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[PMID]:28825823
[Au] Autor:Taylor M; Bickel A; Mannion R; Bell E; Harrigan GG
[Ad] Endereço:Monsanto Company , 800 North Lindbergh Boulevard, St. Louis, Missouri 63167, United States.
[Ti] Título:Dicamba-Tolerant Soybeans (Glycine max L.) MON 87708 and MON 87708 × MON 89788 Are Compositionally Equivalent to Conventional Soybean.
[So] Source:J Agric Food Chem;65(36):8037-8045, 2017 Sep 13.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Herbicide-tolerant crops can expand both tools for and timing of weed control strategies. MON 87708 soybean has been developed through genetic modification and confers tolerance to the dicamba herbicide. As part of the safety assessment conducted for new genetically modified (GM) crop varieties, a compositional assessment of MON 87708 was performed. Levels of key soybean nutrients and anti-nutrients in harvested MON 87708 were compared to levels of those components in a closely related non-GM variety as well as to levels measured in other conventional soybean varieties. From this analysis, MON 87708 was shown to be compositionally equivalent to its comparator. A similar analysis conducted for a stacked trait product produced by conventional breeding, MON 87708 × MON 89788, which confers tolerance to both dicamba and glyphosate herbicides, reached the same conclusion. These results are consistent with other results that demonstrate no compositional impact of genetic modification, except in those cases where an impact was an intended outcome.
[Mh] Termos MeSH primário: Dicamba/farmacologia
Resistência a Herbicidas
Herbicidas/farmacologia
Plantas Geneticamente Modificadas/efeitos dos fármacos
Feijão de Soja/efeitos dos fármacos
[Mh] Termos MeSH secundário: Cruzamento
Glicina/análogos & derivados
Glicina/farmacologia
Plantas Geneticamente Modificadas/genética
Feijão de Soja/genética
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Herbicides); 4632WW1X5A (glyphosate); SJG3M6RY6H (Dicamba); TE7660XO1C (Glycine)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170925
[Lr] Data última revisão:
170925
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170822
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b03844


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[PMID]:28285895
[Au] Autor:León-Ramírez CG; Cabrera-Ponce JL; Martínez-Soto D; Sánchez-Arreguin A; Aréchiga-Carvajal ET; Ruiz-Herrera J
[Ad] Endereço:Departamento de Ingeniería Genética, Unidad Irapuato, Centro de Investigación y de Estudios Avanzados del IPN, 36825 Irapuato, Guanajuato, Mexico.
[Ti] Título:Transcriptomic analysis of basidiocarp development in Ustilago maydis (DC) Cda.
[So] Source:Fungal Genet Biol;101:34-45, 2017 Apr.
[Is] ISSN:1096-0937
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Previously, we demonstrated that when Ustilago maydis (DC) Cda., a phytopathogenic basidiomycete and the causal agent of corn smut, is grown in the vicinity of maize embryogenic calli in a medium supplemented with the herbicide Dicamba, it developed gastroid-like basidiocarps. To elucidate the molecular mechanisms involved in the basidiocarp development by the fungus, we proceeded to analyze the transcriptome of the process, identifying a total of 2002 and 1064 differentially expressed genes at two developmental stages, young and mature basidiocarps, respectively. Function of these genes was analyzed with the use of different databases. MIPS analysis revealed that in the stage of young basidiocarp, among the ca. two thousand differentially expressed genes, there were some previously described for basidiocarp development in other fungal species. Additional elements that operated at this stage included, among others, genes encoding the transcription factors FOXO3, MIG3, PRO1, TEC1, copper and MFS transporters, and cytochromes P450. During mature basidiocarp development, important up-regulated genes included those encoding hydrophobins, laccases, and ferric reductase (FRE/NOX). The demonstration that a mapkk mutant was unable to form basidiocarps, indicated the importance of the MAPK signaling pathway in this developmental process.
