Base de dados : MEDLINE
Pesquisa : D02.241.511.459 [Categoria DeCS]
Referências encontradas : 32776 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 3278 ir para página                         

  1 / 32776 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29311546
[Au] Autor:Yang JE; Park SJ; Kim WJ; Kim HJ; Kim BJ; Lee H; Shin J; Lee SY
[Ad] Endereço:Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 Plus Program), Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, 34141, Repu
[Ti] Título:One-step fermentative production of aromatic polyesters from glucose by metabolically engineered Escherichia coli strains.
[So] Source:Nat Commun;9(1):79, 2018 01 08.
[Is] ISSN:2041-1723
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Aromatic polyesters are widely used plastics currently produced from petroleum. Here we engineer Escherichia coli strains for the production of aromatic polyesters from glucose by one-step fermentation. When the Clostridium difficile isocaprenoyl-CoA:2-hydroxyisocaproate CoA-transferase (HadA) and evolved polyhydroxyalkanoate (PHA) synthase genes are overexpressed in a D-phenyllactate-producing strain, poly(52.3 mol% 3-hydroxybutyrate (3HB)-co-47.7 mol% D-phenyllactate) can be produced from glucose and sodium 3HB. Also, various poly(3HB-co-D-phenyllactate) polymers having 11.0, 15.8, 20.0, 70.8, and 84.5 mol% of D-phenyllactate are produced from glucose as a sole carbon source by additional expression of Ralstonia eutropha ß-ketothiolase (phaA) and reductase (phaB) genes. Fed-batch culture of this engineered strain produces 13.9 g l of poly(61.9 mol% 3HB-co-38.1 mol% D-phenyllactate). Furthermore, different aromatic polyesters containing D-mandelate and D-3-hydroxy-3-phenylpropionate are produced from glucose when feeding the corresponding monomers. The engineered bacterial system will be useful for one-step fermentative production of aromatic polyesters from renewable resources.
[Mh] Termos MeSH primário: Escherichia coli/metabolismo
Fermentação
Glucose/metabolismo
Engenharia Metabólica/métodos
Poliésteres/metabolismo
[Mh] Termos MeSH secundário: Ácido 3-Hidroxibutírico/metabolismo
Acetil-CoA C-Aciltransferase/genética
Acetil-CoA C-Aciltransferase/metabolismo
Aciltransferases/genética
Aciltransferases/metabolismo
Proteínas de Bactérias/genética
Proteínas de Bactérias/metabolismo
Clostridium difficile/enzimologia
Clostridium difficile/genética
Coenzima A-Transferases/genética
Coenzima A-Transferases/metabolismo
Cupriavidus necator/enzimologia
Cupriavidus necator/genética
Escherichia coli/genética
Lactatos/metabolismo
Oxirredutases/genética
Oxirredutases/metabolismo
Polietilenotereftalatos/metabolismo
Polímeros/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Lactates); 0 (Polyesters); 0 (Polyethylene Terephthalates); 0 (Polymers); 156-05-8 (3-phenyllactic acid); EC 1.- (Oxidoreductases); EC 2.3.- (Acyltransferases); EC 2.3.1.- (poly(3-hydroxyalkanoic acid) synthase); EC 2.3.1.16 (Acetyl-CoA C-Acyltransferase); EC 2.8.3.- (Coenzyme A-Transferases); IY9XDZ35W2 (Glucose); TZP1275679 (3-Hydroxybutyric Acid)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180110
[St] Status:MEDLINE
[do] DOI:10.1038/s41467-017-02498-w


  2 / 32776 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29253861
[Au] Autor:Eich G; Bartosova M; Tischer C; Wlodkowski TT; Schaefer B; Pichl S; Kraewer N; Ranchin B; Vondrak K; Liebau MC; Hackert T; Schmitt CP
[Ad] Endereço:Center for Pediatric and Adolescent Medicine, University Hospital Heidelberg, Heidelberg, Germany.
[Ti] Título:Bicarbonate buffered peritoneal dialysis fluid upregulates angiopoietin-1 and promotes vessel maturation.
