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[PMID]:29277370
[Au] Autor:Chand S; Ray S; Wanigasekara E; Yadav P; Crawford JA; Armstrong DW; Rajeshwar K; Pierce BS
[Ad] Endereço:Department of Chemistry and Biochemistry, The University of Texas at Arlington, Texas 76019, USA; Department of Biology, Catholic University of America, Washington DC 20064, USA.
[Ti] Título:Improved rate of substrate oxidation catalyzed by genetically-engineered myoglobin.
[So] Source:Arch Biochem Biophys;639:44-51, 2018 02 01.
[Is] ISSN:1096-0384
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:This study showcases the potential of unnatural amino acids to enable non-natural functions when incorporated in the protein scaffold of heme metalloproteins. For this purpose, a genetically-engineered myoglobin (Mb) mutant was created by incorporating redox-active 3-amino-l-tyrosine (NH Tyr) into its active site, replacing the distal histidine (H64) with NH Tyr. In peroxide-shunt assays, this variant exhibits an increased rate of turnover for thioanisole and benzaldehyde oxidation as compared to the wild-type (WT) Mb. Indeed, in the presence of excess hydrogen peroxide (H O ), a 9-fold and 81-fold increase in activity was observed over multiple turnovers for thioanisole sulfoxidation and benzoic acid formation, respectively. The increased oxidation activity in the H64NH Tyr Mb mutant underlined the role of NH Tyr in the distal active-site scaffold in peroxide activation. Kinetic, electrochemical, and EPR spectroscopic experiments were performed. On the basis of these studies, it is argued that the single NH Tyr residue within the Mb variant simultaneously serves the role of the conserved His/Arg-pair within the distal pocket of horseradish peroxidase.
[Mh] Termos MeSH primário: Substituição de Aminoácidos
Ácido Benzoico/química
Mioglobina/química
Sulfetos/química
[Mh] Termos MeSH secundário: Animais
Mutação de Sentido Incorreto
Mioglobina/genética
Oxirredução
Cachalote
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Myoglobin); 0 (Sulfides); 8SKN0B0MIM (Benzoic Acid); BB4K737YF4 (methylphenylsulfide)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180214
[Lr] Data última revisão:
180214
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171227
[St] Status:MEDLINE


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[PMID]:28946287
[Au] Autor:Fujiyoshi T; Ikami T; Kikukawa K; Kobayashi M; Takai R; Kozaki D; Yamamoto A
[Ad] Endereço:Food Analysis Technology Center SUNATEC, 9-5 Akahorishinmachi, Yokkaichi, Mie 510-0825, Japan; College of Bioscience and Biotechnology, Chubu University, 1200 Matsumoto-cho, Kasugai, Aichi 487-8501, Japan. Electronic address: tfujiyoshi@mac.or.jp.
[Ti] Título:Direct quantitation of the preservatives benzoic and sorbic acid in processed foods using derivative spectrophotometry combined with micro dialysis.
[So] Source:Food Chem;240:386-390, 2018 Feb 01.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The preservatives benzoic acid and sorbic acid are generally quantified with separation techniques, such as HPLC or GC. Here we describe a new method for determining these compounds in processed food samples based on a narrowness of the UV-visible spectral band width with derivative processing. It permits more selective identification and determination of target analytes in matrices. After a sample is purified by micro dialysis, UV spectra of sample solutions were measured and fourth order derivatives of the spectrum were calculated. The amplitude between the maximum and minimum values in a high-order derivative spectrum was used for the determination of benzoic acid and sorbic acid. Benzoic acid and sorbic acid levels in several commercially available processed foods were measured by HPLC and the proposed spectrometry method. The levels obtained by the two methods were highly correlated (r >0.97) for both preservatives.
