[PMID]: | 28771548 |
[Au] Autor: | Nanjareddy K; Arthikala MK; Gómez BM; Blanco L; Lara M |
[Ad] Endereço: | Ciencias Agrogenómicas, Escuela Nacional de Estudios Superiores Unidad León- Universidad Nacional Autónoma de México (UNAM), León, Guanajuato, México. |
[Ti] Título: | Differentially expressed genes in mycorrhized and nodulated roots of common bean are associated with defense, cell wall architecture, N metabolism, and P metabolism. |
[So] Source: | PLoS One;12(8):e0182328, 2017. |
[Is] ISSN: | 1932-6203 |
[Cp] País de publicação: | United States |
[La] Idioma: | eng |
[Ab] Resumo: | Legumes participate in two important endosymbiotic associations, with phosphorus-acquiring arbuscular mycorrhiza (AM, soil fungi) and with nitrogen-fixing bacterial rhizobia. These divergent symbionts share a common symbiotic signal transduction pathway that facilitates the establishment of mycorrhization and nodulation in legumes. However, the unique and shared downstream genes essential for AM and nodule development have not been identified in crop legumes. Here, we used ion torrent next-generation sequencing to perform comparative transcriptomics of common bean (Phaseolus vulgaris) roots colonized by AM or rhizobia. We analyzed global gene expression profiles to identify unique and shared differentially expressed genes (DEGs) that regulate these two symbiotic interactions, and quantitatively compared DEG profiles. We identified 3,219 (1,959 upregulated and 1,260 downregulated) and 2,645 (1,247 upregulated and 1,398 downregulated) unigenes that were differentially expressed in response to mycorrhizal or rhizobial colonization, respectively, compared with uninoculated roots. We obtained quantitative expression profiles of unique and shared genes involved in processes related to defense, cell wall structure, N metabolism, and P metabolism in mycorrhized and nodulated roots. KEGG pathway analysis indicated that most genes involved in jasmonic acid and salicylic acid signaling, N metabolism, and inositol phosphate metabolism are variably expressed during symbiotic interactions. These combined data provide valuable information on symbiotic gene signaling networks that respond to mycorrhizal and rhizobial colonization, and serve as a guide for future genetic strategies to enhance P uptake and N-fixing capacity to increase the net yield of this valuable grain legume. |
[Mh] Termos MeSH primário: |
Regulação da Expressão Gênica de Plantas/genética Micorrizas/genética Micorrizas/metabolismo Nitrogênio/metabolismo Phaseolus/genética Phaseolus/metabolismo Fósforo/metabolismo
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[Mh] Termos MeSH secundário: |
Parede Celular/química Parede Celular/metabolismo Ciclopentanos/metabolismo Fungos/fisiologia Perfilação da Expressão Gênica Oxilipinas/metabolismo Phaseolus/crescimento & desenvolvimento Phaseolus/microbiologia Raízes de Plantas/crescimento & desenvolvimento Raízes de Plantas/metabolismo Raízes de Plantas/microbiologia RNA Mensageiro/metabolismo Rhizobium/fisiologia Ácido Salicílico/metabolismo Transdução de Sinais Simbiose
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[Pt] Tipo de publicação: | JOURNAL ARTICLE |
[Nm] Nome de substância:
| 0 (Cyclopentanes); 0 (Oxylipins); 0 (RNA, Messenger); 27YLU75U4W (Phosphorus); 6RI5N05OWW (jasmonic acid); N762921K75 (Nitrogen); O414PZ4LPZ (Salicylic Acid) |
[Em] Mês de entrada: | 1710 |
[Cu] Atualização por classe: | 171016 |
[Lr] Data última revisão:
| 171016 |
[Sb] Subgrupo de revista: | IM |
[Da] Data de entrada para processamento: | 170804 |
[St] Status: | MEDLINE |
[do] DOI: | 10.1371/journal.pone.0182328 |
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