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Pesquisa : D02.241.223.100.300.595.608 [Categoria DeCS]
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  1 / 5148 MEDLINE  
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[PMID]:28881317
[Au] Autor:Kaur Kohli S; Handa N; Bali S; Arora S; Sharma A; Kaur R; Bhardwaj R
[Ad] Endereço:Department of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar 143005, India.
[Ti] Título:Modulation of antioxidative defense expression and osmolyte content by co-application of 24-epibrassinolide and salicylic acid in Pb exposed Indian mustard plants.
[So] Source:Ecotoxicol Environ Saf;147:382-393, 2018 Jan.
[Is] ISSN:1090-2414
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The study focuses on potential of combined pre-soaking treatment of 24-Epibrassinolide (EBL) and Salicylic acid (SA) in alleviating Pb phytotoxicity in Brassica juncea L. plants. The seeds after treatment with combination of both the hormones were sown in mixture of soil, sand and manure (3:1:1) and were exposed to Pb concentrations (0.25mM, 0.50mM and 0.75mM). After 30 days of growth, the plants were harvested and processed, for quantification of various metabolites. It was found that pre-sowing of seeds in combination of EBL and SA, mitigated the adverse effects of metal stress by modulating antioxidative defense response and enhanced osmolyte contents. Dry matter content and heavy metal tolerance index were enhanced in response to co-application of EBL and SA. The levels of superoxide anions, hydrogen peroxide and malondialdehyde were lowered by the combined treatment of hormones. Enhancement in activities of guaiacol peroxidase, catalase, glutathione reductase and glutathione-s-transferase were recorded. Contents of glutathione, tocopherol and ascorbic acid were also enhanced in response to co-application of both hormones. Expression of POD, CAT, GR and GST1 genes were up-regulated whereas SOD gene was observed to be down-regulated. Contents of proline, trehalose and glycine betaine were also reported to be elevated as a result of treatment with EBL+SA. The results suggest that co-application of EBL+SA may play an imperative role in improving the antioxidative defense expression of B. juncea plants to combat the oxidative stress generated by Pb toxicity.
[Mh] Termos MeSH primário: Antioxidantes/metabolismo
Brassinosteroides/farmacologia
Poluentes Ambientais/toxicidade
Chumbo/toxicidade
Mostardeira/efeitos dos fármacos
Ácido Salicílico/farmacologia
Esteroides Heterocíclicos/farmacologia
[Mh] Termos MeSH secundário: Poluentes Ambientais/metabolismo
Expressão Gênica/efeitos dos fármacos
Hidroponia
Chumbo/metabolismo
Mostardeira/enzimologia
Mostardeira/genética
Osmorregulação/efeitos dos fármacos
Estresse Oxidativo/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Brassinosteroids); 0 (Environmental Pollutants); 0 (Steroids, Heterocyclic); 2P299V784P (Lead); O414PZ4LPZ (Salicylic Acid); Y9IQ1L53OX (brassinolide)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170908
[St] Status:MEDLINE


  2 / 5148 MEDLINE  
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[PMID]:29253890
[Au] Autor:Patharkar OR; Gassmann W; Walker JC
[Ad] Endereço:Division of Biological Sciences and Interdisciplinary Plant Group, University of Missouri, Columbia, MO, United States of America.
[Ti] Título:Leaf shedding as an anti-bacterial defense in Arabidopsis cauline leaves.
[So] Source:PLoS Genet;13(12):e1007132, 2017 12.