[Mh] Termos MeSH primário: Ácido Benzoico/análise
Ácido Sórbico/análise
[Mh] Termos MeSH secundário: Conservantes de Alimentos
Conservantes Farmacêuticos
Diálise Renal
Espectrofotometria
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Food Preservatives); 0 (Preservatives, Pharmaceutical); 8SKN0B0MIM (Benzoic Acid); X045WJ989B (Sorbic Acid)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170927
[St] Status:MEDLINE


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[PMID]:29031450
[Au] Autor:Li H; Cao L; Yang C; Zhang Z; Zhang B; Deng K
[Ad] Endereço:Key Laboratory of Catalysis and Material Sciences of the State Ethnic Affairs Commission & Ministry of Education, College of Chemistry and Material Science, South-Central University For Nationalities, Wuhan 430074, China.
[Ti] Título:Selective oxidation of benzyl alcohols to benzoic acid catalyzed by eco-friendly cobalt thioporphyrazine catalyst supported on silica-coated magnetic nanospheres.
[So] Source:J Environ Sci (China);60:84-90, 2017 Oct.
[Is] ISSN:1001-0742
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A novel magnetically recoverable thioporphyrazine catalyst (CoPz(S-Bu) /SiO @Fe O ) was prepared by immobilization of the cobalt octkis(butylthio) porphyrazine complex (CoPz(S-Bu) ) on silica-coated magnetic nanospheres (SiO @Fe O ). The composite CoPz(S-Bu) /SiO @Fe O appeared to be an active catalyst in the oxidation of benzyl alcohol in aqueous solution using hydrogen peroxide (H O ) as oxidant under Xe-lamp irradiation, with 36.4% conversion of benzyl alcohol, about 99% selectivity for benzoic acid and turnover number (TON) of 61.7 at ambient temperature. The biomimetic catalyst CoPz(S-Bu) was supported on the magnetic carrier SiO @Fe O so as to suspend it in aqueous solution to react with substrates, utilizing its lipophilicity. Meanwhile the CoPz(S-Bu) can use its unique advantages to control the selectivity of photocatalytic oxidation without the substrate being subjected to deep oxidation. The influence of various reaction parameters on the conversion rate of benzyl alcohol and selectivity of benzoic acid was investigated in detail. Moreover, photocatalytic oxidation of substituted benzyl alcohols was obtained with high conversion and excellent selectivity, specifically conversion close to 70%, selectivity close to 100% and TON of 113.6 for para-position electron-donating groups. The selectivity and eco-friendliness of the biomimetic photocatalyst give it great potential for practical applications.
[Mh] Termos MeSH primário: Ácido Benzoico/química
Álcoois Benzílicos/química
Modelos Químicos
Nanosferas/química
[Mh] Termos MeSH secundário: Catálise
Peróxido de Hidrogênio
Magnetismo
Oxirredução
Dióxido de Silício
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Benzyl Alcohols); 7631-86-9 (Silicon Dioxide); 8SKN0B0MIM (Benzoic Acid); BBX060AN9V (Hydrogen Peroxide)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171019
[Lr] Data última revisão:
171019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171017
[St] Status:MEDLINE


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[PMID]:28625491
[Au] Autor:Jahan H; Choudhary MI; Manzoor M; Khan KM; Perveen S; Atta-Ur-Rahman
[Ad] Endereço:Dr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences, University of Karachi, Karachi, 75270, Pakistan. Electronic address: jahan_pcmd@yahoo.com.
[Ti] Título:Insulinotropic action of 2, 4-dinitroanilino-benzoic acid through the attenuation of pancreatic beta-cell lesions in diabetic rats.
[So] Source:Chem Biol Interact;273:237-244, 2017 Aug 01.