[Mh] Termos MeSH primário: Dicamba/farmacologia
Carpóforos/genética
Transcriptoma/efeitos dos fármacos
Ustilago/genética
[Mh] Termos MeSH secundário: Carpóforos/efeitos dos fármacos
Carpóforos/crescimento & desenvolvimento
Proteínas Fúngicas/biossíntese
Perfilação da Expressão Gênica
Regulação Fúngica da Expressão Gênica/efeitos dos fármacos
Doenças das Plantas/microbiologia
Ustilago/efeitos dos fármacos
Ustilago/crescimento & desenvolvimento
Ustilago/patogenicidade
Zea mays/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fungal Proteins); SJG3M6RY6H (Dicamba)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170918
[Lr] Data última revisão:
170918
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170314
[St] Status:MEDLINE


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[PMID]:27997185
[Au] Autor:Pernak J; Giszter R; Biedziak A; Niemczak M; Olszewski R; Marcinkowska K; Praczyk T
[Ad] Endereço:Department of Chemical Technology, Poznan University of Technology , Poznan 60-965, Poland.
[Ti] Título:Alkyl(C , C , C )trimethylammonium-Based Herbicidal Ionic Liquids.
[So] Source:J Agric Food Chem;65(2):260-269, 2017 Jan 18.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In the framework of this study a synthesis methodology and characterization of long alkyl herbicidal ionic liquids (HILs) based on four commonly used herbicides (2,4-D, MCPA, MCPP, and dicamba) are presented. New HILs were obtained with high efficiency (>95%) using an acid-base reaction between herbicidal acids and hexadecyltrimethylammonium, octadecyltrimethylammonium, and behenyltrimethylammonium hydroxides in alcoholic medium. Among all synthesized salts, only three compounds comprising the MCPP anion were liquids at room temperature. Subsequently, the influence of both the alkyl chain length and the anion structure on their physicochemical properties (thermal decomposition profiles, solubility in 10 representative solvents, surface activity, density, viscosity, and refractive index) was determined. All HILs exhibited high thermal stability as well as surface activity; however, their solubility notably depended on both the length of the carbon chain and the structure of the anion. The herbicidal efficacy of the obtained salts was tested in greenhouse and field experiments. Greenhouse testing performed on common lambsquarters (Chenopodium album L.) and flixweed (Descurainia sophia L.) as test plants indicated that HILs were characterized by similar or higher efficacy compared to commercial herbicides. The results of field trials confirmed the high activity of HILs, particularly those containing phenoxyacids as anions (MCPA, 2,4-D, and MCPP).
[Mh] Termos MeSH primário: Herbicidas/química
Herbicidas/farmacologia
Líquidos Iônicos/química
[Mh] Termos MeSH secundário: Ácido 2,4-Diclorofenoxiacético/química
Ácido 2-Metil-4-clorofenoxiacético/química
Compostos de Bis-Trimetilamônio/química
Brassicaceae/efeitos dos fármacos
Técnicas de Química Sintética
Chenopodium album/efeitos dos fármacos
Dicamba/química
Herbicidas/síntese química
Líquidos Iônicos/síntese química
Líquidos Iônicos/farmacologia
Plantas Daninhas/efeitos dos fármacos
Polônia
Solubilidade
Relação Estrutura-Atividade
Propriedades de Superfície
Viscosidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bis-Trimethylammonium Compounds); 0 (Herbicides); 0 (Ionic Liquids); 2577AQ9262 (2,4-Dichlorophenoxyacetic Acid); D888C394VO (2-Methyl-4-chlorophenoxyacetic Acid); SJG3M6RY6H (Dicamba)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170502
[Lr] Data última revisão:
170502
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161221
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.6b04528


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[PMID]:27716584
[Au] Autor:Piotrowska A; Syguda A; Wyrwas B; Chrzanowski L; Heipieper HJ
[Ad] Endereço:Department of Environmental Biotechnology, Helmholtz Centre for Environmental Research-UFZ, Permoserstrasse 15, 04318 Leipzig, Germany; Faculty of Chemical Technology, Poznan University of Technology, ul. Berdychowo 4, 60-965 Poznan, Poland.
[Ti] Título:Toxicity evaluation of selected ammonium-based ionic liquid forms with MCPP and dicamba moieties on Pseudomonas putida.