[Is] ISSN:1553-7404
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Plants utilize an innate immune system to protect themselves from disease. While many molecular components of plant innate immunity resemble the innate immunity of animals, plants also have evolved a number of truly unique defense mechanisms, particularly at the physiological level. Plant's flexible developmental program allows them the unique ability to simply produce new organs as needed, affording them the ability to replace damaged organs. Here we develop a system to study pathogen-triggered leaf abscission in Arabidopsis. Cauline leaves infected with the bacterial pathogen Pseudomonas syringae abscise as part of the defense mechanism. Pseudomonas syringae lacking a functional type III secretion system fail to elicit an abscission response, suggesting that the abscission response is a novel form of immunity triggered by effectors. HAESA/HAESA-like 2, INFLORESCENCE DEFICIENT IN ABSCISSION, and NEVERSHED are all required for pathogen-triggered abscission to occur. Additionally phytoalexin deficient 4, enhanced disease susceptibility 1, salicylic acid induction-deficient 2, and senescence-associated gene 101 plants with mutations in genes necessary for bacterial defense and salicylic acid signaling, and NahG transgenic plants with low levels of salicylic acid fail to abscise cauline leaves normally. Bacteria that physically contact abscission zones trigger a strong abscission response; however, long-distance signals are also sent from distal infected tissue to the abscission zone, alerting the abscission zone of looming danger. We propose a threshold model regulating cauline leaf defense where minor infections are handled by limiting bacterial growth, but when an infection is deemed out of control, cauline leaves are shed. Together with previous results, our findings suggest that salicylic acid may regulate both pathogen- and drought-triggered leaf abscission.
[Mh] Termos MeSH primário: Arabidopsis/fisiologia
Folhas de Planta/genética
Folhas de Planta/fisiologia
[Mh] Termos MeSH secundário: Arabidopsis/genética
Proteínas de Arabidopsis/genética
Flores/genética
Genes de Plantas
Imunidade Inata
Inflorescência/genética
Mutação
Folhas de Planta/microbiologia
Plantas Geneticamente Modificadas
Proteínas Serina-Treonina Quinases/genética
Pseudomonas syringae/genética
Pseudomonas syringae/isolamento & purificação
Ácido Salicílico
Transdução de Sinais/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Arabidopsis Proteins); EC 2.7.11.1 (Protein-Serine-Threonine Kinases); O414PZ4LPZ (Salicylic Acid)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180227
[Lr] Data última revisão:
180227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171219
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pgen.1007132


  3 / 5148 MEDLINE  
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[PMID]:28470514
[Au] Autor:Ates G; Vanhaecke T; Rogiers V; Rodrigues RM
[Ad] Endereço:Department of In Vitro Toxicology and Dermato-Cosmetology, Faculty of Medicine and Pharmacy, Vrije Universiteit Brussel, Brussels, Belgium.
[Ti] Título:Assaying Cellular Viability Using the Neutral Red Uptake Assay.
[So] Source:Methods Mol Biol;1601:19-26, 2017.
[Is] ISSN:1940-6029
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The neutral red uptake assay is a cell viability assay that allows in vitro quantification of xenobiotic-induced cytotoxicity. The assay relies on the ability of living cells to incorporate and bind neutral red, a weak cationic dye, in lysosomes. As such, cytotoxicity is expressed as a concentration-dependent reduction of the uptake of neutral red after exposure to the xenobiotic under investigation. The neutral red uptake assay is mainly used for hazard assessment in in vitro toxicology applications. This method has also been introduced in regulatory recommendations as part of 3T3-NRU-phototoxicity-assay, which was regulatory accepted in all EU member states in 2000 and in the OECD member states in 2004 as a test guideline (TG 432). The present protocol describes the neutral red uptake assay using the human hepatoma cell line HepG2, which is often employed as an alternative in vitro model for human hepatocytes. As an example, the cytotoxicity of acetaminophen and acetyl salicylic acid is assessed.