[Is] ISSN:1872-7786
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:Beta cell destruction plays a key role in the pathogenesis of type 1 diabetes mellitus. It has also been argued that beta-cell mass is compromised in some cases of type 2 diabetes, although this is still debated. Currently, the failure of oral antidiabetic insulin secretagogue drugs to properly manage type 2 diabetes demands novel approaches for the treatment of this condition. The aim of the present study was to investigate the in vitro and in vivo antidiabetic effect in STZ-induced diabetic rats, and maximum tolerated dose (MTD) safety of novel anthranilic acid derivative, 2, 4-dinitroanilino-benzoic acid (1). Anthranilic acid derivative 1 was also evaluated for insulinotropic action on STZ-mediated pancreatic beta-cell lesions in diabetic rats. During an eight week study, oral glucose tolerance test, fasting blood glucose, and serum insulin levels, and pancreatic insulin contents were measured in four different groups of Wistar rats; control, STZ-induced diabetic, gliclazide-treated, and anthranilic acid derivative-treated diabetic rats. Beta-cell number and islet area were also quantified, and immunohistochemical study was performed. In vitro studies in cells showed that 2, 4-dinitroanilino-benzoic acid (1) did not adversely effect the cells viability. We found that the derivative 1 significantly improved the glucose tolerance, fasting blood glucose, and HbA1c levels, serum insulin levels, and pancreatic insulin contents (P < 0.05), comparable to gliclazide-treated group. The derivative 1 exhibited a significant insulinotropic action on diabetic pancreas, and caused an increased immunoreactivity for insulin, as compared to gliclazide-treated group. Together these results suggest that treatment of diabetic rats with 2, 4-dinitroanilino-benzoic acid (1) improved the glucose tolerance, fasting blood glucose, and HbA1c levels most probably by restoring the functional activities of the pancreas via its insulinotropic action. This indicates that the derivative 1 can serve as lead for the treatment of diabetes caused by low insulin levels.
[Mh] Termos MeSH primário: Compostos de Anilina/farmacologia
Ácido Benzoico/farmacologia
Diabetes Mellitus Experimental/tratamento farmacológico
Diabetes Mellitus Tipo 2/tratamento farmacológico
Hipoglicemiantes/farmacologia
Células Secretoras de Insulina/efeitos dos fármacos
[Mh] Termos MeSH secundário: Compostos de Anilina/administração & dosagem
Compostos de Anilina/química
Animais
Ácido Benzoico/administração & dosagem
Ácido Benzoico/química
Sobrevivência Celular/efeitos dos fármacos
Células Cultivadas
Diabetes Mellitus Experimental/induzido quimicamente
Diabetes Mellitus Experimental/patologia
Diabetes Mellitus Tipo 2/induzido quimicamente
Diabetes Mellitus Tipo 2/patologia
Relação Dose-Resposta a Droga
Teste de Tolerância a Glucose
Seres Humanos
Hipoglicemiantes/administração & dosagem
Hipoglicemiantes/química
Insulina/metabolismo
Células Secretoras de Insulina/patologia
Ratos
Estreptozocina
Relação Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Aniline Compounds); 0 (Hypoglycemic Agents); 0 (Insulin); 5BI780R6W6 (2,4-dinitroaniline); 5W494URQ81 (Streptozocin); 8SKN0B0MIM (Benzoic Acid)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171002
[Lr] Data última revisão:
171002
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170620
[St] Status:MEDLINE


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[PMID]:28615449
[Au] Autor:Hoben JP; Lubner CE; Ratzloff MW; Schut GJ; Nguyen DMN; Hempel KW; Adams MWW; King PW; Miller AF
[Ad] Endereço:From the Department of Chemistry, University of Kentucky, Lexington, Kentucky 40506.
[Ti] Título:Equilibrium and ultrafast kinetic studies manipulating electron transfer: A short-lived flavin semiquinone is not sufficient for electron bifurcation.
[So] Source:J Biol Chem;292(34):14039-14049, 2017 Aug 25.