[So] Source:PLoS One;12(12):e0189903, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Ultrafiltration decline is a progressive issue for patients on chronic peritoneal dialysis (PD) and can be caused by peritoneal angiogenesis induced by PD fluids. A recent pediatric trial suggests better preservation of ultrafiltration with bicarbonate versus lactate buffered fluid; underlying molecular mechanisms are unknown. METHODS: Angiogenic cytokine profile, tube formation capacity and Receptor Tyrosine Kinase translocation were assessed in primary human umbilical vein endothelial cells following incubation with bicarbonate (BPDF) and lactate buffered (LPDF), pH neutral PD fluid with low glucose degradation product content and lactate buffered, acidic PD fluid with high glucose degradation product content (CPDF). Peritoneal biopsies from age-, PD-vintage- and dialytic glucose exposure matched, peritonitis-free children on chronic PD underwent automated histomorphometry and immunohistochemistry. RESULTS: In endothelial cells angiopoietin-1 mRNA and protein abundance increased 200% upon incubation with BPDF, but decreased by 70% with LPDF as compared to medium control; angiopoietin-2 remained unchanged. Angiopoietin-1/Angiopoietin-2 protein ratio was 15 and 3-fold increased with BPDF compared to LPDF and medium. Time-lapse microscopy with automated network analysis demonstrated less endothelial cell tube formation with BPDF compared to LPDF and CPDF incubation. Receptor Tyrosine Kinase translocated to the cell membrane in BPDF but not in LPDF or CPDF incubated endothelial cells. In children dialyzed with BPDF peritoneal vessels were larger and angiopoietin-1 abundance in CD31 positive endothelium higher compared to children treated with LPDF. CONCLUSION: Bicarbonate buffered PD fluid promotes vessel maturation via upregulation of angiopoietin-1 in vitro and in children on dialysis. Our findings suggest a molecular mechanism for the observed superior preservation of ultrafiltration capacity with bicarbonate buffered PD fluid with low glucose degradation product content.
[Mh] Termos MeSH primário: Angiopoietina-1/metabolismo
Bicarbonatos/química
Tampões (Química)
Diálise Peritoneal
[Mh] Termos MeSH secundário: Adolescente
Angiopoietina-2/metabolismo
Biópsia
Criança
Doença Crônica
Citocinas/metabolismo
Células Endoteliais/metabolismo
Glucose/química
Células Endoteliais da Veia Umbilical Humana
Seres Humanos
Concentração de Íons de Hidrogênio
Nefropatias/terapia
Lactatos/química
Peritônio/patologia
Molécula-1 de Adesão Celular Endotelial de Plaquetas/metabolismo
Receptores Proteína Tirosina Quinases/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (ANGPT1 protein, human); 0 (ANGPT2 protein, human); 0 (Angiopoietin-1); 0 (Angiopoietin-2); 0 (Bicarbonates); 0 (Buffers); 0 (Cytokines); 0 (Lactates); 0 (Platelet Endothelial Cell Adhesion Molecule-1); EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases); IY9XDZ35W2 (Glucose)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180112
[Lr] Data última revisão:
180112
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171219
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189903


  3 / 32776 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
[PMID]:29182252
[Au] Autor:Arroyo D
[Ti] Título:EtCO2 and Lactate: Correlation may be key to early sepsis detection.
[So] Source:JEMS;41(9):48-9, 2016 09.
[Is] ISSN:0197-2510
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Capnografia
Serviços Médicos de Emergência/organização & administração
Lactatos/sangue
Sepse/diagnóstico
Sepse/terapia
[Mh] Termos MeSH secundário: Biomarcadores/sangue
Seres Humanos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 0 (Lactates)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180108
[Lr] Data última revisão:
180108
[Sb] Subgrupo de revista:H
[Da] Data de entrada para processamento:171129
[St] Status:MEDLINE


  4 / 32776 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28973039
[Au] Autor:Casuso RA; Plaza-Díaz J; Ruiz-Ojeda FJ; Aragón-Vela J; Robles-Sanchez C; Nordsborg NB; Hebberecht M; Salmeron LM; Huertas JR
[Ad] Endereço:Department of Physiology, School of Pharmacy, University of Granada, Granada, Spain.
[Ti] Título:High-intensity high-volume swimming induces more robust signaling through PGC-1α and AMPK activation than sprint interval swimming in m. triceps brachii.