[Mh] Termos MeSH primário: Sobrevivência Celular/efeitos dos fármacos
Corantes/metabolismo
Hepatócitos/efeitos dos fármacos
Vermelho Neutro/metabolismo
Testes de Toxicidade/métodos
Xenobióticos/toxicidade
[Mh] Termos MeSH secundário: Células 3T3
Acetaminofen/toxicidade
Animais
Bioensaio
Células Hep G2
Hepatócitos/metabolismo
Seres Humanos
Modelos Logísticos
Lisossomos/efeitos dos fármacos
Lisossomos/metabolismo
Camundongos
Organização para a Cooperação e Desenvolvimento Econômico
Ácido Salicílico/toxicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Coloring Agents); 0 (Xenobiotics); 261QK3SSBH (Neutral Red); 362O9ITL9D (Acetaminophen); O414PZ4LPZ (Salicylic Acid)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180219
[Lr] Data última revisão:
180219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1007/978-1-4939-6960-9_2


  4 / 5148 MEDLINE  
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[PMID]:29231735
[Au] Autor:Puthusseri B; Divya P; Lokesh V; Kumar G; Savanur MA; Neelwarne B
[Ad] Endereço:Plant Cell Biotechnology Department, CSIR-Central Food Technological Research Institute , Mysore 570020, India.
[Ti] Título:Novel Folate Binding Protein in Arabidopsis Expressed during Salicylic Acid-Induced Folate Accumulation.
[So] Source:J Agric Food Chem;66(2):505-511, 2018 Jan 17.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Increasing the quantity of natural folates in plant foods is recently gaining significant interest, owing to their acute deficiencies in various populations. This study observed that foliar salicylic acid treatment enhanced the accumulation of folates in Arabidopsis, which correlated with the increase in a folate binding protein (FBP) and the expression of mRNA of a putative folate binding protein At5G27830. A protein band corresponding to ∼43 kDa was observed after resolving the affinity-purified protein on SDS-PAGE, and the partial amino acid sequence indicated that the protein is indeed At5G27830. Docking studies performed with At5G27830 confirmed specific binding of folic acid to predicted site. Heterologous expression of At5G27830 in the yeast resulted in significant uptake and accumulation of folic acid in cells. This novel study of a plant FBP will be useful for folate metabolic engineering of a wide range of crops.
[Mh] Termos MeSH primário: Proteínas de Arabidopsis/metabolismo
Arabidopsis/metabolismo
Proteínas de Transporte/metabolismo
Ácido Fólico/metabolismo
Reguladores de Crescimento de Planta/farmacologia
Ácido Salicílico/farmacologia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Arabidopsis/química
Arabidopsis/efeitos dos fármacos
Arabidopsis/genética
Proteínas de Arabidopsis/química
Proteínas de Arabidopsis/genética
Proteínas de Transporte/química
Proteínas de Transporte/genética
Peso Molecular
Alinhamento de Sequência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arabidopsis Proteins); 0 (Carrier Proteins); 0 (Plant Growth Regulators); 935E97BOY8 (Folic Acid); O414PZ4LPZ (Salicylic Acid)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180205
[Lr] Data última revisão:
180205
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171213
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b04236


  5 / 5148 MEDLINE  
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[PMID]:29235833
[Au] Autor:Molodchenkova OO; Adamovskaya VG; Ciselskaya LY; Bezkrovnaya LY; Kartuzova TV; Iablonska VB
[Ti] Título:Purification and properties of lipoxygenase from wheat seedlings infected by Fusarium graminearum and treated by salicylic acid.
[So] Source:Ukr Biochem J;88(6):26-34, 2016 Nov-Dec.
[Is] ISSN:2409-4943
[Cp] País de publicação:Ukraine
[La] Idioma:eng
[Ab] Resumo:Lipoxygenase from wheat seedlings in normal conditions, infected by Fusarium graminearum and treated by salicylic acid was isolated. The isolated enzyme was purified by the methods of salting-out (60% ammonium sulphate), dialysis, gel-filtration and ion-exchange chromatography. Specific activity of the purified enzyme was 8.0-12.5 ΔЕ234/mg of protein, degree of purification ­ 11.6-15.3 times. The enzyme yield was 18.3-27.9%. Molecular mass of lipoxygenase is 90 kDa, amino acid composition is distinguished by a high content of glutamic acid, proline, valine, isoleucine, leucine and low level of histidine, tyrosine, phenylalanine, threonine, tryptophan, cystein. Research of lipoxygenase substrate dependence indicated that the enzyme catalysed with the maximum velocity of the reaction of arachidonic acid oxidation at a substrate concentration of 4.5 mM at pH 7.2, the reaction of linoleic acid oxidation at a substrate concentration of 4.5 mM at pH 7.2 and the reaction of linolenic acid oxidation at a substrate concentration of 9.0 mM at pH 8.0. The change of wheat lipoxygenase activity depending on genotype resistance to Fusarium graminearum and millieu of germination was shown. One of the manifestations of the protective effect of salicylic acid is its ability to induce changes of lipoxygenase activity.