[Is] ISSN:1083-351X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Flavin-based electron transfer bifurcation is emerging as a fundamental and powerful mechanism for conservation and deployment of electrochemical energy in enzymatic systems. In this process, a pair of electrons is acquired at intermediate reduction potential ( intermediate reducing power), and each electron is passed to a different acceptor, one with lower and the other with higher reducing power, leading to "bifurcation." It is believed that a strongly reducing semiquinone species is essential for this process, and it is expected that this species should be kinetically short-lived. We now demonstrate that the presence of a short-lived anionic flavin semiquinone (ASQ) is not sufficient to infer the existence of bifurcating activity, although such a species may be necessary for the process. We have used transient absorption spectroscopy to compare the rates and mechanisms of decay of ASQ generated photochemically in bifurcating NADH-dependent ferredoxin-NADP oxidoreductase and the non-bifurcating flavoproteins nitroreductase, NADH oxidase, and flavodoxin. We found that different mechanisms dominate ASQ decay in the different protein environments, producing lifetimes ranging over 2 orders of magnitude. Capacity for electron transfer among redox cofactors charge recombination with nearby donors can explain the range of ASQ lifetimes that we observe. Our results support a model wherein efficient electron propagation can explain the short lifetime of the ASQ of bifurcating NADH-dependent ferredoxin-NADP oxidoreductase I and can be an indication of capacity for electron bifurcation.
[Mh] Termos MeSH primário: Proteínas de Bactérias/metabolismo
Flavina-Adenina Dinucleotídeo/análogos & derivados
Flavodoxina/metabolismo
Modelos Moleculares
Complexos Multienzimáticos/metabolismo
NADH NADPH Oxirredutases/metabolismo
Nitrorredutases/metabolismo
Oxirredutases/metabolismo
[Mh] Termos MeSH secundário: Apoenzimas/química
Apoenzimas/genética
Apoenzimas/metabolismo
Proteínas de Bactérias/química
Proteínas de Bactérias/genética
Ácido Benzoico/química
Ácido Benzoico/metabolismo
Biocatálise
Desulfovibrio vulgaris/enzimologia
Transporte de Elétrons
Enterobacter cloacae/enzimologia
Flavina-Adenina Dinucleotídeo/química
Flavina-Adenina Dinucleotídeo/metabolismo
Flavodoxina/química
Flavodoxina/genética
Holoenzimas/química
Holoenzimas/genética
Holoenzimas/metabolismo
Complexos Multienzimáticos/química
Complexos Multienzimáticos/genética
NADH NADPH Oxirredutases/química
NADH NADPH Oxirredutases/genética
Nitrorredutases/química
Nitrorredutases/genética
Oxirredução
Oxirredutases/química
Oxirredutases/genética
Pyrococcus furiosus/enzimologia
Proteínas Recombinantes de Fusão/química
Proteínas Recombinantes de Fusão/metabolismo
Proteínas Recombinantes/química
Proteínas Recombinantes/metabolismo
Mutação Silenciosa
Thermus thermophilus/enzimologia
ortoaminobenzoatos/química
ortoaminobenzoatos/metabolismo
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Apoenzymes); 0 (Bacterial Proteins); 0 (Flavodoxin); 0 (Holoenzymes); 0 (Multienzyme Complexes); 0 (Recombinant Fusion Proteins); 0 (Recombinant Proteins); 0 (ortho-Aminobenzoates); 0YS975XI6W (anthranilic acid); 146-14-5 (Flavin-Adenine Dinucleotide); 35919-91-6 (flavin semiquinone); 8SKN0B0MIM (Benzoic Acid); 952VN06WBB (fenamic acid); EC 1.- (Oxidoreductases); EC 1.18.1.3 (ferredoxin-NAD+ reductase); EC 1.6.- (NADH oxidase); EC 1.6.- (NADH, NADPH Oxidoreductases); EC 1.7.- (Nitroreductases)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170912
[Lr] Data última revisão:
170912
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170616
[St] Status:MEDLINE
[do] DOI:10.1074/jbc.M117.794214


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[PMID]:28478294
[Au] Autor:Zrinyi N; Pham AL
[Ad] Endereço:Department of Civil and Environmental Engineering, Carleton University, Ottawa, ON K1S 5B6, Canada.