[So] Source:Chemosphere;167:114-119, 2017 Jan.
[Is] ISSN:1879-1298
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Combination of the hydrophilic herbicidal anion with hydrophobic, antimicrobial ammonium cation allows to obtain compounds in ionic liquid form with better properties then conventional herbicides. Both cation and anion can be modified by selection of herbicide and the length of alkyl chains in cation structure. However the knowledge of their potential toxic effects are still limited. Furthermore, the relation between hydrophobicity associated with the length of alkyl chains and toxicity for ionic liquids has not been thoroughly studied. Therefore we investigated toxic effects of herbicidal ionic liquid forms on growth inhibition, given as EC of the common soil bacterium Pseudomonas putida. We thereby concentrated on quaternary ammonium salts. Analyzed compounds were composed of dicamba or MCPP moieties and cation with various alkyl chain lengths (n = 6,8,10) We compared them with commercial herbicides, and ammonium-based ionic liquids with neutral anion (Br ). In addition, cis-trans isomerisation of unsaturated membrane fatty acids in Pseudomonas putida was applied as the proxy for toxicity and membrane activity. We showed that toxicity increased with the length of alkyl chains. However, this correlation is only valid for six and eight carbon atom in alkyl chains, where for n = 10 the EC values rise by one order of magnitude. In our studies, the herbicidal ionic liquids [C ,C ,C ,C N][MCPP] and [C ,C ,C ,C N][dicamba] showed the lowest toxicity among analyzed quaternary ammonium salts and comparable toxicity with corresponding herbicides. No clear increase in toxicity could be followed by changing the anion moieties for ammonium-based ionic liquid forms.
[Mh] Termos MeSH primário: Dicamba/toxicidade
Herbicidas/toxicidade
Líquidos Iônicos/toxicidade
Pseudomonas putida/efeitos dos fármacos
Compostos de Amônio Quaternário/toxicidade
[Mh] Termos MeSH secundário: Pseudomonas putida/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Herbicides); 0 (Ionic Liquids); 0 (Quaternary Ammonium Compounds); SJG3M6RY6H (Dicamba)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170916
[Lr] Data última revisão:
170916
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161008
[St] Status:MEDLINE


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[PMID]:27801844
[Au] Autor:Sabetta W; Crosatti C; Soltész A; Di Rienzo V; Montemurro C
[Ad] Endereço:Sinagri S.r.l., Spin-off, University of Bari, Via Amendola 165/A, Bari 70126, Italy. wilma.sabetta@libero.it.
[Ti] Título:Screening Auxin Response, In Vitro Culture Aptitude and Susceptibility to Agrobacterium-Mediated Transformation of Italian Commercial Durum Wheat Varieties.
[So] Source:Molecules;21(11), 2016 Oct 28.
[Is] ISSN:1420-3049
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:The development of a robust -mediated transformation protocol for a recalcitrant species like durum wheat requires the identification and optimization of factors affecting T-DNA delivery and plant regeneration. The purpose of this research was to compare the behavior of diverse durum wheat genotypes during in vitro culture and -mediated transformation, using immature embryos as explants. Apart from plant genotype, two of the main influencing factors for a successful genetic transformation have been examined here, i.e., auxin source (Dicamba and 2,4-D) and duration of the pre-culture period (one, seven and 21 days). The addition of Dicamba to the media in combination with seven days pre-cultivation resulted in a general enhancement of T-DNA delivery for most of the analyzed cultivars, as revealed by ß-glucuronidase (GUS) histochemical assay. Although all genotypes were able to produce calli, significant differences were detected in regeneration and transformation efficiencies, since only two (Karalis and Neolatino) out of 14 cultivars produced fertile transgenic plants. The estimated transformation efficiencies were 6.25% and 1.66% for Karalis and Neolatino, respectively, and χ² analysis revealed the stable integration and segregation of the transgene in T1 and T2 progenies. This research has demonstrated that, among the influencing factors, genotype and auxin type play the most important role in the success of durum wheat transformation.