[Mh] Termos MeSH primário: Fungicidas Industriais/farmacologia
Lipoxigenase/isolamento & purificação
Proteínas de Plantas/isolamento & purificação
Ácido Salicílico/farmacologia
Triticum/efeitos dos fármacos
[Mh] Termos MeSH secundário: Aminoácidos/química
Ácido Araquidônico/metabolismo
Cromatografia por Troca Iônica
Resistência à Doença
Suscetibilidade a Doenças/enzimologia
Suscetibilidade a Doenças/imunologia
Fusarium/efeitos dos fármacos
Fusarium/crescimento & desenvolvimento
Fusarium/patogenicidade
Expressão Gênica
Concentração de Íons de Hidrogênio
Cinética
Ácido Linoleico/metabolismo
Lipoxigenase/metabolismo
Peso Molecular
Doenças das Plantas/imunologia
Doenças das Plantas/microbiologia
Doenças das Plantas/prevenção & controle
Proteínas de Plantas/metabolismo
Plântulas/efeitos dos fármacos
Plântulas/enzimologia
Plântulas/imunologia
Plântulas/microbiologia
Especificidade por Substrato
Triticum/enzimologia
Triticum/imunologia
Triticum/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amino Acids); 0 (Fungicides, Industrial); 0 (Plant Proteins); 27YG812J1I (Arachidonic Acid); 9KJL21T0QJ (Linoleic Acid); EC 1.13.11.12 (Lipoxygenase); O414PZ4LPZ (Salicylic Acid)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180116
[Lr] Data última revisão:
180116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171214
[St] Status:MEDLINE
[do] DOI:10.15407/ubj88.06.026


  6 / 5148 MEDLINE  
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[PMID]:29182277
[Au] Autor:Keszycka PK; Szkop M; Gajewska D
[Ad] Endereço:Department of Dietetics, Faculty of Human Nutrition and Consumer Sciences, and ‡Department of Biochemistry, Faculty of Agriculture and Biology, Warsaw University of Life Sciences-SGGW , Nowoursynowska 159, 02-776 Warsaw, Poland.
[Ti] Título:Overall Content of Salicylic Acid and Salicylates in Food Available on the European Market.
[So] Source:J Agric Food Chem;65(50):11085-11091, 2017 Dec 20.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The study aimed to determine the salicylates content in 112 products available on the European market. Quantitative determination of free and conjugated forms of salicylic acid in food was performed using reversed-phase high-performance liquid chromatography with fluorescence detection. The salicylates contents ranged from 0 to 1675.79 (µg/100 g). The results of this study confirm the presence of salicylates in food products, as well as a broad content diversity of these compounds depending on the species, variety, and method of processing the food items. The results can be very helpful for nutritionists and dieticians in planning low-salicylates or high-salicylates diets.
[Mh] Termos MeSH primário: Carne/análise
Óvulo/química
Extratos Vegetais/análise
Plantas/química
Salicilatos/análise
Ácido Salicílico/análise
[Mh] Termos MeSH secundário: Animais
Cromatografia Líquida de Alta Pressão
Europa (Continente)
Alimentos/economia
Análise de Alimentos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Extracts); 0 (Salicylates); O414PZ4LPZ (Salicylic Acid)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180110
[Lr] Data última revisão:
180110
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171129
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b04313


  7 / 5148 MEDLINE  
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[PMID]:29073135
[Au] Autor:Matsui H; Nomura Y; Egusa M; Hamada T; Hyon GS; Kaminaka H; Watanabe Y; Ueda T; Trujillo M; Shirasu K; Nakagami H
[Ad] Endereço:RIKEN Center for Sustainable Resource Science, Yokohama, Japan.