[Ti] Título:Oxidation of benzoic acid by heat-activated persulfate: Effect of temperature on transformation pathway and product distribution.
[So] Source:Water Res;120:43-51, 2017 Sep 01.
[Is] ISSN:1879-2448
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Heat activates persulfate (S O ) into sulfate radical (SO ), a powerful oxidant capable of transforming a wide variety of contaminants. Previous studies have shown that an increase in temperature accelerates the rates of persulfate activation and contaminant transformation. However, few studies have considered the effect of temperature on contaminant transformation pathway. The objective of this study was to determine how temperature (T = 22-70 °C) influences the activation of persulfate, the transformation of benzoic acid (i.e., a model compound), and the distribution of benzoic acid oxidation products. The time-concentration profiles of the products suggest that benzoic acid was transformed via decarboxylation and hydroxylation mechanisms, with the former becoming increasingly important at elevated temperatures. The pathway through which the products were further oxidized was also influenced by the temperature of persulfate activation. Our findings suggest that the role of temperature in the persulfate-based treatment systems is not limited only to controlling the rates of sulfate and hydroxyl radical generation. The ability of sulfate radical to initiate decarboxylation reactions and, more broadly, fragmentation reactions, as well as the effect of temperature on these transformation pathways could be important to the transformation of a number of organic contaminants.
[Mh] Termos MeSH primário: Ácido Benzoico
Temperatura Ambiente
[Mh] Termos MeSH secundário: Temperatura Alta
Oxirredução
Sulfatos
Poluentes Químicos da Água
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Sulfates); 0 (Water Pollutants, Chemical); 8SKN0B0MIM (Benzoic Acid)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171013
[Lr] Data última revisão:
171013
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170508
[St] Status:MEDLINE


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[PMID]:28364832
[Au] Autor:Lynch H; Leonard FC; Walia K; Lawlor PG; Duffy G; Fanning S; Markey BK; Brady C; Gardiner GE; Argüello H
[Ad] Endereço:National Food Research Centre, Teagasc, Ashtown, Dublin, Ireland; UCD Veterinary Sciences Centre, University College Dublin, Dublin, Ireland. Electronic address: Lynchhelen28@gmail.com.
[Ti] Título:Investigation of in-feed organic acids as a low cost strategy to combat Salmonella in grower pigs.
[So] Source:Prev Vet Med;139(Pt A):50-57, 2017 Apr 01.
[Is] ISSN:1873-1716
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Salmonella carriage in pigs is a significant food safety issue. Dietary supplementation with organic acids has previously been shown to reduce shedding and transmission of Salmonella. Therefore, this study aimed to examine the effect of three commercially available organic acid-based products on Salmonella levels in grower pigs, using a model of experimental infection that closely mimics natural exposure to the organism. Seven week old trial pigs (n=40) with a mean weight of 14.7kg were placed in one of four pens with 10 pigs/pen. Pens had previously been contaminated with Salmonella Typhimurium 4,[5],12;i;- via seeder pigs. Trial pigs received one of four diets for 28days: 1, control diet; 2, sodium butyrate supplemented diet; 3, benzoic acid supplemented diet and 4, formic-citric acid supplemented diet. A further 10 pigs were placed in a Salmonella-free pen receiving the control diet. Pigs were weighed and blood sampled on days 0 and 28. Faeces was collected on day 0, 2, 3, 5, 7, 14, 21 and 28 and examined for Salmonella. On day 28, 5 pigs/group were euthanised and ileocaecal lymph nodes (ILN) and caecal contents sampled for culture. The remaining 5 pigs/pen were then fed the control diet and faeces were collected on days 35 and 42. On day 42 pigs were euthanised and ILN and caecal contents tested for Salmonella levels. The trial was repeated once. Within the first two days of exposure to the contaminated environment, 96% (77/80) of pigs became infected. Most pigs shed Salmonella at levels of between 10 -10 CFU/g faeces for at least 7days post-exposure. A significant reduction in Salmonella faecal concentration was observed after supplementation with sodium butyrate (p=0.001) and a formic citric acid blend (p<0.0001). Average daily weight gain (ADWG) was significantly increased in all groups fed the supplemented feed when compared to the positive control group. The use of sodium butyrate or a blend of formic and citric acid in feed could be considered a cost-effective control measure to reduce Salmonella faecal shedding and improve ADWG in Salmonella infected herds.
