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[PMID]:28638920
[Au] Autor:Wei TJ; Chen HY; Huang X; Weng JJ; Qin JY; Su JP
[Ad] Endereço:Department of Otolaryngology Head and Neck Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China.
[Ti] Título:[A study on toxic effects of sodium salicylate on rat cochlear spiral ganglion neurons: dopamine receptors mediate expressions of NMDA and GABA receptors].
[So] Source:Sheng Li Xue Bao;69(3):285-290, 2017 Jun 25.
[Is] ISSN:0371-0874
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:The aim of the present study was to observe whether dopamine receptor (DR) was involved in the effects of sodium salicylate (SS) on the expressions of N-methyl-D-aspartic acid (NMDA) and γ-aminobutyric acid (GABA) receptors in rat cochlear spiral ganglion neurons (SGNs). Forty-eight hours after primary culture of rat SGNs, immunofluorescence technique was applied to detect expressions of DR1 and DR2, the two subtypes of dopamine receptors. Western blot was performed to assess NMDA receptor NR1 subunit and GABA receptor subunit α2 (GABRα2) protein expressions in the SGNs after the treatments of SS alone or in combination with DR antagonists. The results demonstrated that: (1) The DR1 and DR2 were expressed in the bodies and axons of the SGN; (2) After the treatment with SS, the surface protein expressions of GABRα2 and NR1 were decreased by 44.69% and 21.57%, respectively, while the total protein expressions showed no significant changes; (3) Neither SS + SCH23390 (DR1 antagonist) group nor SS + Eticlopride (DR2 antagonist) group showed significant differences in GABRα2 and NR1 surface protein expressions compared with the control group. These results suggest that SS regulates the surface GABA and NMDA receptors trafficking on SGN, and the mechanism may involve DR mediation.
[Mh] Termos MeSH primário: Receptores Dopaminérgicos/metabolismo
Receptores de GABA-A/metabolismo
Receptores de N-Metil-D-Aspartato/metabolismo
Salicilato de Sódio/toxicidade
Gânglio Espiral da Cóclea/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Benzazepinas/farmacologia
Células Cultivadas
Cóclea/citologia
Neurônios/efeitos dos fármacos
Ratos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Benzazepines); 0 (Receptors, Dopamine); 0 (Receptors, GABA-A); 0 (Receptors, N-Methyl-D-Aspartate); 0 (SCH 23390); WIQ1H85SYP (Sodium Salicylate)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171003
[Lr] Data última revisão:
171003
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170623
[St] Status:MEDLINE


  2 / 1461 MEDLINE  
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[PMID]:28088410
[Au] Autor:Carpenter AJ; Rodriguez CF; Jantz JA; Bradford BJ
[Ad] Endereço:Department of Animal Sciences and Industry, Kansas State University, Manhattan 66506. Electronic address: acarpenter@uoguelph.ca.
[Ti] Título:Short communication: Sodium salicylate negatively affects rumen fermentation in vitro and in situ.
[So] Source:J Dairy Sci;100(3):1935-1939, 2017 Mar.
[Is] ISSN:1525-3198
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Administration of sodium salicylate (SS) to cows in early lactation has a positive effect on whole-lactation milk production but a negative effect on metabolism in some cases. The objective of this trial was to determine whether SS directly affects rumen fermentation. Experiment 1 was designed to investigate the effects of direct inclusion of SS in a 24-h batch culture, and experiment 2 was designed to test the fermentative ability of rumen fluid from heifers who had received SS. In experiment 1, we combined strained and pooled rumen fluid from 3 heifers in a 2:1 ratio with McDougall's buffer, and added 150 mL of the inoculum to each flask (n = 5/treatment) with 2.5 g of fermentation substrate similar to a lactating cow ration, ground to 1 mm. We then added premixed treatments (1-mL volume) to achieve the desired final amount of SS (CON1 = 0 mg, LOW = 125 mg, MED = 250 mg, HI = 375 mg). In experiment 2, 6 heifers (n = 3/treatment) were drenched daily for 3 d, either with 62.5 g of SS dissolved in water (SAL) or an equal volume of water (CON2). Rumen fluid was collected from each heifer and was not pooled. After the fluid was mixed 2:1 with McDougall's buffer, 150 mL of inoculum was added to the fermentation flasks (n = 4/heifer) with 2.5 g of fermentation substrate. This experiment was performed the day before SS treatment began and repeated 1, 13, and 35 d after the end of the treatment period. We also performed an in situ experiment at each of these time points. In the first experiment, inclusion of SS resulted in a decrease in dry matter disappearance (DMD) over 24 h, as well as an increase in final pH. We detected no difference between treatments for gas production asymptotic volume, rate, or lag. In the second experiment, we detected a significant treatment × day interaction for DMD: we observed no difference between groups during a 24-h batch culture on the day following treatment, but SAL resulted in lower DMD on d 13 and d 35. We detected no treatment effect on the final pH of the batch culture or on any gas-production parameters. We observed a tendency for SAL to decrease the DMD rate in situ on the day after treatment. These results indicate that SS administration has a negative effect on rumen microorganisms.