[Ti] Título:The GYF domain protein PSIG1 dampens the induction of cell death during plant-pathogen interactions.
[So] Source:PLoS Genet;13(10):e1007037, 2017 Oct.
[Is] ISSN:1553-7404
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The induction of rapid cell death is an effective strategy for plants to restrict biotrophic and hemi-biotrophic pathogens at the infection site. However, activation of cell death comes at a high cost, as dead cells will no longer be available for defense responses nor general metabolic processes. In addition, necrotrophic pathogens that thrive on dead tissue, take advantage of cell death-triggering mechanisms. Mechanisms by which plants solve this conundrum remain described. Here, we identify PLANT SMY2-TYPE ILE-GYF DOMAIN-CONTAINING PROTEIN 1 (PSIG1) and show that PSIG1 helps to restrict cell death induction during pathogen infection. Inactivation of PSIG1 does not result in spontaneous lesions, and enhanced cell death in psig1 mutants is independent of salicylic acid (SA) biosynthesis or reactive oxygen species (ROS) production. Moreover, PSIG1 interacts with SMG7, which plays a role in nonsense-mediated RNA decay (NMD), and the smg7-4 mutant allele mimics the cell death phenotype of the psig1 mutants. Intriguingly, the psig1 mutants display enhanced susceptibility to the hemi-biotrophic bacterial pathogen. These findings point to the existence and importance of the SA- and ROS-independent cell death constraining mechanism as a part of the plant immune system.
[Mh] Termos MeSH primário: Proteínas de Arabidopsis/genética
Arabidopsis/genética
Proteínas de Transporte/genética
Interações Hospedeiro-Patógeno/genética
[Mh] Termos MeSH secundário: Arabidopsis/crescimento & desenvolvimento
Morte Celular/genética
Regulação da Expressão Gênica de Plantas
Degradação do RNAm Mediada por Códon sem Sentido
Doenças das Plantas/genética
Doenças das Plantas/microbiologia
Domínios Proteicos/genética
Espécies Reativas de Oxigênio/metabolismo
Ácido Salicílico/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arabidopsis Proteins); 0 (Carrier Proteins); 0 (PSIG1 protein, Arabidopsis); 0 (Reactive Oxygen Species); 0 (SMG7 protein, Arabidopsis); O414PZ4LPZ (Salicylic Acid)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171110
[Lr] Data última revisão:
171110
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171027
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pgen.1007037


  8 / 5148 MEDLINE  
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[PMID]:28973025
[Au] Autor:Poncini L; Wyrsch I; Dénervaud Tendon V; Vorley T; Boller T; Geldner N; Métraux JP; Lehmann S
[Ad] Endereço:Department of Biology, University of Fribourg, Fribourg, Switzerland.
[Ti] Título:In roots of Arabidopsis thaliana, the damage-associated molecular pattern AtPep1 is a stronger elicitor of immune signalling than flg22 or the chitin heptamer.
[So] Source:PLoS One;12(10):e0185808, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Plants interpret their immediate environment through perception of small molecules. Microbe-associated molecular patterns (MAMPs) such as flagellin and chitin are likely to be more abundant in the rhizosphere than plant-derived damage-associated molecular patterns (DAMPs). We investigated how the Arabidopsis thaliana root interprets MAMPs and DAMPs as danger signals. We monitored root development during exposure to increasing concentrations of the MAMPs flg22 and the chitin heptamer as well as of the DAMP AtPep1. The tissue-specific expression of defence-related genes in roots was analysed using a toolkit of promoter::YFPN lines reporting jasmonic acid (JA)-, salicylic acid (SA)-, ethylene (ET)- and reactive oxygen species (ROS)- dependent signalling. Finally, marker responses were analysed during invasion by the root pathogen Fusarium oxysporum. The DAMP AtPep1 triggered a stronger activation of the defence markers compared to flg22 and the chitin heptamer. In contrast to the tested MAMPs, AtPep1 induced SA- and JA-signalling markers in the root and caused a severe inhibition of root growth. Fungal attack resulted in a strong activation of defence genes in tissues close to the invading fungal hyphae. The results collectively suggest that AtPep1 presents a stronger danger signal to the Arabidopsis root than the MAMPs flg22 and chitin heptamer.