[Mh] Termos MeSH primário: Ração Animal
Ácido Butírico/administração & dosagem
Ácido Cítrico/administração & dosagem
Formiatos/administração & dosagem
Salmonelose Animal/prevenção & controle
Doenças dos Suínos/prevenção & controle
[Mh] Termos MeSH secundário: Análise de Variância
Animais
Derrame de Bactérias/efeitos dos fármacos
Ácido Benzoico/administração & dosagem
Ceco/microbiologia
Suplementos Nutricionais
Eutanásia Animal
Fezes/microbiologia
Distribuição Aleatória
Salmonelose Animal/sangue
Salmonella typhimurium/isolamento & purificação
Suínos
Doenças dos Suínos/sangue
Doenças dos Suínos/microbiologia
Ganho de Peso
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Formates); 0YIW783RG1 (formic acid); 107-92-6 (Butyric Acid); 2968PHW8QP (Citric Acid); 8SKN0B0MIM (Benzoic Acid)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170821
[Lr] Data última revisão:
170821
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170403
[St] Status:MEDLINE


  8 / 1781 MEDLINE  
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[PMID]:28320616
[Au] Autor:Geldenhuys WJ; Bergeron SA; Mullins JE; Aljammal R; Gaasch BL; Chen WC; Yun J; Hazlehurst LA
[Ad] Endereço:Department of Pharmaceutical Sciences, School of Pharmacy, West Virginia University, Morgantown, WV 26506, United States. Electronic address: werner.geldenhuys@hsc.wvu.edu.
[Ti] Título:High-content screen using zebrafish (Danio rerio) embryos identifies a novel kinase activator and inhibitor.
[So] Source:Bioorg Med Chem Lett;27(9):2029-2037, 2017 05 01.
[Is] ISSN:1464-3405
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In this report we utilized zebrafish (Danio rerio) embryos in a phenotypical high-content screen (HCS) to identify novel leads in a cancer drug discovery program. We initially validated our HCS model using the flavin adenosine dinucleotide (FAD) containing endoplasmic reticulum (ER) enzyme, endoplasmic reticulum oxidoreductase (ERO1) inhibitor EN460. EN460 showed a dose response effect on the embryos with a dose of 10µM being significantly lethal during early embryonic development. The HCS campaign which employed a small library identified a promising lead compound, a naphthyl-benzoic acid derivative coined compound 1 which had significant dosage and temporally dependent effects on notochord and muscle development in zebrafish embryos. Screening a 369 kinase member panel we show that compound 1 is a PIM3 kinase inhibitor (IC =4.078µM) and surprisingly a DAPK1 kinase agonist/activator (EC =39.525µM). To our knowledge this is the first example of a small molecule activating DAPK1 kinase. We provide a putative model for increased phosphate transfer in the ATP binding domain when compound 1 is virtually docked with DAPK1. Our data indicate that observable phenotypical changes can be used in future zebrafish screens to identify compounds acting via similar molecular signaling pathways.