[So] Source:PLoS One;12(10):e0185494, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We aimed to test whether high-intensity high-volume training (HIHVT) swimming would induce more robust signaling than sprint interval training (SIT) swimming within the m. triceps brachii due to lower metabolic and oxidation. Nine well-trained swimmers performed the two training procedures on separate randomized days. Muscle biopsies from m. triceps brachii and blood samples were collected at three different time points: a) before the intervention (pre), b) immediately after the swimming procedures (post) and c) after 3 h of rest (3 h). Hydroperoxides, creatine kinase (CK), and lactate dehydrogenase (LDH) were quantified from blood samples, and peroxisome proliferator-activated receptor γ coactivator 1α (PGC-1α) and the AMPKpTHR172/AMPK ratio were quantified by Western blot analysis. PGC-1α, sirtuin 3 (SIRT3), superoxide-dismutase 2 (SOD2), and vascular endothelial growth factor (VEGF) mRNA levels were also quantified. SIT induced a higher release of LDH (p < 0.01 at all time points) and CK (p < 0.01 at post) than HIHVT, but neither SIT nor HIHVT altered systemic hydroperoxides. Additionally, neither SIRT3 nor SOD2 mRNA levels increased, while PGC-1α transcription increased at 3 h after SIT (p < 0.01) and after HIHVT (p < 0.001). However, PGC-1α protein was higher after HIHVT than after SIT (p < 0.05). Moreover, the AMPKpTHR172/AMPK ratio increased at post after SIT (p < 0.05), whereas this effect was delayed after HIHVT as it increased after 3 h (p < 0.05). In addition, VEGF transcription was higher in response to HIHVT (p < 0.05). In conclusion, SIT induces higher muscular stress than HIHVT without increasing systemic oxidation. In addition, HIHVT may induce more robust oxidative adaptations through PGC-1α and AMPK.
[Mh] Termos MeSH primário: Proteínas Quinases Ativadas por AMP/metabolismo
Músculo Esquelético/fisiologia
Coativador 1-alfa do Receptor gama Ativado por Proliferador de Peroxissomo/metabolismo
Transdução de Sinais
Natação
[Mh] Termos MeSH secundário: Antioxidantes/metabolismo
Western Blotting
Frequência Cardíaca
Seres Humanos
Lactatos/sangue
Peroxidação de Lipídeos
Masculino
Músculo Esquelético/metabolismo
Estresse Oxidativo
RNA Mensageiro/genética
Reação em Cadeia da Polimerase em Tempo Real
Transcrição Genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Lactates); 0 (PPARGC1A protein, human); 0 (Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha); 0 (RNA, Messenger); EC 2.7.11.31 (AMP-Activated Protein Kinases)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171004
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0185494


  5 / 32776 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28972976
[Au] Autor:Moran JL; Santamaria J
[Ad] Endereço:Department of Intensive Care Medicine, The Queen Elizabeth Hospital, Woodville, South Australia, Australia.
[Ti] Título:Reconsidering lactate as a sepsis risk biomarker.