[Mh] Termos MeSH primário: Agrobacterium tumefaciens/fisiologia
DNA Bacteriano/genética
Ácidos Indolacéticos/farmacologia
Triticum/genética
[Mh] Termos MeSH secundário: Ácido 2,4-Diclorofenoxiacético/farmacologia
Dicamba/farmacologia
Genótipo
Plantas Geneticamente Modificadas/crescimento & desenvolvimento
Plantas Geneticamente Modificadas/microbiologia
Técnicas de Cultura de Tecidos
Transformação Genética
Triticum/crescimento & desenvolvimento
Triticum/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Indoleacetic Acids); 0 (T-DNA); 2577AQ9262 (2,4-Dichlorophenoxyacetic Acid); SJG3M6RY6H (Dicamba)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170417
[Lr] Data última revisão:
170417
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161102
[St] Status:MEDLINE


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[PMID]:27575686
[Au] Autor:Wang C; Glenn KC; Kessenich C; Bell E; Burzio LA; Koch MS; Li B; Silvanovich A
[Ad] Endereço:Monsanto Company, 800 North Lindbergh Blvd, St. Louis, MO 63167, USA. Electronic address: cunxi.wang@monsanto.com.
[Ti] Título:Safety assessment of dicamba mono-oxygenases that confer dicamba tolerance to various crops.
[So] Source:Regul Toxicol Pharmacol;81:171-182, 2016 Nov.
[Is] ISSN:1096-0295
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Dicamba tolerant (DT) soybean, cotton and maize were developed through constitutive expression of dicamba mono-oxygenase (DMO) in chloroplasts. DMO expressed in three DT crops exhibit 91.6-97.1% amino acid sequence identity to wild type DMO. All DMO forms maintain the characteristics of Rieske oxygenases that have a history of safe use. Additionally, they are all functionally similar in vivo since the three DT crops are all tolerant to dicamba treatment. None of these DMO sequences were found to have similarity to any known allergens or toxins. Herein, to further understand the safety of these DMO variants, a weight of evidence approach was employed. Each purified DMO protein was found to be completely deactivated in vitro by heating at temperatures 55 °C and above, and all were completely digested within 30 s or 5 min by pepsin and pancreatin, respectively. Mice orally dosed with each of these DMO proteins showed no adverse effects as evidenced by analysis of body weight gain, food consumption and clinical observations. Therefore, the weight of evidence from all these protein safety studies support the conclusion that the various forms of DMO proteins introduced into DT soybean, cotton and maize are safe for food and feed consumption, and the small amino acid sequence differences outside the active site of DMO do not raise any additional safety concerns.
[Mh] Termos MeSH primário: Produtos Agrícolas/toxicidade
Dicamba/farmacologia
Resistência a Medicamentos
Alimentos Geneticamente Modificados/toxicidade
Gossypium/toxicidade
Herbicidas/farmacologia
Oxigenases de Função Mista/toxicidade
Oxirredutases O-Desmetilantes/toxicidade
Plantas Geneticamente Modificadas/toxicidade
Feijão de Soja/toxicidade
Zea mays/toxicidade
[Mh] Termos MeSH secundário: Administração Oral
Sequência de Aminoácidos
Animais
Biologia Computacional
Segurança de Produtos ao Consumidor
Produtos Agrícolas/enzimologia
Produtos Agrícolas/genética
Bases de Dados de Proteínas
Resistência a Medicamentos/genética
Estabilidade Enzimática
Feminino
Inocuidade dos Alimentos
Alimentos Geneticamente Modificados/parasitologia
Regulação da Expressão Gênica de Plantas
Gossypium/enzimologia
Gossypium/genética
Seres Humanos
Masculino
Camundongos
Oxigenases de Função Mista/administração & dosagem
Oxigenases de Função Mista/genética
Oxigenases de Função Mista/metabolismo
Pancreatina/metabolismo
Pepsina A/metabolismo
Plantas Geneticamente Modificadas/enzimologia
Plantas Geneticamente Modificadas/genética
Desnaturação Proteica
Proteólise
Proteínas Recombinantes/genética
Proteínas Recombinantes/metabolismo
Proteínas Recombinantes/toxicidade
Medição de Risco
Feijão de Soja/enzimologia
Feijão de Soja/genética
Stenotrophomonas maltophilia/enzimologia
Stenotrophomonas maltophilia/genética
Temperatura Ambiente
Testes de Toxicidade Aguda
Zea mays/enzimologia
Zea mays/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Herbicides); 0 (Recombinant Proteins); 8049-47-6 (Pancreatin); EC 1.- (Mixed Function Oxygenases); EC 1.- (Oxidoreductases, O-Demethylating); EC 1.- (dicamba O-demethylase); EC 3.4.23.1 (Pepsin A); SJG3M6RY6H (Dicamba)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170324
[Lr] Data última revisão:
170324
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160831
[St] Status:MEDLINE


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[PMID]:27537419
[Au] Autor:Kumar V; Jha P
[Ad] Endereço:Montana State University-Bozeman, Department of Research Centers, Southern Agricultural Research Center, Huntley, Montana, 59037, United States of America.