[Mh] Termos MeSH primário: Descoberta de Drogas/métodos
Embrião não Mamífero/efeitos dos fármacos
Ativadores de Enzimas/química
Ativadores de Enzimas/farmacologia
Inibidores de Proteínas Quinases/química
Inibidores de Proteínas Quinases/farmacologia
Peixe-Zebra/embriologia
[Mh] Termos MeSH secundário: Animais
Antineoplásicos/química
Antineoplásicos/farmacologia
Ácido Benzoico/química
Ácido Benzoico/farmacologia
Proteínas Quinases Associadas com Morte Celular/metabolismo
Ensaios de Seleção de Medicamentos Antitumorais/métodos
Embrião não Mamífero/enzimologia
Ativação Enzimática/efeitos dos fármacos
Neoplasias/tratamento farmacológico
Neoplasias/enzimologia
Proteínas Serina-Treonina Quinases/antagonistas & inibidores
Proteínas Serina-Treonina Quinases/metabolismo
Proteínas de Peixe-Zebra/antagonistas & inibidores
Proteínas de Peixe-Zebra/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (Enzyme Activators); 0 (Protein Kinase Inhibitors); 0 (Zebrafish Proteins); 8SKN0B0MIM (Benzoic Acid); EC 2.7.11.1 (Death-Associated Protein Kinases); EC 2.7.11.1 (Protein-Serine-Threonine Kinases)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171124
[Lr] Data última revisão:
171124
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170322
[St] Status:MEDLINE


  9 / 1781 MEDLINE  
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[PMID]:28317083
[Au] Autor:Ghadiri M; Shirazian S
[Ad] Endereço:Young Researchers and Elite Club, South Tehran Branch, Islamic Azad University, Tehran, Iran.
[Ti] Título:Numerical simulation of reactive extraction of benzoic acid from wastewater via membrane contactors.
[So] Source:Environ Sci Pollut Res Int;24(12):11518-11527, 2017 Apr.
[Is] ISSN:1614-7499
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Membrane-based non-dispersive solvent extraction is used in many chemical processes due to its significant benefits such as straightforward scale-up and low energy consumption. A mechanistic model was developed to predict recovery of benzoic acid (BA) from wastewater using membrane contactors. Model equations were derived for benzoic acid transport in the membrane module, and solved using FEM. The model findings were compared with experimental results, and an average deviation of 4% was observed between experimental and simulation results. Simulations showed that change in organic phase flowrate and initial concentration of BA does not have considerable effect on the removal efficiency of benzoic acid. In addition, increasing feed flowrate leads to the enhancement of convective mass transfer flux in the tube side of membrane contactor which decreases removal efficiency of benzoic acid.
[Mh] Termos MeSH primário: Ácido Benzoico/análise
Águas Residuais
[Mh] Termos MeSH secundário: Membranas Artificiais
Modelos Teóricos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Membranes, Artificial); 0 (Waste Water); 8SKN0B0MIM (Benzoic Acid)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171103
[Lr] Data última revisão:
171103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170321
[St] Status:MEDLINE
[do] DOI:10.1007/s11356-017-8817-8


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[PMID]:28270109
[Au] Autor:Silva VO; Pereira LJ; Murata RM
[Ad] Endereço:Herman Ostrow School of Dentistry, Division of Periodontology Diagnostic Sciences, Dental Hygiene & Biomedical Science, University of Southern California, Los Angeles, CA, USA.
[Ti] Título:Oral microbe-host interactions: influence of ß-glucans on gene expression of inflammatory cytokines and metabolome profile.
[So] Source:BMC Microbiol;17(1):53, 2017 Mar 07.