[Mh] Termos MeSH primário: Lactação/efeitos dos fármacos
Rúmen/metabolismo
[Mh] Termos MeSH secundário: Ração Animal
Animais
Bovinos
Dieta/veterinária
Feminino
Fermentação
Leite/química
Salicilato de Sódio/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
WIQ1H85SYP (Sodium Salicylate)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170522
[Lr] Data última revisão:
170522
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170116
[St] Status:MEDLINE


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[PMID]:27852976
[Au] Autor:Oh J; Sinha I; Tan KY; Rosner B; Dreyfuss JM; Gjata O; Tran P; Shoelson SE; Wagers AJ
[Ad] Endereço:Department of Stem Cell and Regenerative Biology and Harvard Stem Cell Institute, Cambridge, MA 02138, USA.
[Ti] Título:Age-associated NF-κB signaling in myofibers alters the satellite cell niche and re-strains muscle stem cell function.
[So] Source:Aging (Albany NY);8(11):2871-2896, 2016 Nov 14.
[Is] ISSN:1945-4589
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Skeletal muscle is a highly regenerative tissue, but muscle repair potential is increasingly compromised with advancing age. In this study, we demonstrate that increased NF-κB activity in aged muscle fibers contributes to diminished myogenic potential of their associated satellite cells. We further examine the impact of genetic modulation of NF-κB signaling in muscle satellite cells or myofibers on recovery after damage. These studies reveal that NF-κB activity in differentiated myofibers is sufficient to drive dysfunction of muscle regenerative cells via cell-non-autonomous mechanisms. Inhibition of NF-κB, or its downstream target Phospholipase A2, in myofibers rescued muscle regenerative potential in aged muscle. Moreover, systemic administration of sodium salicylate, an FDA-approved NF-κB inhibitor, decreased inflammatory gene expression and improved repair in aged muscle. Together, these studies identify a unique NF-κB regulated, non-cell autonomous mechanism by which stem cell function is linked to lipid signaling and homeostasis, and provide important new targets to stimulate muscle repair in aged individuals.
[Mh] Termos MeSH primário: Fibras Musculares Esqueléticas/metabolismo
NF-kappa B/metabolismo
Células Satélites de Músculo Esquelético/metabolismo
[Mh] Termos MeSH secundário: Idoso
Envelhecimento/fisiologia
Animais
Anti-Inflamatórios não Esteroides/farmacologia
Senescência Celular
Expressão Gênica
Seres Humanos
Camundongos
Desenvolvimento Muscular/efeitos dos fármacos
Fibras Musculares Esqueléticas/efeitos dos fármacos
Regeneração
Células Satélites de Músculo Esquelético/efeitos dos fármacos
Salicilato de Sódio/farmacologia
Nicho de Células-Tronco
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents, Non-Steroidal); 0 (NF-kappa B); WIQ1H85SYP (Sodium Salicylate)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161118
[St] Status:MEDLINE
[do] DOI:10.18632/aging.101098


  4 / 1461 MEDLINE  
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[PMID]:27835697
[Au] Autor:Pace E; Luo H; Bobian M; Panekkad A; Zhang X; Zhang H; Zhang J
[Ad] Endereço:Department of Otolaryngology-Head and Neck Surgery, Wayne State University School of Medicine, 4201 Saint Antoine, Detroit, Michigan 48201, United States of America.
[Ti] Título:A Conditioned Behavioral Paradigm for Assessing Onset and Lasting Tinnitus in Rats.
