 0 (Recombinant Proteins); 107-92-6 (Butyric Acid); 1F8SN134MX (hexanoic acid); 992-67-6 (crotonyl-coenzyme A); EC 2.3.- (Acyltransferases); EC 2.3.1.- (poly(3-hydroxyalkanoic acid) synthase); EC 4.2.1.17 (Enoyl-CoA Hydratase); GZK92PJM7B (n-pentanoic acid); IY9XDZ35W2 (Glucose) [Em] Mês de entrada: 1707 [Cu] Atualização por classe: 170726 [Lr] Data última revisão: 170726 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170524 [St] Status: MEDLINE [do] DOI: 10.1080/09168451.2017.1325314

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[PMID]: 28412665 [Au] Autor: Chi J; Ling Y; Jenkins R; Li F [Ad] Endereço: PPD Inc., Middleton, WI, United States. [Ti] Título: Quantitation of levodopa and carbidopa in rat plasma by LC-MS/MS: The key role of ion-pairing reversed-phase chromatography. [So] Source: J Chromatogr B Analyt Technol Biomed Life Sci;1054:1-9, 2017 Jun 01. [Is] ISSN: 1873-376X [Cp] País de publicação: Netherlands [La] Idioma: eng [Ab] Resumo: A simple and selective bioanalytical method was developed for simultaneous determination of levodopa and carbidopa in rat plasma by LC-MS/MS. Levodopa and carbidopa are small polar molecules, posing challenges in the development of selective and efficient chromatography conditions. Perfluoropentanoic acid (PFPA), a volatile ion-pairing agent, was utilized to enhance chromatographic characteristics of both compounds in the reversed-phase mechanism. The ion-pairing chromatography played an essential role in mitigating matrix effects and achieving adequate separation between interfering background peaks and those of the analytes of interest, especially for levodopa. A 96-well based, automated liquid-liquid extraction, via the use Hamilton NIMBUS liquid handlers, was developed. Butyl alcohol, when mixed with ethyl acetate, greatly increased the recovery of both levodopa and carbidopa. The addition of PFPA further enhanced recovery for both analytes. Sodium metabisulfite, an antioxidant, was used to stabilize levodopa and carbidopa in rat plasma. The method was validated in the ranges of 50-10,000ng/mL and 25-5000ng/mL for levodopa and carbidopa, respectively, using levodopa-d3 and carbidopa-d3 as internal standards. The validated method was successfully applied to analyze rat plasma samples from in-life studies. [Mh] Termos MeSH primário: Carbidopa/sangue Cromatografia de Fase Reversa/métodos Dopaminérgicos/sangue Levodopa/sangue Espectrometria de Massas em Tandem/métodos [Mh] Termos MeSH secundário: Animais Carbidopa/análise Dopaminérgicos/análise Levodopa/análise Limite de Detecção Extração Líquido-Líquido/métodos Ácidos Pentanoicos/química Ratos [Pt] Tipo de publicação: JOURNAL ARTICLE; VALIDATION STUDIES [Nm] Nome de substância: 0 (Dopamine Agents); 0 (Pentanoic Acids); 0 (perfluoropentanoic acid); 46627O600J (Levodopa); MNX7R8C5VO (Carbidopa) [Em] Mês de entrada: 1706 [Cu] Atualização por classe: 170606 [Lr] Data última revisão: 170606 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170417 [St] Status: MEDLINE

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