[Ti] Título:Differences in Germination, Growth, and Fecundity Characteristics of Dicamba-Fluroxypyr-Resistant and Susceptible Kochia scoparia.
[So] Source:PLoS One;11(8):e0161533, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The widespread occurrence of herbicide-resistant (HR) Kochia scoparia is an increasing concern for growers in the US Great Plains and Canada. K. scoparia populations resistant to dicamba have been reported in six US states. Populations cross-resistant to dicamba and fluroxypyr have been reported from wheat fields in Montana, USA. It is unclear whether resistance to the auxinic herbicides (dicamba and/or fluroxypyr), can alter the fitness traits of K. scoparia. The objectives of this research were to compare the germination dynamics in response to thermal environment, vegetative growth and fecundity characteristics, and the relative competitive ability of dicamba-fluroxypyr-susceptible (S) vs.-resistant (R) K. scoparia selected from within a single segregating population (collected from wheat-fallow field in MT). S and R selected lines were developed after three generations of recurrent group selection. Compared to the S selected line, the R selected line had lower cumulative germination at all constant temperatures except 25°C, and at all alternating temperatures except 30/35°C. Also, the R selected line had delayed germination relative to the S selected line. The R had lower plant height, plant width, primary branches, total leaf area, stem diameter, and shoot dry weight compared with the S plants in the absence of competition. The reduction in seed production per plant resulted in a 39% fitness cost. The 1000-seed weight of R (1.6 g) was also less than that of S (2.6 g). When grown in an intraspecific competition at different mixture proportions, replacement series indices for the growth parameters further indicated that the R was less competitive than the S. Evident from this research, the dicamba-fluroxypyr-resistant R selected line is less likely to persist in a field population in the absence of the auxinic herbicides.
[Mh] Termos MeSH primário: Acetatos/farmacologia
Bassia scoparia/fisiologia
Dicamba/farmacologia
Germinação/fisiologia
Resistência a Herbicidas
Herbicidas/farmacologia
Piridinas/farmacologia
[Mh] Termos MeSH secundário: Bassia scoparia/efeitos dos fármacos
Bassia scoparia/crescimento & desenvolvimento
Fertilidade/fisiologia
Resistência a Herbicidas/fisiologia
Sementes/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acetates); 0 (Herbicides); 0 (Pyridines); 8O40SHO197 (4,5-amino-3,5-dichloro-6-fluoro-2-pyridinyloxyacetic acid); SJG3M6RY6H (Dicamba)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160819
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0161533


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[PMID]:27422839
[Au] Autor:Yao L; Yu LL; Zhang JJ; Xie XT; Tao Q; Yan X; Hong Q; Qiu JG; He J; Ding DR
[Ad] Endereço:Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, Jiangsu, People's Republic of China.
[Ti] Título:A Tetrahydrofolate-Dependent Methyltransferase Catalyzing the Demethylation of Dicamba in Sphingomonas sp. Strain Ndbn-20.
[So] Source:Appl Environ Microbiol;82(18):5621-30, 2016 Sep 15.