[So] Source:PLoS One;11(11):e0166346, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Numerous behavioral paradigms have been developed to assess tinnitus-like behavior in animals. Nevertheless, they are often limited by prolonged training requirements, as well as an inability to simultaneously assess onset and lasting tinnitus behavior, tinnitus pitch or duration, or tinnitus presence without grouping data from multiple animals or testing sessions. To enhance behavioral testing of tinnitus, we developed a conditioned licking suppression paradigm to determine the pitch(s) of both onset and lasting tinnitus-like behavior within individual animals. Rats learned to lick water during broadband or narrowband noises, and to suppress licking to avoid footshocks during silence. After noise exposure, rats significantly increased licking during silent trials, suggesting onset tinnitus-like behavior. Lasting tinnitus-behavior, however, was exhibited in about half of noise-exposed rats through 7 weeks post-exposure tested. Licking activity during narrowband sound trials remained unchanged following noise exposure, while ABR hearing thresholds fully recovered and were comparable between tinnitus(+) and tinnitus(-) rats. To assess another tinnitus inducer, rats were injected with sodium salicylate. They demonstrated high pitch tinnitus-like behavior, but later recovered by 5 days post-injection. Further control studies showed that 1): sham noise-exposed rats tested with footshock did not exhibit tinnitus-like behavior, and 2): noise-exposed or sham rats tested without footshocks showed no fundamental changes in behavior compared to those tested with shocks. Together, these results demonstrate that this paradigm can efficiently test the development of noise- and salicylate-induced tinnitus behavior. The ability to assess tinnitus individually, over time, and without averaging data enables us to realistically address tinnitus in a clinically relevant way. Thus, we believe that this optimized behavioral paradigm will facilitate investigations into the mechanisms of tinnitus and development of effective treatments.
[Mh] Termos MeSH primário: Condicionamento Operante
Asseio Animal/fisiologia
Ruído/efeitos adversos
Salicilato de Sódio/efeitos adversos
Zumbido/diagnóstico
[Mh] Termos MeSH secundário: Estimulação Acústica
Animais
Modelos Animais de Doenças
Eletrochoque
Masculino
Ratos
Ratos Sprague-Dawley
Som
Zumbido/etiologia
Zumbido/fisiopatologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
WIQ1H85SYP (Sodium Salicylate)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170623
[Lr] Data última revisão:
170623
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161112
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0166346


  5 / 1461 MEDLINE  
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[PMID]:27524054
[Au] Autor:Mondal S; Dasgupta S; Maji K
[Ad] Endereço:National Institute of Technology Rourkela, Ceramic Engineering, Rourkela, India.
[Ti] Título:MgAl- Layered Double Hydroxide Nanoparticles for controlled release of Salicylate.
[So] Source:Mater Sci Eng C Mater Biol Appl;68:557-64, 2016 Nov 01.
[Is] ISSN:1873-0191
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Layered double hydroxides (LDHs), have been known for many decades as catalyst and ceramic precursors, traps for anionic pollutants, and additives for polymers. Recently, their successful synthesis on the nanometer scale opened up a whole new field for their application in nanomedicine. Here we report the efficacy of Mg1-xAlx (NO3)x (OH)2 LDH nanoparticles as a carrier and for controlled release of one of the non-steroidal anti-inflammatory drugs (NSAID), sodium salicylate. Mg1-xAlx (NO3)x (OH)2.nH2O nanoparticles were synthesized using co-precipitation method from an aqueous solution of Mg(NO3)2.6H2O and Al(NO3)3.9H2O. Salicylate was intercalated in the interlayer space of Mg-Al LDH after suspending nanoparticles in 0.0025(M) HNO3 and 0.75 (M) NaNO3 solution and using anion exchange method under N2 atmosphere. The shift in the basal planes like (003) and (006) to lower 2θ value in the XRD plot of intercalated sample confirmed the increase in basal spacing in LDH because of intercalation of salicylate into the interlayer space of LDH. FTIR spectroscopy of SA-LDH nano hybrid revealed a red shift in the frequency band of carboxylate group in salicylate indicating an electrostatic interaction between cationic LDH sheet and anionic drug. Differential thermal analysis of LDH-SA nanohybrid indicated higher thermal stability of salicylate in the intercalated form into LDH as compared to its free state. DLS studies showed a particle size distribution between 30-60 nm for pristine LDH whereas salicylate intercalated LDH exhibited a particle size distribution between 40-80nm which is ideal for its efficacy as a superior carrier for drugs and biomolecules. The cumulative release kinetic of salicylate from MgAl-LDH-SA hybrids in phosphate buffer saline (PBS) at pH7.4 showed a sustained release of salicylate up to 72h that closely resembled first order release kinetics through a combination of drug diffusion and dissolution of LDH under physiological conditions. Also the cytotoxicity tests performed revealed the less toxic nature of the nanohybrid as compared to the bare SA drug.