[So] Source:PLoS One;12(10):e0185320, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVES: There has been renewed interest in lactate as a risk biomarker in sepsis and septic shock. However, the ability of the odds ratio (OR) and change in the area under the receiver operator characteristic curve (AUC-ROC) to assess biomarker added-value has been questioned. DESIGN, SETTING AND PARTICIPANTS: A sepsis cohort was identified from the ICU database of an Australian tertiary referral hospital using APACHE III diagnostic codes. Demographic information, APACHE III scores, 24-hour post-admission patient lactate levels, and hospital mortality were accessed. MEASUREMENTS AND MAIN RESULTS: Hospital mortality was modelled using a base predictive logistic regression model and sequential addition of admission lactate, lactate clearance ([lactateadmission-lactatefinal]/lactateadmission), and area under the lactate-time curve (LTC). Added-value was assessed using lactate index OR; AUC-ROC difference (base-model versus lactate index addition); net (mortality) reclassification index (NRI; range -2 to +2); and net benefit (NB), the number of true positives per patient adjusted for the number of false positives. The data set comprised 717 patients with mean(SD) age and APACHE III score 61.1(16.5) years and 68.3(28.2) respectively; 59.2% were male. Admission lactate was 2.3(2.5) mmol/l; with lactate of ≥ 4 mmol/L (37% hospital mortality) in 17% and patients with lactate < 4 mmol/L having 18% hospital mortality. The admission base-model had an AUC-ROC = 0.81 with admission lactate OR = 1.127 (95%CI: 1.038, 1.224), AUC-ROC difference of 0.0032 (-0.0037, 0.01615; P = 0.61), and NRI 0.240(0.030, 0.464). The over-time model had an AUC-ROC = 0.86 with (i) clearance OR = 0.771, 95%CI: 0.578, 1.030; P = 0.08; AUC-ROC difference 0.001 (-0.003, 0.014; P = 0.78), and NRI 0.109(-0.193, 0.425) and (ii) LTC OR = 0.997, 95%CI: 0.989, 1.005, P = 0.49; AUC-ROC difference 0.004 (-0.002, 0.004; P = 0.34), and NRI 0.111(-0.222, 0.403). NB was not incremented by any lactate index. CONCLUSIONS: Lactate added-value assessment is dependent upon the performance of the underlying predictive model and should incorporate risk reclassification and net benefit measures.
[Mh] Termos MeSH primário: Biomarcadores/metabolismo
Lactatos/metabolismo
Sepse/epidemiologia
[Mh] Termos MeSH secundário: APACHE
Adulto
Idoso
Feminino
Seres Humanos
Masculino
Meia-Idade
Fatores de Risco
Sepse/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 0 (Lactates)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171004
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0185320


  6 / 32776 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28893092
[Au] Autor:Yuan T; Chen Y; Zhang H; Fang L; Du G
[Ad] Endereço:* Beijing Key Laboratory of Drug Targets Identification and Drug Screening, Union Medical College, Beijing 100050, China.
[Ti] Título:Salvianolic Acid A, a Component of Salvia miltiorrhiza, Attenuates Endothelial-Mesenchymal Transition of HPAECs Induced by Hypoxia.
[So] Source:Am J Chin Med;45(6):1185-1200, 2017.
[Is] ISSN:0192-415X
[Cp] País de publicação:Singapore
[La] Idioma:eng
[Ab] Resumo:Salvianolic acid A (SAA), a polyphenols acid, is a bioactive ingredient from a traditional Chinese medicine called Dan shen (Salvia Miltiorrhiza Bunge). According to previous studies, it was shown to have various effects such as anti-oxidative stress, antidiabetic complications and antipulmonary hypertension. This study aimed to investigate the effect of SAA on pulmonary arterial endothelial-mesenchymal transition (EndoMT) induced by hypoxia and the underlying mechanisms. Primary cultured human pulmonary arterial endothelial cells (HPAECs) were exposed to 1% O for 48[Formula: see text]h with or without SAA treatment. SAA treatment improved the morphology of HPAECs and inhibited the cytoskeleton remodeling. A total of 3[Formula: see text][Formula: see text]M SAA reduced migration distances from 262.2[Formula: see text][Formula: see text]m to 198.4[Formula: see text][Formula: see text]m at 24[Formula: see text]h and 344.8[Formula: see text][Formula: see text]m to 109.3[Formula: see text][Formula: see text]m at 48[Formula: see text]h. It was observed that the production of ROS in cells was significantly reduced by the treatment of 3[Formula: see text][Formula: see text]M SAA. Meanwhile, SAA alleviated the loss of CD31 and slightly inhibited the expression of [Formula: see text]-SMA. The mechanisms study shows that SAA treatment increased the phosphorylation levels of Smad1/5, but inhibited that of Smad2/3. Furthermore, SAA attenuated the phosphorylation levels of ERK and Cofilin, which were enhanced by hypoxia. Based on these results, our study indicated that SAA treatment can protect HPAECs from endoMT induced by hypoxia, which may perform via the inhibition on ROS production and further through the downstream effectors of BMPRs or TGF[Formula: see text]R including Smads, ERK and ROCK/cofilin pathways.