[Mh] Termos MeSH primário: Proteínas de Arabidopsis/metabolismo
Quitina/metabolismo
Flagelina/metabolismo
Raízes de Plantas/metabolismo
Transdução de Sinais/fisiologia
Transativadores/metabolismo
[Mh] Termos MeSH secundário: Proteínas de Arabidopsis/genética
Quitina/genética
Ciclopentanos/metabolismo
Etilenos/metabolismo
Flagelina/genética
Regulação da Expressão Gênica de Plantas
Oxilipinas/metabolismo
Raízes de Plantas/crescimento & desenvolvimento
Espécies Reativas de Oxigênio/metabolismo
Ácido Salicílico/metabolismo
Transativadores/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arabidopsis Proteins); 0 (Cyclopentanes); 0 (Ethylenes); 0 (Oxylipins); 0 (Pep1 protein, Arabidopsis); 0 (Reactive Oxygen Species); 0 (Trans-Activators); 12777-81-0 (Flagellin); 1398-61-4 (Chitin); 6RI5N05OWW (jasmonic acid); 91GW059KN7 (ethylene); O414PZ4LPZ (Salicylic Acid)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171019
[Lr] Data última revisão:
171019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171004
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0185808


  9 / 5148 MEDLINE  
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[PMID]:28818753
[Au] Autor:Kamatham S; Pallu R; Pasupulati AK; Singh SS; Gudipalli P
[Ad] Endereço:Department of Biochemistry, School of Life Sciences, University of Hyderabad, Hyderabad, 500046, Telangana, India.
[Ti] Título:Benzoylsalicylic acid derivatives as defense activators in tobacco and Arabidopsis.
[So] Source:Phytochemistry;143:160-169, 2017 Nov.
[Is] ISSN:1873-3700
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Systemic acquired resistance (SAR) is a long lasting inducible whole plant immunity often induced by either pathogens or chemical elicitors. Salicylic acid (SA) is a known SAR signal against a broad spectrum of pathogens in plants. In a recent study, we have reported that benzoylsalicylic acid (BzSA) is a SAR inducer in tobacco and Arabidopsis plants. Here, we have synthesized BzSA derivatives using SA and benzoyl chlorides of various moieties as substrates. The chemical structures of BzSA derivatives were elucidated using Infrared spectroscopy (IR), Nuclear magnetic spectroscopy (NMR) and High-resolution mass spectrometer (HRMS) analysis. The bioefficacy of BzSA derivatives in inducing defense response against tobacco mosaic virus (TMV) was investigated in tobacco and SA abolished transgenic NahG Arabidopsis plants. Interestingly, pre-treatment of local leaves of tobacco with BzSA derivatives enhanced the expression of SAR genes such as NPR1 [Non-expressor of pathogenesis-related (PR) genes 1], PR and other defense marker genes (HSR203, SIPK, WIPK) in systemic leaves. Pre-treatment of BzSA derivatives reduced the spread of TMV infection to uninfected areas by restricting lesion number and diameter both in local and systemic leaves of tobacco in a dose-dependent manner. Furthermore, pre-treatment of BzSA derivatives in local leaves of SA deficient Arabidopsis NahG plants induced SAR through AtPR1 and AtPR5 gene expression and reduced leaf necrosis and curling symptoms in systemic leaves as compared to BzSA. These results suggest that BzSA derivatives are potent SAR inducers against TMV in tobacco and Arabidopsis.
[Mh] Termos MeSH primário: Arabidopsis/metabolismo
Salicilatos/farmacologia
Tabaco/metabolismo
[Mh] Termos MeSH secundário: Arabidopsis/genética
Índia
Estrutura Molecular
Ressonância Magnética Nuclear Biomolecular
Plantas Geneticamente Modificadas
Salicilatos/química
Ácido Salicílico/metabolismo
Tabaco/genética
Vírus do Mosaico do Tabaco
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Salicylates); O414PZ4LPZ (Salicylic Acid)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171002
[Lr] Data última revisão:
171002
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170819
[St] Status:MEDLINE


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[PMID]:28771548
[Au] Autor:Nanjareddy K; Arthikala MK; Gómez BM; Blanco L; Lara M
[Ad] Endereço:Ciencias Agrogenómicas, Escuela Nacional de Estudios Superiores Unidad León- Universidad Nacional Autónoma de México (UNAM), León, Guanajuato, México.
[Ti] Título:Differentially expressed genes in mycorrhized and nodulated roots of common bean are associated with defense, cell wall architecture, N metabolism, and P metabolism.
[So] Source:PLoS One;12(8):e0182328, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Legumes participate in two important endosymbiotic associations, with phosphorus-acquiring arbuscular mycorrhiza (AM, soil fungi) and with nitrogen-fixing bacterial rhizobia. These divergent symbionts share a common symbiotic signal transduction pathway that facilitates the establishment of mycorrhization and nodulation in legumes. However, the unique and shared downstream genes essential for AM and nodule development have not been identified in crop legumes. Here, we used ion torrent next-generation sequencing to perform comparative transcriptomics of common bean (Phaseolus vulgaris) roots colonized by AM or rhizobia. We analyzed global gene expression profiles to identify unique and shared differentially expressed genes (DEGs) that regulate these two symbiotic interactions, and quantitatively compared DEG profiles. We identified 3,219 (1,959 upregulated and 1,260 downregulated) and 2,645 (1,247 upregulated and 1,398 downregulated) unigenes that were differentially expressed in response to mycorrhizal or rhizobial colonization, respectively, compared with uninoculated roots. We obtained quantitative expression profiles of unique and shared genes involved in processes related to defense, cell wall structure, N metabolism, and P metabolism in mycorrhized and nodulated roots. KEGG pathway analysis indicated that most genes involved in jasmonic acid and salicylic acid signaling, N metabolism, and inositol phosphate metabolism are variably expressed during symbiotic interactions. These combined data provide valuable information on symbiotic gene signaling networks that respond to mycorrhizal and rhizobial colonization, and serve as a guide for future genetic strategies to enhance P uptake and N-fixing capacity to increase the net yield of this valuable grain legume.
[Mh] Termos MeSH primário: Regulação da Expressão Gênica de Plantas/genética
Micorrizas/genética
Micorrizas/metabolismo
Nitrogênio/metabolismo
Phaseolus/genética
Phaseolus/metabolismo
Fósforo/metabolismo
[Mh] Termos MeSH secundário: Parede Celular/química
Parede Celular/metabolismo
Ciclopentanos/metabolismo
Fungos/fisiologia
Perfilação da Expressão Gênica
Oxilipinas/metabolismo
Phaseolus/crescimento & desenvolvimento
Phaseolus/microbiologia
Raízes de Plantas/crescimento & desenvolvimento
Raízes de Plantas/metabolismo
Raízes de Plantas/microbiologia
RNA Mensageiro/metabolismo
Rhizobium/fisiologia
Ácido Salicílico/metabolismo
Transdução de Sinais
Simbiose
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cyclopentanes); 0 (Oxylipins); 0 (RNA, Messenger); 27YLU75U4W (Phosphorus); 6RI5N05OWW (jasmonic acid); N762921K75 (Nitrogen); O414PZ4LPZ (Salicylic Acid)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170804
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0182328



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