[Mh] Termos MeSH primário: Hidróxido de Alumínio
Hidróxido de Magnésio
Salicilato de Sódio
[Mh] Termos MeSH secundário: Hidróxido de Alumínio/química
Hidróxido de Alumínio/farmacocinética
Preparações de Ação Retardada/química
Preparações de Ação Retardada/farmacocinética
Hidróxido de Magnésio/química
Hidróxido de Magnésio/farmacocinética
Salicilato de Sódio/química
Salicilato de Sódio/farmacocinética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Delayed-Action Preparations); 5QB0T2IUN0 (Aluminum Hydroxide); NBZ3QY004S (Magnesium Hydroxide); WIQ1H85SYP (Sodium Salicylate)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170406
[Lr] Data última revisão:
170406
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160816
[St] Status:MEDLINE


  6 / 1461 MEDLINE  
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[PMID]:27490102
[Au] Autor:Gondor OK; Pál M; Darkó É; Janda T; Szalai G
[Ad] Endereço:Department of Plant Physiology, Agricultural Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, Martonvásár, Hungary.
[Ti] Título:Salicylic Acid and Sodium Salicylate Alleviate Cadmium Toxicity to Different Extents in Maize (Zea mays L.).
[So] Source:PLoS One;11(8):e0160157, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The role of salicylic acid in Cd tolerance has attracted more attention recently but no information is available on the efficiency of different forms of salicylic acid. The aim was thus to investigate whether both the acid and salt forms of salicylic acid provide protection against Cd stress and to compare their mode of action. Young maize plants were grown under controlled environmental conditions. One group of 10-day-old seedlings were treated with 0.5 mM SA or NaSA for 1 day then half of the pants were treated with 0.5 mM Cd for 1 day. Another group of seedlings was treated with 0.5 mM CdSO4 for 1 day without pre-treatment with SA or NaSA, while a third group was treated simultaneously with Cd and either SA or NaSA. Both salicylic acid forms reduced the Cd accumulation in the roots. Treatment with the acidic form meliorated the Cd accumulation in the leaves, while Na-salicylate increased the phytochelatin level in the roots and the amount of salicylic acid in the leaves. Furthermore, increased antioxidant enzyme activity was mainly induced by the acid form, while glutathione-related redox changes were influenced mostly by the salt form. The acidic and salt forms of salicylic acid affected the two antioxidant systems in different ways, and the influence of these two forms on the distribution and detoxification of Cd also differed. The present results also draw attention to the fact that generalisations about the stress protective mechanisms induced by salicylic acid are misleading since different forms of SA may exert different effects on the plants via separate mechanisms.
[Mh] Termos MeSH primário: Cádmio/toxicidade
Ácido Salicílico/farmacologia
Salicilato de Sódio/farmacologia
Zea mays/efeitos dos fármacos
[Mh] Termos MeSH secundário: Aminoaciltransferases/metabolismo
Antioxidantes/metabolismo
Ácido Ascórbico/metabolismo
Catalase/metabolismo
Clorofila/análise
Glutationa Redutase/metabolismo
Glutationa Transferase/metabolismo
Malondialdeído/análise
Estresse Oxidativo
Peroxidases/metabolismo
Fitoquelatinas/metabolismo
Folhas de Planta/química
Folhas de Planta/efeitos dos fármacos
Folhas de Planta/metabolismo
Raízes de Plantas/química
Raízes de Plantas/efeitos dos fármacos
Raízes de Plantas/metabolismo
Plântulas/efeitos dos fármacos
Espectrometria de Fluorescência
Compostos de Sulfidrila/análise
Zea mays/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Sulfhydryl Compounds); 00BH33GNGH (Cadmium); 1406-65-1 (Chlorophyll); 4Y8F71G49Q (Malondialdehyde); 98726-08-0 (Phytochelatins); EC 1.11.1.- (Peroxidases); EC 1.11.1.6 (Catalase); EC 1.8.1.7 (Glutathione Reductase); EC 2.3.2.- (Aminoacyltransferases); EC 2.3.2.15 (glutathione gamma-glutamylcysteinyltransferase); EC 2.5.1.18 (Glutathione Transferase); O414PZ4LPZ (Salicylic Acid); PQ6CK8PD0R (Ascorbic Acid); WIQ1H85SYP (Sodium Salicylate)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170823
[Lr] Data última revisão:
170823
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160805
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0160157


  7 / 1461 MEDLINE  
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[PMID]:27388640
[Au] Autor:Song Y; Liu J; Ma F; Mao L
[Ad] Endereço:a Department of Otorhinolaryngology , Peking University Third Hospital , Beijing , PR China.