[Mh] Termos MeSH primário: Ácidos Cafeicos/farmacologia
Células Endoteliais/patologia
Transição Epitelial-Mesenquimal/efeitos dos fármacos
Hipóxia/patologia
Lactatos/farmacologia
Salvia miltiorrhiza/química
[Mh] Termos MeSH secundário: Fatores de Despolimerização de Actina/metabolismo
Movimento Celular/efeitos dos fármacos
Células Cultivadas
Citoesqueleto/patologia
Células Endoteliais/citologia
Células Endoteliais/metabolismo
Seres Humanos
Proteínas Quinases Ativadas por Mitógeno/metabolismo
Fosforilação/efeitos dos fármacos
Artéria Pulmonar/citologia
Espécies Reativas de Oxigênio/metabolismo
Proteína Smad1/metabolismo
Proteína Smad2/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Actin Depolymerizing Factors); 0 (Caffeic Acids); 0 (Lactates); 0 (Reactive Oxygen Species); 0 (SMAD1 protein, human); 0 (SMAD2 protein, human); 0 (Smad1 Protein); 0 (Smad2 Protein); 51622542XO (salvianolic acid A); EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170913
[St] Status:MEDLINE
[do] DOI:10.1142/S0192415X17500653


  7 / 32776 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28859156
[Au] Autor:Barman S; Ghosh R; Sengupta S; Mandal NC
[Ad] Endereço:Mycology and Plant Pathology Laboratory, Department of Botany, Visva-Bharati, Santiniketan, West Bengal, India.
[Ti] Título:Longterm storage of post-packaged bread by controlling spoilage pathogens using Lactobacillus fermentum C14 isolated from homemade curd.
[So] Source:PLoS One;12(8):e0184020, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:One potent lactic acid bacterial strain C14 with strong antifungal activity was isolated from homemade curd. Based on morphological as well as biochemical characters and 16S rDNA sequence homology the strain was identified as Lactobacillus fermentum. It displayed a wide antimicrobial spectrum against both Gram-positive and Gram-negative pathogenic bacteria, and also against number of food spoilage, plant and human pathogenic fungi. The cell free supernatant (CFS) of the strain C14 was also effective against the fungi tested. Inhibition of radial growth of Penicillium digitatum, Trichophyton rubrum and Mucor sp. was noticed in the presence of CFS of C14 even at low concentration (1%). More than 94.3 ± 1.6% and 91.5 ± 2.2% inhibition of conidial germination of P. digitatum and Mucor sp. were noticed in the presence of 10-fold-concentrated CFS of C14. Massive deformation of the fungal mycelia was observed by SEM studies, and losses of cellular proteins and DNA are also evident upon its treatment with C14. HPLC analysis revealed the presence of phenyl lactic acid, lactic acid along with some unidentified compounds in the antifungal extract. Challenge experiment showed immense potential of the strain C14 in preventing the spoilage of bread samples caused by Mucor sp. and Bacillus subtilis. The bread samples remained fresh upto 25 days even after inoculation with Mucor sp. (3.7 × 104 spores /ml) and B. subtilis (4.6 × 104 CFU /ml). Along with the antifungal properties, the isolated lactic acid bacterial strain also showed very good antioxidant activities. Unchanged level of liver enzymes serum glutamic pyruvic transaminase and serum glutamic oxaloacetic transaminase in albino mice upon feeding with C14 also suggested non-toxic nature of the bacterial isolate.