[Is] ISSN:1098-5336
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:UNLABELLED: Sphingomonas sp. strain Ndbn-20 degrades and utilizes the herbicide dicamba as its sole carbon and energy source. In the present study, a tetrahydrofolate (THF)-dependent dicamba methyltransferase gene, dmt, was cloned from the strain, and three other genes, metF, dhc, and purU, which are involved in THF metabolism, were found to be located downstream of dmt A transcriptional study revealed that the four genes constituted one transcriptional unit that was constitutively transcribed. Lysates of cells grown with glucose or dicamba exhibited almost the same activities, which further suggested that the dmt gene is constitutively expressed in the strain. Dmt shared 46% and 45% identities with the methyltransferases DesA and LigM from Sphingomonas paucimobilis SYK-6, respectively. The purified Dmt catalyzed the transfer of methyl from dicamba to THF to form the herbicidally inactive metabolite 3,6-dichlorosalicylic acid (DCSA) and 5-methyl-THF. The activity of Dmt was inhibited by 5-methyl-THF but not by DCSA. The introduction of a codon-optimized dmt gene into Arabidopsis thaliana enhanced resistance against dicamba. In conclusion, this study identified a THF-dependent dicamba methyltransferase, Dmt, with potential applications for the genetic engineering of dicamba-resistant crops. IMPORTANCE: Dicamba is a very important herbicide that is widely used to control more than 200 types of broadleaf weeds and is a suitable target herbicide for the engineering of herbicide-resistant transgenic crops. A study of the mechanism of dicamba metabolism by soil microorganisms will benefit studies of its dissipation, transformation, and migration in the environment. This study identified a THF-dependent methyltransferase, Dmt, capable of catalyzing dicamba demethylation in Sphingomonas sp. Ndbn-20, and a preliminary study of its enzymatic characteristics was performed. Introduction of a codon-optimized dmt gene into Arabidopsis thaliana enhanced resistance against dicamba, suggesting that the dmt gene has potential applications for the genetic engineering of herbicide-resistant crops.
[Mh] Termos MeSH primário: Dicamba/metabolismo
Metiltransferases/metabolismo
Sphingomonas/enzimologia
Sphingomonas/metabolismo
Tetra-Hidrofolatos/metabolismo
[Mh] Termos MeSH secundário: Arabidopsis/efeitos dos fármacos
Arabidopsis/genética
Biotransformação
Carbono/metabolismo
Clonagem Molecular
Metabolismo Energético
Perfilação da Expressão Gênica
Resistência a Herbicidas
Metiltransferases/genética
Metiltransferases/isolamento & purificação
Família Multigênica
Óperon
Plantas Geneticamente Modificadas/efeitos dos fármacos
Plantas Geneticamente Modificadas/genética
Transcrição Genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Tetrahydrofolates); 43ZWB253H4 (5,6,7,8-tetrahydrofolic acid); 7440-44-0 (Carbon); EC 2.1.1.- (Methyltransferases); SJG3M6RY6H (Dicamba)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171009
[Lr] Data última revisão:
171009
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160717
[St] Status:MEDLINE
[do] DOI:10.1128/AEM.01201-16


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[PMID]:27282375
[Au] Autor:Freydier L; Lundgren JG
[Ad] Endereço:Agrocampus Ouest, 65 rue de Saint-Brieuc, CS 842015, 35042, Rennes Cedex, France.
[Ti] Título:Unintended effects of the herbicides 2,4-D and dicamba on lady beetles.
[So] Source:Ecotoxicology;25(6):1270-7, 2016 Aug.