[Is] ISSN:1471-2180
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The aim of this study was to evaluate the effects of ß-glucan on the expression of inflammatory mediators and metabolomic profile of oral cells [keratinocytes (OBA-9) and fibroblasts (HGF-1) in a dual-chamber model] infected by Aggregatibacter actinomycetemcomitans. The periodontopathogen was applied and allowed to cross the top layer of cells (OBA-9) to reach the bottom layer of cells (HGF-1) and induce the synthesis of immune factors and cytokines in the host cells. ß-glucan (10 µg/mL or 20 µg/mL) were added, and the transcriptional factors and metabolites produced were quantified in the remaining cell layers and supernatant. RESULTS: The relative expression of interleukin (IL)-1-α and IL-18 genes in HGF-1 decreased with 10 µg/mL or 20 µg/mL of ß-glucan, where as the expression of PTGS-2 decreased only with 10 µg/mL. The expression of IL-1-α increased with 20 µg/mL and that of IL-18 increased with 10 µg/mL in OBA-9; the expression of BCL 2, EP 300, and PTGS-2 decreased with the higher dose of ß-glucan. The production of the metabolite 4-aminobutyric acid presented lower concentrations under 20 µg/mL, whereas the concentrations of 2-deoxytetronic acid NIST and oxalic acid decreased at both concentrations used. Acetophenone, benzoic acid, and pinitol presented reduced concentrations only when treated with 10 µg/mL of ß-glucan. CONCLUSIONS: Treatment with ß-glucans positively modulated the immune response and production of metabolites.
[Mh] Termos MeSH primário: Aggregatibacter actinomycetemcomitans/efeitos dos fármacos
Aggregatibacter actinomycetemcomitans/fisiologia
Citocinas/efeitos dos fármacos
Expressão Gênica/efeitos dos fármacos
Interações Hospedeiro-Patógeno
Metaboloma/efeitos dos fármacos
beta-Glucanas/farmacologia
[Mh] Termos MeSH secundário: Acetofenonas/metabolismo
Anti-Infecciosos/farmacologia
Ácido Benzoico/metabolismo
Técnicas de Cultura de Células/métodos
Linhagem Celular
Técnicas de Cocultura
Ciclo-Oxigenase 2/metabolismo
Citocinas/genética
Citocinas/imunologia
Proteína p300 Associada a E1A/metabolismo
Fibroblastos/efeitos dos fármacos
Fibroblastos/metabolismo
Interações Hospedeiro-Patógeno/imunologia
Seres Humanos
Hidroxibutiratos/metabolismo
Imunomodulação
Inositol/análogos & derivados
Inositol/metabolismo
Interleucina-18/genética
Interleucina-1alfa/genética
Queratinócitos/efeitos dos fármacos
Queratinócitos/metabolismo
Linfoma de Células B/metabolismo
Metaboloma/genética
Metaboloma/imunologia
Boca/imunologia
Boca/microbiologia
Ácido Oxálico/metabolismo
Doenças Periodontais/imunologia
Doenças Periodontais/microbiologia
Proteínas Proto-Oncogênicas c-bcl-2/metabolismo
Reação em Cadeia da Polimerase em Tempo Real
beta-Glucanas/administração & dosagem
beta-Glucanas/metabolismo
Ácido gama-Aminobutírico/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acetophenones); 0 (Anti-Infective Agents); 0 (BCL2 protein, human); 0 (Cytokines); 0 (Hydroxybutyrates); 0 (Interleukin-18); 0 (Interleukin-1alpha); 0 (Proto-Oncogene Proteins c-bcl-2); 0 (beta-Glucans); 1518-61-2 (3,4-dihydroxybutanoic acid); 484-68-4 (pinitol); 4L6452S749 (Inositol); 56-12-2 (gamma-Aminobutyric Acid); 8SKN0B0MIM (Benzoic Acid); 9E7R5L6H31 (Oxalic Acid); EC 1.14.99.1 (Cyclooxygenase 2); EC 1.14.99.1 (PTGS2 protein, human); EC 2.3.1.48 (E1A-Associated p300 Protein); EC 2.3.1.48 (EP300 protein, human); RK493WHV10 (acetophenone)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170925
[Lr] Data última revisão:
170925
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170309
[St] Status:MEDLINE
[do] DOI:10.1186/s12866-017-0946-1



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