[Mh] Termos MeSH primário: Anti-Infecciosos/farmacologia
Antibiose
Conservantes de Alimentos/farmacologia
Lactatos/farmacologia
Ácido Láctico/farmacologia
Lactobacillus fermentum/química
Leite/microbiologia
[Mh] Termos MeSH secundário: Alanina Transaminase/metabolismo
Animais
Anti-Infecciosos/isolamento & purificação
Aspartato Aminotransferases/sangue
Pão
Fermentação
Conservantes de Alimentos/isolamento & purificação
Armazenamento de Alimentos/métodos
Bactérias Gram-Negativas/efeitos dos fármacos
Bactérias Gram-Negativas/crescimento & desenvolvimento
Bactérias Gram-Positivas/efeitos dos fármacos
Bactérias Gram-Positivas/crescimento & desenvolvimento
Seres Humanos
Lactatos/isolamento & purificação
Ácido Láctico/isolamento & purificação
Lactobacillus fermentum/isolamento & purificação
Masculino
Camundongos
Mucor/efeitos dos fármacos
Mucor/crescimento & desenvolvimento
Micélio/efeitos dos fármacos
Micélio/crescimento & desenvolvimento
Penicillium/efeitos dos fármacos
Penicillium/crescimento & desenvolvimento
Trichophyton/efeitos dos fármacos
Trichophyton/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Infective Agents); 0 (Food Preservatives); 0 (Lactates); 156-05-8 (3-phenyllactic acid); 33X04XA5AT (Lactic Acid); EC 2.6.1.1 (Aspartate Aminotransferases); EC 2.6.1.2 (Alanine Transaminase)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170901
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0184020


  8 / 32776 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28841674
[Au] Autor:Kilbride P; Lamb S; Gibbons S; Bundy J; Erro E; Selden C; Fuller B; Morris J
[Ad] Endereço:Asymptote, General Electric Healthcare, Cambridge, United Kingdom.
[Ti] Título:Cryopreservation and re-culture of a 2.3 litre biomass for use in a bioartificial liver device.
[So] Source:PLoS One;12(8):e0183385, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:For large and complex tissue engineered constructs to be available on demand, long term storage using methods, such as cryopreservation, are essential. This study optimised parameters such as excess media concentration and warming rates and used the findings to enable the successful cryopreservation of 2.3 litres of alginate encapsulated liver cell spheroids. This volume of biomass is typical of those required for successful treatment of Acute Liver Failure using our Bioartificial Liver Device. Adding a buffer of medium above the biomass, as well as slow (0.6°C/min) warming rates was found to give the best results, so long as the warming through the equilibrium melting temperature was rapid. After 72 h post thaw-culture, viable cell number, glucose consumption, lactate production, and alpha-fetoprotein production had recovered to pre-freeze values in the 2.3 litre biomass (1.00 ± 0.05, 1.19 ± 0.10, 1.23 ± 0.18, 2.03 ± 0.04 per ml biomass of the pre-cryopreservation values respectively). It was also shown that further improvements in warming rates of the biomass could reduce recovery time to < 48 h. This is the first example of a biomass of this volume being successfully cryopreserved in a single cassette and re-cultured. It demonstrates that a bioartificial liver device can be cryopreserved, and has wider applications to scale-up large volume cryopreservation.
[Mh] Termos MeSH primário: Biomassa
Criopreservação/métodos
Fígado Artificial
[Mh] Termos MeSH secundário: Reatores Biológicos
Glucose/metabolismo
Células Hep G2
Temperatura Alta
Seres Humanos
Lactatos/metabolismo
alfa-Fetoproteínas/biossíntese
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Lactates); 0 (alpha-Fetoproteins); IY9XDZ35W2 (Glucose)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171019
[Lr] Data última revisão:
171019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170826
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0183385


  9 / 32776 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28829838
[Au] Autor:Goto K; Sumi D; Kojima C; Ishibashi A
[Ad] Endereço:Graduate School of Sports and Health Science, Ritsumeikan University, Kusatsu, Shiga, Japan.
[Ti] Título:Post-exercise serum hepcidin levels were unaffected by hypoxic exposure during prolonged exercise sessions.
[So] Source:PLoS One;12(8):e0183629, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The purpose of the present study was to determine the influence of hypoxic exposure during prolonged endurance exercise sessions (79 min in total) on post-exercise hepcidin levels in trained male endurance athletes. Ten endurance athletes (mean ± standard deviation; height: 169.8 ± 7.1 cm, weight: 57.1 ± 5.0 kg) conducted two endurance exercise sessions under either a normobaric hypoxic condition [inspired O2 fraction (FiO2) = 14.5%] or a normoxic condition (FiO2 = 20.9%). Exercise consisted of 10 × 3 min running on a treadmill at 95% of maximal oxygen uptake ([Formula: see text]) with 60s of active rest at 60% of [Formula: see text]. After 10 min of rest, they subsequently performed 30 min of continuous running at 85% of [Formula: see text]. Running velocities were significantly lower in the HYPO than in the NOR (P < 0.0001). Exercise-induced blood lactate elevation was significantly greater in the HYPO (P < 0.01). There were significant increases in plasma interleukin-6, serum iron, and blood glucose levels after exercise, with no significant difference between the trials [interaction (trial × time) or main effect for trial, P > 0.05]. Serum hepcidin levels increased significantly 120 min after exercise (HYPO: from 10.7 ± 9.4 ng/mL to 15.8 ± 11.2 ng/mL; NOR: from 7.9 ± 4.7 ng/mL to 13.2 ± 7.9 ng/mL, P < 0.05), and no difference was observed between the trials. In conclusion, endurance exercise at lower running velocity in hypoxic conditions resulted in similar post-exercise hepcidin elevations as higher running velocity in normoxic conditions.