[Is] ISSN:1573-3017
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Weed resistance to glyphosate and development of new GM crops tolerant to 2,4-dichlorophenoxyacetic acid (2,4-D) and dicamba is expected to lead to increased use of these herbicides in cropland. The lady beetle, Coleomegilla maculata is an important beneficial insect in cropland that is commonly used as an indicator species in safety evaluations of pesticides. Here, we examined the lethal and non-lethal effects of 2,4-D and dicamba active ingredients and commercial formulations to this lady beetle species, and tested for synergistic effects of the herbicides. Second instars of lady beetles were exposed to an experimental treatment, and their mortality, development, weight, sex ratio, fecundity, and mobility was evaluated. Using similar methods, a dose-response study was conducted on 2,4-D with and without dicamba. The commercial formulation of 2,4-D was highly lethal to lady beetle larvae; the LC90 of this herbicide was 13 % of the label rate. In this case, the "inactive" ingredients were a key driver of the toxicity. Dicamba active ingredient significantly increased lady beetle mortality and reduced their body weight. The commercial formulations of both herbicides reduced the proportion of males in the lady beetle population. The herbicides when used together did not act synergistically in their toxicity toward lady beetles versus when the chemistries were used independently. Our work shows that herbicide formulations can cause both lethal and sublethal effects on non-target, beneficial insects, and these effects are sometimes driven by the "inactive" ingredients. The field-level implications of shifts in weed management practices on insect management programs should receive further attention.
[Mh] Termos MeSH primário: Ácido 2,4-Diclorofenoxiacético/toxicidade
Coleópteros/efeitos dos fármacos
Dicamba/toxicidade
Herbicidas/toxicidade
[Mh] Termos MeSH secundário: Animais
Produtos Agrícolas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Herbicides); 2577AQ9262 (2,4-Dichlorophenoxyacetic Acid); SJG3M6RY6H (Dicamba)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160611
[St] Status:MEDLINE
[do] DOI:10.1007/s10646-016-1680-4


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[PMID]:27255344
[Au] Autor:Yao L; Zhang JJ; Yu LL; Chen Q; Zhu JC; He J; Ding DR
[Ad] Endereço:1​Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
[Ti] Título:Rhizorhabdus dicambivorans sp. nov., a dicamba-degrading bacterium isolated from compost.
[So] Source:Int J Syst Evol Microbiol;66(9):3317-3323, 2016 Sep.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Strain Ndbn-20T, a Gram-staining-negative, non-spore-forming bacterium, was isolated from compost of plant litter. The strain was able to degrade dicamba. Phylogenetic analysis based on 16S rRNA gene sequences indicated that Ndbn-20Trepresented a member of the family Sphingomonadaceae of the Alphaproteobacteria and showed high sequence similarities to Rhizorhabdusargentea SP1T (98.8 %), Sphingomonaswittichii RW1T (97.9 %), Sphingomonasstarnbergensis 382T (97.7 %) and Sphingomonashistidinilytica UM2T (97.7 %). However, the strain showed low DNA sequence relatedness with R. argentea SP1T (45.6±1.9 %), S. wittichii RW1T (33.5±2.3 %), S.histidinilytica UM2T (39.4±3.6 %) and S. starnbergensis382T (42.1±4.1 %). Ndbn-20T possessed Q-10 as the predominant ubiquinone, spermidine as the major polyamine, and summed feature 8 (comprising C18 : 1ω7c/C18 : 1ω6c), summed feature 3 (comprising C16 : 1ω7c/C16 : 1ω6c), C17 : 1ω6c, C16 : 0 and C14 : 02-OH as the major fatty acids (>5 % of the total). The profile of polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, glycolipid, sphingoglycolipid, phosphatidyldimethylethanolamine and phosphatidylglycerol. The DNA G+C content was 65.4 mol%. Based on a polyphasic taxonomic analysis, strain Ndbn-20T is proposed to represent a novel species of the genus Rhizorhabdus, with the proposed name of Rhizorhabdus dicambivorans sp. nov. The type strain is Ndbn-20T (=CCTCC AB 2016143=KACC 18661).
[Mh] Termos MeSH primário: Dicamba/metabolismo
Filogenia
Microbiologia do Solo
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
China
DNA Bacteriano/genética
Ácidos Graxos/química
Hibridização de Ácido Nucleico
Fosfolipídeos/química
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Espermidina/química
Sphingomonadaceae/classificação
Ubiquinona/análogos & derivados
Ubiquinona/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S); 1339-63-5 (Ubiquinone); EJ27X76M46 (coenzyme Q10); SJG3M6RY6H (Dicamba); U87FK77H25 (Spermidine)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160604
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.001194



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