[Ti] Título:Diazepam reduces excitability of amygdala and further influences auditory cortex following sodium salicylate treatment in rats.
[So] Source:Acta Otolaryngol;136(12):1220-1224, 2016 Dec.
[Is] ISSN:1651-2251
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:CONCLUSION: Diazepam can reduce the excitability of lateral amygdala and eventually suppress the excitability of the auditory cortex in rats following salicylate treatment, indicating the regulating effect of lateral amygdala to the auditory cortex in the tinnitus procedure. OBJECTIVE: To study the spontaneous firing rates (SFR) of the auditory cortex and lateral amygdala regulated by diazepam in the tinnitus rat model induced by sodium salicylate. MATERIALS AND METHODS: This study first created a tinnitus rat modal induced by sodium salicylate, and recorded SFR of both auditory cortex and lateral amygdala. Then diazepam was intraperitoneally injected and the SFR changes of lateral amygdala recorded. Finally, diazepam was microinjected on lateral amygdala and the SFR changes of the auditory cortex recorded. RESULTS: Both SFRs of the auditory cortex and lateral amygdala increased after salicylate treatment. SFR of lateral amygdala decreased after intraperitoneal injection of diazepam. Microinjecting diazepam to lateral amygdala decreased SFR of the auditory cortex ipsilaterally and contralaterally.
[Mh] Termos MeSH primário: Tonsila do Cerebelo/efeitos dos fármacos
Ansiolíticos/uso terapêutico
Córtex Auditivo/efeitos dos fármacos
Diazepam/uso terapêutico
Zumbido/tratamento farmacológico
[Mh] Termos MeSH secundário: Animais
Ansiolíticos/farmacologia
Diazepam/farmacologia
Modelos Animais de Doenças
Avaliação Pré-Clínica de Medicamentos
Masculino
Microinjeções
Ratos Wistar
Salicilato de Sódio
Zumbido/induzido quimicamente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Anxiety Agents); Q3JTX2Q7TU (Diazepam); WIQ1H85SYP (Sodium Salicylate)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170207
[Lr] Data última revisão:
170207
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160709
[St] Status:MEDLINE


  8 / 1461 MEDLINE  
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[PMID]:27001819
[Au] Autor:Zander E; Seifert H; Higgins PG
[Ad] Endereço:Institute for Medical Microbiology, Immunology, and Hygiene, University of Cologne, Cologne, Germany.
[Ti] Título:Effects of Saline, an Ambient Acidic Environment, and Sodium Salicylate on OXA-Mediated Carbapenem Resistance in Acinetobacter baumannii.
[So] Source:Antimicrob Agents Chemother;60(6):3415-8, 2016 Jun.
[Is] ISSN:1098-6596
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Different physiological conditions, such as NaCl, low pH, and sodium salicylate, have been shown to affect antibiotic resistance determinants in Acinetobacter baumannii isolates. Therefore, the aim of this study was to investigate the effects of NaCl, sodium salicylate, and low pH on the susceptibility of A. baumannii to carbapenem. We cloned genes encoding oxacillinases (OXA) of different subclasses, with their associated promoters, from carbapenem-resistant A. baumannii isolates into the same vector and transferred them to the A. baumannii reference strains ATCC 19606 and ATCC 17978. Carbapenem MICs were determined at least in triplicate by agar dilution under standard conditions, as well as in the presence of 200 mM NaCl or 16 mM sodium salicylate, or at pH 5.8. OXA-58-like gene expression was determined by reverse transcription-quantitative PCR (qRT-PCR). Under some experimental conditions, significant MIC reductions were shown for some transformants but not for others. Only in one instance were all transformants harboring the same OXA affected by the same condition: at pH 5.8, the imipenem and meropenem MICs for strains expressing OXA-58-like enzymes decreased from a resistant level (32 to 64 mg/liter) to an intermediate-susceptible level (8 mg/liter). However, blaOXA-58-like gene expression remained the same. MICs for both wild-type reference strains were not affected by the conditions tested. Our results indicate that the effects of the experimental conditions tested on OXA in vivo are mostly strain dependent. MICs were not reduced to wild-type levels, suggesting that the conditions tested do not lead to complete OXA inhibition in the bacterial cell.
[Mh] Termos MeSH primário: Acinetobacter baumannii/efeitos dos fármacos
Acinetobacter baumannii/enzimologia
Antibacterianos/farmacologia
Carbapenêmicos/farmacologia
Cloreto de Sódio/farmacologia
Salicilato de Sódio/farmacologia
beta-Lactamases/metabolismo
[Mh] Termos MeSH secundário: Acinetobacter baumannii/genética
Farmacorresistência Bacteriana/genética
Concentração de Íons de Hidrogênio
Testes de Sensibilidade Microbiana
Reação em Cadeia da Polimerase Via Transcriptase Reversa
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Carbapenems); 451W47IQ8X (Sodium Chloride); EC 3.5.2.6 (beta-Lactamases); EC 3.5.2.6 (oxacillinase); WIQ1H85SYP (Sodium Salicylate)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170915
[Lr] Data última revisão:
170915
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160323
[St] Status:MEDLINE
[do] DOI:10.1128/AAC.03010-15


  9 / 1461 MEDLINE  
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[PMID]:26980755
[Au] Autor:Song X; Walczak P; He X; Yang X; Pearl M; Bulte JW; Pomper MG; McMahon MT; Janowski M
[Ad] Endereço:Russell H. Morgan Department of Radiology and Radiological Science, The Johns Hopkins University School of Medicine, Baltmore, MD, USA F.M. Kirby Research Center for Functional Brain Imaging, Kennedy Krieger Institute, Baltimore, MD, USA.
[Ti] Título:Salicylic acid analogues as chemical exchange saturation transfer MRI contrast agents for the assessment of brain perfusion territory and blood-brain barrier opening after intra-arterial infusion.
[So] Source:J Cereb Blood Flow Metab;36(7):1186-94, 2016 Jul.
[Is] ISSN:1559-7016
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The blood-brain barrier (BBB) is a major obstacle for drug delivery to the brain. Predicted, focal opening of the BBB through intra-arterial infusion of hyperosmolar mannitol is feasible, but there is a need to facilitate imaging techniques (e.g. MRI) to guide interventional procedures and assess the outcomes. Here, we show that salicylic acid analogues (SAA) can depict the brain territory supplied by the catheter and detect the BBB opening, through chemical exchange saturation transfer (CEST) MRI. Hyperosmolar SAA solutions themselves are also capable of opening the BBB, and, when multiple SAA agents were co-injected, their locoregional perfusion could be differentiated.
[Mh] Termos MeSH primário: Barreira Hematoencefálica/diagnóstico por imagem
Meios de Contraste/química
Imagem por Ressonância Magnética Intervencionista/métodos
Imagem de Perfusão/métodos
Ácido Salicílico/química
[Mh] Termos MeSH secundário: Animais
Barreira Hematoencefálica/metabolismo
Encéfalo/irrigação sanguínea
Encéfalo/diagnóstico por imagem
Meios de Contraste/administração & dosagem
Hidroxibenzoatos/administração & dosagem
Hidroxibenzoatos/química
Infusões Intra-Arteriais
Concentração Osmolar
Perfusão
Ácidos Ftálicos/administração & dosagem
Ácidos Ftálicos/química
Ratos Sprague-Dawley
Ácido Salicílico/administração & dosagem
Salicilato de Sódio/administração & dosagem
Salicilato de Sódio/química
Soluções
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (2,5-dihydroxyterephthalic acid); 0 (Contrast Media); 0 (Hydroxybenzoates); 0 (Phthalic Acids); 0 (Solutions); LU39SC9JYL (beta-resorcylic acid); O414PZ4LPZ (Salicylic Acid); WIQ1H85SYP (Sodium Salicylate)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170720
[Lr] Data última revisão:
170720
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160317
[St] Status:MEDLINE
[do] DOI:10.1177/0271678X16637882


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[PMID]:26944926
[Au] Autor:Bhaskarla C; Das M; Verma T; Kumar A; Mahadevan S; Nandi D
[Ad] Endereço:1​Department of Biochemistry, Indian Institute of Science,Bangalore,India.
[Ti] Título:Roles of Lon protease and its substrate MarA during sodium salicylate-mediated growth reduction and antibiotic resistance in Escherichia coli.
[So] Source:Microbiology;162(5):764-76, 2016 May.
[Is] ISSN:1465-2080
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The cellular proteolytic machinery orchestrates protein turnover and regulates several key biological processes. This study addresses the roles of Lon, a major ATP-dependent protease, in modulating the responses of Escherichia coli strain MG1655 to low and high amounts of sodium salicyclate (NaSal), a widely used clinically relevant analgesic. NaSal affects several bacterial responses, including growth and resistance to multiple antibiotics. The loss of lon reduces growth in response to high, but not low, amounts of NaSal. From amongst a panel of Lon substrates, MarA was identified to be the downstream target of Lon. Thus, stabilization of MarA in the absence of lon lowers growth of the strain in the presence of higher amounts of NaSal. The steady-state transcript levels of marA and its target genes, acrA, acrB and tolC, are higher in the Δlon strain compared with the WT strain. Consequently, the resistance to antibiotics, e.g. tetracycline and nalidixic acid, is enhanced in Δlon in a marA-dependent manner. Furthermore, the target genes of MarA, i.e. acrB and tolC, are responsible for NaSal-mediated antibiotic resistance. Studies using atomic force microscopy demonstrated that ciprofloxacin led to greater cell filamentation, which is lower in the Δlon strain due to higher levels of MarA. Overall, this study delineates the roles of Lon protease, its substrate MarA and downstream targets of MarA, e.g. acrB and tolC, during NaSal-mediated growth reduction and antibiotic resistance. The implications of these observations in the adaptation of E. coli under different environmental conditions are discussed.
[Mh] Termos MeSH primário: Proteínas de Ligação a DNA/metabolismo
Farmacorresistência Bacteriana Múltipla/genética
Proteínas de Escherichia coli/metabolismo
Escherichia coli/crescimento & desenvolvimento
Protease La/metabolismo
Salicilato de Sódio/farmacologia
[Mh] Termos MeSH secundário: Antibacterianos/farmacologia
Proteínas da Membrana Bacteriana Externa/genética
Ciprofloxacino/farmacologia
Escherichia coli/genética
Proteínas de Escherichia coli/genética
Lipoproteínas/genética
Proteínas de Membrana Transportadoras/genética
Testes de Sensibilidade Microbiana
Microscopia de Força Atômica
Proteínas Associadas à Resistência a Múltiplos Medicamentos/genética
Ácido Nalidíxico/farmacologia
Protease La/genética
Salicilato de Sódio/metabolismo
Tetraciclina/farmacologia
Resistência a Tetraciclina/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (AcrA protein, E coli); 0 (AcrB protein, E coli); 0 (Anti-Bacterial Agents); 0 (Bacterial Outer Membrane Proteins); 0 (DNA-Binding Proteins); 0 (Escherichia coli Proteins); 0 (Lipoproteins); 0 (MarA protein, E coli); 0 (Membrane Transport Proteins); 0 (Multidrug Resistance-Associated Proteins); 0 (tolC protein, E coli); 3B91HWA56M (Nalidixic Acid); 5E8K9I0O4U (Ciprofloxacin); EC 3.4.21.53 (Lon protein, E coli); EC 3.4.21.53 (Protease La); F8VB5M810T (Tetracycline); WIQ1H85SYP (Sodium Salicylate)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170915
[Lr] Data última revisão:
170915
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160306
[St] Status:MEDLINE
[do] DOI:10.1099/mic.0.000271



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