[Mh] Termos MeSH primário: Hepcidinas/sangue
Hipóxia/sangue
Corrida
[Mh] Termos MeSH secundário: Adolescente
Adulto
Glicemia/metabolismo
Seres Humanos
Interleucina-6/sangue
Ferro/sangue
Lactatos/sangue
Masculino
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Blood Glucose); 0 (Hepcidins); 0 (Interleukin-6); 0 (Lactates); E1UOL152H7 (Iron)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171013
[Lr] Data última revisão:
171013
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170823
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0183629


  10 / 32776 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28763497
[Au] Autor:Gouveia LR; Santos JC; Silva RD; Batista AD; Domingues ALC; Lopes EPA; Silva RO
[Ad] Endereço:Postgraduate Program in Chemistry, Fundamental Chemistry Department, Center for Exact and Natural Sciences, Universidade Federal de Pernambuco (UFPE), Recife, Pernambuco, Brazil.
[Ti] Título:Diagnosis of coinfection by schistosomiasis and viral hepatitis B or C using 1H NMR-based metabonomics.
[So] Source:PLoS One;12(8):e0182196, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Diagnosis of liver involvement due to schistosomiasis in asymptomatic patients from endemic areas previously diagnosed with chronic hepatitis B (HBV) or C (HCV) and periportal fibrosis is challenging. H-1 Nuclear Magnetic Resonance (NMR)-based metabonomics strategy is a powerful tool for providing a profile of endogenous metabolites of low molecular weight in biofluids in a non-invasive way. The aim of this study was to diagnose periportal fibrosis due to schistosomiasis mansoni in patients with chronic HBV or HCV infection through NMR-based metabonomics models. METHODOLOGY/PRINCIPAL FINDINGS: The study included 40 patients divided into two groups: (i) 18 coinfected patients with schistosomiasis mansoni and HBV or HCV; and (ii) 22 HBV or HCV monoinfected patients. The serum samples were analyzed through H-1 NMR spectroscopy and the models were based on Principal Component Analysis (PCA) and Partial Least Squares-Discriminant Analysis (PLS-DA). Ultrasonography examination was used to ascertain the diagnosis of periportal fibrosis. Exploratory analysis showed a clear separation between coinfected and monoinfected samples. The supervised model built from PLS-DA showed accuracy, R2 and Q2 values equal to 100%, 98.1% and 97.5%, respectively. According to the variable importance in the projection plot, lactate serum levels were higher in the coinfected group, while the signals attributed to HDL serum cholesterol were more intense in the monoinfected group. CONCLUSIONS/SIGNIFICANCE: The metabonomics models constructed in this study are promising as an alternative tool for diagnosis of periportal fibrosis by schistosomiasis in patients with chronic HBV or HCV infection from endemic areas for Schistosoma mansoni.
[Mh] Termos MeSH primário: Hepatite B Crônica/complicações
Hepatite C Crônica/complicações
Espectroscopia de Ressonância Magnética
Esquistossomose mansoni/complicações
[Mh] Termos MeSH secundário: Adulto
Idoso
Idoso de 80 Anos ou mais
Animais
Coinfecção/parasitologia
Coinfecção/virologia
Feminino
Fibrose/patologia
Seres Humanos
Lactatos/sangue
Análise dos Mínimos Quadrados
Masculino
Metabolômica
Meia-Idade
Análise de Componente Principal
Schistosoma mansoni
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Lactates)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171010
[Lr] Data última revisão:
171010
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170802
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0182196



página 1 de 3278 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde